Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0014951

Physical Location & Seq

Genomic Coordinates Forward (+)

2729910 .. 2731390

Gene Structure 1481 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00335300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1344 bp

ATGAGCCATGGGCCGTGGGCCCCCACCACCCCCTCCGCCGCCTCCACCACCACCACCACCACCACCAACACCACCCTTCTCGGCAAGTACCAACTGGGCCGGCTACTGGGCCGGGGCAGCTTCGCCAAGGTCTACAAGGCCCAGTCCTTAGCCGACGAAACCCCCGTCGCCATTAAAATCATCGACAAGCTCAAAACCGACGCAAATATGGAGCCTCTGATACTCCGGGAGATCTCCGCCATGCGCCGCCTCCAGGACCACCCTAACATCCTCAAAATCCACGAGGTCATGGCCACAAAGTCCAAGATCTACATCGTCGTCGAGCTAGCCACCGGCGGCGAGCTCTTCGCCAAGATCTCCCGCTACGGAAAGTTGCCGGAATCCCTCGCCCGCCGCTACTTCCAGCAGCTCGTCTCCGCCCTCCGCTTCTGCCACGAAAACGGCGTCGCTCACCGCGACGTGAAGCCGCAGAACCTCCTCCTCGACGGCAACGGAAACCTCAAGGTCTCCGATTTCGGACTCTCCGCCCTGCCGGAGCAGCTCAAAAACGGCCTCCTCCACACGGCTTGTGGAACTCCGGCGTACACGGCGCCGGAGGTTCTGTACCGGGTCGGGTACGAAGGATCCAAGGCCGACGCGTGGTCCTGCGGAGTCATCCTCTTCGTCCTCCTCGCCGGACACTTGCCGTTCGACGACAGCAATCTGGTGGCGATGCATAAGAAGATTCAGCGGCGGGATTACGTCATCCCGGCGACGATTTCGAAGCCGGCGCGGCGGATCATATACCAGCTGCTCGACCCGAACCCGAACACGAGGCTGAGCATAGAGGCCGTCACGGAAAAGGCGTGGTTCCAGAAGGCGATAGATTTGAAGATGTTAGTTTCCGATAGCTGCGGTATAATCGAGGCGGAGAAATCTCTACCACCGAGCAAATGCGACGTCGTTTCGGGGATGAACGCGTTCGATATAATCTCCATGTCGTCGGGGCTGGATTTGTCGGGGCTTTTCGAGGCGGAGAACAGGAGCGAGAGGCGGTTCACGGCGAATGTGGCGGCGGAGAAGGTGGTGGAGAAGGTGGGGGAGGTTGGGGAGAGGCTCGGGTACAAAGCGGAGAGAGGGAAAGGTGGGATGAGCGTTGGGTTGGGGAAAGGAGGGAAAGGGAAGGGACGACGTGCCGTTTTGACGGTGGAAATGATGGAGGTGGCGGCGGGTTTGGTTTTAGGGGAAGTGAAGATGGTGGAGGGTGGGGTGGAGTTTCCCGAGCTTCTTTGGGAGGACTTGAAAACTGGGTTAGGGGACGTCGTGGTGACGTGGTGTCATGGCAAAACGGTGGCGTTTAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009628 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko03060 ko03070 ko04113 ko04138 map03060 map03070 map04113 map04138

Pfam Domains

Protein Families

NAF Pkinase

Protein Analysis

447

Amino Acids

48.71

Weight (kDa)

8.99

Isoelectric Point (pI)

36.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	30 - 284	3.3e-70	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	31 - 280	8.1e-36	Protein tyrosine and serine/threonine kinase
NAF	PF03822	318 - 371	1.2e-18	NAF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014951)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23000 AT4G14580
fragaria_vesca	FvH4_4g02010
malus_domestica	MD13G1203400.v1.1 MD16G1204900.v1.1
prunus_persica	Prupe.1G026400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0388971
rosa_laevigata	RLG00000010002
rosa_multiflora	Rmu_sc0001395.1_g000009 Rmu_sc0012612.1_g000003
rosa_roxburghii	Rroxscaffold_5G00335300
rosa_rugosa	Rorug03G0321800.1
rosa_samantha	Rh4AG026300 Rh4BG019500 Rh4CG029300
rosa_wichuraiana	Rw4G001940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	942, 1302
AccB1I	GGYRCC	1 cut(s)	589
AccI	GTMKAC	1 cut(s)	132
AccII	CGCG	4 cut(s)	456, 638, 772, 959
AclWI	GGATC	3 cut(s)	618, 631, 785
AcoI	YGGCCR	1 cut(s)	291
AcyI	GRCGYC	4 cut(s)	444, 590, 939, 1299
AfaI	GTAC	5 cut(s)	89, 584, 605, 617, 1103
AfiI	CCNNNNNNNGG	4 cut(s)	106, 253, 562, 639
AflIII	ACRYGT	2 cut(s)	636, 957
AgsI	TTSAA	2 cut(s)	871, 1282
AhdI	GACNNNNNGTC	2 cut(s)	640, 1314
AjiI	CACGTC	3 cut(s)	460, 1172, 1311
AjnI	CCWGG	1 cut(s)	252
AluBI	AGCT	9 cut(s)	120, 190, 325, 343, 409, 541, 790, 891, 1264
AluI	AGCT	9 cut(s)	120, 190, 325, 343, 409, 541, 790, 891, 1264
Alw21I	GWGCWC	1 cut(s)	345
Alw26I	GTCTC	2 cut(s)	418, 511
AlwI	GGATC	3 cut(s)	618, 631, 785
Ama87I	CYCGRG	2 cut(s)	1097, 1259
AoxI	GGCC	9 cut(s)	11, 18, 97, 109, 138, 291, 550, 630, 828
ApaI	GGGCCC	1 cut(s)	22
ApeKI	GCWGC	5 cut(s)	117, 406, 538, 790, 891
ArsI	GACNNNNNNTTYG	6 cut(s)	150, 182, 429, 461, 924, 956
Asp700I	GAANNNNTTC	1 cut(s)	959
AspLEI	GCGC	3 cut(s)	246, 592, 772
AspS9I	GGNCC	8 cut(s)	11, 18, 19, 97, 109, 139, 256, 642
AsuC2I	CCSGG	4 cut(s)	113, 227, 608, 749
AsuHPI	GGTGA	2 cut(s)	443, 1318
AsuII	TTCGAA	1 cut(s)	761
AsuNHI	GCTAGC	1 cut(s)	325
AvaI	CYCGRG	2 cut(s)	1097, 1259
AvaII	GGWCC	2 cut(s)	256, 642
BaeGI	GKGCMC	1 cut(s)	22
BalI	TGGCCA	1 cut(s)	293
BamHI	GGATCC	1 cut(s)	623
BanI	GGYRCC	1 cut(s)	589
BanII	GRGCYC	2 cut(s)	22, 345
BauI	CACGAG	2 cut(s)	281, 811
Bbv12I	GWGCWC	1 cut(s)	345
BbvI	GCAGC	5 cut(s)	129, 418, 550, 777, 878
BccI	CCATC	2 cut(s)	1189, 1228
BceAI	ACGGC	9 cut(s)	457, 502, 565, 579, 603, 670, 815, 1056, 1160
BciT130I	CCWGG	1 cut(s)	254
BcnI	CCSGG	4 cut(s)	113, 227, 608, 749
BcoDI	GTCTC	2 cut(s)	418, 511
BfaI	CTAG	1 cut(s)	326
BfoI	RGCGCY	1 cut(s)	593
BglI	GCCNNNNNGGC	1 cut(s)	772
BglII	AGATCT	3 cut(s)	231, 306, 354
BlpI	GCTNAGC	1 cut(s)	818
Bme1390I	CCNGG	5 cut(s)	113, 227, 254, 608, 749
Bme18I	GGWCC	2 cut(s)	256, 642
BmeRI	GACNNNNNGTC	2 cut(s)	640, 1314
BmeT110I	CYCGRG	2 cut(s)	1097, 1259
BmgBI	CACGTC	3 cut(s)	460, 1172, 1311
BmgT120I	GGNCC	8 cut(s)	11, 18, 19, 97, 109, 139, 256, 642
BmiI	GGNNCC	6 cut(s)	20, 21, 213, 591, 625, 851
BmrFI	CCNGG	5 cut(s)	113, 227, 254, 608, 749
BmrI	ACTGGG	4 cut(s)	104, 116, 136, 1296
BmsI	GCATC	1 cut(s)	702
BmtI	GCTAGC	1 cut(s)	329
BmuI	ACTGGG	4 cut(s)	104, 116, 136, 1296
BplI	GAGNNNNNCTC	2 cut(s)	642, 674
BpmI	CTGGAG	1 cut(s)	236
Bpu10I	CCTNAGC	1 cut(s)	148
Bpu1102I	GCTNAGC	1 cut(s)	818
Bpu14I	TTCGAA	1 cut(s)	761
BpuEI	CTTGAG	1 cut(s)	485
BpuMI	CCSGG	4 cut(s)	113, 227, 608, 749
BsaBI	GATNNNNATC	1 cut(s)	311
BsaHI	GRCGYC	4 cut(s)	444, 590, 939, 1299
BsaI	GGTCTC	1 cut(s)	511
BsaJI	CCNNGG	5 cut(s)	7, 14, 112, 126, 627
BsaXI	ACNNNNNCTCC	2 cut(s)	587, 617
Bsc4I	CCNNNNNNNGG	4 cut(s)	106, 253, 562, 639
Bse118I	RCCGGY	3 cut(s)	99, 332, 766
Bse1I	ACTGG	4 cut(s)	99, 111, 142, 1291
Bse8I	GATNNNNATC	1 cut(s)	311
BseBI	CCWGG	1 cut(s)	254
BseDI	CCNNGG	5 cut(s)	7, 14, 112, 126, 627
BseGI	GGATG	5 cut(s)	267, 654, 744, 957, 1134
BseJI	GATNNNNATC	1 cut(s)	311
BseLI	CCNNNNNNNGG	4 cut(s)	106, 253, 562, 639
BseMII	CTCAG	1 cut(s)	809
BseNI	ACTGG	4 cut(s)	99, 111, 142, 1291
BseRI	GAGGAG	4 cut(s)	467, 470, 545, 659
BseSI	GKGCMC	1 cut(s)	22
BseXI	GCAGC	5 cut(s)	129, 418, 550, 777, 878
Bsh1236I	CGCG	4 cut(s)	456, 638, 772, 959
BshFI	GGCC	9 cut(s)	13, 20, 99, 111, 140, 293, 552, 632, 830
BshNI	GGYRCC	1 cut(s)	589
BsiHKAI	GWGCWC	1 cut(s)	345
BsiHKCI	CYCGRG	2 cut(s)	1097, 1259
BslFI	GGGAC	2 cut(s)	1179, 1310
BslI	CCNNNNNNNGG	4 cut(s)	106, 253, 562, 639
BsmAI	GTCTC	2 cut(s)	418, 511
BsmBI	CGTCTC	1 cut(s)	418
BsmFI	GGGAC	2 cut(s)	1179, 1310
BsnI	GGCC	9 cut(s)	13, 20, 99, 111, 140, 293, 552, 632, 830
Bso31I	GGTCTC	1 cut(s)	511
BsoBI	CYCGRG	2 cut(s)	1097, 1259
Bsp119I	TTCGAA	1 cut(s)	761
Bsp120I	GGGCCC	1 cut(s)	18
Bsp1286I	GDGCHC	2 cut(s)	22, 345
Bsp143I	GATC	5 cut(s)	231, 306, 354, 623, 777
Bsp1720I	GCTNAGC	1 cut(s)	818
Bsp19I	CCATGG	1 cut(s)	7
BspANI	GGCC	9 cut(s)	13, 20, 99, 111, 140, 293, 552, 632, 830
BspCNI	CTCAG	1 cut(s)	810
BspFNI	CGCG	4 cut(s)	456, 638, 772, 959
BspLI	GGNNCC	6 cut(s)	20, 21, 213, 591, 625, 851
BspOI	GCTAGC	1 cut(s)	329
BspPI	GGATC	3 cut(s)	618, 631, 785
BspQI	GCTCTTC	1 cut(s)	350
BspT104I	TTCGAA	1 cut(s)	761
BspT107I	GGYRCC	1 cut(s)	589
BspTNI	GGTCTC	1 cut(s)	511
BsrFI	RCCGGY	3 cut(s)	99, 332, 766
BsrI	ACTGG	4 cut(s)	99, 111, 142, 1291
BssAI	RCCGGY	3 cut(s)	99, 332, 766
BssECI	CCNNGG	5 cut(s)	7, 14, 112, 126, 627
BssMI	GATC	5 cut(s)	231, 306, 354, 623, 777
BssNI	GRCGYC	4 cut(s)	444, 590, 939, 1299
BssSI	CACGAG	2 cut(s)	281, 811
BssT1I	CCWWGG	3 cut(s)	7, 126, 627
Bst2BI	CACGAG	2 cut(s)	281, 811
Bst2UI	CCWGG	1 cut(s)	254
Bst4CI	ACNGT	2 cut(s)	1186, 1330
Bst6I	CTCTTC	2 cut(s)	350, 665
BstACI	GRCGYC	4 cut(s)	444, 590, 939, 1299
BstBI	TTCGAA	1 cut(s)	761
BstC8I	GCNNGC	5 cut(s)	101, 327, 341, 391, 768
BstDEI	CTNAG	2 cut(s)	148, 818
BstDSI	CCRYGG	2 cut(s)	7, 14
BstF5I	GGATG	5 cut(s)	267, 654, 744, 957, 1134
BstFNI	CGCG	4 cut(s)	456, 638, 772, 959
BstH2I	RGCGCY	1 cut(s)	593
BstHHI	GCGC	3 cut(s)	246, 592, 772
BstKTI	GATC	5 cut(s)	234, 309, 357, 626, 780
BstMAI	GTCTC	2 cut(s)	418, 511
BstMBI	GATC	5 cut(s)	231, 306, 354, 623, 777
BstMWI	GCNNNNNNNGC	5 cut(s)	117, 538, 587, 772, 1049
BstNI	CCWGG	1 cut(s)	254
BstSCI	CCNGG	5 cut(s)	111, 225, 252, 606, 747
BstSLI	GKGCMC	1 cut(s)	22
BstUI	CGCG	4 cut(s)	456, 638, 772, 959
BstV1I	GCAGC	5 cut(s)	129, 418, 550, 777, 878
BstX2I	RGATCY	4 cut(s)	231, 306, 354, 623
BstYI	RGATCY	4 cut(s)	231, 306, 354, 623
BsuRI	GGCC	9 cut(s)	13, 20, 99, 111, 140, 293, 552, 632, 830
BtgI	CCRYGG	2 cut(s)	7, 14
BtgZI	GCGATG	1 cut(s)	725
BtrI	CACGTC	3 cut(s)	460, 1172, 1311
BtsCI	GGATG	5 cut(s)	267, 654, 744, 957, 1134
Cac8I	GCNNGC	5 cut(s)	101, 327, 341, 391, 768
CfoI	GCGC	3 cut(s)	246, 592, 772
Cfr10I	RCCGGY	3 cut(s)	99, 332, 766
Cfr13I	GGNCC	8 cut(s)	11, 18, 19, 97, 109, 139, 256, 642
CseI	GACGC	3 cut(s)	209, 433, 644
Csp6I	GTAC	5 cut(s)	88, 583, 604, 616, 1102
CspCI	CAANNNNNGTGG	2 cut(s)	548, 583
CviAII	CATG	5 cut(s)	8, 241, 289, 976, 1319
CviQI	GTAC	5 cut(s)	88, 583, 604, 616, 1102
DdeI	CTNAG	2 cut(s)	148, 818
DinI	GGCGCC	1 cut(s)	591
DpnI	GATC	5 cut(s)	233, 308, 356, 625, 779
DpnII	GATC	5 cut(s)	231, 306, 354, 623, 777
DriI	GACNNNNNGTC	2 cut(s)	640, 1314
EaeI	YGGCCR	1 cut(s)	291
Eam1104I	CTCTTC	2 cut(s)	350, 665
Eam1105I	GACNNNNNGTC	2 cut(s)	640, 1314
EarI	CTCTTC	2 cut(s)	350, 665
EciI	GGCGGA	8 cut(s)	25, 226, 406, 514, 790, 923, 1028, 1070
Ecl136II	GAGCTC	1 cut(s)	343
Eco130I	CCWWGG	3 cut(s)	7, 126, 627
Eco24I	GRGCYC	2 cut(s)	22, 345
Eco31I	GGTCTC	1 cut(s)	511
Eco47I	GGWCC	2 cut(s)	256, 642
Eco53kI	GAGCTC	1 cut(s)	343
Eco88I	CYCGRG	2 cut(s)	1097, 1259
EcoICRI	GAGCTC	1 cut(s)	343
EcoO109I	RGGNCCY	1 cut(s)	19
EcoRII	CCWGG	1 cut(s)	252
EcoT14I	CCWWGG	3 cut(s)	7, 126, 627
EcoT22I	ATGCAT	1 cut(s)	717
EcoT38I	GRGCYC	2 cut(s)	22, 345
EgeI	GGCGCC	1 cut(s)	591
EheI	GGCGCC	1 cut(s)	591
ErhI	CCWWGG	3 cut(s)	7, 126, 627
Esp3I	CGTCTC	1 cut(s)	418
FaeI	CATG	5 cut(s)	11, 244, 292, 979, 1322
FaqI	GGGAC	2 cut(s)	1179, 1310
FatI	CATG	5 cut(s)	7, 240, 288, 975, 1318
FauI	CCCGC	4 cut(s)	368, 398, 726, 1201
FblI	GTMKAC	1 cut(s)	132
FokI	GGATG	5 cut(s)	254, 641, 731, 964, 1141
FriOI	GRGCYC	2 cut(s)	22, 345
FspBI	CTAG	1 cut(s)	326
GlaI	GCGC	3 cut(s)	245, 591, 771
GsuI	CTGGAG	1 cut(s)	236
HaeII	RGCGCY	1 cut(s)	593
HaeIII	GGCC	9 cut(s)	13, 20, 99, 111, 140, 293, 552, 632, 830
HgaI	GACGC	3 cut(s)	209, 433, 644
HhaI	GCGC	3 cut(s)	246, 592, 772
Hin1I	GRCGYC	4 cut(s)	444, 590, 939, 1299
Hin1II	CATG	5 cut(s)	11, 244, 292, 979, 1322
Hin6I	GCGC	3 cut(s)	244, 590, 770
HinP1I	GCGC	3 cut(s)	244, 590, 770
HinfI	GANTC	4 cut(s)	380, 519, 651, 724
HphI	GGTGA	2 cut(s)	443, 1318
Hpy166II	GTNNAC	3 cut(s)	133, 585, 1038
Hpy188I	TCNGA	4 cut(s)	219, 511, 518, 886
Hpy188III	TCNNGA	2 cut(s)	853, 1259
Hpy8I	GTNNAC	3 cut(s)	133, 585, 1038
HpyAV	CCTTC	6 cut(s)	86, 614, 850, 1054, 1066, 1156
HpyCH4III	ACNGT	2 cut(s)	1186, 1330
HpyCH4IV	ACGT	6 cut(s)	459, 741, 939, 1171, 1299, 1310
HpyCH4V	TGCA	1 cut(s)	715
HpyF10VI	GCNNNNNNNGC	5 cut(s)	117, 538, 587, 772, 1049
HpyF3I	CTNAG	2 cut(s)	148, 818
HpySE526I	ACGT	6 cut(s)	459, 741, 939, 1171, 1299, 1310
Hsp92I	GRCGYC	4 cut(s)	444, 590, 939, 1299
Hsp92II	CATG	5 cut(s)	11, 244, 292, 979, 1322
HspAI	GCGC	3 cut(s)	244, 590, 770
KasI	GGCGCC	1 cut(s)	589
KroI	GCCGGC	2 cut(s)	99, 766
KroNI	GCCGGC	2 cut(s)	101, 768
Kzo9I	GATC	5 cut(s)	231, 306, 354, 623, 777
LguI	GCTCTTC	1 cut(s)	350
LmnI	GCTCC	3 cut(s)	211, 535, 1023
Lsp1109I	GCAGC	5 cut(s)	129, 418, 550, 777, 878
LweI	GCATC	1 cut(s)	702
MaeI	CTAG	1 cut(s)	326
MaeII	ACGT	6 cut(s)	459, 741, 939, 1171, 1299, 1310
MaeIII	GTNAC	2 cut(s)	832, 1306
MalI	GATC	5 cut(s)	233, 308, 356, 625, 779
MboI	GATC	5 cut(s)	231, 306, 354, 623, 777
MboII	GAAGA	5 cut(s)	337, 652, 733, 883, 1243
MflI	RGATCY	4 cut(s)	231, 306, 354, 623
MhlI	GDGCHC	2 cut(s)	22, 345
MlsI	TGGCCA	1 cut(s)	293
MluI	ACGCGT	2 cut(s)	636, 957
MluNI	TGGCCA	1 cut(s)	293
Mly113I	GGCGCC	1 cut(s)	590
MlyI	GAGTC	2 cut(s)	513, 660
Mox20I	TGGCCA	1 cut(s)	293
Mph1103I	ATGCAT	1 cut(s)	717
MroNI	GCCGGC	2 cut(s)	99, 766
MroXI	GAANNNNTTC	1 cut(s)	959
MscI	TGGCCA	1 cut(s)	293
MseI	TTAA	2 cut(s)	174, 1338
Msp20I	TGGCCA	1 cut(s)	293
MspA1I	CMGCKG	2 cut(s)	730, 790
MspR9I	CCNGG	5 cut(s)	113, 227, 254, 608, 749
MvaI	CCWGG	1 cut(s)	254
MvnI	CGCG	4 cut(s)	456, 638, 772, 959
MwoI	GCNNNNNNNGC	5 cut(s)	117, 538, 587, 772, 1049
NaeI	GCCGGC	2 cut(s)	101, 768
NarI	GGCGCC	1 cut(s)	590
NciI	CCSGG	4 cut(s)	113, 227, 608, 749
NcoI	CCATGG	1 cut(s)	7
NdeII	GATC	5 cut(s)	231, 306, 354, 623, 777
NgoMIV	GCCGGC	2 cut(s)	99, 766
NheI	GCTAGC	1 cut(s)	325
NlaIII	CATG	5 cut(s)	11, 244, 292, 979, 1322
NlaIV	GGNNCC	6 cut(s)	20, 21, 213, 591, 625, 851
NmeAIII	GCCGAG	1 cut(s)	60
NmuCI	GTSAC	2 cut(s)	832, 1306
NsiI	ATGCAT	1 cut(s)	717
NspV	TTCGAA	1 cut(s)	761
PciSI	GCTCTTC	1 cut(s)	350
PcsI	WCGNNNNNNNCGW	4 cut(s)	162, 441, 453, 842
PdiI	GCCGGC	2 cut(s)	101, 768
PdmI	GAANNNNTTC	1 cut(s)	959
PfeI	GAWTC	2 cut(s)	380, 724
PfoI	TCCNGGA	2 cut(s)	225, 252
PleI	GAGTC	2 cut(s)	513, 659
PluTI	GGCGCC	1 cut(s)	593
PpsI	GAGTC	2 cut(s)	513, 659
Psp124BI	GAGCTC	1 cut(s)	345
Psp6I	CCWGG	1 cut(s)	252
PspGI	CCWGG	1 cut(s)	252
PspN4I	GGNNCC	6 cut(s)	20, 21, 213, 591, 625, 851
PspOMI	GGGCCC	1 cut(s)	18
PspPI	GGNCC	8 cut(s)	11, 18, 19, 97, 109, 139, 256, 642
PsuI	RGATCY	4 cut(s)	231, 306, 354, 623
PvuII	CAGCTG	1 cut(s)	790
RsaI	GTAC	5 cut(s)	89, 584, 605, 617, 1103
RsaNI	GTAC	5 cut(s)	88, 583, 604, 616, 1102
SacI	GAGCTC	1 cut(s)	345
SapI	GCTCTTC	1 cut(s)	350
SaqAI	TTAA	2 cut(s)	174, 1338
Sau3AI	GATC	5 cut(s)	231, 306, 354, 623, 777
Sau96I	GGNCC	8 cut(s)	11, 18, 19, 97, 109, 139, 256, 642
SchI	GAGTC	2 cut(s)	513, 660
ScrFI	CCNGG	5 cut(s)	113, 227, 254, 608, 749
SduI	GDGCHC	2 cut(s)	22, 345
SfaNI	GCATC	1 cut(s)	702
SfoI	GGCGCC	1 cut(s)	591
SfuI	TTCGAA	1 cut(s)	761
SgrAI	CRCCGGYG	1 cut(s)	332
SinI	GGWCC	2 cut(s)	256, 642
SmlI	CTYRAG	1 cut(s)	500
SmoI	CTYRAG	1 cut(s)	500
SspDI	GGCGCC	1 cut(s)	589
SspMI	CTAG	1 cut(s)	326
SstI	GAGCTC	1 cut(s)	345
StyD4I	CCNGG	5 cut(s)	111, 225, 252, 606, 747
StyI	CCWWGG	3 cut(s)	7, 126, 627
TaaI	ACNGT	2 cut(s)	1186, 1330
TaiI	ACGT	6 cut(s)	462, 744, 942, 1174, 1302, 1313
TaqI	TCGA	9 cut(s)	183, 321, 483, 690, 761, 795, 903, 963, 1008
TauI	GCSGC	9 cut(s)	41, 249, 339, 396, 469, 733, 775, 1055, 1208
TfiI	GAWTC	2 cut(s)	380, 724
Tru1I	TTAA	2 cut(s)	174, 1338
Tru9I	TTAA	2 cut(s)	174, 1338
TseFI	GTSAC	2 cut(s)	832, 1306
TseI	GCWGC	5 cut(s)	117, 406, 538, 790, 891
Tsp45I	GTSAC	2 cut(s)	832, 1306
TspDTI	ATGAA	1 cut(s)	968
TspGWI	ACGGA	3 cut(s)	381, 507, 851
VpaK11BI	GGWCC	2 cut(s)	256, 642
XcmI	CCANNNNNNNNNTGG	1 cut(s)	566
XmiI	GTMKAC	1 cut(s)	132
XmnI	GAANNNNTTC	1 cut(s)	959
XspI	CTAG	1 cut(s)	326
ZraI	GACGTC	2 cut(s)	940, 1300
Zsp2I	ATGCAT	1 cut(s)	717

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.