Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0006527

Physical Location & Seq

Genomic Coordinates Reverse (-)

7153268 .. 7166813

Gene Structure 13546 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1102300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2796 bp

ATGGATTCGTACCAAGCGCAGCAGCGGTACCTGAGACCGCCCCCGCCGCCGCAACCACCGGTCACGTCGGATCCCTATCACTACCAGCAGCAGCAACAGCAACCAAGGCCAGAGTGGTACCCCAACCAGTACCAGTACCCACCACCTCCGCCGTACGCTGAACATCTCCCTCCCCCGGGTTCTTATCCTCCGCTCCCTCACCCTTATCCTGCGCAACCCCCAAATCACGCCCACTTCCCTCCTCCCCCTCCCCCTCCTCATCAATCTCGCCCTCACCTGCCTCCCCCTCCGCCTCGCCCTCCTCCACCTCATTCTTATCAGCACGCCCAGGAGTGGGGAGCTGCTCCTCCGAGTTGGCCGAATTACGCAGCCCCAAATAATCAAGATTGGGAAGCAAAGGCTAAGGCGTGGGCAGATGCCAGAACGGCAATGGAGACTCAACATCAACAGGTGCAGTTTCCACCGGCGGGGGGATTAGAAGAACAGAGTCACTATCAGGAACAGTATCCCCACAACGGTGACCCGCACTATTCTGGTAGTCACCATCAGTCATTTTCTGCATCTAGCTATCAACAGGTTCCAGATTCAGGTGCTCCAACACACTATCCACCGGGAATTCATTCCCAGGAGACTTCATCAATCGGCTCTGAACTATCTTCTTGTGCTCATCACGCTGTTAGAGATGGAAAGCCAGCTGGGGACTCAAATGCTCTGTTTCACCGTCAAGGAATCTTATCTACAAGTCCATCTGTCCATCAGCAGGAGGTACCTTATAGTTATTCTTCTGTTACAGGCAATGAAGGGACTGCAGCTCAAGAAGGACAACATCACATGCAGCCATCGCAGCCCTTTCCTTTTGCCTATGGCAGTCAGTCTGCTGATCCAACAACCAATCTTGCTGACCAGCCTTTAGAATTTGCACCTGGGTTTAGCTTTGATCATGGTCCACACATGCAGTCCAGCTATACTTATCATGATTCACATGGAACTATAAGAGGTGTCGACCCAGTGACCACAGCACCCTCCGTGAATACCTGGCCTAGTTCTGTTGCACCTGGTGTAGGTTATCCTCATAATGTTCCAGTTCCATCAGGACTACAGCATGATCCCTGCAATGCAATACCATCTCCTGTTCCTGGCCATTCTCCACATTCGTTTGGAAGTTTCCCATCTGCTGCAATGCCCTTTGGTCTCACTGCAGGAACCACAGTTCACTCTACTGCAGCCTTCCCTGGTGATGCATACGGAGTTTCTACAGTTACTGAGCGCCCCAAAAAGGCTCCAGTGCCTAGTTGGCTTAGAGACGAAATTAAGAAAGCAGTCATCACAAGTTCTTCTATGGACCATCCTAAGGAGGAAACTCAATCTTTTGAGGATGAAGGTTTTGACAGATCTTTTGGGAAAGGTGATCAAGCAGACAGCAAGAGCGTCGATTCTTCTAGACCAACAGAAGAAGAGGATGACGAGGATCAAGTGGAAGCTGCTAGGACTGCAGCAATTAACCAAGAAATAAAGCGTATCCTAACTGAAGTTTTCTTGAAGGTTACTGATGAGCTGTTTGATGAAATTGCTACAAAAGTTCTTACTGAAGATGATCTGACAGTTGAAGTGGAACAGAGCGCACTCATTTCAAACCATAAGGTTTTGCCATCTCCACCAGCAGTTACAACTCCCAAGGCTTCTGCAAAAGTTCTAATTGCGGCCAAGTCTAAGGAATATGAGACTGGAGATGTAAAATCCAGTTCTACTTTACCTGGTGATGTATTGGGTCTTGCAAATTATGCTACAGATGACGAAGATGGAGATAGTGATATTCAGAGTTCTGGTGTGCCAAATTCTGGGAAAGATGCTAAGCTTCAGCAGTTAACTGTCAGAACGCCTTCAAATGATAGGCATAATGCCACTGTAAATGACCGTTCTTCTGTAGAGCTTCAAGAGCATAGTAAAAGTCAGACAATTTTGGAGAGTGGACGGGGCAAAACCAGTTCACTTGAGTCTAATAATAGAAACAAGAATGATGATAGTAATGGTGATAGAATTTTGCCAGATGGAACTACTGCCTCTAGGTTGAAGGATACTGTAGGGATATTCAAACCTGAACTACCTAAAGAAACTGTCAATGTGAAAAACACATCAAAAGATGACCCACAAGTTATGGAGTCCAGGATGAAACCTGATAAGCATGATCGAGATGAGAATAAAAAGAGTTATGTAAAAGATATCAATAAGGAGATAGAAAGTGGTAAGATTAGGACAGATGAGAAGGGTGACGAGAATCATAAGGGACATTATCCGAGAAAGGAAAGGACATATGATCGGAATGGCTCAAAAGAAAAGGTGAAAGAGCAAAGTGTTAAGGAATCTGAGTCAAGAAAAAGGTCTTCCCATGCTGATGCCAAGGAGGGCAGAAGAGAAACAGAGCAACTCCATAGGGCTAATGCTAAAGAAGACAAGGACAGGAAAAGAGGATGGACAAAGGAAAAGGAGGGTGATAGATCAAGACATAAACATTCTACTGACTCAAGCAGGCACAAGGGAAGGCGCTCCTCTTCTGTTGGCAGTAGAGGAAGAAACAGCAAGGATGATTCAAGCGATGAAGCTTCTGATGATTCTAAAAGGAAGCGCCATTCAAGGAGATGTAACTTATCACCATCACCCGTCAGGTCCAGGAGAAGACAAGTTTTGCGGTCTCCACATAGCAAGCATTCTCAGCGCAGGCATTCTCCTTATTCTTCTCTTGAGACGAGCAGGGGAAGAAGGTCAAGATCCAGATCACCTGTGCGGCGGCAGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006464 GO:0006486 GO:0006807 GO:0008150 GO:0008152 GO:0008194 GO:0008378 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0012505 GO:0016740 GO:0016757 GO:0016758 GO:0019538 GO:0034645 GO:0035250 GO:0036211 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0070085 GO:0071554 GO:0071704 GO:0071840 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4228 Nucleotid_trans PNISR

Protein Analysis

932

Amino Acids

103.24

Weight (kDa)

6.82

Isoelectric Point (pI)

73.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PNISR	PF15996	444 - 530	6.4e-06	Arginine/serine-rich protein PNISR

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006527)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70620 AT1G70620 AT1G70620 AT1G70620 AT1G70620 AT1G70620 AT1G70620 AT1G70620 AT1G70620
fragaria_vesca	FvH4_4g25541
malus_domestica	MD13G1101700.v1.1 MD16G1102300.v1.1
prunus_persica	Prupe.1G244100_v2.0.a1 Prupe.1G244100_v2.0.a1 Prupe.1G244100_v2.0.a1 Prupe.1G244100_v2.0.a1
pyrus_communis	pycom13g08880 pycom16g08690
rosa_chinensis	RchiOBHm_Chr4g0432951
rosa_multiflora	Rmu_sc0001762.1_g000045
rosa_rugosa	Rorug04G0264300
rosa_samantha	Rh4AG318100 Rh4BG326200 Rh4CG341500 Rh4DG321400
rosa_wichuraiana	Rw4G027660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	285
Acc16I	TGCGCA	1 cut(s)	213
Acc36I	ACCTGC	1 cut(s)	285
Acc65I	GGTACC	3 cut(s)	27, 117, 766
AccB1I	GGYRCC	3 cut(s)	27, 117, 766
AccB7I	CCANNNNNTGG	1 cut(s)	1838
AccBSI	CCGCTC	1 cut(s)	193
AccI	GTMKAC	1 cut(s)	1002
AclWI	GGATC	6 cut(s)	65, 78, 875, 1100, 1476, 2760
AcoI	YGGCCR	3 cut(s)	356, 1138, 1701
AcsI	RAATTY	4 cut(s)	615, 914, 1834, 2037
AcuI	CTGAAG	3 cut(s)	1548, 1608, 1841
AdeI	CACNNNGTG	1 cut(s)	1058
AfaI	GTAC	7 cut(s)	11, 29, 119, 131, 137, 155, 768
AgeI	ACCGGT	1 cut(s)	58
AgsI	TTSAA	9 cut(s)	1540, 1607, 1632, 1884, 1934, 2071, 2092, 2589, 2631
AjiI	CACGTC	1 cut(s)	66
AleI	CACNNNNGTG	1 cut(s)	516
AloI	GAACNNNNNNTCC	2 cut(s)	1194, 1226
Alw21I	GWGCWC	2 cut(s)	595, 667
Alw26I	GTCTC	8 cut(s)	28, 428, 623, 1196, 1296, 1715, 2694, 2735
AlwI	GGATC	6 cut(s)	65, 78, 875, 1100, 1476, 2760
AlwNI	CAGNNNCTG	1 cut(s)	1262
Ama87I	CYCGRG	1 cut(s)	175
AoxI	GGCC	5 cut(s)	107, 356, 1037, 1138, 1701
ApoI	RAATTY	4 cut(s)	615, 914, 1834, 2037
ArsI	GACNNNNNNTTYG	2 cut(s)	1379, 1411
AsiGI	ACCGGT	1 cut(s)	58
Asp700I	GAANNNNTTC	2 cut(s)	619, 1879
Asp718I	GGTACC	3 cut(s)	27, 117, 766
AspLEI	GCGC	7 cut(s)	19, 214, 1269, 1622, 2544, 2625, 2715
AspS9I	GGNCC	3 cut(s)	944, 1342, 2664
AsuC2I	CCSGG	3 cut(s)	176, 177, 612
AvaI	CYCGRG	1 cut(s)	175
AvaII	GGWCC	3 cut(s)	944, 1342, 2664
AxyI	CCTNAGG	1 cut(s)	1350
BalI	TGGCCA	1 cut(s)	1140
BamHI	GGATCC	1 cut(s)	70
BanI	GGYRCC	3 cut(s)	27, 117, 766
BbsI	GAAGAC	3 cut(s)	2373, 2454, 2680
Bbv12I	GWGCWC	2 cut(s)	595, 667
BceAI	ACGGC	2 cut(s)	136, 441
BcgI	CGANNNNNNTGC	2 cut(s)	1411, 1445
BciVI	GTATCC	3 cut(s)	516, 1529, 2068
BclI	TGATCA	2 cut(s)	937, 1408
BcnI	CCSGG	3 cut(s)	176, 177, 612
BcoDI	GTCTC	8 cut(s)	28, 428, 623, 1196, 1296, 1715, 2694, 2735
BfaI	CTAG	6 cut(s)	564, 1041, 1290, 1440, 1485, 2064
BfmI	CTRYAG	9 cut(s)	807, 1097, 1197, 1221, 1254, 1491, 1785, 1923, 2079
BfoI	RGCGCY	3 cut(s)	1270, 2545, 2626
BfuAI	ACCTGC	1 cut(s)	285
BfuI	GTATCC	3 cut(s)	516, 1529, 2068
BglI	GCCNNNNNGGC	2 cut(s)	425, 1294
BglII	AGATCT	1 cut(s)	1391
BlpI	GCTNAGC	1 cut(s)	1851
Bme18I	GGWCC	3 cut(s)	944, 1342, 2664
BmeT110I	CYCGRG	1 cut(s)	175
BmgBI	CACGTC	1 cut(s)	66
BmgT120I	GGNCC	3 cut(s)	944, 1342, 2664
BmiI	GGNNCC	7 cut(s)	29, 72, 119, 579, 768, 1204, 1281
BmrI	ACTGGG	1 cut(s)	1001
BmsI	GCATC	5 cut(s)	406, 569, 1228, 1837, 2383
BmuI	ACTGGG	1 cut(s)	1001
BpiI	GAAGAC	3 cut(s)	2373, 2454, 2680
BpmI	CTGGAG	2 cut(s)	1266, 1746
Bpu10I	CCTNAGC	1 cut(s)	402
Bpu1102I	GCTNAGC	1 cut(s)	1851
BpuEI	CTTGAG	4 cut(s)	798, 2012, 2506, 2759
BpuMI	CCSGG	3 cut(s)	176, 177, 612
BsaBI	GATNNNNATC	2 cut(s)	75, 2770
BsaI	GGTCTC	3 cut(s)	28, 1196, 2694
BsaJI	CCNNGG	9 cut(s)	104, 174, 175, 327, 624, 923, 1231, 1674, 2397
BsaWI	WCCGGW	1 cut(s)	58
Bse118I	RCCGGY	2 cut(s)	58, 463
Bse1I	ACTGG	8 cut(s)	127, 133, 1007, 1082, 1283, 1729, 1740, 1983
Bse21I	CCTNAGG	1 cut(s)	1350
Bse3DI	GCAATG	4 cut(s)	435, 802, 1120, 1185
Bse8I	GATNNNNATC	2 cut(s)	75, 2770
BseDI	CCNNGG	9 cut(s)	104, 174, 175, 327, 624, 923, 1231, 1674, 2397
BseGI	GGATG	6 cut(s)	1345, 1381, 1465, 2172, 2474, 2587
BseJI	GATNNNNATC	2 cut(s)	75, 2770
BseMI	GCAATG	4 cut(s)	435, 802, 1120, 1185
BseMII	CTCAG	4 cut(s)	23, 1254, 2355, 2723
BseNI	ACTGG	8 cut(s)	127, 133, 1007, 1082, 1283, 1729, 1740, 1983
BseRI	GAGGAG	5 cut(s)	231, 246, 291, 336, 2536
BseYI	CCCAGC	1 cut(s)	695
BsgI	GTGCAG	1 cut(s)	473
BshFI	GGCC	5 cut(s)	109, 358, 1039, 1140, 1703
BshNI	GGYRCC	3 cut(s)	27, 117, 766
BshTI	ACCGGT	1 cut(s)	58
BsiHKAI	GWGCWC	2 cut(s)	595, 667
BsiHKCI	CYCGRG	1 cut(s)	175
BsiSI	CCGG	4 cut(s)	59, 176, 464, 611
BsiWI	CGTACG	1 cut(s)	153
BslFI	GGGAC	3 cut(s)	713, 817, 2298
BsmAI	GTCTC	8 cut(s)	28, 428, 623, 1196, 1296, 1715, 2694, 2735
BsmBI	CGTCTC	2 cut(s)	1296, 2735
BsmFI	GGGAC	3 cut(s)	713, 817, 2298
BsmI	GAATGC	2 cut(s)	2704, 2719
BsnI	GGCC	5 cut(s)	109, 358, 1039, 1140, 1703
Bso31I	GGTCTC	3 cut(s)	28, 1196, 2694
BsoBI	CYCGRG	1 cut(s)	175
Bsp1286I	GDGCHC	2 cut(s)	595, 667
Bsp1720I	GCTNAGC	1 cut(s)	1851
BspANI	GGCC	5 cut(s)	109, 358, 1039, 1140, 1703
BspCNI	CTCAG	4 cut(s)	24, 1255, 2356, 2722
BspHI	TCATGA	1 cut(s)	973
BspLI	GGNNCC	7 cut(s)	29, 72, 119, 579, 768, 1204, 1281
BspMAI	CTGCAG	4 cut(s)	811, 1201, 1225, 1495
BspMI	ACCTGC	1 cut(s)	285
BspPI	GGATC	6 cut(s)	65, 78, 875, 1100, 1476, 2760
BspT107I	GGYRCC	3 cut(s)	27, 117, 766
BspTNI	GGTCTC	3 cut(s)	28, 1196, 2694
BsrBI	CCGCTC	1 cut(s)	193
BsrDI	GCAATG	4 cut(s)	435, 802, 1120, 1185
BsrFI	RCCGGY	2 cut(s)	58, 463
BsrI	ACTGG	8 cut(s)	127, 133, 1007, 1082, 1283, 1729, 1740, 1983
BssAI	RCCGGY	2 cut(s)	58, 463
BssECI	CCNNGG	9 cut(s)	104, 174, 175, 327, 624, 923, 1231, 1674, 2397
BssT1I	CCWWGG	3 cut(s)	104, 1674, 2397
Bst6I	CTCTTC	3 cut(s)	1449, 2404, 2554
BstAPI	GCANNNNNTGC	1 cut(s)	1781
BstC8I	GCNNGC	5 cut(s)	324, 693, 2528, 2702, 2717
BstDEI	CTNAG	9 cut(s)	32, 402, 1263, 1298, 1350, 1710, 1851, 2364, 2709
BstEII	GGTNACC	1 cut(s)	518
BstENI	CCTNNNNNAGG	1 cut(s)	1059
BstF5I	GGATG	6 cut(s)	1345, 1381, 1465, 2172, 2474, 2587
BstH2I	RGCGCY	3 cut(s)	1270, 2545, 2626
BstHHI	GCGC	7 cut(s)	19, 214, 1269, 1622, 2544, 2625, 2715
BstMAI	GTCTC	8 cut(s)	28, 428, 623, 1196, 1296, 1715, 2694, 2735
BstNSI	RCATGY	2 cut(s)	835, 955
BstPI	GGTNACC	1 cut(s)	518
BstSFI	CTRYAG	9 cut(s)	807, 1097, 1197, 1221, 1254, 1491, 1785, 1923, 2079
BstV2I	GAAGAC	3 cut(s)	2373, 2454, 2680
BstX2I	RGATCY	3 cut(s)	70, 1391, 2765
BstYI	RGATCY	3 cut(s)	70, 1391, 2765
Bsu36I	CCTNAGG	1 cut(s)	1350
BsuI	GTATCC	3 cut(s)	516, 1529, 2068
BsuRI	GGCC	5 cut(s)	109, 358, 1039, 1140, 1703
BtgZI	GCGATG	2 cut(s)	825, 2607
BtrI	CACGTC	1 cut(s)	66
BtsCI	GGATG	6 cut(s)	1345, 1381, 1465, 2172, 2474, 2587
BtsI	GCAGTG	1 cut(s)	1194
BtsIMutI	CAGTG	4 cut(s)	1014, 1194, 1290, 1902
BveI	ACCTGC	1 cut(s)	285
Cac8I	GCNNGC	5 cut(s)	324, 693, 2528, 2702, 2717
CaiI	CAGNNNCTG	1 cut(s)	1262
CciI	TCATGA	1 cut(s)	973
CfoI	GCGC	7 cut(s)	19, 214, 1269, 1622, 2544, 2625, 2715
Cfr10I	RCCGGY	2 cut(s)	58, 463
Cfr13I	GGNCC	3 cut(s)	944, 1342, 2664
Cfr9I	CCCGGG	1 cut(s)	175
CseI	GACGC	1 cut(s)	1417
CsiI	ACCWGGT	2 cut(s)	1054, 1753
Csp6I	GTAC	7 cut(s)	10, 28, 118, 130, 136, 154, 767
CspAI	ACCGGT	1 cut(s)	58
CviAII	CATG	8 cut(s)	832, 941, 952, 974, 983, 1103, 2183, 2387
CviQI	GTAC	7 cut(s)	10, 28, 118, 130, 136, 154, 767
DdeI	CTNAG	9 cut(s)	32, 402, 1263, 1298, 1350, 1710, 1851, 2364, 2709
DraIII	CACNNNGTG	1 cut(s)	1058
EaeI	YGGCCR	3 cut(s)	356, 1138, 1701
Eam1104I	CTCTTC	3 cut(s)	1449, 2404, 2554
EarI	CTCTTC	3 cut(s)	1449, 2404, 2554
EciI	GGCGGA	2 cut(s)	138, 279
Eco130I	CCWWGG	3 cut(s)	104, 1674, 2397
Eco31I	GGTCTC	3 cut(s)	28, 1196, 2694
Eco32I	GATATC	1 cut(s)	2221
Eco47I	GGWCC	3 cut(s)	944, 1342, 2664
Eco57I	CTGAAG	3 cut(s)	1548, 1608, 1841
Eco81I	CCTNAGG	1 cut(s)	1350
Eco88I	CYCGRG	1 cut(s)	175
Eco91I	GGTNACC	1 cut(s)	518
EcoNI	CCTNNNNNAGG	1 cut(s)	1059
EcoO65I	GGTNACC	1 cut(s)	518
EcoRI	GAATTC	1 cut(s)	615
EcoRV	GATATC	1 cut(s)	2221
EcoT14I	CCWWGG	3 cut(s)	104, 1674, 2397
EcoT22I	ATGCAT	1 cut(s)	1243
ErhI	CCWWGG	3 cut(s)	104, 1674, 2397
Esp3I	CGTCTC	2 cut(s)	1296, 2735
FaeI	CATG	8 cut(s)	835, 944, 955, 977, 986, 1106, 2186, 2390
FaqI	GGGAC	3 cut(s)	713, 817, 2298
FatI	CATG	8 cut(s)	831, 940, 951, 973, 982, 1102, 2182, 2386
FauI	CCCGC	3 cut(s)	51, 460, 531
FauNDI	CATATG	1 cut(s)	2311
FbaI	TGATCA	2 cut(s)	937, 1408
FblI	GTMKAC	1 cut(s)	1002
FokI	GGATG	6 cut(s)	1332, 1388, 1472, 2179, 2481, 2594
FspBI	CTAG	6 cut(s)	564, 1041, 1290, 1440, 1485, 2064
FspI	TGCGCA	1 cut(s)	213
GlaI	GCGC	7 cut(s)	18, 213, 1268, 1621, 2543, 2624, 2714
GsaI	CCCAGC	1 cut(s)	699
GsuI	CTGGAG	2 cut(s)	1266, 1746
HaeII	RGCGCY	3 cut(s)	1270, 2545, 2626
HaeIII	GGCC	5 cut(s)	109, 358, 1039, 1140, 1703
HapII	CCGG	4 cut(s)	59, 176, 464, 611
HgaI	GACGC	1 cut(s)	1417
HhaI	GCGC	7 cut(s)	19, 214, 1269, 1622, 2544, 2625, 2715
Hin1II	CATG	8 cut(s)	835, 944, 955, 977, 986, 1106, 2186, 2390
Hin6I	GCGC	7 cut(s)	17, 212, 1267, 1620, 2542, 2623, 2713
HinP1I	GCGC	7 cut(s)	17, 212, 1267, 1620, 2542, 2623, 2713
HincII	GTYRAC	2 cut(s)	1003, 1866
HindII	GTYRAC	2 cut(s)	1003, 1866
HindIII	AAGCTT	2 cut(s)	1853, 2598
HpaI	GTTAAC	1 cut(s)	1866
HpaII	CCGG	4 cut(s)	59, 176, 464, 611
Hpy166II	GTNNAC	6 cut(s)	947, 1003, 1213, 1866, 1970, 1988
Hpy8I	GTNNAC	6 cut(s)	947, 1003, 1213, 1866, 1970, 1988
Hpy99I	CGWCG	2 cut(s)	70, 1433
HpyCH4IV	ACGT	1 cut(s)	65
HpyF3I	CTNAG	9 cut(s)	32, 402, 1263, 1298, 1350, 1710, 1851, 2364, 2709
HpySE526I	ACGT	1 cut(s)	65
Hsp92II	CATG	8 cut(s)	835, 944, 955, 977, 986, 1106, 2186, 2390
HspAI	GCGC	7 cut(s)	17, 212, 1267, 1620, 2542, 2623, 2713
KpnI	GGTACC	3 cut(s)	31, 121, 770
Ksp22I	TGATCA	2 cut(s)	937, 1408
KspAI	GTTAAC	1 cut(s)	1866
LmnI	GCTCC	6 cut(s)	198, 338, 349, 598, 1285, 2549
LweI	GCATC	5 cut(s)	406, 569, 1228, 1837, 2383
MabI	ACCWGGT	2 cut(s)	1054, 1753
MaeI	CTAG	6 cut(s)	564, 1041, 1290, 1440, 1485, 2064
MaeII	ACGT	1 cut(s)	65
MbiI	CCGCTC	1 cut(s)	193
MflI	RGATCY	3 cut(s)	70, 1391, 2765
MhlI	GDGCHC	2 cut(s)	595, 667
MlsI	TGGCCA	1 cut(s)	1140
MluNI	TGGCCA	1 cut(s)	1140
MlyI	GAGTC	7 cut(s)	430, 496, 695, 2003, 2168, 2375, 2513
MmeI	TCCRAC	3 cut(s)	48, 620, 908
Mox20I	TGGCCA	1 cut(s)	1140
Mph1103I	ATGCAT	1 cut(s)	1243
MroXI	GAANNNNTTC	2 cut(s)	619, 1879
MscI	TGGCCA	1 cut(s)	1140
MseI	TTAA	4 cut(s)	1311, 1500, 1865, 2355
MslI	CAYNNNNRTG	2 cut(s)	516, 2391
Msp20I	TGGCCA	1 cut(s)	1140
MspA1I	CMGCKG	2 cut(s)	25, 695
MspI	CCGG	4 cut(s)	59, 176, 464, 611
Mva1269I	GAATGC	2 cut(s)	2704, 2719
NciI	CCSGG	3 cut(s)	176, 177, 612
NdeI	CATATG	1 cut(s)	2311
NlaIII	CATG	8 cut(s)	835, 944, 955, 977, 986, 1106, 2186, 2390
NlaIV	GGNNCC	7 cut(s)	29, 72, 119, 579, 768, 1204, 1281
NmuCI	GTSAC	6 cut(s)	61, 488, 518, 539, 1009, 2267
NsbI	TGCGCA	1 cut(s)	213
NsiI	ATGCAT	1 cut(s)	1243
NspI	RCATGY	2 cut(s)	835, 955
OliI	CACNNNNGTG	1 cut(s)	516
PagI	TCATGA	1 cut(s)	973
PaqCI	CACCTGC	1 cut(s)	285
PctI	GAATGC	2 cut(s)	2704, 2719
PdmI	GAANNNNTTC	2 cut(s)	619, 1879
PfeI	GAWTC	9 cut(s)	5, 584, 729, 977, 1433, 2275, 2360, 2585, 2609
Pfl23II	CGTACG	1 cut(s)	153
PflMI	CCANNNNNTGG	1 cut(s)	1838
PfoI	TCCNGGA	2 cut(s)	2162, 2666
PinAI	ACCGGT	1 cut(s)	58
PleI	GAGTC	7 cut(s)	430, 495, 695, 2002, 2167, 2374, 2513
PpsI	GAGTC	7 cut(s)	430, 495, 695, 2002, 2167, 2374, 2513
PspEI	GGTNACC	1 cut(s)	518
PspFI	CCCAGC	1 cut(s)	695
PspLI	CGTACG	1 cut(s)	153
PspN4I	GGNNCC	7 cut(s)	29, 72, 119, 579, 768, 1204, 1281
PspPI	GGNCC	3 cut(s)	944, 1342, 2664
PstI	CTGCAG	4 cut(s)	811, 1201, 1225, 1495
PstNI	CAGNNNCTG	1 cut(s)	1262
PsuI	RGATCY	3 cut(s)	70, 1391, 2765
PvuII	CAGCTG	1 cut(s)	695
RsaI	GTAC	7 cut(s)	11, 29, 119, 131, 137, 155, 768
RsaNI	GTAC	7 cut(s)	10, 28, 118, 130, 136, 154, 767
RseI	CAYNNNNRTG	2 cut(s)	516, 2391
SalI	GTCGAC	1 cut(s)	1001
SaqAI	TTAA	4 cut(s)	1311, 1500, 1865, 2355
Sau96I	GGNCC	3 cut(s)	944, 1342, 2664
SchI	GAGTC	7 cut(s)	430, 496, 695, 2003, 2168, 2375, 2513
SduI	GDGCHC	2 cut(s)	595, 667
SexAI	ACCWGGT	2 cut(s)	1054, 1753
SfaNI	GCATC	5 cut(s)	406, 569, 1228, 1837, 2383
SfcI	CTRYAG	9 cut(s)	807, 1097, 1197, 1221, 1254, 1491, 1785, 1923, 2079
SgrAI	CRCCGGYG	1 cut(s)	463
SinI	GGWCC	3 cut(s)	944, 1342, 2664
SmaI	CCCGGG	1 cut(s)	177
SmiMI	CAYNNNNRTG	2 cut(s)	516, 2391
SmlI	CTYRAG	4 cut(s)	813, 1991, 2521, 2738
SmoI	CTYRAG	4 cut(s)	813, 1991, 2521, 2738
SspMI	CTAG	6 cut(s)	564, 1041, 1290, 1440, 1485, 2064
StyI	CCWWGG	3 cut(s)	104, 1674, 2397
TaiI	ACGT	1 cut(s)	68
TaqI	TCGA	3 cut(s)	1002, 1431, 2188
TauI	GCSGC	5 cut(s)	49, 52, 1703, 2785, 2788
TfiI	GAWTC	9 cut(s)	5, 584, 729, 977, 1433, 2275, 2360, 2585, 2609
Tru1I	TTAA	4 cut(s)	1311, 1500, 1865, 2355
Tru9I	TTAA	4 cut(s)	1311, 1500, 1865, 2355
TscAI	CASTG	4 cut(s)	1014, 1201, 1290, 1909
TseFI	GTSAC	6 cut(s)	61, 488, 518, 539, 1009, 2267
Tsp45I	GTSAC	6 cut(s)	61, 488, 518, 539, 1009, 2267
TspDTI	ATGAA	7 cut(s)	608, 624, 813, 1392, 1578, 2183, 2610
TspGWI	ACGGA	2 cut(s)	1015, 1260
TspMI	CCCGGG	1 cut(s)	175
TspRI	CASTG	4 cut(s)	1014, 1201, 1290, 1909
Van91I	CCANNNNNTGG	1 cut(s)	1838
VpaK11BI	GGWCC	3 cut(s)	944, 1342, 2664
XagI	CCTNNNNNAGG	1 cut(s)	1059
XapI	RAATTY	4 cut(s)	615, 914, 1834, 2037
XbaI	TCTAGA	1 cut(s)	1439
XceI	RCATGY	2 cut(s)	835, 955
XcmI	CCANNNNNNNNNTGG	3 cut(s)	111, 427, 1290
XmaI	CCCGGG	1 cut(s)	175
XmiI	GTMKAC	1 cut(s)	1002
XmnI	GAANNNNTTC	2 cut(s)	619, 1879
XspI	CTAG	6 cut(s)	564, 1041, 1290, 1440, 1485, 2064
Zsp2I	ATGCAT	1 cut(s)	1243

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.