MD16G1158100.v1.1

Chitinase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012820
Physical Location & Seq
Forward (+)
12663694 .. 12666336
2643 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1158100.v1.1.491

Sequence Viewer

Length: 984 bp
ATGGAGAAAAAATGGCTTCTGCTTTTCTCAATGGCAGCTGCAATGCTGCTAGCGATCAACGTTGTGAACGGTCAAGAGGATTCAGCGGTGAAGCCGTTGGTGAAGATAGTGAAGGGCAAGAAGGTGTGCGACAAGGGGTGGGAGTGTAAAGGATGGTCTGTTTATTGTTGTAACCAGACCATTTCTGACTACTTCCAAGCTTACCACTTCGAGGACCTTTTCTCCAAGCGTAACTCGCCGGTGGCGCACGCCGTGGGATTCTGGGACTACCACTCTTTCATCACCGCTGCTGCCGATTACCAGCCTCATGGATTCGGTACCACAGGTGGCAAGCTCCAGGGGATGAAGGAAGTTGCTGCTTTTCTTGGGCATGTTGGCAGCAAAACTACGTGTGGCTATGGGGTTGCCACCGGAGGACCATTGGCTTGGGGTCTATGCTACAACAAGGAAATGAGCCCTAGCCAGTTATACTGTGATGACTACTACAAATACACTTACCCCTGCTCTCCTGGTGCTTCATACCATGGCCGTGGTGCCTTGCCACTCTATTGGAACTACAACTATGGAGAAACAGGTCAAGCCCTAAAGGTGGACTTATTAAACCATCCAGAATACATAGAACAAAATGCAACCCTAGCCTTCCAGGCTGCAATCTGGAGGTGGATGACGCCAGTAAAGAAGAACATCCCTTCAGTCCACGATGTCTTTGTCGGCAAATGGAAGCCAACCAAGAACGACACTTTATCCAAAAGAGTTCCTGGCTTTGGCACCACCATCAATGTGCTCTATGGAGACCAAGTCTGTGGCCAAGGTGACGTTGACTCCATGAACAACATCGTCTCCCATTACCTGTATTACCTTGACAAAATCGGGGTTGGCCGCGAAGAGGCTGGACCTCATGACGTGCTCAGCTGCGCTGAACAGAAGGCTTTTCAACCTTCATCTTCTTCGTCTTCGTCTTCGTCTTCATCTTCATCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_19

Protein Analysis

328

Amino Acids

36.05

Weight (kDa)

7.55

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_19 PF00182 69 - 295 3.3e-60 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012820)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16920 AT3G16920
fragaria_vesca FvH4_4g19370
malus_domestica MD16G1158100.v1.1
prunus_persica Prupe.1G122900_v2.0.a1
pyrus_communis pycom13g13460 pycom16g13300
rosa_chinensis RchiOBHm_Chr4g0424411
rosa_laevigata RLG00000007477
rosa_multiflora Rmu_sc0003567.1_g000015
rosa_roxburghii Rroxscaffold_5G00366350
rosa_rugosa Rorug04G0197600
rosa_samantha Rh4AG279500 Rh4BG258800 Rh4BG284900 Rh4CG270100 Rh4DG253300
rosa_wichuraiana Rw4G021870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 317
AccB1I GGYRCC 3 cut(s) 317, 533, 767
AccII CGCG 1 cut(s) 882
AciI CCGC 3 cut(s) 86, 285, 880
AclI AACGTT 1 cut(s) 60
AcoI YGGCCR 3 cut(s) 526, 805, 877
AcuI CTGAAG 1 cut(s) 675
AcyI GRCGYC 1 cut(s) 668
AdeI CACNNNGTG 1 cut(s) 253
AfaI GTAC 1 cut(s) 319
AfiI CCNNNNNNNGG 4 cut(s) 211, 589, 764, 886
AflIII ACRYGT 1 cut(s) 389
AgsI TTSAA 1 cut(s) 935
AjiI CACGTC 1 cut(s) 904
AjnI CCWGG 4 cut(s) 336, 508, 642, 757
AluBI AGCT 4 cut(s) 38, 200, 334, 912
AluI AGCT 4 cut(s) 38, 200, 334, 912
Alw21I GWGCWC 2 cut(s) 786, 909
Alw26I GTCTC 2 cut(s) 786, 844
AoxI GGCC 3 cut(s) 526, 805, 877
ApeKI GCWGC 9 cut(s) 35, 38, 46, 287, 290, 356, 378, 647, 912
Asp718I GGTACC 1 cut(s) 317
AspLEI GCGC 2 cut(s) 247, 917
AspS9I GGNCC 3 cut(s) 214, 416, 893
AsuHPI GGTGA 4 cut(s) 100, 112, 274, 824
AsuNHI GCTAGC 1 cut(s) 49
AvaII GGWCC 3 cut(s) 214, 416, 893
BalI TGGCCA 1 cut(s) 807
BanI GGYRCC 3 cut(s) 317, 533, 767
BanII GRGCYC 1 cut(s) 458
BbsI GAAGAC 3 cut(s) 945, 951, 957
Bbv12I GWGCWC 2 cut(s) 786, 909
BbvI GCAGC 9 cut(s) 25, 33, 47, 274, 277, 343, 390, 634, 899
BccI CCATC 3 cut(s) 147, 612, 782
BceAI ACGGC 3 cut(s) 79, 236, 513
BciT130I CCWGG 4 cut(s) 338, 510, 644, 759
BcoDI GTCTC 2 cut(s) 786, 844
BfaI CTAG 3 cut(s) 50, 459, 635
BglI GCCNNNNNGGC 1 cut(s) 644
BlpI GCTNAGC 1 cut(s) 908
Bme1390I CCNGG 4 cut(s) 338, 510, 644, 759
Bme18I GGWCC 3 cut(s) 214, 416, 893
BmgBI CACGTC 1 cut(s) 904
BmgT120I GGNCC 3 cut(s) 214, 416, 893
BmiI GGNNCC 3 cut(s) 319, 535, 769
BmrFI CCNGG 4 cut(s) 338, 510, 644, 759
BmtI GCTAGC 1 cut(s) 53
BpiI GAAGAC 3 cut(s) 945, 951, 957
BpmI CTGGAG 2 cut(s) 320, 676
Bpu1102I GCTNAGC 1 cut(s) 908
BsaAI YACGTR 1 cut(s) 390
BsaHI GRCGYC 1 cut(s) 668
BsaI GGTCTC 1 cut(s) 786
BsaJI CCNNGG 5 cut(s) 252, 337, 523, 529, 808
BsaWI WCCGGW 1 cut(s) 410
BsaXI ACNNNNNCTCC 8 cut(s) 206, 236, 783, 806, 813, 824, 836, 854
Bsc4I CCNNNNNNNGG 4 cut(s) 211, 589, 764, 886
Bse118I RCCGGY 1 cut(s) 238
Bse1I ACTGG 2 cut(s) 463, 671
Bse3DI GCAATG 1 cut(s) 48
BseBI CCWGG 4 cut(s) 338, 510, 644, 759
BseDI CCNNGG 5 cut(s) 252, 337, 523, 529, 808
BseGI GGATG 5 cut(s) 158, 348, 604, 669, 684
BseLI CCNNNNNNNGG 4 cut(s) 211, 589, 764, 886
BseMI GCAATG 1 cut(s) 48
BseMII CTCAG 1 cut(s) 922
BseNI ACTGG 2 cut(s) 463, 671
BseXI GCAGC 9 cut(s) 25, 33, 47, 274, 277, 343, 390, 634, 899
Bsh1236I CGCG 1 cut(s) 882
BshFI GGCC 3 cut(s) 528, 807, 879
BshNI GGYRCC 3 cut(s) 317, 533, 767
BsiHKAI GWGCWC 2 cut(s) 786, 909
BsiSI CCGG 2 cut(s) 239, 411
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 4 cut(s) 211, 589, 764, 886
BsmAI GTCTC 2 cut(s) 786, 844
BsmBI CGTCTC 1 cut(s) 844
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 3 cut(s) 528, 807, 879
Bso31I GGTCTC 1 cut(s) 786
Bsp1286I GDGCHC 3 cut(s) 458, 786, 909
Bsp143I GATC 1 cut(s) 54
Bsp1720I GCTNAGC 1 cut(s) 908
Bsp19I CCATGG 1 cut(s) 523
BspACI CCGC 3 cut(s) 86, 285, 880
BspANI GGCC 3 cut(s) 528, 807, 879
BspCNI CTCAG 1 cut(s) 921
BspFNI CGCG 1 cut(s) 882
BspHI TCATGA 1 cut(s) 898
BspLI GGNNCC 3 cut(s) 319, 535, 769
BspOI GCTAGC 1 cut(s) 53
BspT107I GGYRCC 3 cut(s) 317, 533, 767
BspTNI GGTCTC 1 cut(s) 786
BsrDI GCAATG 1 cut(s) 48
BsrFI RCCGGY 1 cut(s) 238
BsrI ACTGG 2 cut(s) 463, 671
BssAI RCCGGY 1 cut(s) 238
BssECI CCNNGG 5 cut(s) 252, 337, 523, 529, 808
BssMI GATC 1 cut(s) 54
BssNI GRCGYC 1 cut(s) 668
BssT1I CCWWGG 2 cut(s) 523, 808
Bst2UI CCWGG 4 cut(s) 338, 510, 644, 759
Bst4CI ACNGT 2 cut(s) 71, 473
Bst6I CTCTTC 1 cut(s) 879
BstACI GRCGYC 1 cut(s) 668
BstBAI YACGTR 1 cut(s) 390
BstC8I GCNNGC 3 cut(s) 51, 249, 332
BstDEI CTNAG 1 cut(s) 908
BstDSI CCRYGG 3 cut(s) 252, 523, 529
BstF5I GGATG 5 cut(s) 158, 348, 604, 669, 684
BstFNI CGCG 1 cut(s) 882
BstHHI GCGC 2 cut(s) 247, 917
BstKTI GATC 1 cut(s) 57
BstMAI GTCTC 2 cut(s) 786, 844
BstMBI GATC 1 cut(s) 54
BstMWI GCNNNNNNNGC 4 cut(s) 235, 244, 635, 644
BstNI CCWGG 4 cut(s) 338, 510, 644, 759
BstNSI RCATGY 1 cut(s) 374
BstSCI CCNGG 4 cut(s) 336, 508, 642, 757
BstUI CGCG 1 cut(s) 882
BstV1I GCAGC 9 cut(s) 25, 33, 47, 274, 277, 343, 390, 634, 899
BstV2I GAAGAC 3 cut(s) 945, 951, 957
BstXI CCANNNNNNTGG 5 cut(s) 308, 426, 530, 549, 803
BsuRI GGCC 3 cut(s) 528, 807, 879
BtgI CCRYGG 3 cut(s) 252, 523, 529
BtrI CACGTC 1 cut(s) 904
BtsCI GGATG 5 cut(s) 158, 348, 604, 669, 684
Cac8I GCNNGC 3 cut(s) 51, 249, 332
CciI TCATGA 1 cut(s) 898
CfoI GCGC 2 cut(s) 247, 917
Cfr10I RCCGGY 1 cut(s) 238
Cfr13I GGNCC 3 cut(s) 214, 416, 893
CseI GACGC 1 cut(s) 676
Csp6I GTAC 1 cut(s) 318
CviAII CATG 5 cut(s) 308, 371, 524, 826, 899
CviQI GTAC 1 cut(s) 318
DdeI CTNAG 1 cut(s) 908
DpnI GATC 1 cut(s) 56
DpnII GATC 1 cut(s) 54
DraIII CACNNNGTG 1 cut(s) 253
EaeI YGGCCR 3 cut(s) 526, 805, 877
Eam1104I CTCTTC 1 cut(s) 879
EarI CTCTTC 1 cut(s) 879
Eco130I CCWWGG 2 cut(s) 523, 808
Eco24I GRGCYC 1 cut(s) 458
Eco31I GGTCTC 1 cut(s) 786
Eco47I GGWCC 3 cut(s) 214, 416, 893
Eco57I CTGAAG 1 cut(s) 675
EcoO109I RGGNCCY 1 cut(s) 214
EcoRII CCWGG 4 cut(s) 336, 508, 642, 757
EcoT14I CCWWGG 2 cut(s) 523, 808
EcoT38I GRGCYC 1 cut(s) 458
ErhI CCWWGG 2 cut(s) 523, 808
Esp3I CGTCTC 1 cut(s) 844
FaeI CATG 5 cut(s) 311, 374, 527, 829, 902
FalI AAGNNNNNCTT 2 cut(s) 578, 610
FaqI GGGAC 1 cut(s) 278
FatI CATG 5 cut(s) 307, 370, 523, 825, 898
FokI GGATG 5 cut(s) 165, 355, 591, 671, 676
FriOI GRGCYC 1 cut(s) 458
FspBI CTAG 3 cut(s) 50, 459, 635
GlaI GCGC 2 cut(s) 246, 916
GsuI CTGGAG 2 cut(s) 320, 676
HaeIII GGCC 3 cut(s) 528, 807, 879
HapII CCGG 2 cut(s) 239, 411
HgaI GACGC 1 cut(s) 676
HhaI GCGC 2 cut(s) 247, 917
Hin1I GRCGYC 1 cut(s) 668
Hin1II CATG 5 cut(s) 311, 374, 527, 829, 902
Hin6I GCGC 2 cut(s) 245, 915
HinP1I GCGC 2 cut(s) 245, 915
HincII GTYRAC 1 cut(s) 820
HindII GTYRAC 1 cut(s) 820
HindIII AAGCTT 1 cut(s) 198
HinfI GANTC 4 cut(s) 80, 258, 312, 821
HpaII CCGG 2 cut(s) 239, 411
HphI GGTGA 4 cut(s) 100, 112, 274, 824
Hpy166II GTNNAC 4 cut(s) 67, 592, 697, 820
Hpy188I TCNGA 1 cut(s) 187
Hpy188III TCNNGA 5 cut(s) 74, 608, 655, 899, 981
Hpy8I GTNNAC 4 cut(s) 67, 592, 697, 820
HpyAV CCTTC 7 cut(s) 106, 115, 340, 649, 699, 919, 948
HpyCH4III ACNGT 2 cut(s) 71, 473
HpyCH4IV ACGT 4 cut(s) 60, 389, 816, 903
HpyCH4V TGCA 3 cut(s) 41, 629, 650
HpyF10VI GCNNNNNNNGC 4 cut(s) 235, 244, 635, 644
HpyF3I CTNAG 1 cut(s) 908
HpySE526I ACGT 4 cut(s) 60, 389, 816, 903
Hsp92I GRCGYC 1 cut(s) 668
Hsp92II CATG 5 cut(s) 311, 374, 527, 829, 902
HspAI GCGC 2 cut(s) 245, 915
KpnI GGTACC 1 cut(s) 321
Kzo9I GATC 1 cut(s) 54
LmnI GCTCC 1 cut(s) 339
Lsp1109I GCAGC 9 cut(s) 25, 33, 47, 274, 277, 343, 390, 634, 899
MaeI CTAG 3 cut(s) 50, 459, 635
MaeII ACGT 4 cut(s) 60, 389, 816, 903
MaeIII GTNAC 3 cut(s) 170, 230, 812
MalI GATC 1 cut(s) 56
MboI GATC 1 cut(s) 54
MhlI GDGCHC 3 cut(s) 458, 786, 909
MlsI TGGCCA 1 cut(s) 807
MluNI TGGCCA 1 cut(s) 807
MlyI GAGTC 1 cut(s) 815
MnlI CCTC 7 cut(s) 70, 205, 315, 407, 651, 880, 906
Mox20I TGGCCA 1 cut(s) 807
MscI TGGCCA 1 cut(s) 807
MseI TTAA 1 cut(s) 599
MslI CAYNNNNRTG 2 cut(s) 528, 779
Msp20I TGGCCA 1 cut(s) 807
MspA1I CMGCKG 4 cut(s) 38, 86, 287, 912
MspI CCGG 2 cut(s) 239, 411
MspR9I CCNGG 4 cut(s) 338, 510, 644, 759
MvaI CCWGG 4 cut(s) 338, 510, 644, 759
MvnI CGCG 1 cut(s) 882
MwoI GCNNNNNNNGC 4 cut(s) 235, 244, 635, 644
NcoI CCATGG 1 cut(s) 523
NdeII GATC 1 cut(s) 54
NheI GCTAGC 1 cut(s) 49
NlaIII CATG 5 cut(s) 311, 374, 527, 829, 902
NlaIV GGNNCC 3 cut(s) 319, 535, 769
NmuCI GTSAC 1 cut(s) 812
NspI RCATGY 1 cut(s) 374
PagI TCATGA 1 cut(s) 898
PfeI GAWTC 3 cut(s) 80, 258, 312
PflFI GACNNNGTC 1 cut(s) 797
PleI GAGTC 1 cut(s) 815
PpsI GAGTC 1 cut(s) 815
Ppu21I YACGTR 1 cut(s) 390
PpuMI RGGWCCY 1 cut(s) 214
Psp1406I AACGTT 1 cut(s) 60
Psp5II RGGWCCY 1 cut(s) 214
Psp6I CCWGG 4 cut(s) 336, 508, 642, 757
PspGI CCWGG 4 cut(s) 336, 508, 642, 757
PspN4I GGNNCC 3 cut(s) 319, 535, 769
PspPI GGNCC 3 cut(s) 214, 416, 893
PspPPI RGGWCCY 1 cut(s) 214
PsyI GACNNNGTC 1 cut(s) 797
PvuII CAGCTG 2 cut(s) 38, 912
RsaI GTAC 1 cut(s) 319
RsaNI GTAC 1 cut(s) 318
RseI CAYNNNNRTG 2 cut(s) 528, 779
SaqAI TTAA 1 cut(s) 599
Sau3AI GATC 1 cut(s) 54
Sau96I GGNCC 3 cut(s) 214, 416, 893
SchI GAGTC 1 cut(s) 815
ScrFI CCNGG 4 cut(s) 338, 510, 644, 759
SduI GDGCHC 3 cut(s) 458, 786, 909
SgrAI CRCCGGYG 1 cut(s) 238
SinI GGWCC 3 cut(s) 214, 416, 893
SmiMI CAYNNNNRTG 2 cut(s) 528, 779
SsiI CCGC 3 cut(s) 86, 285, 880
SspMI CTAG 3 cut(s) 50, 459, 635
StyD4I CCNGG 4 cut(s) 336, 508, 642, 757
StyI CCWWGG 2 cut(s) 523, 808
TaaI ACNGT 2 cut(s) 71, 473
TaiI ACGT 4 cut(s) 63, 392, 819, 906
TaqI TCGA 1 cut(s) 210
TauI GCSGC 1 cut(s) 882
TfiI GAWTC 3 cut(s) 80, 258, 312
Tru1I TTAA 1 cut(s) 599
Tru9I TTAA 1 cut(s) 599
TseFI GTSAC 1 cut(s) 812
TseI GCWGC 9 cut(s) 35, 38, 46, 287, 290, 356, 378, 647, 912
Tsp45I GTSAC 1 cut(s) 812
TspDTI ATGAA 7 cut(s) 268, 359, 507, 842, 930, 957, 963
Tth111I GACNNNGTC 1 cut(s) 797
VpaK11BI GGWCC 3 cut(s) 214, 416, 893
XceI RCATGY 1 cut(s) 374
XspI CTAG 3 cut(s) 50, 459, 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.