RchiOBHm_Chr4g0424411

Chitinase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0012820
Physical Location & Seq
Reverse (-)
50024262 .. 50026374
2113 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39368

Sequence Viewer

Length: 972 bp
ATGGAGGCCAAGTGGATTCTTGTTCTTTTCTCAATCATCCTCGTAGTGCAAAATGTGAATGGTGAAGATGAGTCATTATCATCGTCTGTGAAGCCATTGGTGAAGGTAGTAAAGGGCAAGAAGCTGTGTGACAAGGGTTGGGAGTGTAAGGGATGGTCTGCTTATTGTTGTAACCAAACCATTTCAGACTATTTTCAGTCTTACCAATTTGAGGACCTTTTCTCTAAGCGCAACTCGCCTGTGGCGCATGCGGTGGATTTCTGGGACTACCATTCTTTCATTACTGCTGCCGCTCAGTACCAGCCTCATGGCTTTGGTACCACAGGAGGCAAGTTCCAGGGGATGAAGGAAGTTGCGGCTTTTCTTGGCCATGTTGGCAGCAAAACTTCATGTGGATATGGGGTAGCTACAGGAGGACCATTGGCTTGGGGTCTATGCTACAACAAGGAGATGAGTCCTGACAAGTTATACTGTGATGACTATTTCAAATACACTTATCCTTGTGCTCCTGGAGCTTCATACCACGGCCGTGGTGCCTTGCCACTCTACTGGAACTACAACTATGGAGAAGCAGGAGAAGCCCTGAAGGTGGACTTGTTGAACCATCCAGAATATATAGAACAGAATGCTACTCTGGCCTTTCAGGCTGCAATTTGGAGGTGGATGACTCCAGTGAAGAAGAACCAGCCTTCAGCCCATGAAGTCTTTGTTGGAAAGTGGAAACCTACCAAGAATGACACATTGGCCAAAAGGGTTCCTGGATTTGGCACAACCATAAATGTTCTCTATGGGGATCAAGCTTGTGGTCAAGGTGATGTTGACTCCATGAACAACCCTATCTCGCATTACCTGTATTACCTTGATCTTATTGGTGTTGGCCGAGAAGAGGCAGGTCCTCATGATGTGCTCAGCTGCGCTGAGCAAGAACCTTTCGAGCGTTCCTCTTCTACTTCATCATCTTCATCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_19

Protein Analysis

323

Amino Acids

35.81

Weight (kDa)

5.99

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_19 PF00182 69 - 295 1.3e-61 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012820)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16920 AT3G16920
fragaria_vesca FvH4_4g19370
malus_domestica MD16G1158100.v1.1
prunus_persica Prupe.1G122900_v2.0.a1
pyrus_communis pycom13g13460 pycom16g13300
rosa_chinensis RchiOBHm_Chr4g0424411
rosa_laevigata RLG00000007477
rosa_multiflora Rmu_sc0003567.1_g000015
rosa_roxburghii Rroxscaffold_5G00366350
rosa_rugosa Rorug04G0197600
rosa_samantha Rh4AG279500 Rh4BG258800 Rh4BG284900 Rh4CG270100 Rh4DG253300
rosa_wichuraiana Rw4G021870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 881
Acc65I GGTACC 1 cut(s) 317
AccB1I GGYRCC 2 cut(s) 317, 533
AccBSI CCGCTC 1 cut(s) 293
AciI CCGC 3 cut(s) 251, 291, 356
AclWI GGATC 1 cut(s) 801
AcoI YGGCCR 4 cut(s) 367, 526, 744, 877
AcuI CTGAAG 2 cut(s) 605, 675
AfaI GTAC 2 cut(s) 299, 319
AfiI CCNNNNNNNGG 4 cut(s) 211, 589, 764, 886
AgsI TTSAA 2 cut(s) 487, 601
AjnI CCWGG 3 cut(s) 336, 508, 757
AjuI GAANNNNNNNTTGG 4 cut(s) 693, 725, 725, 757
AleI CACNNNNGTG 1 cut(s) 528
AluBI AGCT 5 cut(s) 124, 407, 515, 800, 912
AluI AGCT 5 cut(s) 124, 407, 515, 800, 912
Alw21I GWGCWC 2 cut(s) 508, 909
AlwI GGATC 1 cut(s) 801
AoxI GGCC 6 cut(s) 6, 367, 526, 636, 744, 877
ApeKI GCWGC 4 cut(s) 287, 378, 647, 912
Asp718I GGTACC 1 cut(s) 317
AspLEI GCGC 3 cut(s) 231, 247, 917
AspS9I GGNCC 3 cut(s) 214, 416, 893
AsuHPI GGTGA 3 cut(s) 74, 112, 824
AvaII GGWCC 3 cut(s) 214, 416, 893
BalI TGGCCA 2 cut(s) 369, 746
BanI GGYRCC 2 cut(s) 317, 533
Bbv12I GWGCWC 2 cut(s) 508, 909
BbvI GCAGC 4 cut(s) 274, 390, 634, 899
BccI CCATC 2 cut(s) 147, 612
BceAI ACGGC 2 cut(s) 513, 541
BciT130I CCWGG 3 cut(s) 338, 510, 759
BfmI CTRYAG 1 cut(s) 408
BfuAI ACCTGC 1 cut(s) 881
BglI GCCNNNNNGGC 2 cut(s) 375, 644
BisI GCNGC 6 cut(s) 288, 291, 357, 379, 648, 913
BlpI GCTNAGC 2 cut(s) 908, 918
BlsI GCNGC 6 cut(s) 289, 292, 358, 380, 649, 914
Bme1390I CCNGG 3 cut(s) 338, 510, 759
Bme18I GGWCC 3 cut(s) 214, 416, 893
BmgT120I GGNCC 3 cut(s) 214, 416, 893
BmiI GGNNCC 3 cut(s) 319, 535, 756
BmrFI CCNGG 3 cut(s) 338, 510, 759
BplI GAGNNNNNCTC 2 cut(s) 926, 958
BpmI CTGGAG 2 cut(s) 531, 654
Bpu1102I GCTNAGC 2 cut(s) 908, 918
BsaJI CCNNGG 3 cut(s) 337, 523, 529
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 4 cut(s) 211, 589, 764, 886
Bse1I ACTGG 2 cut(s) 554, 671
BseBI CCWGG 3 cut(s) 338, 510, 759
BseDI CCNNGG 3 cut(s) 337, 523, 529
BseGI GGATG 5 cut(s) 36, 158, 348, 604, 669
BseLI CCNNNNNNNGG 4 cut(s) 211, 589, 764, 886
BseMII CTCAG 3 cut(s) 308, 909, 922
BseNI ACTGG 2 cut(s) 554, 671
BseX3I CGGCCG 1 cut(s) 526
BseXI GCAGC 4 cut(s) 274, 390, 634, 899
Bsh1285I CGRYCG 1 cut(s) 529
BshFI GGCC 6 cut(s) 8, 369, 528, 638, 746, 879
BshNI GGYRCC 2 cut(s) 317, 533
BsiEI CGRYCG 1 cut(s) 529
BsiHKAI GWGCWC 2 cut(s) 508, 909
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 4 cut(s) 211, 589, 764, 886
BsmFI GGGAC 1 cut(s) 278
BsmI GAATGC 1 cut(s) 631
BsnI GGCC 6 cut(s) 8, 369, 528, 638, 746, 879
Bsp1286I GDGCHC 2 cut(s) 508, 909
Bsp143I GATC 2 cut(s) 793, 862
Bsp1720I GCTNAGC 2 cut(s) 908, 918
BspACI CCGC 3 cut(s) 251, 291, 356
BspANI GGCC 6 cut(s) 8, 369, 528, 638, 746, 879
BspCNI CTCAG 3 cut(s) 307, 910, 921
BspHI TCATGA 1 cut(s) 898
BspLI GGNNCC 3 cut(s) 319, 535, 756
BspMI ACCTGC 1 cut(s) 881
BspPI GGATC 1 cut(s) 801
BspT107I GGYRCC 2 cut(s) 317, 533
BsrBI CCGCTC 1 cut(s) 293
BsrI ACTGG 2 cut(s) 554, 671
BssECI CCNNGG 3 cut(s) 337, 523, 529
BssMI GATC 2 cut(s) 793, 862
Bst2UI CCWGG 3 cut(s) 338, 510, 759
Bst4CI ACNGT 1 cut(s) 473
Bst6I CTCTTC 2 cut(s) 879, 949
BstC8I GCNNGC 1 cut(s) 249
BstDEI CTNAG 4 cut(s) 225, 294, 908, 918
BstDSI CCRYGG 2 cut(s) 523, 529
BstF5I GGATG 5 cut(s) 36, 158, 348, 604, 669
BstHHI GCGC 3 cut(s) 231, 247, 917
BstKTI GATC 2 cut(s) 796, 865
BstMBI GATC 2 cut(s) 793, 862
BstMCI CGRYCG 1 cut(s) 529
BstMWI GCNNNNNNNGC 7 cut(s) 235, 244, 375, 512, 578, 635, 644
BstNI CCWGG 3 cut(s) 338, 510, 759
BstNSI RCATGY 1 cut(s) 251
BstSCI CCNGG 3 cut(s) 336, 508, 757
BstSFI CTRYAG 1 cut(s) 408
BstV1I GCAGC 4 cut(s) 274, 390, 634, 899
BstXI CCANNNNNNTGG 4 cut(s) 308, 426, 530, 549
BstZI CGGCCG 1 cut(s) 526
BsuRI GGCC 6 cut(s) 8, 369, 528, 638, 746, 879
BtgI CCRYGG 2 cut(s) 523, 529
BtsCI GGATG 5 cut(s) 36, 158, 348, 604, 669
BtsIMutI CAGTG 1 cut(s) 678
BveI ACCTGC 1 cut(s) 881
Cac8I GCNNGC 1 cut(s) 249
CciI TCATGA 1 cut(s) 898
CfoI GCGC 3 cut(s) 231, 247, 917
Cfr13I GGNCC 3 cut(s) 214, 416, 893
Csp6I GTAC 2 cut(s) 298, 318
CviAII CATG 7 cut(s) 248, 308, 371, 390, 698, 826, 899
CviQI GTAC 2 cut(s) 298, 318
DdeI CTNAG 4 cut(s) 225, 294, 908, 918
DpnI GATC 2 cut(s) 795, 864
DpnII GATC 2 cut(s) 793, 862
EaeI YGGCCR 4 cut(s) 367, 526, 744, 877
EagI CGGCCG 1 cut(s) 526
Eam1104I CTCTTC 2 cut(s) 879, 949
EarI CTCTTC 2 cut(s) 879, 949
EclXI CGGCCG 1 cut(s) 526
Eco47I GGWCC 3 cut(s) 214, 416, 893
Eco52I CGGCCG 1 cut(s) 526
Eco57I CTGAAG 2 cut(s) 605, 675
EcoO109I RGGNCCY 2 cut(s) 214, 893
EcoRII CCWGG 3 cut(s) 336, 508, 757
FaeI CATG 7 cut(s) 251, 311, 374, 393, 701, 829, 902
FalI AAGNNNNNCTT 2 cut(s) 578, 610
FaqI GGGAC 1 cut(s) 278
FatI CATG 7 cut(s) 247, 307, 370, 389, 697, 825, 898
Fnu4HI GCNGC 6 cut(s) 288, 291, 357, 379, 648, 913
FokI GGATG 5 cut(s) 23, 165, 355, 591, 676
Fsp4HI GCNGC 6 cut(s) 288, 291, 357, 379, 648, 913
GlaI GCGC 3 cut(s) 230, 246, 916
GluI GCNGC 6 cut(s) 288, 291, 357, 379, 648, 913
GsuI CTGGAG 2 cut(s) 531, 654
HaeIII GGCC 6 cut(s) 8, 369, 528, 638, 746, 879
HhaI GCGC 3 cut(s) 231, 247, 917
Hin1II CATG 7 cut(s) 251, 311, 374, 393, 701, 829, 902
Hin6I GCGC 3 cut(s) 229, 245, 915
HinP1I GCGC 3 cut(s) 229, 245, 915
HincII GTYRAC 1 cut(s) 820
HindII GTYRAC 1 cut(s) 820
HindIII AAGCTT 1 cut(s) 798
HinfI GANTC 5 cut(s) 16, 71, 454, 667, 821
HphI GGTGA 3 cut(s) 74, 112, 824
Hpy166II GTNNAC 2 cut(s) 592, 820
Hpy188I TCNGA 1 cut(s) 187
Hpy188III TCNNGA 4 cut(s) 458, 608, 899, 969
Hpy8I GTNNAC 2 cut(s) 592, 820
HpyAV CCTTC 4 cut(s) 97, 340, 580, 699
HpyCH4III ACNGT 1 cut(s) 473
HpyCH4V TGCA 2 cut(s) 49, 650
HpyF10VI GCNNNNNNNGC 7 cut(s) 235, 244, 375, 512, 578, 635, 644
HpyF3I CTNAG 4 cut(s) 225, 294, 908, 918
Hsp92II CATG 7 cut(s) 251, 311, 374, 393, 701, 829, 902
HspAI GCGC 3 cut(s) 229, 245, 915
KpnI GGTACC 1 cut(s) 321
Kzo9I GATC 2 cut(s) 793, 862
LmnI GCTCC 2 cut(s) 511, 512
Lsp1109I GCAGC 4 cut(s) 274, 390, 634, 899
MaeIII GTNAC 2 cut(s) 128, 170
MalI GATC 2 cut(s) 795, 864
MbiI CCGCTC 1 cut(s) 293
MboI GATC 2 cut(s) 793, 862
MboII GAAGA 7 cut(s) 77, 688, 691, 896, 936, 951, 957
MhlI GDGCHC 2 cut(s) 508, 909
MlsI TGGCCA 2 cut(s) 369, 746
MluCI AATT 2 cut(s) 206, 651
MluNI TGGCCA 2 cut(s) 369, 746
MlyI GAGTC 4 cut(s) 80, 463, 661, 815
MmeI TCCRAC 1 cut(s) 691
MnlI CCTC 9 cut(s) 50, 205, 315, 320, 407, 651, 880, 906, 952
Mox20I TGGCCA 2 cut(s) 369, 746
MscI TGGCCA 2 cut(s) 369, 746
MslI CAYNNNNRTG 1 cut(s) 528
Msp20I TGGCCA 2 cut(s) 369, 746
MspA1I CMGCKG 1 cut(s) 912
MspR9I CCNGG 3 cut(s) 338, 510, 759
Mva1269I GAATGC 1 cut(s) 631
MvaI CCWGG 3 cut(s) 338, 510, 759
MwoI GCNNNNNNNGC 7 cut(s) 235, 244, 375, 512, 578, 635, 644
NdeII GATC 2 cut(s) 793, 862
NlaIII CATG 7 cut(s) 251, 311, 374, 393, 701, 829, 902
NlaIV GGNNCC 3 cut(s) 319, 535, 756
NmeAIII GCCGAG 1 cut(s) 905
NmuCI GTSAC 1 cut(s) 128
NspI RCATGY 1 cut(s) 251
OliI CACNNNNGTG 1 cut(s) 528
PaeI GCATGC 1 cut(s) 251
PagI TCATGA 1 cut(s) 898
PctI GAATGC 1 cut(s) 631
PfeI GAWTC 1 cut(s) 16
PfoI TCCNGGA 2 cut(s) 508, 757
PkrI GCNGC 6 cut(s) 289, 292, 358, 380, 649, 914
PleI GAGTC 4 cut(s) 79, 462, 661, 815
PpsI GAGTC 4 cut(s) 79, 462, 661, 815
PpuMI RGGWCCY 2 cut(s) 214, 893
Psp5II RGGWCCY 2 cut(s) 214, 893
Psp6I CCWGG 3 cut(s) 336, 508, 757
PspGI CCWGG 3 cut(s) 336, 508, 757
PspN4I GGNNCC 3 cut(s) 319, 535, 756
PspPI GGNCC 3 cut(s) 214, 416, 893
PspPPI RGGWCCY 2 cut(s) 214, 893
PvuII CAGCTG 1 cut(s) 912
RsaI GTAC 2 cut(s) 299, 319
RsaNI GTAC 2 cut(s) 298, 318
RseI CAYNNNNRTG 1 cut(s) 528
SatI GCNGC 6 cut(s) 288, 291, 357, 379, 648, 913
Sau3AI GATC 2 cut(s) 793, 862
Sau96I GGNCC 3 cut(s) 214, 416, 893
SchI GAGTC 4 cut(s) 80, 463, 661, 815
ScrFI CCNGG 3 cut(s) 338, 510, 759
SduI GDGCHC 2 cut(s) 508, 909
SfcI CTRYAG 1 cut(s) 408
SinI GGWCC 3 cut(s) 214, 416, 893
SmiMI CAYNNNNRTG 1 cut(s) 528
SphI GCATGC 1 cut(s) 251
Sse9I AATT 2 cut(s) 206, 651
SsiI CCGC 3 cut(s) 251, 291, 356
StyD4I CCNGG 3 cut(s) 336, 508, 757
TaaI ACNGT 1 cut(s) 473
TaqI TCGA 1 cut(s) 933
TasI AATT 2 cut(s) 206, 651
TauI GCSGC 2 cut(s) 293, 359
TfiI GAWTC 1 cut(s) 16
TscAI CASTG 1 cut(s) 678
TseFI GTSAC 1 cut(s) 128
TseI GCWGC 4 cut(s) 287, 378, 647, 912
Tsp45I GTSAC 1 cut(s) 128
TspDTI ATGAA 8 cut(s) 268, 359, 378, 507, 714, 842, 942, 951
TspRI CASTG 1 cut(s) 678
VpaK11BI GGWCC 3 cut(s) 214, 416, 893
XceI RCATGY 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.