MD16G1254400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0017134
Physical Location & Seq
Reverse (-)
29106281 .. 29106643
363 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1254400.v1.1.491

Sequence Viewer

Length: 363 bp
ATGGGGATTGATCCAAGATCTGGCCTATCGTGCTTGGGAAAGGTGAATGTTGAATACGAGAATGATCAGGATGTGATGATTCAGTTTTATAAATTCATTGCAAGGGAGGAGATGGCATGTGACGAAGCTGAGCTTGGAGCAGAGAAATTTGCTGAAAAGATGCACAGTAAAAAACAATTACAGGAACTGGAGATGCTAAAGCACATGCGTAAATTTCCCAAGGATGATCAATCTGCAATCCTCGAGAAGTTACATCAGCAGATGGAAAACGCCAACTTTGATGGTGCTGCATCTGTTTTGTCGCTGGAGCAGATTCAGGAGATTATTCGAAGGAGGGTGTCTCTTTTATTTAACCTGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

121

Amino Acids

13.96

Weight (kDa)

5.42

Isoelectric Point (pI)

61.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017134)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27050
fragaria_vesca FvH4_6g53561
malus_domestica MD09G1010400.v1.1 MD16G1254400.v1.1
prunus_persica Prupe.3G306700_v2.0.a1
pyrus_communis pycom111g00830
rosa_chinensis RchiOBHm_Chr2g0175601
rosa_laevigata RLG00000022343
rosa_multiflora Rmu_sc0000998.1_g000022 Rmu_sc0012222.1_g000001
rosa_rugosa Rorug02G0586600
rosa_samantha Rh2CG639800
rosa_wichuraiana Rw2G054590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 90
AccB7I CCANNNNNTGG 1 cut(s) 20
AclWI GGATC 1 cut(s) 5
AcsI RAATTY 3 cut(s) 92, 146, 212
AfiI CCNNNNNNNGG 1 cut(s) 20
AgsI TTSAA 1 cut(s) 53
AjuI GAANNNNNNNTTGG 2 cut(s) 117, 149
AluBI AGCT 2 cut(s) 128, 133
AluI AGCT 2 cut(s) 128, 133
Alw26I GTCTC 1 cut(s) 345
AlwI GGATC 1 cut(s) 5
AlwNI CAGNNNCTG 1 cut(s) 187
Ama87I CYCGRG 1 cut(s) 242
AoxI GGCC 1 cut(s) 22
ApeKI GCWGC 1 cut(s) 287
ApoI RAATTY 3 cut(s) 92, 146, 212
AsuHPI GGTGA 1 cut(s) 55
AsuII TTCGAA 1 cut(s) 328
AvaI CYCGRG 1 cut(s) 242
BbvI GCAGC 1 cut(s) 274
BccI CCATC 3 cut(s) 106, 256, 275
BclI TGATCA 2 cut(s) 64, 226
BcoDI GTCTC 1 cut(s) 345
BglII AGATCT 1 cut(s) 17
BisI GCNGC 1 cut(s) 288
BlpI GCTNAGC 1 cut(s) 129
BlsI GCNGC 1 cut(s) 289
BmeT110I CYCGRG 1 cut(s) 242
BmsI GCATC 3 cut(s) 150, 183, 299
BplI GAGNNNNNCTC 2 cut(s) 325, 357
BpmI CTGGAG 2 cut(s) 209, 326
Bpu1102I GCTNAGC 1 cut(s) 129
Bpu14I TTCGAA 1 cut(s) 328
BsaJI CCNNGG 1 cut(s) 219
Bsc4I CCNNNNNNNGG 1 cut(s) 20
Bse1I ACTGG 1 cut(s) 192
Bse3DI GCAATG 1 cut(s) 96
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 2 cut(s) 76, 229
BseLI CCNNNNNNNGG 1 cut(s) 20
BseMI GCAATG 1 cut(s) 96
BseMII CTCAG 2 cut(s) 120, 347
BseNI ACTGG 1 cut(s) 192
BseRI GAGGAG 1 cut(s) 122
BseXI GCAGC 1 cut(s) 274
BshFI GGCC 1 cut(s) 24
BsiHKCI CYCGRG 1 cut(s) 242
BslI CCNNNNNNNGG 1 cut(s) 20
BsmAI GTCTC 1 cut(s) 345
BsnI GGCC 1 cut(s) 24
BsoBI CYCGRG 1 cut(s) 242
Bsp119I TTCGAA 1 cut(s) 328
Bsp143I GATC 4 cut(s) 10, 17, 64, 226
Bsp1720I GCTNAGC 1 cut(s) 129
BspANI GGCC 1 cut(s) 24
BspCNI CTCAG 2 cut(s) 121, 348
BspPI GGATC 1 cut(s) 5
BspT104I TTCGAA 1 cut(s) 328
BsrDI GCAATG 1 cut(s) 96
BsrI ACTGG 1 cut(s) 192
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 4 cut(s) 10, 17, 64, 226
BssT1I CCWWGG 1 cut(s) 219
Bst4CI ACNGT 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 328
BstDEI CTNAG 2 cut(s) 129, 356
BstF5I GGATG 2 cut(s) 76, 229
BstKTI GATC 4 cut(s) 13, 20, 67, 229
BstMAI GTCTC 1 cut(s) 345
BstMBI GATC 4 cut(s) 10, 17, 64, 226
BstMWI GCNNNNNNNGC 1 cut(s) 30
BstNSI RCATGY 2 cut(s) 120, 208
BstV1I GCAGC 1 cut(s) 274
BstX2I RGATCY 1 cut(s) 17
BstYI RGATCY 1 cut(s) 17
BsuRI GGCC 1 cut(s) 24
BtsCI GGATG 2 cut(s) 76, 229
CaiI CAGNNNCTG 1 cut(s) 187
CviAII CATG 2 cut(s) 117, 205
CviJI RGCY 3 cut(s) 24, 128, 133
CviKI_1 RGCY 3 cut(s) 24, 128, 133
DdeI CTNAG 2 cut(s) 129, 356
DpnI GATC 4 cut(s) 12, 19, 66, 228
DpnII GATC 4 cut(s) 10, 17, 64, 226
Eco130I CCWWGG 1 cut(s) 219
Eco88I CYCGRG 1 cut(s) 242
EcoT14I CCWWGG 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 2 cut(s) 120, 208
FaiI YATR 3 cut(s) 90, 118, 206
FalI AAGNNNNNCTT 2 cut(s) 117, 149
FatI CATG 2 cut(s) 116, 204
FbaI TGATCA 2 cut(s) 64, 226
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 2 cut(s) 83, 236
Fsp4HI GCNGC 1 cut(s) 288
GluI GCNGC 1 cut(s) 288
GsuI CTGGAG 2 cut(s) 209, 326
HaeIII GGCC 1 cut(s) 24
Hin1II CATG 2 cut(s) 120, 208
HinfI GANTC 2 cut(s) 79, 313
HphI GGTGA 1 cut(s) 55
Hpy188III TCNNGA 3 cut(s) 68, 244, 317
HpyAV CCTTC 1 cut(s) 324
HpyCH4III ACNGT 1 cut(s) 167
HpyCH4V TGCA 4 cut(s) 101, 163, 236, 290
HpyF10VI GCNNNNNNNGC 1 cut(s) 30
HpyF3I CTNAG 2 cut(s) 129, 356
Hsp92II CATG 2 cut(s) 120, 208
Ksp22I TGATCA 2 cut(s) 64, 226
Kzo9I GATC 4 cut(s) 10, 17, 64, 226
LmnI GCTCC 2 cut(s) 137, 307
LpnPI CCDG 6 cut(s) 6, 53, 167, 173, 290, 302
Lsp1109I GCAGC 1 cut(s) 274
LweI GCATC 3 cut(s) 150, 183, 299
MaeIII GTNAC 2 cut(s) 119, 249
MalI GATC 4 cut(s) 12, 19, 66, 228
MboI GATC 4 cut(s) 10, 17, 64, 226
MflI RGATCY 1 cut(s) 17
MluCI AATT 4 cut(s) 92, 146, 176, 212
MnlI CCTC 3 cut(s) 100, 251, 327
MseI TTAA 1 cut(s) 351
MwoI GCNNNNNNNGC 1 cut(s) 30
NdeII GATC 4 cut(s) 10, 17, 64, 226
NlaIII CATG 2 cut(s) 120, 208
NmuCI GTSAC 1 cut(s) 119
NspI RCATGY 2 cut(s) 120, 208
NspV TTCGAA 1 cut(s) 328
PaeR7I CTCGAG 1 cut(s) 242
PfeI GAWTC 2 cut(s) 79, 313
PflMI CCANNNNNTGG 1 cut(s) 20
PkrI GCNGC 1 cut(s) 289
PsiI TTATAA 1 cut(s) 90
PstNI CAGNNNCTG 1 cut(s) 187
PsuI RGATCY 1 cut(s) 17
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 4 cut(s) 10, 17, 64, 226
SetI ASST 4 cut(s) 45, 130, 135, 357
SfaNI GCATC 3 cut(s) 150, 183, 299
Sfr274I CTCGAG 1 cut(s) 242
SfuI TTCGAA 1 cut(s) 328
SlaI CTCGAG 1 cut(s) 242
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
Sse9I AATT 4 cut(s) 92, 146, 176, 212
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 167
TaqI TCGA 2 cut(s) 243, 328
TasI AATT 4 cut(s) 92, 146, 176, 212
TfiI GAWTC 2 cut(s) 79, 313
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TseFI GTSAC 1 cut(s) 119
TseI GCWGC 1 cut(s) 287
Tsp45I GTSAC 1 cut(s) 119
TspDTI ATGAA 1 cut(s) 85
Van91I CCANNNNNTGG 1 cut(s) 20
XapI RAATTY 3 cut(s) 92, 146, 212
XceI RCATGY 2 cut(s) 120, 208
XhoI CTCGAG 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.