Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0014317

Physical Location & Seq

Genomic Coordinates Forward (+)

15759266 .. 15763406

Gene Structure 4141 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000007317

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1941 bp

ATGGCGGTAGTACCTCTCTTCGTCACGGCGACGAAGCTCGCCGGATTATTGGCCACCCTCACCGTCGCTGCCAATGTCTTCTCGTTCTCACGCTACCGGAAGAAGAATCTCCGGCCGTTCCGCTCCCCGATCGACGAGTCCTCCGACCCTCTCGCCGACTTCAATCTCAACGAAGGGGAAGATGGGTTCTTCTTTGGGTTGGCGACGGCTCCAGCGCACGTTGAAGACGGGCTAAATGATGCTTGGCTTCAGTTTGCGCAACAAAATCCTTGTGACAAATCAGAGTCTCAAGGTGATCGGCAGACAGCTGATGCTATAATGGGTTCTGCCACTGGCGATGGTGGGTCTCAGCCAGCTCCCCCATCGGGAATTCCTAAGAAAAAGAAGCCTCCTAAGATAGCTATGGAAGCCATGATTAGAGGGTATGCAAAGTATATTAAAGGAGACACAGAAGAAGAAGAAGAAGAAGAAGAAAAAAAACCTGTCCCAAATGAAGAATGCCATCATAATGTAGCTGCATGGCACAATGTTGAGCACCCAGAGGAGAGGATAAAGTTTTGGTCCGATCCTGATAGAGAGCTAAAACTGGCTAAAGATACTGGCATCAGTGTCTTCCGAATGGGAATAGATTGGTCACGAGTCATGCCAGAGGAGCCGCTCAATGGGCTTACAAAAACTGTTAACTATGCTGCATTGGAGCGATACAAGTGGATCATCGGCAGGGTTCGCTCATACGGAATGAAGGTGATGTTGACTCTCTTCCATCACTCATTGCCACCATGGGCTGGGGAGTATGGAGGGTGGAAAGTGGAAAAGACAGTTGATTATTTCTTGGATTTCACAAGGCTTGTTGTTGACAGTGTATCAGATATGATAGACTATTGGGTACCATTCAATGAGCCTCATGTCTTCTGTATGCTTACCTATTGTGCGGGTGCATGGCCTGGTGGCCATCCTGACATGCTGGAGGTTGCGACTTCTGCTCTACCAACTGGTGTTTTCAAACAGGCCATGCATTGGATGGCCATTGCACACTTAAAGGCCTATGACTATATCCACGAGAAAAGTACCTCATCCAAGCCATTAGTTGGAGTGGCACACCATGTCTCATTCATGCGGCCATATGGTCTCTTTGATGTTCCTGCCGTTACACTGGCCAACTCCATGACTGTCTTCCCATATGCGGATAGCATTTCTGACAAGCTGGATTTTATTGGCATAAACTACTATGGACAGGAAGTGGTGTGTGGTGCTGGACTGAAGCTAGTAGAGACTGACGAATATAGTGAATCCGGACGTGGGATATACCCAGATGGCTTGTATCGCATGCTGCTTCAGTTCAATGACAGATATAAACATCTAAATGTACCCTTCATAATTACTGAAAATGGGGTATCTGATGAGACAGATGTAATCCGTCGGCCTTACCTGCTAGAGCATTTGCTTGCTGTTCATGCAGCCAAGATCATGGGTGTCCGTGTGCTTGGTTACCTGTTTTGGACTATTTCTGATAACTGGGAATGGGCAGATGGGTATGGTCCCAAGTTTGGACTGGTAGCAGTTGATCGGGCCAAAGATCTTGCCCGGGTTCCACGTCCTTCATACCATCTATTTACTAAGGTGGTGACAACAGGTAAAATTACACGTTATGACCGGGCGCGTGCATGGCACCAACTCCAAATAGCTGCTAGGGAAAAGAAAATGCGATCCTTTTATCGGGCTGTTAATAAAGATGGTTTGATGTATGCAGGAGGCCTTGATGAACCTATACAGAGACCCTATGTTGAAAGAGATTGGCGATTCGGACACTATGAGATGGAAGGTCTTCAGGACCCCATTTGTCGCTTATGGAGATTCATGACCCGACCTGCCACCCTAAAATTTAGGAAAAGGAAACCCCAGATGGATGAAGAGGATGAAGCTCTATTGGCTCTCCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0008150 GO:0008152 GO:0008378 GO:0008422 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009628 GO:0009707 GO:0009941 GO:0015926 GO:0016020 GO:0016021 GO:0016740 GO:0016757 GO:0016758 GO:0016787 GO:0016798 GO:0019867 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031350 GO:0031351 GO:0031354 GO:0031355 GO:0031358 GO:0031359 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050826 GO:0050896 GO:0071704 GO:0080079 GO:0098588 GO:0098805 GO:1901135 GO:1901657

KEGG Pathways

Metabolic & Signaling

ko00561 map00561

Pfam Domains

Protein Families

Glyco_hydro_1

Protein Analysis

647

Amino Acids

73.52

Weight (kDa)

6.16

Isoelectric Point (pI)

38.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_1	PF00232	184 - 376	3.5e-29	Glycosyl hydrolase family 1
Glyco_hydro_1	PF00232	397 - 544	2.8e-20	Glycosyl hydrolase family 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0014317)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06510 AT3G06510
fragaria_vesca	FvH4_4g20710
malus_domestica	MD16G1148600.v1.1
prunus_persica	Prupe.1G112100_v2.0.a1 Prupe.1G112100_v2.0.a1 Prupe.1G112100_v2.0.a1
pyrus_communis	pycom16g12690
rosa_chinensis	RchiOBHm_Chr4g0426131
rosa_laevigata	RLG00000007317
rosa_multiflora	Rmu_co8494203.1_g000001
rosa_roxburghii	Rroxscaffold_5G00368120
rosa_rugosa	Rorug04G0209300
rosa_samantha	Rh4AG265800 Rh4CG284800 Rh4DG268000
rosa_wichuraiana	Rw4G022970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	258
Acc36I	ACCTGC	2 cut(s)	1437, 1876
Acc65I	GGTACC	1 cut(s)	886
AccB1I	GGYRCC	2 cut(s)	886, 1668
AccB7I	CCANNNNNTGG	3 cut(s)	785, 1017, 1088
AccBSI	CCGCTC	2 cut(s)	123, 658
AccII	CGCG	1 cut(s)	1660
AccIII	TCCGGA	1 cut(s)	1292
AciI	CCGC	6 cut(s)	5, 121, 656, 932, 1117, 1184
AclWI	GGATC	3 cut(s)	560, 719, 1701
AcoI	YGGCCR	6 cut(s)	51, 113, 949, 1023, 1118, 1155
AcsI	RAATTY	2 cut(s)	369, 1880
AcuI	CTGAAG	4 cut(s)	233, 1280, 1319, 1811
AfaI	GTAC	4 cut(s)	12, 888, 1069, 1368
AfiI	CCNNNNNNNGG	9 cut(s)	365, 662, 785, 1017, 1088, 1183, 1299, 1547, 1716
AflIII	ACRYGT	1 cut(s)	1643
AgsI	TTSAA	6 cut(s)	163, 224, 895, 1003, 1342, 1787
AjiI	CACGTC	2 cut(s)	1298, 1595
AjnI	CCWGG	1 cut(s)	943
AloI	GAACNNNNNNTCC	2 cut(s)	170, 202
Alw21I	GWGCWC	1 cut(s)	537
Alw26I	GTCTC	8 cut(s)	291, 351, 438, 1111, 1133, 1265, 1397, 1768
AlwI	GGATC	3 cut(s)	560, 719, 1701
Ama87I	CYCGRG	1 cut(s)	1584
Aor13HI	TCCGGA	1 cut(s)	1292
ApeKI	GCWGC	6 cut(s)	68, 515, 689, 1330, 1457, 1685
ApoI	RAATTY	2 cut(s)	369, 1880
Asp700I	GAANNNNTTC	1 cut(s)	1824
Asp718I	GGTACC	1 cut(s)	886
AspLEI	GCGC	3 cut(s)	217, 259, 1660
AspS9I	GGNCC	4 cut(s)	561, 1538, 1569, 1831
AsuC2I	CCSGG	3 cut(s)	1585, 1586, 1655
AsuHPI	GGTGA	4 cut(s)	52, 305, 757, 1636
AvaI	CYCGRG	1 cut(s)	1584
AvaII	GGWCC	3 cut(s)	561, 1538, 1831
BalI	TGGCCA	4 cut(s)	53, 951, 1025, 1157
BanI	GGYRCC	2 cut(s)	886, 1668
BauI	CACGAG	2 cut(s)	636, 1058
BbsI	GAAGAC	6 cut(s)	70, 231, 604, 901, 1165, 1817
Bbv12I	GWGCWC	1 cut(s)	537
BbvI	GCAGC	6 cut(s)	55, 502, 676, 1317, 1469, 1672
BceAI	ACGGC	4 cut(s)	42, 100, 222, 1130
BciT130I	CCWGG	1 cut(s)	945
BcnI	CCSGG	3 cut(s)	1585, 1586, 1655
BcoDI	GTCTC	8 cut(s)	291, 351, 438, 1111, 1133, 1265, 1397, 1768
BfaI	CTAG	3 cut(s)	1265, 1433, 1689
BfuAI	ACCTGC	2 cut(s)	1437, 1876
BglII	AGATCT	1 cut(s)	1576
BisI	GCNGC	8 cut(s)	69, 516, 656, 690, 1118, 1331, 1458, 1686
BlsI	GCNGC	8 cut(s)	70, 517, 657, 691, 1119, 1332, 1459, 1687
Bme1390I	CCNGG	4 cut(s)	945, 1585, 1586, 1655
Bme18I	GGWCC	3 cut(s)	561, 1538, 1831
BmeT110I	CYCGRG	1 cut(s)	1584
BmgBI	CACGTC	2 cut(s)	1298, 1595
BmgT120I	GGNCC	4 cut(s)	561, 1538, 1569, 1831
BmiI	GGNNCC	7 cut(s)	210, 654, 888, 1540, 1590, 1670, 1833
BmrFI	CCNGG	4 cut(s)	945, 1585, 1586, 1655
BmrI	ACTGGG	1 cut(s)	1525
BmsI	GCATC	3 cut(s)	229, 301, 612
BmuI	ACTGGG	1 cut(s)	1525
BpiI	GAAGAC	6 cut(s)	70, 231, 604, 901, 1165, 1817
BpmI	CTGGAG	3 cut(s)	195, 986, 1919
BpuEI	CTTGAG	1 cut(s)	273
BpuMI	CCSGG	3 cut(s)	1585, 1586, 1655
BsaI	GGTCTC	3 cut(s)	351, 1133, 1768
BsaJI	CCNNGG	2 cut(s)	779, 1584
BsaWI	WCCGGW	2 cut(s)	96, 1292
BsaXI	ACNNNNNCTCC	2 cut(s)	125, 155
Bsc4I	CCNNNNNNNGG	9 cut(s)	365, 662, 785, 1017, 1088, 1183, 1299, 1547, 1716
Bse1I	ACTGG	8 cut(s)	337, 591, 604, 997, 1158, 1520, 1557, 1936
Bse3DI	GCAATG	2 cut(s)	770, 1026
BseAI	TCCGGA	1 cut(s)	1292
BseBI	CCWGG	1 cut(s)	945
BseDI	CCNNGG	2 cut(s)	779, 1584
BseGI	GGATG	5 cut(s)	952, 1026, 1073, 1912, 1921
BseLI	CCNNNNNNNGG	9 cut(s)	365, 662, 785, 1017, 1088, 1183, 1299, 1547, 1716
BseMI	GCAATG	2 cut(s)	770, 1026
BseMII	CTCAG	1 cut(s)	362
BseNI	ACTGG	8 cut(s)	337, 591, 604, 997, 1158, 1520, 1557, 1936
BseRI	GAGGAG	2 cut(s)	557, 665
BseX3I	CGGCCG	1 cut(s)	113
BseXI	GCAGC	6 cut(s)	55, 502, 676, 1317, 1469, 1672
BseYI	CCCAGC	1 cut(s)	785
Bsh1236I	CGCG	1 cut(s)	1660
Bsh1285I	CGRYCG	2 cut(s)	116, 132
BshNI	GGYRCC	2 cut(s)	886, 1668
BsiEI	CGRYCG	2 cut(s)	116, 132
BsiHKAI	GWGCWC	1 cut(s)	537
BsiHKCI	CYCGRG	1 cut(s)	1584
BsiSI	CCGG	6 cut(s)	42, 97, 112, 1293, 1585, 1654
BslFI	GGGAC	2 cut(s)	470, 1524
BslI	CCNNNNNNNGG	9 cut(s)	365, 662, 785, 1017, 1088, 1183, 1299, 1547, 1716
BsmAI	GTCTC	8 cut(s)	291, 351, 438, 1111, 1133, 1265, 1397, 1768
BsmFI	GGGAC	2 cut(s)	470, 1524
BsmI	GAATGC	1 cut(s)	503
Bso31I	GGTCTC	3 cut(s)	351, 1133, 1768
BsoBI	CYCGRG	1 cut(s)	1584
Bsp1286I	GDGCHC	1 cut(s)	537
Bsp13I	TCCGGA	1 cut(s)	1292
Bsp143I	GATC	8 cut(s)	129, 295, 565, 711, 1464, 1564, 1576, 1706
Bsp19I	CCATGG	1 cut(s)	779
BspACI	CCGC	6 cut(s)	5, 121, 656, 932, 1117, 1184
BspCNI	CTCAG	1 cut(s)	361
BspEI	TCCGGA	1 cut(s)	1292
BspFNI	CGCG	1 cut(s)	1660
BspHI	TCATGA	1 cut(s)	1857
BspLI	GGNNCC	7 cut(s)	210, 654, 888, 1540, 1590, 1670, 1833
BspMI	ACCTGC	2 cut(s)	1437, 1876
BspPI	GGATC	3 cut(s)	560, 719, 1701
BspT107I	GGYRCC	2 cut(s)	886, 1668
BspTNI	GGTCTC	3 cut(s)	351, 1133, 1768
BsrBI	CCGCTC	2 cut(s)	123, 658
BsrDI	GCAATG	2 cut(s)	770, 1026
BsrI	ACTGG	8 cut(s)	337, 591, 604, 997, 1158, 1520, 1557, 1936
BssECI	CCNNGG	2 cut(s)	779, 1584
BssMI	GATC	8 cut(s)	129, 295, 565, 711, 1464, 1564, 1576, 1706
BssSI	CACGAG	2 cut(s)	636, 1058
BssT1I	CCWWGG	1 cut(s)	779
Bst2BI	CACGAG	2 cut(s)	636, 1058
Bst2UI	CCWGG	1 cut(s)	945
Bst4CI	ACNGT	5 cut(s)	64, 679, 820, 860, 1171
Bst6I	CTCTTC	3 cut(s)	23, 764, 1905
BstC8I	GCNNGC	5 cut(s)	39, 354, 1328, 1446, 1662
BstDEI	CTNAG	4 cut(s)	348, 375, 393, 1617
BstDSI	CCRYGG	1 cut(s)	779
BstEII	GGTNACC	1 cut(s)	1487
BstF5I	GGATG	5 cut(s)	952, 1026, 1073, 1912, 1921
BstFNI	CGCG	1 cut(s)	1660
BstHHI	GCGC	3 cut(s)	217, 259, 1660
BstKTI	GATC	8 cut(s)	132, 298, 568, 714, 1467, 1567, 1579, 1709
BstMAI	GTCTC	8 cut(s)	291, 351, 438, 1111, 1133, 1265, 1397, 1768
BstMBI	GATC	8 cut(s)	129, 295, 565, 711, 1464, 1564, 1576, 1706
BstMCI	CGRYCG	2 cut(s)	116, 132
BstNI	CCWGG	1 cut(s)	945
BstNSI	RCATGY	2 cut(s)	964, 1330
BstPI	GGTNACC	1 cut(s)	1487
BstSCI	CCNGG	4 cut(s)	943, 1583, 1584, 1653
BstUI	CGCG	1 cut(s)	1660
BstV1I	GCAGC	6 cut(s)	55, 502, 676, 1317, 1469, 1672
BstV2I	GAAGAC	6 cut(s)	70, 231, 604, 901, 1165, 1817
BstX2I	RGATCY	1 cut(s)	1576
BstXI	CCANNNNNNTGG	1 cut(s)	1468
BstYI	RGATCY	1 cut(s)	1576
BstZI	CGGCCG	1 cut(s)	113
BtgI	CCRYGG	1 cut(s)	779
BtgZI	GCGATG	1 cut(s)	351
BtrI	CACGTC	2 cut(s)	1298, 1595
BtsCI	GGATG	5 cut(s)	952, 1026, 1073, 1912, 1921
BtsIMutI	CAGTG	4 cut(s)	330, 613, 865, 1151
BveI	ACCTGC	2 cut(s)	1437, 1876
Cac8I	GCNNGC	5 cut(s)	39, 354, 1328, 1446, 1662
CciI	TCATGA	1 cut(s)	1857
CfoI	GCGC	3 cut(s)	217, 259, 1660
Cfr13I	GGNCC	4 cut(s)	561, 1538, 1569, 1831
Cfr9I	CCCGGG	1 cut(s)	1584
Csp6I	GTAC	4 cut(s)	11, 887, 1068, 1367
CviQI	GTAC	4 cut(s)	11, 887, 1068, 1367
DdeI	CTNAG	4 cut(s)	348, 375, 393, 1617
DpnI	GATC	8 cut(s)	131, 297, 567, 713, 1466, 1566, 1578, 1708
DpnII	GATC	8 cut(s)	129, 295, 565, 711, 1464, 1564, 1576, 1706
EaeI	YGGCCR	6 cut(s)	51, 113, 949, 1023, 1118, 1155
EagI	CGGCCG	1 cut(s)	113
Eam1104I	CTCTTC	3 cut(s)	23, 764, 1905
EarI	CTCTTC	3 cut(s)	23, 764, 1905
EclXI	CGGCCG	1 cut(s)	113
Eco130I	CCWWGG	1 cut(s)	779
Eco147I	AGGCCT	2 cut(s)	1043, 1755
Eco31I	GGTCTC	3 cut(s)	351, 1133, 1768
Eco47I	GGWCC	3 cut(s)	561, 1538, 1831
Eco52I	CGGCCG	1 cut(s)	113
Eco57I	CTGAAG	4 cut(s)	233, 1280, 1319, 1811
Eco88I	CYCGRG	1 cut(s)	1584
Eco91I	GGTNACC	1 cut(s)	1487
EcoO109I	RGGNCCY	1 cut(s)	1831
EcoO65I	GGTNACC	1 cut(s)	1487
EcoRI	GAATTC	1 cut(s)	369
EcoRII	CCWGG	1 cut(s)	943
EcoT14I	CCWWGG	1 cut(s)	779
EcoT22I	ATGCAT	1 cut(s)	1017
ErhI	CCWWGG	1 cut(s)	779
FaqI	GGGAC	2 cut(s)	470, 1524
FauI	CCCGC	1 cut(s)	925
FauNDI	CATATG	2 cut(s)	1123, 1180
Fnu4HI	GCNGC	8 cut(s)	69, 516, 656, 690, 1118, 1331, 1458, 1686
FokI	GGATG	5 cut(s)	939, 1033, 1060, 1919, 1928
Fsp4HI	GCNGC	8 cut(s)	69, 516, 656, 690, 1118, 1331, 1458, 1686
FspBI	CTAG	3 cut(s)	1265, 1433, 1689
FspI	TGCGCA	1 cut(s)	258
GlaI	GCGC	3 cut(s)	216, 258, 1659
GluI	GCNGC	8 cut(s)	69, 516, 656, 690, 1118, 1331, 1458, 1686
GsaI	CCCAGC	1 cut(s)	789
GsuI	CTGGAG	3 cut(s)	195, 986, 1919
HapII	CCGG	6 cut(s)	42, 97, 112, 1293, 1585, 1654
HhaI	GCGC	3 cut(s)	217, 259, 1660
Hin6I	GCGC	3 cut(s)	215, 257, 1658
HinP1I	GCGC	3 cut(s)	215, 257, 1658
HincII	GTYRAC	3 cut(s)	682, 753, 856
HindII	GTYRAC	3 cut(s)	682, 753, 856
HinfI	GANTC	8 cut(s)	106, 137, 284, 639, 754, 1289, 1800, 1854
HpaI	GTTAAC	1 cut(s)	682
HpaII	CCGG	6 cut(s)	42, 97, 112, 1293, 1585, 1654
HphI	GGTGA	4 cut(s)	52, 305, 757, 1636
Hpy166II	GTNNAC	3 cut(s)	682, 753, 856
Hpy188I	TCNGA	9 cut(s)	145, 283, 565, 617, 868, 1198, 1399, 1510, 1805
Hpy188III	TCNNGA	7 cut(s)	366, 569, 636, 956, 1293, 1829, 1858
Hpy8I	GTNNAC	3 cut(s)	682, 753, 856
Hpy99I	CGWCG	5 cut(s)	34, 68, 137, 208, 1422
HpyAV	CCTTC	5 cut(s)	167, 736, 1381, 1608, 1814
HpyCH4III	ACNGT	5 cut(s)	64, 679, 820, 860, 1171
HpyCH4IV	ACGT	4 cut(s)	219, 1297, 1594, 1645
HpyCH4V	TGCA	9 cut(s)	428, 518, 692, 938, 1015, 1031, 1457, 1664, 1748
HpyF3I	CTNAG	4 cut(s)	348, 375, 393, 1617
HpySE526I	ACGT	4 cut(s)	219, 1297, 1594, 1645
HspAI	GCGC	3 cut(s)	215, 257, 1658
Kpn2I	TCCGGA	1 cut(s)	1292
KpnI	GGTACC	1 cut(s)	890
KspAI	GTTAAC	1 cut(s)	682
Kzo9I	GATC	8 cut(s)	129, 295, 565, 711, 1464, 1564, 1576, 1706
LmnI	GCTCC	5 cut(s)	128, 214, 361, 652, 697
Lsp1109I	GCAGC	6 cut(s)	55, 502, 676, 1317, 1469, 1672
LweI	GCATC	3 cut(s)	229, 301, 612
MaeI	CTAG	3 cut(s)	1265, 1433, 1689
MaeII	ACGT	4 cut(s)	219, 1297, 1594, 1645
MaeIII	GTNAC	6 cut(s)	22, 272, 633, 1147, 1487, 1624
MalI	GATC	8 cut(s)	131, 297, 567, 713, 1466, 1566, 1578, 1708
MbiI	CCGCTC	2 cut(s)	123, 658
MboI	GATC	8 cut(s)	129, 295, 565, 711, 1464, 1564, 1576, 1706
MflI	RGATCY	1 cut(s)	1576
MhlI	GDGCHC	1 cut(s)	537
MlsI	TGGCCA	4 cut(s)	53, 951, 1025, 1157
MluCI	AATT	4 cut(s)	369, 1377, 1638, 1880
MluNI	TGGCCA	4 cut(s)	53, 951, 1025, 1157
MlyI	GAGTC	4 cut(s)	146, 293, 648, 748
MmeI	TCCRAC	2 cut(s)	168, 1069
Mox20I	TGGCCA	4 cut(s)	53, 951, 1025, 1157
Mph1103I	ATGCAT	1 cut(s)	1017
MroI	TCCGGA	1 cut(s)	1292
MroXI	GAANNNNTTC	1 cut(s)	1824
MscI	TGGCCA	4 cut(s)	53, 951, 1025, 1157
MseI	TTAA	4 cut(s)	438, 681, 1037, 1725
MslI	CAYNNNNRTG	2 cut(s)	507, 1362
Msp20I	TGGCCA	4 cut(s)	53, 951, 1025, 1157
MspA1I	CMGCKG	1 cut(s)	308
MspI	CCGG	6 cut(s)	42, 97, 112, 1293, 1585, 1654
MspR9I	CCNGG	4 cut(s)	945, 1585, 1586, 1655
Mva1269I	GAATGC	1 cut(s)	503
MvaI	CCWGG	1 cut(s)	945
MvnI	CGCG	1 cut(s)	1660
NciI	CCSGG	3 cut(s)	1585, 1586, 1655
NcoI	CCATGG	1 cut(s)	779
NdeI	CATATG	2 cut(s)	1123, 1180
NdeII	GATC	8 cut(s)	129, 295, 565, 711, 1464, 1564, 1576, 1706
NlaIV	GGNNCC	7 cut(s)	210, 654, 888, 1540, 1590, 1670, 1833
NmuCI	GTSAC	4 cut(s)	22, 272, 633, 1624
NsbI	TGCGCA	1 cut(s)	258
NsiI	ATGCAT	1 cut(s)	1017
NspI	RCATGY	2 cut(s)	964, 1330
PaeI	GCATGC	1 cut(s)	1330
PagI	TCATGA	1 cut(s)	1857
PceI	AGGCCT	2 cut(s)	1043, 1755
PcsI	WCGNNNNNNNCGW	1 cut(s)	141
PctI	GAATGC	1 cut(s)	503
PdmI	GAANNNNTTC	1 cut(s)	1824
PfeI	GAWTC	4 cut(s)	106, 1289, 1800, 1854
PflMI	CCANNNNNTGG	3 cut(s)	785, 1017, 1088
PkrI	GCNGC	8 cut(s)	70, 517, 657, 691, 1119, 1332, 1459, 1687
Ple19I	CGATCG	1 cut(s)	132
PleI	GAGTC	4 cut(s)	145, 292, 647, 748
PpsI	GAGTC	4 cut(s)	145, 292, 647, 748
PpuMI	RGGWCCY	1 cut(s)	1831
Psp5II	RGGWCCY	1 cut(s)	1831
Psp6I	CCWGG	1 cut(s)	943
PspEI	GGTNACC	1 cut(s)	1487
PspFI	CCCAGC	1 cut(s)	785
PspGI	CCWGG	1 cut(s)	943
PspN4I	GGNNCC	7 cut(s)	210, 654, 888, 1540, 1590, 1670, 1833
PspPI	GGNCC	4 cut(s)	561, 1538, 1569, 1831
PspPPI	RGGWCCY	1 cut(s)	1831
PsuI	RGATCY	1 cut(s)	1576
PvuI	CGATCG	1 cut(s)	132
PvuII	CAGCTG	1 cut(s)	308
RsaI	GTAC	4 cut(s)	12, 888, 1069, 1368
RsaNI	GTAC	4 cut(s)	11, 887, 1068, 1367
RseI	CAYNNNNRTG	2 cut(s)	507, 1362
SaqAI	TTAA	4 cut(s)	438, 681, 1037, 1725
SatI	GCNGC	8 cut(s)	69, 516, 656, 690, 1118, 1331, 1458, 1686
Sau3AI	GATC	8 cut(s)	129, 295, 565, 711, 1464, 1564, 1576, 1706
Sau96I	GGNCC	4 cut(s)	561, 1538, 1569, 1831
SchI	GAGTC	4 cut(s)	146, 293, 648, 748
ScrFI	CCNGG	4 cut(s)	945, 1585, 1586, 1655
SduI	GDGCHC	1 cut(s)	537
SfaNI	GCATC	3 cut(s)	229, 301, 612
SinI	GGWCC	3 cut(s)	561, 1538, 1831
SmaI	CCCGGG	1 cut(s)	1586
SmiMI	CAYNNNNRTG	2 cut(s)	507, 1362
SmlI	CTYRAG	1 cut(s)	288
SmoI	CTYRAG	1 cut(s)	288
SphI	GCATGC	1 cut(s)	1330
Sse9I	AATT	4 cut(s)	369, 1377, 1638, 1880
SseBI	AGGCCT	2 cut(s)	1043, 1755
SsiI	CCGC	6 cut(s)	5, 121, 656, 932, 1117, 1184
SspMI	CTAG	3 cut(s)	1265, 1433, 1689
StuI	AGGCCT	2 cut(s)	1043, 1755
StyD4I	CCNGG	4 cut(s)	943, 1583, 1584, 1653
StyI	CCWWGG	1 cut(s)	779
TaaI	ACNGT	5 cut(s)	64, 679, 820, 860, 1171
TaiI	ACGT	4 cut(s)	222, 1300, 1597, 1648
TaqI	TCGA	1 cut(s)	132
TasI	AATT	4 cut(s)	369, 1377, 1638, 1880
TauI	GCSGC	2 cut(s)	658, 1120
TfiI	GAWTC	4 cut(s)	106, 1289, 1800, 1854
Tru1I	TTAA	4 cut(s)	438, 681, 1037, 1725
Tru9I	TTAA	4 cut(s)	438, 681, 1037, 1725
TscAI	CASTG	4 cut(s)	337, 613, 865, 1158
TseFI	GTSAC	4 cut(s)	22, 272, 633, 1624
TseI	GCWGC	6 cut(s)	68, 515, 689, 1330, 1457, 1685
Tsp45I	GTSAC	4 cut(s)	22, 272, 633, 1624
TspGWI	ACGGA	3 cut(s)	750, 1406, 1466
TspMI	CCCGGG	1 cut(s)	1584
TspRI	CASTG	4 cut(s)	337, 613, 865, 1158
Van91I	CCANNNNNTGG	3 cut(s)	785, 1017, 1088
VpaK11BI	GGWCC	3 cut(s)	561, 1538, 1831
XapI	RAATTY	2 cut(s)	369, 1880
XceI	RCATGY	2 cut(s)	964, 1330
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1018, 1549
XmaI	CCCGGG	1 cut(s)	1584
XmnI	GAANNNNTTC	1 cut(s)	1824
XspI	CTAG	3 cut(s)	1265, 1433, 1689
Zsp2I	ATGCAT	1 cut(s)	1017

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.