Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0014317

Physical Location & Seq

Genomic Coordinates Reverse (-)

51614657 .. 51619277

Gene Structure 4621 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ39521

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1932 bp

ATGGCGGTAGTACCTCTCTTCGTCACGGCAACGAAGCTCGCCGGATTATTGGCCACCCTGACCGTCGCTGCTAATGTCTTCTCTTTCTCACGCTACCGGAAGAAGAATCTCCGGCCGTTCCGCTCCCCGATCGACGAGTCCTCCGACCCTCTCGCCGACTTCAATCTCAACGAAGGGGAAGATGGGTTCTTCTTTGGGTTGGCGACGGCTCCAGCGCACGTTGAAGACGGGCTAAATGATGCTTGGCTTCAGTTTGCGCAAGAAAATCCTTGTGACAAATCAGAGTCTCAAGGTGATCGGCAGACAGCTGATGCTATAATGGGTTCCGCCACTGGCGATGGTGGGTCTCAGCCAGCTCCCCCATTGGGAATTCCTAAGAAAAAGAAGCCTCCTAAGATAGCTATGGAAGCCATGATTAGAGGGTATGCAAAGTATATTAAAGGGGACACAGGAGAAGAAGAAGAAGAAAAACCTGTCCCAAATGAAGAATGCCATCATAATGTAGCTGCATGGCACAATGTTGAGCACCCAGAGGAGAGGATAAAGTTTTGGTCCGATCCTGATACAGAGCTAAAACTGGCTAAAGATACTGGTATCAGTGTCTTCCGAATGGGAATAGATTGGTCACGAGTCATGCCAGAGGAGCCGCTCAATGGGCTTACAAAAACTGTTAACTATGCTGCATTGGAGCGATACAAGTGGATCATCGGCAGGGTTCACTCATACGGAATGAAGGTGATGTTGACTCTCTTCCATCACTCATTGCCACCATGGGCTGGGGAGTATGGAGGGTGGAAAGTGGAAAAGACAGTTGATTATTTCTTGGATTTCACAAGGCTTGTTGTTGACAGTGTATCAGATATGATAGACTATTGGGTACCATTCAATGAGCCTCATGTCTTCTGTATGCTTACCTATTGTGCGGGTGCATGGCCTGGTGGCCATCCTGACATGCTGGAGGTTGCGACCTCTGCTCTACCAACTGGTGTTTTCAAACAGGCCATGCATTGGATGGCCATTGCACACTTAAAGGCCTATGACTATATCCACGAAAAAAGTACCTCATCCAAGCCATTAGTTGGAGTGGCACACCATGTCTCATTCATGCGGCCATATGGTCTCTTTGATGTTCCTGCTGTTACACTGGCCAACTCCATGACTGTCTTCCCATATGTGGATAGTATTTCTGACAAGCTGGATTTTATTGGCATAAACTACTATGGACAGGAAGTGGTGTGTGGTGCTGGACTGAAGCTAGTAGAGACTGATGAATATAGTGAATCCGGACGTGGGATATACCCAGATGGCTTGTACCGCATGCTGCTTCAGTTCAATGACAGATATAAACATCTAAATGTACCCTTCATAATTACTGAAAATGGGGTATCTGATGAGACAGATGTAATCCGTCGGCCTTACCTGCTAGAGCATTTGCTCGCTGTTCATGCAGCCAAGATCATGGGTGTCCGTGTGCTTGGTTTCCTGTTTTGGACTATTTCTGATAACTGGGAGTGGGCAGATGGGTATGGTCCCAAGTTTGGACTGGTAGCAGTTGATCGGGCCAAAGATCTTGCCCGGGTTCCACGTCCTTCATACCATCTATTTACTAAGGTGGTGACAACAGGTAAAATTACACGTTATGACCGGGCACGTGCATGGCACCAACTCCAAATATCTGCTAGAGAAAAGAAAATGCGATCCTTTTATCGGGCTGTTAATAAAGATGGTTTGATGTATGCAGGAGGCCTTGATGAACCTATACGGAGACCCTATGTTGAAAGAGATTGGCGATTCGGACACTATGAGATGGAAGGTCTTCAGGACCCCATTTGTCGCTTGTGGAGATTCATGACCCGACCTGCCACCCTAAAATTTAGGAAAAGGAAACCCCAGATGGATGAAGAGGATGAAGCTCTATTGGCTCTCCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0008150 GO:0008152 GO:0008378 GO:0008422 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009628 GO:0009707 GO:0009941 GO:0015926 GO:0016020 GO:0016021 GO:0016740 GO:0016757 GO:0016758 GO:0016787 GO:0016798 GO:0019867 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031350 GO:0031351 GO:0031354 GO:0031355 GO:0031358 GO:0031359 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050826 GO:0050896 GO:0071704 GO:0080079 GO:0098588 GO:0098805 GO:1901135 GO:1901657

KEGG Pathways

Metabolic & Signaling

ko00561 map00561

Pfam Domains

Protein Families

Glyco_hydro_1

Protein Analysis

643

Amino Acids

73.07

Weight (kDa)

6.18

Isoelectric Point (pI)

36.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_1	PF00232	183 - 373	1.8e-29	Glycosyl hydrolase family 1
Glyco_hydro_1	PF00232	394 - 541	2.6e-19	Glycosyl hydrolase family 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014317)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06510 AT3G06510
fragaria_vesca	FvH4_4g20710
malus_domestica	MD16G1148600.v1.1
prunus_persica	Prupe.1G112100_v2.0.a1 Prupe.1G112100_v2.0.a1 Prupe.1G112100_v2.0.a1
pyrus_communis	pycom16g12690
rosa_chinensis	RchiOBHm_Chr4g0426131
rosa_laevigata	RLG00000007317
rosa_multiflora	Rmu_co8494203.1_g000001
rosa_roxburghii	Rroxscaffold_5G00368120
rosa_rugosa	Rorug04G0209300
rosa_samantha	Rh4AG265800 Rh4CG284800 Rh4DG268000
rosa_wichuraiana	Rw4G022970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	258
Acc36I	ACCTGC	2 cut(s)	1428, 1867
Acc65I	GGTACC	1 cut(s)	877
AccB1I	GGYRCC	2 cut(s)	877, 1659
AccB7I	CCANNNNNTGG	3 cut(s)	776, 1008, 1079
AccBSI	CCGCTC	2 cut(s)	123, 649
AccIII	TCCGGA	1 cut(s)	1283
AciI	CCGC	7 cut(s)	5, 121, 327, 647, 923, 1108, 1315
AclWI	GGATC	3 cut(s)	551, 710, 1692
AcoI	YGGCCR	6 cut(s)	51, 113, 940, 1014, 1109, 1146
AcsI	RAATTY	2 cut(s)	369, 1871
AcuI	CTGAAG	4 cut(s)	233, 1271, 1310, 1802
AcvI	CACGTG	1 cut(s)	1652
AfaI	GTAC	5 cut(s)	12, 879, 1060, 1313, 1359
AfiI	CCNNNNNNNGG	9 cut(s)	365, 653, 776, 1008, 1079, 1174, 1290, 1538, 1707
AflIII	ACRYGT	1 cut(s)	1634
AgsI	TTSAA	6 cut(s)	163, 224, 886, 994, 1333, 1778
AjiI	CACGTC	2 cut(s)	1289, 1586
AjnI	CCWGG	1 cut(s)	934
AloI	GAACNNNNNNTCC	2 cut(s)	170, 202
AluBI	AGCT	9 cut(s)	37, 308, 356, 401, 506, 571, 1195, 1255, 1913
AluI	AGCT	9 cut(s)	37, 308, 356, 401, 506, 571, 1195, 1255, 1913
Alw21I	GWGCWC	1 cut(s)	528
Alw26I	GTCTC	7 cut(s)	291, 351, 1102, 1124, 1256, 1388, 1759
AlwI	GGATC	3 cut(s)	551, 710, 1692
Ama87I	CYCGRG	1 cut(s)	1575
Aor13HI	TCCGGA	1 cut(s)	1283
ApeKI	GCWGC	5 cut(s)	68, 506, 680, 1321, 1448
ApoI	RAATTY	2 cut(s)	369, 1871
Asp700I	GAANNNNTTC	1 cut(s)	1815
Asp718I	GGTACC	1 cut(s)	877
AspLEI	GCGC	2 cut(s)	217, 259
AspS9I	GGNCC	4 cut(s)	552, 1529, 1560, 1822
AsuC2I	CCSGG	3 cut(s)	1576, 1577, 1646
AsuHPI	GGTGA	3 cut(s)	305, 748, 1627
AvaI	CYCGRG	1 cut(s)	1575
AvaII	GGWCC	3 cut(s)	552, 1529, 1822
BaeGI	GKGCMC	1 cut(s)	1651
BalI	TGGCCA	4 cut(s)	53, 942, 1016, 1148
BanI	GGYRCC	2 cut(s)	877, 1659
BauI	CACGAG	1 cut(s)	627
BbrPI	CACGTG	1 cut(s)	1652
BbsI	GAAGAC	6 cut(s)	70, 231, 595, 892, 1156, 1808
Bbv12I	GWGCWC	1 cut(s)	528
BbvI	GCAGC	5 cut(s)	55, 493, 667, 1308, 1460
BceAI	ACGGC	3 cut(s)	42, 100, 222
BciT130I	CCWGG	1 cut(s)	936
BcnI	CCSGG	3 cut(s)	1576, 1577, 1646
BcoDI	GTCTC	7 cut(s)	291, 351, 1102, 1124, 1256, 1388, 1759
BfaI	CTAG	3 cut(s)	1256, 1424, 1680
BfuAI	ACCTGC	2 cut(s)	1428, 1867
BglII	AGATCT	1 cut(s)	1567
BisI	GCNGC	7 cut(s)	69, 507, 647, 681, 1109, 1322, 1449
BlsI	GCNGC	7 cut(s)	70, 508, 648, 682, 1110, 1323, 1450
Bme1390I	CCNGG	4 cut(s)	936, 1576, 1577, 1646
Bme18I	GGWCC	3 cut(s)	552, 1529, 1822
BmeT110I	CYCGRG	1 cut(s)	1575
BmgBI	CACGTC	2 cut(s)	1289, 1586
BmgT120I	GGNCC	4 cut(s)	552, 1529, 1560, 1822
BmiI	GGNNCC	8 cut(s)	210, 325, 645, 879, 1531, 1581, 1661, 1824
BmrFI	CCNGG	4 cut(s)	936, 1576, 1577, 1646
BmrI	ACTGGG	1 cut(s)	1516
BmsI	GCATC	2 cut(s)	229, 301
BmuI	ACTGGG	1 cut(s)	1516
BpiI	GAAGAC	6 cut(s)	70, 231, 595, 892, 1156, 1808
BpmI	CTGGAG	3 cut(s)	195, 977, 1910
BpuEI	CTTGAG	1 cut(s)	273
BpuMI	CCSGG	3 cut(s)	1576, 1577, 1646
BsaAI	YACGTR	1 cut(s)	1652
BsaI	GGTCTC	3 cut(s)	351, 1124, 1759
BsaJI	CCNNGG	2 cut(s)	770, 1575
BsaWI	WCCGGW	2 cut(s)	96, 1283
BsaXI	ACNNNNNCTCC	2 cut(s)	125, 155
Bsc4I	CCNNNNNNNGG	9 cut(s)	365, 653, 776, 1008, 1079, 1174, 1290, 1538, 1707
Bse1I	ACTGG	8 cut(s)	337, 582, 595, 988, 1149, 1511, 1548, 1927
Bse3DI	GCAATG	2 cut(s)	761, 1017
BseAI	TCCGGA	1 cut(s)	1283
BseBI	CCWGG	1 cut(s)	936
BseDI	CCNNGG	2 cut(s)	770, 1575
BseGI	GGATG	5 cut(s)	943, 1017, 1064, 1903, 1912
BseLI	CCNNNNNNNGG	9 cut(s)	365, 653, 776, 1008, 1079, 1174, 1290, 1538, 1707
BseMI	GCAATG	2 cut(s)	761, 1017
BseMII	CTCAG	1 cut(s)	362
BseNI	ACTGG	8 cut(s)	337, 582, 595, 988, 1149, 1511, 1548, 1927
BseRI	GAGGAG	2 cut(s)	548, 656
BseSI	GKGCMC	1 cut(s)	1651
BseX3I	CGGCCG	1 cut(s)	113
BseXI	GCAGC	5 cut(s)	55, 493, 667, 1308, 1460
BseYI	CCCAGC	1 cut(s)	776
Bsh1285I	CGRYCG	2 cut(s)	116, 132
BshNI	GGYRCC	2 cut(s)	877, 1659
BsiEI	CGRYCG	2 cut(s)	116, 132
BsiHKAI	GWGCWC	1 cut(s)	528
BsiHKCI	CYCGRG	1 cut(s)	1575
BsiSI	CCGG	6 cut(s)	42, 97, 112, 1284, 1576, 1645
BslFI	GGGAC	3 cut(s)	458, 461, 1515
BslI	CCNNNNNNNGG	9 cut(s)	365, 653, 776, 1008, 1079, 1174, 1290, 1538, 1707
BsmAI	GTCTC	7 cut(s)	291, 351, 1102, 1124, 1256, 1388, 1759
BsmFI	GGGAC	3 cut(s)	458, 461, 1515
BsmI	GAATGC	1 cut(s)	494
Bso31I	GGTCTC	3 cut(s)	351, 1124, 1759
BsoBI	CYCGRG	1 cut(s)	1575
Bsp1286I	GDGCHC	2 cut(s)	528, 1651
Bsp13I	TCCGGA	1 cut(s)	1283
Bsp143I	GATC	8 cut(s)	129, 295, 556, 702, 1455, 1555, 1567, 1697
Bsp19I	CCATGG	1 cut(s)	770
BspACI	CCGC	7 cut(s)	5, 121, 327, 647, 923, 1108, 1315
BspCNI	CTCAG	1 cut(s)	361
BspEI	TCCGGA	1 cut(s)	1283
BspHI	TCATGA	1 cut(s)	1848
BspLI	GGNNCC	8 cut(s)	210, 325, 645, 879, 1531, 1581, 1661, 1824
BspMI	ACCTGC	2 cut(s)	1428, 1867
BspPI	GGATC	3 cut(s)	551, 710, 1692
BspT107I	GGYRCC	2 cut(s)	877, 1659
BspTNI	GGTCTC	3 cut(s)	351, 1124, 1759
BsrBI	CCGCTC	2 cut(s)	123, 649
BsrDI	GCAATG	2 cut(s)	761, 1017
BsrI	ACTGG	8 cut(s)	337, 582, 595, 988, 1149, 1511, 1548, 1927
BssECI	CCNNGG	2 cut(s)	770, 1575
BssMI	GATC	8 cut(s)	129, 295, 556, 702, 1455, 1555, 1567, 1697
BssSI	CACGAG	1 cut(s)	627
BssT1I	CCWWGG	1 cut(s)	770
Bst2BI	CACGAG	1 cut(s)	627
Bst2UI	CCWGG	1 cut(s)	936
Bst4CI	ACNGT	5 cut(s)	64, 670, 811, 851, 1162
Bst6I	CTCTTC	3 cut(s)	23, 755, 1896
BstBAI	YACGTR	1 cut(s)	1652
BstC8I	GCNNGC	4 cut(s)	39, 354, 1319, 1437
BstDEI	CTNAG	4 cut(s)	348, 375, 393, 1608
BstDSI	CCRYGG	1 cut(s)	770
BstF5I	GGATG	5 cut(s)	943, 1017, 1064, 1903, 1912
BstHHI	GCGC	2 cut(s)	217, 259
BstKTI	GATC	8 cut(s)	132, 298, 559, 705, 1458, 1558, 1570, 1700
BstMAI	GTCTC	7 cut(s)	291, 351, 1102, 1124, 1256, 1388, 1759
BstMBI	GATC	8 cut(s)	129, 295, 556, 702, 1455, 1555, 1567, 1697
BstMCI	CGRYCG	2 cut(s)	116, 132
BstMWI	GCNNNNNNNGC	8 cut(s)	407, 643, 655, 971, 1314, 1420, 1445, 1919
BstNI	CCWGG	1 cut(s)	936
BstNSI	RCATGY	2 cut(s)	955, 1321
BstSCI	CCNGG	4 cut(s)	934, 1574, 1575, 1644
BstSLI	GKGCMC	1 cut(s)	1651
BstV1I	GCAGC	5 cut(s)	55, 493, 667, 1308, 1460
BstV2I	GAAGAC	6 cut(s)	70, 231, 595, 892, 1156, 1808
BstX2I	RGATCY	1 cut(s)	1567
BstXI	CCANNNNNNTGG	1 cut(s)	1459
BstYI	RGATCY	1 cut(s)	1567
BstZI	CGGCCG	1 cut(s)	113
BtgI	CCRYGG	1 cut(s)	770
BtgZI	GCGATG	1 cut(s)	351
BtrI	CACGTC	2 cut(s)	1289, 1586
BtsCI	GGATG	5 cut(s)	943, 1017, 1064, 1903, 1912
BtsIMutI	CAGTG	4 cut(s)	330, 604, 856, 1142
BveI	ACCTGC	2 cut(s)	1428, 1867
Cac8I	GCNNGC	4 cut(s)	39, 354, 1319, 1437
CciI	TCATGA	1 cut(s)	1848
CfoI	GCGC	2 cut(s)	217, 259
Cfr13I	GGNCC	4 cut(s)	552, 1529, 1560, 1822
Cfr9I	CCCGGG	1 cut(s)	1575
Csp6I	GTAC	5 cut(s)	11, 878, 1059, 1312, 1358
CviQI	GTAC	5 cut(s)	11, 878, 1059, 1312, 1358
DdeI	CTNAG	4 cut(s)	348, 375, 393, 1608
DpnI	GATC	8 cut(s)	131, 297, 558, 704, 1457, 1557, 1569, 1699
DpnII	GATC	8 cut(s)	129, 295, 556, 702, 1455, 1555, 1567, 1697
EaeI	YGGCCR	6 cut(s)	51, 113, 940, 1014, 1109, 1146
EagI	CGGCCG	1 cut(s)	113
Eam1104I	CTCTTC	3 cut(s)	23, 755, 1896
EarI	CTCTTC	3 cut(s)	23, 755, 1896
EciI	GGCGGA	1 cut(s)	316
EclXI	CGGCCG	1 cut(s)	113
Eco130I	CCWWGG	1 cut(s)	770
Eco147I	AGGCCT	2 cut(s)	1034, 1746
Eco31I	GGTCTC	3 cut(s)	351, 1124, 1759
Eco47I	GGWCC	3 cut(s)	552, 1529, 1822
Eco52I	CGGCCG	1 cut(s)	113
Eco57I	CTGAAG	4 cut(s)	233, 1271, 1310, 1802
Eco72I	CACGTG	1 cut(s)	1652
Eco88I	CYCGRG	1 cut(s)	1575
EcoO109I	RGGNCCY	1 cut(s)	1822
EcoRI	GAATTC	1 cut(s)	369
EcoRII	CCWGG	1 cut(s)	934
EcoT14I	CCWWGG	1 cut(s)	770
EcoT22I	ATGCAT	1 cut(s)	1008
ErhI	CCWWGG	1 cut(s)	770
FaqI	GGGAC	3 cut(s)	458, 461, 1515
FauI	CCCGC	1 cut(s)	916
FauNDI	CATATG	2 cut(s)	1114, 1171
Fnu4HI	GCNGC	7 cut(s)	69, 507, 647, 681, 1109, 1322, 1449
FokI	GGATG	5 cut(s)	930, 1024, 1051, 1910, 1919
Fsp4HI	GCNGC	7 cut(s)	69, 507, 647, 681, 1109, 1322, 1449
FspBI	CTAG	3 cut(s)	1256, 1424, 1680
FspI	TGCGCA	1 cut(s)	258
GlaI	GCGC	2 cut(s)	216, 258
GluI	GCNGC	7 cut(s)	69, 507, 647, 681, 1109, 1322, 1449
GsaI	CCCAGC	1 cut(s)	780
GsuI	CTGGAG	3 cut(s)	195, 977, 1910
HapII	CCGG	6 cut(s)	42, 97, 112, 1284, 1576, 1645
HhaI	GCGC	2 cut(s)	217, 259
Hin6I	GCGC	2 cut(s)	215, 257
HinP1I	GCGC	2 cut(s)	215, 257
HincII	GTYRAC	3 cut(s)	673, 744, 847
HindII	GTYRAC	3 cut(s)	673, 744, 847
HinfI	GANTC	8 cut(s)	106, 137, 284, 630, 745, 1280, 1791, 1845
HpaI	GTTAAC	1 cut(s)	673
HpaII	CCGG	6 cut(s)	42, 97, 112, 1284, 1576, 1645
HphI	GGTGA	3 cut(s)	305, 748, 1627
Hpy166II	GTNNAC	4 cut(s)	673, 718, 744, 847
Hpy188I	TCNGA	9 cut(s)	145, 283, 556, 608, 859, 1189, 1390, 1501, 1796
Hpy188III	TCNNGA	6 cut(s)	560, 627, 947, 1284, 1820, 1849
Hpy8I	GTNNAC	4 cut(s)	673, 718, 744, 847
Hpy99I	CGWCG	4 cut(s)	68, 137, 208, 1413
HpyAV	CCTTC	5 cut(s)	167, 727, 1372, 1599, 1805
HpyCH4III	ACNGT	5 cut(s)	64, 670, 811, 851, 1162
HpyCH4IV	ACGT	5 cut(s)	219, 1288, 1585, 1636, 1651
HpyCH4V	TGCA	9 cut(s)	428, 509, 683, 929, 1006, 1022, 1448, 1655, 1739
HpyF10VI	GCNNNNNNNGC	8 cut(s)	407, 643, 655, 971, 1314, 1420, 1445, 1919
HpyF3I	CTNAG	4 cut(s)	348, 375, 393, 1608
HpySE526I	ACGT	5 cut(s)	219, 1288, 1585, 1636, 1651
HspAI	GCGC	2 cut(s)	215, 257
Kpn2I	TCCGGA	1 cut(s)	1283
KpnI	GGTACC	1 cut(s)	881
KspAI	GTTAAC	1 cut(s)	673
Kzo9I	GATC	8 cut(s)	129, 295, 556, 702, 1455, 1555, 1567, 1697
LmnI	GCTCC	5 cut(s)	128, 214, 361, 643, 688
Lsp1109I	GCAGC	5 cut(s)	55, 493, 667, 1308, 1460
LweI	GCATC	2 cut(s)	229, 301
MaeI	CTAG	3 cut(s)	1256, 1424, 1680
MaeII	ACGT	5 cut(s)	219, 1288, 1585, 1636, 1651
MaeIII	GTNAC	5 cut(s)	22, 272, 624, 1138, 1615
MalI	GATC	8 cut(s)	131, 297, 558, 704, 1457, 1557, 1569, 1699
MbiI	CCGCTC	2 cut(s)	123, 649
MboI	GATC	8 cut(s)	129, 295, 556, 702, 1455, 1555, 1567, 1697
MflI	RGATCY	1 cut(s)	1567
MhlI	GDGCHC	2 cut(s)	528, 1651
MlsI	TGGCCA	4 cut(s)	53, 942, 1016, 1148
MluCI	AATT	4 cut(s)	369, 1368, 1629, 1871
MluNI	TGGCCA	4 cut(s)	53, 942, 1016, 1148
MlyI	GAGTC	4 cut(s)	146, 293, 639, 739
MmeI	TCCRAC	2 cut(s)	168, 1060
Mox20I	TGGCCA	4 cut(s)	53, 942, 1016, 1148
Mph1103I	ATGCAT	1 cut(s)	1008
MroI	TCCGGA	1 cut(s)	1283
MroXI	GAANNNNTTC	1 cut(s)	1815
MscI	TGGCCA	4 cut(s)	53, 942, 1016, 1148
MseI	TTAA	4 cut(s)	438, 672, 1028, 1716
MslI	CAYNNNNRTG	3 cut(s)	498, 1353, 1654
Msp20I	TGGCCA	4 cut(s)	53, 942, 1016, 1148
MspA1I	CMGCKG	1 cut(s)	308
MspI	CCGG	6 cut(s)	42, 97, 112, 1284, 1576, 1645
MspR9I	CCNGG	4 cut(s)	936, 1576, 1577, 1646
Mva1269I	GAATGC	1 cut(s)	494
MvaI	CCWGG	1 cut(s)	936
MwoI	GCNNNNNNNGC	8 cut(s)	407, 643, 655, 971, 1314, 1420, 1445, 1919
NciI	CCSGG	3 cut(s)	1576, 1577, 1646
NcoI	CCATGG	1 cut(s)	770
NdeI	CATATG	2 cut(s)	1114, 1171
NdeII	GATC	8 cut(s)	129, 295, 556, 702, 1455, 1555, 1567, 1697
NlaIV	GGNNCC	8 cut(s)	210, 325, 645, 879, 1531, 1581, 1661, 1824
NmuCI	GTSAC	4 cut(s)	22, 272, 624, 1615
NsbI	TGCGCA	1 cut(s)	258
NsiI	ATGCAT	1 cut(s)	1008
NspI	RCATGY	2 cut(s)	955, 1321
PaeI	GCATGC	1 cut(s)	1321
PagI	TCATGA	1 cut(s)	1848
PceI	AGGCCT	2 cut(s)	1034, 1746
PcsI	WCGNNNNNNNCGW	1 cut(s)	141
PctI	GAATGC	1 cut(s)	494
PdmI	GAANNNNTTC	1 cut(s)	1815
PfeI	GAWTC	4 cut(s)	106, 1280, 1791, 1845
PflMI	CCANNNNNTGG	3 cut(s)	776, 1008, 1079
PkrI	GCNGC	7 cut(s)	70, 508, 648, 682, 1110, 1323, 1450
Ple19I	CGATCG	1 cut(s)	132
PleI	GAGTC	4 cut(s)	145, 292, 638, 739
PmaCI	CACGTG	1 cut(s)	1652
PmlI	CACGTG	1 cut(s)	1652
PpsI	GAGTC	4 cut(s)	145, 292, 638, 739
Ppu21I	YACGTR	1 cut(s)	1652
PpuMI	RGGWCCY	1 cut(s)	1822
Psp5II	RGGWCCY	1 cut(s)	1822
Psp6I	CCWGG	1 cut(s)	934
PspCI	CACGTG	1 cut(s)	1652
PspFI	CCCAGC	1 cut(s)	776
PspGI	CCWGG	1 cut(s)	934
PspN4I	GGNNCC	8 cut(s)	210, 325, 645, 879, 1531, 1581, 1661, 1824
PspPI	GGNCC	4 cut(s)	552, 1529, 1560, 1822
PspPPI	RGGWCCY	1 cut(s)	1822
PsuI	RGATCY	1 cut(s)	1567
PvuI	CGATCG	1 cut(s)	132
PvuII	CAGCTG	1 cut(s)	308
RsaI	GTAC	5 cut(s)	12, 879, 1060, 1313, 1359
RsaNI	GTAC	5 cut(s)	11, 878, 1059, 1312, 1358
RseI	CAYNNNNRTG	3 cut(s)	498, 1353, 1654
SaqAI	TTAA	4 cut(s)	438, 672, 1028, 1716
SatI	GCNGC	7 cut(s)	69, 507, 647, 681, 1109, 1322, 1449
Sau3AI	GATC	8 cut(s)	129, 295, 556, 702, 1455, 1555, 1567, 1697
Sau96I	GGNCC	4 cut(s)	552, 1529, 1560, 1822
SchI	GAGTC	4 cut(s)	146, 293, 639, 739
ScrFI	CCNGG	4 cut(s)	936, 1576, 1577, 1646
SduI	GDGCHC	2 cut(s)	528, 1651
SfaNI	GCATC	2 cut(s)	229, 301
SinI	GGWCC	3 cut(s)	552, 1529, 1822
SmaI	CCCGGG	1 cut(s)	1577
SmiMI	CAYNNNNRTG	3 cut(s)	498, 1353, 1654
SmlI	CTYRAG	1 cut(s)	288
SmoI	CTYRAG	1 cut(s)	288
SphI	GCATGC	1 cut(s)	1321
Sse9I	AATT	4 cut(s)	369, 1368, 1629, 1871
SseBI	AGGCCT	2 cut(s)	1034, 1746
SsiI	CCGC	7 cut(s)	5, 121, 327, 647, 923, 1108, 1315
SspMI	CTAG	3 cut(s)	1256, 1424, 1680
StuI	AGGCCT	2 cut(s)	1034, 1746
StyD4I	CCNGG	4 cut(s)	934, 1574, 1575, 1644
StyI	CCWWGG	1 cut(s)	770
TaaI	ACNGT	5 cut(s)	64, 670, 811, 851, 1162
TaiI	ACGT	5 cut(s)	222, 1291, 1588, 1639, 1654
TaqI	TCGA	1 cut(s)	132
TasI	AATT	4 cut(s)	369, 1368, 1629, 1871
TauI	GCSGC	2 cut(s)	649, 1111
TfiI	GAWTC	4 cut(s)	106, 1280, 1791, 1845
Tru1I	TTAA	4 cut(s)	438, 672, 1028, 1716
Tru9I	TTAA	4 cut(s)	438, 672, 1028, 1716
TscAI	CASTG	4 cut(s)	337, 604, 856, 1149
TseFI	GTSAC	4 cut(s)	22, 272, 624, 1615
TseI	GCWGC	5 cut(s)	68, 506, 680, 1321, 1448
Tsp45I	GTSAC	4 cut(s)	22, 272, 624, 1615
TspGWI	ACGGA	4 cut(s)	741, 1397, 1457, 1777
TspMI	CCCGGG	1 cut(s)	1575
TspRI	CASTG	4 cut(s)	337, 604, 856, 1149
Van91I	CCANNNNNTGG	3 cut(s)	776, 1008, 1079
VpaK11BI	GGWCC	3 cut(s)	552, 1529, 1822
XapI	RAATTY	2 cut(s)	369, 1871
XceI	RCATGY	2 cut(s)	955, 1321
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1009, 1540
XmaI	CCCGGG	1 cut(s)	1575
XmnI	GAANNNNTTC	1 cut(s)	1815
XspI	CTAG	3 cut(s)	1256, 1424, 1680
Zsp2I	ATGCAT	1 cut(s)	1008

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.