MD16G1192700.v1.1

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012073
Physical Location & Seq
Reverse (-)
17045607 .. 17049003
3397 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1192700.v1.1.491

Sequence Viewer

Length: 1131 bp
ATGGGAAAGAAAGACCAGCAGCAGAGAGATGAAGAGAGCAAAGAAAATGGAAGAGTGGAAGCTGTTCTTCAGCTTCTGAGAAAACATGCTCCACTCACAGTCAAACAGGAGAAGTTCTGCAACACTGCTTGTGTTGAGAGGTTTTTGAAAGCAAAAGGAGATAGTGTTAAGAAGGCAGCCAAGCAACTTAGGGCTTGCCTTTCATGGCGACAGAGTGTTGGAACTGAGAACTTGATAGCAGATGAGTTCTCAGCTGAACTCGCTGAAGGCGTTGCCTACACGTCTGGACATGATGAAGAATCGAGACCTGTTGTGATTTTCCGGATTAAACAAGATTACCAGAAGTTCCATTCACAAAAACTGTTCACTCGCTTGTTGGTATTCACGCTGGAGGTGGCGATTGAGTCCATGCTAAAAAACGTCGAGCAGTTCGTCCTCCTTTTCGACGCAAGCTTTTTCAGGTCAGCTTCTGCTTTTATGAACTTGTTGGTGGCAGCACTGAAAATTGTGGCAGAGTACTACCCAGGACGGCTCTTCAAGGCGTTTGTAATTGACCCCCCTACGATGTTCTCGTATCTTTGGAAGGGTGTTCGACCGTTCGTTGAGCTTTCAACGGCTACGATGATGGTATCGTCCCTAGACTTTGAGGAGTCGTTGGATTTCAGTGCCTTCTCAAACTACCCGCGAGCCTCGTCCCTTCGATTCGACCCCTCCTCAATCAAATCAACGGCCAATATCGGCTCCTGCTCATCCTCCCGCTTCTCCTTCACTGTCGCTCAGCATCAGTTCGACTCTCTCAAGCCTTGGTACCTCAGCCTCACAGACACGTCAGCATCCAAAGTAGGCCCCACCACACCCTCCCCGCTGGGACCCGCGCTCATCTCTCCTCTAAACGCCCGGTCCTTCTCCTTCGCATCCCCCGCCGCCAGAACGCCACGTGGAACAATCCACGGCTACGGCAACTCCGCTTCCACGAGAAAGAGCCTGTTCCCGTCGACGCCACTCCCCCAACGGTGCTCAGGCATAGAGGCCAGCAGAACCCCTCATCACCGCCAGCTTCCGCGGACCCCACGTCCCTCGTTCCTCCAATCGCCGGCCACTTCGCAGGTGAGCCACGTTAGCGGGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

377

Amino Acids

41.77

Weight (kDa)

9.56

Isoelectric Point (pI)

48.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 89 - 206 4.6e-19 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012073)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05370 AT3G22410
fragaria_vesca FvH4_4g15420 FvH4_4g15420
malus_domestica MD13G1192100.v1.1 MD16G1192700.v1.1
prunus_persica Prupe.1G012800_v2.0.a1
pyrus_communis pycom13g16480 pycom16g16260
rosa_chinensis RchiOBHm_Chr4g0416361
rosa_laevigata RLG00000007989
rosa_multiflora Rmu_sc0001488.1_g000005
rosa_roxburghii Rroxscaffold_5G00360210
rosa_rugosa Rorug04G0141300
rosa_samantha Rh4AG203700 Rh4BG200900 Rh4CG214900 Rh4DG200600
rosa_wichuraiana Rw4G017200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1096
Acc36I ACCTGC 1 cut(s) 1096
Acc65I GGTACC 1 cut(s) 807
AccB1I GGYRCC 1 cut(s) 807
AccI GTMKAC 1 cut(s) 995
AccII CGCG 3 cut(s) 685, 875, 1063
AccIII TCCGGA 1 cut(s) 321
AcoI YGGCCR 2 cut(s) 729, 1095
AcuI CTGAAG 2 cut(s) 53, 285
AcvI CACGTG 1 cut(s) 938
AcyI GRCGYC 1 cut(s) 998
AfaI GTAC 2 cut(s) 518, 809
AfiI CCNNNNNNNGG 1 cut(s) 1093
AflIII ACRYGT 2 cut(s) 279, 825
AgsI TTSAA 3 cut(s) 148, 538, 612
AhdI GACNNNNNGTC 1 cut(s) 1071
AjiI CACGTC 4 cut(s) 282, 828, 1073, 1128
AjnI CCWGG 1 cut(s) 523
AluBI AGCT 7 cut(s) 62, 73, 254, 453, 467, 607, 1057
AluI AGCT 7 cut(s) 62, 73, 254, 453, 467, 607, 1057
Alw21I GWGCWC 1 cut(s) 1019
Alw26I GTCTC 1 cut(s) 298
AlwNI CAGNNNCTG 2 cut(s) 76, 470
Aor13HI TCCGGA 1 cut(s) 321
AoxI GGCC 4 cut(s) 729, 844, 1029, 1095
ApeKI GCWGC 3 cut(s) 19, 176, 494
Asp700I GAANNNNTTC 1 cut(s) 63
Asp718I GGTACC 1 cut(s) 807
AspLEI GCGC 1 cut(s) 877
AspS9I GGNCC 4 cut(s) 845, 869, 900, 1065
AsuC2I CCSGG 1 cut(s) 898
AsuHPI GGTGA 2 cut(s) 1040, 1120
AvaII GGWCC 3 cut(s) 869, 900, 1065
BanI GGYRCC 1 cut(s) 807
BauI CACGAG 1 cut(s) 973
BbrPI CACGTG 1 cut(s) 938
Bbv12I GWGCWC 1 cut(s) 1019
BbvCI CCTCAGC 1 cut(s) 812
BbvI GCAGC 3 cut(s) 31, 188, 506
BccI CCATC 1 cut(s) 619
BceAI ACGGC 5 cut(s) 545, 630, 744, 967, 973
BciT130I CCWGG 1 cut(s) 525
BcnI CCSGG 1 cut(s) 898
BcoDI GTCTC 1 cut(s) 298
BfaI CTAG 1 cut(s) 638
BfuAI ACCTGC 1 cut(s) 1096
BisI GCNGC 4 cut(s) 20, 177, 495, 924
BlpI GCTNAGC 1 cut(s) 777
BlsI GCNGC 4 cut(s) 21, 178, 496, 925
BmcAI AGTACT 1 cut(s) 518
Bme1390I CCNGG 2 cut(s) 525, 898
Bme18I GGWCC 3 cut(s) 869, 900, 1065
BmeRI GACNNNNNGTC 1 cut(s) 1071
BmgBI CACGTC 4 cut(s) 282, 828, 1073, 1128
BmgT120I GGNCC 4 cut(s) 845, 869, 900, 1065
BmiI GGNNCC 6 cut(s) 742, 809, 847, 870, 871, 1067
BmrFI CCNGG 2 cut(s) 525, 898
BmsI GCATC 3 cut(s) 790, 842, 923
BpmI CTGGAG 1 cut(s) 410
Bpu10I CCTNAGC 2 cut(s) 812, 1018
Bpu1102I GCTNAGC 1 cut(s) 777
BpuEI CTTGAG 1 cut(s) 782
BpuMI CCSGG 1 cut(s) 898
BsaAI YACGTR 1 cut(s) 938
BsaHI GRCGYC 1 cut(s) 998
BsaI GGTCTC 1 cut(s) 298
BsaJI CCNNGG 4 cut(s) 523, 803, 949, 1061
BsaWI WCCGGW 1 cut(s) 321
BsaXI ACNNNNNCTCC 4 cut(s) 150, 180, 947, 977
Bsc4I CCNNNNNNNGG 1 cut(s) 1093
Bse118I RCCGGY 1 cut(s) 1093
BseAI TCCGGA 1 cut(s) 321
BseBI CCWGG 1 cut(s) 525
BseDI CCNNGG 4 cut(s) 523, 803, 949, 1061
BseGI GGATG 3 cut(s) 749, 833, 914
BseLI CCNNNNNNNGG 1 cut(s) 1093
BseMII CTCAG 6 cut(s) 68, 216, 264, 791, 826, 1032
BseRI GAGGAG 3 cut(s) 662, 703, 876
BseXI GCAGC 3 cut(s) 31, 188, 506
BseYI CCCAGC 1 cut(s) 865
Bsh1236I CGCG 3 cut(s) 685, 875, 1063
Bsh1285I CGRYCG 1 cut(s) 596
BshFI GGCC 4 cut(s) 731, 846, 1031, 1097
BshNI GGYRCC 1 cut(s) 807
BsiEI CGRYCG 1 cut(s) 596
BsiHKAI GWGCWC 1 cut(s) 1019
BsiSI CCGG 3 cut(s) 322, 898, 1094
BslFI GGGAC 4 cut(s) 619, 679, 882, 1059
BslI CCNNNNNNNGG 1 cut(s) 1093
BsmAI GTCTC 1 cut(s) 298
BsmFI GGGAC 4 cut(s) 619, 679, 882, 1059
BsnI GGCC 4 cut(s) 731, 846, 1031, 1097
Bso31I GGTCTC 1 cut(s) 298
Bsp1286I GDGCHC 1 cut(s) 1019
Bsp13I TCCGGA 1 cut(s) 321
Bsp1720I GCTNAGC 1 cut(s) 777
BspANI GGCC 4 cut(s) 731, 846, 1031, 1097
BspCNI CTCAG 6 cut(s) 69, 217, 263, 790, 825, 1031
BspEI TCCGGA 1 cut(s) 321
BspFNI CGCG 3 cut(s) 685, 875, 1063
BspLI GGNNCC 6 cut(s) 742, 809, 847, 870, 871, 1067
BspMI ACCTGC 1 cut(s) 1096
BspQI GCTCTTC 1 cut(s) 539
BspT107I GGYRCC 1 cut(s) 807
BspTNI GGTCTC 1 cut(s) 298
BsrFI RCCGGY 1 cut(s) 1093
BssAI RCCGGY 1 cut(s) 1093
BssECI CCNNGG 4 cut(s) 523, 803, 949, 1061
BssNI GRCGYC 1 cut(s) 998
BssSI CACGAG 1 cut(s) 973
BssT1I CCWWGG 1 cut(s) 803
Bst2BI CACGAG 1 cut(s) 973
Bst2UI CCWGG 1 cut(s) 525
Bst4CI ACNGT 5 cut(s) 100, 363, 597, 772, 1014
Bst6I CTCTTC 3 cut(s) 27, 46, 539
BstACI GRCGYC 1 cut(s) 998
BstBAI YACGTR 1 cut(s) 938
BstC8I GCNNGC 6 cut(s) 196, 451, 687, 1033, 1055, 1095
BstDEI CTNAG 7 cut(s) 77, 188, 225, 250, 777, 812, 1018
BstDSI CCRYGG 2 cut(s) 949, 1061
BstF5I GGATG 3 cut(s) 749, 833, 914
BstFNI CGCG 3 cut(s) 685, 875, 1063
BstHHI GCGC 1 cut(s) 877
BstMAI GTCTC 1 cut(s) 298
BstMCI CGRYCG 1 cut(s) 596
BstMWI GCNNNNNNNGC 3 cut(s) 260, 920, 1119
BstNI CCWGG 1 cut(s) 525
BstNSI RCATGY 1 cut(s) 89
BstSCI CCNGG 2 cut(s) 523, 896
BstUI CGCG 3 cut(s) 685, 875, 1063
BstV1I GCAGC 3 cut(s) 31, 188, 506
BsuRI GGCC 4 cut(s) 731, 846, 1031, 1097
BtgI CCRYGG 2 cut(s) 949, 1061
BtrI CACGTC 4 cut(s) 282, 828, 1073, 1128
BtsCI GGATG 3 cut(s) 749, 833, 914
BtsI GCAGTG 1 cut(s) 123
BtsIMutI CAGTG 4 cut(s) 123, 497, 670, 768
BveI ACCTGC 1 cut(s) 1096
Cac8I GCNNGC 6 cut(s) 196, 451, 687, 1033, 1055, 1095
CaiI CAGNNNCTG 2 cut(s) 76, 470
CfoI GCGC 1 cut(s) 877
Cfr10I RCCGGY 1 cut(s) 1093
Cfr13I GGNCC 4 cut(s) 845, 869, 900, 1065
Cfr42I CCGCGG 1 cut(s) 1064
CseI GACGC 2 cut(s) 455, 1006
Csp6I GTAC 2 cut(s) 517, 808
CviAII CATG 4 cut(s) 86, 204, 290, 409
CviQI GTAC 2 cut(s) 517, 808
DdeI CTNAG 7 cut(s) 77, 188, 225, 250, 777, 812, 1018
DriI GACNNNNNGTC 1 cut(s) 1071
EaeI YGGCCR 2 cut(s) 729, 1095
Eam1104I CTCTTC 3 cut(s) 27, 46, 539
Eam1105I GACNNNNNGTC 1 cut(s) 1071
EarI CTCTTC 3 cut(s) 27, 46, 539
Eco130I CCWWGG 1 cut(s) 803
Eco31I GGTCTC 1 cut(s) 298
Eco47I GGWCC 3 cut(s) 869, 900, 1065
Eco57I CTGAAG 2 cut(s) 53, 285
Eco72I CACGTG 1 cut(s) 938
EcoO109I RGGNCCY 2 cut(s) 845, 869
EcoRII CCWGG 1 cut(s) 523
EcoT14I CCWWGG 1 cut(s) 803
ErhI CCWWGG 1 cut(s) 803
FaeI CATG 4 cut(s) 89, 207, 293, 412
FaiI YATR 6 cut(s) 87, 205, 291, 410, 479, 1025
FalI AAGNNNNNCTT 2 cut(s) 51, 83
FaqI GGGAC 4 cut(s) 619, 679, 882, 1059
FatI CATG 4 cut(s) 85, 203, 289, 408
FauI CCCGC 6 cut(s) 690, 764, 870, 880, 928, 1115
FblI GTMKAC 1 cut(s) 995
Fnu4HI GCNGC 4 cut(s) 20, 177, 495, 924
FokI GGATG 3 cut(s) 736, 820, 901
Fsp4HI GCNGC 4 cut(s) 20, 177, 495, 924
FspBI CTAG 1 cut(s) 638
GlaI GCGC 1 cut(s) 876
GluI GCNGC 4 cut(s) 20, 177, 495, 924
GsaI CCCAGC 1 cut(s) 869
GsuI CTGGAG 1 cut(s) 410
HaeIII GGCC 4 cut(s) 731, 846, 1031, 1097
HapII CCGG 3 cut(s) 322, 898, 1094
HgaI GACGC 2 cut(s) 455, 1006
HhaI GCGC 1 cut(s) 877
Hin1I GRCGYC 1 cut(s) 998
Hin1II CATG 4 cut(s) 89, 207, 293, 412
Hin6I GCGC 1 cut(s) 875
HinP1I GCGC 1 cut(s) 875
HincII GTYRAC 1 cut(s) 996
HindII GTYRAC 1 cut(s) 996
HindIII AAGCTT 1 cut(s) 451
HinfI GANTC 5 cut(s) 299, 404, 650, 702, 791
HpaII CCGG 3 cut(s) 322, 898, 1094
HphI GGTGA 2 cut(s) 1040, 1120
Hpy166II GTNNAC 2 cut(s) 366, 996
Hpy188I TCNGA 1 cut(s) 78
Hpy188III TCNNGA 3 cut(s) 285, 303, 322
Hpy8I GTNNAC 2 cut(s) 366, 996
Hpy99I CGWCG 4 cut(s) 425, 449, 997, 1000
HpyAV CCTTC 8 cut(s) 166, 260, 577, 679, 707, 775, 913, 919
HpyCH4III ACNGT 5 cut(s) 100, 363, 597, 772, 1014
HpyCH4IV ACGT 7 cut(s) 281, 420, 827, 937, 1072, 1116, 1127
HpyCH4V TGCA 1 cut(s) 120
HpyF10VI GCNNNNNNNGC 3 cut(s) 260, 920, 1119
HpyF3I CTNAG 7 cut(s) 77, 188, 225, 250, 777, 812, 1018
HpySE526I ACGT 7 cut(s) 281, 420, 827, 937, 1072, 1116, 1127
Hsp92I GRCGYC 1 cut(s) 998
Hsp92II CATG 4 cut(s) 89, 207, 293, 412
HspAI GCGC 1 cut(s) 875
KflI GGGWCCC 1 cut(s) 869
Kpn2I TCCGGA 1 cut(s) 321
KpnI GGTACC 1 cut(s) 811
KroI GCCGGC 1 cut(s) 1093
KroNI GCCGGC 1 cut(s) 1095
KspI CCGCGG 1 cut(s) 1064
LguI GCTCTTC 1 cut(s) 539
LmnI GCTCC 2 cut(s) 94, 746
Lsp1109I GCAGC 3 cut(s) 31, 188, 506
LweI GCATC 3 cut(s) 790, 842, 923
MaeI CTAG 1 cut(s) 638
MaeII ACGT 7 cut(s) 281, 420, 827, 937, 1072, 1116, 1127
MboII GAAGA 5 cut(s) 44, 59, 63, 308, 526
MhlI GDGCHC 1 cut(s) 1019
MluCI AATT 2 cut(s) 504, 549
MlyI GAGTC 3 cut(s) 413, 659, 785
MmeI TCCRAC 2 cut(s) 199, 636
MroI TCCGGA 1 cut(s) 321
MroNI GCCGGC 1 cut(s) 1093
MroXI GAANNNNTTC 1 cut(s) 63
MseI TTAA 2 cut(s) 168, 327
MspA1I CMGCKG 3 cut(s) 254, 865, 1063
MspI CCGG 3 cut(s) 322, 898, 1094
MspR9I CCNGG 2 cut(s) 525, 898
MvaI CCWGG 1 cut(s) 525
MvnI CGCG 3 cut(s) 685, 875, 1063
MwoI GCNNNNNNNGC 3 cut(s) 260, 920, 1119
NaeI GCCGGC 1 cut(s) 1095
NciI CCSGG 1 cut(s) 898
NgoMIV GCCGGC 1 cut(s) 1093
NlaIII CATG 4 cut(s) 89, 207, 293, 412
NlaIV GGNNCC 6 cut(s) 742, 809, 847, 870, 871, 1067
NspI RCATGY 1 cut(s) 89
PaqCI CACCTGC 1 cut(s) 1096
PciSI GCTCTTC 1 cut(s) 539
PcsI WCGNNNNNNNCGW 3 cut(s) 267, 429, 569
PdiI GCCGGC 1 cut(s) 1095
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 2 cut(s) 299, 702
PkrI GCNGC 4 cut(s) 21, 178, 496, 925
PleI GAGTC 3 cut(s) 412, 658, 785
PmaCI CACGTG 1 cut(s) 938
PmlI CACGTG 1 cut(s) 938
PpsI GAGTC 3 cut(s) 412, 658, 785
Ppu21I YACGTR 1 cut(s) 938
PpuMI RGGWCCY 1 cut(s) 869
Psp5II RGGWCCY 1 cut(s) 869
Psp6I CCWGG 1 cut(s) 523
PspCI CACGTG 1 cut(s) 938
PspFI CCCAGC 1 cut(s) 865
PspGI CCWGG 1 cut(s) 523
PspN4I GGNNCC 6 cut(s) 742, 809, 847, 870, 871, 1067
PspPI GGNCC 4 cut(s) 845, 869, 900, 1065
PspPPI RGGWCCY 1 cut(s) 869
PstNI CAGNNNCTG 2 cut(s) 76, 470
PvuII CAGCTG 1 cut(s) 254
RsaI GTAC 2 cut(s) 518, 809
RsaNI GTAC 2 cut(s) 517, 808
SacII CCGCGG 1 cut(s) 1064
SalI GTCGAC 1 cut(s) 994
SapI GCTCTTC 1 cut(s) 539
SaqAI TTAA 2 cut(s) 168, 327
SatI GCNGC 4 cut(s) 20, 177, 495, 924
Sau96I GGNCC 4 cut(s) 845, 869, 900, 1065
ScaI AGTACT 1 cut(s) 518
SchI GAGTC 3 cut(s) 413, 659, 785
ScrFI CCNGG 2 cut(s) 525, 898
SduI GDGCHC 1 cut(s) 1019
SfaNI GCATC 3 cut(s) 790, 842, 923
Sfr303I CCGCGG 1 cut(s) 1064
SgrBI CCGCGG 1 cut(s) 1064
SgrDI CGTCGACG 1 cut(s) 994
SinI GGWCC 3 cut(s) 869, 900, 1065
SmlI CTYRAG 1 cut(s) 797
SmoI CTYRAG 1 cut(s) 797
Sse9I AATT 2 cut(s) 504, 549
SspMI CTAG 1 cut(s) 638
StyD4I CCNGG 2 cut(s) 523, 896
StyI CCWWGG 1 cut(s) 803
TaaI ACNGT 5 cut(s) 100, 363, 597, 772, 1014
TaiI ACGT 7 cut(s) 284, 423, 830, 940, 1075, 1119, 1130
TaqI TCGA 8 cut(s) 302, 423, 444, 592, 700, 705, 789, 995
TasI AATT 2 cut(s) 504, 549
TatI WGTACW 1 cut(s) 516
TauI GCSGC 1 cut(s) 926
TfiI GAWTC 2 cut(s) 299, 702
Tru1I TTAA 2 cut(s) 168, 327
Tru9I TTAA 2 cut(s) 168, 327
TscAI CASTG 4 cut(s) 130, 504, 670, 775
TseI GCWGC 3 cut(s) 19, 176, 494
TspDTI ATGAA 4 cut(s) 45, 192, 309, 494
TspRI CASTG 4 cut(s) 130, 504, 670, 775
VpaK11BI GGWCC 3 cut(s) 869, 900, 1065
XceI RCATGY 1 cut(s) 89
XmiI GTMKAC 1 cut(s) 995
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 1 cut(s) 638
ZrmI AGTACT 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.