Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0014317

Physical Location & Seq

Genomic Coordinates Reverse (-)

11666497 .. 11671559

Gene Structure 5063 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1148600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1968 bp

ATGGCGGTAGTGCCTCTGTTCGTCACGGCGACGAAGCTCGCCGGGCTACTAGTGACACTCACAGTCGCCGCCAATGCCTACTCTTATTCACGCTTTTGGAACAAGAATCTCCGCCCGTTCCAGTCTCCGATCGACGATTCCGACGACGAGGACGACGACACTCTCGCTGACTTCAATCTCCACGAAGGGGGAGATGAGTTTTTCTTCGGGTTGGCAACGGCTCCAGCCCATGTTGAGGACAGGCTAGATGATGCTTGGCTCCAGTTTGCAAAAGAAAATCCTTCTGACCAATCAGAGTCACAGGGGAGCCTGCAAACAGCAGATGCTATAATGGGTTCTGCTACTGGTGATGGAGGATCTCAGCCAGTTTCCTTACCAGGAAAAGCTGGGAAAACAGATAAGAAAAGGAAGCCTCTTAAGATAGCTATGGAAGCCATGATTAGAGGGTATGAGAAGTATATTGAAGGAGACGGAGGAGAAGAAGAAGTAGAAACACCTGTCCCAAACGAAGAATCCCATCATAATGTAGCTGCATGGCACAACGTTGTGCACCCAGAAGAGAGGCTAAGGTTTTGGTCTGATCCTGATACAGAACTAAAATTGGCTAAAGATACTGGAATCAGTGTCTTCCGAATGGGAATAGATTGGTCACGAATCATGCCAGAAGAACCACTCAATGGGCTAAAAGAAACTGTCAATTATGCAGCGTTGGAGCGATATAAGTGGATAATCAGCAGGGTCCGTTCATATGGAATGAAGGTGATGTTAACTCTGTTTCATCACTCATTACCACCATGGGCTGGAGAGTATGGAGGTTGGAAAATGGAAAAAACGGTTGATTATTTCATGGACTTTACAAAGCTTGTTGTTGACAGTGTCTCAGATATGATAGACTATTGGGTAACATTTAATGAGCCTCACGTCTTCTGTATGCTTACCTATTGTGCGGGTGCTTGGCCTGGTGGTCATCCTGACATGCTGGAGGCGGCCACTTCTGCTCTACCTACTGGTGTTTTCCAACAGGCTATGCATTGGATGGCCATTGCACACTCAAAGGCCTATGAATATATTCATGAACAAAGGTCTTCATCAAAACCAGTAGTTGGCGTGGCACACCATGTCTCATTTATGCGGCCATATGGTCTCTTTGATGTTGCTGCTGTTTCACTGGCGAACTCCCTGACTATGTACTCATATGTGGATAGTGTTTCTGACAAGCTGGATTTTATAGGCATAAACTACTATGGACAGGAAGTGGTGTGTGGTGCTGGACTGAAGCTAGTAGAGACTGATGAGTATAGTGAATCTGGACGTGGGGTATATCCAGATGGCTTGTACCGTGTGCTTCTTCAGTTCCATGAAAAATATAAACATCTTAATGTACCCTTCATGATTACTGAAAATGGGGTAGCCGATGAGACAGATTTGATCCGTCGGCCATATCTGCTAGAGCATTTGCTTTCTGTTTTTGCAGCCAAGACCAAGGGTGTCCCTGTGCTTGGCTACCTGTTTTGGACTATTTCTGATAACTGGGAGTGGGCAGATGGATATGGTCCCAAGTTTGGACTTGTAGCAGTTGAACGTGCCAATAATCTTGCCCGGATTCCACGTCCTTCTTACCATCTATTTACAAAGGTGGTGACAACAGGTAAAATTACACGTGATGACCGGGCACAAGCTTGGAATGACCTCAATCAAGCTGCTAAAGCAAAAAGAACACGACCATTTTATCGGGAAGTTAATGAGCATGGTTTGATGTATGCAGGAGGCCTTGATGAACCGATACAGCGGCCGTATACTGACAGAGATTGGCGATTTGGACACTACGAGATGGACGGTCTCCAGGACCCCTTAAGTCGCTTCTCAAGATTCATTCTCCGACCTTTCTCCTTCCTCAAAAGGAAACCTAAAAAGAAGAAATCATTTACCAGTAAGGAGAAGGTAACAAGCCTGACCCTGGTGCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0008150 GO:0008152 GO:0008378 GO:0008422 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009628 GO:0009707 GO:0009941 GO:0015926 GO:0016020 GO:0016021 GO:0016740 GO:0016757 GO:0016758 GO:0016787 GO:0016798 GO:0019867 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031350 GO:0031351 GO:0031354 GO:0031355 GO:0031358 GO:0031359 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050826 GO:0050896 GO:0071704 GO:0080079 GO:0098588 GO:0098805 GO:1901135 GO:1901657

KEGG Pathways

Metabolic & Signaling

ko00561 map00561

Pfam Domains

Protein Families

Glyco_hydro_1

Protein Analysis

656

Amino Acids

74.23

Weight (kDa)

5.76

Isoelectric Point (pI)

35.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_1	PF00232	196 - 381	1.7e-32	Glycosyl hydrolase family 1
Glyco_hydro_1	PF00232	403 - 549	1.2e-19	Glycosyl hydrolase family 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014317)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06510 AT3G06510
fragaria_vesca	FvH4_4g20710
malus_domestica	MD16G1148600.v1.1
prunus_persica	Prupe.1G112100_v2.0.a1 Prupe.1G112100_v2.0.a1 Prupe.1G112100_v2.0.a1
pyrus_communis	pycom16g12690
rosa_chinensis	RchiOBHm_Chr4g0426131
rosa_laevigata	RLG00000007317
rosa_multiflora	Rmu_co8494203.1_g000001
rosa_roxburghii	Rroxscaffold_5G00368120
rosa_rugosa	Rorug04G0209300
rosa_samantha	Rh4AG265800 Rh4CG284800 Rh4DG268000
rosa_wichuraiana	Rw4G022970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	3 cut(s)	677, 800, 1103
AccI	GTMKAC	1 cut(s)	1796
AciI	CCGC	7 cut(s)	5, 69, 112, 947, 986, 1132, 1789
AclI	AACGTT	1 cut(s)	543
AclWI	GGATC	3 cut(s)	364, 575, 1423
AcoI	YGGCCR	5 cut(s)	987, 1038, 1133, 1436, 1790
AcuI	CTGAAG	2 cut(s)	1295, 1334
AcvI	CACGTG	1 cut(s)	1661
AfaI	GTAC	3 cut(s)	1190, 1337, 1383
AfiI	CCNNNNNNNGG	8 cut(s)	187, 235, 677, 800, 1103, 1499, 1562, 1957
AflII	CTTAAG	2 cut(s)	416, 1852
AflIII	ACRYGT	1 cut(s)	1658
AgsI	TTSAA	3 cut(s)	175, 464, 1580
AhlI	ACTAGT	1 cut(s)	49
AjiI	CACGTC	3 cut(s)	922, 1313, 1610
AjnI	CCWGG	4 cut(s)	376, 958, 1842, 1956
AluBI	AGCT	9 cut(s)	37, 386, 425, 530, 862, 1219, 1279, 1679, 1700
AluI	AGCT	9 cut(s)	37, 386, 425, 530, 862, 1219, 1279, 1679, 1700
Alw21I	GWGCWC	1 cut(s)	552
Alw26I	GTCTC	8 cut(s)	129, 462, 883, 1126, 1148, 1280, 1412, 1844
Alw44I	GTGCAC	1 cut(s)	548
AlwI	GGATC	3 cut(s)	364, 575, 1423
AoxI	GGCC	8 cut(s)	956, 987, 1038, 1056, 1133, 1436, 1768, 1790
ApaLI	GTGCAC	1 cut(s)	548
ApeKI	GCWGC	5 cut(s)	530, 704, 1157, 1472, 1700
Asp700I	GAANNNNTTC	1 cut(s)	1068
AspS9I	GGNCC	3 cut(s)	739, 1553, 1846
AsuC2I	CCSGG	3 cut(s)	43, 1600, 1670
AsuHPI	GGTGA	3 cut(s)	359, 772, 1651
AvaII	GGWCC	3 cut(s)	739, 1553, 1846
BaeGI	GKGCMC	2 cut(s)	552, 1675
BalI	TGGCCA	1 cut(s)	1040
BbrPI	CACGTG	1 cut(s)	1661
BbsI	GAAGAC	3 cut(s)	619, 916, 1077
Bbv12I	GWGCWC	1 cut(s)	552
BbvI	GCAGC	5 cut(s)	517, 716, 1144, 1484, 1687
BccI	CCATC	7 cut(s)	344, 525, 1030, 1322, 1538, 1629, 1825
BceAI	ACGGC	3 cut(s)	42, 234, 1777
BciT130I	CCWGG	4 cut(s)	378, 960, 1844, 1958
BcnI	CCSGG	3 cut(s)	43, 1600, 1670
BcoDI	GTCTC	8 cut(s)	129, 462, 883, 1126, 1148, 1280, 1412, 1844
BcuI	ACTAGT	1 cut(s)	49
BfaI	CTAG	4 cut(s)	50, 245, 1280, 1448
BfrI	CTTAAG	2 cut(s)	416, 1852
BisI	GCNGC	9 cut(s)	69, 531, 705, 987, 1133, 1158, 1473, 1701, 1790
BlsI	GCNGC	9 cut(s)	70, 532, 706, 988, 1134, 1159, 1474, 1702, 1791
Bme1390I	CCNGG	7 cut(s)	43, 378, 960, 1600, 1670, 1844, 1958
Bme18I	GGWCC	3 cut(s)	739, 1553, 1846
BmgBI	CACGTC	3 cut(s)	922, 1313, 1610
BmgT120I	GGNCC	3 cut(s)	739, 1553, 1846
BmiI	GGNNCC	6 cut(s)	222, 260, 308, 740, 1555, 1848
BmrFI	CCNGG	7 cut(s)	43, 378, 960, 1600, 1670, 1844, 1958
BmrI	ACTGGG	1 cut(s)	1540
BmsI	GCATC	2 cut(s)	241, 313
BmuI	ACTGGG	1 cut(s)	1540
BpiI	GAAGAC	3 cut(s)	619, 916, 1077
BpmI	CTGGAG	5 cut(s)	207, 245, 822, 1001, 1826
Bpu10I	CCTNAGC	1 cut(s)	566
BpuEI	CTTGAG	1 cut(s)	1849
BpuMI	CCSGG	3 cut(s)	43, 1600, 1670
BsaAI	YACGTR	1 cut(s)	1661
BsaI	GGTCTC	2 cut(s)	1148, 1844
BsaJI	CCNNGG	3 cut(s)	794, 1482, 1956
Bsc4I	CCNNNNNNNGG	8 cut(s)	187, 235, 677, 800, 1103, 1499, 1562, 1957
Bse3DI	GCAATG	1 cut(s)	1041
BseBI	CCWGG	4 cut(s)	378, 960, 1844, 1958
BseDI	CCNNGG	3 cut(s)	794, 1482, 1956
BseGI	GGATG	2 cut(s)	967, 1041
BseLI	CCNNNNNNNGG	8 cut(s)	187, 235, 677, 800, 1103, 1499, 1562, 1957
BseMI	GCAATG	1 cut(s)	1041
BseMII	CTCAG	2 cut(s)	374, 894
BseRI	GAGGAG	1 cut(s)	489
BseSI	GKGCMC	2 cut(s)	552, 1675
BseX3I	CGGCCG	1 cut(s)	1790
BseXI	GCAGC	5 cut(s)	517, 716, 1144, 1484, 1687
BseYI	CCCAGC	1 cut(s)	386
Bsh1285I	CGRYCG	2 cut(s)	132, 1793
BshFI	GGCC	8 cut(s)	958, 989, 1040, 1058, 1135, 1438, 1770, 1792
BsiEI	CGRYCG	2 cut(s)	132, 1793
BsiHKAI	GWGCWC	1 cut(s)	552
BsiSI	CCGG	3 cut(s)	42, 1600, 1669
BslFI	GGGAC	3 cut(s)	485, 1475, 1539
BslI	CCNNNNNNNGG	8 cut(s)	187, 235, 677, 800, 1103, 1499, 1562, 1957
BsmAI	GTCTC	8 cut(s)	129, 462, 883, 1126, 1148, 1280, 1412, 1844
BsmBI	CGTCTC	1 cut(s)	462
BsmFI	GGGAC	3 cut(s)	485, 1475, 1539
BsnI	GGCC	8 cut(s)	958, 989, 1040, 1058, 1135, 1438, 1770, 1792
Bso31I	GGTCTC	2 cut(s)	1148, 1844
Bsp1286I	GDGCHC	2 cut(s)	552, 1675
Bsp143I	GATC	4 cut(s)	129, 356, 580, 1428
Bsp19I	CCATGG	1 cut(s)	794
BspACI	CCGC	7 cut(s)	5, 69, 112, 947, 986, 1132, 1789
BspANI	GGCC	8 cut(s)	958, 989, 1040, 1058, 1135, 1438, 1770, 1792
BspCNI	CTCAG	2 cut(s)	373, 893
BspHI	TCATGA	2 cut(s)	1072, 1389
BspLI	GGNNCC	6 cut(s)	222, 260, 308, 740, 1555, 1848
BspPI	GGATC	3 cut(s)	364, 575, 1423
BspTI	CTTAAG	2 cut(s)	416, 1852
BspTNI	GGTCTC	2 cut(s)	1148, 1844
BsrDI	GCAATG	1 cut(s)	1041
BssECI	CCNNGG	3 cut(s)	794, 1482, 1956
BssMI	GATC	4 cut(s)	129, 356, 580, 1428
BssNAI	GTATAC	1 cut(s)	1797
BssT1I	CCWWGG	2 cut(s)	794, 1482
Bst1107I	GTATAC	1 cut(s)	1797
Bst2UI	CCWGG	4 cut(s)	378, 960, 1844, 1958
Bst4CI	ACNGT	6 cut(s)	64, 694, 835, 875, 1340, 1838
Bst6I	CTCTTC	1 cut(s)	552
BstAFI	CTTAAG	2 cut(s)	416, 1852
BstBAI	YACGTR	1 cut(s)	1661
BstC8I	GCNNGC	2 cut(s)	39, 311
BstDEI	CTNAG	3 cut(s)	360, 566, 880
BstDSI	CCRYGG	1 cut(s)	794
BstF5I	GGATG	2 cut(s)	967, 1041
BstKTI	GATC	4 cut(s)	132, 359, 583, 1431
BstMAI	GTCTC	8 cut(s)	129, 462, 883, 1126, 1148, 1280, 1412, 1844
BstMBI	GATC	4 cut(s)	129, 356, 580, 1428
BstMCI	CGRYCG	2 cut(s)	132, 1793
BstMWI	GCNNNNNNNGC	6 cut(s)	43, 74, 431, 995, 1444, 1706
BstNI	CCWGG	4 cut(s)	378, 960, 1844, 1958
BstNSI	RCATGY	1 cut(s)	979
BstSCI	CCNGG	7 cut(s)	41, 376, 958, 1598, 1668, 1842, 1956
BstSLI	GKGCMC	2 cut(s)	552, 1675
BstV1I	GCAGC	5 cut(s)	517, 716, 1144, 1484, 1687
BstV2I	GAAGAC	3 cut(s)	619, 916, 1077
BstX2I	RGATCY	1 cut(s)	356
BstYI	RGATCY	1 cut(s)	356
BstZ17I	GTATAC	1 cut(s)	1797
BstZI	CGGCCG	1 cut(s)	1790
BsuRI	GGCC	8 cut(s)	958, 989, 1040, 1058, 1135, 1438, 1770, 1792
BtgI	CCRYGG	1 cut(s)	794
BtrI	CACGTC	3 cut(s)	922, 1313, 1610
BtsCI	GGATG	2 cut(s)	967, 1041
BtsIMutI	CAGTG	3 cut(s)	628, 880, 1166
Cac8I	GCNNGC	2 cut(s)	39, 311
CciI	TCATGA	2 cut(s)	1072, 1389
Cfr13I	GGNCC	3 cut(s)	739, 1553, 1846
Csp6I	GTAC	3 cut(s)	1189, 1336, 1382
CviQI	GTAC	3 cut(s)	1189, 1336, 1382
DdeI	CTNAG	3 cut(s)	360, 566, 880
DpnI	GATC	4 cut(s)	131, 358, 582, 1430
DpnII	GATC	4 cut(s)	129, 356, 580, 1428
EaeI	YGGCCR	5 cut(s)	987, 1038, 1133, 1436, 1790
EagI	CGGCCG	1 cut(s)	1790
Eam1104I	CTCTTC	1 cut(s)	552
EarI	CTCTTC	1 cut(s)	552
EciI	GGCGGA	1 cut(s)	101
EclXI	CGGCCG	1 cut(s)	1790
Eco130I	CCWWGG	2 cut(s)	794, 1482
Eco147I	AGGCCT	2 cut(s)	1058, 1770
Eco31I	GGTCTC	2 cut(s)	1148, 1844
Eco47I	GGWCC	3 cut(s)	739, 1553, 1846
Eco52I	CGGCCG	1 cut(s)	1790
Eco57I	CTGAAG	2 cut(s)	1295, 1334
Eco72I	CACGTG	1 cut(s)	1661
EcoO109I	RGGNCCY	1 cut(s)	1846
EcoRII	CCWGG	4 cut(s)	376, 958, 1842, 1956
EcoT14I	CCWWGG	2 cut(s)	794, 1482
EcoT22I	ATGCAT	1 cut(s)	1032
ErhI	CCWWGG	2 cut(s)	794, 1482
Esp3I	CGTCTC	1 cut(s)	462
FaqI	GGGAC	3 cut(s)	485, 1475, 1539
FauI	CCCGC	1 cut(s)	940
FauNDI	CATATG	3 cut(s)	748, 1138, 1195
FblI	GTMKAC	1 cut(s)	1796
Fnu4HI	GCNGC	9 cut(s)	69, 531, 705, 987, 1133, 1158, 1473, 1701, 1790
FokI	GGATG	2 cut(s)	954, 1048
Fsp4HI	GCNGC	9 cut(s)	69, 531, 705, 987, 1133, 1158, 1473, 1701, 1790
FspBI	CTAG	4 cut(s)	50, 245, 1280, 1448
GluI	GCNGC	9 cut(s)	69, 531, 705, 987, 1133, 1158, 1473, 1701, 1790
GsaI	CCCAGC	1 cut(s)	390
GsuI	CTGGAG	5 cut(s)	207, 245, 822, 1001, 1826
HaeIII	GGCC	8 cut(s)	958, 989, 1040, 1058, 1135, 1438, 1770, 1792
HapII	CCGG	3 cut(s)	42, 1600, 1669
HincII	GTYRAC	2 cut(s)	768, 871
HindII	GTYRAC	2 cut(s)	768, 871
HindIII	AAGCTT	2 cut(s)	860, 1677
HinfI	GANTC	9 cut(s)	106, 137, 296, 512, 618, 654, 1304, 1603, 1869
HpaI	GTTAAC	1 cut(s)	768
HpaII	CCGG	3 cut(s)	42, 1600, 1669
HphI	GGTGA	3 cut(s)	359, 772, 1651
Hpy166II	GTNNAC	4 cut(s)	550, 768, 871, 1797
Hpy188III	TCNNGA	9 cut(s)	584, 651, 971, 1073, 1308, 1325, 1390, 1733, 1866
Hpy8I	GTNNAC	4 cut(s)	550, 768, 871, 1797
Hpy99I	CGWCG	6 cut(s)	34, 137, 146, 149, 158, 1437
HpyAV	CCTTC	8 cut(s)	179, 291, 458, 751, 1396, 1623, 1900, 1933
HpyCH4III	ACNGT	6 cut(s)	64, 694, 835, 875, 1340, 1838
HpyCH4IV	ACGT	6 cut(s)	543, 921, 1312, 1582, 1609, 1660
HpyF10VI	GCNNNNNNNGC	6 cut(s)	43, 74, 431, 995, 1444, 1706
HpyF3I	CTNAG	3 cut(s)	360, 566, 880
HpySE526I	ACGT	6 cut(s)	543, 921, 1312, 1582, 1609, 1660
KspAI	GTTAAC	1 cut(s)	768
Kzo9I	GATC	4 cut(s)	129, 356, 580, 1428
LmnI	GCTCC	4 cut(s)	226, 264, 306, 712
Lsp1109I	GCAGC	5 cut(s)	517, 716, 1144, 1484, 1687
LweI	GCATC	2 cut(s)	241, 313
MaeI	CTAG	4 cut(s)	50, 245, 1280, 1448
MaeII	ACGT	6 cut(s)	543, 921, 1312, 1582, 1609, 1660
MaeIII	GTNAC	7 cut(s)	22, 52, 297, 648, 901, 1639, 1942
MalI	GATC	4 cut(s)	131, 358, 582, 1430
MboI	GATC	4 cut(s)	129, 356, 580, 1428
MflI	RGATCY	1 cut(s)	356
MhlI	GDGCHC	2 cut(s)	552, 1675
MlsI	TGGCCA	1 cut(s)	1040
MluCI	AATT	3 cut(s)	599, 697, 1653
MluNI	TGGCCA	1 cut(s)	1040
MlyI	GAGTC	1 cut(s)	305
MmeI	TCCRAC	5 cut(s)	165, 690, 797, 1042, 1903
Mox20I	TGGCCA	1 cut(s)	1040
Mph1103I	ATGCAT	1 cut(s)	1032
MroXI	GAANNNNTTC	1 cut(s)	1068
MscI	TGGCCA	1 cut(s)	1040
MseI	TTAA	6 cut(s)	417, 767, 909, 1377, 1740, 1853
MslI	CAYNNNNRTG	2 cut(s)	522, 1377
Msp20I	TGGCCA	1 cut(s)	1040
MspA1I	CMGCKG	1 cut(s)	1789
MspCI	CTTAAG	2 cut(s)	416, 1852
MspI	CCGG	3 cut(s)	42, 1600, 1669
MspR9I	CCNGG	7 cut(s)	43, 378, 960, 1600, 1670, 1844, 1958
MvaI	CCWGG	4 cut(s)	378, 960, 1844, 1958
MwoI	GCNNNNNNNGC	6 cut(s)	43, 74, 431, 995, 1444, 1706
NciI	CCSGG	3 cut(s)	43, 1600, 1670
NcoI	CCATGG	1 cut(s)	794
NdeI	CATATG	3 cut(s)	748, 1138, 1195
NdeII	GATC	4 cut(s)	129, 356, 580, 1428
NlaIV	GGNNCC	6 cut(s)	222, 260, 308, 740, 1555, 1848
NmuCI	GTSAC	5 cut(s)	22, 52, 297, 648, 1639
NsiI	ATGCAT	1 cut(s)	1032
NspI	RCATGY	1 cut(s)	979
PagI	TCATGA	2 cut(s)	1072, 1389
PceI	AGGCCT	2 cut(s)	1058, 1770
PcsI	WCGNNNNNNNCGW	4 cut(s)	138, 141, 150, 153
PdmI	GAANNNNTTC	1 cut(s)	1068
PfeI	GAWTC	8 cut(s)	106, 137, 512, 618, 654, 1304, 1603, 1869
PflFI	GACNNNGTC	1 cut(s)	875
PflMI	CCANNNNNTGG	3 cut(s)	677, 800, 1103
PfoI	TCCNGGA	1 cut(s)	1842
PkrI	GCNGC	9 cut(s)	70, 532, 706, 988, 1134, 1159, 1474, 1702, 1791
Ple19I	CGATCG	1 cut(s)	132
PleI	GAGTC	1 cut(s)	304
PmaCI	CACGTG	1 cut(s)	1661
PmlI	CACGTG	1 cut(s)	1661
PpsI	GAGTC	1 cut(s)	304
Ppu21I	YACGTR	1 cut(s)	1661
PpuMI	RGGWCCY	1 cut(s)	1846
Psp1406I	AACGTT	1 cut(s)	543
Psp5II	RGGWCCY	1 cut(s)	1846
Psp6I	CCWGG	4 cut(s)	376, 958, 1842, 1956
PspCI	CACGTG	1 cut(s)	1661
PspFI	CCCAGC	1 cut(s)	386
PspGI	CCWGG	4 cut(s)	376, 958, 1842, 1956
PspN4I	GGNNCC	6 cut(s)	222, 260, 308, 740, 1555, 1848
PspPI	GGNCC	3 cut(s)	739, 1553, 1846
PspPPI	RGGWCCY	1 cut(s)	1846
PsuI	RGATCY	1 cut(s)	356
PsyI	GACNNNGTC	1 cut(s)	875
PvuI	CGATCG	1 cut(s)	132
RsaI	GTAC	3 cut(s)	1190, 1337, 1383
RsaNI	GTAC	3 cut(s)	1189, 1336, 1382
RseI	CAYNNNNRTG	2 cut(s)	522, 1377
SaqAI	TTAA	6 cut(s)	417, 767, 909, 1377, 1740, 1853
SatI	GCNGC	9 cut(s)	69, 531, 705, 987, 1133, 1158, 1473, 1701, 1790
Sau3AI	GATC	4 cut(s)	129, 356, 580, 1428
Sau96I	GGNCC	3 cut(s)	739, 1553, 1846
SchI	GAGTC	1 cut(s)	305
ScrFI	CCNGG	7 cut(s)	43, 378, 960, 1600, 1670, 1844, 1958
SduI	GDGCHC	2 cut(s)	552, 1675
SfaNI	GCATC	2 cut(s)	241, 313
SinI	GGWCC	3 cut(s)	739, 1553, 1846
SmiMI	CAYNNNNRTG	2 cut(s)	522, 1377
SmlI	CTYRAG	3 cut(s)	416, 1852, 1864
SmoI	CTYRAG	3 cut(s)	416, 1852, 1864
SpeI	ACTAGT	1 cut(s)	49
Sse9I	AATT	3 cut(s)	599, 697, 1653
SseBI	AGGCCT	2 cut(s)	1058, 1770
SsiI	CCGC	7 cut(s)	5, 69, 112, 947, 986, 1132, 1789
SspMI	CTAG	4 cut(s)	50, 245, 1280, 1448
StuI	AGGCCT	2 cut(s)	1058, 1770
StyD4I	CCNGG	7 cut(s)	41, 376, 958, 1598, 1668, 1842, 1956
StyI	CCWWGG	2 cut(s)	794, 1482
TaaI	ACNGT	6 cut(s)	64, 694, 835, 875, 1340, 1838
TaiI	ACGT	6 cut(s)	546, 924, 1315, 1585, 1612, 1663
TaqI	TCGA	1 cut(s)	132
TasI	AATT	3 cut(s)	599, 697, 1653
TatI	WGTACW	1 cut(s)	1188
TauI	GCSGC	4 cut(s)	71, 989, 1135, 1792
TfiI	GAWTC	8 cut(s)	106, 137, 512, 618, 654, 1304, 1603, 1869
Tru1I	TTAA	6 cut(s)	417, 767, 909, 1377, 1740, 1853
Tru9I	TTAA	6 cut(s)	417, 767, 909, 1377, 1740, 1853
TscAI	CASTG	3 cut(s)	628, 880, 1173
TseFI	GTSAC	5 cut(s)	22, 52, 297, 648, 1639
TseI	GCWGC	5 cut(s)	530, 704, 1157, 1472, 1700
Tsp45I	GTSAC	5 cut(s)	22, 52, 297, 648, 1639
TspGWI	ACGGA	3 cut(s)	486, 731, 1421
TspRI	CASTG	3 cut(s)	628, 880, 1173
Tth111I	GACNNNGTC	1 cut(s)	875
Van91I	CCANNNNNTGG	3 cut(s)	677, 800, 1103
Vha464I	CTTAAG	2 cut(s)	416, 1852
VneI	GTGCAC	1 cut(s)	548
VpaK11BI	GGWCC	3 cut(s)	739, 1553, 1846
XceI	RCATGY	1 cut(s)	979
XmiI	GTMKAC	1 cut(s)	1796
XmnI	GAANNNNTTC	1 cut(s)	1068
XspI	CTAG	4 cut(s)	50, 245, 1280, 1448
Zsp2I	ATGCAT	1 cut(s)	1032

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.