Rh4AG117600

Calmodulin binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0007252
Physical Location & Seq
Reverse (-)
25303598 .. 25311084
7487 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG117600.1

Sequence Viewer

Length: 1542 bp
ATGAACACTCAGCGTGTTGTAACATCTGGACCTGAATCCTCTATAAAACTAGATATTGTTGTGCTTGAGGGTGATTTTAACAATGAAGATGATGAAGGCTGGACTGAAGAAGAATTTGAAAGCCATGTGGTCAAAGAGCGTGAAGGAAAGAGACCTCTTTTGACCGGGGAATTGCAAGTGACACTGAAGGAAGGGGTAGGGACAATTGGGGATCTGACTTTTACAGATAACTCAAGTTGGATACGCAGCAGGAAGTTTAGGCTTGGATTGAAAGTTGCTTCAGGATTTTGTGAGGGCATGCGCATACGTGAAGCGAAAACAGATGCTTTTACTGTTAAAGATCACAGAGGGGAATTGTACAAGAAACATTACCCACCAGCACTAAATGATGACGTATGGAGACTGGAGAAGATCGGAAAGGATGGGGCATTCCATAAGAGGCTAAACAATGCAGGAATACACACAGTTGAACAGTTTCTTCAGCTTGTGGTCAAAGATCCTCAAAAATTACGGAGTATCCTTGGAAGTGGCATGTCAAATAAGATGTGGGAAGCTCTCCTAGAGCATGCAAAGACTTGTGCCCTTAGCGGAAAGCTTTATGTTTATTATCCTGAAGATACAAGAAATGTTGGTGTTGCTTTTAACAACATCTATGAGCTGAGTGGCTTGATTGCTGGGGAACAGTATCATTCTGCCGATACTCTTTCTGACAGCCAGAAGCTCTATGTAGATACGCTTGTGAAGAAAGCATATGAAAATTGGGATCAGGTTGTTGAGTATGATGGTAAATCACTTCTGAACACCAAGCCAAATAAGAGGTCAACTGCAGCCCGAAACGAATTTCAAATGGGCTCAATCAGTTACTCCAGTTCTTTAGACCAGCAACCGCAACTACCTCGCCTGACAAATCCAGTTCCCTCAGAACAGACTCTTCTATCTGATTCAGCCCTACCGATTGGAGGGTATAATGATAATCTGTCAACAAGGTATATGACCCAGCAACAAATTGTGAATTCAAATTCCCGTGCCCAGTTTGAAGGCACCTCATTTGCACTAGATGGCCAGTTGATCAACAGCTCTCATCAGCCTCAAAGCATAACAAATGATGCCAATGCTGTTGGCCTGGCCCTTGGTCCTCCACAATCATCGGCATCAGGATTTCAGGCTATAAATTCTTCTGTTCAGCCGTCTACCCTTAATCCTTTAGATGACTGGGGAACAAGCCGAGAAAAAGGAGTTGATGACTTCTTCTCTGAAGAAGATATTCGCATTAGAAGTCATGAGATGCTTGAAAATGAGGATATGCAGCACTTGCTCAGAATCTTCAGTATGGGAGGCCATGCCTCTATTGATATGCCTGATGATGGATATGCATACCCATATATGCCATCTCCAATGCCCAACTTTGATGAAGATCGCACACGTCCTGGCAAAGCTGTTGTTGGGTGGCTGAAAATTAAGGCAGCTATGAGATGGGGTTTCTTTGTCAGAAAGAAAGCAGCTGAAAGGCGGCGGGCACAGCTTGTGGAGTTAGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

513

Amino Acids

57.61

Weight (kDa)

5.47

Isoelectric Point (pI)

43.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 2 - 115 7.3e-51 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 127 - 193 9.1e-34 Calmodulin binding protein central domain
Calmod_bind_C PF20452 198 - 260 2e-25 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 302
AccB1I GGYRCC 1 cut(s) 1040
AccI GTMKAC 1 cut(s) 1190
AciI CCGC 4 cut(s) 588, 887, 1510, 1513
AclWI GGATC 3 cut(s) 219, 491, 771
AcoI YGGCCR 1 cut(s) 1060
AcsI RAATTY 5 cut(s) 113, 839, 1012, 1018, 1171
AcuI CTGAAG 7 cut(s) 126, 206, 264, 464, 633, 1275, 1309
AfaI GTAC 1 cut(s) 359
AfiI CCNNNNNNNGG 2 cut(s) 959, 1364
AflIII ACRYGT 1 cut(s) 1421
AgsI TTSAA 7 cut(s) 119, 271, 470, 845, 1017, 1037, 1292
AjiI CACGTC 1 cut(s) 1424
AjnI CCWGG 2 cut(s) 1122, 1426
Alw26I GTCTC 2 cut(s) 145, 394
AlwI GGATC 3 cut(s) 219, 491, 771
AoxI GGCC 4 cut(s) 1060, 1120, 1125, 1336
ApeKI GCWGC 5 cut(s) 246, 827, 1306, 1463, 1499
ApoI RAATTY 5 cut(s) 113, 839, 1012, 1018, 1171
ArsI GACNNNNNNTTYG 2 cut(s) 803, 835
Asp700I GAANNNNTTC 2 cut(s) 474, 1263
AspLEI GCGC 1 cut(s) 303
AspS9I GGNCC 3 cut(s) 29, 1126, 1133
AsuC2I CCSGG 1 cut(s) 166
AsuHPI GGTGA 1 cut(s) 83
AvaII GGWCC 2 cut(s) 29, 1133
BaeGI GKGCMC 3 cut(s) 583, 1030, 1519
BaeI ACNNNNGTAYC 2 cut(s) 723, 756
BalI TGGCCA 1 cut(s) 1062
BanI GGYRCC 1 cut(s) 1040
BanII GRGCYC 1 cut(s) 854
BbvI GCAGC 5 cut(s) 258, 839, 1318, 1475, 1511
BccI CCATC 6 cut(s) 416, 776, 1052, 1358, 1396, 1467
BceAI ACGGC 1 cut(s) 1171
BcgI CGANNNNNNTGC 2 cut(s) 878, 912
BciT130I CCWGG 2 cut(s) 1124, 1428
BciVI GTATCC 2 cut(s) 234, 527
BclI TGATCA 1 cut(s) 1068
BcnI CCSGG 1 cut(s) 166
BcoDI GTCTC 2 cut(s) 145, 394
BfaI CTAG 3 cut(s) 50, 560, 1055
BfmI CTRYAG 1 cut(s) 825
BfuI GTATCC 2 cut(s) 234, 527
BisI GCNGC 6 cut(s) 247, 828, 1307, 1464, 1500, 1511
BlsI GCNGC 6 cut(s) 248, 829, 1308, 1465, 1501, 1512
Bme1390I CCNGG 3 cut(s) 166, 1124, 1428
Bme18I GGWCC 2 cut(s) 29, 1133
BmgBI CACGTC 1 cut(s) 1424
BmgT120I GGNCC 3 cut(s) 29, 1126, 1133
BmiI GGNNCC 1 cut(s) 1042
BmrFI CCNGG 3 cut(s) 166, 1124, 1428
BmrI ACTGGG 2 cut(s) 1024, 1222
BmsI GCATC 4 cut(s) 313, 1096, 1160, 1275
BmuI ACTGGG 2 cut(s) 1024, 1222
BpmI CTGGAG 2 cut(s) 425, 850
Bpu10I CCTNAGC 1 cut(s) 584
BpuEI CTTGAG 2 cut(s) 86, 217
BpuMI CCSGG 1 cut(s) 166
BsaAI YACGTR 1 cut(s) 308
BsaBI GATNNNNATC 1 cut(s) 1413
BsaI GGTCTC 1 cut(s) 145
BsaJI CCNNGG 3 cut(s) 165, 520, 1129
Bsc4I CCNNNNNNNGG 2 cut(s) 959, 1364
Bse1I ACTGG 6 cut(s) 408, 867, 911, 1030, 1063, 1217
Bse8I GATNNNNATC 1 cut(s) 1413
BseBI CCWGG 2 cut(s) 1124, 1428
BseDI CCNNGG 3 cut(s) 165, 520, 1129
BseGI GGATG 1 cut(s) 427
BseJI GATNNNNATC 1 cut(s) 1413
BseLI CCNNNNNNNGG 2 cut(s) 959, 1364
BseMII CTCAG 4 cut(s) 23, 650, 933, 1330
BseNI ACTGG 6 cut(s) 408, 867, 911, 1030, 1063, 1217
BseSI GKGCMC 3 cut(s) 583, 1030, 1519
BseXI GCAGC 5 cut(s) 258, 839, 1318, 1475, 1511
BseYI CCCAGC 2 cut(s) 674, 996
BshFI GGCC 4 cut(s) 1062, 1122, 1127, 1338
BshNI GGYRCC 1 cut(s) 1040
BsiSI CCGG 1 cut(s) 165
BslFI GGGAC 1 cut(s) 214
BslI CCNNNNNNNGG 2 cut(s) 959, 1364
BsmAI GTCTC 2 cut(s) 145, 394
BsmFI GGGAC 1 cut(s) 214
BsmI GAATGC 1 cut(s) 428
BsnI GGCC 4 cut(s) 1062, 1122, 1127, 1338
Bso31I GGTCTC 1 cut(s) 145
Bsp1286I GDGCHC 4 cut(s) 583, 854, 1030, 1519
Bsp1407I TGTACA 1 cut(s) 357
Bsp143I GATC 7 cut(s) 211, 340, 411, 496, 763, 1068, 1414
BspACI CCGC 4 cut(s) 588, 887, 1510, 1513
BspANI GGCC 4 cut(s) 1062, 1122, 1127, 1338
BspCNI CTCAG 4 cut(s) 22, 651, 932, 1329
BspHI TCATGA 1 cut(s) 1279
BspLI GGNNCC 1 cut(s) 1042
BspMAI CTGCAG 1 cut(s) 829
BspPI GGATC 3 cut(s) 219, 491, 771
BspT107I GGYRCC 1 cut(s) 1040
BspTNI GGTCTC 1 cut(s) 145
BsrGI TGTACA 1 cut(s) 357
BsrI ACTGG 6 cut(s) 408, 867, 911, 1030, 1063, 1217
BssECI CCNNGG 3 cut(s) 165, 520, 1129
BssMI GATC 7 cut(s) 211, 340, 411, 496, 763, 1068, 1414
BssT1I CCWWGG 2 cut(s) 520, 1129
Bst2UI CCWGG 2 cut(s) 1124, 1428
Bst4CI ACNGT 4 cut(s) 334, 466, 474, 684
Bst6I CTCTTC 1 cut(s) 936
BstAPI GCANNNNNTGC 1 cut(s) 1312
BstAUI TGTACA 1 cut(s) 357
BstBAI YACGTR 1 cut(s) 308
BstC8I GCNNGC 3 cut(s) 299, 567, 1515
BstDEI CTNAG 5 cut(s) 9, 584, 659, 919, 1316
BstF5I GGATG 1 cut(s) 427
BstHHI GCGC 1 cut(s) 303
BstKTI GATC 7 cut(s) 214, 343, 414, 499, 766, 1071, 1417
BstMAI GTCTC 2 cut(s) 145, 394
BstMBI GATC 7 cut(s) 211, 340, 411, 496, 763, 1068, 1414
BstMWI GCNNNNNNNGC 2 cut(s) 1312, 1519
BstNI CCWGG 2 cut(s) 1124, 1428
BstNSI RCATGY 3 cut(s) 301, 535, 569
BstSCI CCNGG 3 cut(s) 164, 1122, 1426
BstSFI CTRYAG 1 cut(s) 825
BstSLI GKGCMC 3 cut(s) 583, 1030, 1519
BstV1I GCAGC 5 cut(s) 258, 839, 1318, 1475, 1511
BstX2I RGATCY 2 cut(s) 211, 496
BstYI RGATCY 2 cut(s) 211, 496
BsuI GTATCC 2 cut(s) 234, 527
BsuRI GGCC 4 cut(s) 1062, 1122, 1127, 1338
BtrI CACGTC 1 cut(s) 1424
BtsCI GGATG 1 cut(s) 427
BtsIMutI CAGTG 1 cut(s) 182
Cac8I GCNNGC 3 cut(s) 299, 567, 1515
CciI TCATGA 1 cut(s) 1279
CfoI GCGC 1 cut(s) 303
Cfr13I GGNCC 3 cut(s) 29, 1126, 1133
Csp6I GTAC 1 cut(s) 358
CviAII CATG 6 cut(s) 125, 298, 532, 566, 1280, 1340
CviQI GTAC 1 cut(s) 358
DdeI CTNAG 5 cut(s) 9, 584, 659, 919, 1316
DpnI GATC 7 cut(s) 213, 342, 413, 498, 765, 1070, 1416
DpnII GATC 7 cut(s) 211, 340, 411, 496, 763, 1068, 1414
EaeI YGGCCR 1 cut(s) 1060
Eam1104I CTCTTC 1 cut(s) 936
EarI CTCTTC 1 cut(s) 936
Eco130I CCWWGG 2 cut(s) 520, 1129
Eco24I GRGCYC 1 cut(s) 854
Eco31I GGTCTC 1 cut(s) 145
Eco47I GGWCC 2 cut(s) 29, 1133
Eco57I CTGAAG 7 cut(s) 126, 206, 264, 464, 633, 1275, 1309
EcoRI GAATTC 1 cut(s) 1012
EcoRII CCWGG 2 cut(s) 1122, 1426
EcoT14I CCWWGG 2 cut(s) 520, 1129
EcoT22I ATGCAT 1 cut(s) 1375
EcoT38I GRGCYC 1 cut(s) 854
ErhI CCWWGG 2 cut(s) 520, 1129
FaeI CATG 6 cut(s) 128, 301, 535, 569, 1283, 1343
FaqI GGGAC 1 cut(s) 214
FatI CATG 6 cut(s) 124, 297, 531, 565, 1279, 1339
FauI CCCGC 1 cut(s) 1506
FauNDI CATATG 1 cut(s) 751
FbaI TGATCA 1 cut(s) 1068
FblI GTMKAC 1 cut(s) 1190
Fnu4HI GCNGC 6 cut(s) 247, 828, 1307, 1464, 1500, 1511
FokI GGATG 1 cut(s) 434
FriOI GRGCYC 1 cut(s) 854
Fsp4HI GCNGC 6 cut(s) 247, 828, 1307, 1464, 1500, 1511
FspAI RTGCGCAY 1 cut(s) 302
FspBI CTAG 3 cut(s) 50, 560, 1055
FspI TGCGCA 1 cut(s) 302
GlaI GCGC 1 cut(s) 302
GluI GCNGC 6 cut(s) 247, 828, 1307, 1464, 1500, 1511
GsaI CCCAGC 2 cut(s) 678, 1000
GsuI CTGGAG 2 cut(s) 425, 850
HaeIII GGCC 4 cut(s) 1062, 1122, 1127, 1338
HapII CCGG 1 cut(s) 165
HhaI GCGC 1 cut(s) 303
Hin1II CATG 6 cut(s) 128, 301, 535, 569, 1283, 1343
Hin6I GCGC 1 cut(s) 301
HinP1I GCGC 1 cut(s) 301
HincII GTYRAC 2 cut(s) 822, 981
HindII GTYRAC 2 cut(s) 822, 981
HindIII AAGCTT 1 cut(s) 593
HinfI GANTC 4 cut(s) 35, 928, 941, 1320
HpaII CCGG 1 cut(s) 165
HphI GGTGA 1 cut(s) 83
Hpy166II GTNNAC 3 cut(s) 822, 981, 1191
Hpy188I TCNGA 9 cut(s) 216, 416, 709, 798, 922, 940, 1255, 1319, 1490
Hpy188III TCNNGA 5 cut(s) 27, 282, 611, 1155, 1280
Hpy8I GTNNAC 3 cut(s) 822, 981, 1191
HpyAV CCTTC 5 cut(s) 89, 137, 181, 185, 1031
HpyCH4III ACNGT 4 cut(s) 334, 466, 474, 684
HpyCH4IV ACGT 3 cut(s) 307, 393, 1423
HpyCH4V TGCA 7 cut(s) 175, 452, 569, 827, 1052, 1306, 1373
HpyF10VI GCNNNNNNNGC 2 cut(s) 1312, 1519
HpyF3I CTNAG 5 cut(s) 9, 584, 659, 919, 1316
HpySE526I ACGT 3 cut(s) 307, 393, 1423
Hsp92II CATG 6 cut(s) 128, 301, 535, 569, 1283, 1343
HspAI GCGC 1 cut(s) 301
Ksp22I TGATCA 1 cut(s) 1068
Kzo9I GATC 7 cut(s) 211, 340, 411, 496, 763, 1068, 1414
Lsp1109I GCAGC 5 cut(s) 258, 839, 1318, 1475, 1511
LweI GCATC 4 cut(s) 313, 1096, 1160, 1275
MaeI CTAG 3 cut(s) 50, 560, 1055
MaeII ACGT 3 cut(s) 307, 393, 1423
MaeIII GTNAC 3 cut(s) 19, 178, 860
MalI GATC 7 cut(s) 213, 342, 413, 498, 765, 1070, 1416
MboI GATC 7 cut(s) 211, 340, 411, 496, 763, 1068, 1414
MfeI CAATTG 1 cut(s) 204
MflI RGATCY 2 cut(s) 211, 496
MhlI GDGCHC 4 cut(s) 583, 854, 1030, 1519
MlsI TGGCCA 1 cut(s) 1062
MluNI TGGCCA 1 cut(s) 1062
MlyI GAGTC 1 cut(s) 922
MmeI TCCRAC 1 cut(s) 218
Mox20I TGGCCA 1 cut(s) 1062
Mph1103I ATGCAT 1 cut(s) 1375
MroXI GAANNNNTTC 2 cut(s) 474, 1263
MscI TGGCCA 1 cut(s) 1062
MseI TTAA 5 cut(s) 78, 336, 642, 1197, 1458
Msp20I TGGCCA 1 cut(s) 1062
MspA1I CMGCKG 1 cut(s) 1502
MspI CCGG 1 cut(s) 165
MspR9I CCNGG 3 cut(s) 166, 1124, 1428
MunI CAATTG 1 cut(s) 204
Mva1269I GAATGC 1 cut(s) 428
MvaI CCWGG 2 cut(s) 1124, 1428
MwoI GCNNNNNNNGC 2 cut(s) 1312, 1519
NciI CCSGG 1 cut(s) 166
NdeI CATATG 1 cut(s) 751
NdeII GATC 7 cut(s) 211, 340, 411, 496, 763, 1068, 1414
NlaIII CATG 6 cut(s) 128, 301, 535, 569, 1283, 1343
NlaIV GGNNCC 1 cut(s) 1042
NmeAIII GCCGAG 1 cut(s) 1250
NmuCI GTSAC 1 cut(s) 178
NsbI TGCGCA 1 cut(s) 302
NsiI ATGCAT 1 cut(s) 1375
NspI RCATGY 3 cut(s) 301, 535, 569
PaeI GCATGC 2 cut(s) 301, 569
PagI TCATGA 1 cut(s) 1279
PctI GAATGC 1 cut(s) 428
PdmI GAANNNNTTC 2 cut(s) 474, 1263
PfeI GAWTC 3 cut(s) 35, 941, 1320
PkrI GCNGC 6 cut(s) 248, 829, 1308, 1465, 1501, 1512
PleI GAGTC 1 cut(s) 922
PpsI GAGTC 1 cut(s) 922
Ppu21I YACGTR 1 cut(s) 308
Psp6I CCWGG 2 cut(s) 1122, 1426
PspFI CCCAGC 2 cut(s) 674, 996
PspGI CCWGG 2 cut(s) 1122, 1426
PspN4I GGNNCC 1 cut(s) 1042
PspPI GGNCC 3 cut(s) 29, 1126, 1133
PstI CTGCAG 1 cut(s) 829
PsuI RGATCY 2 cut(s) 211, 496
PvuII CAGCTG 1 cut(s) 1502
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
SaqAI TTAA 5 cut(s) 78, 336, 642, 1197, 1458
SatI GCNGC 6 cut(s) 247, 828, 1307, 1464, 1500, 1511
Sau3AI GATC 7 cut(s) 211, 340, 411, 496, 763, 1068, 1414
Sau96I GGNCC 3 cut(s) 29, 1126, 1133
SchI GAGTC 1 cut(s) 922
ScrFI CCNGG 3 cut(s) 166, 1124, 1428
SduI GDGCHC 4 cut(s) 583, 854, 1030, 1519
SfaNI GCATC 4 cut(s) 313, 1096, 1160, 1275
SfcI CTRYAG 1 cut(s) 825
SinI GGWCC 2 cut(s) 29, 1133
SmlI CTYRAG 2 cut(s) 65, 232
SmoI CTYRAG 2 cut(s) 65, 232
SphI GCATGC 2 cut(s) 301, 569
SsiI CCGC 4 cut(s) 588, 887, 1510, 1513
SspMI CTAG 3 cut(s) 50, 560, 1055
StyD4I CCNGG 3 cut(s) 164, 1122, 1426
StyI CCWWGG 2 cut(s) 520, 1129
TaaI ACNGT 4 cut(s) 334, 466, 474, 684
TaiI ACGT 3 cut(s) 310, 396, 1426
TatI WGTACW 1 cut(s) 357
TauI GCSGC 1 cut(s) 1513
TfiI GAWTC 3 cut(s) 35, 941, 1320
Tru1I TTAA 5 cut(s) 78, 336, 642, 1197, 1458
Tru9I TTAA 5 cut(s) 78, 336, 642, 1197, 1458
TscAI CASTG 1 cut(s) 189
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 5 cut(s) 246, 827, 1306, 1463, 1499
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 5 cut(s) 17, 99, 108, 768, 1425
TspGWI ACGGA 1 cut(s) 526
TspRI CASTG 1 cut(s) 189
VpaK11BI GGWCC 2 cut(s) 29, 1133
XapI RAATTY 5 cut(s) 113, 839, 1012, 1018, 1171
XceI RCATGY 3 cut(s) 301, 535, 569
XmiI GTMKAC 1 cut(s) 1190
XmnI GAANNNNTTC 2 cut(s) 474, 1263
XspI CTAG 3 cut(s) 50, 560, 1055
Zsp2I ATGCAT 1 cut(s) 1375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.