MD16G1224800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012196
Physical Location & Seq
Reverse (-)
22686207 .. 22689905
3699 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1224800.v1.1.491

Sequence Viewer

Length: 300 bp
ATGGATACGGATGCTAAGGATGCCAGTACTGCTAATGGGTGTAAAACTGAGACGAAGTCGGATACCGGAGACTCTCAGAAAGTTGAGGAAAATGTTAATGGAGATGAAGACAGTGAGTCTAATTCTTTGTTGCCTCCTCGGAGAGGGGGCATGTCGAGGAAAACGAATAAGACTCGGCGAAAAGTGCAGTGGAATGATAGGAATGGGAATAAGCTTGTTGAGGTCCTGGAATTCGAACCAAGCGATGTTAGTGATTCTGATGATGAGGATGCAGATTCTTGCATATGTACTATCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

100

Amino Acids

10.92

Weight (kDa)

4.42

Isoelectric Point (pI)

56.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012196)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33390 AT2G33390
fragaria_vesca FvH4_4g04340 FvH4_4g04340
malus_domestica MD13G1219400.v1.1 MD16G1224800.v1.1
prunus_persica Prupe.1G046100_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0394791 RchiOBHm_Chr4g0394821
rosa_laevigata RLG00000009660
rosa_multiflora Rmu_sc0005356.1_g000001
rosa_roxburghii Rroxscaffold_5G00339830
rosa_rugosa Rorug03G0361300.1 Rorug03G0361400.1
rosa_samantha Rh4AG055200 Rh4BG053100 Rh4CG060000 Rh4DG051700
rosa_wichuraiana Rw4G004410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 230
AfaI GTAC 2 cut(s) 28, 289
AfiI CCNNNNNNNGG 1 cut(s) 143
AhdI GACNNNNNGTC 1 cut(s) 115
AjnI CCWGG 1 cut(s) 225
AluBI AGCT 1 cut(s) 214
AluI AGCT 1 cut(s) 214
Alw26I GTCTC 2 cut(s) 44, 63
ApoI RAATTY 1 cut(s) 230
AspS9I GGNCC 1 cut(s) 223
AsuII TTCGAA 1 cut(s) 234
AvaII GGWCC 1 cut(s) 223
BbsI GAAGAC 1 cut(s) 114
BciT130I CCWGG 1 cut(s) 227
BciVI GTATCC 1 cut(s) 55
BcoDI GTCTC 2 cut(s) 44, 63
BfuI GTATCC 1 cut(s) 55
BmcAI AGTACT 1 cut(s) 28
Bme1390I CCNGG 1 cut(s) 227
Bme18I GGWCC 1 cut(s) 223
BmeRI GACNNNNNGTC 1 cut(s) 115
BmgT120I GGNCC 1 cut(s) 223
BmrFI CCNGG 1 cut(s) 227
BmsI GCATC 2 cut(s) 10, 259
BpiI GAAGAC 1 cut(s) 114
Bpu10I CCTNAGC 1 cut(s) 15
Bpu14I TTCGAA 1 cut(s) 234
BsaJI CCNNGG 1 cut(s) 137
BsaWI WCCGGW 1 cut(s) 65
Bsc4I CCNNNNNNNGG 1 cut(s) 143
Bse1I ACTGG 1 cut(s) 24
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 1 cut(s) 137
BseGI GGATG 3 cut(s) 16, 25, 274
BseLI CCNNNNNNNGG 1 cut(s) 143
BseMII CTCAG 2 cut(s) 39, 89
BseNI ACTGG 1 cut(s) 24
BseRI GAGGAG 1 cut(s) 126
BsgI GTGCAG 1 cut(s) 206
BsiSI CCGG 1 cut(s) 66
BslI CCNNNNNNNGG 1 cut(s) 143
BsmAI GTCTC 2 cut(s) 44, 63
BsmBI CGTCTC 1 cut(s) 44
Bsp119I TTCGAA 1 cut(s) 234
BspCNI CTCAG 2 cut(s) 40, 88
BspT104I TTCGAA 1 cut(s) 234
BsrI ACTGG 1 cut(s) 24
BssECI CCNNGG 1 cut(s) 137
Bst2UI CCWGG 1 cut(s) 227
Bst4CI ACNGT 1 cut(s) 113
BstBI TTCGAA 1 cut(s) 234
BstDEI CTNAG 3 cut(s) 15, 48, 75
BstENI CCTNNNNNAGG 1 cut(s) 141
BstF5I GGATG 3 cut(s) 16, 25, 274
BstMAI GTCTC 2 cut(s) 44, 63
BstMWI GCNNNNNNNGC 3 cut(s) 20, 29, 184
BstNI CCWGG 1 cut(s) 227
BstNSI RCATGY 1 cut(s) 154
BstSCI CCNGG 1 cut(s) 225
BstV2I GAAGAC 1 cut(s) 114
BsuI GTATCC 1 cut(s) 55
BtgZI GCGATG 1 cut(s) 258
BtsCI GGATG 3 cut(s) 16, 25, 274
BtsI GCAGTG 1 cut(s) 194
BtsIMutI CAGTG 2 cut(s) 118, 194
Cfr13I GGNCC 1 cut(s) 223
Csp6I GTAC 2 cut(s) 27, 288
CviAII CATG 2 cut(s) 151, 295
CviJI RGCY 1 cut(s) 214
CviKI_1 RGCY 1 cut(s) 214
CviQI GTAC 2 cut(s) 27, 288
DdeI CTNAG 3 cut(s) 15, 48, 75
DriI GACNNNNNGTC 1 cut(s) 115
Eam1105I GACNNNNNGTC 1 cut(s) 115
Eco47I GGWCC 1 cut(s) 223
EcoNI CCTNNNNNAGG 1 cut(s) 141
EcoO109I RGGNCCY 1 cut(s) 223
EcoRI GAATTC 1 cut(s) 230
EcoRII CCWGG 1 cut(s) 225
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 2 cut(s) 154, 298
FaiI YATR 4 cut(s) 152, 284, 286, 296
FatI CATG 2 cut(s) 150, 294
FauNDI CATATG 1 cut(s) 284
FokI GGATG 3 cut(s) 23, 32, 281
HapII CCGG 1 cut(s) 66
Hin1II CATG 2 cut(s) 154, 298
HindIII AAGCTT 1 cut(s) 212
HinfI GANTC 5 cut(s) 71, 116, 172, 254, 275
HpaII CCGG 1 cut(s) 66
Hpy188I TCNGA 4 cut(s) 61, 78, 141, 259
HpyCH4III ACNGT 1 cut(s) 113
HpyCH4V TGCA 3 cut(s) 187, 272, 282
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 29, 184
HpyF3I CTNAG 3 cut(s) 15, 48, 75
Hsp92II CATG 2 cut(s) 154, 298
LpnPI CCDG 4 cut(s) 37, 79, 212, 239
LweI GCATC 2 cut(s) 10, 259
MboII GAAGA 1 cut(s) 119
MluCI AATT 2 cut(s) 121, 230
MlyI GAGTC 3 cut(s) 65, 125, 166
MmeI TCCRAC 1 cut(s) 39
MnlI CCTC 7 cut(s) 79, 137, 144, 147, 150, 214, 259
MseI TTAA 1 cut(s) 96
MspI CCGG 1 cut(s) 66
MspR9I CCNGG 1 cut(s) 227
MvaI CCWGG 1 cut(s) 227
MwoI GCNNNNNNNGC 3 cut(s) 20, 29, 184
NdeI CATATG 1 cut(s) 284
NlaIII CATG 2 cut(s) 154, 298
NmeAIII GCCGAG 1 cut(s) 154
NspI RCATGY 1 cut(s) 154
NspV TTCGAA 1 cut(s) 234
PcsI WCGNNNNNNNCGW 2 cut(s) 161, 240
PfeI GAWTC 2 cut(s) 254, 275
PflFI GACNNNGTC 1 cut(s) 55
PfoI TCCNGGA 1 cut(s) 225
PleI GAGTC 3 cut(s) 65, 124, 166
PpsI GAGTC 3 cut(s) 65, 124, 166
PpuMI RGGWCCY 1 cut(s) 223
Psp5II RGGWCCY 1 cut(s) 223
Psp6I CCWGG 1 cut(s) 225
PspGI CCWGG 1 cut(s) 225
PspPI GGNCC 1 cut(s) 223
PspPPI RGGWCCY 1 cut(s) 223
PsyI GACNNNGTC 1 cut(s) 55
RsaI GTAC 2 cut(s) 28, 289
RsaNI GTAC 2 cut(s) 27, 288
SaqAI TTAA 1 cut(s) 96
Sau96I GGNCC 1 cut(s) 223
ScaI AGTACT 1 cut(s) 28
SchI GAGTC 3 cut(s) 65, 125, 166
ScrFI CCNGG 1 cut(s) 227
SetI ASST 2 cut(s) 216, 225
SfaNI GCATC 2 cut(s) 10, 259
SfuI TTCGAA 1 cut(s) 234
SinI GGWCC 1 cut(s) 223
Sse9I AATT 2 cut(s) 121, 230
StyD4I CCNGG 1 cut(s) 225
TaaI ACNGT 1 cut(s) 113
TaqI TCGA 2 cut(s) 155, 234
TasI AATT 2 cut(s) 121, 230
TatI WGTACW 2 cut(s) 26, 287
TfiI GAWTC 2 cut(s) 254, 275
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TscAI CASTG 2 cut(s) 118, 194
TspDTI ATGAA 1 cut(s) 120
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 2 cut(s) 118, 194
Tth111I GACNNNGTC 1 cut(s) 55
VpaK11BI GGWCC 1 cut(s) 223
XagI CCTNNNNNAGG 1 cut(s) 141
XapI RAATTY 1 cut(s) 230
XceI RCATGY 1 cut(s) 154
ZrmI AGTACT 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.