MD16G1267100.v1.1

Calmodulin binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007252
Physical Location & Seq
Forward (+)
34120560 .. 34127581
7022 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1267100.v1.1.491

Sequence Viewer

Length: 1899 bp
ATGCACACTAGACTTATGGAAAGGACCAACTCTATGAGAGGGAAGAGGCCAATGGAAGCGGGAGAGGAGGAGCAGGCTGAGCGAAAGCGTCCTGCTCTCGCTAGTGTAATTATAGAAGCTCTCAAGGTGGACAGTTTGCAGAAGCTATGCTCGTCATTGGAACCCATTCTTCGCAGAGTTGTAAGCGAAGAAGTGGAGCGTGCTTTGGCTAAGTTAGGCCCTGCGAGAGTTAATGGAAGGTCTTCCCCAAAACGAATTGAAGGTCCAAATGGCCAAAACTTGCAACTAGAGTTTAAGTCCAATTTGGCTCTTCCGATATTCACAGGAGGGAAAGTTGAAGGCGAGCAGGGTGCTGCAATTCATGTTGTTTTGGTTGATACAAACACTGGCCGAGTTGTAACATCTGGACCTGAATCCTCTGTAAAACTAGATATTGTTGTGCTTGAAGGTGATTTCAACAATGAAGATGATGAAGGCTGGACTCAAGAAGAATTTGATACCCATGTGGTGAAAGAGCGTGAAGGAAAGAGACCTCTATTGACTGGGGACCTGCAAGTGACCCTCAAAGAAGGGGTGGGAACTCTAGGGGATCTAACCTTCACAGATAACTCAAGTTGGATACGAAGCAGGAAGTTCAGACTTGGATTGAAAGTAGCTTCGGGATTTTGTGAGGGCATGCGCATACGCGAAGCTAAAACAGAAGCTTTTACTGTTAAAGATCACAGAGGGGAATTGTACAAGAAACACTACCCACCGGCATTAAATGATGAAGTATGGAGATTGGAGAAGATTGGCAAGGATGGGGCATTCCATAAGAGGTTAAACAAAGCAGGAATATTCACAGTTGAAGAGTTTCTTCAGCTTGTCGTCCAAGATTCTCAAAAACTACGGAATATCCTTGGAAGTGGAATGTCAAACAAGATGTGGGAGGCTCTTTTAGAACATGCAAAGACGTGTGTCTTGAGTGGGAAGCTTTATGTTTATTATACTGAAGATACAAGAAATGTTGGTGTTGTTTTTAACAACATCTATGAGCTGAGTGGCCTCATCGCTGGGGAGCAGTTTCACTCTGCTGATGCTCTTTCTGACAGCCAGAAGGTCTACGTAGATGCGCTGGTGAAGAAAGCATATGACAACTGGGATCAAGTTATTCAGTATGATGGCAAGTCACTTCTAAACTTCAAGCAGAGTAAGAGGCCAGCTGCACCCCGAACTGAATTTCAAATGGGCCCAATCAATTACCCTGAAGCTTCTGATCAACTGCAACTACATCGAACGTCAAATTCAGTTCACTCAGAGCAGCCTCCATTGGATCCAGCCCTACCAATTGGAGGGTATAATGACAGTCTATCAACAAGATACTTGACCCAGCCACTTGTAAATTCCAATTCTCATACCCAGTTTGATGGCGCCGGATTTGCACTGGATGACCAATTGGTCAGCAATTCTCATCAGGTACAAAGCACAAGAAATGACCACAATGCTGTTGGTTTGGCTCGTGGTCCTCCACAATCATCTACATCAGGATTTCAGACTATCAATCCTGCTCAGACATCCACTCTTAATCCTATAGATGACTGGACAACCAACCGAGATTTCTTTTCAGAGGACGAGATTCGCATTAGGAGTCATGAGATGCTTGAAAATGAGGATATGCAGCACTTGCTTAGAATCTTAAGTATGGGAGGAGGCCATGGCTCCATTGATGTGCCCGATGATGGGTTTTCGATCCCCTCATATATGCCCTCACCGATGCCCAACTTTGAGGAAGATCGCACCCGTCCTGGAAAAGCTGTTGTGGGGTGGCTGAAAATTAAGGCAGCAATGAGATGGGGTTTCTTTGTGAGGAAGAAAGCTGCTGAGAGACGGGCACAGATTGTGGAGATAGAGGATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

633

Amino Acids

70.73

Weight (kDa)

5.9

Isoelectric Point (pI)

46.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 94 - 241 8.6e-65 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 253 - 319 5.5e-33 Calmodulin binding protein central domain
Calmod_bind_C PF20452 324 - 386 2.8e-24 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1436
Acc16I TGCGCA 1 cut(s) 680
Acc36I ACCTGC 1 cut(s) 558
AccB1I GGYRCC 1 cut(s) 1409
AccI GTMKAC 1 cut(s) 1101
AccII CGCG 1 cut(s) 687
AciI CCGC 1 cut(s) 59
AclWI GGATC 5 cut(s) 597, 1149, 1307, 1320, 1723
AcoI YGGCCR 2 cut(s) 271, 388
AcsI RAATTY 4 cut(s) 491, 1217, 1282, 1381
AcuI CTGAAG 3 cut(s) 842, 1011, 1266
AcyI GRCGYC 1 cut(s) 1410
AfaI GTAC 2 cut(s) 737, 1458
AfiI CCNNNNNNNGG 3 cut(s) 1331, 1718, 1719
AflII CTTAAG 1 cut(s) 1675
AflIII ACRYGT 1 cut(s) 953
AgsI TTSAA 9 cut(s) 260, 338, 446, 457, 649, 848, 1183, 1223, 1643
AjiI CACGTC 1 cut(s) 954
AjnI CCWGG 1 cut(s) 1783
AjuI GAANNNNNNNTTGG 2 cut(s) 1580, 1612
Alw26I GTCTC 2 cut(s) 523, 1858
AlwI GGATC 5 cut(s) 597, 1149, 1307, 1320, 1723
AoxI GGCC 8 cut(s) 47, 217, 271, 388, 1042, 1196, 1228, 1690
ApaI GGGCCC 1 cut(s) 1232
ApeKI GCWGC 6 cut(s) 353, 1202, 1300, 1657, 1820, 1856
ApoI RAATTY 4 cut(s) 491, 1217, 1282, 1381
ArsI GACNNNNNNTTYG 2 cut(s) 247, 279
Asp700I GAANNNNTTC 3 cut(s) 165, 241, 852
AspLEI GCGC 3 cut(s) 681, 1114, 1412
AspS9I GGNCC 8 cut(s) 24, 218, 263, 407, 547, 1228, 1229, 1502
AsuHPI GGTGA 4 cut(s) 461, 520, 1129, 1740
AvaII GGWCC 5 cut(s) 24, 263, 407, 547, 1502
BaeGI GKGCMC 3 cut(s) 1232, 1713, 1873
BaeI ACNNNNGTAYC 2 cut(s) 489, 522
BalI TGGCCA 1 cut(s) 273
BamHI GGATCC 1 cut(s) 1312
BanI GGYRCC 1 cut(s) 1409
BanII GRGCYC 1 cut(s) 1232
BauI CACGAG 1 cut(s) 1497
BbsI GAAGAC 1 cut(s) 234
BbvI GCAGC 6 cut(s) 340, 1189, 1312, 1669, 1832, 1843
BccI CCATC 5 cut(s) 794, 1154, 1400, 1712, 1824
BcgI CGANNNNNNTGC 4 cut(s) 332, 366, 1253, 1287
BciT130I CCWGG 1 cut(s) 1785
BciVI GTATCC 1 cut(s) 612
BclI TGATCA 1 cut(s) 1255
BcoDI GTCTC 2 cut(s) 523, 1858
BfaI CTAG 5 cut(s) 9, 102, 287, 428, 584
BfmI CTRYAG 1 cut(s) 1569
BfoI RGCGCY 1 cut(s) 1413
BfrI CTTAAG 1 cut(s) 1675
BfuAI ACCTGC 1 cut(s) 558
BfuI GTATCC 1 cut(s) 612
BisI GCNGC 6 cut(s) 354, 1203, 1301, 1658, 1821, 1857
BlpI GCTNAGC 1 cut(s) 78
BlsI GCNGC 6 cut(s) 355, 1204, 1302, 1659, 1822, 1858
Bme1390I CCNGG 1 cut(s) 1785
Bme18I GGWCC 5 cut(s) 24, 263, 407, 547, 1502
BmgBI CACGTC 1 cut(s) 954
BmgT120I GGNCC 8 cut(s) 24, 218, 263, 407, 547, 1228, 1229, 1502
BmiI GGNNCC 6 cut(s) 162, 548, 1230, 1314, 1411, 1699
BmrFI CCNGG 1 cut(s) 1785
BmrI ACTGGG 3 cut(s) 552, 1147, 1393
BmsI GCATC 4 cut(s) 1066, 1099, 1626, 1743
BmuI ACTGGG 3 cut(s) 552, 1147, 1393
BoxI GACNNNNGTC 1 cut(s) 956
BpiI GAAGAC 1 cut(s) 234
Bpu1102I GCTNAGC 1 cut(s) 78
BpuEI CTTGAG 4 cut(s) 107, 468, 595, 982
BsaAI YACGTR 1 cut(s) 1105
BsaHI GRCGYC 1 cut(s) 1410
BsaI GGTCTC 1 cut(s) 523
BsaJI CCNNGG 2 cut(s) 898, 1693
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bsc4I CCNNNNNNNGG 3 cut(s) 1331, 1718, 1719
Bse118I RCCGGY 1 cut(s) 754
Bse1I ACTGG 6 cut(s) 391, 547, 1142, 1399, 1428, 1583
Bse3DI GCAATG 1 cut(s) 1830
BseBI CCWGG 1 cut(s) 1785
BseDI CCNNGG 2 cut(s) 898, 1693
BseGI GGATG 4 cut(s) 805, 1432, 1553, 1897
BseLI CCNNNNNNNGG 3 cut(s) 1331, 1718, 1719
BseMI GCAATG 1 cut(s) 1830
BseMII CTCAG 5 cut(s) 69, 1028, 1308, 1562, 1851
BseNI ACTGG 6 cut(s) 391, 547, 1142, 1399, 1428, 1583
BseRI GAGGAG 3 cut(s) 80, 83, 1701
BseSI GKGCMC 3 cut(s) 1232, 1713, 1873
BseXI GCAGC 6 cut(s) 340, 1189, 1312, 1669, 1832, 1843
BseYI CCCAGC 2 cut(s) 1052, 1368
BsgI GTGCAG 1 cut(s) 1188
Bsh1236I CGCG 1 cut(s) 687
BshFI GGCC 8 cut(s) 49, 219, 273, 390, 1044, 1198, 1230, 1692
BshNI GGYRCC 1 cut(s) 1409
BsiSI CCGG 2 cut(s) 755, 1413
BslFI GGGAC 1 cut(s) 560
BslI CCNNNNNNNGG 3 cut(s) 1331, 1718, 1719
BsmAI GTCTC 2 cut(s) 523, 1858
BsmBI CGTCTC 1 cut(s) 1858
BsmFI GGGAC 1 cut(s) 560
BsmI GAATGC 1 cut(s) 806
BsnI GGCC 8 cut(s) 49, 219, 273, 390, 1044, 1198, 1230, 1692
Bso31I GGTCTC 1 cut(s) 523
Bsp120I GGGCCC 1 cut(s) 1228
Bsp1286I GDGCHC 3 cut(s) 1232, 1713, 1873
Bsp1407I TGTACA 1 cut(s) 735
Bsp143I GATC 7 cut(s) 589, 718, 1141, 1255, 1312, 1728, 1771
Bsp1720I GCTNAGC 1 cut(s) 78
Bsp19I CCATGG 1 cut(s) 1693
BspACI CCGC 1 cut(s) 59
BspANI GGCC 8 cut(s) 49, 219, 273, 390, 1044, 1198, 1230, 1692
BspCNI CTCAG 5 cut(s) 70, 1029, 1307, 1561, 1852
BspFNI CGCG 1 cut(s) 687
BspHI TCATGA 1 cut(s) 1630
BspLI GGNNCC 6 cut(s) 162, 548, 1230, 1314, 1411, 1699
BspMI ACCTGC 1 cut(s) 558
BspPI GGATC 5 cut(s) 597, 1149, 1307, 1320, 1723
BspQI GCTCTTC 1 cut(s) 315
BspT107I GGYRCC 1 cut(s) 1409
BspTI CTTAAG 1 cut(s) 1675
BspTNI GGTCTC 1 cut(s) 523
BsrDI GCAATG 1 cut(s) 1830
BsrFI RCCGGY 1 cut(s) 754
BsrGI TGTACA 1 cut(s) 735
BsrI ACTGG 6 cut(s) 391, 547, 1142, 1399, 1428, 1583
BssAI RCCGGY 1 cut(s) 754
BssECI CCNNGG 2 cut(s) 898, 1693
BssMI GATC 7 cut(s) 589, 718, 1141, 1255, 1312, 1728, 1771
BssNI GRCGYC 1 cut(s) 1410
BssSI CACGAG 1 cut(s) 1497
BssT1I CCWWGG 2 cut(s) 898, 1693
Bst2BI CACGAG 1 cut(s) 1497
Bst2UI CCWGG 1 cut(s) 1785
Bst4CI ACNGT 4 cut(s) 134, 712, 844, 1346
Bst6I CTCTTC 3 cut(s) 38, 315, 843
BstACI GRCGYC 1 cut(s) 1410
BstAFI CTTAAG 1 cut(s) 1675
BstAPI GCANNNNNTGC 1 cut(s) 1663
BstAUI TGTACA 1 cut(s) 735
BstBAI YACGTR 1 cut(s) 1105
BstC8I GCNNGC 5 cut(s) 75, 201, 344, 677, 1200
BstDEI CTNAG 7 cut(s) 78, 210, 1037, 1294, 1548, 1667, 1860
BstDSI CCRYGG 1 cut(s) 1693
BstF5I GGATG 4 cut(s) 805, 1432, 1553, 1897
BstFNI CGCG 1 cut(s) 687
BstH2I RGCGCY 1 cut(s) 1413
BstHHI GCGC 3 cut(s) 681, 1114, 1412
BstKTI GATC 7 cut(s) 592, 721, 1144, 1258, 1315, 1731, 1774
BstMAI GTCTC 2 cut(s) 523, 1858
BstMBI GATC 7 cut(s) 589, 718, 1141, 1255, 1312, 1728, 1771
BstMWI GCNNNNNNNGC 3 cut(s) 79, 1418, 1663
BstNI CCWGG 1 cut(s) 1785
BstNSI RCATGY 2 cut(s) 679, 947
BstPAI GACNNNNGTC 1 cut(s) 956
BstSCI CCNGG 1 cut(s) 1783
BstSFI CTRYAG 1 cut(s) 1569
BstSLI GKGCMC 3 cut(s) 1232, 1713, 1873
BstSNI TACGTA 1 cut(s) 1105
BstUI CGCG 1 cut(s) 687
BstV1I GCAGC 6 cut(s) 340, 1189, 1312, 1669, 1832, 1843
BstV2I GAAGAC 1 cut(s) 234
BstX2I RGATCY 2 cut(s) 589, 1312
BstXI CCANNNNNNTGG 1 cut(s) 1406
BstYI RGATCY 2 cut(s) 589, 1312
BsuI GTATCC 1 cut(s) 612
BsuRI GGCC 8 cut(s) 49, 219, 273, 390, 1044, 1198, 1230, 1692
BtgI CCRYGG 1 cut(s) 1693
BtgZI GCGATG 1 cut(s) 1033
BtrI CACGTC 1 cut(s) 954
BtsCI GGATG 4 cut(s) 805, 1432, 1553, 1897
BtsIMutI CAGTG 2 cut(s) 384, 1421
BveI ACCTGC 1 cut(s) 558
Cac8I GCNNGC 5 cut(s) 75, 201, 344, 677, 1200
CciI TCATGA 1 cut(s) 1630
CfoI GCGC 3 cut(s) 681, 1114, 1412
Cfr10I RCCGGY 1 cut(s) 754
Cfr13I GGNCC 8 cut(s) 24, 218, 263, 407, 547, 1228, 1229, 1502
CseI GACGC 1 cut(s) 77
Csp6I GTAC 2 cut(s) 736, 1457
CviAII CATG 6 cut(s) 362, 503, 676, 944, 1631, 1694
CviQI GTAC 2 cut(s) 736, 1457
DdeI CTNAG 7 cut(s) 78, 210, 1037, 1294, 1548, 1667, 1860
DinI GGCGCC 1 cut(s) 1411
DpnI GATC 7 cut(s) 591, 720, 1143, 1257, 1314, 1730, 1773
DpnII GATC 7 cut(s) 589, 718, 1141, 1255, 1312, 1728, 1771
DrdI GACNNNNNNGTC 1 cut(s) 1436
DseDI GACNNNNNNGTC 1 cut(s) 1436
EaeI YGGCCR 2 cut(s) 271, 388
Eam1104I CTCTTC 3 cut(s) 38, 315, 843
EarI CTCTTC 3 cut(s) 38, 315, 843
Eco105I TACGTA 1 cut(s) 1105
Eco130I CCWWGG 2 cut(s) 898, 1693
Eco24I GRGCYC 1 cut(s) 1232
Eco31I GGTCTC 1 cut(s) 523
Eco47I GGWCC 5 cut(s) 24, 263, 407, 547, 1502
Eco57I CTGAAG 3 cut(s) 842, 1011, 1266
EcoO109I RGGNCCY 2 cut(s) 218, 547
EcoRII CCWGG 1 cut(s) 1783
EcoT14I CCWWGG 2 cut(s) 898, 1693
EcoT38I GRGCYC 1 cut(s) 1232
EgeI GGCGCC 1 cut(s) 1411
EheI GGCGCC 1 cut(s) 1411
ErhI CCWWGG 2 cut(s) 898, 1693
Esp3I CGTCTC 1 cut(s) 1858
FaeI CATG 6 cut(s) 365, 506, 679, 947, 1634, 1697
FalI AAGNNNNNCTT 2 cut(s) 840, 872
FaqI GGGAC 1 cut(s) 560
FatI CATG 6 cut(s) 361, 502, 675, 943, 1630, 1693
FauI CCCGC 1 cut(s) 52
FauNDI CATATG 1 cut(s) 1129
FbaI TGATCA 1 cut(s) 1255
FblI GTMKAC 1 cut(s) 1101
Fnu4HI GCNGC 6 cut(s) 354, 1203, 1301, 1658, 1821, 1857
FokI GGATG 3 cut(s) 812, 1439, 1540
FriOI GRGCYC 1 cut(s) 1232
Fsp4HI GCNGC 6 cut(s) 354, 1203, 1301, 1658, 1821, 1857
FspAI RTGCGCAY 1 cut(s) 680
FspBI CTAG 5 cut(s) 9, 102, 287, 428, 584
FspI TGCGCA 1 cut(s) 680
GlaI GCGC 3 cut(s) 680, 1113, 1411
GluI GCNGC 6 cut(s) 354, 1203, 1301, 1658, 1821, 1857
GsaI CCCAGC 2 cut(s) 1056, 1372
HaeII RGCGCY 1 cut(s) 1413
HaeIII GGCC 8 cut(s) 49, 219, 273, 390, 1044, 1198, 1230, 1692
HapII CCGG 2 cut(s) 755, 1413
HgaI GACGC 1 cut(s) 77
HhaI GCGC 3 cut(s) 681, 1114, 1412
Hin1I GRCGYC 1 cut(s) 1410
Hin1II CATG 6 cut(s) 365, 506, 679, 947, 1634, 1697
Hin6I GCGC 3 cut(s) 679, 1112, 1410
HinP1I GCGC 3 cut(s) 679, 1112, 1410
HindIII AAGCTT 3 cut(s) 702, 971, 1248
HinfI GANTC 6 cut(s) 413, 481, 875, 1615, 1627, 1671
HpaII CCGG 2 cut(s) 755, 1413
HphI GGTGA 4 cut(s) 461, 520, 1129, 1740
Hpy166II GTNNAC 3 cut(s) 130, 1102, 1291
Hpy188I TCNGA 8 cut(s) 315, 638, 1087, 1255, 1297, 1533, 1551, 1606
Hpy188III TCNNGA 6 cut(s) 405, 485, 660, 961, 1524, 1631
Hpy8I GTNNAC 3 cut(s) 130, 1102, 1291
HpyAV CCTTC 9 cut(s) 231, 254, 332, 440, 467, 515, 563, 607, 1090
HpyCH4III ACNGT 4 cut(s) 134, 712, 844, 1346
HpyCH4IV ACGT 3 cut(s) 953, 1104, 1277
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 1418, 1663
HpyF3I CTNAG 7 cut(s) 78, 210, 1037, 1294, 1548, 1667, 1860
HpySE526I ACGT 3 cut(s) 953, 1104, 1277
Hsp92I GRCGYC 1 cut(s) 1410
Hsp92II CATG 6 cut(s) 365, 506, 679, 947, 1634, 1697
HspAI GCGC 3 cut(s) 679, 1112, 1410
KasI GGCGCC 1 cut(s) 1409
Ksp22I TGATCA 1 cut(s) 1255
Kzo9I GATC 7 cut(s) 589, 718, 1141, 1255, 1312, 1728, 1771
LguI GCTCTTC 1 cut(s) 315
LmnI GCTCC 4 cut(s) 70, 196, 1057, 1703
Lsp1109I GCAGC 6 cut(s) 340, 1189, 1312, 1669, 1832, 1843
LweI GCATC 4 cut(s) 1066, 1099, 1626, 1743
MaeI CTAG 5 cut(s) 9, 102, 287, 428, 584
MaeII ACGT 3 cut(s) 953, 1104, 1277
MaeIII GTNAC 3 cut(s) 397, 556, 1167
MalI GATC 7 cut(s) 591, 720, 1143, 1257, 1314, 1730, 1773
MboI GATC 7 cut(s) 589, 718, 1141, 1255, 1312, 1728, 1771
MfeI CAATTG 2 cut(s) 1326, 1433
MflI RGATCY 2 cut(s) 589, 1312
MhlI GDGCHC 3 cut(s) 1232, 1713, 1873
MlsI TGGCCA 1 cut(s) 273
MluNI TGGCCA 1 cut(s) 273
Mly113I GGCGCC 1 cut(s) 1410
MlyI GAGTC 2 cut(s) 475, 1636
MmeI TCCRAC 1 cut(s) 596
Mox20I TGGCCA 1 cut(s) 273
MroXI GAANNNNTTC 3 cut(s) 165, 241, 852
MscI TGGCCA 1 cut(s) 273
MseI TTAA 9 cut(s) 231, 294, 714, 761, 821, 1020, 1563, 1676, 1815
MslI CAYNNNNRTG 1 cut(s) 1706
Msp20I TGGCCA 1 cut(s) 273
MspA1I CMGCKG 1 cut(s) 1202
MspCI CTTAAG 1 cut(s) 1675
MspI CCGG 2 cut(s) 755, 1413
MspR9I CCNGG 1 cut(s) 1785
MunI CAATTG 2 cut(s) 1326, 1433
Mva1269I GAATGC 1 cut(s) 806
MvaI CCWGG 1 cut(s) 1785
MvnI CGCG 1 cut(s) 687
MwoI GCNNNNNNNGC 3 cut(s) 79, 1418, 1663
NarI GGCGCC 1 cut(s) 1410
NcoI CCATGG 1 cut(s) 1693
NdeI CATATG 1 cut(s) 1129
NdeII GATC 7 cut(s) 589, 718, 1141, 1255, 1312, 1728, 1771
NlaIII CATG 6 cut(s) 365, 506, 679, 947, 1634, 1697
NlaIV GGNNCC 6 cut(s) 162, 548, 1230, 1314, 1411, 1699
NmeAIII GCCGAG 1 cut(s) 416
NmuCI GTSAC 2 cut(s) 556, 1167
NsbI TGCGCA 1 cut(s) 680
NspI RCATGY 2 cut(s) 679, 947
PaeI GCATGC 1 cut(s) 679
PagI TCATGA 1 cut(s) 1630
PciSI GCTCTTC 1 cut(s) 315
PctI GAATGC 1 cut(s) 806
PdmI GAANNNNTTC 3 cut(s) 165, 241, 852
PfeI GAWTC 4 cut(s) 413, 875, 1615, 1671
PfoI TCCNGGA 1 cut(s) 1783
PkrI GCNGC 6 cut(s) 355, 1204, 1302, 1659, 1822, 1858
PleI GAGTC 2 cut(s) 475, 1635
PluTI GGCGCC 1 cut(s) 1413
PpsI GAGTC 2 cut(s) 475, 1635
Ppu21I YACGTR 1 cut(s) 1105
PpuMI RGGWCCY 1 cut(s) 547
PshAI GACNNNNGTC 1 cut(s) 956
Psp5II RGGWCCY 1 cut(s) 547
Psp6I CCWGG 1 cut(s) 1783
PspFI CCCAGC 2 cut(s) 1052, 1368
PspGI CCWGG 1 cut(s) 1783
PspN4I GGNNCC 6 cut(s) 162, 548, 1230, 1314, 1411, 1699
PspOMI GGGCCC 1 cut(s) 1228
PspPI GGNCC 8 cut(s) 24, 218, 263, 407, 547, 1228, 1229, 1502
PspPPI RGGWCCY 1 cut(s) 547
PsuI RGATCY 2 cut(s) 589, 1312
PvuII CAGCTG 1 cut(s) 1202
RsaI GTAC 2 cut(s) 737, 1458
RsaNI GTAC 2 cut(s) 736, 1457
RseI CAYNNNNRTG 1 cut(s) 1706
SapI GCTCTTC 1 cut(s) 315
SaqAI TTAA 9 cut(s) 231, 294, 714, 761, 821, 1020, 1563, 1676, 1815
SatI GCNGC 6 cut(s) 354, 1203, 1301, 1658, 1821, 1857
Sau3AI GATC 7 cut(s) 589, 718, 1141, 1255, 1312, 1728, 1771
Sau96I GGNCC 8 cut(s) 24, 218, 263, 407, 547, 1228, 1229, 1502
SchI GAGTC 2 cut(s) 475, 1636
ScrFI CCNGG 1 cut(s) 1785
SduI GDGCHC 3 cut(s) 1232, 1713, 1873
SfaNI GCATC 4 cut(s) 1066, 1099, 1626, 1743
SfcI CTRYAG 1 cut(s) 1569
SfoI GGCGCC 1 cut(s) 1411
SinI GGWCC 5 cut(s) 24, 263, 407, 547, 1502
SmiMI CAYNNNNRTG 1 cut(s) 1706
SmlI CTYRAG 5 cut(s) 122, 483, 610, 961, 1675
SmoI CTYRAG 5 cut(s) 122, 483, 610, 961, 1675
SnaBI TACGTA 1 cut(s) 1105
SphI GCATGC 1 cut(s) 679
SsiI CCGC 1 cut(s) 59
SspDI GGCGCC 1 cut(s) 1409
SspI AATATT 1 cut(s) 837
SspMI CTAG 5 cut(s) 9, 102, 287, 428, 584
StyD4I CCNGG 1 cut(s) 1783
StyI CCWWGG 2 cut(s) 898, 1693
TaaI ACNGT 4 cut(s) 134, 712, 844, 1346
TaiI ACGT 3 cut(s) 956, 1107, 1280
TaqI TCGA 2 cut(s) 1273, 1727
TatI WGTACW 1 cut(s) 735
TfiI GAWTC 4 cut(s) 413, 875, 1615, 1671
Tru1I TTAA 9 cut(s) 231, 294, 714, 761, 821, 1020, 1563, 1676, 1815
Tru9I TTAA 9 cut(s) 231, 294, 714, 761, 821, 1020, 1563, 1676, 1815
TscAI CASTG 2 cut(s) 391, 1428
TseFI GTSAC 2 cut(s) 556, 1167
TseI GCWGC 6 cut(s) 353, 1202, 1300, 1657, 1820, 1856
Tsp45I GTSAC 2 cut(s) 556, 1167
TspDTI ATGAA 4 cut(s) 350, 477, 486, 783
TspGWI ACGGA 1 cut(s) 904
TspRI CASTG 2 cut(s) 391, 1428
Vha464I CTTAAG 1 cut(s) 1675
VpaK11BI GGWCC 5 cut(s) 24, 263, 407, 547, 1502
XapI RAATTY 4 cut(s) 491, 1217, 1282, 1381
XceI RCATGY 2 cut(s) 679, 947
XcmI CCANNNNNNNNNTGG 1 cut(s) 1484
XmiI GTMKAC 1 cut(s) 1101
XmnI GAANNNNTTC 3 cut(s) 165, 241, 852
XspI CTAG 5 cut(s) 9, 102, 287, 428, 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.