Rh4AG074600

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0012901
Physical Location & Seq
Forward (+)
15266018 .. 15285402
19385 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG074600.1

Sequence Viewer

Length: 570 bp
ATGAAAATCATCAACAGCGTTCTCCTCATATGTGGCCTCGCATCCTTCGTGATCTTTATGTCTCTGTTGGAACCCCAGAACTTGCTGGGCACTGAATATGACGTTCGAGTCATCAACGGCTTCACAAACAACTCGTCCCTGCCGCTGGTAATCTGGTGTTCATCGAAAGAGAATGATCTCGGTGGACGTGCGCTACAGGAGAAGGATGACTTCAGTTGGAGGCTGAGGACCAACTTCTGGGGCACAAGTAACCATTTCGTTTGCACGATGAAATGGGACAAAAAGCGGAAGCGCTTCGACGCCTTCAAGATTCCGAGAGACGTTCAACGGTGTGGTCCTTTCCGGAAGTGCTCATGGCTGGTGAGGGAGGATGGGTTTTATTTCAGCAACGATGAAGTTTGTCCTAATGCTCTTGAACCACTTTTCAAACAAATGGTGAACAAATTTATAGATGATCATGCACAGCCAAAGCTTTCGATGGTTTTGTCTCCATATATGGGTGTTGTCGTTGTCGTTAGTGTGGTTCAAAAGATTCCTTATTTGTTTATCAAGGTCAAGGTCAAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

189

Amino Acids

21.88

Weight (kDa)

9.15

Isoelectric Point (pI)

32.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 35 - 132 4.4e-24 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012901)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24060
fragaria_vesca FvH4_4g05670
malus_domestica MD13G1228600.v1.1 MD16G1234100.v1.1
prunus_persica Prupe.1G058400_v2.0.a1
pyrus_communis pycom13g20090
rosa_chinensis RchiOBHm_Chr4g0396841
rosa_laevigata RLG00000009500
rosa_multiflora Rmu_sc0000487.1_g000063
rosa_roxburghii Rroxscaffold_5G00341700
rosa_rugosa Rorug03G0347300
rosa_samantha Rh4AG072200 Rh4AG073000 Rh4AG074600 Rh4BG070200 Rh4CG078600 Rh4DG067900
rosa_wichuraiana Rw4G005950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 107
AccB7I CCANNNNNTGG 1 cut(s) 237
AccIII TCCGGA 1 cut(s) 342
AciI CCGC 2 cut(s) 143, 286
AcsI RAATTY 1 cut(s) 443
AcuI CTGAAG 1 cut(s) 196
AcyI GRCGYC 1 cut(s) 300
AfeI AGCGCT 1 cut(s) 293
AfiI CCNNNNNNNGG 3 cut(s) 145, 237, 497
AgsI TTSAA 5 cut(s) 307, 326, 416, 427, 527
AjiI CACGTC 1 cut(s) 188
AluBI AGCT 1 cut(s) 472
AluI AGCT 1 cut(s) 472
Alw21I GWGCWC 1 cut(s) 353
Alw26I GTCTC 3 cut(s) 66, 312, 492
Aor13HI TCCGGA 1 cut(s) 342
Aor51HI AGCGCT 1 cut(s) 293
AoxI GGCC 1 cut(s) 34
ApoI RAATTY 1 cut(s) 443
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
Asp700I GAANNNNTTC 1 cut(s) 293
AspLEI GCGC 2 cut(s) 193, 294
AspS9I GGNCC 2 cut(s) 228, 335
AsuHPI GGTGA 2 cut(s) 373, 448
AvaII GGWCC 2 cut(s) 228, 335
BaeGI GKGCMC 2 cut(s) 92, 245
Bbv12I GWGCWC 1 cut(s) 353
BbvCI CCTCAGC 1 cut(s) 224
BccI CCATC 2 cut(s) 365, 472
BceAI ACGGC 1 cut(s) 133
BclI TGATCA 1 cut(s) 454
BcoDI GTCTC 3 cut(s) 66, 312, 492
BfmI CTRYAG 1 cut(s) 194
BfoI RGCGCY 1 cut(s) 295
BisI GCNGC 1 cut(s) 143
BlsI GCNGC 1 cut(s) 144
Bme18I GGWCC 2 cut(s) 228, 335
BmgBI CACGTC 1 cut(s) 188
BmgT120I GGNCC 2 cut(s) 228, 335
BmiI GGNNCC 1 cut(s) 72
BmsI GCATC 1 cut(s) 50
Bpu10I CCTNAGC 1 cut(s) 224
BsaHI GRCGYC 1 cut(s) 300
BsaWI WCCGGW 1 cut(s) 342
BsaXI ACNNNNNCTCC 2 cut(s) 359, 389
Bsc4I CCNNNNNNNGG 3 cut(s) 145, 237, 497
BseAI TCCGGA 1 cut(s) 342
BseGI GGATG 3 cut(s) 41, 211, 376
BseLI CCNNNNNNNGG 3 cut(s) 145, 237, 497
BseMII CTCAG 1 cut(s) 215
BseRI GAGGAG 1 cut(s) 14
BseSI GKGCMC 2 cut(s) 92, 245
BseYI CCCAGC 1 cut(s) 85
BshFI GGCC 1 cut(s) 36
BsiHKAI GWGCWC 1 cut(s) 353
BsiSI CCGG 1 cut(s) 343
BslFI GGGAC 2 cut(s) 121, 290
BslI CCNNNNNNNGG 3 cut(s) 145, 237, 497
BsmAI GTCTC 3 cut(s) 66, 312, 492
BsmBI CGTCTC 1 cut(s) 312
BsmFI GGGAC 2 cut(s) 121, 290
BsnI GGCC 1 cut(s) 36
Bsp1286I GDGCHC 3 cut(s) 92, 245, 353
Bsp13I TCCGGA 1 cut(s) 342
Bsp143I GATC 3 cut(s) 51, 175, 454
BspACI CCGC 2 cut(s) 143, 286
BspANI GGCC 1 cut(s) 36
BspCNI CTCAG 1 cut(s) 216
BspEI TCCGGA 1 cut(s) 342
BspLI GGNNCC 1 cut(s) 72
BssMI GATC 3 cut(s) 51, 175, 454
BssNI GRCGYC 1 cut(s) 300
Bst4CI ACNGT 1 cut(s) 330
BstACI GRCGYC 1 cut(s) 300
BstDEI CTNAG 1 cut(s) 224
BstF5I GGATG 3 cut(s) 41, 211, 376
BstH2I RGCGCY 1 cut(s) 295
BstHHI GCGC 2 cut(s) 193, 294
BstKTI GATC 3 cut(s) 54, 178, 457
BstMAI GTCTC 3 cut(s) 66, 312, 492
BstMBI GATC 3 cut(s) 51, 175, 454
BstSFI CTRYAG 1 cut(s) 194
BstSLI GKGCMC 2 cut(s) 92, 245
BsuRI GGCC 1 cut(s) 36
BtrI CACGTC 1 cut(s) 188
BtsCI GGATG 3 cut(s) 41, 211, 376
BtsIMutI CAGTG 1 cut(s) 90
CfoI GCGC 2 cut(s) 193, 294
Cfr13I GGNCC 2 cut(s) 228, 335
CseI GACGC 1 cut(s) 308
CviAII CATG 2 cut(s) 354, 458
CviJI RGCY 6 cut(s) 36, 120, 223, 358, 466, 472
CviKI_1 RGCY 6 cut(s) 36, 120, 223, 358, 466, 472
DdeI CTNAG 1 cut(s) 224
DpnI GATC 3 cut(s) 53, 177, 456
DpnII GATC 3 cut(s) 51, 175, 454
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
Eco47I GGWCC 2 cut(s) 228, 335
Eco47III AGCGCT 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 196
Esp3I CGTCTC 1 cut(s) 312
FaeI CATG 2 cut(s) 357, 461
FalI AAGNNNNNCTT 2 cut(s) 194, 226
FaqI GGGAC 2 cut(s) 121, 290
FatI CATG 2 cut(s) 353, 457
FauNDI CATATG 1 cut(s) 29
FbaI TGATCA 1 cut(s) 454
Fnu4HI GCNGC 1 cut(s) 143
FokI GGATG 3 cut(s) 28, 218, 383
Fsp4HI GCNGC 1 cut(s) 143
GlaI GCGC 2 cut(s) 192, 293
GluI GCNGC 1 cut(s) 143
GsaI CCCAGC 1 cut(s) 89
HaeII RGCGCY 1 cut(s) 295
HaeIII GGCC 1 cut(s) 36
HapII CCGG 1 cut(s) 343
HgaI GACGC 1 cut(s) 308
HhaI GCGC 2 cut(s) 193, 294
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 2 cut(s) 357, 461
Hin6I GCGC 2 cut(s) 191, 292
HinP1I GCGC 2 cut(s) 191, 292
HindIII AAGCTT 1 cut(s) 470
HinfI GANTC 3 cut(s) 108, 310, 532
HpaII CCGG 1 cut(s) 343
HphI GGTGA 2 cut(s) 373, 448
Hpy166II GTNNAC 2 cut(s) 185, 439
Hpy188I TCNGA 1 cut(s) 315
Hpy188III TCNNGA 4 cut(s) 49, 307, 343, 413
Hpy8I GTNNAC 2 cut(s) 185, 439
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 3 cut(s) 55, 196, 313
HpyCH4III ACNGT 1 cut(s) 330
HpyCH4IV ACGT 3 cut(s) 102, 187, 321
HpyCH4V TGCA 2 cut(s) 264, 461
HpyF3I CTNAG 1 cut(s) 224
HpySE526I ACGT 3 cut(s) 102, 187, 321
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 2 cut(s) 357, 461
HspAI GCGC 2 cut(s) 191, 292
Kpn2I TCCGGA 1 cut(s) 342
Ksp22I TGATCA 1 cut(s) 454
Kzo9I GATC 3 cut(s) 51, 175, 454
LpnPI CCDG 9 cut(s) 71, 89, 131, 139, 152, 182, 223, 344, 356
LweI GCATC 1 cut(s) 50
MaeII ACGT 3 cut(s) 102, 187, 321
MaeIII GTNAC 1 cut(s) 248
MalI GATC 3 cut(s) 53, 177, 456
MboI GATC 3 cut(s) 51, 175, 454
MhlI GDGCHC 3 cut(s) 92, 245, 353
MluCI AATT 1 cut(s) 443
MlyI GAGTC 1 cut(s) 117
MmeI TCCRAC 2 cut(s) 48, 197
MnlI CCTC 6 cut(s) 35, 47, 213, 219, 357, 361
MroI TCCGGA 1 cut(s) 342
MroXI GAANNNNTTC 1 cut(s) 293
MspA1I CMGCKG 1 cut(s) 145
MspI CCGG 1 cut(s) 343
NdeI CATATG 1 cut(s) 29
NdeII GATC 3 cut(s) 51, 175, 454
NlaIII CATG 2 cut(s) 357, 461
NlaIV GGNNCC 1 cut(s) 72
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PdmI GAANNNNTTC 1 cut(s) 293
PfeI GAWTC 2 cut(s) 310, 532
PflMI CCANNNNNTGG 1 cut(s) 237
PkrI GCNGC 1 cut(s) 144
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
PspFI CCCAGC 1 cut(s) 85
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 2 cut(s) 228, 335
SatI GCNGC 1 cut(s) 143
Sau3AI GATC 3 cut(s) 51, 175, 454
Sau96I GGNCC 2 cut(s) 228, 335
SchI GAGTC 1 cut(s) 117
SduI GDGCHC 3 cut(s) 92, 245, 353
SetI ASST 6 cut(s) 105, 190, 324, 474, 555, 561
SfaNI GCATC 1 cut(s) 50
SfcI CTRYAG 1 cut(s) 194
SinI GGWCC 2 cut(s) 228, 335
Sse9I AATT 1 cut(s) 443
SsiI CCGC 2 cut(s) 143, 286
TaaI ACNGT 1 cut(s) 330
TaiI ACGT 3 cut(s) 105, 190, 324
TaqI TCGA 4 cut(s) 106, 164, 297, 476
TasI AATT 1 cut(s) 443
TauI GCSGC 1 cut(s) 145
TfiI GAWTC 2 cut(s) 310, 532
TscAI CASTG 1 cut(s) 97
TspDTI ATGAA 4 cut(s) 17, 150, 284, 408
TspRI CASTG 1 cut(s) 97
Van91I CCANNNNNTGG 1 cut(s) 237
VpaK11BI GGWCC 2 cut(s) 228, 335
XapI RAATTY 1 cut(s) 443
XmnI GAANNNNTTC 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.