Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0014951

Physical Location & Seq

Genomic Coordinates Forward (+)

18892211 .. 18893512

Gene Structure 1302 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1204900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1302 bp

ATGGGCCCCGGGGTCCCAGCAACCTCACCACCCACCTCCCGCGACGCCGCCACAACCACGCCCACCACCCTCCTCGGCAAATACCAACTGGGCCGGCTTTTGGGTCGAGGCAGCTTCGCTAAGGTCTACAAAGCCCAATCCCTCATCGACAACACCGCCGTCGCCATCAAAATCATCGACAAGTCCCAAGCCGACGCCGCAATGGAGCCCCGCATCCTTCGAGAAATCTCCGCCATGCGCCGCCTCCAGCAACACCCCAATGTCCTCAAAATCCACGAAGTCCTCGCCACCAAGTCCAAAATCTACCTCGTCGTGGAGCTCGCCACGGGCGGTGAGTTGTTCGCCAAAATCTCGCGTCATGGTAAATTTCCGGAGTCCCTTGCCCGCCGATACTTCCAGCAGCTCGTCTCTGCGTTGCACTTCTGCCACCAAAACGGCGTCGCCCACCGCGACGTGAAGCCCCAGAACCTTCTGCTGGACAAGAACGGCAACTTAAAAGTCTCGGATTTTGGACTCTCCGCTTTACCCGATCAGCTCAAAAACGGGCTGCTCCACACCGCCTGTGGGACCCCCGCGTACACGGCGCCCGAGGTGGTTTACCGGCGCGGCTACGACGGCTCCAAAGCCGACGCGTGGTCGTGCGGCGTCATCCTCTTCGTCCTGCTCGCCGGTCGCCTGCCTTTCGACGACAGCAATTTGATCGCTATGTATAAGAAAGTCCAGAGACGGGAGTACGAGATTCCGGCTTGGATTTCGAAACCGGCGGGTCGGATCATATACCAGCTGCTCGACCCGAACCCAAACACGAGGTTGGGGATCGAGGCGTTGATGGAGAAGCCGTGGTTTAAAAGATCTATTGATATAGGCCTTTTCGAGGAACCGGCAGCGGAGGTAAAGAAATACGACGTCGTTCCCTGCGGGATGAATGCGTTCGATATAATATCGATGTCGTCAGGGTTGGATCTGTCCGGGCTTTTCGAGGCGGGGACACGGAGGGAGAGGCGGTTCACGGCAGAATCGGCGGCGGAGACAGTGGTGGAGAAGGTGCGGGAGGTTGGGGATAAGCTGGGGTACAGGGTGGAGAGAGGGAAGAACGGTGTAAGTGTGGGAGTGGGGAGGGGAGCGATGATGAGGGTGGTAATGGCGGTTGAGGTAATGGAGATTGCACCGAGTTTGGTGGTTGGGGAAGTGAAGATGGTGGAGGGCGGAGGGGTTGAGTCTGAGCGGGAGGTTGGGCTTTGGGAAGAGTTGAGGGCTGGGCTGGAAAACCTTGTCGTTTCGTGGCATAGCGCTGCCGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009628 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko03060 ko03070 ko04113 ko04138 map03060 map03070 map04113 map04138

Pfam Domains

Protein Families

NAF Pkinase

Protein Analysis

434

Amino Acids

47.53

Weight (kDa)

9.05

Isoelectric Point (pI)

36.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	28 - 282	5.1e-72	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	29 - 278	7.7e-36	Protein tyrosine and serine/threonine kinase
NAF	PF03822	306 - 361	1.4e-19	NAF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014951)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23000 AT4G14580
fragaria_vesca	FvH4_4g02010
malus_domestica	MD13G1203400.v1.1 MD16G1204900.v1.1
prunus_persica	Prupe.1G026400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0388971
rosa_laevigata	RLG00000010002
rosa_multiflora	Rmu_sc0001395.1_g000009 Rmu_sc0012612.1_g000003
rosa_roxburghii	Rroxscaffold_5G00335300
rosa_rugosa	Rorug03G0321800.1
rosa_samantha	Rh4AG026300 Rh4BG019500 Rh4CG029300
rosa_wichuraiana	Rw4G001940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	909
AccB1I	GGYRCC	1 cut(s)	583
AccBSI	CCGCTC	1 cut(s)	1225
AccI	GTMKAC	1 cut(s)	126
AccII	CGCG	6 cut(s)	42, 355, 450, 575, 606, 632
AccIII	TCCGGA	1 cut(s)	370
AclWI	GGATC	3 cut(s)	779, 824, 969
AcsI	RAATTY	1 cut(s)	365
AcyI	GRCGYC	6 cut(s)	45, 195, 438, 584, 645, 906
AfaI	GTAC	3 cut(s)	578, 734, 1073
AfeI	AGCGCT	1 cut(s)	1291
AfiI	CCNNNNNNNGG	7 cut(s)	100, 313, 475, 564, 633, 727, 874
AflIII	ACRYGT	1 cut(s)	630
AhdI	GACNNNNNGTC	1 cut(s)	634
AjiI	CACGTC	1 cut(s)	454
AloI	GAACNNNNNNTCC	2 cut(s)	989, 1021
AluBI	AGCT	6 cut(s)	114, 319, 403, 535, 784, 1066
AluI	AGCT	6 cut(s)	114, 319, 403, 535, 784, 1066
Alw21I	GWGCWC	1 cut(s)	321
Alw26I	GTCTC	4 cut(s)	412, 505, 718, 1022
AlwI	GGATC	3 cut(s)	779, 824, 969
Ama87I	CYCGRG	2 cut(s)	8, 587
Aor13HI	TCCGGA	1 cut(s)	370
Aor51HI	AGCGCT	1 cut(s)	1291
AoxI	GGCC	3 cut(s)	4, 91, 865
ApaI	GGGCCC	1 cut(s)	8
ApeKI	GCWGC	6 cut(s)	111, 400, 547, 784, 884, 1292
ApoI	RAATTY	1 cut(s)	365
Asp700I	GAANNNNTTC	1 cut(s)	929
AspLEI	GCGC	4 cut(s)	240, 586, 606, 1292
AspS9I	GGNCC	5 cut(s)	4, 5, 13, 91, 567
AsuC2I	CCSGG	3 cut(s)	9, 10, 970
AsuHPI	GGTGA	2 cut(s)	18, 344
AsuII	TTCGAA	1 cut(s)	755
AvaI	CYCGRG	2 cut(s)	8, 587
AvaII	GGWCC	2 cut(s)	13, 567
BaeGI	GKGCMC	1 cut(s)	8
BanI	GGYRCC	1 cut(s)	583
BanII	GRGCYC	3 cut(s)	8, 210, 321
BauI	CACGAG	1 cut(s)	805
Bbv12I	GWGCWC	1 cut(s)	321
BbvI	GCAGC	6 cut(s)	123, 412, 534, 771, 896, 1279
BccI	CCATC	3 cut(s)	173, 823, 1189
BceAI	ACGGC	8 cut(s)	143, 451, 502, 597, 631, 823, 1026, 1280
BcgI	CGANNNNNNTGC	2 cut(s)	682, 716
BcnI	CCSGG	3 cut(s)	9, 10, 970
BcoDI	GTCTC	4 cut(s)	412, 505, 718, 1022
BfoI	RGCGCY	2 cut(s)	587, 1293
BglII	AGATCT	1 cut(s)	851
Bme1390I	CCNGG	3 cut(s)	9, 10, 970
Bme18I	GGWCC	2 cut(s)	13, 567
BmeRI	GACNNNNNGTC	1 cut(s)	634
BmeT110I	CYCGRG	2 cut(s)	8, 587
BmgBI	CACGTC	1 cut(s)	454
BmgT120I	GGNCC	5 cut(s)	4, 5, 13, 91, 567
BmrFI	CCNGG	3 cut(s)	9, 10, 970
BmrI	ACTGGG	1 cut(s)	98
BmsI	GCATC	1 cut(s)	222
BmuI	ACTGGG	1 cut(s)	98
BpmI	CTGGAG	1 cut(s)	230
Bpu10I	CCTNAGC	1 cut(s)	120
Bpu14I	TTCGAA	1 cut(s)	755
BpuMI	CCSGG	3 cut(s)	9, 10, 970
Bsa29I	ATCGAT	1 cut(s)	944
BsaHI	GRCGYC	6 cut(s)	45, 195, 438, 584, 645, 906
BsaJI	CCNNGG	7 cut(s)	7, 8, 9, 73, 324, 588, 839
BsaWI	WCCGGW	1 cut(s)	370
BsaXI	ACNNNNNCTCC	6 cut(s)	602, 632, 824, 854, 989, 1019
Bsc4I	CCNNNNNNNGG	7 cut(s)	100, 313, 475, 564, 633, 727, 874
Bse118I	RCCGGY	5 cut(s)	93, 600, 668, 760, 880
Bse1I	ACTGG	1 cut(s)	93
Bse3DI	GCAATG	1 cut(s)	207
BseAI	TCCGGA	1 cut(s)	370
BseCI	ATCGAT	1 cut(s)	944
BseDI	CCNNGG	7 cut(s)	7, 8, 9, 73, 324, 588, 839
BseGI	GGATG	3 cut(s)	213, 648, 927
BseLI	CCNNNNNNNGG	7 cut(s)	100, 313, 475, 564, 633, 727, 874
BseMI	GCAATG	1 cut(s)	207
BseMII	CTCAG	1 cut(s)	1212
BseNI	ACTGG	1 cut(s)	93
BseRI	GAGGAG	1 cut(s)	62
BseSI	GKGCMC	1 cut(s)	8
BseXI	GCAGC	6 cut(s)	123, 412, 534, 771, 896, 1279
BseYI	CCCAGC	3 cut(s)	16, 1066, 1256
Bsh1236I	CGCG	6 cut(s)	42, 355, 450, 575, 606, 632
Bsh1285I	CGRYCG	1 cut(s)	673
BshFI	GGCC	3 cut(s)	6, 93, 867
BshNI	GGYRCC	1 cut(s)	583
BshVI	ATCGAT	1 cut(s)	944
BsiEI	CGRYCG	1 cut(s)	673
BsiHKAI	GWGCWC	1 cut(s)	321
BsiHKCI	CYCGRG	2 cut(s)	8, 587
BsiSI	CCGG	9 cut(s)	9, 94, 371, 601, 669, 743, 761, 881, 969
BslFI	GGGAC	4 cut(s)	169, 361, 580, 1000
BslI	CCNNNNNNNGG	7 cut(s)	100, 313, 475, 564, 633, 727, 874
BsmAI	GTCTC	4 cut(s)	412, 505, 718, 1022
BsmBI	CGTCTC	2 cut(s)	412, 718
BsmFI	GGGAC	4 cut(s)	169, 361, 580, 1000
BsmI	GAATGC	1 cut(s)	931
BsnI	GGCC	3 cut(s)	6, 93, 867
BsoBI	CYCGRG	2 cut(s)	8, 587
Bsp119I	TTCGAA	1 cut(s)	755
Bsp120I	GGGCCC	1 cut(s)	4
Bsp1286I	GDGCHC	3 cut(s)	8, 210, 321
Bsp13I	TCCGGA	1 cut(s)	370
Bsp143I	GATC	6 cut(s)	529, 699, 771, 816, 851, 961
BspANI	GGCC	3 cut(s)	6, 93, 867
BspCNI	CTCAG	1 cut(s)	1213
BspDI	ATCGAT	1 cut(s)	944
BspEI	TCCGGA	1 cut(s)	370
BspFNI	CGCG	6 cut(s)	42, 355, 450, 575, 606, 632
BspPI	GGATC	3 cut(s)	779, 824, 969
BspT104I	TTCGAA	1 cut(s)	755
BspT107I	GGYRCC	1 cut(s)	583
BsrBI	CCGCTC	1 cut(s)	1225
BsrDI	GCAATG	1 cut(s)	207
BsrFI	RCCGGY	5 cut(s)	93, 600, 668, 760, 880
BsrI	ACTGG	1 cut(s)	93
BssAI	RCCGGY	5 cut(s)	93, 600, 668, 760, 880
BssECI	CCNNGG	7 cut(s)	7, 8, 9, 73, 324, 588, 839
BssMI	GATC	6 cut(s)	529, 699, 771, 816, 851, 961
BssNI	GRCGYC	6 cut(s)	45, 195, 438, 584, 645, 906
BssSI	CACGAG	1 cut(s)	805
Bst2BI	CACGAG	1 cut(s)	805
Bst4CI	ACNGT	2 cut(s)	1033, 1097
Bst6I	CTCTTC	2 cut(s)	659, 1239
BstACI	GRCGYC	6 cut(s)	45, 195, 438, 584, 645, 906
BstBI	TTCGAA	1 cut(s)	755
BstC8I	GCNNGC	5 cut(s)	95, 321, 385, 666, 677
BstDEI	CTNAG	2 cut(s)	120, 1221
BstDSI	CCRYGG	2 cut(s)	324, 839
BstENI	CCTNNNNNAGG	1 cut(s)	872
BstF5I	GGATG	3 cut(s)	213, 648, 927
BstFNI	CGCG	6 cut(s)	42, 355, 450, 575, 606, 632
BstH2I	RGCGCY	2 cut(s)	587, 1293
BstHHI	GCGC	4 cut(s)	240, 586, 606, 1292
BstKTI	GATC	6 cut(s)	532, 702, 774, 819, 854, 964
BstMAI	GTCTC	4 cut(s)	412, 505, 718, 1022
BstMBI	GATC	6 cut(s)	529, 699, 771, 816, 851, 961
BstMCI	CGRYCG	1 cut(s)	673
BstMWI	GCNNNNNNNGC	4 cut(s)	197, 581, 615, 1019
BstSCI	CCNGG	3 cut(s)	7, 8, 968
BstSLI	GKGCMC	1 cut(s)	8
BstUI	CGCG	6 cut(s)	42, 355, 450, 575, 606, 632
BstV1I	GCAGC	6 cut(s)	123, 412, 534, 771, 896, 1279
BstX2I	RGATCY	2 cut(s)	851, 961
BstYI	RGATCY	2 cut(s)	851, 961
Bsu15I	ATCGAT	1 cut(s)	944
BsuRI	GGCC	3 cut(s)	6, 93, 867
BsuTUI	ATCGAT	1 cut(s)	944
BtgI	CCRYGG	2 cut(s)	324, 839
BtgZI	GCGATG	1 cut(s)	1139
BtrI	CACGTC	1 cut(s)	454
BtsCI	GGATG	3 cut(s)	213, 648, 927
BtsIMutI	CAGTG	1 cut(s)	1038
Cac8I	GCNNGC	5 cut(s)	95, 321, 385, 666, 677
CfoI	GCGC	4 cut(s)	240, 586, 606, 1292
Cfr10I	RCCGGY	5 cut(s)	93, 600, 668, 760, 880
Cfr13I	GGNCC	5 cut(s)	4, 5, 13, 91, 567
Cfr9I	CCCGGG	1 cut(s)	8
ClaI	ATCGAT	1 cut(s)	944
CseI	GACGC	6 cut(s)	53, 203, 344, 427, 634, 638
Csp6I	GTAC	3 cut(s)	577, 733, 1072
CviAII	CATG	2 cut(s)	235, 359
CviQI	GTAC	3 cut(s)	577, 733, 1072
DdeI	CTNAG	2 cut(s)	120, 1221
DinI	GGCGCC	1 cut(s)	585
DpnI	GATC	6 cut(s)	531, 701, 773, 818, 853, 963
DpnII	GATC	6 cut(s)	529, 699, 771, 816, 851, 961
DraI	TTTAAA	1 cut(s)	847
DriI	GACNNNNNGTC	1 cut(s)	634
Eam1104I	CTCTTC	2 cut(s)	659, 1239
Eam1105I	GACNNNNNGTC	1 cut(s)	634
EarI	CTCTTC	2 cut(s)	659, 1239
EciI	GGCGGA	3 cut(s)	220, 1040, 1221
Ecl136II	GAGCTC	1 cut(s)	319
Eco147I	AGGCCT	1 cut(s)	867
Eco24I	GRGCYC	3 cut(s)	8, 210, 321
Eco47I	GGWCC	2 cut(s)	13, 567
Eco47III	AGCGCT	1 cut(s)	1291
Eco53kI	GAGCTC	1 cut(s)	319
Eco88I	CYCGRG	2 cut(s)	8, 587
EcoICRI	GAGCTC	1 cut(s)	319
EcoNI	CCTNNNNNAGG	1 cut(s)	872
EcoO109I	RGGNCCY	3 cut(s)	5, 13, 567
EcoT38I	GRGCYC	3 cut(s)	8, 210, 321
EgeI	GGCGCC	1 cut(s)	585
EheI	GGCGCC	1 cut(s)	585
Esp3I	CGTCTC	2 cut(s)	412, 718
FaeI	CATG	2 cut(s)	238, 362
FaiI	YATR	9 cut(s)	236, 360, 707, 711, 776, 778, 863, 938, 1287
FaqI	GGGAC	4 cut(s)	169, 361, 580, 1000
FatI	CATG	2 cut(s)	234, 358
FauI	CCCGC	9 cut(s)	47, 218, 392, 580, 757, 911, 976, 1041, 1218
FblI	GTMKAC	1 cut(s)	126
FokI	GGATG	3 cut(s)	200, 635, 934
FriOI	GRGCYC	3 cut(s)	8, 210, 321
GlaI	GCGC	4 cut(s)	239, 585, 605, 1291
GsaI	CCCAGC	3 cut(s)	20, 1070, 1260
GsuI	CTGGAG	1 cut(s)	230
HaeII	RGCGCY	2 cut(s)	587, 1293
HaeIII	GGCC	3 cut(s)	6, 93, 867
HapII	CCGG	9 cut(s)	9, 94, 371, 601, 669, 743, 761, 881, 969
HgaI	GACGC	6 cut(s)	53, 203, 344, 427, 634, 638
HhaI	GCGC	4 cut(s)	240, 586, 606, 1292
Hin1I	GRCGYC	6 cut(s)	45, 195, 438, 584, 645, 906
Hin1II	CATG	2 cut(s)	238, 362
Hin6I	GCGC	4 cut(s)	238, 584, 604, 1290
HinP1I	GCGC	4 cut(s)	238, 584, 604, 1290
HinfI	GANTC	5 cut(s)	374, 513, 739, 1016, 1217
HpaII	CCGG	9 cut(s)	9, 94, 371, 601, 669, 743, 761, 881, 969
HphI	GGTGA	2 cut(s)	18, 344
Hpy166II	GTNNAC	4 cut(s)	127, 579, 598, 1008
Hpy188I	TCNGA	3 cut(s)	505, 771, 1222
Hpy188III	TCNNGA	3 cut(s)	221, 371, 721
Hpy8I	GTNNAC	4 cut(s)	127, 579, 598, 1008
HpyAV	CCTTC	3 cut(s)	227, 479, 1036
HpyCH4III	ACNGT	2 cut(s)	1033, 1097
HpyCH4IV	ACGT	2 cut(s)	453, 906
HpyCH4V	TGCA	2 cut(s)	418, 1166
HpyF10VI	GCNNNNNNNGC	4 cut(s)	197, 581, 615, 1019
HpyF3I	CTNAG	2 cut(s)	120, 1221
HpySE526I	ACGT	2 cut(s)	453, 906
Hsp92I	GRCGYC	6 cut(s)	45, 195, 438, 584, 645, 906
Hsp92II	CATG	2 cut(s)	238, 362
HspAI	GCGC	4 cut(s)	238, 584, 604, 1290
KasI	GGCGCC	1 cut(s)	583
KflI	GGGWCCC	2 cut(s)	13, 567
Kpn2I	TCCGGA	1 cut(s)	370
KroI	GCCGGC	1 cut(s)	93
KroNI	GCCGGC	1 cut(s)	95
Kzo9I	GATC	6 cut(s)	529, 699, 771, 816, 851, 961
LmnI	GCTCC	5 cut(s)	205, 316, 555, 623, 1121
Lsp1109I	GCAGC	6 cut(s)	123, 412, 534, 771, 896, 1279
LweI	GCATC	1 cut(s)	222
MaeII	ACGT	2 cut(s)	453, 906
MalI	GATC	6 cut(s)	531, 701, 773, 818, 853, 963
MbiI	CCGCTC	1 cut(s)	1225
MboI	GATC	6 cut(s)	529, 699, 771, 816, 851, 961
MboII	GAAGA	4 cut(s)	646, 1102, 1204, 1256
MflI	RGATCY	2 cut(s)	851, 961
MhlI	GDGCHC	3 cut(s)	8, 210, 321
MluCI	AATT	2 cut(s)	365, 694
MluI	ACGCGT	1 cut(s)	630
Mly113I	GGCGCC	1 cut(s)	584
MlyI	GAGTC	3 cut(s)	383, 507, 1226
MmeI	TCCRAC	2 cut(s)	749, 939
MroI	TCCGGA	1 cut(s)	370
MroNI	GCCGGC	1 cut(s)	93
MroXI	GAANNNNTTC	1 cut(s)	929
MseI	TTAA	2 cut(s)	494, 846
MslI	CAYNNNNRTG	1 cut(s)	258
MspA1I	CMGCKG	2 cut(s)	784, 887
MspI	CCGG	9 cut(s)	9, 94, 371, 601, 669, 743, 761, 881, 969
MspR9I	CCNGG	3 cut(s)	9, 10, 970
Mva1269I	GAATGC	1 cut(s)	931
MvnI	CGCG	6 cut(s)	42, 355, 450, 575, 606, 632
MwoI	GCNNNNNNNGC	4 cut(s)	197, 581, 615, 1019
NaeI	GCCGGC	1 cut(s)	95
NarI	GGCGCC	1 cut(s)	584
NciI	CCSGG	3 cut(s)	9, 10, 970
NdeII	GATC	6 cut(s)	529, 699, 771, 816, 851, 961
NgoMIV	GCCGGC	1 cut(s)	93
NlaIII	CATG	2 cut(s)	238, 362
NmeAIII	GCCGAG	1 cut(s)	54
NspV	TTCGAA	1 cut(s)	755
PceI	AGGCCT	1 cut(s)	867
PcsI	WCGNNNNNNNCGW	1 cut(s)	447
PctI	GAATGC	1 cut(s)	931
PdiI	GCCGGC	1 cut(s)	95
PdmI	GAANNNNTTC	1 cut(s)	929
PfeI	GAWTC	2 cut(s)	739, 1016
PleI	GAGTC	3 cut(s)	382, 507, 1225
PluTI	GGCGCC	1 cut(s)	587
PpsI	GAGTC	3 cut(s)	382, 507, 1225
PpuMI	RGGWCCY	2 cut(s)	13, 567
Psp124BI	GAGCTC	1 cut(s)	321
Psp5II	RGGWCCY	2 cut(s)	13, 567
PspFI	CCCAGC	3 cut(s)	16, 1066, 1256
PspOMI	GGGCCC	1 cut(s)	4
PspPI	GGNCC	5 cut(s)	4, 5, 13, 91, 567
PspPPI	RGGWCCY	2 cut(s)	13, 567
PsuI	RGATCY	2 cut(s)	851, 961
PvuII	CAGCTG	1 cut(s)	784
RsaI	GTAC	3 cut(s)	578, 734, 1073
RsaNI	GTAC	3 cut(s)	577, 733, 1072
RseI	CAYNNNNRTG	1 cut(s)	258
SacI	GAGCTC	1 cut(s)	321
SaqAI	TTAA	2 cut(s)	494, 846
Sau3AI	GATC	6 cut(s)	529, 699, 771, 816, 851, 961
Sau96I	GGNCC	5 cut(s)	4, 5, 13, 91, 567
SchI	GAGTC	3 cut(s)	383, 507, 1226
ScrFI	CCNGG	3 cut(s)	9, 10, 970
SduI	GDGCHC	3 cut(s)	8, 210, 321
SfaNI	GCATC	1 cut(s)	222
SfoI	GGCGCC	1 cut(s)	585
SfuI	TTCGAA	1 cut(s)	755
SinI	GGWCC	2 cut(s)	13, 567
SmaI	CCCGGG	1 cut(s)	10
SmiMI	CAYNNNNRTG	1 cut(s)	258
Sse9I	AATT	2 cut(s)	365, 694
SseBI	AGGCCT	1 cut(s)	867
SspDI	GGCGCC	1 cut(s)	583
SstI	GAGCTC	1 cut(s)	321
StuI	AGGCCT	1 cut(s)	867
StyD4I	CCNGG	3 cut(s)	7, 8, 968
TaaI	ACNGT	2 cut(s)	1033, 1097
TaiI	ACGT	2 cut(s)	456, 909
TasI	AATT	2 cut(s)	365, 694
TauI	GCSGC	6 cut(s)	50, 200, 243, 609, 645, 1025
TfiI	GAWTC	2 cut(s)	739, 1016
Tru1I	TTAA	2 cut(s)	494, 846
Tru9I	TTAA	2 cut(s)	494, 846
TscAI	CASTG	1 cut(s)	1038
TseI	GCWGC	6 cut(s)	111, 400, 547, 784, 884, 1292
TspDTI	ATGAA	1 cut(s)	938
TspGWI	ACGGA	1 cut(s)	1006
TspMI	CCCGGG	1 cut(s)	8
TspRI	CASTG	1 cut(s)	1038
VpaK11BI	GGWCC	2 cut(s)	13, 567
XagI	CCTNNNNNAGG	1 cut(s)	872
XapI	RAATTY	1 cut(s)	365
XcmI	CCANNNNNNNNNTGG	1 cut(s)	560
XmaI	CCCGGG	1 cut(s)	8
XmiI	GTMKAC	1 cut(s)	126
XmnI	GAANNNNTTC	1 cut(s)	929
ZraI	GACGTC	1 cut(s)	907

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.