pycom111g00830

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0017134
Physical Location & Seq
Reverse (-)
558110 .. 560349
2240 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g00830.1

Sequence Viewer

Length: 522 bp
ATGCTGTCGAGGGAACAGCTGCTTCATCTTTTCGATCGATTCTCTTTCCTCACATCTCAGCCTGAGGTGAAGAAAAGAATCGCAGATGGTGTTGAGGACAAGCAGGAAGCTGTAGCTGTAACTACTGCGATCCAGGAGGAGATATTTTTGGAGATGGGGATTGATCCAAGATTTGGCCTATCGTGCCTGGGAAAGGTGAATGTTGAATACGAGAATGATCAGGATGTGATGATTCAGTTCTATAAATTCATTGCAAGGGAGGAGATGGCCTGCGATGAAGCTGAGCTTGGAGCAGATGAATTTGCTGAAAGGATGCATAGTCAAGAACAATTACAGGAACAGCAACTGGAGATGCTAAAGCACATGCGTAAATTTCCCATGGATGATCGATCTGCAATCCTCAAGAAGTTGCATCAGCAGATGGAAAACGCCAACTTTGATGGTGCCGCATCTGTTTTGTCACCGGAGCAGATTCAGGAGATTGTTCGAAGGAGGGTGTCTCCTTTATTTAACCCGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

174

Amino Acids

20.15

Weight (kDa)

4.87

Isoelectric Point (pI)

61.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017134)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27050
fragaria_vesca FvH4_6g53561
malus_domestica MD09G1010400.v1.1 MD16G1254400.v1.1
prunus_persica Prupe.3G306700_v2.0.a1
pyrus_communis pycom111g00830
rosa_chinensis RchiOBHm_Chr2g0175601
rosa_laevigata RLG00000022343
rosa_multiflora Rmu_sc0000998.1_g000022 Rmu_sc0012222.1_g000001
rosa_rugosa Rorug02G0586600
rosa_samantha Rh2CG639800
rosa_wichuraiana Rw2G054590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 443
AccB7I CCANNNNNTGG 1 cut(s) 173
AciI CCGC 1 cut(s) 447
AclWI GGATC 2 cut(s) 124, 158
AcsI RAATTY 3 cut(s) 245, 299, 371
AfiI CCNNNNNNNGG 2 cut(s) 173, 193
AgsI TTSAA 1 cut(s) 206
AjnI CCWGG 2 cut(s) 132, 186
AjuI GAANNNNNNNTTGG 2 cut(s) 270, 302
AluBI AGCT 5 cut(s) 19, 110, 116, 281, 286
AluI AGCT 5 cut(s) 19, 110, 116, 281, 286
Alw26I GTCTC 1 cut(s) 504
AlwI GGATC 2 cut(s) 124, 158
AlwNI CAGNNNCTG 1 cut(s) 346
Ama87I CYCGRG 1 cut(s) 514
AoxI GGCC 2 cut(s) 175, 267
ApeKI GCWGC 1 cut(s) 19
ApoI RAATTY 3 cut(s) 245, 299, 371
AsuHPI GGTGA 3 cut(s) 79, 208, 453
AsuII TTCGAA 1 cut(s) 487
AvaI CYCGRG 1 cut(s) 514
AxyI CCTNAGG 1 cut(s) 63
BanI GGYRCC 1 cut(s) 443
BbvI GCAGC 1 cut(s) 6
BccI CCATC 5 cut(s) 80, 148, 259, 415, 434
BciT130I CCWGG 2 cut(s) 134, 188
BclI TGATCA 1 cut(s) 217
BcoDI GTCTC 1 cut(s) 504
BfmI CTRYAG 1 cut(s) 111
BisI GCNGC 2 cut(s) 20, 447
BlpI GCTNAGC 1 cut(s) 282
BlsI GCNGC 2 cut(s) 21, 448
Bme1390I CCNGG 2 cut(s) 134, 188
BmeT110I CYCGRG 1 cut(s) 514
BmiI GGNNCC 1 cut(s) 445
BmrFI CCNGG 2 cut(s) 134, 188
BmsI GCATC 4 cut(s) 303, 342, 421, 458
BplI GAGNNNNNCTC 2 cut(s) 484, 516
BpmI CTGGAG 1 cut(s) 368
Bpu1102I GCTNAGC 1 cut(s) 282
Bpu14I TTCGAA 1 cut(s) 487
BpuEI CTTGAG 1 cut(s) 386
Bsa29I ATCGAT 2 cut(s) 37, 388
BsaJI CCNNGG 2 cut(s) 187, 378
BsaWI WCCGGW 1 cut(s) 463
Bsc4I CCNNNNNNNGG 2 cut(s) 173, 193
Bse1I ACTGG 1 cut(s) 351
Bse21I CCTNAGG 1 cut(s) 63
Bse3DI GCAATG 1 cut(s) 249
BseBI CCWGG 2 cut(s) 134, 188
BseCI ATCGAT 2 cut(s) 37, 388
BseDI CCNNGG 2 cut(s) 187, 378
BseGI GGATG 3 cut(s) 229, 318, 388
BseLI CCNNNNNNNGG 2 cut(s) 173, 193
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 3 cut(s) 54, 71, 273
BseNI ACTGG 1 cut(s) 351
BseRI GAGGAG 2 cut(s) 152, 275
BseXI GCAGC 1 cut(s) 6
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 2 cut(s) 177, 269
BshNI GGYRCC 1 cut(s) 443
BshVI ATCGAT 2 cut(s) 37, 388
BsiEI CGRYCG 1 cut(s) 37
BsiHKCI CYCGRG 1 cut(s) 514
BsiSI CCGG 1 cut(s) 464
BslI CCNNNNNNNGG 2 cut(s) 173, 193
BsmAI GTCTC 1 cut(s) 504
BsnI GGCC 2 cut(s) 177, 269
BsoBI CYCGRG 1 cut(s) 514
Bsp119I TTCGAA 1 cut(s) 487
Bsp143I GATC 6 cut(s) 34, 129, 163, 217, 385, 389
Bsp1720I GCTNAGC 1 cut(s) 282
Bsp19I CCATGG 1 cut(s) 378
BspACI CCGC 1 cut(s) 447
BspANI GGCC 2 cut(s) 177, 269
BspCNI CTCAG 3 cut(s) 55, 70, 274
BspDI ATCGAT 2 cut(s) 37, 388
BspLI GGNNCC 1 cut(s) 445
BspPI GGATC 2 cut(s) 124, 158
BspT104I TTCGAA 1 cut(s) 487
BspT107I GGYRCC 1 cut(s) 443
BsrDI GCAATG 1 cut(s) 249
BsrI ACTGG 1 cut(s) 351
BssECI CCNNGG 2 cut(s) 187, 378
BssMI GATC 6 cut(s) 34, 129, 163, 217, 385, 389
BssT1I CCWWGG 1 cut(s) 378
Bst2UI CCWGG 2 cut(s) 134, 188
BstBI TTCGAA 1 cut(s) 487
BstC8I GCNNGC 1 cut(s) 271
BstDEI CTNAG 3 cut(s) 57, 63, 282
BstDSI CCRYGG 1 cut(s) 378
BstENI CCTNNNNNAGG 1 cut(s) 191
BstF5I GGATG 3 cut(s) 229, 318, 388
BstKTI GATC 6 cut(s) 37, 132, 166, 220, 388, 392
BstMAI GTCTC 1 cut(s) 504
BstMBI GATC 6 cut(s) 34, 129, 163, 217, 385, 389
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 1 cut(s) 183
BstNI CCWGG 2 cut(s) 134, 188
BstNSI RCATGY 1 cut(s) 367
BstSCI CCNGG 2 cut(s) 132, 186
BstSFI CTRYAG 1 cut(s) 111
BstV1I GCAGC 1 cut(s) 6
Bsu15I ATCGAT 2 cut(s) 37, 388
Bsu36I CCTNAGG 1 cut(s) 63
BsuRI GGCC 2 cut(s) 177, 269
BsuTUI ATCGAT 2 cut(s) 37, 388
BtgI CCRYGG 1 cut(s) 378
BtgZI GCGATG 1 cut(s) 288
BtsCI GGATG 3 cut(s) 229, 318, 388
Cac8I GCNNGC 1 cut(s) 271
CaiI CAGNNNCTG 1 cut(s) 346
ClaI ATCGAT 2 cut(s) 37, 388
CviAII CATG 2 cut(s) 364, 379
CviJI RGCY 8 cut(s) 19, 61, 110, 116, 177, 269, 281, 286
CviKI_1 RGCY 8 cut(s) 19, 61, 110, 116, 177, 269, 281, 286
DdeI CTNAG 3 cut(s) 57, 63, 282
DpnI GATC 6 cut(s) 36, 131, 165, 219, 387, 391
DpnII GATC 6 cut(s) 34, 129, 163, 217, 385, 389
Eco130I CCWWGG 1 cut(s) 378
Eco81I CCTNAGG 1 cut(s) 63
Eco88I CYCGRG 1 cut(s) 514
EcoNI CCTNNNNNAGG 1 cut(s) 191
EcoRII CCWGG 2 cut(s) 132, 186
EcoT14I CCWWGG 1 cut(s) 378
EcoT22I ATGCAT 1 cut(s) 318
ErhI CCWWGG 1 cut(s) 378
FaeI CATG 2 cut(s) 367, 382
FaiI YATR 4 cut(s) 243, 318, 365, 380
FalI AAGNNNNNCTT 2 cut(s) 270, 302
FatI CATG 2 cut(s) 363, 378
FbaI TGATCA 1 cut(s) 217
Fnu4HI GCNGC 2 cut(s) 20, 447
FokI GGATG 3 cut(s) 236, 325, 395
Fsp4HI GCNGC 2 cut(s) 20, 447
GluI GCNGC 2 cut(s) 20, 447
GsuI CTGGAG 1 cut(s) 368
HaeIII GGCC 2 cut(s) 177, 269
HapII CCGG 1 cut(s) 464
Hin1II CATG 2 cut(s) 367, 382
HinfI GANTC 4 cut(s) 39, 78, 232, 472
HpaII CCGG 1 cut(s) 464
HphI GGTGA 3 cut(s) 79, 208, 453
Hpy188III TCNNGA 4 cut(s) 221, 323, 403, 476
HpyAV CCTTC 1 cut(s) 483
HpyCH4V TGCA 4 cut(s) 254, 316, 395, 412
HpyF10VI GCNNNNNNNGC 1 cut(s) 183
HpyF3I CTNAG 3 cut(s) 57, 63, 282
Hsp92II CATG 2 cut(s) 367, 382
Ksp22I TGATCA 1 cut(s) 217
Kzo9I GATC 6 cut(s) 34, 129, 163, 217, 385, 389
LmnI GCTCC 2 cut(s) 290, 466
Lsp1109I GCAGC 1 cut(s) 6
LweI GCATC 4 cut(s) 303, 342, 421, 458
MaeIII GTNAC 2 cut(s) 118, 459
MalI GATC 6 cut(s) 36, 131, 165, 219, 387, 391
MboI GATC 6 cut(s) 34, 129, 163, 217, 385, 389
MboII GAAGA 1 cut(s) 82
MluCI AATT 4 cut(s) 245, 299, 329, 371
MnlI CCTC 8 cut(s) 3, 58, 59, 88, 130, 253, 410, 486
Mph1103I ATGCAT 1 cut(s) 318
MseI TTAA 1 cut(s) 510
MspA1I CMGCKG 1 cut(s) 19
MspI CCGG 1 cut(s) 464
MspR9I CCNGG 2 cut(s) 134, 188
MvaI CCWGG 2 cut(s) 134, 188
MwoI GCNNNNNNNGC 1 cut(s) 183
NcoI CCATGG 1 cut(s) 378
NdeII GATC 6 cut(s) 34, 129, 163, 217, 385, 389
NlaIII CATG 2 cut(s) 367, 382
NlaIV GGNNCC 1 cut(s) 445
NmuCI GTSAC 1 cut(s) 459
NsiI ATGCAT 1 cut(s) 318
NspI RCATGY 1 cut(s) 367
NspV TTCGAA 1 cut(s) 487
PfeI GAWTC 4 cut(s) 39, 78, 232, 472
PflMI CCANNNNNTGG 1 cut(s) 173
PfoI TCCNGGA 1 cut(s) 132
PkrI GCNGC 2 cut(s) 21, 448
Ple19I CGATCG 1 cut(s) 37
Psp6I CCWGG 2 cut(s) 132, 186
PspGI CCWGG 2 cut(s) 132, 186
PspN4I GGNNCC 1 cut(s) 445
PstNI CAGNNNCTG 1 cut(s) 346
PvuI CGATCG 1 cut(s) 37
PvuII CAGCTG 1 cut(s) 19
SaqAI TTAA 1 cut(s) 510
SatI GCNGC 2 cut(s) 20, 447
Sau3AI GATC 6 cut(s) 34, 129, 163, 217, 385, 389
ScrFI CCNGG 2 cut(s) 134, 188
SetI ASST 7 cut(s) 21, 69, 112, 118, 198, 283, 288
SfaNI GCATC 4 cut(s) 303, 342, 421, 458
SfcI CTRYAG 1 cut(s) 111
SfuI TTCGAA 1 cut(s) 487
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
Sse9I AATT 4 cut(s) 245, 299, 329, 371
SsiI CCGC 1 cut(s) 447
StyD4I CCNGG 2 cut(s) 132, 186
StyI CCWWGG 1 cut(s) 378
TaqI TCGA 5 cut(s) 8, 33, 37, 388, 487
TasI AATT 4 cut(s) 245, 299, 329, 371
TauI GCSGC 1 cut(s) 449
TfiI GAWTC 4 cut(s) 39, 78, 232, 472
Tru1I TTAA 1 cut(s) 510
Tru9I TTAA 1 cut(s) 510
TseFI GTSAC 1 cut(s) 459
TseI GCWGC 1 cut(s) 19
Tsp45I GTSAC 1 cut(s) 459
TspDTI ATGAA 4 cut(s) 14, 238, 291, 312
Van91I CCANNNNNTGG 1 cut(s) 173
XagI CCTNNNNNAGG 1 cut(s) 191
XapI RAATTY 3 cut(s) 245, 299, 371
XceI RCATGY 1 cut(s) 367
Zsp2I ATGCAT 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.