MD16G1118000.v1.1

arabinosyltransferase ARAD1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008092
Physical Location & Seq
Forward (+)
8392348 .. 8395609
3262 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1118000.v1.1.491

Sequence Viewer

Length: 1329 bp
ATGCCAAACGCCAGATCTCACAAAATGTATGGAAAAGCCGCCTTCGCCCTCATCTTCGCCTTCCTCCTGCTCCTCACCTACTCAATCTTCATCGGCACCGTCGACATCCGATCCTACTTCCTCCCGCTCCTCCCCTCATCGCCGCCGCCGGTTGTCCAGTCCCTCTGCGCCACCACCTCCCCGCCTCTCAAGGTCTACATGTACGATCTCCCGCGCCGCTTCAACGTCGGAATTATGAACCGCCGGAGCACCGACCAGACTCCTGTCACCGCCCGGACTTGGCCTCCGTGGCCAAAGAACTCCGGGCTCAAGAGGCAGCACAGTATTGAGTACTGGATGATGGGCTCGCTTTTGTTCGACAGTAATGGCGGTGACGGGAGAGAGGCGGTTAGGGTTTCTGATCCGGAATTGGCCGATGCGTTCTTTGTGCCGTTTTTCTCTTCGTTGAGCTTCAATACTCATGGGCACAACATGACAGACCCGGATACTCGGATTGATCGCCAATTGCAGATAGATTTATTGAAAATCTTGGGAGAATCAAAGTACTGGCAAAGATCTGGAGGGCGAGACCATGTTATCCCTTTGACACATCCCAATGCTTTCAGATTTCTTCGGCCACAGGTCAATGCGTCAATTCAGATCGTTGTAGATTTTGGCAGGTATCCTCATGTAATGTCAAATCTGAGCAAAGATGTGGTCACCCCATACGTACATGTTGTGGATTCCTTTACAGATGATGACCGTCCAGATCCATTTGAGTCCCGTACTACGCTTCTTTTCTTCCAGGGAAGGACATACAGGAAAGATGAAGGCATTGTTCGTGTTAAACTGGCAAAAGTCTTAGCTGGTTATGATGATGTTCACTATGAGCGAAGTGTTGCAACAGGAGAGAACATAAAATTGTCTACGCGACGAATGCGTTTATCAAAGTTCTGTCTGCATCCTGCTGGAGACACTCCTTCATCTTGTCGCCTATTTGATGCTATTGTGAGCCACTGTGTTCCTGTCATCGTGAGTGATGAAATTGAGCTCCCGTTTGAGGATGAAATTGACTACACCAAATTCTCACTATTCTTCTCATTCAAAGAGGCTTTGAAACCTGATTACATGGTTAATCAGCTCCGTAATGTTTCAAAGGAGAGATGGATTGAAATGTGGAGGACACTTAAGAATATCTCCCACCATTTTGAATTCAATTACCCCCCAGAGAAAGAGGATGCAGTCGATATGCTATGGAGACAGGTGAAGCACAAGCTCTCTGCTGTCAAACTTTCTGTACATAGAATCCAAAGGTTGAAAATACCAGACTGGTGGCGGAGGAGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

443

Amino Acids

51.35

Weight (kDa)

9.35

Isoelectric Point (pI)

50.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 61 - 373 3.5e-72 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 648
AccB1I GGYRCC 1 cut(s) 95
AccBSI CCGCTC 1 cut(s) 127
AccI GTMKAC 3 cut(s) 102, 195, 905
AccII CGCG 2 cut(s) 214, 910
AccIII TCCGGA 1 cut(s) 403
AclWI GGATC 3 cut(s) 105, 395, 743
AcoI YGGCCR 3 cut(s) 290, 411, 614
AcsI RAATTY 2 cut(s) 1061, 1190
AfaI GTAC 6 cut(s) 203, 332, 545, 711, 766, 1278
AfiI CCNNNNNNNGG 2 cut(s) 279, 1039
AflII CTTAAG 1 cut(s) 1166
AflIII ACRYGT 2 cut(s) 198, 712
AjnI CCWGG 1 cut(s) 783
AluBI AGCT 5 cut(s) 450, 845, 1030, 1120, 1255
AluI AGCT 5 cut(s) 450, 845, 1030, 1120, 1255
Alw21I GWGCWC 2 cut(s) 251, 1032
Alw26I GTCTC 3 cut(s) 561, 945, 1231
AlwI GGATC 3 cut(s) 105, 395, 743
Aor13HI TCCGGA 1 cut(s) 403
AoxI GGCC 4 cut(s) 281, 290, 411, 614
ApeKI GCWGC 1 cut(s) 316
ApoI RAATTY 2 cut(s) 1061, 1190
ArsI GACNNNNNNTTYG 2 cut(s) 681, 713
AspLEI GCGC 2 cut(s) 170, 216
AsuC2I CCSGG 3 cut(s) 274, 304, 482
AsuHPI GGTGA 5 cut(s) 67, 259, 383, 691, 1255
BaeGI GKGCMC 1 cut(s) 468
BalI TGGCCA 1 cut(s) 292
BanI GGYRCC 1 cut(s) 95
BanII GRGCYC 3 cut(s) 309, 347, 1032
Bbv12I GWGCWC 2 cut(s) 251, 1032
BbvI GCAGC 1 cut(s) 328
BccI CCATC 2 cut(s) 334, 1137
BceAI ACGGC 1 cut(s) 415
BciT130I CCWGG 1 cut(s) 785
BciVI GTATCC 2 cut(s) 478, 672
BcnI CCSGG 3 cut(s) 274, 304, 482
BcoDI GTCTC 3 cut(s) 561, 945, 1231
BfrI CTTAAG 1 cut(s) 1166
BfuAI ACCTGC 1 cut(s) 648
BfuI GTATCC 2 cut(s) 478, 672
BglI GCCNNNNNGGC 1 cut(s) 289
BglII AGATCT 2 cut(s) 14, 554
BisI GCNGC 5 cut(s) 39, 143, 146, 217, 317
BlsI GCNGC 5 cut(s) 40, 144, 147, 218, 318
BmcAI AGTACT 2 cut(s) 332, 545
Bme1390I CCNGG 4 cut(s) 274, 304, 482, 785
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 4 cut(s) 274, 304, 482, 785
BmsI GCATC 4 cut(s) 406, 949, 970, 1207
BoxI GACNNNNGTC 1 cut(s) 263
BpmI CTGGAG 2 cut(s) 579, 969
BpuEI CTTGAG 2 cut(s) 173, 293
BpuMI CCSGG 3 cut(s) 274, 304, 482
BsaAI YACGTR 1 cut(s) 709
BsaI GGTCTC 1 cut(s) 561
BsaJI CCNNGG 2 cut(s) 287, 784
BsaWI WCCGGW 1 cut(s) 403
BsaXI ACNNNNNCTCC 2 cut(s) 268, 298
Bsc4I CCNNNNNNNGG 2 cut(s) 279, 1039
Bse118I RCCGGY 1 cut(s) 148
Bse1I ACTGG 5 cut(s) 157, 338, 551, 834, 1313
BseAI TCCGGA 1 cut(s) 403
BseBI CCWGG 1 cut(s) 785
BseDI CCNNGG 2 cut(s) 287, 784
BseGI GGATG 6 cut(s) 105, 342, 589, 940, 1048, 1222
BseLI CCNNNNNNNGG 2 cut(s) 279, 1039
BseMII CTCAG 1 cut(s) 674
BseNI ACTGG 5 cut(s) 157, 338, 551, 834, 1313
BseRI GAGGAG 2 cut(s) 62, 119
BseSI GKGCMC 1 cut(s) 468
BseXI GCAGC 1 cut(s) 328
Bsh1236I CGCG 2 cut(s) 214, 910
BshFI GGCC 4 cut(s) 283, 292, 413, 616
BshNI GGYRCC 1 cut(s) 95
BsiHKAI GWGCWC 2 cut(s) 251, 1032
BsiSI CCGG 6 cut(s) 149, 244, 274, 303, 404, 482
BslFI GGGAC 2 cut(s) 145, 745
BslI CCNNNNNNNGG 2 cut(s) 279, 1039
BsmAI GTCTC 3 cut(s) 561, 945, 1231
BsmFI GGGAC 2 cut(s) 145, 745
BsmI GAATGC 1 cut(s) 921
BsnI GGCC 4 cut(s) 283, 292, 413, 616
Bso31I GGTCTC 1 cut(s) 561
Bsp1286I GDGCHC 5 cut(s) 251, 309, 347, 468, 1032
Bsp13I TCCGGA 1 cut(s) 403
Bsp1407I TGTACA 1 cut(s) 1276
Bsp143I GATC 8 cut(s) 14, 110, 205, 400, 496, 554, 639, 748
BspANI GGCC 4 cut(s) 283, 292, 413, 616
BspCNI CTCAG 1 cut(s) 675
BspEI TCCGGA 1 cut(s) 403
BspFNI CGCG 2 cut(s) 214, 910
BspLI GGNNCC 1 cut(s) 97
BspMI ACCTGC 1 cut(s) 648
BspPI GGATC 3 cut(s) 105, 395, 743
BspT107I GGYRCC 1 cut(s) 95
BspTI CTTAAG 1 cut(s) 1166
BspTNI GGTCTC 1 cut(s) 561
BsrBI CCGCTC 1 cut(s) 127
BsrFI RCCGGY 1 cut(s) 148
BsrGI TGTACA 1 cut(s) 1276
BsrI ACTGG 5 cut(s) 157, 338, 551, 834, 1313
BssAI RCCGGY 1 cut(s) 148
BssECI CCNNGG 2 cut(s) 287, 784
BssMI GATC 8 cut(s) 14, 110, 205, 400, 496, 554, 639, 748
Bst2UI CCWGG 1 cut(s) 785
Bst4CI ACNGT 5 cut(s) 100, 323, 362, 743, 998
Bst6I CTCTTC 1 cut(s) 445
BstAFI CTTAAG 1 cut(s) 1166
BstAUI TGTACA 1 cut(s) 1276
BstBAI YACGTR 1 cut(s) 709
BstC8I GCNNGC 1 cut(s) 347
BstDEI CTNAG 2 cut(s) 683, 841
BstDSI CCRYGG 1 cut(s) 287
BstEII GGTNACC 1 cut(s) 697
BstF5I GGATG 6 cut(s) 105, 342, 589, 940, 1048, 1222
BstFNI CGCG 2 cut(s) 214, 910
BstHHI GCGC 2 cut(s) 170, 216
BstKTI GATC 8 cut(s) 17, 113, 208, 403, 499, 557, 642, 751
BstMAI GTCTC 3 cut(s) 561, 945, 1231
BstMBI GATC 8 cut(s) 14, 110, 205, 400, 496, 554, 639, 748
BstMWI GCNNNNNNNGC 4 cut(s) 44, 289, 313, 916
BstNI CCWGG 1 cut(s) 785
BstNSI RCATGY 2 cut(s) 202, 716
BstPAI GACNNNNGTC 1 cut(s) 263
BstPI GGTNACC 1 cut(s) 697
BstSCI CCNGG 4 cut(s) 272, 302, 480, 783
BstSLI GKGCMC 1 cut(s) 468
BstSNI TACGTA 1 cut(s) 709
BstUI CGCG 2 cut(s) 214, 910
BstV1I GCAGC 1 cut(s) 328
BstX2I RGATCY 3 cut(s) 14, 554, 748
BstXI CCANNNNNNTGG 1 cut(s) 1311
BstYI RGATCY 3 cut(s) 14, 554, 748
BsuI GTATCC 2 cut(s) 478, 672
BsuRI GGCC 4 cut(s) 283, 292, 413, 616
BtgI CCRYGG 1 cut(s) 287
BtgZI GCGATG 1 cut(s) 123
BtsCI GGATG 6 cut(s) 105, 342, 589, 940, 1048, 1222
BtsIMutI CAGTG 1 cut(s) 994
BveI ACCTGC 1 cut(s) 648
Cac8I GCNNGC 1 cut(s) 347
CfoI GCGC 2 cut(s) 170, 216
Cfr10I RCCGGY 1 cut(s) 148
CseI GACGC 1 cut(s) 618
Csp6I GTAC 6 cut(s) 202, 331, 544, 710, 765, 1277
CviAII CATG 7 cut(s) 199, 461, 472, 572, 668, 713, 1108
CviQI GTAC 6 cut(s) 202, 331, 544, 710, 765, 1277
DdeI CTNAG 2 cut(s) 683, 841
DpnI GATC 8 cut(s) 16, 112, 207, 402, 498, 556, 641, 750
DpnII GATC 8 cut(s) 14, 110, 205, 400, 496, 554, 639, 748
EaeI YGGCCR 3 cut(s) 290, 411, 614
Eam1104I CTCTTC 1 cut(s) 445
EarI CTCTTC 1 cut(s) 445
Ecl136II GAGCTC 1 cut(s) 1030
Eco105I TACGTA 1 cut(s) 709
Eco24I GRGCYC 3 cut(s) 309, 347, 1032
Eco31I GGTCTC 1 cut(s) 561
Eco53kI GAGCTC 1 cut(s) 1030
Eco91I GGTNACC 1 cut(s) 697
EcoICRI GAGCTC 1 cut(s) 1030
EcoO65I GGTNACC 1 cut(s) 697
EcoRI GAATTC 1 cut(s) 1190
EcoRII CCWGG 1 cut(s) 783
EcoT38I GRGCYC 3 cut(s) 309, 347, 1032
FaeI CATG 7 cut(s) 202, 464, 475, 575, 671, 716, 1111
FaqI GGGAC 2 cut(s) 145, 745
FatI CATG 7 cut(s) 198, 460, 471, 571, 667, 712, 1107
FauI CCCGC 3 cut(s) 132, 189, 219
FblI GTMKAC 3 cut(s) 102, 195, 905
Fnu4HI GCNGC 5 cut(s) 39, 143, 146, 217, 317
FokI GGATG 6 cut(s) 92, 349, 576, 927, 1055, 1229
FriOI GRGCYC 3 cut(s) 309, 347, 1032
Fsp4HI GCNGC 5 cut(s) 39, 143, 146, 217, 317
GlaI GCGC 2 cut(s) 169, 215
GluI GCNGC 5 cut(s) 39, 143, 146, 217, 317
GsuI CTGGAG 2 cut(s) 579, 969
HaeIII GGCC 4 cut(s) 283, 292, 413, 616
HapII CCGG 6 cut(s) 149, 244, 274, 303, 404, 482
HgaI GACGC 1 cut(s) 618
HhaI GCGC 2 cut(s) 170, 216
Hin1II CATG 7 cut(s) 202, 464, 475, 575, 671, 716, 1111
Hin6I GCGC 2 cut(s) 168, 214
HinP1I GCGC 2 cut(s) 168, 214
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HinfI GANTC 5 cut(s) 259, 536, 722, 758, 1284
HpaII CCGG 6 cut(s) 149, 244, 274, 303, 404, 482
HphI GGTGA 5 cut(s) 67, 259, 383, 691, 1255
Hpy166II GTNNAC 4 cut(s) 103, 196, 862, 906
Hpy188I TCNGA 7 cut(s) 110, 230, 400, 492, 605, 639, 684
Hpy188III TCNNGA 5 cut(s) 310, 404, 558, 746, 1012
Hpy8I GTNNAC 4 cut(s) 103, 196, 862, 906
Hpy99I CGWCG 3 cut(s) 104, 230, 915
HpyAV CCTTC 5 cut(s) 52, 70, 783, 803, 969
HpyCH4III ACNGT 5 cut(s) 100, 323, 362, 743, 998
HpyCH4IV ACGT 2 cut(s) 225, 708
HpyCH4V TGCA 4 cut(s) 508, 881, 940, 1220
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 289, 313, 916
HpyF3I CTNAG 2 cut(s) 683, 841
HpySE526I ACGT 2 cut(s) 225, 708
Hsp92II CATG 7 cut(s) 202, 464, 475, 575, 671, 716, 1111
HspAI GCGC 2 cut(s) 168, 214
Kpn2I TCCGGA 1 cut(s) 403
Kzo9I GATC 8 cut(s) 14, 110, 205, 400, 496, 554, 639, 748
LmnI GCTCC 5 cut(s) 75, 132, 246, 1035, 1125
Lsp1109I GCAGC 1 cut(s) 328
LweI GCATC 4 cut(s) 406, 949, 970, 1207
MaeII ACGT 2 cut(s) 225, 708
MaeIII GTNAC 3 cut(s) 265, 371, 697
MalI GATC 8 cut(s) 16, 112, 207, 402, 498, 556, 641, 750
MbiI CCGCTC 1 cut(s) 127
MboI GATC 8 cut(s) 14, 110, 205, 400, 496, 554, 639, 748
MboII GAAGA 6 cut(s) 46, 79, 432, 602, 772, 1066
MfeI CAATTG 1 cut(s) 503
MflI RGATCY 3 cut(s) 14, 554, 748
MhlI GDGCHC 5 cut(s) 251, 309, 347, 468, 1032
MlsI TGGCCA 1 cut(s) 292
MluNI TGGCCA 1 cut(s) 292
MlyI GAGTC 2 cut(s) 253, 767
MmeI TCCRAC 1 cut(s) 208
Mox20I TGGCCA 1 cut(s) 292
MroI TCCGGA 1 cut(s) 403
MscI TGGCCA 1 cut(s) 292
MseI TTAA 3 cut(s) 825, 1113, 1167
MslI CAYNNNNRTG 1 cut(s) 594
Msp20I TGGCCA 1 cut(s) 292
MspCI CTTAAG 1 cut(s) 1166
MspI CCGG 6 cut(s) 149, 244, 274, 303, 404, 482
MspR9I CCNGG 4 cut(s) 274, 304, 482, 785
MunI CAATTG 1 cut(s) 503
Mva1269I GAATGC 1 cut(s) 921
MvaI CCWGG 1 cut(s) 785
MvnI CGCG 2 cut(s) 214, 910
MwoI GCNNNNNNNGC 4 cut(s) 44, 289, 313, 916
NciI CCSGG 3 cut(s) 274, 304, 482
NdeII GATC 8 cut(s) 14, 110, 205, 400, 496, 554, 639, 748
NlaIII CATG 7 cut(s) 202, 464, 475, 575, 671, 716, 1111
NlaIV GGNNCC 1 cut(s) 97
NmuCI GTSAC 3 cut(s) 265, 371, 697
NspI RCATGY 2 cut(s) 202, 716
PciI ACATGT 2 cut(s) 198, 712
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PctI GAATGC 1 cut(s) 921
PfeI GAWTC 3 cut(s) 536, 722, 1284
PkrI GCNGC 5 cut(s) 40, 144, 147, 218, 318
PleI GAGTC 2 cut(s) 253, 766
PpsI GAGTC 2 cut(s) 253, 766
Ppu21I YACGTR 1 cut(s) 709
PscI ACATGT 2 cut(s) 198, 712
PshAI GACNNNNGTC 1 cut(s) 263
Psp124BI GAGCTC 1 cut(s) 1032
Psp6I CCWGG 1 cut(s) 783
PspEI GGTNACC 1 cut(s) 697
PspGI CCWGG 1 cut(s) 783
PspN4I GGNNCC 1 cut(s) 97
PsuI RGATCY 3 cut(s) 14, 554, 748
RsaI GTAC 6 cut(s) 203, 332, 545, 711, 766, 1278
RsaNI GTAC 6 cut(s) 202, 331, 544, 710, 765, 1277
RseI CAYNNNNRTG 1 cut(s) 594
SacI GAGCTC 1 cut(s) 1032
SalI GTCGAC 1 cut(s) 101
SaqAI TTAA 3 cut(s) 825, 1113, 1167
SatI GCNGC 5 cut(s) 39, 143, 146, 217, 317
Sau3AI GATC 8 cut(s) 14, 110, 205, 400, 496, 554, 639, 748
ScaI AGTACT 2 cut(s) 332, 545
SchI GAGTC 2 cut(s) 253, 767
ScrFI CCNGG 4 cut(s) 274, 304, 482, 785
SduI GDGCHC 5 cut(s) 251, 309, 347, 468, 1032
SfaNI GCATC 4 cut(s) 406, 949, 970, 1207
SfiI GGCCNNNNNGGCC 1 cut(s) 289
SmiMI CAYNNNNRTG 1 cut(s) 594
SmlI CTYRAG 3 cut(s) 188, 308, 1166
SmoI CTYRAG 3 cut(s) 188, 308, 1166
SnaBI TACGTA 1 cut(s) 709
SstI GAGCTC 1 cut(s) 1032
StyD4I CCNGG 4 cut(s) 272, 302, 480, 783
TaaI ACNGT 5 cut(s) 100, 323, 362, 743, 998
TaiI ACGT 2 cut(s) 228, 711
TaqI TCGA 3 cut(s) 102, 357, 1224
TatI WGTACW 3 cut(s) 330, 543, 1276
TauI GCSGC 4 cut(s) 41, 145, 148, 219
TfiI GAWTC 3 cut(s) 536, 722, 1284
Tru1I TTAA 3 cut(s) 825, 1113, 1167
Tru9I TTAA 3 cut(s) 825, 1113, 1167
TscAI CASTG 1 cut(s) 1001
TseFI GTSAC 3 cut(s) 265, 371, 697
TseI GCWGC 1 cut(s) 316
Tsp45I GTSAC 3 cut(s) 265, 371, 697
TspDTI ATGAA 6 cut(s) 79, 251, 822, 951, 1035, 1059
TspGWI ACGGA 2 cut(s) 276, 1112
TspRI CASTG 1 cut(s) 1001
Vha464I CTTAAG 1 cut(s) 1166
XapI RAATTY 2 cut(s) 1061, 1190
XceI RCATGY 2 cut(s) 202, 716
XmiI GTMKAC 3 cut(s) 102, 195, 905
ZrmI AGTACT 2 cut(s) 332, 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.