Rh4DG109500

Calmodulin binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0007252
Physical Location & Seq
Reverse (-)
16983074 .. 16996351
13278 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG109500.1

Sequence Viewer

Length: 1746 bp
ATGGTCAGTGAAGAAGTGGAACGTGCATTGGCCAAGTTGGGCCCTCCAAGACTAAATGGAAGGTCTTCCCCCAAACGGATTGAAGGTTCAAATGATCGGAACTTGCAACTGCAGTTTAAGTCCAATCTGGCTCTCCCCATTTTCACAGGAGGGAAAGTTGAAGGCGAGCAGGGGGCTTCAATTCATGTTGTTTTAGTTGATATGAACACTCAGCGTGTTGTAACATCTGGACCTGAATCCTCTATAAAACTAGATATTGTTGTGCTTGAGGGTGATTTTAACAATGAAGATGATGAAGGCTGGACTGAAGAAGAATTTGAAAGCCATGTGGTCAAAGAACGTGAAGGAAAGAGACCTCTTTTGACCGGGGAATTGCAAGTGACACTGAAGGAAGGGGTAGGGACAATTGGGGATCTGACTTTTACAGATAACTCAAGTTGGATACGCAGCAGGAAGTTTAGGCTTGGATTGAAAGTTGCTTCAGGATTTTGTGAGGGCATGCGCATACGTGAAGCGAAAACAGATGCTTTTACTGTTAAAGATCACAGAGGGGAATTGTACAAGAAACATTACCCACCAGCACTAAATGATGACGTATGGAGACTGGAGAAGATCGGAAAGGATGGGGCATTCCATAAGAGGCTAAACAATTCAGGAATACACACAGTTGAACAGTTTCTTCAGCTTGTGGTCAAAGATCCTCAAAAATTACGGAGTATCCTTGGAAGTGGCATGTCAAATAAGATGTGGGAAGCTCTCCTAGAGCATGCAAAGACTTGTGCCCTTAGCGGAAAGCTTTATGTTTATTATCCTGAAGATACAAGAAATGTCGGTGTTGCTTTTAACAACATCTATGAGCTGAGTGGCTTGATTGCTGGGGAACAGTATCATTCTGCCGATACTCTTTCCGACAGCCAGAAGCTCTATGTAGATACGCTTGTGAAGAAAGCATATGAAAATTGGGATCAGGTTGTTGAGTATGATGGTAAATCACTTCTGAACACCAAACAAAATAAGAGGTCCACTGCAGCCCGAAACGAATTTCAAATGGGCTCAATCAGTTACTCCGGTTCTTCAGACCAGCATCCGCAACTACCTCGCCTGACAAATCCAGTTCCCTCAGAACAGACTCTTCTATCTGATTCAGCCCTACCGATTGGAGGGTATAATGATAATCTGTCAACAAGGTATATGACCCAGCAACAAATTGTGAATTCAAATTCCCGTGCCCAGTTTGAAGGCACCTCATTTGCACTAGATGGCCAGTTGATCAACAGCTCTCATCAGCCTCAAAGCATAACAAATGATGCCAATGCTGTTGGCCTGGCCCTTGGTCCTCCACAATCATCGACATCAGGATTTCAGGCTATAAATTCTTCTATTCAGCCGTCTACTCTTAATCCTTTAGATGACTGGGGTACAAGCCGAGAAAAAGGAGTTGATGACTTCTTCTCAGAAGAAGATATTCGCATTAGAAGTCATGAGATGCTTGAAAATGAGGATATGCAGCACTTGCTGAGAATCTTCAGTATGGGAGGCCATGCCTCTATTGATATGCCTGATGATGGATATGCATACCCATATATGCCATCTCCAATGCCCAACTTTGATGAAGATCGCACACGTCCTGGCAAAGCTGTTGTTGGGTGGCTGAAAATTAAGGCAGCTATGAGATGGGGTTTCTTTGTCAGAAAGAAAGCAGCTGAAAGGCGGCGGGCACAGCTTGTGGAGTTAGATGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

581

Amino Acids

65.0

Weight (kDa)

5.6

Isoelectric Point (pI)

44.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 35 - 182 6.7e-65 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 194 - 260 6.9e-34 Calmodulin binding protein central domain
Calmod_bind_C PF20452 265 - 327 2.4e-25 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 503
AccB1I GGYRCC 1 cut(s) 1241
AccI GTMKAC 1 cut(s) 1391
AciI CCGC 4 cut(s) 789, 1088, 1711, 1714
AclWI GGATC 3 cut(s) 420, 692, 972
AcoI YGGCCR 2 cut(s) 30, 1261
AcsI RAATTY 5 cut(s) 314, 1040, 1213, 1219, 1372
AcuI CTGAAG 7 cut(s) 327, 407, 465, 665, 834, 1059, 1510
AfaI GTAC 2 cut(s) 560, 1420
AfiI CCNNNNNNNGG 3 cut(s) 75, 1160, 1565
AflIII ACRYGT 1 cut(s) 1622
AjiI CACGTC 1 cut(s) 1625
AjnI CCWGG 2 cut(s) 1323, 1627
AloI GAACNNNNNNTCC 2 cut(s) 70, 102
Alw26I GTCTC 2 cut(s) 346, 595
AlwI GGATC 3 cut(s) 420, 692, 972
AoxI GGCC 6 cut(s) 30, 40, 1261, 1321, 1326, 1537
ApaI GGGCCC 1 cut(s) 44
ApeKI GCWGC 5 cut(s) 447, 1028, 1507, 1664, 1700
ApoI RAATTY 5 cut(s) 314, 1040, 1213, 1219, 1372
Asp700I GAANNNNTTC 3 cut(s) 64, 675, 1464
AspLEI GCGC 1 cut(s) 504
AspS9I GGNCC 6 cut(s) 40, 41, 230, 1020, 1327, 1334
AsuC2I CCSGG 1 cut(s) 367
AsuHPI GGTGA 1 cut(s) 284
AvaII GGWCC 3 cut(s) 230, 1020, 1334
BaeGI GKGCMC 4 cut(s) 44, 784, 1231, 1720
BaeI ACNNNNGTAYC 2 cut(s) 924, 957
BalI TGGCCA 2 cut(s) 32, 1263
BanI GGYRCC 1 cut(s) 1241
BanII GRGCYC 2 cut(s) 44, 1055
BbsI GAAGAC 1 cut(s) 57
BbvI GCAGC 5 cut(s) 459, 1040, 1519, 1676, 1712
BccI CCATC 6 cut(s) 617, 977, 1253, 1559, 1597, 1668
BceAI ACGGC 1 cut(s) 1372
BcgI CGANNNNNNTGC 2 cut(s) 1079, 1113
BciT130I CCWGG 2 cut(s) 1325, 1629
BciVI GTATCC 2 cut(s) 435, 728
BclI TGATCA 1 cut(s) 1269
BcnI CCSGG 1 cut(s) 367
BcoDI GTCTC 2 cut(s) 346, 595
BfaI CTAG 3 cut(s) 251, 761, 1256
BfmI CTRYAG 2 cut(s) 110, 1026
BfuI GTATCC 2 cut(s) 435, 728
BisI GCNGC 6 cut(s) 448, 1029, 1508, 1665, 1701, 1712
BlsI GCNGC 6 cut(s) 449, 1030, 1509, 1666, 1702, 1713
Bme1390I CCNGG 3 cut(s) 367, 1325, 1629
Bme18I GGWCC 3 cut(s) 230, 1020, 1334
BmgBI CACGTC 1 cut(s) 1625
BmgT120I GGNCC 6 cut(s) 40, 41, 230, 1020, 1327, 1334
BmiI GGNNCC 2 cut(s) 42, 1243
BmrFI CCNGG 3 cut(s) 367, 1325, 1629
BmrI ACTGGG 2 cut(s) 1225, 1423
BmsI GCATC 4 cut(s) 514, 1093, 1297, 1476
BmuI ACTGGG 2 cut(s) 1225, 1423
BpiI GAAGAC 1 cut(s) 57
BpmI CTGGAG 1 cut(s) 626
Bpu10I CCTNAGC 1 cut(s) 785
BpuEI CTTGAG 2 cut(s) 287, 418
BpuMI CCSGG 1 cut(s) 367
BsaAI YACGTR 1 cut(s) 509
BsaBI GATNNNNATC 1 cut(s) 1614
BsaI GGTCTC 1 cut(s) 346
BsaJI CCNNGG 3 cut(s) 366, 721, 1330
BsaWI WCCGGW 1 cut(s) 1067
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 1160, 1565
Bse1I ACTGG 5 cut(s) 609, 1112, 1231, 1264, 1418
Bse8I GATNNNNATC 1 cut(s) 1614
BseBI CCWGG 2 cut(s) 1325, 1629
BseDI CCNNGG 3 cut(s) 366, 721, 1330
BseGI GGATG 2 cut(s) 628, 1084
BseJI GATNNNNATC 1 cut(s) 1614
BseLI CCNNNNNNNGG 3 cut(s) 75, 1160, 1565
BseMII CTCAG 5 cut(s) 224, 851, 1134, 1467, 1508
BseNI ACTGG 5 cut(s) 609, 1112, 1231, 1264, 1418
BseSI GKGCMC 4 cut(s) 44, 784, 1231, 1720
BseXI GCAGC 5 cut(s) 459, 1040, 1519, 1676, 1712
BseYI CCCAGC 2 cut(s) 875, 1197
BshFI GGCC 6 cut(s) 32, 42, 1263, 1323, 1328, 1539
BshNI GGYRCC 1 cut(s) 1241
BsiSI CCGG 2 cut(s) 366, 1068
BslFI GGGAC 1 cut(s) 415
BslI CCNNNNNNNGG 3 cut(s) 75, 1160, 1565
BsmAI GTCTC 2 cut(s) 346, 595
BsmFI GGGAC 1 cut(s) 415
BsmI GAATGC 1 cut(s) 629
BsnI GGCC 6 cut(s) 32, 42, 1263, 1323, 1328, 1539
Bso31I GGTCTC 1 cut(s) 346
Bsp120I GGGCCC 1 cut(s) 40
Bsp1286I GDGCHC 5 cut(s) 44, 784, 1055, 1231, 1720
Bsp1407I TGTACA 1 cut(s) 558
Bsp143I GATC 8 cut(s) 94, 412, 541, 612, 697, 964, 1269, 1615
BspACI CCGC 4 cut(s) 789, 1088, 1711, 1714
BspANI GGCC 6 cut(s) 32, 42, 1263, 1323, 1328, 1539
BspCNI CTCAG 5 cut(s) 223, 852, 1133, 1466, 1509
BspHI TCATGA 1 cut(s) 1480
BspLI GGNNCC 2 cut(s) 42, 1243
BspMAI CTGCAG 2 cut(s) 114, 1030
BspPI GGATC 3 cut(s) 420, 692, 972
BspT107I GGYRCC 1 cut(s) 1241
BspTNI GGTCTC 1 cut(s) 346
BsrGI TGTACA 1 cut(s) 558
BsrI ACTGG 5 cut(s) 609, 1112, 1231, 1264, 1418
BssECI CCNNGG 3 cut(s) 366, 721, 1330
BssMI GATC 8 cut(s) 94, 412, 541, 612, 697, 964, 1269, 1615
BssT1I CCWWGG 2 cut(s) 721, 1330
Bst2UI CCWGG 2 cut(s) 1325, 1629
Bst4CI ACNGT 4 cut(s) 535, 667, 675, 885
Bst6I CTCTTC 1 cut(s) 1137
BstAPI GCANNNNNTGC 1 cut(s) 1513
BstAUI TGTACA 1 cut(s) 558
BstBAI YACGTR 1 cut(s) 509
BstC8I GCNNGC 4 cut(s) 167, 500, 768, 1716
BstDEI CTNAG 6 cut(s) 210, 785, 860, 1120, 1453, 1517
BstF5I GGATG 2 cut(s) 628, 1084
BstHHI GCGC 1 cut(s) 504
BstKTI GATC 8 cut(s) 97, 415, 544, 615, 700, 967, 1272, 1618
BstMAI GTCTC 2 cut(s) 346, 595
BstMBI GATC 8 cut(s) 94, 412, 541, 612, 697, 964, 1269, 1615
BstMWI GCNNNNNNNGC 2 cut(s) 1513, 1720
BstNI CCWGG 2 cut(s) 1325, 1629
BstNSI RCATGY 3 cut(s) 502, 736, 770
BstSCI CCNGG 3 cut(s) 365, 1323, 1627
BstSFI CTRYAG 2 cut(s) 110, 1026
BstSLI GKGCMC 4 cut(s) 44, 784, 1231, 1720
BstV1I GCAGC 5 cut(s) 459, 1040, 1519, 1676, 1712
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 2 cut(s) 412, 697
BstYI RGATCY 2 cut(s) 412, 697
BsuI GTATCC 2 cut(s) 435, 728
BsuRI GGCC 6 cut(s) 32, 42, 1263, 1323, 1328, 1539
BtrI CACGTC 1 cut(s) 1625
BtsCI GGATG 2 cut(s) 628, 1084
BtsI GCAGTG 1 cut(s) 1023
BtsIMutI CAGTG 3 cut(s) 13, 383, 1023
Cac8I GCNNGC 4 cut(s) 167, 500, 768, 1716
CciI TCATGA 1 cut(s) 1480
CfoI GCGC 1 cut(s) 504
Cfr13I GGNCC 6 cut(s) 40, 41, 230, 1020, 1327, 1334
Csp6I GTAC 2 cut(s) 559, 1419
CviAII CATG 7 cut(s) 185, 326, 499, 733, 767, 1481, 1541
CviQI GTAC 2 cut(s) 559, 1419
DdeI CTNAG 6 cut(s) 210, 785, 860, 1120, 1453, 1517
DpnI GATC 8 cut(s) 96, 414, 543, 614, 699, 966, 1271, 1617
DpnII GATC 8 cut(s) 94, 412, 541, 612, 697, 964, 1269, 1615
EaeI YGGCCR 2 cut(s) 30, 1261
Eam1104I CTCTTC 1 cut(s) 1137
EarI CTCTTC 1 cut(s) 1137
Eco130I CCWWGG 2 cut(s) 721, 1330
Eco24I GRGCYC 2 cut(s) 44, 1055
Eco31I GGTCTC 1 cut(s) 346
Eco47I GGWCC 3 cut(s) 230, 1020, 1334
Eco57I CTGAAG 7 cut(s) 327, 407, 465, 665, 834, 1059, 1510
EcoO109I RGGNCCY 1 cut(s) 41
EcoRI GAATTC 1 cut(s) 1213
EcoRII CCWGG 2 cut(s) 1323, 1627
EcoT14I CCWWGG 2 cut(s) 721, 1330
EcoT22I ATGCAT 1 cut(s) 1576
EcoT38I GRGCYC 2 cut(s) 44, 1055
ErhI CCWWGG 2 cut(s) 721, 1330
FaeI CATG 7 cut(s) 188, 329, 502, 736, 770, 1484, 1544
FaqI GGGAC 1 cut(s) 415
FatI CATG 7 cut(s) 184, 325, 498, 732, 766, 1480, 1540
FauI CCCGC 1 cut(s) 1707
FauNDI CATATG 1 cut(s) 952
FbaI TGATCA 1 cut(s) 1269
FblI GTMKAC 1 cut(s) 1391
Fnu4HI GCNGC 6 cut(s) 448, 1029, 1508, 1665, 1701, 1712
FokI GGATG 2 cut(s) 635, 1071
FriOI GRGCYC 2 cut(s) 44, 1055
Fsp4HI GCNGC 6 cut(s) 448, 1029, 1508, 1665, 1701, 1712
FspAI RTGCGCAY 1 cut(s) 503
FspBI CTAG 3 cut(s) 251, 761, 1256
FspI TGCGCA 1 cut(s) 503
GlaI GCGC 1 cut(s) 503
GluI GCNGC 6 cut(s) 448, 1029, 1508, 1665, 1701, 1712
GsaI CCCAGC 2 cut(s) 879, 1201
GsuI CTGGAG 1 cut(s) 626
HaeIII GGCC 6 cut(s) 32, 42, 1263, 1323, 1328, 1539
HapII CCGG 2 cut(s) 366, 1068
HhaI GCGC 1 cut(s) 504
Hin1II CATG 7 cut(s) 188, 329, 502, 736, 770, 1484, 1544
Hin6I GCGC 1 cut(s) 502
HinP1I GCGC 1 cut(s) 502
HincII GTYRAC 1 cut(s) 1182
HindII GTYRAC 1 cut(s) 1182
HindIII AAGCTT 1 cut(s) 794
HinfI GANTC 4 cut(s) 236, 1129, 1142, 1521
HpaII CCGG 2 cut(s) 366, 1068
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 3 cut(s) 1023, 1182, 1392
Hpy188III TCNNGA 6 cut(s) 228, 483, 654, 812, 1356, 1481
Hpy8I GTNNAC 3 cut(s) 1023, 1182, 1392
HpyAV CCTTC 8 cut(s) 54, 77, 155, 290, 338, 382, 386, 1232
HpyCH4III ACNGT 4 cut(s) 535, 667, 675, 885
HpyCH4IV ACGT 5 cut(s) 22, 340, 508, 594, 1624
HpyCH4V TGCA 9 cut(s) 26, 106, 112, 376, 770, 1028, 1253, 1507, 1574
HpyF10VI GCNNNNNNNGC 2 cut(s) 1513, 1720
HpyF3I CTNAG 6 cut(s) 210, 785, 860, 1120, 1453, 1517
HpySE526I ACGT 5 cut(s) 22, 340, 508, 594, 1624
Hsp92II CATG 7 cut(s) 188, 329, 502, 736, 770, 1484, 1544
HspAI GCGC 1 cut(s) 502
Ksp22I TGATCA 1 cut(s) 1269
Kzo9I GATC 8 cut(s) 94, 412, 541, 612, 697, 964, 1269, 1615
Lsp1109I GCAGC 5 cut(s) 459, 1040, 1519, 1676, 1712
LweI GCATC 4 cut(s) 514, 1093, 1297, 1476
MaeI CTAG 3 cut(s) 251, 761, 1256
MaeII ACGT 5 cut(s) 22, 340, 508, 594, 1624
MaeIII GTNAC 3 cut(s) 220, 379, 1061
MalI GATC 8 cut(s) 96, 414, 543, 614, 699, 966, 1271, 1617
MboI GATC 8 cut(s) 94, 412, 541, 612, 697, 964, 1269, 1615
MfeI CAATTG 1 cut(s) 405
MflI RGATCY 2 cut(s) 412, 697
MhlI GDGCHC 5 cut(s) 44, 784, 1055, 1231, 1720
MlsI TGGCCA 2 cut(s) 32, 1263
MluNI TGGCCA 2 cut(s) 32, 1263
MlyI GAGTC 1 cut(s) 1123
MmeI TCCRAC 2 cut(s) 419, 933
Mox20I TGGCCA 2 cut(s) 32, 1263
Mph1103I ATGCAT 1 cut(s) 1576
MroXI GAANNNNTTC 3 cut(s) 64, 675, 1464
MscI TGGCCA 2 cut(s) 32, 1263
MseI TTAA 6 cut(s) 117, 279, 537, 843, 1398, 1659
Msp20I TGGCCA 2 cut(s) 32, 1263
MspA1I CMGCKG 1 cut(s) 1703
MspI CCGG 2 cut(s) 366, 1068
MspR9I CCNGG 3 cut(s) 367, 1325, 1629
MunI CAATTG 1 cut(s) 405
Mva1269I GAATGC 1 cut(s) 629
MvaI CCWGG 2 cut(s) 1325, 1629
MwoI GCNNNNNNNGC 2 cut(s) 1513, 1720
NciI CCSGG 1 cut(s) 367
NdeI CATATG 1 cut(s) 952
NdeII GATC 8 cut(s) 94, 412, 541, 612, 697, 964, 1269, 1615
NlaIII CATG 7 cut(s) 188, 329, 502, 736, 770, 1484, 1544
NlaIV GGNNCC 2 cut(s) 42, 1243
NmeAIII GCCGAG 1 cut(s) 1451
NmuCI GTSAC 1 cut(s) 379
NsbI TGCGCA 1 cut(s) 503
NsiI ATGCAT 1 cut(s) 1576
NspI RCATGY 3 cut(s) 502, 736, 770
PaeI GCATGC 2 cut(s) 502, 770
PagI TCATGA 1 cut(s) 1480
PctI GAATGC 1 cut(s) 629
PdmI GAANNNNTTC 3 cut(s) 64, 675, 1464
PfeI GAWTC 3 cut(s) 236, 1142, 1521
PkrI GCNGC 6 cut(s) 449, 1030, 1509, 1666, 1702, 1713
PleI GAGTC 1 cut(s) 1123
PpsI GAGTC 1 cut(s) 1123
Ppu21I YACGTR 1 cut(s) 509
Psp6I CCWGG 2 cut(s) 1323, 1627
PspFI CCCAGC 2 cut(s) 875, 1197
PspGI CCWGG 2 cut(s) 1323, 1627
PspN4I GGNNCC 2 cut(s) 42, 1243
PspOMI GGGCCC 1 cut(s) 40
PspPI GGNCC 6 cut(s) 40, 41, 230, 1020, 1327, 1334
PstI CTGCAG 2 cut(s) 114, 1030
PsuI RGATCY 2 cut(s) 412, 697
PvuII CAGCTG 1 cut(s) 1703
RsaI GTAC 2 cut(s) 560, 1420
RsaNI GTAC 2 cut(s) 559, 1419
SaqAI TTAA 6 cut(s) 117, 279, 537, 843, 1398, 1659
SatI GCNGC 6 cut(s) 448, 1029, 1508, 1665, 1701, 1712
Sau3AI GATC 8 cut(s) 94, 412, 541, 612, 697, 964, 1269, 1615
Sau96I GGNCC 6 cut(s) 40, 41, 230, 1020, 1327, 1334
SchI GAGTC 1 cut(s) 1123
ScrFI CCNGG 3 cut(s) 367, 1325, 1629
SduI GDGCHC 5 cut(s) 44, 784, 1055, 1231, 1720
SfaNI GCATC 4 cut(s) 514, 1093, 1297, 1476
SfcI CTRYAG 2 cut(s) 110, 1026
SinI GGWCC 3 cut(s) 230, 1020, 1334
SmlI CTYRAG 2 cut(s) 266, 433
SmoI CTYRAG 2 cut(s) 266, 433
SphI GCATGC 2 cut(s) 502, 770
SsiI CCGC 4 cut(s) 789, 1088, 1711, 1714
SspMI CTAG 3 cut(s) 251, 761, 1256
StyD4I CCNGG 3 cut(s) 365, 1323, 1627
StyI CCWWGG 2 cut(s) 721, 1330
TaaI ACNGT 4 cut(s) 535, 667, 675, 885
TaiI ACGT 5 cut(s) 25, 343, 511, 597, 1627
TaqI TCGA 1 cut(s) 1349
TatI WGTACW 1 cut(s) 558
TauI GCSGC 1 cut(s) 1714
TfiI GAWTC 3 cut(s) 236, 1142, 1521
Tru1I TTAA 6 cut(s) 117, 279, 537, 843, 1398, 1659
Tru9I TTAA 6 cut(s) 117, 279, 537, 843, 1398, 1659
TscAI CASTG 3 cut(s) 13, 390, 1030
TseFI GTSAC 1 cut(s) 379
TseI GCWGC 5 cut(s) 447, 1028, 1507, 1664, 1700
Tsp45I GTSAC 1 cut(s) 379
TspDTI ATGAA 6 cut(s) 173, 218, 300, 309, 969, 1626
TspGWI ACGGA 2 cut(s) 91, 727
TspRI CASTG 3 cut(s) 13, 390, 1030
VpaK11BI GGWCC 3 cut(s) 230, 1020, 1334
XapI RAATTY 5 cut(s) 314, 1040, 1213, 1219, 1372
XceI RCATGY 3 cut(s) 502, 736, 770
XmiI GTMKAC 1 cut(s) 1391
XmnI GAANNNNTTC 3 cut(s) 64, 675, 1464
XspI CTAG 3 cut(s) 251, 761, 1256
Zsp2I ATGCAT 1 cut(s) 1576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.