Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr13

Orthogroup ID

OG0007252

Physical Location & Seq

Genomic Coordinates Reverse (-)

20572214 .. 20578507

Gene Structure 6294 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom13g22770.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1899 bp

ATGAGGCAGACTAGGCTTATGGAGAGGACCAGCTCTATGAGAGAGAAGAGGCAAATGGAAGGGGGAGAGGAGGAGCAACCCGAGCGAAAGCGACCGGCTCTCGCTAGTGTAATTATAGAAGCTCTCAAGGTGGACAGTCTGCAGAAGCTGTGCTCGTCATTGGAACCTATTCTTCGCAGAGTTGTAAGCGAAGAAGTGGAGCGTGCTTTGGCTAAGTTAGGCCCTACGAGAGTTAATGGAAGGTCTTCTCCAAAACGAATTGAAGGTCCAAATGGGCAAAACTTGCAACTAGAGTTTAAGTCCAATTTGGCTCTTCCGATATTCACAGGAGGGAAAGTTGAAGGCGAACAGGGTGCTGCAATTCATGTTGTTTTAGTTGATGCAAACACTGGCCGAGTTCTGACTTCTGGGGCTGAATCATCTGTAAAACTAGATATTGTTGTGCTGGAAGGTGATTTCAACAACGAAGATGATGAAGGCTGGACTCAAGAAGAATTTGATAGCCATGTGGTGAAAGAGCGTGAAGGAAAGAGACCTCTATTGACTGGGGACCTGCAAGTGACCCTCAAAGAAGGGGTAGGAACTCTAGGGGATCTGACCTTCACAGACAACTCAAGTTGGATACGAAGCAGGAAGTTCAGACTTGGATTGAAAGTAGCTTTGGGGTTTTGTGAGGGCATGCGCATACGTGAAGCTAAAACAGAAGCTTTTACTGTTAAAGATCACAGAGGGGAATTGTACAAGAAACACTACCCACCAGCATTAAATGATGAAGTATGGAGATTGGAGAAGATTGGTAAGGATGGGGCATTCCATAAGAGGTTAAACAAAGCAGGAATATGCACAGTTGAAGAGTTTCTTCAGCTTGTGGTCCGAGATTCTCAAAAATTACGGAATATCCTTGGAAGCGGAATGTCAAATAAGATGTGGGATGCTCTTTTAGAACATGCAAAGACATGTGGCTTGAGTGGGAAGCTTTATGTTTATTATCCTGAAGATACAAGAAATGTTGGTGTTGTTTTTAACAACATCTATCAGCTGAGTGGCCTCATCACCGGGGAGCAGTTTCACTCTGCTGATGATCTTTCTGACAGCCAGAAGGTCTATGTAGATGGGCTGGTGAAGAAAGCATATGACAACTGGGAGCAAGTTATTCAGTATGATGCCAAGTCACTTCTAAATTTTAAGCAGATTAAGAGGTTGGCTGCACCCCAAACTGAAATTCAAATGGGCCCAATCAGTTACTCTGAAGCTTCAGATCAACTGCAACTACATCGCCTGCCAAATTCAGTTCACTCAGAGCAGCCTCCATTGGAACCGCTACCAATCGGAGGTTATAATGATGGTCTATCAACAAGATACTTGACCCAGCCACTTGTAAATTCCAATTCTCATACCCAGTTTGATGGCGCCGGATTTGCACTGGATGACCAATTGATCAGCAATTCTCATCTGGCACAAAGCACAAGAAATGACCCCAATACTGTTGGTTTGGCTCTTGGTCCTCCACAATCATCCACATCAGGATTTCAGACTATCAATTCTGCTCAGACATCCACTCTTAATCCTATAGATGACTGGACAGCCAACCGAGATTTTATTTCAGAGGAAGAGATTCGTATTAGAAGTCATGAGATGCTCGAAAATGAGGATATGCAGCACTTGCTCAGAATCTTCAGTATGGGAGGAGGCCATGGCTCAATTGATGTGCCCGATGATGGGTTTGCATTCCCCTCATATGTGCCATCACCAATGCCCAACTATGAGGAAGATCGCACCCGTCCTGGAAAAGCTGTTGTGGGGTGGCTGAAAATTAAGGCAGCAATGAGATGGGGTTTTTTTGTGAGGAAGAAAGCTGCTGAGAGACGGGCACAGATTGTGGAGATAGAGGATGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Calmodulin_bind

Protein Analysis

633

Amino Acids

70.75

Weight (kDa)

5.76

Isoelectric Point (pI)

47.5

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007252)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57580
fragaria_vesca	FvH4_4g09180 FvH4_4g09180 FvH4_4g09180 FvH4_4g09180
malus_domestica	MD13G1267400.v1.1 MD16G1267100.v1.1
prunus_persica	Prupe.1G089100_v2.0.a1 Prupe.1G089100_v2.0.a1 Prupe.1G089100_v2.0.a1 Prupe.1G089100_v2.0.a1 Prupe.1G089100_v2.0.a1 Prupe.1G089100_v2.0.a1
pyrus_communis	pycom13g22770 pycom16g23620
rosa_chinensis	RchiOBHm_Chr4g0403041
rosa_laevigata	RLG00000009029
rosa_multiflora	Rmu_sc0001023.1_g000023
rosa_roxburghii	Rroxscaffold_5G00347730
rosa_rugosa	Rorug04G0040300
rosa_samantha	Rh4AG117600 Rh4BG110100 Rh4CG124400 Rh4DG109500
rosa_wichuraiana	Rw4G009410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1338
Acc16I	TGCGCA	1 cut(s)	683
Acc36I	ACCTGC	1 cut(s)	561
AccB1I	GGYRCC	1 cut(s)	1409
AciI	CCGC	2 cut(s)	909, 1319
AclWI	GGATC	1 cut(s)	600
AcoI	YGGCCR	1 cut(s)	391
AcsI	RAATTY	5 cut(s)	494, 1180, 1221, 1285, 1381
AcuI	CTGAAG	5 cut(s)	845, 1014, 1239, 1269, 1660
AcyI	GRCGYC	1 cut(s)	1410
AfaI	GTAC	1 cut(s)	740
AfiI	CCNNNNNNNGG	3 cut(s)	1331, 1718, 1719
AflIII	ACRYGT	1 cut(s)	956
AgsI	TTSAA	6 cut(s)	263, 341, 460, 652, 851, 1226
AjnI	CCWGG	1 cut(s)	1783
AjuI	GAANNNNNNNTTGG	4 cut(s)	644, 676, 1206, 1238
Alw21I	GWGCWC	1 cut(s)	155
Alw26I	GTCTC	2 cut(s)	526, 1858
AlwI	GGATC	1 cut(s)	600
AlwNI	CAGNNNCTG	1 cut(s)	148
Ama87I	CYCGRG	1 cut(s)	80
AoxI	GGCC	5 cut(s)	220, 391, 1045, 1231, 1690
ApaI	GGGCCC	1 cut(s)	1235
ApeKI	GCWGC	6 cut(s)	356, 1205, 1303, 1657, 1820, 1856
ApoI	RAATTY	5 cut(s)	494, 1180, 1221, 1285, 1381
ArsI	GACNNNNNNTTYG	2 cut(s)	250, 282
Asp700I	GAANNNNTTC	4 cut(s)	168, 244, 855, 1614
AspLEI	GCGC	2 cut(s)	684, 1412
AspS9I	GGNCC	8 cut(s)	27, 221, 266, 550, 871, 1231, 1232, 1502
AsuC2I	CCSGG	1 cut(s)	1057
AsuHPI	GGTGA	5 cut(s)	464, 523, 1045, 1132, 1740
AvaI	CYCGRG	1 cut(s)	80
AvaII	GGWCC	5 cut(s)	27, 266, 550, 871, 1502
BaeGI	GKGCMC	3 cut(s)	1235, 1713, 1873
BanI	GGYRCC	1 cut(s)	1409
BanII	GRGCYC	1 cut(s)	1235
BbsI	GAAGAC	1 cut(s)	237
Bbv12I	GWGCWC	1 cut(s)	155
BbvI	GCAGC	6 cut(s)	343, 1192, 1315, 1669, 1832, 1843
BccI	CCATC	7 cut(s)	797, 1106, 1337, 1400, 1712, 1753, 1824
BcgI	CGANNNNNNTGC	4 cut(s)	335, 369, 1256, 1290
BciT130I	CCWGG	1 cut(s)	1785
BciVI	GTATCC	1 cut(s)	615
BclI	TGATCA	1 cut(s)	1437
BcnI	CCSGG	1 cut(s)	1057
BcoDI	GTCTC	2 cut(s)	526, 1858
BfaI	CTAG	5 cut(s)	12, 105, 290, 431, 587
BfmI	CTRYAG	2 cut(s)	140, 1569
BfoI	RGCGCY	1 cut(s)	1413
BfuAI	ACCTGC	1 cut(s)	561
BfuI	GTATCC	1 cut(s)	615
BisI	GCNGC	6 cut(s)	357, 1206, 1304, 1658, 1821, 1857
BlsI	GCNGC	6 cut(s)	358, 1207, 1305, 1659, 1822, 1858
Bme1390I	CCNGG	2 cut(s)	1057, 1785
Bme18I	GGWCC	5 cut(s)	27, 266, 550, 871, 1502
BmeT110I	CYCGRG	1 cut(s)	80
BmgT120I	GGNCC	8 cut(s)	27, 221, 266, 550, 871, 1231, 1232, 1502
BmiI	GGNNCC	5 cut(s)	165, 551, 1233, 1317, 1411
BmrFI	CCNGG	2 cut(s)	1057, 1785
BmrI	ACTGGG	3 cut(s)	555, 1150, 1393
BmsI	GCATC	4 cut(s)	370, 922, 1153, 1626
BmuI	ACTGGG	3 cut(s)	555, 1150, 1393
BpiI	GAAGAC	1 cut(s)	237
BpuEI	CTTGAG	4 cut(s)	110, 471, 598, 985
BpuMI	CCSGG	1 cut(s)	1057
BsaAI	YACGTR	1 cut(s)	689
BsaHI	GRCGYC	1 cut(s)	1410
BsaI	GGTCTC	1 cut(s)	526
BsaJI	CCNNGG	3 cut(s)	901, 1056, 1693
BsaXI	ACNNNNNCTCC	2 cut(s)	321, 351
Bsc4I	CCNNNNNNNGG	3 cut(s)	1331, 1718, 1719
Bse118I	RCCGGY	1 cut(s)	94
Bse1I	ACTGG	6 cut(s)	394, 550, 1145, 1399, 1428, 1583
Bse3DI	GCAATG	1 cut(s)	1830
BseBI	CCWGG	1 cut(s)	1785
BseDI	CCNNGG	3 cut(s)	901, 1056, 1693
BseGI	GGATG	6 cut(s)	808, 937, 1432, 1514, 1553, 1897
BseLI	CCNNNNNNNGG	3 cut(s)	1331, 1718, 1719
BseMI	GCAATG	1 cut(s)	1830
BseMII	CTCAG	5 cut(s)	1031, 1311, 1562, 1681, 1851
BseNI	ACTGG	6 cut(s)	394, 550, 1145, 1399, 1428, 1583
BseRI	GAGGAG	3 cut(s)	83, 86, 1701
BseSI	GKGCMC	3 cut(s)	1235, 1713, 1873
BseXI	GCAGC	6 cut(s)	343, 1192, 1315, 1669, 1832, 1843
BseYI	CCCAGC	1 cut(s)	1368
BsgI	GTGCAG	1 cut(s)	1191
Bsh1285I	CGRYCG	1 cut(s)	95
BshFI	GGCC	5 cut(s)	222, 393, 1047, 1233, 1692
BshNI	GGYRCC	1 cut(s)	1409
BsiEI	CGRYCG	1 cut(s)	95
BsiHKAI	GWGCWC	1 cut(s)	155
BsiHKCI	CYCGRG	1 cut(s)	80
BsiSI	CCGG	3 cut(s)	95, 1056, 1413
BslFI	GGGAC	1 cut(s)	563
BslI	CCNNNNNNNGG	3 cut(s)	1331, 1718, 1719
BsmAI	GTCTC	2 cut(s)	526, 1858
BsmBI	CGTCTC	1 cut(s)	1858
BsmFI	GGGAC	1 cut(s)	563
BsmI	GAATGC	2 cut(s)	809, 1727
BsnI	GGCC	5 cut(s)	222, 393, 1047, 1233, 1692
Bso31I	GGTCTC	1 cut(s)	526
BsoBI	CYCGRG	1 cut(s)	80
Bsp120I	GGGCCC	1 cut(s)	1231
Bsp1286I	GDGCHC	4 cut(s)	155, 1235, 1713, 1873
Bsp1407I	TGTACA	1 cut(s)	738
Bsp143I	GATC	6 cut(s)	592, 721, 1081, 1258, 1437, 1771
Bsp19I	CCATGG	1 cut(s)	1693
BspACI	CCGC	2 cut(s)	909, 1319
BspANI	GGCC	5 cut(s)	222, 393, 1047, 1233, 1692
BspCNI	CTCAG	5 cut(s)	1032, 1310, 1561, 1680, 1852
BspHI	TCATGA	1 cut(s)	1630
BspLI	GGNNCC	5 cut(s)	165, 551, 1233, 1317, 1411
BspMAI	CTGCAG	1 cut(s)	144
BspMI	ACCTGC	1 cut(s)	561
BspPI	GGATC	1 cut(s)	600
BspQI	GCTCTTC	1 cut(s)	318
BspT107I	GGYRCC	1 cut(s)	1409
BspTNI	GGTCTC	1 cut(s)	526
BsrDI	GCAATG	1 cut(s)	1830
BsrFI	RCCGGY	1 cut(s)	94
BsrGI	TGTACA	1 cut(s)	738
BsrI	ACTGG	6 cut(s)	394, 550, 1145, 1399, 1428, 1583
BssAI	RCCGGY	1 cut(s)	94
BssECI	CCNNGG	3 cut(s)	901, 1056, 1693
BssMI	GATC	6 cut(s)	592, 721, 1081, 1258, 1437, 1771
BssNI	GRCGYC	1 cut(s)	1410
BssT1I	CCWWGG	2 cut(s)	901, 1693
Bst2UI	CCWGG	1 cut(s)	1785
Bst4CI	ACNGT	4 cut(s)	137, 715, 847, 1486
Bst6I	CTCTTC	4 cut(s)	41, 318, 846, 1605
BstACI	GRCGYC	1 cut(s)	1410
BstAPI	GCANNNNNTGC	2 cut(s)	283, 1663
BstAUI	TGTACA	1 cut(s)	738
BstBAI	YACGTR	1 cut(s)	689
BstC8I	GCNNGC	3 cut(s)	204, 680, 1280
BstDEI	CTNAG	6 cut(s)	213, 1040, 1297, 1548, 1667, 1860
BstDSI	CCRYGG	1 cut(s)	1693
BstF5I	GGATG	6 cut(s)	808, 937, 1432, 1514, 1553, 1897
BstH2I	RGCGCY	1 cut(s)	1413
BstHHI	GCGC	2 cut(s)	684, 1412
BstKTI	GATC	6 cut(s)	595, 724, 1084, 1261, 1440, 1774
BstMAI	GTCTC	2 cut(s)	526, 1858
BstMBI	GATC	6 cut(s)	592, 721, 1081, 1258, 1437, 1771
BstMCI	CGRYCG	1 cut(s)	95
BstMWI	GCNNNNNNNGC	5 cut(s)	13, 82, 283, 1418, 1663
BstNI	CCWGG	1 cut(s)	1785
BstNSI	RCATGY	3 cut(s)	682, 950, 960
BstSCI	CCNGG	2 cut(s)	1055, 1783
BstSFI	CTRYAG	2 cut(s)	140, 1569
BstSLI	GKGCMC	3 cut(s)	1235, 1713, 1873
BstV1I	GCAGC	6 cut(s)	343, 1192, 1315, 1669, 1832, 1843
BstV2I	GAAGAC	1 cut(s)	237
BstX2I	RGATCY	1 cut(s)	592
BstXI	CCANNNNNNTGG	1 cut(s)	1406
BstYI	RGATCY	1 cut(s)	592
BsuI	GTATCC	1 cut(s)	615
BsuRI	GGCC	5 cut(s)	222, 393, 1047, 1233, 1692
BtgI	CCRYGG	1 cut(s)	1693
BtgZI	GCGATG	1 cut(s)	1259
BtsCI	GGATG	6 cut(s)	808, 937, 1432, 1514, 1553, 1897
BtsIMutI	CAGTG	2 cut(s)	387, 1421
BveI	ACCTGC	1 cut(s)	561
Cac8I	GCNNGC	3 cut(s)	204, 680, 1280
CaiI	CAGNNNCTG	1 cut(s)	148
CciI	TCATGA	1 cut(s)	1630
CfoI	GCGC	2 cut(s)	684, 1412
Cfr10I	RCCGGY	1 cut(s)	94
Cfr13I	GGNCC	8 cut(s)	27, 221, 266, 550, 871, 1231, 1232, 1502
Csp6I	GTAC	1 cut(s)	739
CviAII	CATG	7 cut(s)	365, 506, 679, 947, 957, 1631, 1694
CviQI	GTAC	1 cut(s)	739
DdeI	CTNAG	6 cut(s)	213, 1040, 1297, 1548, 1667, 1860
DinI	GGCGCC	1 cut(s)	1411
DpnI	GATC	6 cut(s)	594, 723, 1083, 1260, 1439, 1773
DpnII	GATC	6 cut(s)	592, 721, 1081, 1258, 1437, 1771
EaeI	YGGCCR	1 cut(s)	391
Eam1104I	CTCTTC	4 cut(s)	41, 318, 846, 1605
EarI	CTCTTC	4 cut(s)	41, 318, 846, 1605
Eco130I	CCWWGG	2 cut(s)	901, 1693
Eco24I	GRGCYC	1 cut(s)	1235
Eco31I	GGTCTC	1 cut(s)	526
Eco47I	GGWCC	5 cut(s)	27, 266, 550, 871, 1502
Eco57I	CTGAAG	5 cut(s)	845, 1014, 1239, 1269, 1660
Eco88I	CYCGRG	1 cut(s)	80
EcoO109I	RGGNCCY	2 cut(s)	221, 550
EcoRII	CCWGG	1 cut(s)	1783
EcoT14I	CCWWGG	2 cut(s)	901, 1693
EcoT38I	GRGCYC	1 cut(s)	1235
EgeI	GGCGCC	1 cut(s)	1411
EheI	GGCGCC	1 cut(s)	1411
ErhI	CCWWGG	2 cut(s)	901, 1693
Esp3I	CGTCTC	1 cut(s)	1858
FaeI	CATG	7 cut(s)	368, 509, 682, 950, 960, 1634, 1697
FalI	AAGNNNNNCTT	2 cut(s)	843, 875
FaqI	GGGAC	1 cut(s)	563
FatI	CATG	7 cut(s)	364, 505, 678, 946, 956, 1630, 1693
FauNDI	CATATG	2 cut(s)	1132, 1738
FbaI	TGATCA	1 cut(s)	1437
Fnu4HI	GCNGC	6 cut(s)	357, 1206, 1304, 1658, 1821, 1857
FokI	GGATG	5 cut(s)	815, 944, 1439, 1501, 1540
FriOI	GRGCYC	1 cut(s)	1235
Fsp4HI	GCNGC	6 cut(s)	357, 1206, 1304, 1658, 1821, 1857
FspAI	RTGCGCAY	1 cut(s)	683
FspBI	CTAG	5 cut(s)	12, 105, 290, 431, 587
FspI	TGCGCA	1 cut(s)	683
GlaI	GCGC	2 cut(s)	683, 1411
GluI	GCNGC	6 cut(s)	357, 1206, 1304, 1658, 1821, 1857
GsaI	CCCAGC	1 cut(s)	1372
HaeII	RGCGCY	1 cut(s)	1413
HaeIII	GGCC	5 cut(s)	222, 393, 1047, 1233, 1692
HapII	CCGG	3 cut(s)	95, 1056, 1413
HhaI	GCGC	2 cut(s)	684, 1412
Hin1I	GRCGYC	1 cut(s)	1410
Hin1II	CATG	7 cut(s)	368, 509, 682, 950, 960, 1634, 1697
Hin6I	GCGC	2 cut(s)	682, 1410
HinP1I	GCGC	2 cut(s)	682, 1410
HindIII	AAGCTT	3 cut(s)	705, 974, 1251
HinfI	GANTC	5 cut(s)	416, 484, 878, 1615, 1671
HpaII	CCGG	3 cut(s)	95, 1056, 1413
HphI	GGTGA	5 cut(s)	464, 523, 1045, 1132, 1740
Hpy166II	GTNNAC	2 cut(s)	133, 1294
Hpy188III	TCNNGA	4 cut(s)	488, 992, 1524, 1631
Hpy8I	GTNNAC	2 cut(s)	133, 1294
HpyCH4III	ACNGT	4 cut(s)	137, 715, 847, 1486
HpyCH4IV	ACGT	1 cut(s)	688
HpyF10VI	GCNNNNNNNGC	5 cut(s)	13, 82, 283, 1418, 1663
HpyF3I	CTNAG	6 cut(s)	213, 1040, 1297, 1548, 1667, 1860
HpySE526I	ACGT	1 cut(s)	688
Hsp92I	GRCGYC	1 cut(s)	1410
Hsp92II	CATG	7 cut(s)	368, 509, 682, 950, 960, 1634, 1697
HspAI	GCGC	2 cut(s)	682, 1410
KasI	GGCGCC	1 cut(s)	1409
Ksp22I	TGATCA	1 cut(s)	1437
Kzo9I	GATC	6 cut(s)	592, 721, 1081, 1258, 1437, 1771
LguI	GCTCTTC	1 cut(s)	318
LmnI	GCTCC	4 cut(s)	73, 199, 1060, 1144
Lsp1109I	GCAGC	6 cut(s)	343, 1192, 1315, 1669, 1832, 1843
LweI	GCATC	4 cut(s)	370, 922, 1153, 1626
MaeI	CTAG	5 cut(s)	12, 105, 290, 431, 587
MaeII	ACGT	1 cut(s)	688
MaeIII	GTNAC	3 cut(s)	559, 1170, 1241
MalI	GATC	6 cut(s)	594, 723, 1083, 1260, 1439, 1773
MboI	GATC	6 cut(s)	592, 721, 1081, 1258, 1437, 1771
MfeI	CAATTG	2 cut(s)	1433, 1701
MflI	RGATCY	1 cut(s)	592
MhlI	GDGCHC	4 cut(s)	155, 1235, 1713, 1873
Mly113I	GGCGCC	1 cut(s)	1410
MlyI	GAGTC	1 cut(s)	478
MmeI	TCCRAC	1 cut(s)	599
MroXI	GAANNNNTTC	4 cut(s)	168, 244, 855, 1614
MspA1I	CMGCKG	1 cut(s)	1039
MspI	CCGG	3 cut(s)	95, 1056, 1413
MspR9I	CCNGG	2 cut(s)	1057, 1785
MunI	CAATTG	2 cut(s)	1433, 1701
Mva1269I	GAATGC	2 cut(s)	809, 1727
MvaI	CCWGG	1 cut(s)	1785
MwoI	GCNNNNNNNGC	5 cut(s)	13, 82, 283, 1418, 1663
NarI	GGCGCC	1 cut(s)	1410
NciI	CCSGG	1 cut(s)	1057
NcoI	CCATGG	1 cut(s)	1693
NdeI	CATATG	2 cut(s)	1132, 1738
NdeII	GATC	6 cut(s)	592, 721, 1081, 1258, 1437, 1771
NlaIII	CATG	7 cut(s)	368, 509, 682, 950, 960, 1634, 1697
NlaIV	GGNNCC	5 cut(s)	165, 551, 1233, 1317, 1411
NmeAIII	GCCGAG	1 cut(s)	419
NmuCI	GTSAC	2 cut(s)	559, 1170
NsbI	TGCGCA	1 cut(s)	683
NspI	RCATGY	3 cut(s)	682, 950, 960
PaeI	GCATGC	1 cut(s)	682
PagI	TCATGA	1 cut(s)	1630
PciI	ACATGT	1 cut(s)	956
PciSI	GCTCTTC	1 cut(s)	318
PctI	GAATGC	2 cut(s)	809, 1727
PdmI	GAANNNNTTC	4 cut(s)	168, 244, 855, 1614
PfeI	GAWTC	4 cut(s)	416, 878, 1615, 1671
PfoI	TCCNGGA	1 cut(s)	1783
PkrI	GCNGC	6 cut(s)	358, 1207, 1305, 1659, 1822, 1858
PleI	GAGTC	1 cut(s)	478
PluTI	GGCGCC	1 cut(s)	1413
PpsI	GAGTC	1 cut(s)	478
Ppu21I	YACGTR	1 cut(s)	689
PpuMI	RGGWCCY	1 cut(s)	550
PscI	ACATGT	1 cut(s)	956
PsiI	TTATAA	1 cut(s)	1338
Psp5II	RGGWCCY	1 cut(s)	550
Psp6I	CCWGG	1 cut(s)	1783
PspFI	CCCAGC	1 cut(s)	1368
PspGI	CCWGG	1 cut(s)	1783
PspN4I	GGNNCC	5 cut(s)	165, 551, 1233, 1317, 1411
PspOMI	GGGCCC	1 cut(s)	1231
PspPI	GGNCC	8 cut(s)	27, 221, 266, 550, 871, 1231, 1232, 1502
PspPPI	RGGWCCY	1 cut(s)	550
PstI	CTGCAG	1 cut(s)	144
PstNI	CAGNNNCTG	1 cut(s)	148
PsuI	RGATCY	1 cut(s)	592
PvuII	CAGCTG	1 cut(s)	1039
RsaI	GTAC	1 cut(s)	740
RsaNI	GTAC	1 cut(s)	739
SapI	GCTCTTC	1 cut(s)	318
SatI	GCNGC	6 cut(s)	357, 1206, 1304, 1658, 1821, 1857
Sau3AI	GATC	6 cut(s)	592, 721, 1081, 1258, 1437, 1771
Sau96I	GGNCC	8 cut(s)	27, 221, 266, 550, 871, 1231, 1232, 1502
SchI	GAGTC	1 cut(s)	478
ScrFI	CCNGG	2 cut(s)	1057, 1785
SduI	GDGCHC	4 cut(s)	155, 1235, 1713, 1873
SfaNI	GCATC	4 cut(s)	370, 922, 1153, 1626
SfcI	CTRYAG	2 cut(s)	140, 1569
SfoI	GGCGCC	1 cut(s)	1411
SinI	GGWCC	5 cut(s)	27, 266, 550, 871, 1502
SmlI	CTYRAG	4 cut(s)	125, 486, 613, 964
SmoI	CTYRAG	4 cut(s)	125, 486, 613, 964
SphI	GCATGC	1 cut(s)	682
SsiI	CCGC	2 cut(s)	909, 1319
SspDI	GGCGCC	1 cut(s)	1409
SspMI	CTAG	5 cut(s)	12, 105, 290, 431, 587
StyD4I	CCNGG	2 cut(s)	1055, 1783
StyI	CCWWGG	2 cut(s)	901, 1693
TaaI	ACNGT	4 cut(s)	137, 715, 847, 1486
TaiI	ACGT	1 cut(s)	691
TaqI	TCGA	1 cut(s)	1641
TatI	WGTACW	1 cut(s)	738
TfiI	GAWTC	4 cut(s)	416, 878, 1615, 1671
TscAI	CASTG	2 cut(s)	394, 1428
TseFI	GTSAC	2 cut(s)	559, 1170
TseI	GCWGC	6 cut(s)	356, 1205, 1303, 1657, 1820, 1856
Tsp45I	GTSAC	2 cut(s)	559, 1170
TspDTI	ATGAA	3 cut(s)	353, 489, 786
TspGWI	ACGGA	1 cut(s)	907
TspRI	CASTG	2 cut(s)	394, 1428
VpaK11BI	GGWCC	5 cut(s)	27, 266, 550, 871, 1502
XapI	RAATTY	5 cut(s)	494, 1180, 1221, 1285, 1381
XceI	RCATGY	3 cut(s)	682, 950, 960
XmnI	GAANNNNTTC	4 cut(s)	168, 244, 855, 1614
XspI	CTAG	5 cut(s)	12, 105, 290, 431, 587

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.