MD16G1143400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016588
Physical Location & Seq
Forward (+)
11018839 .. 11020315
1477 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1143400.v1.1.491

Sequence Viewer

Length: 492 bp
ATGGCTTTCCGGGCAATCAACGAGTTGAGATTAATGCTGGCTCCGGCGGGAGGGAACCGAGCTTTTGCAACCTCCGCCGCTCCGAAGATGAAACATTTTGCAGAAAATGTTCGTGATAGTGAGGCTGCTCAGTCTACCATGTCGAGCATCTGGAAATTTCTTAGAAAGCCAGAGAACGCACCCGTTGCTGTGCTGCTAGGGTTCCTAATTGTGCCTCTGACGATTGGGACGCATAGTGCTCTACAGCAGCTTATGCATGCCCCGATGGTTGTGGTCAACAAGAACAAGAGGGCCATGTACGAGGTTGACAACCCAGATGCTGCCGTTAGCTCATCCGACAAGTTTATCAACAAATCTTTCCTGAGGAAGGTTGGCCACATTCAGGAGCCCGGCAGCCCATCCATGCCTGATTCAGTCCAGCATGACCCTTTTACTCGGCCTCGCACGGCGGCGACGCTCAAGACTGTCGGAGTGGATCCAAGCCGTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

164

Amino Acids

17.85

Weight (kDa)

10.21

Isoelectric Point (pI)

63.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016588)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G63060
fragaria_vesca FvH4_4g22290
malus_domestica MD16G1143400.v1.1
prunus_persica Prupe.1G212400_v2.0.a1
pyrus_communis pycom16g11330
rosa_chinensis RchiOBHm_Chr4g0428531
rosa_multiflora Rmu_co8298239.1_g000001
rosa_roxburghii Rroxscaffold_5G00370230
rosa_rugosa Rorug04G0226000
rosa_samantha Rh4AG283500 Rh4BG289200 Rh4CG305100 Rh4DG286200
rosa_wichuraiana Rw4G024610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 80
AccI GTMKAC 1 cut(s) 134
AciI CCGC 4 cut(s) 47, 75, 78, 449
AclWI GGATC 2 cut(s) 470, 483
AcoI YGGCCR 1 cut(s) 373
AcsI RAATTY 1 cut(s) 155
AfaI GTAC 1 cut(s) 299
AfiI CCNNNNNNNGG 3 cut(s) 50, 367, 382
AluBI AGCT 3 cut(s) 62, 250, 330
AluI AGCT 3 cut(s) 62, 250, 330
Alw21I GWGCWC 1 cut(s) 241
AlwI GGATC 2 cut(s) 470, 483
AlwNI CAGNNNCTG 1 cut(s) 320
AoxI GGCC 3 cut(s) 291, 373, 437
ApeKI GCWGC 5 cut(s) 125, 193, 247, 320, 393
ApoI RAATTY 1 cut(s) 155
AseI ATTAAT 1 cut(s) 32
Asp700I GAANNNNTTC 1 cut(s) 108
AspS9I GGNCC 1 cut(s) 291
AsuC2I CCSGG 2 cut(s) 11, 390
AxyI CCTNAGG 1 cut(s) 362
BalI TGGCCA 1 cut(s) 375
BamHI GGATCC 1 cut(s) 475
BanII GRGCYC 1 cut(s) 390
Bbv12I GWGCWC 1 cut(s) 241
BbvI GCAGC 5 cut(s) 112, 180, 259, 307, 405
BccI CCATC 2 cut(s) 259, 406
BceAI ACGGC 3 cut(s) 308, 462, 468
BcnI CCSGG 2 cut(s) 11, 390
BfaI CTAG 1 cut(s) 197
BfmI CTRYAG 1 cut(s) 242
BisI GCNGC 7 cut(s) 78, 126, 194, 248, 321, 394, 450
BlsI GCNGC 7 cut(s) 79, 127, 195, 249, 322, 395, 451
Bme1390I CCNGG 2 cut(s) 11, 390
BmgT120I GGNCC 1 cut(s) 291
BmiI GGNNCC 5 cut(s) 42, 56, 203, 387, 477
BmrFI CCNGG 2 cut(s) 11, 390
BmsI GCATC 2 cut(s) 156, 307
BpuEI CTTGAG 1 cut(s) 443
BpuMI CCSGG 2 cut(s) 11, 390
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 367, 382
Bse21I CCTNAGG 1 cut(s) 362
BseGI GGATG 2 cut(s) 332, 398
BseLI CCNNNNNNNGG 3 cut(s) 50, 367, 382
BseMII CTCAG 2 cut(s) 143, 353
BseXI GCAGC 5 cut(s) 112, 180, 259, 307, 405
BshFI GGCC 3 cut(s) 293, 375, 439
BsiHKAI GWGCWC 1 cut(s) 241
BsiSI CCGG 3 cut(s) 10, 44, 390
BslFI GGGAC 1 cut(s) 241
BslI CCNNNNNNNGG 3 cut(s) 50, 367, 382
BsmFI GGGAC 1 cut(s) 241
BsnI GGCC 3 cut(s) 293, 375, 439
Bsp1286I GDGCHC 2 cut(s) 241, 390
Bsp143I GATC 1 cut(s) 475
BspACI CCGC 4 cut(s) 47, 75, 78, 449
BspANI GGCC 3 cut(s) 293, 375, 439
BspCNI CTCAG 2 cut(s) 142, 354
BspLI GGNNCC 5 cut(s) 42, 56, 203, 387, 477
BspPI GGATC 2 cut(s) 470, 483
BsrBI CCGCTC 1 cut(s) 80
BssMI GATC 1 cut(s) 475
Bst4CI ACNGT 1 cut(s) 466
BstAPI GCANNNNNTGC 2 cut(s) 185, 253
BstC8I GCNNGC 2 cut(s) 39, 258
BstDEI CTNAG 3 cut(s) 129, 161, 362
BstENI CCTNNNNNAGG 1 cut(s) 365
BstF5I GGATG 2 cut(s) 332, 398
BstKTI GATC 1 cut(s) 478
BstMBI GATC 1 cut(s) 475
BstMWI GCNNNNNNNGC 4 cut(s) 11, 74, 185, 253
BstNSI RCATGY 1 cut(s) 260
BstSCI CCNGG 2 cut(s) 9, 388
BstSFI CTRYAG 1 cut(s) 242
BstV1I GCAGC 5 cut(s) 112, 180, 259, 307, 405
BstX2I RGATCY 1 cut(s) 475
BstYI RGATCY 1 cut(s) 475
Bsu36I CCTNAGG 1 cut(s) 362
BsuRI GGCC 3 cut(s) 293, 375, 439
BtsCI GGATG 2 cut(s) 332, 398
Cac8I GCNNGC 2 cut(s) 39, 258
CaiI CAGNNNCTG 1 cut(s) 320
Cfr13I GGNCC 1 cut(s) 291
CseI GACGC 2 cut(s) 238, 463
Csp6I GTAC 1 cut(s) 298
CviAII CATG 5 cut(s) 139, 257, 295, 403, 422
CviQI GTAC 1 cut(s) 298
DdeI CTNAG 3 cut(s) 129, 161, 362
DpnI GATC 1 cut(s) 477
DpnII GATC 1 cut(s) 475
EaeI YGGCCR 1 cut(s) 373
EciI GGCGGA 1 cut(s) 64
Eco24I GRGCYC 1 cut(s) 390
Eco81I CCTNAGG 1 cut(s) 362
EcoNI CCTNNNNNAGG 1 cut(s) 365
EcoT22I ATGCAT 1 cut(s) 258
EcoT38I GRGCYC 1 cut(s) 390
FaeI CATG 5 cut(s) 142, 260, 298, 406, 425
FaiI YATR 7 cut(s) 140, 234, 254, 258, 296, 404, 423
FaqI GGGAC 1 cut(s) 241
FatI CATG 5 cut(s) 138, 256, 294, 402, 421
FauI CCCGC 1 cut(s) 40
FblI GTMKAC 1 cut(s) 134
Fnu4HI GCNGC 7 cut(s) 78, 126, 194, 248, 321, 394, 450
FokI GGATG 2 cut(s) 319, 385
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 7 cut(s) 78, 126, 194, 248, 321, 394, 450
FspBI CTAG 1 cut(s) 197
GluI GCNGC 7 cut(s) 78, 126, 194, 248, 321, 394, 450
HaeIII GGCC 3 cut(s) 293, 375, 439
HapII CCGG 3 cut(s) 10, 44, 390
HgaI GACGC 2 cut(s) 238, 463
Hin1II CATG 5 cut(s) 142, 260, 298, 406, 425
HincII GTYRAC 2 cut(s) 277, 307
HindII GTYRAC 2 cut(s) 277, 307
HinfI GANTC 1 cut(s) 410
HpaII CCGG 3 cut(s) 10, 44, 390
Hpy166II GTNNAC 3 cut(s) 135, 277, 307
Hpy188I TCNGA 4 cut(s) 84, 219, 337, 470
Hpy188III TCNNGA 5 cut(s) 113, 151, 361, 383, 460
Hpy8I GTNNAC 3 cut(s) 135, 277, 307
Hpy99I CGWCG 2 cut(s) 457, 489
HpyAV CCTTC 1 cut(s) 361
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4V TGCA 3 cut(s) 68, 101, 256
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 74, 185, 253
HpyF3I CTNAG 3 cut(s) 129, 161, 362
Hsp92II CATG 5 cut(s) 142, 260, 298, 406, 425
Kzo9I GATC 1 cut(s) 475
LmnI GCTCC 3 cut(s) 46, 85, 385
Lsp1109I GCAGC 5 cut(s) 112, 180, 259, 307, 405
LweI GCATC 2 cut(s) 156, 307
MaeI CTAG 1 cut(s) 197
MalI GATC 1 cut(s) 477
MbiI CCGCTC 1 cut(s) 80
MboI GATC 1 cut(s) 475
MboII GAAGA 1 cut(s) 97
MflI RGATCY 1 cut(s) 475
MhlI GDGCHC 2 cut(s) 241, 390
MlsI TGGCCA 1 cut(s) 375
MluCI AATT 2 cut(s) 155, 207
MluNI TGGCCA 1 cut(s) 375
MmeI TCCRAC 2 cut(s) 360, 448
MnlI CCTC 8 cut(s) 44, 82, 115, 225, 282, 295, 357, 450
Mox20I TGGCCA 1 cut(s) 375
Mph1103I ATGCAT 1 cut(s) 258
MroXI GAANNNNTTC 1 cut(s) 108
MscI TGGCCA 1 cut(s) 375
MseI TTAA 1 cut(s) 32
Msp20I TGGCCA 1 cut(s) 375
MspI CCGG 3 cut(s) 10, 44, 390
MspR9I CCNGG 2 cut(s) 11, 390
MwoI GCNNNNNNNGC 4 cut(s) 11, 74, 185, 253
NciI CCSGG 2 cut(s) 11, 390
NdeII GATC 1 cut(s) 475
NlaIII CATG 5 cut(s) 142, 260, 298, 406, 425
NlaIV GGNNCC 5 cut(s) 42, 56, 203, 387, 477
NmeAIII GCCGAG 1 cut(s) 415
NsiI ATGCAT 1 cut(s) 258
NspI RCATGY 1 cut(s) 260
PaeI GCATGC 1 cut(s) 260
PdmI GAANNNNTTC 1 cut(s) 108
PfeI GAWTC 1 cut(s) 410
PkrI GCNGC 7 cut(s) 79, 127, 195, 249, 322, 395, 451
PshBI ATTAAT 1 cut(s) 32
PspN4I GGNNCC 5 cut(s) 42, 56, 203, 387, 477
PspPI GGNCC 1 cut(s) 291
PstNI CAGNNNCTG 1 cut(s) 320
PsuI RGATCY 1 cut(s) 475
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 7 cut(s) 78, 126, 194, 248, 321, 394, 450
Sau3AI GATC 1 cut(s) 475
Sau96I GGNCC 1 cut(s) 291
ScrFI CCNGG 2 cut(s) 11, 390
SduI GDGCHC 2 cut(s) 241, 390
SetI ASST 6 cut(s) 64, 74, 252, 306, 332, 372
SfaNI GCATC 2 cut(s) 156, 307
SfcI CTRYAG 1 cut(s) 242
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
SphI GCATGC 1 cut(s) 260
Sse9I AATT 2 cut(s) 155, 207
SsiI CCGC 4 cut(s) 47, 75, 78, 449
SspMI CTAG 1 cut(s) 197
StyD4I CCNGG 2 cut(s) 9, 388
TaaI ACNGT 1 cut(s) 466
TaqI TCGA 1 cut(s) 143
TasI AATT 2 cut(s) 155, 207
TauI GCSGC 2 cut(s) 80, 452
TfiI GAWTC 1 cut(s) 410
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TseI GCWGC 5 cut(s) 125, 193, 247, 320, 393
TspDTI ATGAA 1 cut(s) 104
VspI ATTAAT 1 cut(s) 32
XagI CCTNNNNNAGG 1 cut(s) 365
XapI RAATTY 1 cut(s) 155
XceI RCATGY 1 cut(s) 260
XmiI GTMKAC 1 cut(s) 134
XmnI GAANNNNTTC 1 cut(s) 108
XspI CTAG 1 cut(s) 197
Zsp2I ATGCAT 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.