Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0014317

Physical Location & Seq

Genomic Coordinates Reverse (-)

48743818 .. 48748401

Gene Structure 4584 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG268000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1683 bp

ATGGCGGTAGTACCTCTCTTCGTCACGGCAACGAAGCTCGCCGGATTATTGGCCACCCTCACCGTCGCTGCCAATGTCTTCTCTTTCTCACGCTACCGGAAGAAGAATCTCCGGCCGTTCCGCTCCCCGATCGACGAGTCCTCCGACCCTCTCGCCGACTTCAATCTCAACGAAGGGGAAGATGGGTTCTTCTTTGGGTTGGCGACGGCTCCAGCGCACGTTGAAGACGGGCTCAATGATGCTTGGCTTCAGTTTGCGCAAGAAAATCCTTGTGACAAATCAGAGTCTCAAGGTGATCGGCAGACAGCTGATGCTATAATGGGTTCCGCCACTGGCGATGGTGGGTCTCAGACAGCTCCCCCATTGGGAATTCCTAAGAAAAAGAAGCCTCCTAAGATAGCTATGGAAGCCATGATTAGAGGGTATGCAAAGTATATTAAAGGGGACACAGGAGAAGAAGAAGAAGAAAAAAAACCTGTCCCAAATGAAGAATGCCATCATAATGTAGCTGCATGGCACAATGTTGAGCACCCAGAGGAGAGGATAAAGTTTTGGTCCGATCCTGATACAGAGCTAAAACTGGCTAAAGATACTGGCATCAGTGTCTTCCGAATGGGAATAGATTGGTCACGAGTCATGCCTGAGGAGCCGCTCAATGGGCTTACAAAAACTGTTAACTATGCTGCATTGGAGCGATACAAGTGGATCATCGGCAGGGTTCGCTCATACGGAATGAAGGTGATGTTGACTCTCTTCCATCACTCATTGCCACCATGGGCTGGGGAGTATGGAGGGTGGAAAATGGAAAAGACAGTTGATTATTTCTTGGATTTCACAAGGCTTGTTGTTGACAGTGTATCAGATATGATAGACTATTGGGTACCATTCAATGAGCCTCATGTCTTCTGTATGCTTACCTATTGTGCGGGTGCATGGCCTGGTGGCCATCCTGACATGCTGGAGGTTGCGACTTCTGCTCTACCAACTGGTGTTTTCAAACAGGCCATGCATTGGATGGCCATTGCACACTTAAAGGCCTATGACTATATCCACGAAAAAAGTACCTCATCCAAGCCATTAGTTGGAGTGGCACACCATGTCTCATTCATGCGGCCATATGGTCTCTTTGATGTTCCTGCTGTTACACTGGCCAACTCCATGACTGTCTTCCCATATGTGGATAGTATTTCTGACAAGCTGGATTTTATTGGCATAAACTACTATGGACAGGAAGTGGTGTGTGGTGCTGGACTGAAGCTAGTAGAGACTGATGAATATAGTGAATCCGGACGTGGGATATACCCAGATGGCTTGTACCGCATGCTGCTTCAGTTCAATGACAGATATAAACATCTAAATGTACCCTTCATAATTACTGAAAATGGGGTATCTGATGAGACAGATGTAATCCGTCGGCCTTACCTGCTAGAGCATTTACTCGCTGTTCATGCAGCCAAGATCATGGGTGTCCGTGTGCTTGGTTTCCTGTTTTGGACTATTTCTGATAACTGGGAGTGGGCAGATGGGTATGGTCCCAAGTTTGGACTGGTAGCAGTTGATCGGGCCAAAGATCTTGCCCGGGTTCCACGTCCTTCATACCATCTATTTACTAAGGTTAACGAGTTTCTTCACTGCTCAGATACTTCTTTCTGCTCCGCTACCCACCCCCAAAATGAGCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0008150 GO:0008152 GO:0008378 GO:0008422 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009628 GO:0009707 GO:0009941 GO:0015926 GO:0016020 GO:0016021 GO:0016740 GO:0016757 GO:0016758 GO:0016787 GO:0016798 GO:0019867 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031350 GO:0031351 GO:0031354 GO:0031355 GO:0031358 GO:0031359 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050826 GO:0050896 GO:0071704 GO:0080079 GO:0098588 GO:0098805 GO:1901135 GO:1901657

KEGG Pathways

Metabolic & Signaling

ko00561 map00561

Pfam Domains

Protein Families

Glyco_hydro_1

Protein Analysis

560

Amino Acids

62.93

Weight (kDa)

5.63

Isoelectric Point (pI)

31.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_1	PF00232	182 - 374	1.5e-29	Glycosyl hydrolase family 1
Glyco_hydro_1	PF00232	395 - 541	6.6e-19	Glycosyl hydrolase family 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014317)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06510 AT3G06510
fragaria_vesca	FvH4_4g20710
malus_domestica	MD16G1148600.v1.1
prunus_persica	Prupe.1G112100_v2.0.a1 Prupe.1G112100_v2.0.a1 Prupe.1G112100_v2.0.a1
pyrus_communis	pycom16g12690
rosa_chinensis	RchiOBHm_Chr4g0426131
rosa_laevigata	RLG00000007317
rosa_multiflora	Rmu_co8494203.1_g000001
rosa_roxburghii	Rroxscaffold_5G00368120
rosa_rugosa	Rorug04G0209300
rosa_samantha	Rh4AG265800 Rh4CG284800 Rh4DG268000
rosa_wichuraiana	Rw4G022970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	258
Acc36I	ACCTGC	1 cut(s)	1431
Acc65I	GGTACC	1 cut(s)	880
AccB1I	GGYRCC	1 cut(s)	880
AccB7I	CCANNNNNTGG	3 cut(s)	779, 1011, 1082
AccBSI	CCGCTC	2 cut(s)	123, 652
AccIII	TCCGGA	1 cut(s)	1286
AciI	CCGC	8 cut(s)	5, 121, 327, 650, 926, 1111, 1318, 1656
AclWI	GGATC	2 cut(s)	554, 713
AcoI	YGGCCR	6 cut(s)	51, 113, 943, 1017, 1112, 1149
AcsI	RAATTY	1 cut(s)	369
AcuI	CTGAAG	3 cut(s)	233, 1274, 1313
AfaI	GTAC	5 cut(s)	12, 882, 1063, 1316, 1362
AfiI	CCNNNNNNNGG	8 cut(s)	365, 656, 779, 1011, 1082, 1177, 1293, 1541
AgsI	TTSAA	5 cut(s)	163, 224, 889, 997, 1336
AjiI	CACGTC	2 cut(s)	1292, 1589
AjnI	CCWGG	1 cut(s)	937
AloI	GAACNNNNNNTCC	2 cut(s)	170, 202
AluBI	AGCT	8 cut(s)	37, 308, 356, 401, 509, 574, 1198, 1258
AluI	AGCT	8 cut(s)	37, 308, 356, 401, 509, 574, 1198, 1258
Alw21I	GWGCWC	1 cut(s)	531
Alw26I	GTCTC	6 cut(s)	291, 351, 1105, 1127, 1259, 1391
AlwI	GGATC	2 cut(s)	554, 713
Ama87I	CYCGRG	1 cut(s)	1578
Aor13HI	TCCGGA	1 cut(s)	1286
ApeKI	GCWGC	5 cut(s)	68, 509, 683, 1324, 1451
ApoI	RAATTY	1 cut(s)	369
Asp718I	GGTACC	1 cut(s)	880
AspLEI	GCGC	2 cut(s)	217, 259
AspS9I	GGNCC	3 cut(s)	555, 1532, 1563
AsuC2I	CCSGG	2 cut(s)	1579, 1580
AsuHPI	GGTGA	3 cut(s)	52, 305, 751
AvaI	CYCGRG	1 cut(s)	1578
AvaII	GGWCC	2 cut(s)	555, 1532
AxyI	CCTNAGG	1 cut(s)	642
BalI	TGGCCA	4 cut(s)	53, 945, 1019, 1151
BanI	GGYRCC	1 cut(s)	880
BanII	GRGCYC	1 cut(s)	234
BauI	CACGAG	1 cut(s)	630
BbsI	GAAGAC	5 cut(s)	70, 231, 598, 895, 1159
Bbv12I	GWGCWC	1 cut(s)	531
BbvI	GCAGC	5 cut(s)	55, 496, 670, 1311, 1463
BccI	CCATC	9 cut(s)	176, 332, 504, 765, 954, 1009, 1301, 1517, 1608
BceAI	ACGGC	3 cut(s)	42, 100, 222
BciT130I	CCWGG	1 cut(s)	939
BcnI	CCSGG	2 cut(s)	1579, 1580
BcoDI	GTCTC	6 cut(s)	291, 351, 1105, 1127, 1259, 1391
BfaI	CTAG	2 cut(s)	1259, 1427
BfuAI	ACCTGC	1 cut(s)	1431
BglII	AGATCT	1 cut(s)	1570
BisI	GCNGC	7 cut(s)	69, 510, 650, 684, 1112, 1325, 1452
BlsI	GCNGC	7 cut(s)	70, 511, 651, 685, 1113, 1326, 1453
Bme1390I	CCNGG	3 cut(s)	939, 1579, 1580
Bme18I	GGWCC	2 cut(s)	555, 1532
BmeT110I	CYCGRG	1 cut(s)	1578
BmgBI	CACGTC	2 cut(s)	1292, 1589
BmgT120I	GGNCC	3 cut(s)	555, 1532, 1563
BmiI	GGNNCC	6 cut(s)	210, 325, 648, 882, 1534, 1584
BmrFI	CCNGG	3 cut(s)	939, 1579, 1580
BmrI	ACTGGG	1 cut(s)	1519
BmsI	GCATC	3 cut(s)	229, 301, 606
BmuI	ACTGGG	1 cut(s)	1519
BpiI	GAAGAC	5 cut(s)	70, 231, 598, 895, 1159
BpmI	CTGGAG	2 cut(s)	195, 980
BpuEI	CTTGAG	1 cut(s)	273
BpuMI	CCSGG	2 cut(s)	1579, 1580
BsaI	GGTCTC	2 cut(s)	351, 1127
BsaJI	CCNNGG	2 cut(s)	773, 1578
BsaWI	WCCGGW	2 cut(s)	96, 1286
BsaXI	ACNNNNNCTCC	2 cut(s)	125, 155
Bsc4I	CCNNNNNNNGG	8 cut(s)	365, 656, 779, 1011, 1082, 1177, 1293, 1541
Bse1I	ACTGG	7 cut(s)	337, 585, 598, 991, 1152, 1514, 1551
Bse21I	CCTNAGG	1 cut(s)	642
Bse3DI	GCAATG	2 cut(s)	764, 1020
BseAI	TCCGGA	1 cut(s)	1286
BseBI	CCWGG	1 cut(s)	939
BseDI	CCNNGG	2 cut(s)	773, 1578
BseGI	GGATG	3 cut(s)	946, 1020, 1067
BseLI	CCNNNNNNNGG	8 cut(s)	365, 656, 779, 1011, 1082, 1177, 1293, 1541
BseMI	GCAATG	2 cut(s)	764, 1020
BseMII	CTCAG	3 cut(s)	362, 633, 1650
BseNI	ACTGG	7 cut(s)	337, 585, 598, 991, 1152, 1514, 1551
BseRI	GAGGAG	2 cut(s)	551, 659
BseX3I	CGGCCG	1 cut(s)	113
BseXI	GCAGC	5 cut(s)	55, 496, 670, 1311, 1463
BseYI	CCCAGC	1 cut(s)	779
Bsh1285I	CGRYCG	2 cut(s)	116, 132
BshNI	GGYRCC	1 cut(s)	880
BsiEI	CGRYCG	2 cut(s)	116, 132
BsiHKAI	GWGCWC	1 cut(s)	531
BsiHKCI	CYCGRG	1 cut(s)	1578
BsiSI	CCGG	5 cut(s)	42, 97, 112, 1287, 1579
BslFI	GGGAC	3 cut(s)	458, 464, 1518
BslI	CCNNNNNNNGG	8 cut(s)	365, 656, 779, 1011, 1082, 1177, 1293, 1541
BsmAI	GTCTC	6 cut(s)	291, 351, 1105, 1127, 1259, 1391
BsmFI	GGGAC	3 cut(s)	458, 464, 1518
BsmI	GAATGC	1 cut(s)	497
Bso31I	GGTCTC	2 cut(s)	351, 1127
BsoBI	CYCGRG	1 cut(s)	1578
Bsp1286I	GDGCHC	2 cut(s)	234, 531
Bsp13I	TCCGGA	1 cut(s)	1286
Bsp143I	GATC	7 cut(s)	129, 295, 559, 705, 1458, 1558, 1570
Bsp19I	CCATGG	1 cut(s)	773
BspACI	CCGC	8 cut(s)	5, 121, 327, 650, 926, 1111, 1318, 1656
BspCNI	CTCAG	3 cut(s)	361, 634, 1649
BspEI	TCCGGA	1 cut(s)	1286
BspLI	GGNNCC	6 cut(s)	210, 325, 648, 882, 1534, 1584
BspMI	ACCTGC	1 cut(s)	1431
BspPI	GGATC	2 cut(s)	554, 713
BspT107I	GGYRCC	1 cut(s)	880
BspTNI	GGTCTC	2 cut(s)	351, 1127
BsrBI	CCGCTC	2 cut(s)	123, 652
BsrDI	GCAATG	2 cut(s)	764, 1020
BsrI	ACTGG	7 cut(s)	337, 585, 598, 991, 1152, 1514, 1551
BssECI	CCNNGG	2 cut(s)	773, 1578
BssMI	GATC	7 cut(s)	129, 295, 559, 705, 1458, 1558, 1570
BssSI	CACGAG	1 cut(s)	630
BssT1I	CCWWGG	1 cut(s)	773
Bst2BI	CACGAG	1 cut(s)	630
Bst2UI	CCWGG	1 cut(s)	939
Bst4CI	ACNGT	5 cut(s)	64, 673, 814, 854, 1165
Bst6I	CTCTTC	2 cut(s)	23, 758
BstC8I	GCNNGC	2 cut(s)	39, 1322
BstDEI	CTNAG	6 cut(s)	348, 375, 393, 642, 1611, 1636
BstDSI	CCRYGG	1 cut(s)	773
BstF5I	GGATG	3 cut(s)	946, 1020, 1067
BstHHI	GCGC	2 cut(s)	217, 259
BstKTI	GATC	7 cut(s)	132, 298, 562, 708, 1461, 1561, 1573
BstMAI	GTCTC	6 cut(s)	291, 351, 1105, 1127, 1259, 1391
BstMBI	GATC	7 cut(s)	129, 295, 559, 705, 1458, 1558, 1570
BstMCI	CGRYCG	2 cut(s)	116, 132
BstMWI	GCNNNNNNNGC	8 cut(s)	407, 646, 658, 720, 974, 1317, 1423, 1448
BstNI	CCWGG	1 cut(s)	939
BstNSI	RCATGY	2 cut(s)	958, 1324
BstSCI	CCNGG	3 cut(s)	937, 1577, 1578
BstV1I	GCAGC	5 cut(s)	55, 496, 670, 1311, 1463
BstV2I	GAAGAC	5 cut(s)	70, 231, 598, 895, 1159
BstX2I	RGATCY	1 cut(s)	1570
BstXI	CCANNNNNNTGG	1 cut(s)	1462
BstYI	RGATCY	1 cut(s)	1570
BstZI	CGGCCG	1 cut(s)	113
Bsu36I	CCTNAGG	1 cut(s)	642
BtgI	CCRYGG	1 cut(s)	773
BtgZI	GCGATG	1 cut(s)	351
BtrI	CACGTC	2 cut(s)	1292, 1589
BtsCI	GGATG	3 cut(s)	946, 1020, 1067
BtsI	GCAGTG	1 cut(s)	1630
BtsIMutI	CAGTG	5 cut(s)	330, 607, 859, 1145, 1630
BveI	ACCTGC	1 cut(s)	1431
Cac8I	GCNNGC	2 cut(s)	39, 1322
CfoI	GCGC	2 cut(s)	217, 259
Cfr13I	GGNCC	3 cut(s)	555, 1532, 1563
Cfr9I	CCCGGG	1 cut(s)	1578
Csp6I	GTAC	5 cut(s)	11, 881, 1062, 1315, 1361
CviQI	GTAC	5 cut(s)	11, 881, 1062, 1315, 1361
DdeI	CTNAG	6 cut(s)	348, 375, 393, 642, 1611, 1636
DpnI	GATC	7 cut(s)	131, 297, 561, 707, 1460, 1560, 1572
DpnII	GATC	7 cut(s)	129, 295, 559, 705, 1458, 1558, 1570
EaeI	YGGCCR	6 cut(s)	51, 113, 943, 1017, 1112, 1149
EagI	CGGCCG	1 cut(s)	113
Eam1104I	CTCTTC	2 cut(s)	23, 758
EarI	CTCTTC	2 cut(s)	23, 758
EciI	GGCGGA	1 cut(s)	316
EclXI	CGGCCG	1 cut(s)	113
Eco130I	CCWWGG	1 cut(s)	773
Eco147I	AGGCCT	1 cut(s)	1037
Eco24I	GRGCYC	1 cut(s)	234
Eco31I	GGTCTC	2 cut(s)	351, 1127
Eco47I	GGWCC	2 cut(s)	555, 1532
Eco52I	CGGCCG	1 cut(s)	113
Eco57I	CTGAAG	3 cut(s)	233, 1274, 1313
Eco81I	CCTNAGG	1 cut(s)	642
Eco88I	CYCGRG	1 cut(s)	1578
EcoRI	GAATTC	1 cut(s)	369
EcoRII	CCWGG	1 cut(s)	937
EcoT14I	CCWWGG	1 cut(s)	773
EcoT22I	ATGCAT	1 cut(s)	1011
EcoT38I	GRGCYC	1 cut(s)	234
ErhI	CCWWGG	1 cut(s)	773
FaqI	GGGAC	3 cut(s)	458, 464, 1518
FauI	CCCGC	1 cut(s)	919
FauNDI	CATATG	2 cut(s)	1117, 1174
Fnu4HI	GCNGC	7 cut(s)	69, 510, 650, 684, 1112, 1325, 1452
FokI	GGATG	3 cut(s)	933, 1027, 1054
FriOI	GRGCYC	1 cut(s)	234
Fsp4HI	GCNGC	7 cut(s)	69, 510, 650, 684, 1112, 1325, 1452
FspBI	CTAG	2 cut(s)	1259, 1427
FspI	TGCGCA	1 cut(s)	258
GlaI	GCGC	2 cut(s)	216, 258
GluI	GCNGC	7 cut(s)	69, 510, 650, 684, 1112, 1325, 1452
GsaI	CCCAGC	1 cut(s)	783
GsuI	CTGGAG	2 cut(s)	195, 980
HapII	CCGG	5 cut(s)	42, 97, 112, 1287, 1579
HhaI	GCGC	2 cut(s)	217, 259
Hin6I	GCGC	2 cut(s)	215, 257
HinP1I	GCGC	2 cut(s)	215, 257
HincII	GTYRAC	4 cut(s)	676, 747, 850, 1618
HindII	GTYRAC	4 cut(s)	676, 747, 850, 1618
HinfI	GANTC	6 cut(s)	106, 137, 284, 633, 748, 1283
HpaI	GTTAAC	2 cut(s)	676, 1618
HpaII	CCGG	5 cut(s)	42, 97, 112, 1287, 1579
HphI	GGTGA	3 cut(s)	52, 305, 751
Hpy166II	GTNNAC	4 cut(s)	676, 747, 850, 1618
Hpy188III	TCNNGA	4 cut(s)	563, 630, 950, 1287
Hpy8I	GTNNAC	4 cut(s)	676, 747, 850, 1618
Hpy99I	CGWCG	4 cut(s)	68, 137, 208, 1416
HpyAV	CCTTC	4 cut(s)	167, 730, 1375, 1602
HpyCH4III	ACNGT	5 cut(s)	64, 673, 814, 854, 1165
HpyCH4IV	ACGT	3 cut(s)	219, 1291, 1588
HpyCH4V	TGCA	7 cut(s)	428, 512, 686, 932, 1009, 1025, 1451
HpyF10VI	GCNNNNNNNGC	8 cut(s)	407, 646, 658, 720, 974, 1317, 1423, 1448
HpyF3I	CTNAG	6 cut(s)	348, 375, 393, 642, 1611, 1636
HpySE526I	ACGT	3 cut(s)	219, 1291, 1588
HspAI	GCGC	2 cut(s)	215, 257
Kpn2I	TCCGGA	1 cut(s)	1286
KpnI	GGTACC	1 cut(s)	884
KspAI	GTTAAC	2 cut(s)	676, 1618
Kzo9I	GATC	7 cut(s)	129, 295, 559, 705, 1458, 1558, 1570
LmnI	GCTCC	6 cut(s)	128, 214, 361, 646, 691, 1658
Lsp1109I	GCAGC	5 cut(s)	55, 496, 670, 1311, 1463
LweI	GCATC	3 cut(s)	229, 301, 606
MaeI	CTAG	2 cut(s)	1259, 1427
MaeII	ACGT	3 cut(s)	219, 1291, 1588
MaeIII	GTNAC	4 cut(s)	22, 272, 627, 1141
MalI	GATC	7 cut(s)	131, 297, 561, 707, 1460, 1560, 1572
MbiI	CCGCTC	2 cut(s)	123, 652
MboI	GATC	7 cut(s)	129, 295, 559, 705, 1458, 1558, 1570
MflI	RGATCY	1 cut(s)	1570
MhlI	GDGCHC	2 cut(s)	234, 531
MlsI	TGGCCA	4 cut(s)	53, 945, 1019, 1151
MluCI	AATT	2 cut(s)	369, 1371
MluNI	TGGCCA	4 cut(s)	53, 945, 1019, 1151
MlyI	GAGTC	4 cut(s)	146, 293, 642, 742
MmeI	TCCRAC	2 cut(s)	168, 1063
Mox20I	TGGCCA	4 cut(s)	53, 945, 1019, 1151
Mph1103I	ATGCAT	1 cut(s)	1011
MroI	TCCGGA	1 cut(s)	1286
MscI	TGGCCA	4 cut(s)	53, 945, 1019, 1151
MseI	TTAA	5 cut(s)	438, 675, 1031, 1617, 1681
MslI	CAYNNNNRTG	2 cut(s)	501, 1356
Msp20I	TGGCCA	4 cut(s)	53, 945, 1019, 1151
MspA1I	CMGCKG	1 cut(s)	308
MspI	CCGG	5 cut(s)	42, 97, 112, 1287, 1579
MspR9I	CCNGG	3 cut(s)	939, 1579, 1580
Mva1269I	GAATGC	1 cut(s)	497
MvaI	CCWGG	1 cut(s)	939
MwoI	GCNNNNNNNGC	8 cut(s)	407, 646, 658, 720, 974, 1317, 1423, 1448
NciI	CCSGG	2 cut(s)	1579, 1580
NcoI	CCATGG	1 cut(s)	773
NdeI	CATATG	2 cut(s)	1117, 1174
NdeII	GATC	7 cut(s)	129, 295, 559, 705, 1458, 1558, 1570
NlaIV	GGNNCC	6 cut(s)	210, 325, 648, 882, 1534, 1584
NmuCI	GTSAC	3 cut(s)	22, 272, 627
NsbI	TGCGCA	1 cut(s)	258
NsiI	ATGCAT	1 cut(s)	1011
NspI	RCATGY	2 cut(s)	958, 1324
PaeI	GCATGC	1 cut(s)	1324
PceI	AGGCCT	1 cut(s)	1037
PcsI	WCGNNNNNNNCGW	1 cut(s)	141
PctI	GAATGC	1 cut(s)	497
PfeI	GAWTC	2 cut(s)	106, 1283
PflMI	CCANNNNNTGG	3 cut(s)	779, 1011, 1082
PkrI	GCNGC	7 cut(s)	70, 511, 651, 685, 1113, 1326, 1453
Ple19I	CGATCG	1 cut(s)	132
PleI	GAGTC	4 cut(s)	145, 292, 641, 742
PpsI	GAGTC	4 cut(s)	145, 292, 641, 742
Psp6I	CCWGG	1 cut(s)	937
PspFI	CCCAGC	1 cut(s)	779
PspGI	CCWGG	1 cut(s)	937
PspN4I	GGNNCC	6 cut(s)	210, 325, 648, 882, 1534, 1584
PspPI	GGNCC	3 cut(s)	555, 1532, 1563
PsuI	RGATCY	1 cut(s)	1570
PvuI	CGATCG	1 cut(s)	132
PvuII	CAGCTG	1 cut(s)	308
RsaI	GTAC	5 cut(s)	12, 882, 1063, 1316, 1362
RsaNI	GTAC	5 cut(s)	11, 881, 1062, 1315, 1361
RseI	CAYNNNNRTG	2 cut(s)	501, 1356
SaqAI	TTAA	5 cut(s)	438, 675, 1031, 1617, 1681
SatI	GCNGC	7 cut(s)	69, 510, 650, 684, 1112, 1325, 1452
Sau3AI	GATC	7 cut(s)	129, 295, 559, 705, 1458, 1558, 1570
Sau96I	GGNCC	3 cut(s)	555, 1532, 1563
SchI	GAGTC	4 cut(s)	146, 293, 642, 742
ScrFI	CCNGG	3 cut(s)	939, 1579, 1580
SduI	GDGCHC	2 cut(s)	234, 531
SfaNI	GCATC	3 cut(s)	229, 301, 606
SinI	GGWCC	2 cut(s)	555, 1532
SmaI	CCCGGG	1 cut(s)	1580
SmiMI	CAYNNNNRTG	2 cut(s)	501, 1356
SmlI	CTYRAG	1 cut(s)	288
SmoI	CTYRAG	1 cut(s)	288
SphI	GCATGC	1 cut(s)	1324
Sse9I	AATT	2 cut(s)	369, 1371
SseBI	AGGCCT	1 cut(s)	1037
SsiI	CCGC	8 cut(s)	5, 121, 327, 650, 926, 1111, 1318, 1656
SspMI	CTAG	2 cut(s)	1259, 1427
StuI	AGGCCT	1 cut(s)	1037
StyD4I	CCNGG	3 cut(s)	937, 1577, 1578
StyI	CCWWGG	1 cut(s)	773
TaaI	ACNGT	5 cut(s)	64, 673, 814, 854, 1165
TaiI	ACGT	3 cut(s)	222, 1294, 1591
TaqI	TCGA	1 cut(s)	132
TasI	AATT	2 cut(s)	369, 1371
TauI	GCSGC	2 cut(s)	652, 1114
TfiI	GAWTC	2 cut(s)	106, 1283
Tru1I	TTAA	5 cut(s)	438, 675, 1031, 1617, 1681
Tru9I	TTAA	5 cut(s)	438, 675, 1031, 1617, 1681
TscAI	CASTG	5 cut(s)	337, 607, 859, 1152, 1637
TseFI	GTSAC	3 cut(s)	22, 272, 627
TseI	GCWGC	5 cut(s)	68, 509, 683, 1324, 1451
Tsp45I	GTSAC	3 cut(s)	22, 272, 627
TspDTI	ATGAA	7 cut(s)	501, 749, 1096, 1287, 1357, 1436, 1584
TspGWI	ACGGA	3 cut(s)	744, 1400, 1460
TspMI	CCCGGG	1 cut(s)	1578
TspRI	CASTG	5 cut(s)	337, 607, 859, 1152, 1637
Van91I	CCANNNNNTGG	3 cut(s)	779, 1011, 1082
VpaK11BI	GGWCC	2 cut(s)	555, 1532
XapI	RAATTY	1 cut(s)	369
XceI	RCATGY	2 cut(s)	958, 1324
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1012, 1543
XmaI	CCCGGG	1 cut(s)	1578
XspI	CTAG	2 cut(s)	1259, 1427
Zsp2I	ATGCAT	1 cut(s)	1011

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.