Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0010144

Physical Location & Seq

Genomic Coordinates Forward (+)

6910284 .. 6914370

Gene Structure 4087 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1099100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1677 bp

ATGACTTACAGTCCGAAGCGTGCCATGGCGGAGCTCAGGCACTCCATCTCGGTGGGGGCTCGAGCCACTTCTTCTCCAATGAAGCGCGACGAGGACTCCTCCCCTCTGATATTCGACACTCAGCCCCAAGACGACGACGACGGCCGCGGTCGCCCTCCCTACAAAGATCGCGACCGTCCCTTCCTCTCCCATTTCCAATCCATCTGCCCCTCAATTGCCGACGACTCCAACAGCTCCAGGATCTCACTCTTCGTCGTCATCGTCGTCGCAGTCTTCTGCCTGGTCTCGATTCTGGCGATCGTGAAACGAGTGAATACTCCTTACTTGTGTAAAAAAGATGGCATTACTCTTCACTGTCCTCATGTCAAGGAATCGCCTTCGCTTTGGGAGAATCCGTATTCAGCAACCACGTCTTGGAAGTCCTGTGCCGAGCGCCGCCTTGGTGGCATTTCAGATCTTCCACCTGAAAACGAAACAACTGGGTACATATTTATTCATGCTGAGGGTGGCCTAAATCAGCAAAGAATTGCAATATGCAATGCAGTAGCTGTAGCCAAAATAATGAATGCCACCCTTATTTTGCCTGTGCTGAAGCAGGACCAGATTTGGAAAGACCAAACGAAATTTGAAGATATCTTTGATGTTGACCATTTCATTGATTACCTGAAGGATGATGTACGAATTGTTCGTGATATTCCTGAGTGGTTTACTGACAAAGCTGAGCTATTTACAAGTATAAGACGAACAGTAAAGAACATTCCAAAATATGCACCTGCTCAATTTTACATAGACAATGTTCTGCCGCGAATCAAGGAGAAGAAGATAATGGCCCTAAAACCTTTTGTCGATCGACTCGGGTACGACAATGTCCCTCAAGAAATCAACAGGCTAAGGTGCAGGGTGAATTATCATGCTCTTAAATTTCTACCTGAGATAGAGGAGATGGCTGATTTATTGGCGTCAAGGATGAGAAATCGCACTGGAGGTTCCAATCCTTATATGGCTCTTCATCTTAGGTTTGAGAAAGGGATGGTAGGCCTATCTTTCTGTGATTTTGTGGGCACAAGGGAAGAGAAAGCTCTAATGGCAGAATACAGACATAAAGAATGGCCAAGACGGTACAAGAATGGTACCCATCTATGGCAACTAGCCCTGCAGAAGCGAAAGGAAGGGCGATGCCCCCTTGAGCCTGGGGAAGTGGCTGTCATTCTTCGAGCAATGGGCTATCCTAAGGAAACTCAAATTTATGTTGCTTCAGGACAGGTTTATGGTGGCCACAACAGGATGGCGCCGTTGAGGAACATGTTCCCAAATCTGGTAACGAAAGAGGAGTTGGCGGCTAAGCAGGAGTTGGATAAATTCAGGAAGCATGTGACTAGCTTGGCAGCGCTTGACTTCCTGGTATGCTTGAAGTCTGATGTATTCGTGATGACCCACGGAGGCAACTTTGCTAAGCTGATCATAGGGGCACGCCGGTACATGGGTCATCGTCAAAAATCAATAAAACCAGACAAGGGGCTGATGTCTAAATCTTTTGGGGACCCTTACATGGGATGGGCCACCTTTGCAGACGATGTAGTGACTACCCACGAAACACGAACTGGATTACCAGAAGCGACCTTCCCTAACTATGACTTGTGGGAGAACCCTCTAACTCCTTGCATGTGTAAAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009664 GO:0009832 GO:0009987 GO:0010383 GO:0010393 GO:0012505 GO:0016043 GO:0016051 GO:0017144 GO:0031410 GO:0031982 GO:0031984 GO:0033692 GO:0034637 GO:0034645 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0045488 GO:0045489 GO:0052325 GO:0052546 GO:0070589 GO:0070592 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0097708 GO:0098791 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

O-FucT

Protein Analysis

559

Amino Acids

63.69

Weight (kDa)

8.98

Isoelectric Point (pI)

46.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
O-FucT	PF10250	162 - 494	3.2e-100	GDP-fucose protein O-fucosyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010144)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G26370 AT3G26370
fragaria_vesca	FvH4_4g25921
malus_domestica	MD13G1098200.v1.1 MD16G1099100.v1.1
prunus_persica	Prupe.1G247100_v2.0.a1
pyrus_communis	pycom13g08580 pycom13g08590 pycom16g08440 pycom16g08450
rosa_chinensis	RchiOBHm_Chr4g0433391
rosa_laevigata	RLG00000006757
rosa_multiflora	Rmu_sc0000759.1_g000007
rosa_roxburghii	Rroxscaffold_5G00374940
rosa_rugosa	Rorug04G0268400
rosa_samantha	Rh4AG323200 Rh4BG330800 Rh4BG330900 Rh4CG346200 Rh4DG327300
rosa_wichuraiana	Rw4G027990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	781
Acc36I	ACCTGC	1 cut(s)	781
Acc65I	GGTACC	1 cut(s)	1130
AccB1I	GGYRCC	2 cut(s)	1130, 1288
AccB7I	CCANNNNNTGG	1 cut(s)	414
AccII	CGCG	4 cut(s)	87, 147, 171, 805
AciI	CCGC	6 cut(s)	29, 145, 147, 436, 803, 1337
AclWI	GGATC	1 cut(s)	248
AcoI	YGGCCR	3 cut(s)	142, 1109, 1273
AcsI	RAATTY	4 cut(s)	623, 920, 1242, 1358
AcuI	CTGAAG	3 cut(s)	611, 686, 1239
AcyI	GRCGYC	2 cut(s)	959, 1289
AfaI	GTAC	6 cut(s)	485, 678, 860, 1121, 1132, 1478
AfeI	AGCGCT	1 cut(s)	1389
AfiI	CCNNNNNNNGG	7 cut(s)	414, 1140, 1315, 1480, 1514, 1549, 1550
AflIII	ACRYGT	1 cut(s)	1302
AgsI	TTSAA	2 cut(s)	629, 1411
AhdI	GACNNNNNGTC	1 cut(s)	9
AjiI	CACGTC	1 cut(s)	411
AjnI	CCWGG	4 cut(s)	236, 279, 1189, 1398
AjuI	GAANNNNNNNTTGG	2 cut(s)	1316, 1348
AluBI	AGCT	9 cut(s)	34, 234, 548, 719, 724, 1079, 1380, 1456, 1673
AluI	AGCT	9 cut(s)	34, 234, 548, 719, 724, 1079, 1380, 1456, 1673
Alw21I	GWGCWC	1 cut(s)	36
Alw26I	GTCTC	1 cut(s)	289
AlwI	GGATC	1 cut(s)	248
AlwNI	CAGNNNCTG	1 cut(s)	548
Ama87I	CYCGRG	2 cut(s)	60, 854
Aor51HI	AGCGCT	1 cut(s)	1389
AoxI	GGCC	7 cut(s)	142, 508, 828, 1036, 1109, 1273, 1557
ApeKI	GCWGC	1 cut(s)	1385
ApoI	RAATTY	4 cut(s)	623, 920, 1242, 1358
Asp700I	GAANNNNTTC	1 cut(s)	1304
Asp718I	GGTACC	1 cut(s)	1130
AspLEI	GCGC	4 cut(s)	87, 435, 1291, 1390
AspS9I	GGNCC	4 cut(s)	598, 829, 1540, 1557
AsuHPI	GGTGA	1 cut(s)	913
AvaI	CYCGRG	2 cut(s)	60, 854
AvaII	GGWCC	2 cut(s)	598, 1540
AxyI	CCTNAGG	1 cut(s)	1230
BaeGI	GKGCMC	2 cut(s)	1064, 1471
BaeI	ACNNNNGTAYC	2 cut(s)	1468, 1501
BalI	TGGCCA	2 cut(s)	1111, 1275
BanI	GGYRCC	2 cut(s)	1130, 1288
BanII	GRGCYC	2 cut(s)	36, 61
BbsI	GAAGAC	1 cut(s)	265
Bbv12I	GWGCWC	1 cut(s)	36
BbvCI	CCTCAGC	1 cut(s)	501
BbvI	GCAGC	1 cut(s)	1397
BccI	CCATC	8 cut(s)	53, 209, 332, 937, 1024, 1143, 1279, 1548
BceAI	ACGGC	2 cut(s)	157, 1276
BciT130I	CCWGG	4 cut(s)	238, 281, 1191, 1400
BclI	TGATCA	1 cut(s)	1458
BcoDI	GTCTC	1 cut(s)	289
BfaI	CTAG	2 cut(s)	1148, 1377
BfmI	CTRYAG	2 cut(s)	549, 1154
BfoI	RGCGCY	3 cut(s)	436, 1292, 1391
BfuAI	ACCTGC	1 cut(s)	781
BglI	GCCNNNNNGGC	1 cut(s)	444
BglII	AGATCT	1 cut(s)	454
BisI	GCNGC	5 cut(s)	145, 436, 803, 1338, 1386
BlpI	GCTNAGC	3 cut(s)	720, 1341, 1452
BlsI	GCNGC	5 cut(s)	146, 437, 804, 1339, 1387
Bme1390I	CCNGG	4 cut(s)	238, 281, 1191, 1400
Bme18I	GGWCC	2 cut(s)	598, 1540
BmeRI	GACNNNNNGTC	1 cut(s)	9
BmeT110I	CYCGRG	2 cut(s)	60, 854
BmgBI	CACGTC	1 cut(s)	411
BmgT120I	GGNCC	4 cut(s)	598, 829, 1540, 1557
BmiI	GGNNCC	5 cut(s)	988, 1132, 1290, 1541, 1542
BmrFI	CCNGG	4 cut(s)	238, 281, 1191, 1400
BmrI	ACTGGG	1 cut(s)	489
BmsI	GCATC	1 cut(s)	1166
BmuI	ACTGGG	1 cut(s)	489
BpiI	GAAGAC	1 cut(s)	265
BplI	GAGNNNNNCTC	2 cut(s)	83, 115
BpmI	CTGGAG	2 cut(s)	220, 1002
Bpu10I	CCTNAGC	3 cut(s)	35, 501, 890
Bpu1102I	GCTNAGC	3 cut(s)	720, 1341, 1452
BpuEI	CTTGAG	2 cut(s)	858, 1205
BsaHI	GRCGYC	2 cut(s)	959, 1289
BsaI	GGTCTC	1 cut(s)	289
BsaJI	CCNNGG	5 cut(s)	24, 145, 439, 1190, 1435
BsaXI	ACNNNNNCTCC	6 cut(s)	58, 80, 88, 110, 380, 410
Bsc4I	CCNNNNNNNGG	7 cut(s)	414, 1140, 1315, 1480, 1514, 1549, 1550
Bse118I	RCCGGY	1 cut(s)	1473
Bse1I	ACTGG	3 cut(s)	484, 985, 1606
Bse21I	CCTNAGG	1 cut(s)	1230
Bse3DI	GCAATG	2 cut(s)	544, 1224
BseBI	CCWGG	4 cut(s)	238, 281, 1191, 1400
BseDI	CCNNGG	5 cut(s)	24, 145, 439, 1190, 1435
BseGI	GGATG	5 cut(s)	676, 972, 1035, 1290, 1559
BseLI	CCNNNNNNNGG	7 cut(s)	414, 1140, 1315, 1480, 1514, 1549, 1550
BseMI	GCAATG	2 cut(s)	544, 1224
BseMII	CTCAG	6 cut(s)	49, 134, 492, 690, 711, 921
BseNI	ACTGG	3 cut(s)	484, 985, 1606
BseRI	GAGGAG	3 cut(s)	88, 953, 1343
BseSI	GKGCMC	2 cut(s)	1064, 1471
BseX3I	CGGCCG	1 cut(s)	142
BseXI	GCAGC	1 cut(s)	1397
BsgI	GTGCAG	1 cut(s)	916
Bsh1236I	CGCG	4 cut(s)	87, 147, 171, 805
Bsh1285I	CGRYCG	5 cut(s)	145, 151, 175, 300, 850
BshFI	GGCC	7 cut(s)	144, 510, 830, 1038, 1111, 1275, 1559
BshNI	GGYRCC	2 cut(s)	1130, 1288
BsiEI	CGRYCG	5 cut(s)	145, 151, 175, 300, 850
BsiHKAI	GWGCWC	1 cut(s)	36
BsiHKCI	CYCGRG	2 cut(s)	60, 854
BsiSI	CCGG	1 cut(s)	1474
BslFI	GGGAC	3 cut(s)	162, 854, 1553
BslI	CCNNNNNNNGG	7 cut(s)	414, 1140, 1315, 1480, 1514, 1549, 1550
BsmAI	GTCTC	1 cut(s)	289
BsmFI	GGGAC	3 cut(s)	162, 854, 1553
BsmI	GAATGC	1 cut(s)	571
BsnI	GGCC	7 cut(s)	144, 510, 830, 1038, 1111, 1275, 1559
Bso31I	GGTCTC	1 cut(s)	289
BsoBI	CYCGRG	2 cut(s)	60, 854
Bsp1286I	GDGCHC	4 cut(s)	36, 61, 1064, 1471
Bsp143I	GATC	6 cut(s)	166, 240, 297, 454, 847, 1458
Bsp1720I	GCTNAGC	3 cut(s)	720, 1341, 1452
Bsp19I	CCATGG	1 cut(s)	24
Bsp68I	TCGCGA	1 cut(s)	171
BspACI	CCGC	6 cut(s)	29, 145, 147, 436, 803, 1337
BspANI	GGCC	7 cut(s)	144, 510, 830, 1038, 1111, 1275, 1559
BspCNI	CTCAG	6 cut(s)	48, 133, 493, 691, 712, 922
BspFNI	CGCG	4 cut(s)	87, 147, 171, 805
BspLI	GGNNCC	5 cut(s)	988, 1132, 1290, 1541, 1542
BspMAI	CTGCAG	1 cut(s)	1158
BspMI	ACCTGC	1 cut(s)	781
BspPI	GGATC	1 cut(s)	248
BspQI	GCTCTTC	1 cut(s)	1011
BspT107I	GGYRCC	2 cut(s)	1130, 1288
BspTNI	GGTCTC	1 cut(s)	289
BsrDI	GCAATG	2 cut(s)	544, 1224
BsrFI	RCCGGY	1 cut(s)	1473
BsrI	ACTGG	3 cut(s)	484, 985, 1606
BssAI	RCCGGY	1 cut(s)	1473
BssECI	CCNNGG	5 cut(s)	24, 145, 439, 1190, 1435
BssMI	GATC	6 cut(s)	166, 240, 297, 454, 847, 1458
BssNI	GRCGYC	2 cut(s)	959, 1289
BssT1I	CCWWGG	2 cut(s)	24, 439
Bst2UI	CCWGG	4 cut(s)	238, 281, 1191, 1400
Bst4CI	ACNGT	5 cut(s)	11, 176, 356, 748, 1119
Bst6I	CTCTTC	4 cut(s)	254, 354, 1011, 1065
BstACI	GRCGYC	2 cut(s)	959, 1289
BstC8I	GCNNGC	2 cut(s)	21, 1471
BstDSI	CCRYGG	3 cut(s)	24, 145, 1435
BstF5I	GGATG	5 cut(s)	676, 972, 1035, 1290, 1559
BstFNI	CGCG	4 cut(s)	87, 147, 171, 805
BstH2I	RGCGCY	3 cut(s)	436, 1292, 1391
BstHHI	GCGC	4 cut(s)	87, 435, 1291, 1390
BstKTI	GATC	6 cut(s)	169, 243, 300, 457, 850, 1461
BstMAI	GTCTC	1 cut(s)	289
BstMBI	GATC	6 cut(s)	166, 240, 297, 454, 847, 1458
BstMCI	CGRYCG	5 cut(s)	145, 151, 175, 300, 850
BstMWI	GCNNNNNNNGC	4 cut(s)	150, 444, 1085, 1565
BstNI	CCWGG	4 cut(s)	238, 281, 1191, 1400
BstNSI	RCATGY	3 cut(s)	1306, 1373, 1666
BstSCI	CCNGG	4 cut(s)	236, 279, 1189, 1398
BstSFI	CTRYAG	2 cut(s)	549, 1154
BstSLI	GKGCMC	2 cut(s)	1064, 1471
BstUI	CGCG	4 cut(s)	87, 147, 171, 805
BstV1I	GCAGC	1 cut(s)	1397
BstV2I	GAAGAC	1 cut(s)	265
BstX2I	RGATCY	2 cut(s)	240, 454
BstXI	CCANNNNNNTGG	1 cut(s)	52
BstYI	RGATCY	2 cut(s)	240, 454
BstZI	CGGCCG	1 cut(s)	142
Bsu36I	CCTNAGG	1 cut(s)	1230
BsuRI	GGCC	7 cut(s)	144, 510, 830, 1038, 1111, 1275, 1559
BtgI	CCRYGG	3 cut(s)	24, 145, 1435
BtgZI	GCGATG	1 cut(s)	1189
BtrI	CACGTC	1 cut(s)	411
BtsCI	GGATG	5 cut(s)	676, 972, 1035, 1290, 1559
BtsIMutI	CAGTG	2 cut(s)	352, 978
BtuMI	TCGCGA	1 cut(s)	171
BveI	ACCTGC	1 cut(s)	781
Cac8I	GCNNGC	2 cut(s)	21, 1471
CaiI	CAGNNNCTG	1 cut(s)	548
CfoI	GCGC	4 cut(s)	87, 435, 1291, 1390
Cfr10I	RCCGGY	1 cut(s)	1473
Cfr13I	GGNCC	4 cut(s)	598, 829, 1540, 1557
Cfr42I	CCGCGG	1 cut(s)	148
CseI	GACGC	1 cut(s)	948
Csp6I	GTAC	6 cut(s)	484, 677, 859, 1120, 1131, 1477
CviAII	CATG	9 cut(s)	25, 362, 497, 911, 1303, 1370, 1480, 1549, 1663
CviQI	GTAC	6 cut(s)	484, 677, 859, 1120, 1131, 1477
DinI	GGCGCC	1 cut(s)	1290
DpnI	GATC	6 cut(s)	168, 242, 299, 456, 849, 1460
DpnII	GATC	6 cut(s)	166, 240, 297, 454, 847, 1458
DriI	GACNNNNNGTC	1 cut(s)	9
EaeI	YGGCCR	3 cut(s)	142, 1109, 1273
EagI	CGGCCG	1 cut(s)	142
Eam1104I	CTCTTC	4 cut(s)	254, 354, 1011, 1065
Eam1105I	GACNNNNNGTC	1 cut(s)	9
EarI	CTCTTC	4 cut(s)	254, 354, 1011, 1065
EciI	GGCGGA	1 cut(s)	44
Ecl136II	GAGCTC	1 cut(s)	34
EclXI	CGGCCG	1 cut(s)	142
Eco130I	CCWWGG	2 cut(s)	24, 439
Eco147I	AGGCCT	1 cut(s)	1038
Eco24I	GRGCYC	2 cut(s)	36, 61
Eco31I	GGTCTC	1 cut(s)	289
Eco32I	GATATC	1 cut(s)	634
Eco47I	GGWCC	2 cut(s)	598, 1540
Eco47III	AGCGCT	1 cut(s)	1389
Eco52I	CGGCCG	1 cut(s)	142
Eco53kI	GAGCTC	1 cut(s)	34
Eco57I	CTGAAG	3 cut(s)	611, 686, 1239
Eco81I	CCTNAGG	1 cut(s)	1230
Eco88I	CYCGRG	2 cut(s)	60, 854
EcoICRI	GAGCTC	1 cut(s)	34
EcoO109I	RGGNCCY	1 cut(s)	1540
EcoRII	CCWGG	4 cut(s)	236, 279, 1189, 1398
EcoRV	GATATC	1 cut(s)	634
EcoT14I	CCWWGG	2 cut(s)	24, 439
EcoT38I	GRGCYC	2 cut(s)	36, 61
EgeI	GGCGCC	1 cut(s)	1290
EheI	GGCGCC	1 cut(s)	1290
ErhI	CCWWGG	2 cut(s)	24, 439
FaeI	CATG	9 cut(s)	28, 365, 500, 914, 1306, 1373, 1483, 1552, 1666
FaqI	GGGAC	3 cut(s)	162, 854, 1553
FatI	CATG	9 cut(s)	24, 361, 496, 910, 1302, 1369, 1479, 1548, 1662
FbaI	TGATCA	1 cut(s)	1458
Fnu4HI	GCNGC	5 cut(s)	145, 436, 803, 1338, 1386
FokI	GGATG	5 cut(s)	683, 979, 1042, 1297, 1566
FriOI	GRGCYC	2 cut(s)	36, 61
Fsp4HI	GCNGC	5 cut(s)	145, 436, 803, 1338, 1386
FspBI	CTAG	2 cut(s)	1148, 1377
GlaI	GCGC	4 cut(s)	86, 434, 1290, 1389
GluI	GCNGC	5 cut(s)	145, 436, 803, 1338, 1386
GsuI	CTGGAG	2 cut(s)	220, 1002
HaeII	RGCGCY	3 cut(s)	436, 1292, 1391
HaeIII	GGCC	7 cut(s)	144, 510, 830, 1038, 1111, 1275, 1559
HapII	CCGG	1 cut(s)	1474
HgaI	GACGC	1 cut(s)	948
HhaI	GCGC	4 cut(s)	87, 435, 1291, 1390
Hin1I	GRCGYC	2 cut(s)	959, 1289
Hin1II	CATG	9 cut(s)	28, 365, 500, 914, 1306, 1373, 1483, 1552, 1666
Hin6I	GCGC	4 cut(s)	85, 433, 1289, 1388
HinP1I	GCGC	4 cut(s)	85, 433, 1289, 1388
HincII	GTYRAC	1 cut(s)	646
HindII	GTYRAC	1 cut(s)	646
HindIII	AAGCTT	1 cut(s)	1671
HinfI	GANTC	7 cut(s)	95, 224, 289, 371, 391, 807, 852
HpaII	CCGG	1 cut(s)	1474
HphI	GGTGA	1 cut(s)	913
Hpy166II	GTNNAC	2 cut(s)	646, 708
Hpy188I	TCNGA	4 cut(s)	15, 108, 454, 1417
Hpy188III	TCNNGA	9 cut(s)	170, 286, 301, 689, 698, 875, 1257, 1363, 1426
Hpy8I	GTNNAC	2 cut(s)	646, 708
Hpy99I	CGWCG	8 cut(s)	92, 137, 140, 143, 224, 257, 266, 269
HpyAV	CCTTC	5 cut(s)	190, 387, 661, 1163, 1630
HpyCH4III	ACNGT	5 cut(s)	11, 176, 356, 748, 1119
HpyCH4IV	ACGT	1 cut(s)	410
HpyCH4V	TGCA	8 cut(s)	530, 537, 542, 770, 897, 1156, 1568, 1662
HpyF10VI	GCNNNNNNNGC	4 cut(s)	150, 444, 1085, 1565
HpySE526I	ACGT	1 cut(s)	410
Hsp92I	GRCGYC	2 cut(s)	959, 1289
Hsp92II	CATG	9 cut(s)	28, 365, 500, 914, 1306, 1373, 1483, 1552, 1666
HspAI	GCGC	4 cut(s)	85, 433, 1289, 1388
KasI	GGCGCC	1 cut(s)	1288
KflI	GGGWCCC	1 cut(s)	1540
KpnI	GGTACC	1 cut(s)	1134
Ksp22I	TGATCA	1 cut(s)	1458
KspI	CCGCGG	1 cut(s)	148
Kzo9I	GATC	6 cut(s)	166, 240, 297, 454, 847, 1458
LguI	GCTCTTC	1 cut(s)	1011
LmnI	GCTCC	2 cut(s)	31, 239
Lsp1109I	GCAGC	1 cut(s)	1397
LweI	GCATC	1 cut(s)	1166
MaeI	CTAG	2 cut(s)	1148, 1377
MaeII	ACGT	1 cut(s)	410
MaeIII	GTNAC	3 cut(s)	1318, 1372, 1579
MalI	GATC	6 cut(s)	168, 242, 299, 456, 849, 1460
MboI	GATC	6 cut(s)	166, 240, 297, 454, 847, 1458
MfeI	CAATTG	1 cut(s)	213
MflI	RGATCY	2 cut(s)	240, 454
MhlI	GDGCHC	4 cut(s)	36, 61, 1064, 1471
MlsI	TGGCCA	2 cut(s)	1111, 1275
MluCI	AATT	9 cut(s)	213, 525, 623, 681, 779, 904, 920, 1242, 1358
MluNI	TGGCCA	2 cut(s)	1111, 1275
Mly113I	GGCGCC	1 cut(s)	1289
MlyI	GAGTC	3 cut(s)	89, 218, 846
MmeI	TCCRAC	2 cut(s)	252, 1332
Mox20I	TGGCCA	2 cut(s)	1111, 1275
MroXI	GAANNNNTTC	1 cut(s)	1304
MscI	TGGCCA	2 cut(s)	1111, 1275
MseI	TTAA	1 cut(s)	918
MslI	CAYNNNNRTG	1 cut(s)	50
Msp20I	TGGCCA	2 cut(s)	1111, 1275
MspA1I	CMGCKG	1 cut(s)	147
MspI	CCGG	1 cut(s)	1474
MspR9I	CCNGG	4 cut(s)	238, 281, 1191, 1400
MunI	CAATTG	1 cut(s)	213
Mva1269I	GAATGC	1 cut(s)	571
MvaI	CCWGG	4 cut(s)	238, 281, 1191, 1400
MvnI	CGCG	4 cut(s)	87, 147, 171, 805
MwoI	GCNNNNNNNGC	4 cut(s)	150, 444, 1085, 1565
NarI	GGCGCC	1 cut(s)	1289
NcoI	CCATGG	1 cut(s)	24
NdeII	GATC	6 cut(s)	166, 240, 297, 454, 847, 1458
NlaIII	CATG	9 cut(s)	28, 365, 500, 914, 1306, 1373, 1483, 1552, 1666
NlaIV	GGNNCC	5 cut(s)	988, 1132, 1290, 1541, 1542
NmeAIII	GCCGAG	1 cut(s)	454
NmuCI	GTSAC	2 cut(s)	1372, 1579
NruI	TCGCGA	1 cut(s)	171
NspI	RCATGY	3 cut(s)	1306, 1373, 1666
PaeR7I	CTCGAG	1 cut(s)	60
PaqCI	CACCTGC	1 cut(s)	781
PceI	AGGCCT	1 cut(s)	1038
PciI	ACATGT	1 cut(s)	1302
PciSI	GCTCTTC	1 cut(s)	1011
PcsI	WCGNNNNNNNCGW	4 cut(s)	258, 261, 293, 685
PctI	GAATGC	1 cut(s)	571
PdmI	GAANNNNTTC	1 cut(s)	1304
PfeI	GAWTC	4 cut(s)	289, 371, 391, 807
PflFI	GACNNNGTC	1 cut(s)	866
PflMI	CCANNNNNTGG	1 cut(s)	414
PfoI	TCCNGGA	1 cut(s)	236
PkrI	GCNGC	5 cut(s)	146, 437, 804, 1339, 1387
Ple19I	CGATCG	2 cut(s)	300, 850
PleI	GAGTC	3 cut(s)	89, 218, 846
PluTI	GGCGCC	1 cut(s)	1292
PpsI	GAGTC	3 cut(s)	89, 218, 846
PpuMI	RGGWCCY	1 cut(s)	1540
PscI	ACATGT	1 cut(s)	1302
Psp124BI	GAGCTC	1 cut(s)	36
Psp5II	RGGWCCY	1 cut(s)	1540
Psp6I	CCWGG	4 cut(s)	236, 279, 1189, 1398
PspGI	CCWGG	4 cut(s)	236, 279, 1189, 1398
PspN4I	GGNNCC	5 cut(s)	988, 1132, 1290, 1541, 1542
PspPI	GGNCC	4 cut(s)	598, 829, 1540, 1557
PspPPI	RGGWCCY	1 cut(s)	1540
PspXI	VCTCGAGB	1 cut(s)	60
PsrI	GAACNNNNNNTAC	2 cut(s)	669, 701
PstI	CTGCAG	1 cut(s)	1158
PstNI	CAGNNNCTG	1 cut(s)	548
PsuI	RGATCY	2 cut(s)	240, 454
PsyI	GACNNNGTC	1 cut(s)	866
PvuI	CGATCG	2 cut(s)	300, 850
RruI	TCGCGA	1 cut(s)	171
RsaI	GTAC	6 cut(s)	485, 678, 860, 1121, 1132, 1478
RsaNI	GTAC	6 cut(s)	484, 677, 859, 1120, 1131, 1477
RseI	CAYNNNNRTG	1 cut(s)	50
SacI	GAGCTC	1 cut(s)	36
SacII	CCGCGG	1 cut(s)	148
SapI	GCTCTTC	1 cut(s)	1011
SaqAI	TTAA	1 cut(s)	918
SatI	GCNGC	5 cut(s)	145, 436, 803, 1338, 1386
Sau3AI	GATC	6 cut(s)	166, 240, 297, 454, 847, 1458
Sau96I	GGNCC	4 cut(s)	598, 829, 1540, 1557
SchI	GAGTC	3 cut(s)	89, 218, 846
ScrFI	CCNGG	4 cut(s)	238, 281, 1191, 1400
SduI	GDGCHC	4 cut(s)	36, 61, 1064, 1471
SfaNI	GCATC	1 cut(s)	1166
SfcI	CTRYAG	2 cut(s)	549, 1154
SfoI	GGCGCC	1 cut(s)	1290
Sfr274I	CTCGAG	1 cut(s)	60
Sfr303I	CCGCGG	1 cut(s)	148
SgrBI	CCGCGG	1 cut(s)	148
SinI	GGWCC	2 cut(s)	598, 1540
SlaI	CTCGAG	1 cut(s)	60
SmiMI	CAYNNNNRTG	1 cut(s)	50
SmlI	CTYRAG	3 cut(s)	60, 873, 1184
SmoI	CTYRAG	3 cut(s)	60, 873, 1184
Sse9I	AATT	9 cut(s)	213, 525, 623, 681, 779, 904, 920, 1242, 1358
SseBI	AGGCCT	1 cut(s)	1038
SsiI	CCGC	6 cut(s)	29, 145, 147, 436, 803, 1337
SspDI	GGCGCC	1 cut(s)	1288
SspMI	CTAG	2 cut(s)	1148, 1377
SstI	GAGCTC	1 cut(s)	36
StuI	AGGCCT	1 cut(s)	1038
StyD4I	CCNGG	4 cut(s)	236, 279, 1189, 1398
StyI	CCWWGG	2 cut(s)	24, 439
TaaI	ACNGT	5 cut(s)	11, 176, 356, 748, 1119
TaiI	ACGT	1 cut(s)	413
TaqI	TCGA	6 cut(s)	61, 114, 287, 846, 850, 1213
TasI	AATT	9 cut(s)	213, 525, 623, 681, 779, 904, 920, 1242, 1358
TauI	GCSGC	4 cut(s)	147, 438, 805, 1340
TfiI	GAWTC	4 cut(s)	289, 371, 391, 807
Tru1I	TTAA	1 cut(s)	918
Tru9I	TTAA	1 cut(s)	918
TscAI	CASTG	2 cut(s)	359, 985
TseFI	GTSAC	2 cut(s)	1372, 1579
TseI	GCWGC	1 cut(s)	1385
Tsp45I	GTSAC	2 cut(s)	1372, 1579
TspDTI	ATGAA	5 cut(s)	95, 485, 578, 643, 998
TspGWI	ACGGA	2 cut(s)	384, 1452
TspRI	CASTG	2 cut(s)	359, 985
Tth111I	GACNNNGTC	1 cut(s)	866
Van91I	CCANNNNNTGG	1 cut(s)	414
VpaK11BI	GGWCC	2 cut(s)	598, 1540
XapI	RAATTY	4 cut(s)	623, 920, 1242, 1358
XceI	RCATGY	3 cut(s)	1306, 1373, 1666
XcmI	CCANNNNNNNNNTGG	1 cut(s)	997
XhoI	CTCGAG	1 cut(s)	60
XmnI	GAANNNNTTC	1 cut(s)	1304
XspI	CTAG	2 cut(s)	1148, 1377

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.