Rroxscaffold_5G00360210

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012073
Physical Location & Seq
Forward (+)
40543418 .. 40549537
6120 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00360210.1

Sequence Viewer

Length: 1554 bp
ATGGGCAAGAAAGATCAGCACCAGCAAATAATAAGGGATCTCATGAGAGAAAACCCCAAAGTCGAAGCCGTTCTTCACCTTCTCAGAAAACAAGCCCCGCTGACGGTAAAACAGGAGAAGTTTTGTAACAGTGCTTGTGTGGAGCGATTTCTGAAAGCGAAAGGGGAGAGTGTGAAGAAGTCGGCCAAGGCACTCAGAGCTTGCCTTTCCTGGAGAAACACTGTTGGGACTGATCACTTGATAGCAGATGAGTTCTCTGCTGAACTTGCAGAAGGCGTTGCCTACGTGTCTGGTCATGATGAAGAATCCAGGCCTGTTGTGATTTTCCGGTTCAAACAAGACTACCAAAAGTTGCATTCCCAGAAACTGTTTGTTATTGGTATTCACGCTGGAGGTGGCCATTCAGACGATGACCAAAAACGTCGAGCAGTTTGTTCTTCTCTTCGACGCAAGTCAGCGTCGGCTTTTATGAACTTACTTCTGCCAACCATGAAAATTGTGGCAGAATACTACCCACGACGGCTCTTCAAGGCCTTTGTCATTGACCCTCCGTCTCTCTTCTCCTATCTCTGGAAGGGCGTTCGACCGTTCGTTGAGCTATCAACGGCTACAATCATGGTTTCCTCCCTAGACTTCGAAGAGTCGATGGATTTCGACGACTTCTCCTCCTATCCTCGCGCCTCGTCCCTCCGATTCGACCCCTCATCAACGGCCAAGATCGGCTCTTGCTCCTCCTCCCGCTTCTCCTTCACGGTCTCCCACCACCAGATCGACTCCCTCAAGCCGTGGTACCTCAGCCTCACCGACACGTCGTCCTCCAAGGTCGGGCCCACGACCCCCTCCCATCTGGGACCCGCCCTCATCTCCCCACTAAACGCCAGGTCGTTCTCCTTCGCCTCCCCCGCCGTAAGGACGCCACGTGGCACCCTCCACGGCTACGGCAATTCCGCCGCCACCCGGAAGAGCCTGTTCCCGTCGACCCCGCTCCCCCCGCGCTGCCGCCCCATGACGTCGTCATCCAGCGACGGCAGCAGGACCCCCATCCCCCCGTCCGCCGCGGACCCCACGGCCGTCGTTCTTCCAGTCCCCGGCGACGTTCTTCCGCAAGGAGTGCCACGTCAGCCGTACGGAGAAGTCGCGCGAGTCGTTCGTCCCGTTCCTCAAGTTCTACCGGCGGCCGTACGACGAGATGATATACCGGTCGAAAATGCGGCCACCGCTCGGCGGGCTTGTCTCCATTGTCTCCCCCCACCTCAGACGCCGCCACGTGTCCATGTCCCAGCGTTCGTGAAGTTGAAACGCGATAATGTCATATCCGGACCGGAGCTTGCTTCAAAAGAAAAAAGTATGGCATCCGCAGTGCTCGATTGCCAAGATTCGCCGACCAAGGCTCCATTGCTTGAAGAGGTTCCCAAGTTGCAGAGATTGGCTGTAAAGAAGTCCTCTCGCATATATGCTAGAACTTGTTTAGCGCTGGAAGGTGATTTTGGATGGGTATTGATTGGACAAAAGATGATTGCTTTTGAAGAGAATTCTTGGCAGGTTGCTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

517

Amino Acids

56.94

Weight (kDa)

9.51

Isoelectric Point (pI)

52.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 91 - 203 1.7e-08 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012073)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05370 AT3G22410
fragaria_vesca FvH4_4g15420 FvH4_4g15420
malus_domestica MD13G1192100.v1.1 MD16G1192700.v1.1
prunus_persica Prupe.1G012800_v2.0.a1
pyrus_communis pycom13g16480 pycom16g16260
rosa_chinensis RchiOBHm_Chr4g0416361
rosa_laevigata RLG00000007989
rosa_multiflora Rmu_sc0001488.1_g000005
rosa_roxburghii Rroxscaffold_5G00360210
rosa_rugosa Rorug04G0141300
rosa_samantha Rh4AG203700 Rh4BG200900 Rh4CG214900 Rh4DG200600
rosa_wichuraiana Rw4G017200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1013
Acc36I ACCTGC 1 cut(s) 1531
Acc65I GGTACC 1 cut(s) 789
AccB1I GGYRCC 2 cut(s) 789, 923
AccBSI CCGCTC 2 cut(s) 985, 1220
AccI GTMKAC 1 cut(s) 977
AccII CGCG 6 cut(s) 678, 994, 1058, 1139, 1141, 1302
AccIII TCCGGA 1 cut(s) 1316
AclWI GGATC 1 cut(s) 45
AcoI YGGCCR 6 cut(s) 183, 397, 711, 1068, 1176, 1212
AcsI RAATTY 1 cut(s) 1531
AcvI CACGTG 2 cut(s) 920, 1268
AcyI GRCGYC 3 cut(s) 914, 1010, 1259
AfaI GTAC 3 cut(s) 791, 1127, 1182
AfeI AGCGCT 1 cut(s) 1473
AfiI CCNNNNNNNGG 8 cut(s) 103, 570, 825, 909, 957, 1088, 1221, 1224
AflIII ACRYGT 3 cut(s) 285, 807, 1267
AgeI ACCGGT 1 cut(s) 1198
AgsI TTSAA 6 cut(s) 334, 529, 1297, 1335, 1403, 1526
AhdI GACNNNNNGTC 2 cut(s) 550, 811
AjiI CACGTC 2 cut(s) 810, 1118
AjnI CCWGG 3 cut(s) 209, 308, 878
AjuI GAANNNNNNNTTGG 2 cut(s) 1470, 1502
AluBI AGCT 3 cut(s) 200, 598, 1327
AluI AGCT 3 cut(s) 200, 598, 1327
Alw21I GWGCWC 1 cut(s) 1365
Alw26I GTCTC 4 cut(s) 558, 760, 1238, 1247
AlwI GGATC 1 cut(s) 45
AlwNI CAGNNNCTG 1 cut(s) 367
Aor13HI TCCGGA 1 cut(s) 1316
Aor51HI AGCGCT 1 cut(s) 1473
AoxI GGCC 9 cut(s) 183, 311, 397, 531, 711, 827, 1068, 1176, 1212
ApaI GGGCCC 1 cut(s) 831
ApeKI GCWGC 2 cut(s) 996, 1029
ApoI RAATTY 1 cut(s) 1531
AsiGI ACCGGT 1 cut(s) 1198
Asp700I GAANNNNTTC 2 cut(s) 69, 1407
Asp718I GGTACC 1 cut(s) 789
AspLEI GCGC 4 cut(s) 680, 996, 1141, 1474
AspS9I GGNCC 6 cut(s) 827, 828, 851, 1035, 1060, 1319
AsuC2I CCSGG 2 cut(s) 958, 1089
AsuHPI GGTGA 3 cut(s) 68, 793, 1493
AsuII TTCGAA 1 cut(s) 636
AvaII GGWCC 4 cut(s) 851, 1035, 1060, 1319
BaeGI GKGCMC 1 cut(s) 831
BalI TGGCCA 1 cut(s) 399
BanI GGYRCC 2 cut(s) 789, 923
BanII GRGCYC 1 cut(s) 831
BbrPI CACGTG 2 cut(s) 920, 1268
Bbv12I GWGCWC 1 cut(s) 1365
BbvCI CCTCAGC 1 cut(s) 794
BbvI GCAGC 2 cut(s) 983, 1041
BccI CCATC 4 cut(s) 640, 852, 1049, 1485
BciT130I CCWGG 3 cut(s) 211, 310, 880
BclI TGATCA 1 cut(s) 232
BcnI CCSGG 2 cut(s) 958, 1089
BcoDI GTCTC 4 cut(s) 558, 760, 1238, 1247
BfaI CTAG 2 cut(s) 629, 1458
BfoI RGCGCY 1 cut(s) 1475
BfuAI ACCTGC 1 cut(s) 1531
BisI GCNGC 8 cut(s) 951, 997, 1000, 1030, 1056, 1176, 1212, 1262
BlsI GCNGC 8 cut(s) 952, 998, 1001, 1031, 1057, 1177, 1213, 1263
Bme1390I CCNGG 5 cut(s) 211, 310, 880, 958, 1089
Bme18I GGWCC 4 cut(s) 851, 1035, 1060, 1319
BmeRI GACNNNNNGTC 2 cut(s) 550, 811
BmgBI CACGTC 2 cut(s) 810, 1118
BmgT120I GGNCC 6 cut(s) 827, 828, 851, 1035, 1060, 1319
BmiI GGNNCC 9 cut(s) 791, 829, 852, 853, 925, 1037, 1062, 1392, 1410
BmrFI CCNGG 5 cut(s) 211, 310, 880, 958, 1089
BmsI GCATC 1 cut(s) 1361
BoxI GACNNNNGTC 1 cut(s) 451
BpmI CTGGAG 2 cut(s) 232, 411
Bpu10I CCTNAGC 1 cut(s) 794
Bpu14I TTCGAA 1 cut(s) 636
BpuEI CTTGAG 2 cut(s) 764, 1146
BpuMI CCSGG 2 cut(s) 958, 1089
BsaAI YACGTR 3 cut(s) 286, 920, 1268
BsaHI GRCGYC 3 cut(s) 914, 1010, 1259
BsaI GGTCTC 1 cut(s) 760
BsaJI CCNNGG 8 cut(s) 186, 785, 819, 931, 1056, 1065, 1087, 1386
BsaWI WCCGGW 4 cut(s) 327, 1198, 1316, 1321
BsaXI ACNNNNNCTCC 6 cut(s) 647, 650, 677, 680, 1375, 1405
Bsc4I CCNNNNNNNGG 8 cut(s) 103, 570, 825, 909, 957, 1088, 1221, 1224
Bse118I RCCGGY 2 cut(s) 1171, 1198
Bse1I ACTGG 1 cut(s) 1082
Bse3DI GCAATG 1 cut(s) 1394
BseAI TCCGGA 1 cut(s) 1316
BseBI CCWGG 3 cut(s) 211, 310, 880
BseDI CCNNGG 8 cut(s) 186, 785, 819, 931, 1056, 1065, 1087, 1386
BseGI GGATG 4 cut(s) 1016, 1041, 1352, 1496
BseLI CCNNNNNNNGG 8 cut(s) 103, 570, 825, 909, 957, 1088, 1221, 1224
BseMI GCAATG 1 cut(s) 1394
BseMII CTCAG 4 cut(s) 97, 208, 808, 1268
BseNI ACTGG 1 cut(s) 1082
BseRI GAGGAG 3 cut(s) 655, 721, 724
BseSI GKGCMC 1 cut(s) 831
BseX3I CGGCCG 2 cut(s) 1068, 1176
BseXI GCAGC 2 cut(s) 983, 1041
BseYI CCCAGC 1 cut(s) 1279
Bsh1236I CGCG 6 cut(s) 678, 994, 1058, 1139, 1141, 1302
Bsh1285I CGRYCG 4 cut(s) 587, 1071, 1179, 1203
BshFI GGCC 9 cut(s) 185, 313, 399, 533, 713, 829, 1070, 1178, 1214
BshNI GGYRCC 2 cut(s) 789, 923
BshTI ACCGGT 1 cut(s) 1198
BsiEI CGRYCG 4 cut(s) 587, 1071, 1179, 1203
BsiHKAI GWGCWC 1 cut(s) 1365
BsiSI CCGG 7 cut(s) 328, 958, 1089, 1172, 1199, 1317, 1322
BsiWI CGTACG 2 cut(s) 1125, 1180
BslFI GGGAC 6 cut(s) 241, 670, 864, 1070, 1137, 1262
BslI CCNNNNNNNGG 8 cut(s) 103, 570, 825, 909, 957, 1088, 1221, 1224
BsmAI GTCTC 4 cut(s) 558, 760, 1238, 1247
BsmBI CGTCTC 1 cut(s) 558
BsmFI GGGAC 6 cut(s) 241, 670, 864, 1070, 1137, 1262
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 9 cut(s) 185, 313, 399, 533, 713, 829, 1070, 1178, 1214
Bso31I GGTCTC 1 cut(s) 760
Bsp119I TTCGAA 1 cut(s) 636
Bsp120I GGGCCC 1 cut(s) 827
Bsp1286I GDGCHC 2 cut(s) 831, 1365
Bsp13I TCCGGA 1 cut(s) 1316
Bsp143I GATC 5 cut(s) 13, 37, 232, 717, 768
BspANI GGCC 9 cut(s) 185, 313, 399, 533, 713, 829, 1070, 1178, 1214
BspCNI CTCAG 4 cut(s) 96, 207, 807, 1267
BspEI TCCGGA 1 cut(s) 1316
BspFNI CGCG 6 cut(s) 678, 994, 1058, 1139, 1141, 1302
BspHI TCATGA 2 cut(s) 42, 295
BspLI GGNNCC 9 cut(s) 791, 829, 852, 853, 925, 1037, 1062, 1392, 1410
BspMI ACCTGC 1 cut(s) 1531
BspPI GGATC 1 cut(s) 45
BspQI GCTCTTC 2 cut(s) 530, 956
BspT104I TTCGAA 1 cut(s) 636
BspT107I GGYRCC 2 cut(s) 789, 923
BspTNI GGTCTC 1 cut(s) 760
BsrBI CCGCTC 2 cut(s) 985, 1220
BsrDI GCAATG 1 cut(s) 1394
BsrFI RCCGGY 2 cut(s) 1171, 1198
BsrI ACTGG 1 cut(s) 1082
BssAI RCCGGY 2 cut(s) 1171, 1198
BssECI CCNNGG 8 cut(s) 186, 785, 819, 931, 1056, 1065, 1087, 1386
BssMI GATC 5 cut(s) 13, 37, 232, 717, 768
BssNI GRCGYC 3 cut(s) 914, 1010, 1259
BssT1I CCWWGG 3 cut(s) 186, 819, 1386
Bst2UI CCWGG 3 cut(s) 211, 310, 880
Bst4CI ACNGT 6 cut(s) 106, 131, 223, 369, 588, 754
Bst6I CTCTTC 7 cut(s) 447, 530, 563, 633, 956, 1398, 1521
BstACI GRCGYC 3 cut(s) 914, 1010, 1259
BstAPI GCANNNNNTGC 1 cut(s) 1111
BstBAI YACGTR 3 cut(s) 286, 920, 1268
BstBI TTCGAA 1 cut(s) 636
BstC8I GCNNGC 3 cut(s) 202, 1227, 1329
BstDEI CTNAG 4 cut(s) 83, 194, 794, 1254
BstDSI CCRYGG 4 cut(s) 785, 931, 1056, 1065
BstF5I GGATG 4 cut(s) 1016, 1041, 1352, 1496
BstFNI CGCG 6 cut(s) 678, 994, 1058, 1139, 1141, 1302
BstH2I RGCGCY 1 cut(s) 1475
BstHHI GCGC 4 cut(s) 680, 996, 1141, 1474
BstKTI GATC 5 cut(s) 16, 40, 235, 720, 771
BstMAI GTCTC 4 cut(s) 558, 760, 1238, 1247
BstMBI GATC 5 cut(s) 13, 37, 232, 717, 768
BstMCI CGRYCG 4 cut(s) 587, 1071, 1179, 1203
BstMWI GCNNNNNNNGC 9 cut(s) 197, 266, 902, 991, 1029, 1111, 1120, 1217, 1226
BstNI CCWGG 3 cut(s) 211, 310, 880
BstPAI GACNNNNGTC 1 cut(s) 451
BstSCI CCNGG 5 cut(s) 209, 308, 878, 956, 1087
BstSLI GKGCMC 1 cut(s) 831
BstUI CGCG 6 cut(s) 678, 994, 1058, 1139, 1141, 1302
BstV1I GCAGC 2 cut(s) 983, 1041
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BstZI CGGCCG 2 cut(s) 1068, 1176
BsuRI GGCC 9 cut(s) 185, 313, 399, 533, 713, 829, 1070, 1178, 1214
BtgI CCRYGG 4 cut(s) 785, 931, 1056, 1065
BtrI CACGTC 2 cut(s) 810, 1118
BtsCI GGATG 4 cut(s) 1016, 1041, 1352, 1496
BtsI GCAGTG 1 cut(s) 1365
BtsIMutI CAGTG 3 cut(s) 136, 219, 1365
BveI ACCTGC 1 cut(s) 1531
Cac8I GCNNGC 3 cut(s) 202, 1227, 1329
CaiI CAGNNNCTG 1 cut(s) 367
CciI TCATGA 2 cut(s) 42, 295
CfoI GCGC 4 cut(s) 680, 996, 1141, 1474
Cfr10I RCCGGY 2 cut(s) 1171, 1198
Cfr13I GGNCC 6 cut(s) 827, 828, 851, 1035, 1060, 1319
Cfr42I CCGCGG 1 cut(s) 1059
CpoI CGGWCCG 1 cut(s) 1319
CseI GACGC 4 cut(s) 447, 456, 922, 1267
Csp6I GTAC 3 cut(s) 790, 1126, 1181
CspAI ACCGGT 1 cut(s) 1198
CspI CGGWCCG 1 cut(s) 1319
CviAII CATG 6 cut(s) 43, 296, 490, 616, 1006, 1274
CviQI GTAC 3 cut(s) 790, 1126, 1181
DdeI CTNAG 4 cut(s) 83, 194, 794, 1254
DpnI GATC 5 cut(s) 15, 39, 234, 719, 770
DpnII GATC 5 cut(s) 13, 37, 232, 717, 768
DriI GACNNNNNGTC 2 cut(s) 550, 811
EaeI YGGCCR 6 cut(s) 183, 397, 711, 1068, 1176, 1212
EagI CGGCCG 2 cut(s) 1068, 1176
Eam1104I CTCTTC 7 cut(s) 447, 530, 563, 633, 956, 1398, 1521
Eam1105I GACNNNNNGTC 2 cut(s) 550, 811
EarI CTCTTC 7 cut(s) 447, 530, 563, 633, 956, 1398, 1521
EciI GGCGGA 2 cut(s) 937, 1042
EclXI CGGCCG 2 cut(s) 1068, 1176
Eco130I CCWWGG 3 cut(s) 186, 819, 1386
Eco147I AGGCCT 2 cut(s) 313, 533
Eco24I GRGCYC 1 cut(s) 831
Eco31I GGTCTC 1 cut(s) 760
Eco47I GGWCC 4 cut(s) 851, 1035, 1060, 1319
Eco47III AGCGCT 1 cut(s) 1473
Eco52I CGGCCG 2 cut(s) 1068, 1176
Eco72I CACGTG 2 cut(s) 920, 1268
EcoO109I RGGNCCY 2 cut(s) 851, 1035
EcoRI GAATTC 1 cut(s) 1531
EcoRII CCWGG 3 cut(s) 209, 308, 878
EcoT14I CCWWGG 3 cut(s) 186, 819, 1386
EcoT38I GRGCYC 1 cut(s) 831
ErhI CCWWGG 3 cut(s) 186, 819, 1386
Esp3I CGTCTC 1 cut(s) 558
FaeI CATG 6 cut(s) 46, 299, 493, 619, 1009, 1277
FalI AAGNNNNNCTT 2 cut(s) 57, 89
FaqI GGGAC 6 cut(s) 241, 670, 864, 1070, 1137, 1262
FatI CATG 6 cut(s) 42, 295, 489, 615, 1005, 1273
FauI CCCGC 7 cut(s) 105, 746, 862, 910, 990, 999, 1218
FbaI TGATCA 1 cut(s) 232
FblI GTMKAC 1 cut(s) 977
Fnu4HI GCNGC 8 cut(s) 951, 997, 1000, 1030, 1056, 1176, 1212, 1262
FokI GGATG 4 cut(s) 1003, 1028, 1339, 1503
FriOI GRGCYC 1 cut(s) 831
Fsp4HI GCNGC 8 cut(s) 951, 997, 1000, 1030, 1056, 1176, 1212, 1262
FspBI CTAG 2 cut(s) 629, 1458
GlaI GCGC 4 cut(s) 679, 995, 1140, 1473
GluI GCNGC 8 cut(s) 951, 997, 1000, 1030, 1056, 1176, 1212, 1262
GsaI CCCAGC 1 cut(s) 1283
GsuI CTGGAG 2 cut(s) 232, 411
HaeII RGCGCY 1 cut(s) 1475
HaeIII GGCC 9 cut(s) 185, 313, 399, 533, 713, 829, 1070, 1178, 1214
HapII CCGG 7 cut(s) 328, 958, 1089, 1172, 1199, 1317, 1322
HgaI GACGC 4 cut(s) 447, 456, 922, 1267
HhaI GCGC 4 cut(s) 680, 996, 1141, 1474
Hin1I GRCGYC 3 cut(s) 914, 1010, 1259
Hin1II CATG 6 cut(s) 46, 299, 493, 619, 1009, 1277
Hin6I GCGC 4 cut(s) 678, 994, 1139, 1472
HinP1I GCGC 4 cut(s) 678, 994, 1139, 1472
HincII GTYRAC 1 cut(s) 978
HindII GTYRAC 1 cut(s) 978
HinfI GANTC 6 cut(s) 305, 641, 693, 773, 1143, 1376
HpaII CCGG 7 cut(s) 328, 958, 1089, 1172, 1199, 1317, 1322
HphI GGTGA 3 cut(s) 68, 793, 1493
Hpy166II GTNNAC 1 cut(s) 978
Hpy188I TCNGA 6 cut(s) 86, 153, 197, 406, 692, 1257
Hpy188III TCNNGA 5 cut(s) 43, 296, 571, 1288, 1317
Hpy8I GTNNAC 1 cut(s) 978
HpyAV CCTTC 6 cut(s) 89, 266, 568, 757, 901, 1472
HpyCH4III ACNGT 6 cut(s) 106, 131, 223, 369, 588, 754
HpyCH4IV ACGT 8 cut(s) 285, 421, 809, 919, 1010, 1095, 1117, 1267
HpyCH4V TGCA 3 cut(s) 269, 355, 1420
HpyF10VI GCNNNNNNNGC 9 cut(s) 197, 266, 902, 991, 1029, 1111, 1120, 1217, 1226
HpyF3I CTNAG 4 cut(s) 83, 194, 794, 1254
HpySE526I ACGT 8 cut(s) 285, 421, 809, 919, 1010, 1095, 1117, 1267
Hsp92I GRCGYC 3 cut(s) 914, 1010, 1259
Hsp92II CATG 6 cut(s) 46, 299, 493, 619, 1009, 1277
HspAI GCGC 4 cut(s) 678, 994, 1139, 1472
KflI GGGWCCC 1 cut(s) 851
Kpn2I TCCGGA 1 cut(s) 1316
KpnI GGTACC 1 cut(s) 793
Ksp22I TGATCA 1 cut(s) 232
KspI CCGCGG 1 cut(s) 1059
Kzo9I GATC 5 cut(s) 13, 37, 232, 717, 768
LguI GCTCTTC 2 cut(s) 530, 956
LmnI GCTCC 5 cut(s) 142, 734, 990, 1324, 1396
Lsp1109I GCAGC 2 cut(s) 983, 1041
LweI GCATC 1 cut(s) 1361
MaeI CTAG 2 cut(s) 629, 1458
MaeII ACGT 8 cut(s) 285, 421, 809, 919, 1010, 1095, 1117, 1267
MaeIII GTNAC 1 cut(s) 125
MalI GATC 5 cut(s) 15, 39, 234, 719, 770
MbiI CCGCTC 2 cut(s) 985, 1220
MboI GATC 5 cut(s) 13, 37, 232, 717, 768
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 2 cut(s) 831, 1365
MlsI TGGCCA 1 cut(s) 399
MluCI AATT 3 cut(s) 495, 943, 1531
MluNI TGGCCA 1 cut(s) 399
MlyI GAGTC 3 cut(s) 650, 767, 1152
Mox20I TGGCCA 1 cut(s) 399
MroI TCCGGA 1 cut(s) 1316
MroXI GAANNNNTTC 2 cut(s) 69, 1407
MscI TGGCCA 1 cut(s) 399
MseI TTAA 1 cut(s) 1552
Msp20I TGGCCA 1 cut(s) 399
MspA1I CMGCKG 2 cut(s) 100, 1058
MspI CCGG 7 cut(s) 328, 958, 1089, 1172, 1199, 1317, 1322
MspR9I CCNGG 5 cut(s) 211, 310, 880, 958, 1089
Mva1269I GAATGC 1 cut(s) 355
MvaI CCWGG 3 cut(s) 211, 310, 880
MvnI CGCG 6 cut(s) 678, 994, 1058, 1139, 1141, 1302
MwoI GCNNNNNNNGC 9 cut(s) 197, 266, 902, 991, 1029, 1111, 1120, 1217, 1226
NciI CCSGG 2 cut(s) 958, 1089
NdeII GATC 5 cut(s) 13, 37, 232, 717, 768
NlaIII CATG 6 cut(s) 46, 299, 493, 619, 1009, 1277
NlaIV GGNNCC 9 cut(s) 791, 829, 852, 853, 925, 1037, 1062, 1392, 1410
NmeAIII GCCGAG 1 cut(s) 1201
NspV TTCGAA 1 cut(s) 636
PagI TCATGA 2 cut(s) 42, 295
PceI AGGCCT 2 cut(s) 313, 533
PciSI GCTCTTC 2 cut(s) 530, 956
PcsI WCGNNNNNNNCGW 2 cut(s) 1143, 1152
PctI GAATGC 1 cut(s) 355
PdmI GAANNNNTTC 2 cut(s) 69, 1407
PfeI GAWTC 3 cut(s) 305, 693, 1376
Pfl23II CGTACG 2 cut(s) 1125, 1180
PflFI GACNNNGTC 1 cut(s) 1012
PfoI TCCNGGA 1 cut(s) 209
PinAI ACCGGT 1 cut(s) 1198
PkrI GCNGC 8 cut(s) 952, 998, 1001, 1031, 1057, 1177, 1213, 1263
PleI GAGTC 3 cut(s) 649, 767, 1151
PmaCI CACGTG 2 cut(s) 920, 1268
PmlI CACGTG 2 cut(s) 920, 1268
PpsI GAGTC 3 cut(s) 649, 767, 1151
Ppu21I YACGTR 3 cut(s) 286, 920, 1268
PpuMI RGGWCCY 2 cut(s) 851, 1035
PshAI GACNNNNGTC 1 cut(s) 451
Psp5II RGGWCCY 2 cut(s) 851, 1035
Psp6I CCWGG 3 cut(s) 209, 308, 878
PspCI CACGTG 2 cut(s) 920, 1268
PspFI CCCAGC 1 cut(s) 1279
PspGI CCWGG 3 cut(s) 209, 308, 878
PspLI CGTACG 2 cut(s) 1125, 1180
PspN4I GGNNCC 9 cut(s) 791, 829, 852, 853, 925, 1037, 1062, 1392, 1410
PspOMI GGGCCC 1 cut(s) 827
PspPI GGNCC 6 cut(s) 827, 828, 851, 1035, 1060, 1319
PspPPI RGGWCCY 2 cut(s) 851, 1035
PstNI CAGNNNCTG 1 cut(s) 367
PsuI RGATCY 1 cut(s) 37
PsyI GACNNNGTC 1 cut(s) 1012
RsaI GTAC 3 cut(s) 791, 1127, 1182
RsaNI GTAC 3 cut(s) 790, 1126, 1181
Rsr2I CGGWCCG 1 cut(s) 1319
RsrII CGGWCCG 1 cut(s) 1319
SacII CCGCGG 1 cut(s) 1059
SalI GTCGAC 1 cut(s) 976
SapI GCTCTTC 2 cut(s) 530, 956
SaqAI TTAA 1 cut(s) 1552
SatI GCNGC 8 cut(s) 951, 997, 1000, 1030, 1056, 1176, 1212, 1262
Sau3AI GATC 5 cut(s) 13, 37, 232, 717, 768
Sau96I GGNCC 6 cut(s) 827, 828, 851, 1035, 1060, 1319
SchI GAGTC 3 cut(s) 650, 767, 1152
ScrFI CCNGG 5 cut(s) 211, 310, 880, 958, 1089
SduI GDGCHC 2 cut(s) 831, 1365
SfaNI GCATC 1 cut(s) 1361
Sfr303I CCGCGG 1 cut(s) 1059
SfuI TTCGAA 1 cut(s) 636
SgrBI CCGCGG 1 cut(s) 1059
SinI GGWCC 4 cut(s) 851, 1035, 1060, 1319
SmlI CTYRAG 2 cut(s) 779, 1161
SmoI CTYRAG 2 cut(s) 779, 1161
Sse9I AATT 3 cut(s) 495, 943, 1531
SseBI AGGCCT 2 cut(s) 313, 533
SspMI CTAG 2 cut(s) 629, 1458
StuI AGGCCT 2 cut(s) 313, 533
StyD4I CCNGG 5 cut(s) 209, 308, 878, 956, 1087
StyI CCWWGG 3 cut(s) 186, 819, 1386
TaaI ACNGT 6 cut(s) 106, 131, 223, 369, 588, 754
TaiI ACGT 8 cut(s) 288, 424, 812, 922, 1013, 1098, 1120, 1270
TasI AATT 3 cut(s) 495, 943, 1531
TauI GCSGC 6 cut(s) 953, 1002, 1058, 1178, 1214, 1264
TfiI GAWTC 3 cut(s) 305, 693, 1376
Tru1I TTAA 1 cut(s) 1552
Tru9I TTAA 1 cut(s) 1552
TscAI CASTG 3 cut(s) 136, 226, 1365
TseI GCWGC 2 cut(s) 996, 1029
TspDTI ATGAA 3 cut(s) 315, 485, 506
TspGWI ACGGA 2 cut(s) 540, 1143
TspRI CASTG 3 cut(s) 136, 226, 1365
Tth111I GACNNNGTC 1 cut(s) 1012
VpaK11BI GGWCC 4 cut(s) 851, 1035, 1060, 1319
XapI RAATTY 1 cut(s) 1531
XcmI CCANNNNNNNNNTGG 1 cut(s) 496
XmiI GTMKAC 1 cut(s) 977
XmnI GAANNNNTTC 2 cut(s) 69, 1407
XspI CTAG 2 cut(s) 629, 1458
ZraI GACGTC 1 cut(s) 1011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.