Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0014317

Physical Location & Seq

Genomic Coordinates Reverse (-)

48454497 .. 48459174

Gene Structure 4678 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G022970.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1935 bp

ATGGCGGTAGTACCTCTCTTCGTCACGGCAACGAAGCTCGCCGGATTATTGGCCACCCTCACCGTCGCTGCCAATGTCTTCTCTTTCTCACGCTACCGGAAGAAGAATCTCCGGCCGTTCCGCTCCCCGATCGACGAGTCCTCCGACCCTCTCGCCGACTTCAATCTCAACGAAGGGGAAGATGGGTTCTTCTTTGGATTGGCAACGGCTCCAGCGCACGTTGAAGACGGGCTCAATGATGCTTGGCTTCAGTTTGCGCAAGAAAATCCTTGTGACAAATCAGAGTCTCAAGGTGATCGGCAGACAGCTGATGCTATAATGGGTTCCGCCACTGGCGATGGTGGGTCTCAGACAGCTCCCCCATTGGGAATTCCTAAGAAAAAGAAGCCTCCTAAGATAGCTATGGAAGCCATGATTAGAGGGTATGCAAAGTATATTAAAGGGGACACAGGAGAAGAAGAAGAAGAAAAAAAACCTGTCCCAAATGAAGAATGCCATCATAATGTAGCTGCATGGCACAATGTTGAGCACCCAGAGGAGAGGATAAAGTTTTGGTCCGATCCTGATACAGAGCTAAAACTGGCTAAAGATACTGGCATCAGTGTCTTCCGAATGGGAATAGATTGGTCACGAGTCATGCCTGAGGAGCCGCTCAATGGGCTTACAAAAACTGTTAACTATGCTGCATTGGAGCGATACAAGTGGATCATCGGCAGGGTTCGCTCATACGGAATGAAGGTGATGTTGACTCTCTTCCATCACTCATTGCCACCATGGGCTGGGGAGTATGGAGGGTGGAAAATGGAAAAGACAGTTGATTATTTCTTGGATTTCACAAGGCTTGTTGTTGACAGTGTATCAGATATGATAGACTATTGGGTACCATTCAATGAGCCTCATGTCTTCTGTATGCTTACCTATTGTGCGGGTGCATGGCCTGGTGGCCATCCTGACATGCTGGAGGTTGCGACTTCTGCTCTACCAACTGGTGTTTTCAAACAGGCCATGCATTGGATGGCCATTGCACACTTAAAGGCCTATGACTATATCCACGAAAAAAGTACCTCATCCAAGCCATTAGTTGGAGTGGCACACCATGTCTCATTCATGCGGCCATATGGTCTCTTTGATGTTCCTGCTGTTACACTGGCCAACTCCATGACTGTCTTCCCATATGTGGATAGTATTTCTGACAAGCTGGATTTTATTGGCATAAACTACTATGGACAGGAAGTGGTGTGTGGTGCTGGACTGAAGCTAGTAGAGACTGATGAATATAGTGAATCCGGACGTGGGATATACCCAGATGGCTTGTACCGCATGCTGCTTCAGTTCAATGACAGATATAAACATCTAAATGTACCCTTCATAATTACTGAAAATGGGGTATCTGATGAGACAGATGTAATCCGTCGGCCTTACCTGCTAGAGCATTTACTCGCTGTTCATGCAGCCAAGATCATGGGTGTCCGTGTGCTTGGTTTCCTGTTTTGGACTATTTCTGATAACTGGGAGTGGGCAGATGGGTATGGTCCCAAGTTTGGACTGGTAGCAGTTGATCGGGCCAAAGATCTTGCCCGGGTTCCACGTCCTTCATACCATCTATTTACTAAGGTGGTGACAACAGGTAAAATTACACGTTATGACCGGGCACGTGCATGGCACCAACTCCAAATATCTGCTAGAGAAAAGAAAATGCGATCCTTTTATCGGGCTGTTAATAAAGATGGTTTGATGTATGCAGGAGGCCTTGATGAACCAATACAGAGACCCTATGTTGAAAGAGATTGGCGATTCGGACACTATGAGATGGAAGGTCTTCAGGACCCCATTTGTCGCTTGTGGAGATTCATGACCCGACCTGCCACCCTAAAATTTAGGCAAAGGAAACCCCAGATGGATGAAGAGGATGAAGCTCTATTGGCTCTCCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0008150 GO:0008152 GO:0008378 GO:0008422 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009628 GO:0009707 GO:0009941 GO:0015926 GO:0016020 GO:0016021 GO:0016740 GO:0016757 GO:0016758 GO:0016787 GO:0016798 GO:0019867 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031350 GO:0031351 GO:0031354 GO:0031355 GO:0031358 GO:0031359 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050826 GO:0050896 GO:0071704 GO:0080079 GO:0098588 GO:0098805 GO:1901135 GO:1901657

KEGG Pathways

Metabolic & Signaling

ko00561 map00561

Pfam Domains

Protein Families

Glyco_hydro_1

Protein Analysis

644

Amino Acids

73.23

Weight (kDa)

6.16

Isoelectric Point (pI)

35.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_1	PF00232	182 - 374	2e-29	Glycosyl hydrolase family 1
Glyco_hydro_1	PF00232	395 - 542	2.5e-19	Glycosyl hydrolase family 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014317)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06510 AT3G06510
fragaria_vesca	FvH4_4g20710
malus_domestica	MD16G1148600.v1.1
prunus_persica	Prupe.1G112100_v2.0.a1 Prupe.1G112100_v2.0.a1 Prupe.1G112100_v2.0.a1
pyrus_communis	pycom16g12690
rosa_chinensis	RchiOBHm_Chr4g0426131
rosa_laevigata	RLG00000007317
rosa_multiflora	Rmu_co8494203.1_g000001
rosa_roxburghii	Rroxscaffold_5G00368120
rosa_rugosa	Rorug04G0209300
rosa_samantha	Rh4AG265800 Rh4CG284800 Rh4DG268000
rosa_wichuraiana	Rw4G022970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	258
Acc36I	ACCTGC	2 cut(s)	1431, 1870
Acc65I	GGTACC	1 cut(s)	880
AccB1I	GGYRCC	2 cut(s)	880, 1662
AccB7I	CCANNNNNTGG	3 cut(s)	779, 1011, 1082
AccBSI	CCGCTC	2 cut(s)	123, 652
AccIII	TCCGGA	1 cut(s)	1286
AciI	CCGC	7 cut(s)	5, 121, 327, 650, 926, 1111, 1318
AclWI	GGATC	3 cut(s)	554, 713, 1695
AcoI	YGGCCR	6 cut(s)	51, 113, 943, 1017, 1112, 1149
AcsI	RAATTY	2 cut(s)	369, 1874
AcuI	CTGAAG	4 cut(s)	233, 1274, 1313, 1805
AcvI	CACGTG	1 cut(s)	1655
AfaI	GTAC	5 cut(s)	12, 882, 1063, 1316, 1362
AfiI	CCNNNNNNNGG	9 cut(s)	365, 656, 779, 1011, 1082, 1177, 1293, 1541, 1710
AflIII	ACRYGT	1 cut(s)	1637
AgsI	TTSAA	6 cut(s)	163, 224, 889, 997, 1336, 1781
AjiI	CACGTC	2 cut(s)	1292, 1589
AjnI	CCWGG	1 cut(s)	937
AloI	GAACNNNNNNTCC	2 cut(s)	170, 202
AluBI	AGCT	9 cut(s)	37, 308, 356, 401, 509, 574, 1198, 1258, 1916
AluI	AGCT	9 cut(s)	37, 308, 356, 401, 509, 574, 1198, 1258, 1916
Alw21I	GWGCWC	1 cut(s)	531
Alw26I	GTCTC	7 cut(s)	291, 351, 1105, 1127, 1259, 1391, 1762
AlwI	GGATC	3 cut(s)	554, 713, 1695
Ama87I	CYCGRG	1 cut(s)	1578
Aor13HI	TCCGGA	1 cut(s)	1286
ApeKI	GCWGC	5 cut(s)	68, 509, 683, 1324, 1451
ApoI	RAATTY	2 cut(s)	369, 1874
Asp700I	GAANNNNTTC	1 cut(s)	1818
Asp718I	GGTACC	1 cut(s)	880
AspLEI	GCGC	2 cut(s)	217, 259
AspS9I	GGNCC	4 cut(s)	555, 1532, 1563, 1825
AsuC2I	CCSGG	3 cut(s)	1579, 1580, 1649
AsuHPI	GGTGA	4 cut(s)	52, 305, 751, 1630
AvaI	CYCGRG	1 cut(s)	1578
AvaII	GGWCC	3 cut(s)	555, 1532, 1825
AxyI	CCTNAGG	1 cut(s)	642
BaeGI	GKGCMC	1 cut(s)	1654
BalI	TGGCCA	4 cut(s)	53, 945, 1019, 1151
BanI	GGYRCC	2 cut(s)	880, 1662
BanII	GRGCYC	1 cut(s)	234
BauI	CACGAG	1 cut(s)	630
BbrPI	CACGTG	1 cut(s)	1655
BbsI	GAAGAC	6 cut(s)	70, 231, 598, 895, 1159, 1811
Bbv12I	GWGCWC	1 cut(s)	531
BbvI	GCAGC	5 cut(s)	55, 496, 670, 1311, 1463
BceAI	ACGGC	3 cut(s)	42, 100, 222
BciT130I	CCWGG	1 cut(s)	939
BcnI	CCSGG	3 cut(s)	1579, 1580, 1649
BcoDI	GTCTC	7 cut(s)	291, 351, 1105, 1127, 1259, 1391, 1762
BfaI	CTAG	3 cut(s)	1259, 1427, 1683
BfuAI	ACCTGC	2 cut(s)	1431, 1870
BglII	AGATCT	1 cut(s)	1570
BisI	GCNGC	7 cut(s)	69, 510, 650, 684, 1112, 1325, 1452
BlsI	GCNGC	7 cut(s)	70, 511, 651, 685, 1113, 1326, 1453
Bme1390I	CCNGG	4 cut(s)	939, 1579, 1580, 1649
Bme18I	GGWCC	3 cut(s)	555, 1532, 1825
BmeT110I	CYCGRG	1 cut(s)	1578
BmgBI	CACGTC	2 cut(s)	1292, 1589
BmgT120I	GGNCC	4 cut(s)	555, 1532, 1563, 1825
BmiI	GGNNCC	8 cut(s)	210, 325, 648, 882, 1534, 1584, 1664, 1827
BmrFI	CCNGG	4 cut(s)	939, 1579, 1580, 1649
BmrI	ACTGGG	1 cut(s)	1519
BmsI	GCATC	3 cut(s)	229, 301, 606
BmuI	ACTGGG	1 cut(s)	1519
BpiI	GAAGAC	6 cut(s)	70, 231, 598, 895, 1159, 1811
BpmI	CTGGAG	3 cut(s)	195, 980, 1913
BpuEI	CTTGAG	1 cut(s)	273
BpuMI	CCSGG	3 cut(s)	1579, 1580, 1649
BsaAI	YACGTR	1 cut(s)	1655
BsaI	GGTCTC	3 cut(s)	351, 1127, 1762
BsaJI	CCNNGG	2 cut(s)	773, 1578
BsaWI	WCCGGW	2 cut(s)	96, 1286
BsaXI	ACNNNNNCTCC	2 cut(s)	125, 155
Bsc4I	CCNNNNNNNGG	9 cut(s)	365, 656, 779, 1011, 1082, 1177, 1293, 1541, 1710
Bse1I	ACTGG	8 cut(s)	337, 585, 598, 991, 1152, 1514, 1551, 1930
Bse21I	CCTNAGG	1 cut(s)	642
Bse3DI	GCAATG	2 cut(s)	764, 1020
BseAI	TCCGGA	1 cut(s)	1286
BseBI	CCWGG	1 cut(s)	939
BseDI	CCNNGG	2 cut(s)	773, 1578
BseGI	GGATG	5 cut(s)	946, 1020, 1067, 1906, 1915
BseLI	CCNNNNNNNGG	9 cut(s)	365, 656, 779, 1011, 1082, 1177, 1293, 1541, 1710
BseMI	GCAATG	2 cut(s)	764, 1020
BseMII	CTCAG	2 cut(s)	362, 633
BseNI	ACTGG	8 cut(s)	337, 585, 598, 991, 1152, 1514, 1551, 1930
BseRI	GAGGAG	2 cut(s)	551, 659
BseSI	GKGCMC	1 cut(s)	1654
BseX3I	CGGCCG	1 cut(s)	113
BseXI	GCAGC	5 cut(s)	55, 496, 670, 1311, 1463
BseYI	CCCAGC	1 cut(s)	779
Bsh1285I	CGRYCG	2 cut(s)	116, 132
BshNI	GGYRCC	2 cut(s)	880, 1662
BsiEI	CGRYCG	2 cut(s)	116, 132
BsiHKAI	GWGCWC	1 cut(s)	531
BsiHKCI	CYCGRG	1 cut(s)	1578
BsiSI	CCGG	6 cut(s)	42, 97, 112, 1287, 1579, 1648
BslFI	GGGAC	3 cut(s)	458, 464, 1518
BslI	CCNNNNNNNGG	9 cut(s)	365, 656, 779, 1011, 1082, 1177, 1293, 1541, 1710
BsmAI	GTCTC	7 cut(s)	291, 351, 1105, 1127, 1259, 1391, 1762
BsmFI	GGGAC	3 cut(s)	458, 464, 1518
BsmI	GAATGC	1 cut(s)	497
Bso31I	GGTCTC	3 cut(s)	351, 1127, 1762
BsoBI	CYCGRG	1 cut(s)	1578
Bsp1286I	GDGCHC	3 cut(s)	234, 531, 1654
Bsp13I	TCCGGA	1 cut(s)	1286
Bsp143I	GATC	8 cut(s)	129, 295, 559, 705, 1458, 1558, 1570, 1700
Bsp19I	CCATGG	1 cut(s)	773
BspACI	CCGC	7 cut(s)	5, 121, 327, 650, 926, 1111, 1318
BspCNI	CTCAG	2 cut(s)	361, 634
BspEI	TCCGGA	1 cut(s)	1286
BspHI	TCATGA	1 cut(s)	1851
BspLI	GGNNCC	8 cut(s)	210, 325, 648, 882, 1534, 1584, 1664, 1827
BspMI	ACCTGC	2 cut(s)	1431, 1870
BspPI	GGATC	3 cut(s)	554, 713, 1695
BspT107I	GGYRCC	2 cut(s)	880, 1662
BspTNI	GGTCTC	3 cut(s)	351, 1127, 1762
BsrBI	CCGCTC	2 cut(s)	123, 652
BsrDI	GCAATG	2 cut(s)	764, 1020
BsrI	ACTGG	8 cut(s)	337, 585, 598, 991, 1152, 1514, 1551, 1930
BssECI	CCNNGG	2 cut(s)	773, 1578
BssMI	GATC	8 cut(s)	129, 295, 559, 705, 1458, 1558, 1570, 1700
BssSI	CACGAG	1 cut(s)	630
BssT1I	CCWWGG	1 cut(s)	773
Bst2BI	CACGAG	1 cut(s)	630
Bst2UI	CCWGG	1 cut(s)	939
Bst4CI	ACNGT	5 cut(s)	64, 673, 814, 854, 1165
Bst6I	CTCTTC	3 cut(s)	23, 758, 1899
BstBAI	YACGTR	1 cut(s)	1655
BstC8I	GCNNGC	2 cut(s)	39, 1322
BstDEI	CTNAG	5 cut(s)	348, 375, 393, 642, 1611
BstDSI	CCRYGG	1 cut(s)	773
BstF5I	GGATG	5 cut(s)	946, 1020, 1067, 1906, 1915
BstHHI	GCGC	2 cut(s)	217, 259
BstKTI	GATC	8 cut(s)	132, 298, 562, 708, 1461, 1561, 1573, 1703
BstMAI	GTCTC	7 cut(s)	291, 351, 1105, 1127, 1259, 1391, 1762
BstMBI	GATC	8 cut(s)	129, 295, 559, 705, 1458, 1558, 1570, 1700
BstMCI	CGRYCG	2 cut(s)	116, 132
BstMWI	GCNNNNNNNGC	9 cut(s)	407, 646, 658, 720, 974, 1317, 1423, 1448, 1922
BstNI	CCWGG	1 cut(s)	939
BstNSI	RCATGY	2 cut(s)	958, 1324
BstSCI	CCNGG	4 cut(s)	937, 1577, 1578, 1647
BstSLI	GKGCMC	1 cut(s)	1654
BstV1I	GCAGC	5 cut(s)	55, 496, 670, 1311, 1463
BstV2I	GAAGAC	6 cut(s)	70, 231, 598, 895, 1159, 1811
BstX2I	RGATCY	1 cut(s)	1570
BstXI	CCANNNNNNTGG	1 cut(s)	1462
BstYI	RGATCY	1 cut(s)	1570
BstZI	CGGCCG	1 cut(s)	113
Bsu36I	CCTNAGG	1 cut(s)	642
BtgI	CCRYGG	1 cut(s)	773
BtgZI	GCGATG	1 cut(s)	351
BtrI	CACGTC	2 cut(s)	1292, 1589
BtsCI	GGATG	5 cut(s)	946, 1020, 1067, 1906, 1915
BtsIMutI	CAGTG	4 cut(s)	330, 607, 859, 1145
BveI	ACCTGC	2 cut(s)	1431, 1870
Cac8I	GCNNGC	2 cut(s)	39, 1322
CciI	TCATGA	1 cut(s)	1851
CfoI	GCGC	2 cut(s)	217, 259
Cfr13I	GGNCC	4 cut(s)	555, 1532, 1563, 1825
Cfr9I	CCCGGG	1 cut(s)	1578
Csp6I	GTAC	5 cut(s)	11, 881, 1062, 1315, 1361
CviQI	GTAC	5 cut(s)	11, 881, 1062, 1315, 1361
DdeI	CTNAG	5 cut(s)	348, 375, 393, 642, 1611
DpnI	GATC	8 cut(s)	131, 297, 561, 707, 1460, 1560, 1572, 1702
DpnII	GATC	8 cut(s)	129, 295, 559, 705, 1458, 1558, 1570, 1700
EaeI	YGGCCR	6 cut(s)	51, 113, 943, 1017, 1112, 1149
EagI	CGGCCG	1 cut(s)	113
Eam1104I	CTCTTC	3 cut(s)	23, 758, 1899
EarI	CTCTTC	3 cut(s)	23, 758, 1899
EciI	GGCGGA	1 cut(s)	316
EclXI	CGGCCG	1 cut(s)	113
Eco130I	CCWWGG	1 cut(s)	773
Eco147I	AGGCCT	2 cut(s)	1037, 1749
Eco24I	GRGCYC	1 cut(s)	234
Eco31I	GGTCTC	3 cut(s)	351, 1127, 1762
Eco47I	GGWCC	3 cut(s)	555, 1532, 1825
Eco52I	CGGCCG	1 cut(s)	113
Eco57I	CTGAAG	4 cut(s)	233, 1274, 1313, 1805
Eco72I	CACGTG	1 cut(s)	1655
Eco81I	CCTNAGG	1 cut(s)	642
Eco88I	CYCGRG	1 cut(s)	1578
EcoO109I	RGGNCCY	1 cut(s)	1825
EcoRI	GAATTC	1 cut(s)	369
EcoRII	CCWGG	1 cut(s)	937
EcoT14I	CCWWGG	1 cut(s)	773
EcoT22I	ATGCAT	1 cut(s)	1011
EcoT38I	GRGCYC	1 cut(s)	234
ErhI	CCWWGG	1 cut(s)	773
FaqI	GGGAC	3 cut(s)	458, 464, 1518
FauI	CCCGC	1 cut(s)	919
FauNDI	CATATG	2 cut(s)	1117, 1174
Fnu4HI	GCNGC	7 cut(s)	69, 510, 650, 684, 1112, 1325, 1452
FokI	GGATG	5 cut(s)	933, 1027, 1054, 1913, 1922
FriOI	GRGCYC	1 cut(s)	234
Fsp4HI	GCNGC	7 cut(s)	69, 510, 650, 684, 1112, 1325, 1452
FspBI	CTAG	3 cut(s)	1259, 1427, 1683
FspI	TGCGCA	1 cut(s)	258
GlaI	GCGC	2 cut(s)	216, 258
GluI	GCNGC	7 cut(s)	69, 510, 650, 684, 1112, 1325, 1452
GsaI	CCCAGC	1 cut(s)	783
GsuI	CTGGAG	3 cut(s)	195, 980, 1913
HapII	CCGG	6 cut(s)	42, 97, 112, 1287, 1579, 1648
HhaI	GCGC	2 cut(s)	217, 259
Hin6I	GCGC	2 cut(s)	215, 257
HinP1I	GCGC	2 cut(s)	215, 257
HincII	GTYRAC	3 cut(s)	676, 747, 850
HindII	GTYRAC	3 cut(s)	676, 747, 850
HinfI	GANTC	8 cut(s)	106, 137, 284, 633, 748, 1283, 1794, 1848
HpaI	GTTAAC	1 cut(s)	676
HpaII	CCGG	6 cut(s)	42, 97, 112, 1287, 1579, 1648
HphI	GGTGA	4 cut(s)	52, 305, 751, 1630
Hpy166II	GTNNAC	3 cut(s)	676, 747, 850
Hpy188III	TCNNGA	6 cut(s)	563, 630, 950, 1287, 1823, 1852
Hpy8I	GTNNAC	3 cut(s)	676, 747, 850
Hpy99I	CGWCG	3 cut(s)	68, 137, 1416
HpyAV	CCTTC	5 cut(s)	167, 730, 1375, 1602, 1808
HpyCH4III	ACNGT	5 cut(s)	64, 673, 814, 854, 1165
HpyCH4IV	ACGT	5 cut(s)	219, 1291, 1588, 1639, 1654
HpyCH4V	TGCA	9 cut(s)	428, 512, 686, 932, 1009, 1025, 1451, 1658, 1742
HpyF10VI	GCNNNNNNNGC	9 cut(s)	407, 646, 658, 720, 974, 1317, 1423, 1448, 1922
HpyF3I	CTNAG	5 cut(s)	348, 375, 393, 642, 1611
HpySE526I	ACGT	5 cut(s)	219, 1291, 1588, 1639, 1654
HspAI	GCGC	2 cut(s)	215, 257
Kpn2I	TCCGGA	1 cut(s)	1286
KpnI	GGTACC	1 cut(s)	884
KspAI	GTTAAC	1 cut(s)	676
Kzo9I	GATC	8 cut(s)	129, 295, 559, 705, 1458, 1558, 1570, 1700
LmnI	GCTCC	5 cut(s)	128, 214, 361, 646, 691
Lsp1109I	GCAGC	5 cut(s)	55, 496, 670, 1311, 1463
LweI	GCATC	3 cut(s)	229, 301, 606
MaeI	CTAG	3 cut(s)	1259, 1427, 1683
MaeII	ACGT	5 cut(s)	219, 1291, 1588, 1639, 1654
MaeIII	GTNAC	5 cut(s)	22, 272, 627, 1141, 1618
MalI	GATC	8 cut(s)	131, 297, 561, 707, 1460, 1560, 1572, 1702
MbiI	CCGCTC	2 cut(s)	123, 652
MboI	GATC	8 cut(s)	129, 295, 559, 705, 1458, 1558, 1570, 1700
MflI	RGATCY	1 cut(s)	1570
MhlI	GDGCHC	3 cut(s)	234, 531, 1654
MlsI	TGGCCA	4 cut(s)	53, 945, 1019, 1151
MluCI	AATT	4 cut(s)	369, 1371, 1632, 1874
MluNI	TGGCCA	4 cut(s)	53, 945, 1019, 1151
MlyI	GAGTC	4 cut(s)	146, 293, 642, 742
MmeI	TCCRAC	2 cut(s)	168, 1063
Mox20I	TGGCCA	4 cut(s)	53, 945, 1019, 1151
Mph1103I	ATGCAT	1 cut(s)	1011
MroI	TCCGGA	1 cut(s)	1286
MroXI	GAANNNNTTC	1 cut(s)	1818
MscI	TGGCCA	4 cut(s)	53, 945, 1019, 1151
MseI	TTAA	4 cut(s)	438, 675, 1031, 1719
MslI	CAYNNNNRTG	3 cut(s)	501, 1356, 1657
Msp20I	TGGCCA	4 cut(s)	53, 945, 1019, 1151
MspA1I	CMGCKG	1 cut(s)	308
MspI	CCGG	6 cut(s)	42, 97, 112, 1287, 1579, 1648
MspR9I	CCNGG	4 cut(s)	939, 1579, 1580, 1649
Mva1269I	GAATGC	1 cut(s)	497
MvaI	CCWGG	1 cut(s)	939
MwoI	GCNNNNNNNGC	9 cut(s)	407, 646, 658, 720, 974, 1317, 1423, 1448, 1922
NciI	CCSGG	3 cut(s)	1579, 1580, 1649
NcoI	CCATGG	1 cut(s)	773
NdeI	CATATG	2 cut(s)	1117, 1174
NdeII	GATC	8 cut(s)	129, 295, 559, 705, 1458, 1558, 1570, 1700
NlaIV	GGNNCC	8 cut(s)	210, 325, 648, 882, 1534, 1584, 1664, 1827
NmuCI	GTSAC	4 cut(s)	22, 272, 627, 1618
NsbI	TGCGCA	1 cut(s)	258
NsiI	ATGCAT	1 cut(s)	1011
NspI	RCATGY	2 cut(s)	958, 1324
PaeI	GCATGC	1 cut(s)	1324
PagI	TCATGA	1 cut(s)	1851
PceI	AGGCCT	2 cut(s)	1037, 1749
PcsI	WCGNNNNNNNCGW	1 cut(s)	141
PctI	GAATGC	1 cut(s)	497
PdmI	GAANNNNTTC	1 cut(s)	1818
PfeI	GAWTC	4 cut(s)	106, 1283, 1794, 1848
PflMI	CCANNNNNTGG	3 cut(s)	779, 1011, 1082
PkrI	GCNGC	7 cut(s)	70, 511, 651, 685, 1113, 1326, 1453
Ple19I	CGATCG	1 cut(s)	132
PleI	GAGTC	4 cut(s)	145, 292, 641, 742
PmaCI	CACGTG	1 cut(s)	1655
PmlI	CACGTG	1 cut(s)	1655
PpsI	GAGTC	4 cut(s)	145, 292, 641, 742
Ppu21I	YACGTR	1 cut(s)	1655
PpuMI	RGGWCCY	1 cut(s)	1825
Psp5II	RGGWCCY	1 cut(s)	1825
Psp6I	CCWGG	1 cut(s)	937
PspCI	CACGTG	1 cut(s)	1655
PspFI	CCCAGC	1 cut(s)	779
PspGI	CCWGG	1 cut(s)	937
PspN4I	GGNNCC	8 cut(s)	210, 325, 648, 882, 1534, 1584, 1664, 1827
PspPI	GGNCC	4 cut(s)	555, 1532, 1563, 1825
PspPPI	RGGWCCY	1 cut(s)	1825
PsuI	RGATCY	1 cut(s)	1570
PvuI	CGATCG	1 cut(s)	132
PvuII	CAGCTG	1 cut(s)	308
RsaI	GTAC	5 cut(s)	12, 882, 1063, 1316, 1362
RsaNI	GTAC	5 cut(s)	11, 881, 1062, 1315, 1361
RseI	CAYNNNNRTG	3 cut(s)	501, 1356, 1657
SaqAI	TTAA	4 cut(s)	438, 675, 1031, 1719
SatI	GCNGC	7 cut(s)	69, 510, 650, 684, 1112, 1325, 1452
Sau3AI	GATC	8 cut(s)	129, 295, 559, 705, 1458, 1558, 1570, 1700
Sau96I	GGNCC	4 cut(s)	555, 1532, 1563, 1825
SchI	GAGTC	4 cut(s)	146, 293, 642, 742
ScrFI	CCNGG	4 cut(s)	939, 1579, 1580, 1649
SduI	GDGCHC	3 cut(s)	234, 531, 1654
SfaNI	GCATC	3 cut(s)	229, 301, 606
SinI	GGWCC	3 cut(s)	555, 1532, 1825
SmaI	CCCGGG	1 cut(s)	1580
SmiMI	CAYNNNNRTG	3 cut(s)	501, 1356, 1657
SmlI	CTYRAG	1 cut(s)	288
SmoI	CTYRAG	1 cut(s)	288
SphI	GCATGC	1 cut(s)	1324
Sse9I	AATT	4 cut(s)	369, 1371, 1632, 1874
SseBI	AGGCCT	2 cut(s)	1037, 1749
SsiI	CCGC	7 cut(s)	5, 121, 327, 650, 926, 1111, 1318
SspMI	CTAG	3 cut(s)	1259, 1427, 1683
StuI	AGGCCT	2 cut(s)	1037, 1749
StyD4I	CCNGG	4 cut(s)	937, 1577, 1578, 1647
StyI	CCWWGG	1 cut(s)	773
TaaI	ACNGT	5 cut(s)	64, 673, 814, 854, 1165
TaiI	ACGT	5 cut(s)	222, 1294, 1591, 1642, 1657
TaqI	TCGA	1 cut(s)	132
TasI	AATT	4 cut(s)	369, 1371, 1632, 1874
TauI	GCSGC	2 cut(s)	652, 1114
TfiI	GAWTC	4 cut(s)	106, 1283, 1794, 1848
Tru1I	TTAA	4 cut(s)	438, 675, 1031, 1719
Tru9I	TTAA	4 cut(s)	438, 675, 1031, 1719
TscAI	CASTG	4 cut(s)	337, 607, 859, 1152
TseFI	GTSAC	4 cut(s)	22, 272, 627, 1618
TseI	GCWGC	5 cut(s)	68, 509, 683, 1324, 1451
Tsp45I	GTSAC	4 cut(s)	22, 272, 627, 1618
TspGWI	ACGGA	3 cut(s)	744, 1400, 1460
TspMI	CCCGGG	1 cut(s)	1578
TspRI	CASTG	4 cut(s)	337, 607, 859, 1152
Van91I	CCANNNNNTGG	3 cut(s)	779, 1011, 1082
VpaK11BI	GGWCC	3 cut(s)	555, 1532, 1825
XapI	RAATTY	2 cut(s)	369, 1874
XceI	RCATGY	2 cut(s)	958, 1324
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1012, 1543
XmaI	CCCGGG	1 cut(s)	1578
XmnI	GAANNNNTTC	1 cut(s)	1818
XspI	CTAG	3 cut(s)	1259, 1427, 1683
Zsp2I	ATGCAT	1 cut(s)	1011

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.