Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0009296

Physical Location & Seq

Genomic Coordinates Forward (+)

12718613 .. 12722667

Gene Structure 4055 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1158700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1536 bp

ATGAAGATCAGCATCAATTCGATGGCTCGGCGAGTCGAGGTCGATAATCGCTATCCTCTCCGATTTTACTTCCGAATCGCCGATAATCTTCTCCGACAGGCTAGTATTTACCGGGAGGAGAAAAACATCCTAGATTTGTACGTCATACTAATACGATTCTCAAGTTTGGTGTCTGAAACTATACCGTGTCATCGAGACTACCAGACGTATCCTTCGAAAGAGAAAACTCTATATAGAAAGAAACTGCTAAAAGTACTAGATGAGCTTGAATCTTTAAAGCCGGAAGCCCAGCGCCTAGTGAGCAAACTTAACGAGGCCCAAGCAGTTGCTCAACTACCTCAACTTGAAAGCCTAGAAGGCACTTCAAATGGTTTGGCAACGTCTTCTTTAGAGTGGCCTTCTGTGAACAATAAATCGTTGAGCCTAAGCGTTAGGCAGCCTGGTAGCTTGACATCTCAGTCATCATGGAAGTATAGAAATGATTATTCACAGATATCAACAAATACTACACAAATTGACAAGCAGTTCCAGAAGCTATCTTTTAATCTACCTCTTCCCAATAAAGAGACTTTGTCTAGACACTCATTTTTGGGTCCAAATGGTCTTCAGGGCCAGTGGCGAGGACCTAGTGGAAAAGTTAAGGTTCAGTATCCAACCAGTACTGACTTAATCCCTACGGATAATTCAGACCTGAATCAGGCTGTACCAAATGATATTGTGGCTGTACAAGATGGTGATCCAGGAGGAATTAAATCTACAATGGAATCGGTGCTCTCCTTGGATGATGGTGCATGGCCATGCTCTTCTCAGGAATCAAGTCCTTCATTGATTAATGGAGCAAGGGAAGATCCCTTCGAGTTGGTCATCCGTCAGCCTTCTCCTCCTCCTGTACTTGCTCGAGTGCAGCAGGAATATACTCCAATTCCTCCATCAAAAGTGGCAGATCCAAGGCCAGGACTTGCAAAACCTTCTCTGGATGGAATGCCAAGCTCCAATTCATATCAACATTTACATGTTCCAGTAAAATTGATGGATGATTTCTTGAGATTGGCTAGGGCTAATACAGAAAAAAACTTAGAGACGTGTGGTATTCTTGCTGGTGCATTGAAAAACAAGGTTTTCCATATCACCACGCTAATAGTCCCAAAGCAAGAGTCGACCTCTGATTCGTGCCAAACGTTGAATGAGGAAGAAATATTTGAGGTTCAAGACAGATTATCTCTTTTTCCACTTGGCTGGATTCATACACACCCATCACAGACCTGTTTCATGTCGTCCGTTGATCTGCACACTCACTACTCATACCAGATTATGTTACCAGAAGCAATTGCAATCGTTATGGCTCCAACAGATACATCCAGTCCACACGGCATATTTCATCTATCGGACCCAGATGGTGTATCTGTAATTCGTAACTGTGATCAGCGTGGGTTTCATCCACATGAAGAGCCCTCGGATGGAAACCCAATTTATGAGCATTGTTCTCATGTGTTTATAAACTCCAATTTGAGGATTAATGTGGTCGATCTGCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0006464 GO:0006508 GO:0006807 GO:0006810 GO:0006897 GO:0006996 GO:0007033 GO:0008150 GO:0008152 GO:0009987 GO:0010638 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0016192 GO:0016579 GO:0019538 GO:0031224 GO:0031410 GO:0031982 GO:0032386 GO:0032388 GO:0032879 GO:0032880 GO:0033043 GO:0033157 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043412 GO:0044088 GO:0044090 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0046907 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051049 GO:0051050 GO:0051128 GO:0051130 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051641 GO:0051649 GO:0060341 GO:0065007 GO:0070201 GO:0070536 GO:0070646 GO:0070647 GO:0071108 GO:0071704 GO:0071840 GO:0090087 GO:0090316 GO:0097708 GO:0098657 GO:1901564 GO:1903827 GO:1903829 GO:1904951

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

JAB USP8_dimer

Protein Analysis

512

Amino Acids

57.28

Weight (kDa)

6.03

Isoelectric Point (pI)

43.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
USP8_dimer	PF08969	5 - 94	3.7e-13	USP8 dimerisation domain
JAB	PF01398	333 - 441	6.6e-18	JAB1/Mov34/MPN/PAD-1 ubiquitin protease
Prok-JAB	PF14464	351 - 433	1.8e-06	Prokaryotic homologs of the JAB domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009296)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16144
fragaria_vesca	FvH4_4g19320 FvH4_4g19320 FvH4_4g19320
malus_domestica	MD13G1157700.v1.1 MD16G1158700.v1.1
prunus_persica	Prupe.1G125200_v2.0.a1
pyrus_communis	pycom13g13500 pycom16g13350
rosa_chinensis	RchiOBHm_Chr4g0424341
rosa_laevigata	RLG00000007483
rosa_multiflora	Rmu_sc0005995.1_g000011
rosa_roxburghii	Rroxscaffold_5G00366290
rosa_rugosa	Rorug04G0196100 Rorug04G0196200 Rorug04G0196300 Rorug04G0196400
rosa_samantha	Rh4AG252900 Rh4BG258300 Rh4CG269300 Rh4DG252700
rosa_wichuraiana	Rw4G021810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1496
AasI	GACNNNNNNGTC	1 cut(s)	457
AccI	GTMKAC	1 cut(s)	1157
AciI	CCGC	1 cut(s)	1531
AclI	AACGTT	1 cut(s)	1178
AclWI	GGATC	3 cut(s)	731, 842, 938
AcoI	YGGCCR	1 cut(s)	794
AcuI	CTGAAG	1 cut(s)	590
AfaI	GTAC	6 cut(s)	140, 255, 661, 705, 726, 891
AfiI	CCNNNNNNNGG	4 cut(s)	697, 953, 1457, 1509
AflIII	ACRYGT	2 cut(s)	1012, 1082
AgsI	TTSAA	6 cut(s)	269, 347, 366, 1108, 1183, 1208
AjiI	CACGTC	1 cut(s)	1083
AjnI	CCWGG	3 cut(s)	439, 739, 952
AjuI	GAANNNNNNNTTGG	2 cut(s)	979, 1011
AluBI	AGCT	4 cut(s)	265, 447, 535, 990
AluI	AGCT	4 cut(s)	265, 447, 535, 990
Alw21I	GWGCWC	1 cut(s)	774
Alw26I	GTCTC	3 cut(s)	189, 560, 1073
AlwI	GGATC	3 cut(s)	731, 842, 938
Ama87I	CYCGRG	1 cut(s)	897
AoxI	GGCC	5 cut(s)	315, 395, 610, 794, 950
ApeKI	GCWGC	2 cut(s)	436, 904
ArsI	GACNNNNNNTTYG	6 cut(s)	196, 228, 1139, 1150, 1171, 1182
AseI	ATTAAT	2 cut(s)	831, 1515
AspLEI	GCGC	1 cut(s)	294
AspS9I	GGNCC	5 cut(s)	316, 593, 610, 623, 1387
AsuC2I	CCSGG	1 cut(s)	113
AsuHPI	GGTGA	2 cut(s)	746, 1120
AsuII	TTCGAA	1 cut(s)	215
AvaI	CYCGRG	1 cut(s)	897
AvaII	GGWCC	3 cut(s)	593, 623, 1387
BalI	TGGCCA	1 cut(s)	796
BanII	GRGCYC	1 cut(s)	1452
BbsI	GAAGAC	2 cut(s)	375, 596
Bbv12I	GWGCWC	1 cut(s)	774
BbvI	GCAGC	2 cut(s)	448, 916
BccI	CCATC	9 cut(s)	16, 725, 779, 937, 971, 1024, 1261, 1388, 1451
BceAI	ACGGC	1 cut(s)	1384
BciT130I	CCWGG	3 cut(s)	441, 741, 954
BciVI	GTATCC	2 cut(s)	219, 660
BclI	TGATCA	1 cut(s)	1420
BcnI	CCSGG	1 cut(s)	113
BcoDI	GTCTC	3 cut(s)	189, 560, 1073
BfaI	CTAG	8 cut(s)	102, 131, 257, 296, 353, 576, 627, 1053
BfoI	RGCGCY	1 cut(s)	295
BfuI	GTATCC	2 cut(s)	219, 660
BglI	GCCNNNNNGGC	1 cut(s)	357
BisI	GCNGC	2 cut(s)	437, 905
BlsI	GCNGC	2 cut(s)	438, 906
BmcAI	AGTACT	2 cut(s)	255, 661
Bme1390I	CCNGG	4 cut(s)	113, 441, 741, 954
Bme18I	GGWCC	3 cut(s)	593, 623, 1387
BmeT110I	CYCGRG	1 cut(s)	897
BmgBI	CACGTC	1 cut(s)	1083
BmgT120I	GGNCC	5 cut(s)	316, 593, 610, 623, 1387
BmiI	GGNNCC	3 cut(s)	594, 1344, 1389
BmrFI	CCNGG	4 cut(s)	113, 441, 741, 954
BmsI	GCATC	1 cut(s)	21
BpiI	GAAGAC	2 cut(s)	375, 596
BplI	GAGNNNNNCTC	2 cut(s)	1145, 1177
Bpu10I	CCTNAGC	1 cut(s)	425
Bpu14I	TTCGAA	1 cut(s)	215
BpuEI	CTTGAG	2 cut(s)	145, 1063
BpuMI	CCSGG	1 cut(s)	113
BsaBI	GATNNNNATC	2 cut(s)	11, 735
BsaJI	CCNNGG	3 cut(s)	777, 947, 1452
Bsc4I	CCNNNNNNNGG	4 cut(s)	697, 953, 1457, 1509
Bse1I	ACTGG	4 cut(s)	613, 657, 1019, 1359
Bse8I	GATNNNNATC	2 cut(s)	11, 735
BseBI	CCWGG	3 cut(s)	441, 741, 954
BseDI	CCNNGG	3 cut(s)	777, 947, 1452
BseGI	GGATG	8 cut(s)	126, 787, 864, 982, 1039, 1355, 1435, 1462
BseJI	GATNNNNATC	2 cut(s)	11, 735
BseLI	CCNNNNNNNGG	4 cut(s)	697, 953, 1457, 1509
BseMII	CTCAG	2 cut(s)	470, 821
BseNI	ACTGG	4 cut(s)	613, 657, 1019, 1359
BseRI	GAGGAG	3 cut(s)	131, 870, 873
BseXI	GCAGC	2 cut(s)	448, 916
BseYI	CCCAGC	1 cut(s)	288
BsgI	GTGCAG	2 cut(s)	923, 1271
BshFI	GGCC	5 cut(s)	317, 397, 612, 796, 952
BsiHKAI	GWGCWC	1 cut(s)	774
BsiHKCI	CYCGRG	1 cut(s)	897
BsiSI	CCGG	2 cut(s)	112, 281
BslFI	GGGAC	1 cut(s)	1127
BslI	CCNNNNNNNGG	4 cut(s)	697, 953, 1457, 1509
BsmAI	GTCTC	3 cut(s)	189, 560, 1073
BsmBI	CGTCTC	1 cut(s)	1073
BsmFI	GGGAC	1 cut(s)	1127
BsmI	GAATGC	1 cut(s)	987
BsnI	GGCC	5 cut(s)	317, 397, 612, 796, 952
BsoBI	CYCGRG	1 cut(s)	897
Bsp119I	TTCGAA	1 cut(s)	215
Bsp1286I	GDGCHC	2 cut(s)	774, 1452
Bsp1407I	TGTACA	1 cut(s)	724
Bsp143I	GATC	7 cut(s)	6, 736, 847, 943, 1282, 1420, 1525
BspACI	CCGC	1 cut(s)	1531
BspANI	GGCC	5 cut(s)	317, 397, 612, 796, 952
BspCNI	CTCAG	2 cut(s)	469, 820
BspLI	GGNNCC	3 cut(s)	594, 1344, 1389
BspPI	GGATC	3 cut(s)	731, 842, 938
BspQI	GCTCTTC	2 cut(s)	808, 1440
BspT104I	TTCGAA	1 cut(s)	215
BsrGI	TGTACA	1 cut(s)	724
BsrI	ACTGG	4 cut(s)	613, 657, 1019, 1359
BssECI	CCNNGG	3 cut(s)	777, 947, 1452
BssMI	GATC	7 cut(s)	6, 736, 847, 943, 1282, 1420, 1525
BssT1I	CCWWGG	2 cut(s)	777, 947
Bst2UI	CCWGG	3 cut(s)	441, 741, 954
Bst4CI	ACNGT	2 cut(s)	186, 1418
Bst6I	CTCTTC	3 cut(s)	558, 808, 1440
BstAUI	TGTACA	1 cut(s)	724
BstBI	TTCGAA	1 cut(s)	215
BstDEI	CTNAG	4 cut(s)	425, 456, 807, 1075
BstENI	CCTNNNNNAGG	1 cut(s)	695
BstF5I	GGATG	8 cut(s)	126, 787, 864, 982, 1039, 1355, 1435, 1462
BstH2I	RGCGCY	1 cut(s)	295
BstHHI	GCGC	1 cut(s)	294
BstKTI	GATC	7 cut(s)	9, 739, 850, 946, 1285, 1423, 1528
BstMAI	GTCTC	3 cut(s)	189, 560, 1073
BstMBI	GATC	7 cut(s)	6, 736, 847, 943, 1282, 1420, 1525
BstMWI	GCNNNNNNNGC	2 cut(s)	300, 357
BstNI	CCWGG	3 cut(s)	441, 741, 954
BstNSI	RCATGY	1 cut(s)	1016
BstSCI	CCNGG	4 cut(s)	111, 439, 739, 952
BstV1I	GCAGC	2 cut(s)	448, 916
BstV2I	GAAGAC	2 cut(s)	375, 596
BstX2I	RGATCY	2 cut(s)	847, 943
BstXI	CCANNNNNNTGG	1 cut(s)	1236
BstYI	RGATCY	2 cut(s)	847, 943
BsuI	GTATCC	2 cut(s)	219, 660
BsuRI	GGCC	5 cut(s)	317, 397, 612, 796, 952
BtrI	CACGTC	1 cut(s)	1083
BtsCI	GGATG	8 cut(s)	126, 787, 864, 982, 1039, 1355, 1435, 1462
BtsIMutI	CAGTG	1 cut(s)	620
CfoI	GCGC	1 cut(s)	294
Cfr13I	GGNCC	5 cut(s)	316, 593, 610, 623, 1387
Csp6I	GTAC	6 cut(s)	139, 254, 660, 704, 725, 890
CviAII	CATG	7 cut(s)	465, 792, 798, 1013, 1270, 1442, 1487
CviQI	GTAC	6 cut(s)	139, 254, 660, 704, 725, 890
DdeI	CTNAG	4 cut(s)	425, 456, 807, 1075
DpnI	GATC	7 cut(s)	8, 738, 849, 945, 1284, 1422, 1527
DpnII	GATC	7 cut(s)	6, 736, 847, 943, 1282, 1420, 1525
DraI	TTTAAA	1 cut(s)	276
DrdI	GACNNNNNNGTC	1 cut(s)	457
DseDI	GACNNNNNNGTC	1 cut(s)	457
EaeI	YGGCCR	1 cut(s)	794
Eam1104I	CTCTTC	3 cut(s)	558, 808, 1440
EarI	CTCTTC	3 cut(s)	558, 808, 1440
Eco130I	CCWWGG	2 cut(s)	777, 947
Eco24I	GRGCYC	1 cut(s)	1452
Eco32I	GATATC	1 cut(s)	495
Eco47I	GGWCC	3 cut(s)	593, 623, 1387
Eco57I	CTGAAG	1 cut(s)	590
Eco88I	CYCGRG	1 cut(s)	897
EcoNI	CCTNNNNNAGG	1 cut(s)	695
EcoO109I	RGGNCCY	1 cut(s)	623
EcoRII	CCWGG	3 cut(s)	439, 739, 952
EcoRV	GATATC	1 cut(s)	495
EcoT14I	CCWWGG	2 cut(s)	777, 947
EcoT38I	GRGCYC	1 cut(s)	1452
ErhI	CCWWGG	2 cut(s)	777, 947
Esp3I	CGTCTC	1 cut(s)	1073
FaeI	CATG	7 cut(s)	468, 795, 801, 1016, 1273, 1445, 1490
FaqI	GGGAC	1 cut(s)	1127
FatI	CATG	7 cut(s)	464, 791, 797, 1012, 1269, 1441, 1486
FbaI	TGATCA	1 cut(s)	1420
FblI	GTMKAC	1 cut(s)	1157
Fnu4HI	GCNGC	2 cut(s)	437, 905
FokI	GGATG	8 cut(s)	113, 794, 851, 989, 1046, 1342, 1422, 1469
FriOI	GRGCYC	1 cut(s)	1452
Fsp4HI	GCNGC	2 cut(s)	437, 905
FspBI	CTAG	8 cut(s)	102, 131, 257, 296, 353, 576, 627, 1053
GlaI	GCGC	1 cut(s)	293
GluI	GCNGC	2 cut(s)	437, 905
GsaI	CCCAGC	1 cut(s)	292
HaeII	RGCGCY	1 cut(s)	295
HaeIII	GGCC	5 cut(s)	317, 397, 612, 796, 952
HapII	CCGG	2 cut(s)	112, 281
HhaI	GCGC	1 cut(s)	294
Hin1II	CATG	7 cut(s)	468, 795, 801, 1016, 1273, 1445, 1490
Hin6I	GCGC	1 cut(s)	292
HinP1I	GCGC	1 cut(s)	292
HincII	GTYRAC	1 cut(s)	1158
HindII	GTYRAC	1 cut(s)	1158
HpaII	CCGG	2 cut(s)	112, 281
HphI	GGTGA	2 cut(s)	746, 1120
Hpy166II	GTNNAC	3 cut(s)	406, 1158, 1364
Hpy188I	TCNGA	8 cut(s)	62, 74, 95, 175, 688, 1165, 1387, 1456
Hpy188III	TCNNGA	7 cut(s)	194, 529, 576, 809, 974, 1042, 1208
Hpy8I	GTNNAC	3 cut(s)	406, 1158, 1364
HpyAV	CCTTC	7 cut(s)	222, 350, 408, 831, 862, 885, 978
HpyCH4III	ACNGT	2 cut(s)	186, 1418
HpyCH4IV	ACGT	5 cut(s)	141, 206, 380, 1082, 1178
HpyCH4V	TGCA	6 cut(s)	791, 904, 962, 1103, 1288, 1331
HpyF10VI	GCNNNNNNNGC	2 cut(s)	300, 357
HpyF3I	CTNAG	4 cut(s)	425, 456, 807, 1075
HpySE526I	ACGT	5 cut(s)	141, 206, 380, 1082, 1178
Hsp92II	CATG	7 cut(s)	468, 795, 801, 1016, 1273, 1445, 1490
HspAI	GCGC	1 cut(s)	292
Ksp22I	TGATCA	1 cut(s)	1420
Kzo9I	GATC	7 cut(s)	6, 736, 847, 943, 1282, 1420, 1525
LguI	GCTCTTC	2 cut(s)	808, 1440
LmnI	GCTCC	3 cut(s)	836, 995, 1348
Lsp1109I	GCAGC	2 cut(s)	448, 916
LweI	GCATC	1 cut(s)	21
MaeI	CTAG	8 cut(s)	102, 131, 257, 296, 353, 576, 627, 1053
MaeII	ACGT	5 cut(s)	141, 206, 380, 1082, 1178
MaeIII	GTNAC	2 cut(s)	1314, 1412
MalI	GATC	7 cut(s)	8, 738, 849, 945, 1284, 1422, 1527
MboI	GATC	7 cut(s)	6, 736, 847, 943, 1282, 1420, 1525
MboII	GAAGA	9 cut(s)	16, 80, 375, 545, 596, 795, 857, 1202, 1457
MfeI	CAATTG	1 cut(s)	1326
MflI	RGATCY	2 cut(s)	847, 943
MhlI	GDGCHC	2 cut(s)	774, 1452
MlsI	TGGCCA	1 cut(s)	796
MluNI	TGGCCA	1 cut(s)	796
MlyI	GAGTC	2 cut(s)	42, 1163
MmeI	TCCRAC	3 cut(s)	118, 677, 1370
Mox20I	TGGCCA	1 cut(s)	796
MscI	TGGCCA	1 cut(s)	796
MseI	TTAA	8 cut(s)	275, 309, 543, 639, 668, 750, 831, 1515
MslI	CAYNNNNRTG	3 cut(s)	796, 1011, 1440
Msp20I	TGGCCA	1 cut(s)	796
MspI	CCGG	2 cut(s)	112, 281
MspR9I	CCNGG	4 cut(s)	113, 441, 741, 954
MunI	CAATTG	1 cut(s)	1326
Mva1269I	GAATGC	1 cut(s)	987
MvaI	CCWGG	3 cut(s)	441, 741, 954
MwoI	GCNNNNNNNGC	2 cut(s)	300, 357
NciI	CCSGG	1 cut(s)	113
NdeII	GATC	7 cut(s)	6, 736, 847, 943, 1282, 1420, 1525
NlaIII	CATG	7 cut(s)	468, 795, 801, 1016, 1273, 1445, 1490
NlaIV	GGNNCC	3 cut(s)	594, 1344, 1389
NmeAIII	GCCGAG	1 cut(s)	7
NspI	RCATGY	1 cut(s)	1016
NspV	TTCGAA	1 cut(s)	215
PaeR7I	CTCGAG	1 cut(s)	897
PciI	ACATGT	1 cut(s)	1012
PciSI	GCTCTTC	2 cut(s)	808, 1440
PcsI	WCGNNNNNNNCGW	2 cut(s)	212, 1175
PctI	GAATGC	1 cut(s)	987
PfeI	GAWTC	8 cut(s)	75, 156, 269, 694, 764, 812, 1166, 1240
PflFI	GACNNNGTC	1 cut(s)	571
PfoI	TCCNGGA	1 cut(s)	739
PkrI	GCNGC	2 cut(s)	438, 906
PleI	GAGTC	2 cut(s)	41, 1162
PpsI	GAGTC	2 cut(s)	41, 1162
PpuMI	RGGWCCY	1 cut(s)	623
PscI	ACATGT	1 cut(s)	1012
PshBI	ATTAAT	2 cut(s)	831, 1515
PsiI	TTATAA	1 cut(s)	1496
Psp1406I	AACGTT	1 cut(s)	1178
Psp5II	RGGWCCY	1 cut(s)	623
Psp6I	CCWGG	3 cut(s)	439, 739, 952
PspFI	CCCAGC	1 cut(s)	288
PspGI	CCWGG	3 cut(s)	439, 739, 952
PspN4I	GGNNCC	3 cut(s)	594, 1344, 1389
PspPI	GGNCC	5 cut(s)	316, 593, 610, 623, 1387
PspPPI	RGGWCCY	1 cut(s)	623
PspXI	VCTCGAGB	1 cut(s)	897
PsuI	RGATCY	2 cut(s)	847, 943
PsyI	GACNNNGTC	1 cut(s)	571
RsaI	GTAC	6 cut(s)	140, 255, 661, 705, 726, 891
RsaNI	GTAC	6 cut(s)	139, 254, 660, 704, 725, 890
RseI	CAYNNNNRTG	3 cut(s)	796, 1011, 1440
SalI	GTCGAC	1 cut(s)	1156
SapI	GCTCTTC	2 cut(s)	808, 1440
SaqAI	TTAA	8 cut(s)	275, 309, 543, 639, 668, 750, 831, 1515
SatI	GCNGC	2 cut(s)	437, 905
Sau3AI	GATC	7 cut(s)	6, 736, 847, 943, 1282, 1420, 1525
Sau96I	GGNCC	5 cut(s)	316, 593, 610, 623, 1387
ScaI	AGTACT	2 cut(s)	255, 661
SchI	GAGTC	2 cut(s)	42, 1163
ScrFI	CCNGG	4 cut(s)	113, 441, 741, 954
SduI	GDGCHC	2 cut(s)	774, 1452
SfaNI	GCATC	1 cut(s)	21
Sfr274I	CTCGAG	1 cut(s)	897
SfuI	TTCGAA	1 cut(s)	215
SinI	GGWCC	3 cut(s)	593, 623, 1387
SlaI	CTCGAG	1 cut(s)	897
SmiMI	CAYNNNNRTG	3 cut(s)	796, 1011, 1440
SmlI	CTYRAG	3 cut(s)	160, 897, 1042
SmoI	CTYRAG	3 cut(s)	160, 897, 1042
SsiI	CCGC	1 cut(s)	1531
SspI	AATATT	1 cut(s)	1197
SspMI	CTAG	8 cut(s)	102, 131, 257, 296, 353, 576, 627, 1053
StyD4I	CCNGG	4 cut(s)	111, 439, 739, 952
StyI	CCWWGG	2 cut(s)	777, 947
TaaI	ACNGT	2 cut(s)	186, 1418
TaiI	ACGT	5 cut(s)	144, 209, 383, 1085, 1181
TaqI	TCGA	9 cut(s)	20, 36, 42, 193, 215, 855, 898, 1157, 1524
TatI	WGTACW	4 cut(s)	253, 659, 724, 889
TfiI	GAWTC	8 cut(s)	75, 156, 269, 694, 764, 812, 1166, 1240
Tru1I	TTAA	8 cut(s)	275, 309, 543, 639, 668, 750, 831, 1515
Tru9I	TTAA	8 cut(s)	275, 309, 543, 639, 668, 750, 831, 1515
TscAI	CASTG	1 cut(s)	620
TseI	GCWGC	2 cut(s)	436, 904
TspDTI	ATGAA	8 cut(s)	17, 813, 987, 1232, 1258, 1367, 1424, 1458
TspGWI	ACGGA	3 cut(s)	692, 857, 1267
TspRI	CASTG	1 cut(s)	620
Tth111I	GACNNNGTC	1 cut(s)	571
VpaK11BI	GGWCC	3 cut(s)	593, 623, 1387
VspI	ATTAAT	2 cut(s)	831, 1515
XagI	CCTNNNNNAGG	1 cut(s)	695
XbaI	TCTAGA	1 cut(s)	575
XceI	RCATGY	1 cut(s)	1016
XhoI	CTCGAG	1 cut(s)	897
XmiI	GTMKAC	1 cut(s)	1157
XspI	CTAG	8 cut(s)	102, 131, 257, 296, 353, 576, 627, 1053
ZrmI	AGTACT	2 cut(s)	255, 661

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.