Rw4G027660

Nucleotide-diphospho-sugar transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0006527
Physical Location & Seq
Forward (+)
54236542 .. 54246103
9562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G027660.1

Sequence Viewer

Length: 4812 bp
ATGGATTCTTACAGACCGCACCAGCAGCAGCAGTACATGAGGCCACCGCCACCTGCCACGTCGGATCCGTACCTCTACCAACAGCAGCAACCACCGCCTCCGCCACCGCCAAGACCACCACTCCCTCAGCAGCAGCAGCAGCAAGGCGCGTGGTACTCGAACCAGAACCAGTACCAATACCACTCATTACAGTCCCCGTCGCCTCCTCCGCAACAGTGGGGTCCGCCGCCTCATGTCGACCATCTGCCTCCCCCGCCGGGCTCTTATCCTCCGCCACCGCCTCCTCCTTATTCTGCGCACCACTATCGCCCTCACGGACCTCCTCAGTCTTATCATCCTCAGGAGTGGGCAGCTCAGAGTCGGCCGAATAACCAAGGTTGGGAATACCCAGCCCATAACAATGAGCAAGATTGGGCAGCCAAGGCTAGGGCATGGGCAGCTAGGACGGCAATGGAGAACCAGGATCAGCAATCACAGTTTACTCCAGCCGGCAGACCAGAAGAACACTCTCATTATCATGAGCAGTATCCTCAAAGTCAGCACCAGTCTCTTTCTGCATCTAGCTATCAACAGGTTTCAGTTTCAGGGACACCGGCACACCGGCCACCAGGAGTTCATCCCCAGGACACTAGCTATATCGGCTCCGAACCGTCTTCTTATGGACATCATGCTGTCAGATCTGCAATTCCATCTGTTCATCAGCAGGAGGTACCTTCTAGTTATTCTTCTGTTACAGGCAATGACGCTTCTGCAGAACTGAATGAGCACTCTTACAACTTCTTACCTATGCCAAATTCCTCAGGTCAGGAAGGACAGCACCATATGCAACAATCCCATGCAATACCATTTGGATATGGCAGTCATTCTGCAGATCCAGCAGCCAATCTTGCTGACCAGCCTTTAGAATTTGCATCTGGGTTTAATTCTGATCATGGTGCTCACGCATCCAGCTATCCTTATCATGATTCAGGTGGAAGTATAAGAGGTGTGGACCCTGCTACAACAGTTCCTTCCATGAATTCTTGGACTGTTTCTGTTGCACCTGGTGTGGTATATCCTCCTACCCTTCCAGTTCTACCATCAGGACCACAGCATGATCCTTCTATGACGATACCCTCTCCGGTCCCTGGAAATGCTCCACATTCATTTGGTAGTTTTCCTGGATTGAGCCTCCAGCCAACAATTCCAACCACTGGTCCTCCCTTTGCTCTCACTGCAGGAACTGCTGGTCATCCTACTGTGGCTTTCCCTGGTGATGCATACGGAGTTTCTACAATTTCTGAGCGCCCAAAAAAGGCTGCAGTGCCTAATTGGCTTAGAGAGGAAATTAAGAAAGTAAGCATCACAAATTCCACCATGGACCATCTCAAGGAGGAAACACAGTCTGTTGAGGATGAAGGTGTTGACAGATCTTTTGGGAAGGGTGATCAAGCCGATAGCAAAAGCATTGATTCTTCTAGATCAACTGAAGAAGAGGATGATGAGGATGAGGTGGAGGCAGCTCGGACTGCAGCAATTAACAAGGAAATAAAGCGTATCCTAACTGAAGTTCTCTCAACGGTTACTGATGAGCTCTTTGATGAAATTGCAACAAAAGTTCTTAGTGAAGATGATCTAACATCTGAAGTTGGACAGGGCAGACTCCCATCAAACCATAAGGTATTGCCATCTACCCCAGCCGTTTCAGATGCCAAGGCTTCTGCTAAGCTTCTAATTCCAGTCAAGACTAGGGAATCTGAGACTGAAGATGTCGGTGAAAAATCTAGCTCCAGTACACCTGGTGATATATTGGGTCTTGCAAATTATGCTACTGATGAAGATGACGATGATGATGATGGAGGTGGTGAAATTCCGAGTTCTGGTGTGCCAAAATCTGGGAAAGATCAGTTTACTGTTAGAAAGCCTCATGAGTCGCGTGATGCCACTGCAAATGGCAGTTCTCCAGTAGAGCTTGAAGAGCACAGGAGAAGTCAGACAAGTTTGCCGGATGATCTGAGCAAGAGTGATATTAGTTATAATAGAAACAAGAGTGAGGAAAGTAATGTTGATAAATATCTGCAAGATGGGACTACTGTGGAACCTGAACTATCTACAGAAAATGTTAATGTCAAAAAGACAGCCAAAAATGACCCACAAAGTAGGGAGTCCAGGATAAAGCCAGATAAGCATGATCAACATGAGAGTAAAAGGAGTTCTTTGAAAGAAAATAAGGAGGTAGACAGTGGTAAGATTAGGACAGACGAGAAGGGTGATGAGAATCGTAGGAGGCAGGATGAGAGGCCTCTGAGAAAGGAAAGGACAGATGATCGGAATGGTTCAAAAGAAAAGATCAAGGACCAAAATATTAAGCTTGGAGATAAAGCAAAGGAATCTGATTCAAGAAAAAGGTCTTCCCATGTTGATGTCGACGAGGACAAAAGAGAAACAGATAGACTCCGTAGAGATAGTTCTAAAGAAGATGTGAGCAGAAAAAGAGAACGAACAAAGAATAAGGAGGATGATAGATCAAGACGTAAACATTCTACTGACTCAAGCAGGCACAAGAGAAGGCGCTCCTCTTCCATTGACAGCAGGGATAACTCGGTTAATCATGCCAATCATTCAAGTGATGAATCTTCTGAGGATTCTAAAAGGAAGGTACATTCAAGGAGACGTCACTCATCACGTTCACCTGTCAGGTCTAGGAGAAGACAAGTTTCGCGGTCTCCACATAGCAAGCATTCTCAGCGCAGGCATTCTCCCTACTCTTCTCTTGATAGCAACAGGGGGAAAGGGTCAAGATCCAGATCACCTGTGCGGCGGGGAGGCGCCGGTCTGTGGTCTGTTTGGCTATGTGGGTTTGCGCTGATTGGTCTCTCTTTCTATGCCACTCAGAGGCTGCCTTCCATCAACTATCAGATCATGATGAGGCCCAAGCTCGGTCAACGAGTTGGTGACGTTGCAGAGACGCCGAGGATTATCATATTCTCGGCGCCGAGGTCGTTTAGTGGTCCTGTTGGGGATAGGCAGAGTCTAGCTCTTCGTTCATGGCTAGCTTTGTCACCAGAGATTACGGTTGTGCTGTTCAGCAAAGACCCTTCTGTTGTTTCTTTTGCTCAAGGGTTTGGTTCTCGGGTTGTTGTGGATCCCAACATTGACTTCACGTTCCTCGGTACCCCATTTTTTCATTCGATGATGGCAAGAACATGGTCTTTTTCACCAGACATATCTGTTCTGGTTGATCCAGAAACAATTGTTTTACCTGACTTCATTTCCACCCTGAATTATGCTTACAATCTTGATGATGATTGGCTTCTAGTTGCTTCATCACAAAATGTCTCCTACTTCCCATTCTACTTGGATGAGGATGGAAAGCATTGGCGAAGGGAGGATGGCAAATCAATGAGAACACATGAGTTGCAGGAGATAATTGGTCATAGTTGGCAGCAGACTCCCTGCGAACATAAATTGCTTATGGCTTGGAATAGTGGAGATCTTGCCCTATATTCTGGAGTCCTTCCTCCTTTTTTGTATGGTAGGGGGGTGCACAATAGTTGGGTCATTAATGAGGCCTTGTCATCAGAGCTTAGGTTTGTATTTGATGCCAGTTGGACAATCTCAAGCTTATATCTTATTAGTCAGGAGCATCTGAATAACTGGACTGTTGGAGCTTCCGATGCTTTAATTTTTGAAAGAAGAAGCTGGGAATATGCTGGAAACTCTCATGTTGGAGCATTATATGGATCATTGTCTCATTATGAAACTAATAATTCTAGCTTGATAAAACTTTTGAAATGTGACGGCCAATATATCTTTGTCAACAAAACGGAAAACATTTGTTCGTCTGCATACCGACTTGCTGGCTTGGGTTCCTGGTGGAAGAAGAAAAGTTTGGGTTGGATTGAGGGTGTTAAATCACAAGGCGAGTTATTAGATTGCACTCCTATGGTTCCCACAAAGCATTCAGAACCATTAGACTATCCATTTTCCTTAGAGACACTTCTACCATTAAATGCAGACAAAAATAAGACAATTGTGCTTGCTGCTGCTGGATATAGTTACAAGGACATGCTAATGAGCTGGGTGTGTAGAATGCGCCACCTACAGGTCACAAATTTTATCATTTGTGCCCTCGACCAGGAGATCTACGAGTTCTCTGTCGTGATGGGCCTGCCTGTATTCAGGGATCCAGTTGCACCAAGTGAGATAAGCTTCAATGATTGCCACTTCGGAACAAAATGCTTTCAGAAGGTGACCAAAGTGAAATCCAGAATGGTTTTAAAGATACTCAAGATGGGTTACAACGTACTTCTTAGTGACGTGGATGTGTATTGGTTTGGAAATCCATTGCCATTGCTCTGGTCTTTTGGTCCTGCTGTTCTTGCAGCTCAGTCGGATGAATTCAACAAAACAGGACCAATTAACTTACCTAGGCGCTTGAACTCTGGCTTCTACTTTGCCCGTTCAGATAGTTCGACAATTGCCGCTATGAAGAAGGTCGTGGCACATGCAGCAACTTCGGGTCTATCTGAACAGCCAAGCTTCTATGATACACTATGTGGAGAAGGGGGATCCAATCGTGTGGGTGCCAATAGATGCTTAGAACCTGAAACAAATCTGACTGTCCATTTCTTGGACAGAGATCTCTTCCCAAATGGGGCATATCTAGAACTATGGCATAGGAAAAATGTGAGAGCAGCCTGTGTGAAGCAGGGATGTTTGGTTATCCATAACAATTGGATCAGTGGGAGGCTGAAGAAGCTAGAAAGACAGATGTTATCTGGCCTATGGGACTTTGATACTAGCACAAGAATGTGTCAGGCACAACTATGA

Protein Analysis

1603

Amino Acids

179.03

Weight (kDa)

6.47

Isoelectric Point (pI)

62.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleotid_trans PF03407 1363 - 1583 1.8e-45 Nucleotide-diphospho-sugar transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2016
AarI CACCTGC 1 cut(s) 61
AatII GACGTC 1 cut(s) 2656
Acc16I TGCGCA 1 cut(s) 297
Acc36I ACCTGC 1 cut(s) 61
Acc65I GGTACC 2 cut(s) 709, 3152
AccB1I GGYRCC 5 cut(s) 709, 2807, 2971, 3152, 4565
AccB7I CCANNNNNTGG 3 cut(s) 1193, 1874, 4612
AccI GTMKAC 3 cut(s) 237, 2217, 2406
AccII CGCG 3 cut(s) 149, 1915, 2701
AcoI YGGCCR 3 cut(s) 362, 602, 3781
AcsI RAATTY 7 cut(s) 793, 905, 1018, 1348, 1848, 4093, 4379
AcuI CTGAAG 5 cut(s) 1488, 1566, 1644, 1764, 4754
AcyI GRCGYC 4 cut(s) 2653, 2808, 2948, 2972
AdeI CACNNNGTG 4 cut(s) 1046, 1781, 4181, 4538
AfaI GTAC 9 cut(s) 35, 71, 155, 173, 711, 1774, 2640, 3154, 4287
AjiI CACGTC 2 cut(s) 60, 4300
AjuI GAANNNNNNNTTGG 4 cut(s) 3122, 3154, 3854, 3886
Alw21I GWGCWC 5 cut(s) 768, 940, 1575, 1962, 3528
Alw26I GTCTC 9 cut(s) 552, 1733, 2644, 2709, 2858, 2939, 3322, 3735, 3968
Alw44I GTGCAC 1 cut(s) 3524
AlwNI CAGNNNCTG 2 cut(s) 1223, 2878
Ama87I CYCGRG 1 cut(s) 3111
AoxI GGCC 9 cut(s) 41, 362, 602, 2279, 2909, 3549, 3781, 4147, 4762
ApaLI GTGCAC 1 cut(s) 3524
ApoI RAATTY 7 cut(s) 793, 905, 1018, 1348, 1848, 4093, 4379
ArsI GACNNNNNNTTYG 2 cut(s) 1397, 1429
AseI ATTAAT 1 cut(s) 3543
Asp718I GGTACC 2 cut(s) 709, 3152
AspA2I CCTAGG 1 cut(s) 4409
AsuC2I CCSGG 1 cut(s) 258
AsuNHI GCTAGC 1 cut(s) 3031
AvaI CYCGRG 1 cut(s) 3111
AvrII CCTAGG 1 cut(s) 4409
AxyI CCTNAGG 2 cut(s) 339, 799
BaeGI GKGCMC 2 cut(s) 3528, 4111
BamHI GGATCC 4 cut(s) 64, 3124, 4165, 4550
BanI GGYRCC 5 cut(s) 709, 2807, 2971, 3152, 4565
BanII GRGCYC 2 cut(s) 263, 1575
BbsI GAAGAC 3 cut(s) 645, 2382, 2695
Bbv12I GWGCWC 5 cut(s) 768, 940, 1575, 1962, 3528
BbvCI CCTCAGC 1 cut(s) 126
BceAI ACGGC 3 cut(s) 462, 1664, 3796
BcgI CGANNNNNNTGC 6 cut(s) 287, 321, 4353, 4387, 4479, 4513
BciVI GTATCC 2 cut(s) 537, 1547
BclI TGATCA 3 cut(s) 928, 1426, 2170
BcnI CCSGG 1 cut(s) 258
BcoDI GTCTC 9 cut(s) 552, 1733, 2644, 2709, 2858, 2939, 3322, 3735, 3968
BfmI CTRYAG 7 cut(s) 750, 867, 1215, 1299, 1509, 2091, 4082
BfoI RGCGCY 5 cut(s) 1288, 2554, 2811, 2975, 4417
BfuAI ACCTGC 1 cut(s) 61
BfuI GTATCC 2 cut(s) 537, 1547
BglI GCCNNNNNGGC 1 cut(s) 1312
BglII AGATCT 5 cut(s) 677, 1409, 3472, 4122, 4621
BlnI CCTAGG 1 cut(s) 4409
BlpI GCTNAGC 1 cut(s) 1704
BmeT110I CYCGRG 1 cut(s) 3111
BmgBI CACGTC 2 cut(s) 60, 4300
BmtI GCTAGC 1 cut(s) 3035
BpiI GAAGAC 3 cut(s) 645, 2382, 2695
BplI GAGNNNNNCTC 2 cut(s) 3001, 3033
BpmI CTGGAG 5 cut(s) 468, 1157, 1753, 1926, 3510
Bpu10I CCTNAGC 2 cut(s) 126, 3566
Bpu1102I GCTNAGC 1 cut(s) 1704
BpuEI CTTGAG 5 cut(s) 1352, 2515, 3081, 3583, 4253
BpuMI CCSGG 1 cut(s) 258
BsaBI GATNNNNATC 3 cut(s) 2052, 2256, 2785
BsaHI GRCGYC 4 cut(s) 2653, 2808, 2948, 2972
BsaI GGTCTC 2 cut(s) 2709, 2858
BsaWI WCCGGW 1 cut(s) 1120
BsaXI ACNNNNNCTCC 4 cut(s) 105, 135, 1183, 1213
Bse118I RCCGGY 4 cut(s) 488, 592, 600, 2810
Bse21I CCTNAGG 2 cut(s) 339, 799
Bse3DI GCAATG 4 cut(s) 456, 745, 4325, 4331
Bse8I GATNNNNATC 3 cut(s) 2052, 2256, 2785
BseJI GATNNNNATC 3 cut(s) 2052, 2256, 2785
BseMI GCAATG 4 cut(s) 456, 745, 4325, 4331
BseRI GAGGAG 4 cut(s) 195, 273, 312, 2545
BseSI GKGCMC 2 cut(s) 3528, 4111
BseX3I CGGCCG 1 cut(s) 362
BseYI CCCAGC 4 cut(s) 388, 1675, 3681, 4059
Bsh1236I CGCG 3 cut(s) 149, 1915, 2701
Bsh1285I CGRYCG 1 cut(s) 365
BshFI GGCC 9 cut(s) 43, 364, 604, 2281, 2911, 3551, 3783, 4149, 4764
BshNI GGYRCC 5 cut(s) 709, 2807, 2971, 3152, 4565
BsiEI CGRYCG 1 cut(s) 365
BsiHKAI GWGCWC 5 cut(s) 768, 940, 1575, 1962, 3528
BsiHKCI CYCGRG 1 cut(s) 3111
BsiSI CCGG 7 cut(s) 257, 489, 593, 601, 1121, 1985, 2811
BslFI GGGAC 5 cut(s) 178, 601, 1109, 2080, 4784
BsmAI GTCTC 9 cut(s) 552, 1733, 2644, 2709, 2858, 2939, 3322, 3735, 3968
BsmBI CGTCTC 2 cut(s) 2644, 2939
BsmFI GGGAC 5 cut(s) 178, 601, 1109, 2080, 4784
BsmI GAATGC 4 cut(s) 2719, 2734, 3940, 4077
BsnI GGCC 9 cut(s) 43, 364, 604, 2281, 2911, 3551, 3783, 4149, 4764
Bso31I GGTCTC 2 cut(s) 2709, 2858
BsoBI CYCGRG 1 cut(s) 3111
Bsp1286I GDGCHC 7 cut(s) 263, 768, 940, 1575, 1962, 3528, 4111
Bsp1720I GCTNAGC 1 cut(s) 1704
Bsp19I CCATGG 1 cut(s) 1356
BspANI GGCC 9 cut(s) 43, 364, 604, 2281, 2911, 3551, 3783, 4149, 4764
BspFNI CGCG 3 cut(s) 149, 1915, 2701
BspHI TCATGA 4 cut(s) 517, 961, 1906, 2901
BspMAI CTGCAG 5 cut(s) 754, 871, 1219, 1303, 1513
BspMI ACCTGC 1 cut(s) 61
BspOI GCTAGC 1 cut(s) 3035
BspQI GCTCTTC 2 cut(s) 1950, 3024
BspT107I GGYRCC 5 cut(s) 709, 2807, 2971, 3152, 4565
BspTNI GGTCTC 2 cut(s) 2709, 2858
BsrDI GCAATG 4 cut(s) 456, 745, 4325, 4331
BsrFI RCCGGY 4 cut(s) 488, 592, 600, 2810
BssAI RCCGGY 4 cut(s) 488, 592, 600, 2810
BssNI GRCGYC 4 cut(s) 2653, 2808, 2948, 2972
BssT1I CCWWGG 5 cut(s) 373, 420, 1356, 1692, 4409
Bst6I CTCTTC 6 cut(s) 1467, 1950, 2563, 2752, 3024, 4631
BstACI GRCGYC 4 cut(s) 2653, 2808, 2948, 2972
BstAPI GCANNNNNTGC 3 cut(s) 823, 1223, 1805
BstC8I GCNNGC 8 cut(s) 490, 2537, 2717, 2732, 3033, 3841, 4020, 4151
BstDSI CCRYGG 1 cut(s) 1356
BstEII GGTNACC 1 cut(s) 4231
BstENI CCTNNNNNAGG 1 cut(s) 4115
BstFNI CGCG 3 cut(s) 149, 1915, 2701
BstH2I RGCGCY 5 cut(s) 1288, 2554, 2811, 2975, 4417
BstMAI GTCTC 9 cut(s) 552, 1733, 2644, 2709, 2858, 2939, 3322, 3735, 3968
BstMCI CGRYCG 1 cut(s) 365
BstNSI RCATGY 2 cut(s) 4051, 4490
BstPI GGTNACC 1 cut(s) 4231
BstSFI CTRYAG 7 cut(s) 750, 867, 1215, 1299, 1509, 2091, 4082
BstSLI GKGCMC 2 cut(s) 3528, 4111
BstUI CGCG 3 cut(s) 149, 1915, 2701
BstV2I GAAGAC 3 cut(s) 645, 2382, 2695
BstXI CCANNNNNNTGG 2 cut(s) 4338, 4561
BstZI CGGCCG 1 cut(s) 362
Bsu36I CCTNAGG 2 cut(s) 339, 799
BsuI GTATCC 2 cut(s) 537, 1547
BsuRI GGCC 9 cut(s) 43, 364, 604, 2281, 2911, 3551, 3783, 4149, 4764
BtgI CCRYGG 1 cut(s) 1356
BtrI CACGTC 2 cut(s) 60, 4300
BtsI GCAGTG 3 cut(s) 1212, 1308, 1923
BtsIMutI CAGTG 7 cut(s) 221, 1191, 1212, 1308, 1923, 2227, 4729
BveI ACCTGC 1 cut(s) 61
Cac8I GCNNGC 8 cut(s) 490, 2537, 2717, 2732, 3033, 3841, 4020, 4151
CaiI CAGNNNCTG 2 cut(s) 1223, 2878
CciI TCATGA 4 cut(s) 517, 961, 1906, 2901
Cfr10I RCCGGY 4 cut(s) 488, 592, 600, 2810
CseI GACGC 2 cut(s) 752, 2956
CsiI ACCWGGT 2 cut(s) 1042, 1777
Csp6I GTAC 9 cut(s) 34, 70, 154, 172, 710, 1773, 2639, 3153, 4286
CspCI CAANNNNNGTGG 2 cut(s) 131, 166
CviQI GTAC 9 cut(s) 34, 70, 154, 172, 710, 1773, 2639, 3153, 4286
DinI GGCGCC 2 cut(s) 2809, 2973
DraI TTTAAA 1 cut(s) 4260
DraIII CACNNNGTG 4 cut(s) 1046, 1781, 4181, 4538
EaeI YGGCCR 3 cut(s) 362, 602, 3781
EagI CGGCCG 1 cut(s) 362
Eam1104I CTCTTC 6 cut(s) 1467, 1950, 2563, 2752, 3024, 4631
EarI CTCTTC 6 cut(s) 1467, 1950, 2563, 2752, 3024, 4631
EciI GGCGGA 3 cut(s) 90, 213, 261
Ecl136II GAGCTC 1 cut(s) 1573
EclXI CGGCCG 1 cut(s) 362
Eco130I CCWWGG 5 cut(s) 373, 420, 1356, 1692, 4409
Eco147I AGGCCT 2 cut(s) 2281, 3551
Eco24I GRGCYC 2 cut(s) 263, 1575
Eco31I GGTCTC 2 cut(s) 2709, 2858
Eco52I CGGCCG 1 cut(s) 362
Eco53kI GAGCTC 1 cut(s) 1573
Eco57I CTGAAG 5 cut(s) 1488, 1566, 1644, 1764, 4754
Eco81I CCTNAGG 2 cut(s) 339, 799
Eco88I CYCGRG 1 cut(s) 3111
Eco91I GGTNACC 1 cut(s) 4231
EcoICRI GAGCTC 1 cut(s) 1573
EcoNI CCTNNNNNAGG 1 cut(s) 4115
EcoO65I GGTNACC 1 cut(s) 4231
EcoRI GAATTC 2 cut(s) 1018, 4379
EcoT14I CCWWGG 5 cut(s) 373, 420, 1356, 1692, 4409
EcoT22I ATGCAT 1 cut(s) 1261
EcoT38I GRGCYC 2 cut(s) 263, 1575
EgeI GGCGCC 2 cut(s) 2809, 2973
EheI GGCGCC 2 cut(s) 2809, 2973
ErhI CCWWGG 5 cut(s) 373, 420, 1356, 1692, 4409
Esp3I CGTCTC 2 cut(s) 2644, 2939
FalI AAGNNNNNCTT 2 cut(s) 2179, 2211
FaqI GGGAC 5 cut(s) 178, 601, 1109, 2080, 4784
FauI CCCGC 2 cut(s) 261, 2793
FauNDI CATATG 1 cut(s) 822
FbaI TGATCA 3 cut(s) 928, 1426, 2170
FblI GTMKAC 3 cut(s) 237, 2217, 2406
FriOI GRGCYC 2 cut(s) 263, 1575
FspI TGCGCA 1 cut(s) 297
GsaI CCCAGC 4 cut(s) 392, 1679, 3685, 4063
GsuI CTGGAG 5 cut(s) 468, 1157, 1753, 1926, 3510
HaeII RGCGCY 5 cut(s) 1288, 2554, 2811, 2975, 4417
HaeIII GGCC 9 cut(s) 43, 364, 604, 2281, 2911, 3551, 3783, 4149, 4764
HapII CCGG 7 cut(s) 257, 489, 593, 601, 1121, 1985, 2811
HgaI GACGC 2 cut(s) 752, 2956
Hin1I GRCGYC 4 cut(s) 2653, 2808, 2948, 2972
HincII GTYRAC 5 cut(s) 238, 1405, 2407, 2924, 3799
HindII GTYRAC 5 cut(s) 238, 1405, 2407, 2924, 3799
HindIII AAGCTT 5 cut(s) 1706, 2348, 3601, 4189, 4519
HpaII CCGG 7 cut(s) 257, 489, 593, 601, 1121, 1985, 2811
Hpy99I CGWCG 3 cut(s) 64, 202, 2411
HpyCH4IV ACGT 8 cut(s) 59, 2512, 2653, 2665, 2937, 3143, 4284, 4299
HpySE526I ACGT 8 cut(s) 59, 2512, 2653, 2665, 2937, 3143, 4284, 4299
Hsp92I GRCGYC 4 cut(s) 2653, 2808, 2948, 2972
KasI GGCGCC 2 cut(s) 2807, 2971
KpnI GGTACC 2 cut(s) 713, 3156
KroI GCCGGC 1 cut(s) 488
KroNI GCCGGC 1 cut(s) 490
Ksp22I TGATCA 3 cut(s) 928, 1426, 2170
LguI GCTCTTC 2 cut(s) 1950, 3024
LmnI GCTCC 7 cut(s) 647, 1141, 1772, 2558, 3622, 3647, 3710
MabI ACCWGGT 2 cut(s) 1042, 1777
MaeII ACGT 8 cut(s) 59, 2512, 2653, 2665, 2937, 3143, 4284, 4299
MfeI CAATTG 4 cut(s) 3231, 4011, 4458, 4714
MhlI GDGCHC 7 cut(s) 263, 768, 940, 1575, 1962, 3528, 4111
Mly113I GGCGCC 2 cut(s) 2808, 2972
MlyI GAGTC 9 cut(s) 367, 1635, 1919, 2153, 2427, 2522, 3019, 3424, 3501
MmeI TCCRAC 8 cut(s) 42, 1211, 1609, 3569, 3625, 3688, 3857, 4353
Mph1103I ATGCAT 1 cut(s) 1261
MroNI GCCGGC 1 cut(s) 488
MslI CAYNNNNRTG 8 cut(s) 399, 516, 2400, 2604, 3923, 4052, 4790, 4807
MspI CCGG 7 cut(s) 257, 489, 593, 601, 1121, 1985, 2811
MunI CAATTG 4 cut(s) 3231, 4011, 4458, 4714
Mva1269I GAATGC 4 cut(s) 2719, 2734, 3940, 4077
MvnI CGCG 3 cut(s) 149, 1915, 2701
NaeI GCCGGC 1 cut(s) 490
NarI GGCGCC 2 cut(s) 2808, 2972
NciI CCSGG 1 cut(s) 258
NcoI CCATGG 1 cut(s) 1356
NdeI CATATG 1 cut(s) 822
NgoMIV GCCGGC 1 cut(s) 488
NheI GCTAGC 1 cut(s) 3031
NmeAIII GCCGAG 3 cut(s) 2948, 2976, 3000
NmuCI GTSAC 7 cut(s) 2654, 2933, 3039, 3776, 4087, 4231, 4295
NsbI TGCGCA 1 cut(s) 297
NsiI ATGCAT 1 cut(s) 1261
NspI RCATGY 2 cut(s) 4051, 4490
PagI TCATGA 4 cut(s) 517, 961, 1906, 2901
PaqCI CACCTGC 1 cut(s) 61
PceI AGGCCT 2 cut(s) 2281, 3551
PciSI GCTCTTC 2 cut(s) 1950, 3024
PcsI WCGNNNNNNNCGW 1 cut(s) 65
PctI GAATGC 4 cut(s) 2719, 2734, 3940, 4077
PdiI GCCGGC 1 cut(s) 490
PfeI GAWTC 9 cut(s) 5, 965, 1451, 1733, 2257, 2369, 2375, 2612, 2624
PflMI CCANNNNNTGG 3 cut(s) 1193, 1874, 4612
PfoI TCCNGGA 2 cut(s) 1159, 2147
PleI GAGTC 9 cut(s) 366, 1635, 1918, 2152, 2427, 2522, 3018, 3424, 3500
PluTI GGCGCC 2 cut(s) 2811, 2975
PpsI GAGTC 9 cut(s) 366, 1635, 1918, 2152, 2427, 2522, 3018, 3424, 3500
PshBI ATTAAT 1 cut(s) 3543
PsiI TTATAA 1 cut(s) 2016
Psp124BI GAGCTC 1 cut(s) 1575
PspEI GGTNACC 1 cut(s) 4231
PspFI CCCAGC 4 cut(s) 388, 1675, 3681, 4059
PsrI GAACNNNNNNTAC 4 cut(s) 2176, 2208, 2431, 2463
PstI CTGCAG 5 cut(s) 754, 871, 1219, 1303, 1513
PstNI CAGNNNCTG 2 cut(s) 1223, 2878
RsaI GTAC 9 cut(s) 35, 71, 155, 173, 711, 1774, 2640, 3154, 4287
RsaNI GTAC 9 cut(s) 34, 70, 154, 172, 710, 1773, 2639, 3153, 4286
RseI CAYNNNNRTG 8 cut(s) 399, 516, 2400, 2604, 3923, 4052, 4790, 4807
SacI GAGCTC 1 cut(s) 1575
SalI GTCGAC 2 cut(s) 236, 2405
SapI GCTCTTC 2 cut(s) 1950, 3024
SchI GAGTC 9 cut(s) 367, 1635, 1919, 2153, 2427, 2522, 3019, 3424, 3501
SduI GDGCHC 7 cut(s) 263, 768, 940, 1575, 1962, 3528, 4111
SexAI ACCWGGT 2 cut(s) 1042, 1777
SfcI CTRYAG 7 cut(s) 750, 867, 1215, 1299, 1509, 2091, 4082
SfoI GGCGCC 2 cut(s) 2809, 2973
SmiMI CAYNNNNRTG 8 cut(s) 399, 516, 2400, 2604, 3923, 4052, 4790, 4807
SmlI CTYRAG 5 cut(s) 1367, 2530, 3096, 3598, 4268
SmoI CTYRAG 5 cut(s) 1367, 2530, 3096, 3598, 4268
SseBI AGGCCT 2 cut(s) 2281, 3551
SspDI GGCGCC 2 cut(s) 2807, 2971
SspI AATATT 1 cut(s) 2344
SstI GAGCTC 1 cut(s) 1575
StuI AGGCCT 2 cut(s) 2281, 3551
StyI CCWWGG 5 cut(s) 373, 420, 1356, 1692, 4409
TaiI ACGT 8 cut(s) 62, 2515, 2656, 2668, 2940, 3146, 4287, 4302
TaqI TCGA 6 cut(s) 158, 237, 2406, 3170, 4113, 4454
TaqII GACCGA 1 cut(s) 2909
TatI WGTACW 2 cut(s) 33, 1772
TauI GCSGC 3 cut(s) 229, 2800, 4466
TfiI GAWTC 9 cut(s) 5, 965, 1451, 1733, 2257, 2369, 2375, 2612, 2624
TscAI CASTG 7 cut(s) 221, 1198, 1219, 1308, 1930, 2227, 4729
TseFI GTSAC 7 cut(s) 2654, 2933, 3039, 3776, 4087, 4231, 4295
Tsp45I GTSAC 7 cut(s) 2654, 2933, 3039, 3776, 4087, 4231, 4295
TspGWI ACGGA 5 cut(s) 57, 330, 1278, 2426, 3821
TspRI CASTG 7 cut(s) 221, 1198, 1219, 1308, 1930, 2227, 4729
Van91I CCANNNNNTGG 3 cut(s) 1193, 1874, 4612
VneI GTGCAC 1 cut(s) 3524
VspI ATTAAT 1 cut(s) 3543
XagI CCTNNNNNAGG 1 cut(s) 4115
XapI RAATTY 7 cut(s) 793, 905, 1018, 1348, 1848, 4093, 4379
XbaI TCTAGA 2 cut(s) 1457, 4645
XceI RCATGY 2 cut(s) 4051, 4490
XmaJI CCTAGG 1 cut(s) 4409
XmiI GTMKAC 3 cut(s) 237, 2217, 2406
ZraI GACGTC 1 cut(s) 2654
Zsp2I ATGCAT 1 cut(s) 1261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.