MD16G1234100.v1.1

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012901
Physical Location & Seq
Forward (+)
24063452 .. 24064003
552 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1234100.v1.1.491

Sequence Viewer

Length: 552 bp
ATGACGTTATCATTCCAAGCTCGTGAAAAAGATTTTTTTTGTTTGAGGCCACCTTTAAAAGAAAGGCTTGTATTTGGCCTGTATTTCATTCGATCATTTCCACCATACCTTCACACTCAAAACATGAAGATCCTTACCAGCATTTTGCTTATTTTTTCTCTTGCATTCTTCGTAATCTGCATGTCAATACTAGAACCCCAGAACTTCTACGGCACCGAATACAACATTCGAGTCGTCAACGGCTTCACAAACAACTCGTCCTTGCCGTTGGTGATTTGGTGTGCATCACAGAACAGTGATCTTGGTGGACGTGCGCTTCAGGAGCATGACGATTTTAGCTGGAGCCTGAGGACCAGTCTTTGGGGCACTACTGACCTTTTCAAATGTACCATGAAATGGGACCGAATAAGGAGGAGTTTTGAGGCGTTTAAGGCTTCGAGGGATATTCAGAGGTGCGGTCCTTTTAGGAAGTGTTCTTGGTTGGTCAGAGAAGATGGGTTTTATTTCAGCAATGATGAAGTAAATTGGAAAAAAGATTTTTCATGGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

184

Amino Acids

21.71

Weight (kDa)

8.95

Isoelectric Point (pI)

45.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 76 - 182 3.1e-25 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012901)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24060
fragaria_vesca FvH4_4g05670
malus_domestica MD13G1228600.v1.1 MD16G1234100.v1.1
prunus_persica Prupe.1G058400_v2.0.a1
pyrus_communis pycom13g20090
rosa_chinensis RchiOBHm_Chr4g0396841
rosa_laevigata RLG00000009500
rosa_multiflora Rmu_sc0000487.1_g000063
rosa_roxburghii Rroxscaffold_5G00341700
rosa_rugosa Rorug03G0347300
rosa_samantha Rh4AG072200 Rh4AG073000 Rh4AG074600 Rh4BG070200 Rh4CG078600 Rh4DG067900
rosa_wichuraiana Rw4G005950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 212
AccB7I CCANNNNNTGG 2 cut(s) 360, 396
AciI CCGC 1 cut(s) 456
AclWI GGATC 1 cut(s) 124
AcuI CTGAAG 1 cut(s) 302
AfaI GTAC 1 cut(s) 388
AfiI CCNNNNNNNGG 2 cut(s) 360, 396
AgsI TTSAA 1 cut(s) 382
AjiI CACGTC 1 cut(s) 311
AluBI AGCT 2 cut(s) 20, 339
AluI AGCT 2 cut(s) 20, 339
AlwI GGATC 1 cut(s) 124
AoxI GGCC 2 cut(s) 47, 76
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AspLEI GCGC 1 cut(s) 316
AspS9I GGNCC 3 cut(s) 351, 400, 458
AsuHPI GGTGA 1 cut(s) 283
AvaII GGWCC 3 cut(s) 351, 400, 458
AxyI CCTNAGG 1 cut(s) 347
BaeGI GKGCMC 1 cut(s) 368
BanI GGYRCC 1 cut(s) 212
BauI CACGAG 1 cut(s) 21
BccI CCATC 1 cut(s) 488
BceAI ACGGC 3 cut(s) 226, 250, 256
BfaI CTAG 1 cut(s) 191
Bme18I GGWCC 3 cut(s) 351, 400, 458
BmgBI CACGTC 1 cut(s) 311
BmgT120I GGNCC 3 cut(s) 351, 400, 458
BmiI GGNNCC 3 cut(s) 214, 344, 401
BmsI GCATC 1 cut(s) 293
BpmI CTGGAG 1 cut(s) 361
Bsc4I CCNNNNNNNGG 2 cut(s) 360, 396
Bse1I ACTGG 1 cut(s) 354
Bse21I CCTNAGG 1 cut(s) 347
Bse3DI GCAATG 1 cut(s) 517
BseLI CCNNNNNNNGG 2 cut(s) 360, 396
BseMI GCAATG 1 cut(s) 517
BseMII CTCAG 1 cut(s) 338
BseNI ACTGG 1 cut(s) 354
BseRI GAGGAG 1 cut(s) 427
BseSI GKGCMC 1 cut(s) 368
BshFI GGCC 2 cut(s) 49, 78
BshNI GGYRCC 1 cut(s) 212
BslFI GGGAC 1 cut(s) 413
BslI CCNNNNNNNGG 2 cut(s) 360, 396
BsmFI GGGAC 1 cut(s) 413
BsmI GAATGC 1 cut(s) 164
BsnI GGCC 2 cut(s) 49, 78
Bsp1286I GDGCHC 1 cut(s) 368
Bsp143I GATC 3 cut(s) 92, 129, 298
BspACI CCGC 1 cut(s) 456
BspANI GGCC 2 cut(s) 49, 78
BspCNI CTCAG 1 cut(s) 339
BspLI GGNNCC 3 cut(s) 214, 344, 401
BspPI GGATC 1 cut(s) 124
BspT107I GGYRCC 1 cut(s) 212
BsrDI GCAATG 1 cut(s) 517
BsrI ACTGG 1 cut(s) 354
BssMI GATC 3 cut(s) 92, 129, 298
BssSI CACGAG 1 cut(s) 21
Bst2BI CACGAG 1 cut(s) 21
Bst4CI ACNGT 1 cut(s) 296
BstDEI CTNAG 1 cut(s) 347
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 3 cut(s) 95, 132, 301
BstMBI GATC 3 cut(s) 92, 129, 298
BstMWI GCNNNNNNNGC 2 cut(s) 322, 431
BstNSI RCATGY 1 cut(s) 184
BstSLI GKGCMC 1 cut(s) 368
BstX2I RGATCY 1 cut(s) 129
BstYI RGATCY 1 cut(s) 129
Bsu36I CCTNAGG 1 cut(s) 347
BsuRI GGCC 2 cut(s) 49, 78
BtrI CACGTC 1 cut(s) 311
BtsIMutI CAGTG 1 cut(s) 301
CfoI GCGC 1 cut(s) 316
Cfr13I GGNCC 3 cut(s) 351, 400, 458
Csp6I GTAC 1 cut(s) 387
CviAII CATG 5 cut(s) 124, 181, 326, 391, 543
CviJI RGCY 8 cut(s) 20, 49, 67, 78, 243, 339, 345, 434
CviKI_1 RGCY 8 cut(s) 20, 49, 67, 78, 243, 339, 345, 434
CviQI GTAC 1 cut(s) 387
DdeI CTNAG 1 cut(s) 347
DpnI GATC 3 cut(s) 94, 131, 300
DpnII GATC 3 cut(s) 92, 129, 298
DraI TTTAAA 1 cut(s) 57
Eco47I GGWCC 3 cut(s) 351, 400, 458
Eco57I CTGAAG 1 cut(s) 302
Eco81I CCTNAGG 1 cut(s) 347
FaeI CATG 5 cut(s) 127, 184, 329, 394, 546
FaiI YATR 6 cut(s) 106, 125, 182, 327, 392, 544
FalI AAGNNNNNCTT 2 cut(s) 51, 83
FaqI GGGAC 1 cut(s) 413
FatI CATG 5 cut(s) 123, 180, 325, 390, 542
FspBI CTAG 1 cut(s) 191
GlaI GCGC 1 cut(s) 315
GsuI CTGGAG 1 cut(s) 361
HaeIII GGCC 2 cut(s) 49, 78
HhaI GCGC 1 cut(s) 316
Hin1II CATG 5 cut(s) 127, 184, 329, 394, 546
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HinfI GANTC 1 cut(s) 231
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 2 cut(s) 238, 308
Hpy188I TCNGA 2 cut(s) 450, 488
Hpy188III TCNNGA 2 cut(s) 23, 320
Hpy8I GTNNAC 2 cut(s) 238, 308
HpyAV CCTTC 1 cut(s) 119
HpyCH4III ACNGT 1 cut(s) 296
HpyCH4IV ACGT 2 cut(s) 5, 310
HpyCH4V TGCA 3 cut(s) 164, 180, 284
HpyF10VI GCNNNNNNNGC 2 cut(s) 322, 431
HpyF3I CTNAG 1 cut(s) 347
HpySE526I ACGT 2 cut(s) 5, 310
Hsp92II CATG 5 cut(s) 127, 184, 329, 394, 546
HspAI GCGC 1 cut(s) 314
Kzo9I GATC 3 cut(s) 92, 129, 298
LmnI GCTCC 2 cut(s) 322, 342
LpnPI CCDG 7 cut(s) 92, 151, 212, 305, 325, 359, 367
LweI GCATC 1 cut(s) 293
MaeI CTAG 1 cut(s) 191
MaeII ACGT 2 cut(s) 5, 310
MalI GATC 3 cut(s) 94, 131, 300
MboI GATC 3 cut(s) 92, 129, 298
MboII GAAGA 3 cut(s) 139, 160, 503
MflI RGATCY 1 cut(s) 129
MhlI GDGCHC 1 cut(s) 368
MluCI AATT 1 cut(s) 523
MlyI GAGTC 1 cut(s) 240
MnlI CCTC 6 cut(s) 39, 342, 405, 415, 432, 444
MseI TTAA 2 cut(s) 56, 429
Mva1269I GAATGC 1 cut(s) 164
MwoI GCNNNNNNNGC 2 cut(s) 322, 431
NdeII GATC 3 cut(s) 92, 129, 298
NlaIII CATG 5 cut(s) 127, 184, 329, 394, 546
NlaIV GGNNCC 3 cut(s) 214, 344, 401
NspI RCATGY 1 cut(s) 184
PcsI WCGNNNNNNNCGW 1 cut(s) 263
PctI GAATGC 1 cut(s) 164
PflMI CCANNNNNTGG 2 cut(s) 360, 396
PleI GAGTC 1 cut(s) 239
PpsI GAGTC 1 cut(s) 239
PspN4I GGNNCC 3 cut(s) 214, 344, 401
PspPI GGNCC 3 cut(s) 351, 400, 458
PsuI RGATCY 1 cut(s) 129
RsaI GTAC 1 cut(s) 388
RsaNI GTAC 1 cut(s) 387
SaqAI TTAA 2 cut(s) 56, 429
Sau3AI GATC 3 cut(s) 92, 129, 298
Sau96I GGNCC 3 cut(s) 351, 400, 458
SchI GAGTC 1 cut(s) 240
SduI GDGCHC 1 cut(s) 368
SetI ASST 8 cut(s) 8, 22, 55, 111, 313, 341, 378, 455
SfaNI GCATC 1 cut(s) 293
SinI GGWCC 3 cut(s) 351, 400, 458
Sse9I AATT 1 cut(s) 523
SsiI CCGC 1 cut(s) 456
SspMI CTAG 1 cut(s) 191
TaaI ACNGT 1 cut(s) 296
TaiI ACGT 2 cut(s) 8, 313
TaqI TCGA 3 cut(s) 91, 229, 437
TaqII GACCGA 1 cut(s) 417
TasI AATT 1 cut(s) 523
Tru1I TTAA 2 cut(s) 56, 429
Tru9I TTAA 2 cut(s) 56, 429
TscAI CASTG 1 cut(s) 301
TspDTI ATGAA 5 cut(s) 76, 140, 407, 531, 531
TspRI CASTG 1 cut(s) 301
Van91I CCANNNNNTGG 2 cut(s) 360, 396
VpaK11BI GGWCC 3 cut(s) 351, 400, 458
XceI RCATGY 1 cut(s) 184
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.