Rh4AG203700

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0012073
Physical Location & Seq
Forward (+)
48914693 .. 48918994
4302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG203700.1

Sequence Viewer

Length: 1308 bp
ATGGGCAAGAAAGATCAGCACCAGCAAATAATAAGGGATCTCATGAGAGACAACCCCAAAGTCGAAGCCGTTCTTCACCTTCTCAGAAAACAAGCCCCGCTGACGGTAAAACAGGAGAAGTTTTGTAACAGTGCTTGTGTGCAGAGGTTTCTGAAAGCGAAAGGGGAGAGTGTGAAGAAGTCGGCCAAGGCACTCAGAGCTTGCCTTTCCTGGAGAAACACTGTTGGGACTGATCACTTGATAGCAGATGAGTTCTCTGCTGAACTTGCAGAAGGCGTTGCCTACGTTTCTGGTCATGATGAAGAATCCAGGCCTGTTGTGATTTTCCGGTTCAAACAAGACTACCAAAAGTTGCATTCCCAGAAACTGTTCACTAGGTTATTGGTATTCACGCTGGAGGTGGCCATTCAGACGATGACCAAAAACGTCGAGCAGTTTGTTCTTCTCTTCGACGCAAGCTTTTTCAGGTCAGCGTCGGCTTTTATGAACCTGCTTCTGCCAACTATGAAAATTGTGGCAGAGTACTACCCAGGACGGCTCTTCAAGGCCTTTGTCATTGACCCTCCGTCTCTCTTCTCCTATCTCTGGAAGGGCGTTCGACCGTTCGTTGAGCTATCAACGGCTACGATCATGGTTTCCTCCCTAGACTTCGAAGAGTCGATGGATTTCGACCACTTCTCCTCCTATCCTCGCGCCTCGTCCCTCCGATTCGACCCCTCATCAACGGCCAAGATCGGCTCCTGCTCCTCCTCCCGCTTCTCCTTCACCGTCTCCCACCACCAGATCGACTCCCTCAAGCCGTGGTACCTAAGCCTCACCGACACGTCGTCCTCCAAGGTCGGGCCCAACACCCCCTCCCATCTGGGACCCGCCCTCATCTCCCCACTCAACGCCAGGTCGTTCTCCTTCGCCTCCCCCGCCGCAAGGACTCCACGTGGCACCCTCCACGGCTACGGCAACTCCGCCGCCACCCGGAAGAGCCTGTTCCCGTCGACCCCGCTCCCCCAGCGCTGCCGCCCCATGACGTCGTCATCCAGCGACGGCAGCAGGACCCCCCATCCTCCCCGTCCGCCGCGGACCCCACGGCCGTCGTTCTTCCAGTCCCCGGCGACGTTCTTCCGCAAGGAGTGCCACGTCAGCCGGACGGAGAAGTCGCGCGAGTCGTTCGTCCCGTTCCTCAAGTTCTACCGGCGGCCGTACGACGAGATGATATACCGGTCGAAAATGCGGCCACCGCTCGGCGGGCTCGTCTCCATTGTCTCCCCCCACCTCAGACGCCGCCACGTGTCCATGTCCCAGCGGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

435

Amino Acids

49.35

Weight (kDa)

10.05

Isoelectric Point (pI)

53.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 91 - 208 1.2e-20 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012073)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05370 AT3G22410
fragaria_vesca FvH4_4g15420 FvH4_4g15420
malus_domestica MD13G1192100.v1.1 MD16G1192700.v1.1
prunus_persica Prupe.1G012800_v2.0.a1
pyrus_communis pycom13g16480 pycom16g16260
rosa_chinensis RchiOBHm_Chr4g0416361
rosa_laevigata RLG00000007989
rosa_multiflora Rmu_sc0001488.1_g000005
rosa_roxburghii Rroxscaffold_5G00360210
rosa_rugosa Rorug04G0141300
rosa_samantha Rh4AG203700 Rh4BG200900 Rh4CG214900 Rh4DG200600
rosa_wichuraiana Rw4G017200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1150
AatII GACGTC 1 cut(s) 1028
Acc36I ACCTGC 1 cut(s) 498
Acc65I GGTACC 1 cut(s) 804
AccB1I GGYRCC 2 cut(s) 804, 938
AccBSI CCGCTC 2 cut(s) 1000, 1237
AccI GTMKAC 1 cut(s) 992
AccII CGCG 4 cut(s) 693, 1075, 1156, 1158
AclWI GGATC 1 cut(s) 45
AcoI YGGCCR 6 cut(s) 183, 402, 726, 1085, 1193, 1229
AcvI CACGTG 2 cut(s) 935, 1285
AcyI GRCGYC 2 cut(s) 1025, 1276
AfaI GTAC 4 cut(s) 524, 806, 1199, 1304
AfeI AGCGCT 1 cut(s) 1010
AfiI CCNNNNNNNGG 8 cut(s) 103, 585, 840, 924, 972, 1105, 1238, 1241
AflIII ACRYGT 2 cut(s) 822, 1284
AgeI ACCGGT 1 cut(s) 1215
AgsI TTSAA 2 cut(s) 334, 544
AhdI GACNNNNNGTC 2 cut(s) 565, 826
AjiI CACGTC 2 cut(s) 825, 1135
AjnI CCWGG 4 cut(s) 209, 308, 529, 893
AluBI AGCT 3 cut(s) 200, 459, 613
AluI AGCT 3 cut(s) 200, 459, 613
Alw26I GTCTC 5 cut(s) 42, 573, 775, 1255, 1264
AlwI GGATC 1 cut(s) 45
AlwNI CAGNNNCTG 1 cut(s) 367
Aor51HI AGCGCT 1 cut(s) 1010
AoxI GGCC 9 cut(s) 183, 311, 402, 546, 726, 842, 1085, 1193, 1229
ApaI GGGCCC 1 cut(s) 846
ApeKI GCWGC 2 cut(s) 1011, 1044
AsiGI ACCGGT 1 cut(s) 1215
Asp700I GAANNNNTTC 2 cut(s) 69, 368
Asp718I GGTACC 1 cut(s) 804
AspLEI GCGC 3 cut(s) 695, 1011, 1158
AspS9I GGNCC 5 cut(s) 842, 843, 866, 1050, 1077
AsuC2I CCSGG 2 cut(s) 973, 1106
AsuHPI GGTGA 3 cut(s) 68, 757, 808
AsuII TTCGAA 1 cut(s) 651
AvaII GGWCC 3 cut(s) 866, 1050, 1077
BaeGI GKGCMC 1 cut(s) 846
BalI TGGCCA 1 cut(s) 404
BanI GGYRCC 2 cut(s) 804, 938
BanII GRGCYC 2 cut(s) 846, 1248
BbrPI CACGTG 2 cut(s) 935, 1285
BbvI GCAGC 2 cut(s) 998, 1056
BccI CCATC 3 cut(s) 655, 867, 1065
BciT130I CCWGG 4 cut(s) 211, 310, 531, 895
BclI TGATCA 1 cut(s) 232
BcnI CCSGG 2 cut(s) 973, 1106
BcoDI GTCTC 5 cut(s) 42, 573, 775, 1255, 1264
BfaI CTAG 2 cut(s) 375, 644
BfoI RGCGCY 1 cut(s) 1012
BfuAI ACCTGC 1 cut(s) 498
BisI GCNGC 9 cut(s) 921, 966, 1012, 1015, 1045, 1073, 1193, 1229, 1279
BlsI GCNGC 9 cut(s) 922, 967, 1013, 1016, 1046, 1074, 1194, 1230, 1280
BmcAI AGTACT 1 cut(s) 524
Bme1390I CCNGG 6 cut(s) 211, 310, 531, 895, 973, 1106
Bme18I GGWCC 3 cut(s) 866, 1050, 1077
BmeRI GACNNNNNGTC 2 cut(s) 565, 826
BmgBI CACGTC 2 cut(s) 825, 1135
BmgT120I GGNCC 5 cut(s) 842, 843, 866, 1050, 1077
BmiI GGNNCC 8 cut(s) 739, 806, 844, 867, 868, 940, 1052, 1079
BmrFI CCNGG 6 cut(s) 211, 310, 531, 895, 973, 1106
BpmI CTGGAG 2 cut(s) 232, 416
Bpu10I CCTNAGC 1 cut(s) 809
Bpu14I TTCGAA 1 cut(s) 651
BpuEI CTTGAG 2 cut(s) 779, 1163
BpuMI CCSGG 2 cut(s) 973, 1106
BsaAI YACGTR 2 cut(s) 935, 1285
BsaHI GRCGYC 2 cut(s) 1025, 1276
BsaJI CCNNGG 8 cut(s) 186, 529, 800, 834, 946, 1073, 1082, 1104
BsaWI WCCGGW 2 cut(s) 327, 1215
BsaXI ACNNNNNCTCC 8 cut(s) 662, 665, 692, 695, 839, 869, 944, 974
Bsc4I CCNNNNNNNGG 8 cut(s) 103, 585, 840, 924, 972, 1105, 1238, 1241
Bse118I RCCGGY 2 cut(s) 1188, 1215
Bse1I ACTGG 1 cut(s) 1099
BseBI CCWGG 4 cut(s) 211, 310, 531, 895
BseDI CCNNGG 8 cut(s) 186, 529, 800, 834, 946, 1073, 1082, 1104
BseGI GGATG 2 cut(s) 1031, 1057
BseLI CCNNNNNNNGG 8 cut(s) 103, 585, 840, 924, 972, 1105, 1238, 1241
BseMII CTCAG 3 cut(s) 97, 208, 1285
BseNI ACTGG 1 cut(s) 1099
BseRI GAGGAG 3 cut(s) 670, 736, 739
BseSI GKGCMC 1 cut(s) 846
BseX3I CGGCCG 2 cut(s) 1085, 1193
BseXI GCAGC 2 cut(s) 998, 1056
BseYI CCCAGC 2 cut(s) 1005, 1296
BsgI GTGCAG 1 cut(s) 161
Bsh1236I CGCG 4 cut(s) 693, 1075, 1156, 1158
Bsh1285I CGRYCG 4 cut(s) 602, 1088, 1196, 1220
BshFI GGCC 9 cut(s) 185, 313, 404, 548, 728, 844, 1087, 1195, 1231
BshNI GGYRCC 2 cut(s) 804, 938
BshTI ACCGGT 1 cut(s) 1215
BsiEI CGRYCG 4 cut(s) 602, 1088, 1196, 1220
BsiSI CCGG 6 cut(s) 328, 973, 1106, 1141, 1189, 1216
BsiWI CGTACG 1 cut(s) 1197
BslFI GGGAC 6 cut(s) 241, 685, 879, 1087, 1154, 1279
BslI CCNNNNNNNGG 8 cut(s) 103, 585, 840, 924, 972, 1105, 1238, 1241
BsmAI GTCTC 5 cut(s) 42, 573, 775, 1255, 1264
BsmBI CGTCTC 3 cut(s) 573, 775, 1255
BsmFI GGGAC 6 cut(s) 241, 685, 879, 1087, 1154, 1279
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 9 cut(s) 185, 313, 404, 548, 728, 844, 1087, 1195, 1231
Bsp119I TTCGAA 1 cut(s) 651
Bsp120I GGGCCC 1 cut(s) 842
Bsp1286I GDGCHC 2 cut(s) 846, 1248
Bsp143I GATC 6 cut(s) 13, 37, 232, 627, 732, 783
BspANI GGCC 9 cut(s) 185, 313, 404, 548, 728, 844, 1087, 1195, 1231
BspCNI CTCAG 3 cut(s) 96, 207, 1284
BspFNI CGCG 4 cut(s) 693, 1075, 1156, 1158
BspHI TCATGA 2 cut(s) 42, 295
BspLI GGNNCC 8 cut(s) 739, 806, 844, 867, 868, 940, 1052, 1079
BspMI ACCTGC 1 cut(s) 498
BspPI GGATC 1 cut(s) 45
BspQI GCTCTTC 2 cut(s) 545, 971
BspT104I TTCGAA 1 cut(s) 651
BspT107I GGYRCC 2 cut(s) 804, 938
BsrBI CCGCTC 2 cut(s) 1000, 1237
BsrFI RCCGGY 2 cut(s) 1188, 1215
BsrI ACTGG 1 cut(s) 1099
BssAI RCCGGY 2 cut(s) 1188, 1215
BssECI CCNNGG 8 cut(s) 186, 529, 800, 834, 946, 1073, 1082, 1104
BssMI GATC 6 cut(s) 13, 37, 232, 627, 732, 783
BssNI GRCGYC 2 cut(s) 1025, 1276
BssT1I CCWWGG 2 cut(s) 186, 834
Bst2UI CCWGG 4 cut(s) 211, 310, 531, 895
Bst4CI ACNGT 6 cut(s) 106, 131, 223, 369, 603, 769
Bst6I CTCTTC 5 cut(s) 452, 545, 578, 648, 971
BstACI GRCGYC 2 cut(s) 1025, 1276
BstAPI GCANNNNNTGC 1 cut(s) 1128
BstBAI YACGTR 2 cut(s) 935, 1285
BstBI TTCGAA 1 cut(s) 651
BstC8I GCNNGC 3 cut(s) 202, 457, 1244
BstDEI CTNAG 4 cut(s) 83, 194, 809, 1271
BstDSI CCRYGG 4 cut(s) 800, 946, 1073, 1082
BstF5I GGATG 2 cut(s) 1031, 1057
BstFNI CGCG 4 cut(s) 693, 1075, 1156, 1158
BstH2I RGCGCY 1 cut(s) 1012
BstHHI GCGC 3 cut(s) 695, 1011, 1158
BstKTI GATC 6 cut(s) 16, 40, 235, 630, 735, 786
BstMAI GTCTC 5 cut(s) 42, 573, 775, 1255, 1264
BstMBI GATC 6 cut(s) 13, 37, 232, 627, 732, 783
BstMCI CGRYCG 4 cut(s) 602, 1088, 1196, 1220
BstMWI GCNNNNNNNGC 9 cut(s) 197, 266, 917, 1006, 1044, 1128, 1137, 1234, 1243
BstNI CCWGG 4 cut(s) 211, 310, 531, 895
BstSCI CCNGG 6 cut(s) 209, 308, 529, 893, 971, 1104
BstSLI GKGCMC 1 cut(s) 846
BstUI CGCG 4 cut(s) 693, 1075, 1156, 1158
BstV1I GCAGC 2 cut(s) 998, 1056
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BstZI CGGCCG 2 cut(s) 1085, 1193
BsuRI GGCC 9 cut(s) 185, 313, 404, 548, 728, 844, 1087, 1195, 1231
BtgI CCRYGG 4 cut(s) 800, 946, 1073, 1082
BtrI CACGTC 2 cut(s) 825, 1135
BtsCI GGATG 2 cut(s) 1031, 1057
BtsIMutI CAGTG 2 cut(s) 136, 219
BveI ACCTGC 1 cut(s) 498
Cac8I GCNNGC 3 cut(s) 202, 457, 1244
CaiI CAGNNNCTG 1 cut(s) 367
CciI TCATGA 2 cut(s) 42, 295
CfoI GCGC 3 cut(s) 695, 1011, 1158
Cfr10I RCCGGY 2 cut(s) 1188, 1215
Cfr13I GGNCC 5 cut(s) 842, 843, 866, 1050, 1077
Cfr42I CCGCGG 1 cut(s) 1076
CseI GACGC 3 cut(s) 461, 462, 1284
Csp6I GTAC 4 cut(s) 523, 805, 1198, 1303
CspAI ACCGGT 1 cut(s) 1215
CviAII CATG 5 cut(s) 43, 296, 631, 1021, 1291
CviQI GTAC 4 cut(s) 523, 805, 1198, 1303
DdeI CTNAG 4 cut(s) 83, 194, 809, 1271
DpnI GATC 6 cut(s) 15, 39, 234, 629, 734, 785
DpnII GATC 6 cut(s) 13, 37, 232, 627, 732, 783
DrdI GACNNNNNNGTC 1 cut(s) 1150
DriI GACNNNNNGTC 2 cut(s) 565, 826
DseDI GACNNNNNNGTC 1 cut(s) 1150
EaeI YGGCCR 6 cut(s) 183, 402, 726, 1085, 1193, 1229
EagI CGGCCG 2 cut(s) 1085, 1193
Eam1104I CTCTTC 5 cut(s) 452, 545, 578, 648, 971
Eam1105I GACNNNNNGTC 2 cut(s) 565, 826
EarI CTCTTC 5 cut(s) 452, 545, 578, 648, 971
EciI GGCGGA 2 cut(s) 952, 1059
EclXI CGGCCG 2 cut(s) 1085, 1193
Eco130I CCWWGG 2 cut(s) 186, 834
Eco147I AGGCCT 2 cut(s) 313, 548
Eco24I GRGCYC 2 cut(s) 846, 1248
Eco47I GGWCC 3 cut(s) 866, 1050, 1077
Eco47III AGCGCT 1 cut(s) 1010
Eco52I CGGCCG 2 cut(s) 1085, 1193
Eco72I CACGTG 2 cut(s) 935, 1285
EcoO109I RGGNCCY 2 cut(s) 866, 1050
EcoRII CCWGG 4 cut(s) 209, 308, 529, 893
EcoT14I CCWWGG 2 cut(s) 186, 834
EcoT38I GRGCYC 2 cut(s) 846, 1248
ErhI CCWWGG 2 cut(s) 186, 834
Esp3I CGTCTC 3 cut(s) 573, 775, 1255
FaeI CATG 5 cut(s) 46, 299, 634, 1024, 1294
FaiI YATR 8 cut(s) 44, 297, 485, 506, 632, 1022, 1213, 1292
FalI AAGNNNNNCTT 2 cut(s) 57, 89
FaqI GGGAC 6 cut(s) 241, 685, 879, 1087, 1154, 1279
FatI CATG 5 cut(s) 42, 295, 630, 1020, 1290
FauI CCCGC 6 cut(s) 105, 761, 877, 925, 1005, 1235
FbaI TGATCA 1 cut(s) 232
FblI GTMKAC 1 cut(s) 992
Fnu4HI GCNGC 9 cut(s) 921, 966, 1012, 1015, 1045, 1073, 1193, 1229, 1279
FokI GGATG 2 cut(s) 1018, 1044
FriOI GRGCYC 2 cut(s) 846, 1248
Fsp4HI GCNGC 9 cut(s) 921, 966, 1012, 1015, 1045, 1073, 1193, 1229, 1279
FspBI CTAG 2 cut(s) 375, 644
GlaI GCGC 3 cut(s) 694, 1010, 1157
GluI GCNGC 9 cut(s) 921, 966, 1012, 1015, 1045, 1073, 1193, 1229, 1279
GsaI CCCAGC 2 cut(s) 1009, 1300
GsuI CTGGAG 2 cut(s) 232, 416
HaeII RGCGCY 1 cut(s) 1012
HaeIII GGCC 9 cut(s) 185, 313, 404, 548, 728, 844, 1087, 1195, 1231
HapII CCGG 6 cut(s) 328, 973, 1106, 1141, 1189, 1216
HgaI GACGC 3 cut(s) 461, 462, 1284
HhaI GCGC 3 cut(s) 695, 1011, 1158
Hin1I GRCGYC 2 cut(s) 1025, 1276
Hin1II CATG 5 cut(s) 46, 299, 634, 1024, 1294
Hin6I GCGC 3 cut(s) 693, 1009, 1156
HinP1I GCGC 3 cut(s) 693, 1009, 1156
HincII GTYRAC 1 cut(s) 993
HindII GTYRAC 1 cut(s) 993
HindIII AAGCTT 1 cut(s) 457
HinfI GANTC 6 cut(s) 305, 656, 708, 788, 928, 1160
HpaII CCGG 6 cut(s) 328, 973, 1106, 1141, 1189, 1216
HphI GGTGA 3 cut(s) 68, 757, 808
Hpy166II GTNNAC 2 cut(s) 372, 993
Hpy188I TCNGA 6 cut(s) 86, 153, 197, 411, 707, 1274
Hpy188III TCNNGA 3 cut(s) 43, 296, 586
Hpy8I GTNNAC 2 cut(s) 372, 993
HpyAV CCTTC 5 cut(s) 89, 266, 583, 772, 916
HpyCH4III ACNGT 6 cut(s) 106, 131, 223, 369, 603, 769
HpyCH4IV ACGT 8 cut(s) 285, 426, 824, 934, 1025, 1112, 1134, 1284
HpyCH4V TGCA 3 cut(s) 142, 269, 355
HpyF10VI GCNNNNNNNGC 9 cut(s) 197, 266, 917, 1006, 1044, 1128, 1137, 1234, 1243
HpyF3I CTNAG 4 cut(s) 83, 194, 809, 1271
HpySE526I ACGT 8 cut(s) 285, 426, 824, 934, 1025, 1112, 1134, 1284
Hsp92I GRCGYC 2 cut(s) 1025, 1276
Hsp92II CATG 5 cut(s) 46, 299, 634, 1024, 1294
HspAI GCGC 3 cut(s) 693, 1009, 1156
KflI GGGWCCC 1 cut(s) 866
KpnI GGTACC 1 cut(s) 808
Ksp22I TGATCA 1 cut(s) 232
KspI CCGCGG 1 cut(s) 1076
Kzo9I GATC 6 cut(s) 13, 37, 232, 627, 732, 783
LguI GCTCTTC 2 cut(s) 545, 971
LmnI GCTCC 3 cut(s) 743, 749, 1005
Lsp1109I GCAGC 2 cut(s) 998, 1056
MaeI CTAG 2 cut(s) 375, 644
MaeII ACGT 8 cut(s) 285, 426, 824, 934, 1025, 1112, 1134, 1284
MaeIII GTNAC 1 cut(s) 125
MalI GATC 6 cut(s) 15, 39, 234, 629, 734, 785
MbiI CCGCTC 2 cut(s) 1000, 1237
MboI GATC 6 cut(s) 13, 37, 232, 627, 732, 783
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 2 cut(s) 846, 1248
MlsI TGGCCA 1 cut(s) 404
MluCI AATT 1 cut(s) 510
MluNI TGGCCA 1 cut(s) 404
MlyI GAGTC 4 cut(s) 665, 782, 922, 1169
Mox20I TGGCCA 1 cut(s) 404
MroXI GAANNNNTTC 2 cut(s) 69, 368
MscI TGGCCA 1 cut(s) 404
Msp20I TGGCCA 1 cut(s) 404
MspA1I CMGCKG 3 cut(s) 100, 1075, 1300
MspI CCGG 6 cut(s) 328, 973, 1106, 1141, 1189, 1216
MspR9I CCNGG 6 cut(s) 211, 310, 531, 895, 973, 1106
Mva1269I GAATGC 1 cut(s) 355
MvaI CCWGG 4 cut(s) 211, 310, 531, 895
MvnI CGCG 4 cut(s) 693, 1075, 1156, 1158
MwoI GCNNNNNNNGC 9 cut(s) 197, 266, 917, 1006, 1044, 1128, 1137, 1234, 1243
NciI CCSGG 2 cut(s) 973, 1106
NdeII GATC 6 cut(s) 13, 37, 232, 627, 732, 783
NlaIII CATG 5 cut(s) 46, 299, 634, 1024, 1294
NlaIV GGNNCC 8 cut(s) 739, 806, 844, 867, 868, 940, 1052, 1079
NmeAIII GCCGAG 1 cut(s) 1218
NspV TTCGAA 1 cut(s) 651
PagI TCATGA 2 cut(s) 42, 295
PceI AGGCCT 2 cut(s) 313, 548
PciSI GCTCTTC 2 cut(s) 545, 971
PcsI WCGNNNNNNNCGW 3 cut(s) 1160, 1169, 1245
PctI GAATGC 1 cut(s) 355
PdmI GAANNNNTTC 2 cut(s) 69, 368
PfeI GAWTC 2 cut(s) 305, 708
Pfl23II CGTACG 1 cut(s) 1197
PflFI GACNNNGTC 1 cut(s) 1027
PfoI TCCNGGA 1 cut(s) 209
PinAI ACCGGT 1 cut(s) 1215
PkrI GCNGC 9 cut(s) 922, 967, 1013, 1016, 1046, 1074, 1194, 1230, 1280
PleI GAGTC 4 cut(s) 664, 782, 922, 1168
PmaCI CACGTG 2 cut(s) 935, 1285
PmlI CACGTG 2 cut(s) 935, 1285
PpsI GAGTC 4 cut(s) 664, 782, 922, 1168
Ppu21I YACGTR 2 cut(s) 935, 1285
PpuMI RGGWCCY 2 cut(s) 866, 1050
Psp5II RGGWCCY 2 cut(s) 866, 1050
Psp6I CCWGG 4 cut(s) 209, 308, 529, 893
PspCI CACGTG 2 cut(s) 935, 1285
PspFI CCCAGC 2 cut(s) 1005, 1296
PspGI CCWGG 4 cut(s) 209, 308, 529, 893
PspLI CGTACG 1 cut(s) 1197
PspN4I GGNNCC 8 cut(s) 739, 806, 844, 867, 868, 940, 1052, 1079
PspOMI GGGCCC 1 cut(s) 842
PspPI GGNCC 5 cut(s) 842, 843, 866, 1050, 1077
PspPPI RGGWCCY 2 cut(s) 866, 1050
PstNI CAGNNNCTG 1 cut(s) 367
PsuI RGATCY 1 cut(s) 37
PsyI GACNNNGTC 1 cut(s) 1027
RsaI GTAC 4 cut(s) 524, 806, 1199, 1304
RsaNI GTAC 4 cut(s) 523, 805, 1198, 1303
SacII CCGCGG 1 cut(s) 1076
SalI GTCGAC 1 cut(s) 991
SapI GCTCTTC 2 cut(s) 545, 971
SatI GCNGC 9 cut(s) 921, 966, 1012, 1015, 1045, 1073, 1193, 1229, 1279
Sau3AI GATC 6 cut(s) 13, 37, 232, 627, 732, 783
Sau96I GGNCC 5 cut(s) 842, 843, 866, 1050, 1077
ScaI AGTACT 1 cut(s) 524
SchI GAGTC 4 cut(s) 665, 782, 922, 1169
ScrFI CCNGG 6 cut(s) 211, 310, 531, 895, 973, 1106
SduI GDGCHC 2 cut(s) 846, 1248
Sfr303I CCGCGG 1 cut(s) 1076
SfuI TTCGAA 1 cut(s) 651
SgrBI CCGCGG 1 cut(s) 1076
SinI GGWCC 3 cut(s) 866, 1050, 1077
SmlI CTYRAG 2 cut(s) 794, 1178
SmoI CTYRAG 2 cut(s) 794, 1178
Sse9I AATT 1 cut(s) 510
SseBI AGGCCT 2 cut(s) 313, 548
SspMI CTAG 2 cut(s) 375, 644
StuI AGGCCT 2 cut(s) 313, 548
StyD4I CCNGG 6 cut(s) 209, 308, 529, 893, 971, 1104
StyI CCWWGG 2 cut(s) 186, 834
TaaI ACNGT 6 cut(s) 106, 131, 223, 369, 603, 769
TaiI ACGT 8 cut(s) 288, 429, 827, 937, 1028, 1115, 1137, 1287
TasI AATT 1 cut(s) 510
TatI WGTACW 1 cut(s) 522
TauI GCSGC 7 cut(s) 923, 968, 1017, 1075, 1195, 1231, 1281
TfiI GAWTC 2 cut(s) 305, 708
TscAI CASTG 2 cut(s) 136, 226
TseI GCWGC 2 cut(s) 1011, 1044
TspDTI ATGAA 3 cut(s) 315, 500, 521
TspGWI ACGGA 2 cut(s) 555, 1160
TspRI CASTG 2 cut(s) 136, 226
Tth111I GACNNNGTC 1 cut(s) 1027
VpaK11BI GGWCC 3 cut(s) 866, 1050, 1077
XmiI GTMKAC 1 cut(s) 992
XmnI GAANNNNTTC 2 cut(s) 69, 368
XspI CTAG 2 cut(s) 375, 644
ZraI GACGTC 1 cut(s) 1026
ZrmI AGTACT 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.