Rw4G009410

Calmodulin binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0007252
Physical Location & Seq
Reverse (-)
21160568 .. 21168291
7724 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G009410.1

Sequence Viewer

Length: 1929 bp
ATGCAGAGACAGACTAGACTCATGGAGAGGAACAACTCCATGAGAGGGAAGAGGCAAATGGAAGGGGAAGAGGAGGAGCAGCCGGAGCGGAAGCGGCCGGCTCTCGCTAGTGTAATTGTAGAAGCTCTGAAGGTGGACAGTTTGCAGAAGCTATGCTCGTCACTGGAGCCTATTCTTCGAAGAGTTGTCAGTGAAGAAGTGGAACGTGCATTGGCCAAGTTGGGCCCTCCAAGACTAAATGGAAGGTCTTCCCCCAAACGGATTGAAGGTTCAAATGATCGGAACTTGCAACTGCAGTTTCAGTCCAATCTGGCTCTCCCCATTTTCACAGGAGGGAAAGTTGAAGGCGAGCAGGGGGCTTCAATTCATGTTGTTTTATTTGATATGAACACTCAGCGTGTTGTAACATCTGGACCTGAATCCTCTATAAAACTAGATATTGTTGTGCTTGAGGGTGATTTTAACAATGAAGATGATGAAGGCTGGACTGAAGAAGAATTTGAAAGCCATGTGGTCAAAGAGCGTGAAGGAAAGAGACCTCTTTTGACCGGGGAATTGCAAGTGACACTGAAGGAAGGGGTAGGGACAATTGGGGATCTGACTTTTACAGATAACTCAAGTTGGATACGCAGCAGGAAGTTTAGGCTTGGATTGAAAGTTGCTTCAGGATTTTGTGAGGGCATGCGCATACGTGAAGCGAAAACAGATGCTTTTACTGTTAAAGATCACAGAGGGGAATTGTACAAGAAACATTACCCACCAGCACTAAATGATGACGTATGGAGACTGGAGAAGATCGGAAAGGATGGGGCATTCCATAAGAGGCTAAACAATTCAGGAATACACACAGTTGAACAGTTTCTTCAGCTTGTGGTCAAAGATCCTCAAAAATTACGGAGTATCCTTGGAAGTGGCATGTCAAATAAGATGTGGGAAGCTCTCCTAGAGCATGCAAAGACTTGTGCCCTTAGCGGAAAGCTTTATGTTTATTATCCTGAAGATACAAGAAATGTTGGTGTTGCTTTTAACAACATCTATGAGCTGAGTGGCTTGATTGCTGGGGAACAGTATCATTCTGCCGATACTCTTTCCGACAGCCAGAAGCTCTATGTAGATACGCTTGTGAAGAAAGCATATGAAAATTGGGATCAGGTTGTTGAGTATGATGGTAAATCACTTCTGAACACCAAACAAAATAAGAGGTCAACTGCAGCCCGAAACGAATTTCAAATGGGCTCAATCAGTTACTCCGGTTCTTCAGACCAGCATCCGCAACTACCTCGCCTGACAAATCCAGTTCCCTCAGAACAGACTCTTCTATCTGATTCAGCCCTACCGATTGGAGGGTATAATGATAATCTGTCAACAAGGTATATGACCCAGCAACAAATTGTGAATTCAAATTCCCGTGCCCAGTTTGAAGGCACCTCATTTGCACTAGATGGCCAGTTGATCAACAGCTCTCATCAGCCTCAAAGCATAACAAATGATGCCAATGCTGTTGGCCTGGCCCTTGGTCCTCCACAATCATCGACATCAGGATTTCAGGCTATAAATTCTTCTATTCAGCCGTCTACTCTTAATCCTTTAGATGACTGGGGTACAAGCCGAGAAAAAGGAGTTGATGACTTCTTCTCAGAAGAAGATATTCGCATTAGAAGTCATGAGATGCTTGAAAATGAGGATATGCAGCACTTGCTGAGAATCTTCAGTATGGGAGGCCATGCCTCTATTGATATGCCTGATGATGGATATGCATACCCATATATGCCATCTCCAATGCCCAACTTTGATGAAGATCGCACACGTCCTGGCAAAGCTGTTGTTGGGTGGCTGAAAATTAAGGCAGCTATGAGATGGGGTTTCTTTGTCAGAAAGAAAGCAGCTGAAAGGCGGCGGGCACAGCTTGTGGAGTTAGATGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

642

Amino Acids

72.18

Weight (kDa)

5.78

Isoelectric Point (pI)

48.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 96 - 243 1.2e-64 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 255 - 321 7.9e-34 Calmodulin binding protein central domain
Calmod_bind_C PF20452 326 - 388 2.8e-25 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 686
AccB1I GGYRCC 1 cut(s) 1424
AccBSI CCGCTC 1 cut(s) 88
AccI GTMKAC 1 cut(s) 1574
AciI CCGC 6 cut(s) 88, 94, 972, 1271, 1894, 1897
AclWI GGATC 3 cut(s) 603, 875, 1155
AcoI YGGCCR 3 cut(s) 95, 213, 1444
AcsI RAATTY 5 cut(s) 497, 1223, 1396, 1402, 1555
AcuI CTGAAG 8 cut(s) 149, 510, 590, 648, 848, 1017, 1242, 1693
AfaI GTAC 2 cut(s) 743, 1603
AfiI CCNNNNNNNGG 4 cut(s) 45, 258, 1343, 1748
AflIII ACRYGT 1 cut(s) 1805
AjiI CACGTC 1 cut(s) 1808
AjnI CCWGG 2 cut(s) 1506, 1810
AloI GAACNNNNNNTCC 2 cut(s) 253, 285
Alw26I GTCTC 2 cut(s) 529, 778
AlwI GGATC 3 cut(s) 603, 875, 1155
AoxI GGCC 7 cut(s) 95, 213, 223, 1444, 1504, 1509, 1720
ApaI GGGCCC 1 cut(s) 227
ApeKI GCWGC 6 cut(s) 79, 630, 1211, 1690, 1847, 1883
ApoI RAATTY 5 cut(s) 497, 1223, 1396, 1402, 1555
Asp700I GAANNNNTTC 3 cut(s) 247, 858, 1647
AspLEI GCGC 1 cut(s) 687
AspS9I GGNCC 5 cut(s) 223, 224, 413, 1510, 1517
AsuC2I CCSGG 1 cut(s) 550
AsuHPI GGTGA 1 cut(s) 467
AsuII TTCGAA 1 cut(s) 178
AvaII GGWCC 2 cut(s) 413, 1517
BaeGI GKGCMC 4 cut(s) 227, 967, 1414, 1903
BaeI ACNNNNGTAYC 2 cut(s) 1107, 1140
BalI TGGCCA 2 cut(s) 215, 1446
BanI GGYRCC 1 cut(s) 1424
BanII GRGCYC 2 cut(s) 227, 1238
BbsI GAAGAC 1 cut(s) 240
BbvI GCAGC 6 cut(s) 91, 642, 1223, 1702, 1859, 1895
BccI CCATC 6 cut(s) 800, 1160, 1436, 1742, 1780, 1851
BceAI ACGGC 1 cut(s) 1555
BcgI CGANNNNNNTGC 2 cut(s) 1262, 1296
BciT130I CCWGG 2 cut(s) 1508, 1812
BciVI GTATCC 2 cut(s) 618, 911
BclI TGATCA 1 cut(s) 1452
BcnI CCSGG 1 cut(s) 550
BcoDI GTCTC 2 cut(s) 529, 778
BfaI CTAG 5 cut(s) 15, 108, 434, 944, 1439
BfmI CTRYAG 2 cut(s) 293, 1209
BfuI GTATCC 2 cut(s) 618, 911
BisI GCNGC 8 cut(s) 80, 95, 631, 1212, 1691, 1848, 1884, 1895
BlsI GCNGC 8 cut(s) 81, 96, 632, 1213, 1692, 1849, 1885, 1896
Bme1390I CCNGG 3 cut(s) 550, 1508, 1812
Bme18I GGWCC 2 cut(s) 413, 1517
BmgBI CACGTC 1 cut(s) 1808
BmgT120I GGNCC 5 cut(s) 223, 224, 413, 1510, 1517
BmiI GGNNCC 3 cut(s) 168, 225, 1426
BmrFI CCNGG 3 cut(s) 550, 1508, 1812
BmrI ACTGGG 2 cut(s) 1408, 1606
BmsI GCATC 4 cut(s) 697, 1276, 1480, 1659
BmuI ACTGGG 2 cut(s) 1408, 1606
BpiI GAAGAC 1 cut(s) 240
BpmI CTGGAG 2 cut(s) 185, 809
Bpu10I CCTNAGC 1 cut(s) 968
Bpu14I TTCGAA 1 cut(s) 178
BpuEI CTTGAG 2 cut(s) 470, 601
BpuMI CCSGG 1 cut(s) 550
BsaAI YACGTR 1 cut(s) 692
BsaBI GATNNNNATC 1 cut(s) 1797
BsaI GGTCTC 1 cut(s) 529
BsaJI CCNNGG 3 cut(s) 549, 904, 1513
BsaWI WCCGGW 1 cut(s) 1250
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bsc4I CCNNNNNNNGG 4 cut(s) 45, 258, 1343, 1748
Bse118I RCCGGY 1 cut(s) 97
Bse1I ACTGG 6 cut(s) 168, 792, 1295, 1414, 1447, 1601
Bse8I GATNNNNATC 1 cut(s) 1797
BseBI CCWGG 2 cut(s) 1508, 1812
BseDI CCNNGG 3 cut(s) 549, 904, 1513
BseGI GGATG 2 cut(s) 811, 1267
BseJI GATNNNNATC 1 cut(s) 1797
BseLI CCNNNNNNNGG 4 cut(s) 45, 258, 1343, 1748
BseMII CTCAG 5 cut(s) 407, 1034, 1317, 1650, 1691
BseNI ACTGG 6 cut(s) 168, 792, 1295, 1414, 1447, 1601
BseRI GAGGAG 2 cut(s) 86, 89
BseSI GKGCMC 4 cut(s) 227, 967, 1414, 1903
BseX3I CGGCCG 1 cut(s) 95
BseXI GCAGC 6 cut(s) 91, 642, 1223, 1702, 1859, 1895
BseYI CCCAGC 2 cut(s) 1058, 1380
Bsh1285I CGRYCG 1 cut(s) 98
BshFI GGCC 7 cut(s) 97, 215, 225, 1446, 1506, 1511, 1722
BshNI GGYRCC 1 cut(s) 1424
BsiEI CGRYCG 1 cut(s) 98
BsiSI CCGG 4 cut(s) 83, 98, 549, 1251
BslFI GGGAC 1 cut(s) 598
BslI CCNNNNNNNGG 4 cut(s) 45, 258, 1343, 1748
BsmAI GTCTC 2 cut(s) 529, 778
BsmFI GGGAC 1 cut(s) 598
BsmI GAATGC 1 cut(s) 812
BsnI GGCC 7 cut(s) 97, 215, 225, 1446, 1506, 1511, 1722
Bso31I GGTCTC 1 cut(s) 529
Bsp119I TTCGAA 1 cut(s) 178
Bsp120I GGGCCC 1 cut(s) 223
Bsp1286I GDGCHC 5 cut(s) 227, 967, 1238, 1414, 1903
Bsp1407I TGTACA 1 cut(s) 741
Bsp143I GATC 8 cut(s) 277, 595, 724, 795, 880, 1147, 1452, 1798
BspACI CCGC 6 cut(s) 88, 94, 972, 1271, 1894, 1897
BspANI GGCC 7 cut(s) 97, 215, 225, 1446, 1506, 1511, 1722
BspCNI CTCAG 5 cut(s) 406, 1035, 1316, 1649, 1692
BspHI TCATGA 1 cut(s) 1663
BspLI GGNNCC 3 cut(s) 168, 225, 1426
BspMAI CTGCAG 2 cut(s) 297, 1213
BspPI GGATC 3 cut(s) 603, 875, 1155
BspT104I TTCGAA 1 cut(s) 178
BspT107I GGYRCC 1 cut(s) 1424
BspTNI GGTCTC 1 cut(s) 529
BsrBI CCGCTC 1 cut(s) 88
BsrFI RCCGGY 1 cut(s) 97
BsrGI TGTACA 1 cut(s) 741
BsrI ACTGG 6 cut(s) 168, 792, 1295, 1414, 1447, 1601
BssAI RCCGGY 1 cut(s) 97
BssECI CCNNGG 3 cut(s) 549, 904, 1513
BssMI GATC 8 cut(s) 277, 595, 724, 795, 880, 1147, 1452, 1798
BssT1I CCWWGG 2 cut(s) 904, 1513
Bst2UI CCWGG 2 cut(s) 1508, 1812
Bst4CI ACNGT 5 cut(s) 140, 718, 850, 858, 1068
Bst6I CTCTTC 4 cut(s) 44, 63, 175, 1320
BstAPI GCANNNNNTGC 1 cut(s) 1696
BstAUI TGTACA 1 cut(s) 741
BstBAI YACGTR 1 cut(s) 692
BstBI TTCGAA 1 cut(s) 178
BstC8I GCNNGC 5 cut(s) 99, 350, 683, 951, 1899
BstDEI CTNAG 6 cut(s) 393, 968, 1043, 1303, 1636, 1700
BstF5I GGATG 2 cut(s) 811, 1267
BstHHI GCGC 1 cut(s) 687
BstKTI GATC 8 cut(s) 280, 598, 727, 798, 883, 1150, 1455, 1801
BstMAI GTCTC 2 cut(s) 529, 778
BstMBI GATC 8 cut(s) 277, 595, 724, 795, 880, 1147, 1452, 1798
BstMCI CGRYCG 1 cut(s) 98
BstMWI GCNNNNNNNGC 4 cut(s) 85, 94, 1696, 1903
BstNI CCWGG 2 cut(s) 1508, 1812
BstNSI RCATGY 3 cut(s) 685, 919, 953
BstSCI CCNGG 3 cut(s) 548, 1506, 1810
BstSFI CTRYAG 2 cut(s) 293, 1209
BstSLI GKGCMC 4 cut(s) 227, 967, 1414, 1903
BstV1I GCAGC 6 cut(s) 91, 642, 1223, 1702, 1859, 1895
BstV2I GAAGAC 1 cut(s) 240
BstX2I RGATCY 2 cut(s) 595, 880
BstYI RGATCY 2 cut(s) 595, 880
BstZI CGGCCG 1 cut(s) 95
BsuI GTATCC 2 cut(s) 618, 911
BsuRI GGCC 7 cut(s) 97, 215, 225, 1446, 1506, 1511, 1722
BtrI CACGTC 1 cut(s) 1808
BtsCI GGATG 2 cut(s) 811, 1267
BtsIMutI CAGTG 3 cut(s) 161, 196, 566
Cac8I GCNNGC 5 cut(s) 99, 350, 683, 951, 1899
CciI TCATGA 1 cut(s) 1663
CfoI GCGC 1 cut(s) 687
Cfr10I RCCGGY 1 cut(s) 97
Cfr13I GGNCC 5 cut(s) 223, 224, 413, 1510, 1517
Csp6I GTAC 2 cut(s) 742, 1602
CviAII CATG 9 cut(s) 22, 40, 368, 509, 682, 916, 950, 1664, 1724
CviQI GTAC 2 cut(s) 742, 1602
DdeI CTNAG 6 cut(s) 393, 968, 1043, 1303, 1636, 1700
DpnI GATC 8 cut(s) 279, 597, 726, 797, 882, 1149, 1454, 1800
DpnII GATC 8 cut(s) 277, 595, 724, 795, 880, 1147, 1452, 1798
EaeI YGGCCR 3 cut(s) 95, 213, 1444
EagI CGGCCG 1 cut(s) 95
Eam1104I CTCTTC 4 cut(s) 44, 63, 175, 1320
EarI CTCTTC 4 cut(s) 44, 63, 175, 1320
EclXI CGGCCG 1 cut(s) 95
Eco130I CCWWGG 2 cut(s) 904, 1513
Eco24I GRGCYC 2 cut(s) 227, 1238
Eco31I GGTCTC 1 cut(s) 529
Eco47I GGWCC 2 cut(s) 413, 1517
Eco52I CGGCCG 1 cut(s) 95
Eco57I CTGAAG 8 cut(s) 149, 510, 590, 648, 848, 1017, 1242, 1693
EcoO109I RGGNCCY 1 cut(s) 224
EcoRI GAATTC 1 cut(s) 1396
EcoRII CCWGG 2 cut(s) 1506, 1810
EcoT14I CCWWGG 2 cut(s) 904, 1513
EcoT22I ATGCAT 1 cut(s) 1759
EcoT38I GRGCYC 2 cut(s) 227, 1238
ErhI CCWWGG 2 cut(s) 904, 1513
FaeI CATG 9 cut(s) 25, 43, 371, 512, 685, 919, 953, 1667, 1727
FaqI GGGAC 1 cut(s) 598
FatI CATG 9 cut(s) 21, 39, 367, 508, 681, 915, 949, 1663, 1723
FauI CCCGC 1 cut(s) 1890
FauNDI CATATG 1 cut(s) 1135
FbaI TGATCA 1 cut(s) 1452
FblI GTMKAC 1 cut(s) 1574
Fnu4HI GCNGC 8 cut(s) 80, 95, 631, 1212, 1691, 1848, 1884, 1895
FokI GGATG 2 cut(s) 818, 1254
FriOI GRGCYC 2 cut(s) 227, 1238
Fsp4HI GCNGC 8 cut(s) 80, 95, 631, 1212, 1691, 1848, 1884, 1895
FspAI RTGCGCAY 1 cut(s) 686
FspBI CTAG 5 cut(s) 15, 108, 434, 944, 1439
FspI TGCGCA 1 cut(s) 686
GlaI GCGC 1 cut(s) 686
GluI GCNGC 8 cut(s) 80, 95, 631, 1212, 1691, 1848, 1884, 1895
GsaI CCCAGC 2 cut(s) 1062, 1384
GsuI CTGGAG 2 cut(s) 185, 809
HaeIII GGCC 7 cut(s) 97, 215, 225, 1446, 1506, 1511, 1722
HapII CCGG 4 cut(s) 83, 98, 549, 1251
HhaI GCGC 1 cut(s) 687
Hin1II CATG 9 cut(s) 25, 43, 371, 512, 685, 919, 953, 1667, 1727
Hin6I GCGC 1 cut(s) 685
HinP1I GCGC 1 cut(s) 685
HincII GTYRAC 2 cut(s) 1206, 1365
HindII GTYRAC 2 cut(s) 1206, 1365
HindIII AAGCTT 1 cut(s) 977
HinfI GANTC 5 cut(s) 18, 419, 1312, 1325, 1704
HpaII CCGG 4 cut(s) 83, 98, 549, 1251
HphI GGTGA 1 cut(s) 467
Hpy166II GTNNAC 4 cut(s) 136, 1206, 1365, 1575
Hpy188III TCNNGA 6 cut(s) 411, 666, 837, 995, 1539, 1664
Hpy8I GTNNAC 4 cut(s) 136, 1206, 1365, 1575
HpyCH4III ACNGT 5 cut(s) 140, 718, 850, 858, 1068
HpyCH4IV ACGT 4 cut(s) 205, 691, 777, 1807
HpyF10VI GCNNNNNNNGC 4 cut(s) 85, 94, 1696, 1903
HpyF3I CTNAG 6 cut(s) 393, 968, 1043, 1303, 1636, 1700
HpySE526I ACGT 4 cut(s) 205, 691, 777, 1807
Hsp92II CATG 9 cut(s) 25, 43, 371, 512, 685, 919, 953, 1667, 1727
HspAI GCGC 1 cut(s) 685
KroI GCCGGC 1 cut(s) 97
KroNI GCCGGC 1 cut(s) 99
Ksp22I TGATCA 1 cut(s) 1452
Kzo9I GATC 8 cut(s) 277, 595, 724, 795, 880, 1147, 1452, 1798
LmnI GCTCC 3 cut(s) 76, 85, 166
Lsp1109I GCAGC 6 cut(s) 91, 642, 1223, 1702, 1859, 1895
LweI GCATC 4 cut(s) 697, 1276, 1480, 1659
MaeI CTAG 5 cut(s) 15, 108, 434, 944, 1439
MaeII ACGT 4 cut(s) 205, 691, 777, 1807
MaeIII GTNAC 4 cut(s) 159, 403, 562, 1244
MalI GATC 8 cut(s) 279, 597, 726, 797, 882, 1149, 1454, 1800
MbiI CCGCTC 1 cut(s) 88
MboI GATC 8 cut(s) 277, 595, 724, 795, 880, 1147, 1452, 1798
MfeI CAATTG 1 cut(s) 588
MflI RGATCY 2 cut(s) 595, 880
MhlI GDGCHC 5 cut(s) 227, 967, 1238, 1414, 1903
MlsI TGGCCA 2 cut(s) 215, 1446
MluNI TGGCCA 2 cut(s) 215, 1446
MlyI GAGTC 2 cut(s) 12, 1306
MmeI TCCRAC 2 cut(s) 602, 1116
Mox20I TGGCCA 2 cut(s) 215, 1446
Mph1103I ATGCAT 1 cut(s) 1759
MroNI GCCGGC 1 cut(s) 97
MroXI GAANNNNTTC 3 cut(s) 247, 858, 1647
MscI TGGCCA 2 cut(s) 215, 1446
MseI TTAA 5 cut(s) 462, 720, 1026, 1581, 1842
Msp20I TGGCCA 2 cut(s) 215, 1446
MspA1I CMGCKG 1 cut(s) 1886
MspI CCGG 4 cut(s) 83, 98, 549, 1251
MspR9I CCNGG 3 cut(s) 550, 1508, 1812
MunI CAATTG 1 cut(s) 588
Mva1269I GAATGC 1 cut(s) 812
MvaI CCWGG 2 cut(s) 1508, 1812
MwoI GCNNNNNNNGC 4 cut(s) 85, 94, 1696, 1903
NaeI GCCGGC 1 cut(s) 99
NciI CCSGG 1 cut(s) 550
NdeI CATATG 1 cut(s) 1135
NdeII GATC 8 cut(s) 277, 595, 724, 795, 880, 1147, 1452, 1798
NgoMIV GCCGGC 1 cut(s) 97
NlaIII CATG 9 cut(s) 25, 43, 371, 512, 685, 919, 953, 1667, 1727
NlaIV GGNNCC 3 cut(s) 168, 225, 1426
NmeAIII GCCGAG 1 cut(s) 1634
NmuCI GTSAC 2 cut(s) 159, 562
NsbI TGCGCA 1 cut(s) 686
NsiI ATGCAT 1 cut(s) 1759
NspI RCATGY 3 cut(s) 685, 919, 953
NspV TTCGAA 1 cut(s) 178
PaeI GCATGC 2 cut(s) 685, 953
PagI TCATGA 1 cut(s) 1663
PctI GAATGC 1 cut(s) 812
PdiI GCCGGC 1 cut(s) 99
PdmI GAANNNNTTC 3 cut(s) 247, 858, 1647
PfeI GAWTC 3 cut(s) 419, 1325, 1704
PkrI GCNGC 8 cut(s) 81, 96, 632, 1213, 1692, 1849, 1885, 1896
PleI GAGTC 2 cut(s) 12, 1306
PpsI GAGTC 2 cut(s) 12, 1306
Ppu21I YACGTR 1 cut(s) 692
Psp6I CCWGG 2 cut(s) 1506, 1810
PspFI CCCAGC 2 cut(s) 1058, 1380
PspGI CCWGG 2 cut(s) 1506, 1810
PspN4I GGNNCC 3 cut(s) 168, 225, 1426
PspOMI GGGCCC 1 cut(s) 223
PspPI GGNCC 5 cut(s) 223, 224, 413, 1510, 1517
PstI CTGCAG 2 cut(s) 297, 1213
PsuI RGATCY 2 cut(s) 595, 880
PvuII CAGCTG 1 cut(s) 1886
RsaI GTAC 2 cut(s) 743, 1603
RsaNI GTAC 2 cut(s) 742, 1602
SaqAI TTAA 5 cut(s) 462, 720, 1026, 1581, 1842
SatI GCNGC 8 cut(s) 80, 95, 631, 1212, 1691, 1848, 1884, 1895
Sau3AI GATC 8 cut(s) 277, 595, 724, 795, 880, 1147, 1452, 1798
Sau96I GGNCC 5 cut(s) 223, 224, 413, 1510, 1517
SchI GAGTC 2 cut(s) 12, 1306
ScrFI CCNGG 3 cut(s) 550, 1508, 1812
SduI GDGCHC 5 cut(s) 227, 967, 1238, 1414, 1903
SfaNI GCATC 4 cut(s) 697, 1276, 1480, 1659
SfcI CTRYAG 2 cut(s) 293, 1209
SfuI TTCGAA 1 cut(s) 178
SinI GGWCC 2 cut(s) 413, 1517
SmlI CTYRAG 2 cut(s) 449, 616
SmoI CTYRAG 2 cut(s) 449, 616
SphI GCATGC 2 cut(s) 685, 953
SsiI CCGC 6 cut(s) 88, 94, 972, 1271, 1894, 1897
SspMI CTAG 5 cut(s) 15, 108, 434, 944, 1439
StyD4I CCNGG 3 cut(s) 548, 1506, 1810
StyI CCWWGG 2 cut(s) 904, 1513
TaaI ACNGT 5 cut(s) 140, 718, 850, 858, 1068
TaiI ACGT 4 cut(s) 208, 694, 780, 1810
TaqI TCGA 2 cut(s) 178, 1532
TatI WGTACW 1 cut(s) 741
TauI GCSGC 2 cut(s) 97, 1897
TfiI GAWTC 3 cut(s) 419, 1325, 1704
Tru1I TTAA 5 cut(s) 462, 720, 1026, 1581, 1842
Tru9I TTAA 5 cut(s) 462, 720, 1026, 1581, 1842
TscAI CASTG 3 cut(s) 168, 196, 573
TseFI GTSAC 2 cut(s) 159, 562
TseI GCWGC 6 cut(s) 79, 630, 1211, 1690, 1847, 1883
Tsp45I GTSAC 2 cut(s) 159, 562
TspDTI ATGAA 6 cut(s) 356, 401, 483, 492, 1152, 1809
TspGWI ACGGA 2 cut(s) 274, 910
TspRI CASTG 3 cut(s) 168, 196, 573
VpaK11BI GGWCC 2 cut(s) 413, 1517
XapI RAATTY 5 cut(s) 497, 1223, 1396, 1402, 1555
XceI RCATGY 3 cut(s) 685, 919, 953
XmiI GTMKAC 1 cut(s) 1574
XmnI GAANNNNTTC 3 cut(s) 247, 858, 1647
XspI CTAG 5 cut(s) 15, 108, 434, 944, 1439
Zsp2I ATGCAT 1 cut(s) 1759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.