Rorug04G0141300

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0012073
Physical Location & Seq
Forward (+)
23345122 .. 23345580
459 bp
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UTR
Exon/CDS
Intron
Rorug04G0141300.1

Sequence Viewer

Length: 459 bp
ATGGGCTACGCTCATTCTACCGCCAAAGGTACTAATTCCAACCAAAGGAATGACATGCAGACACACCGCCAGGCGGGCTACAGCCCCAACGTCGGACCACCTCCAGATCGCCGCCAACGCGCCGCGCCAAGATGGCATCAGAAGCTTCTGAAACTGGGAACTGAGGCATATCAGTCCCACATCGAAAACAAAGAGAAGATCAACCTCTTCCTCACCTATAAAAGGTCCTCTCCTCTCTCCTCATTAATTACGCATTCAATACTTACCTACTGTTACTTTGTCGACATAAATACATTGACTAACTTAGGCATCGGAGAGTTGAAGACCGCCCAACGCGGTCTCCCTCTGACGCCCTTTGTATTTTACTTGACAGGTAGCGGAGGCTTTGAGAACGTCACAAGTATAGATCCGCCCACCGGATCAGCGTTAACAAAGGTTCGGCTACCGCCGAACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

152

Amino Acids

16.93

Weight (kDa)

9.83

Isoelectric Point (pI)

45.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012073)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05370 AT3G22410
fragaria_vesca FvH4_4g15420 FvH4_4g15420
malus_domestica MD13G1192100.v1.1 MD16G1192700.v1.1
prunus_persica Prupe.1G012800_v2.0.a1
pyrus_communis pycom13g16480 pycom16g16260
rosa_chinensis RchiOBHm_Chr4g0416361
rosa_laevigata RLG00000007989
rosa_multiflora Rmu_sc0001488.1_g000005
rosa_roxburghii Rroxscaffold_5G00360210
rosa_rugosa Rorug04G0141300
rosa_samantha Rh4AG203700 Rh4BG200900 Rh4CG214900 Rh4DG200600
rosa_wichuraiana Rw4G017200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 282
AccII CGCG 3 cut(s) 120, 125, 336
AclWI GGATC 2 cut(s) 401, 427
AcyI GRCGYC 1 cut(s) 350
AfaI GTAC 1 cut(s) 31
AfiI CCNNNNNNNGG 5 cut(s) 45, 73, 92, 222, 416
AgsI TTSAA 2 cut(s) 258, 322
AjnI CCWGG 1 cut(s) 69
AluBI AGCT 1 cut(s) 145
AluI AGCT 1 cut(s) 145
Alw26I GTCTC 1 cut(s) 344
AlwI GGATC 2 cut(s) 401, 427
AseI ATTAAT 1 cut(s) 245
AspLEI GCGC 2 cut(s) 122, 127
AspS9I GGNCC 2 cut(s) 95, 225
AsuHPI GGTGA 1 cut(s) 205
AvaII GGWCC 2 cut(s) 95, 225
BbsI GAAGAC 1 cut(s) 329
BccI CCATC 1 cut(s) 126
BciT130I CCWGG 1 cut(s) 71
BcoDI GTCTC 1 cut(s) 344
BfmI CTRYAG 1 cut(s) 79
BglI GCCNNNNNGGC 2 cut(s) 75, 133
BisI GCNGC 2 cut(s) 112, 123
BlsI GCNGC 2 cut(s) 113, 124
Bme1390I CCNGG 1 cut(s) 71
Bme18I GGWCC 2 cut(s) 95, 225
BmgT120I GGNCC 2 cut(s) 95, 225
BmrFI CCNGG 1 cut(s) 71
BmrI ACTGGG 1 cut(s) 164
BmsI GCATC 2 cut(s) 145, 318
BmuI ACTGGG 1 cut(s) 164
BpiI GAAGAC 1 cut(s) 329
BpmI CTGGAG 1 cut(s) 87
BsaHI GRCGYC 1 cut(s) 350
BsaI GGTCTC 1 cut(s) 344
BsaWI WCCGGW 1 cut(s) 416
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bsc4I CCNNNNNNNGG 5 cut(s) 45, 73, 92, 222, 416
Bse1I ACTGG 1 cut(s) 159
BseBI CCWGG 1 cut(s) 71
BseLI CCNNNNNNNGG 5 cut(s) 45, 73, 92, 222, 416
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 1 cut(s) 159
BseRI GAGGAG 2 cut(s) 222, 229
Bsh1236I CGCG 3 cut(s) 120, 125, 336
BsiSI CCGG 1 cut(s) 417
BslFI GGGAC 1 cut(s) 160
BslI CCNNNNNNNGG 5 cut(s) 45, 73, 92, 222, 416
BsmAI GTCTC 1 cut(s) 344
BsmFI GGGAC 1 cut(s) 160
BsmI GAATGC 1 cut(s) 253
Bso31I GGTCTC 1 cut(s) 344
Bsp143I GATC 4 cut(s) 106, 198, 406, 419
BspCNI CTCAG 1 cut(s) 154
BspFNI CGCG 3 cut(s) 120, 125, 336
BspPI GGATC 2 cut(s) 401, 427
BspTNI GGTCTC 1 cut(s) 344
BsrI ACTGG 1 cut(s) 159
BssMI GATC 4 cut(s) 106, 198, 406, 419
BssNI GRCGYC 1 cut(s) 350
Bst2UI CCWGG 1 cut(s) 71
Bst4CI ACNGT 1 cut(s) 272
Bst6I CTCTTC 1 cut(s) 212
BstACI GRCGYC 1 cut(s) 350
BstC8I GCNNGC 1 cut(s) 76
BstDEI CTNAG 2 cut(s) 162, 304
BstENI CCTNNNNNAGG 1 cut(s) 220
BstFNI CGCG 3 cut(s) 120, 125, 336
BstHHI GCGC 2 cut(s) 122, 127
BstKTI GATC 4 cut(s) 109, 201, 409, 422
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 4 cut(s) 106, 198, 406, 419
BstMWI GCNNNNNNNGC 4 cut(s) 75, 117, 133, 142
BstNI CCWGG 1 cut(s) 71
BstNSI RCATGY 1 cut(s) 58
BstSCI CCNGG 1 cut(s) 69
BstSFI CTRYAG 1 cut(s) 79
BstUI CGCG 3 cut(s) 120, 125, 336
BstV2I GAAGAC 1 cut(s) 329
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
Cac8I GCNNGC 1 cut(s) 76
CfoI GCGC 2 cut(s) 122, 127
Cfr13I GGNCC 2 cut(s) 95, 225
CseI GACGC 1 cut(s) 358
Csp6I GTAC 1 cut(s) 30
CviAII CATG 1 cut(s) 55
CviJI RGCY 6 cut(s) 6, 78, 84, 145, 384, 442
CviKI_1 RGCY 6 cut(s) 6, 78, 84, 145, 384, 442
CviQI GTAC 1 cut(s) 30
DdeI CTNAG 2 cut(s) 162, 304
DpnI GATC 4 cut(s) 108, 200, 408, 421
DpnII GATC 4 cut(s) 106, 198, 406, 419
Eam1104I CTCTTC 1 cut(s) 212
EarI CTCTTC 1 cut(s) 212
EciI GGCGGA 1 cut(s) 399
Eco31I GGTCTC 1 cut(s) 344
Eco47I GGWCC 2 cut(s) 95, 225
EcoNI CCTNNNNNAGG 1 cut(s) 220
EcoO109I RGGNCCY 1 cut(s) 225
EcoRII CCWGG 1 cut(s) 69
FaeI CATG 1 cut(s) 58
FaiI YATR 5 cut(s) 56, 169, 219, 287, 404
FaqI GGGAC 1 cut(s) 160
FatI CATG 1 cut(s) 54
FauI CCCGC 1 cut(s) 67
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 2 cut(s) 112, 123
Fsp4HI GCNGC 2 cut(s) 112, 123
GlaI GCGC 2 cut(s) 121, 126
GluI GCNGC 2 cut(s) 112, 123
GsuI CTGGAG 1 cut(s) 87
HapII CCGG 1 cut(s) 417
HgaI GACGC 1 cut(s) 358
HhaI GCGC 2 cut(s) 122, 127
Hin1I GRCGYC 1 cut(s) 350
Hin1II CATG 1 cut(s) 58
Hin6I GCGC 2 cut(s) 120, 125
HinP1I GCGC 2 cut(s) 120, 125
HincII GTYRAC 2 cut(s) 283, 429
HindII GTYRAC 2 cut(s) 283, 429
HindIII AAGCTT 1 cut(s) 143
HpaI GTTAAC 1 cut(s) 429
HpaII CCGG 1 cut(s) 417
HphI GGTGA 1 cut(s) 205
Hpy166II GTNNAC 2 cut(s) 283, 429
Hpy188I TCNGA 5 cut(s) 95, 141, 150, 314, 348
Hpy188III TCNNGA 1 cut(s) 104
Hpy8I GTNNAC 2 cut(s) 283, 429
Hpy99I CGWCG 1 cut(s) 95
HpyCH4III ACNGT 1 cut(s) 272
HpyCH4IV ACGT 2 cut(s) 90, 393
HpyCH4V TGCA 1 cut(s) 58
HpyF10VI GCNNNNNNNGC 4 cut(s) 75, 117, 133, 142
HpyF3I CTNAG 2 cut(s) 162, 304
HpySE526I ACGT 2 cut(s) 90, 393
Hsp92I GRCGYC 1 cut(s) 350
Hsp92II CATG 1 cut(s) 58
HspAI GCGC 2 cut(s) 120, 125
KspAI GTTAAC 1 cut(s) 429
Kzo9I GATC 4 cut(s) 106, 198, 406, 419
LpnPI CCDG 6 cut(s) 56, 83, 117, 140, 357, 430
LweI GCATC 2 cut(s) 145, 318
MaeII ACGT 2 cut(s) 90, 393
MaeIII GTNAC 2 cut(s) 272, 394
MalI GATC 4 cut(s) 108, 200, 408, 421
MboI GATC 4 cut(s) 106, 198, 406, 419
MboII GAAGA 3 cut(s) 199, 208, 334
MflI RGATCY 1 cut(s) 406
MluCI AATT 2 cut(s) 34, 246
MmeI TCCRAC 2 cut(s) 63, 73
MnlI CCTC 9 cut(s) 111, 157, 215, 221, 238, 243, 250, 354, 374
MseI TTAA 2 cut(s) 245, 428
MspI CCGG 1 cut(s) 417
MspR9I CCNGG 1 cut(s) 71
MteI GCGCNGCGC 1 cut(s) 123
Mva1269I GAATGC 1 cut(s) 253
MvaI CCWGG 1 cut(s) 71
MvnI CGCG 3 cut(s) 120, 125, 336
MwoI GCNNNNNNNGC 4 cut(s) 75, 117, 133, 142
NdeII GATC 4 cut(s) 106, 198, 406, 419
NlaIII CATG 1 cut(s) 58
NmuCI GTSAC 1 cut(s) 394
NspI RCATGY 1 cut(s) 58
PctI GAATGC 1 cut(s) 253
PkrI GCNGC 2 cut(s) 113, 124
PpuMI RGGWCCY 1 cut(s) 225
PshBI ATTAAT 1 cut(s) 245
Psp5II RGGWCCY 1 cut(s) 225
Psp6I CCWGG 1 cut(s) 69
PspGI CCWGG 1 cut(s) 69
PspPI GGNCC 2 cut(s) 95, 225
PspPPI RGGWCCY 1 cut(s) 225
PsuI RGATCY 1 cut(s) 406
RsaI GTAC 1 cut(s) 31
RsaNI GTAC 1 cut(s) 30
SalI GTCGAC 1 cut(s) 281
SaqAI TTAA 2 cut(s) 245, 428
SatI GCNGC 2 cut(s) 112, 123
Sau3AI GATC 4 cut(s) 106, 198, 406, 419
Sau96I GGNCC 2 cut(s) 95, 225
ScrFI CCNGG 1 cut(s) 71
SfaNI GCATC 2 cut(s) 145, 318
SfcI CTRYAG 1 cut(s) 79
SinI GGWCC 2 cut(s) 95, 225
Sse9I AATT 2 cut(s) 34, 246
StyD4I CCNGG 1 cut(s) 69
TaaI ACNGT 1 cut(s) 272
TaiI ACGT 2 cut(s) 93, 396
TaqI TCGA 2 cut(s) 183, 282
TasI AATT 2 cut(s) 34, 246
TauI GCSGC 2 cut(s) 114, 125
Tru1I TTAA 2 cut(s) 245, 428
Tru9I TTAA 2 cut(s) 245, 428
TseFI GTSAC 1 cut(s) 394
Tsp45I GTSAC 1 cut(s) 394
VpaK11BI GGWCC 2 cut(s) 95, 225
VspI ATTAAT 1 cut(s) 245
XagI CCTNNNNNAGG 1 cut(s) 220
XceI RCATGY 1 cut(s) 58
XmiI GTMKAC 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.