MD16G1271200.v1.1

Heat shock factor protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012798
Physical Location & Seq
Reverse (-)
35258373 .. 35264471
6099 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1271200.v1.1.491

Sequence Viewer

Length: 1551 bp
ATGGAGGGTGCTAATAACAACAACGGCGACGAGATACCAGCGGCCGGCGGCGCACACCAGGCGGATTCGGGTTTAAGTTTGGCTCCGGCGCCGGCGCCCTTACTGAACTCCAACGCCCCGCCGCCGTTCCTGAGCAAGACGTACGACATGGTGGACGACCCGGCTACCGACCCGGTCGTGTCCTGGAGCCCCACCAACAACAGCTTCGTGGTTTGGAACCCGCCGGAGTTCGCTAGGGACCTCCTGCCCAAGTACTTCAAGCACAATAACTTCTCTAGCTTTGTTAGGCAGTTGAACACCTACGGATTTAGGAAGATTGATCCAGACCGCTGGGAATTTGCGAATGAGGGATTTTTGAGGGGTAAAAAACATCTCCTTAAGAGCATCAATCGGCGTAAACCTACCCATGGACATAGTCATCAACAGCAACAGCCATCACATGGACAGAATTCAATGGGTGCGTGTGTAGAAGTTGGGAAGTTTGGCCTCGAGGAAGAGGTTGAGAGGCTAAAAAGGGACAAGAATGTGCTTATGCAGGAACTTATCAGGTTGAGGCAGCAGCAGCAGTCTACTGATAACCAGCTACAAACAATGGTGCAGCGTCTTCAGGGCATGGAGCAGCGGCAACAACAGATGATGTCATTCCTTGCAAAGGCAATGCATAGCCCTGATTTCTTCACTCAATTTGTACAGAAAAATGAGAGCAATAGGCGCATAACTGAAGTCAACAAAAAACGGAGACTTAAGCGGGAAGGTGTTGCTGAGAGAGAGTGTTCTAATGCTCCTGATGGACAGATTGTTAAGTATCAGCCTCCCATGAATGAAGCAGCAAAGGCAATGCTCAGGCAGATCATGAATACAGATACATCATCTCGGTTGGAATCTTTTAATAACAATTCTGATAACTTCTTGATTGGTGATGGTTCATCATCATCCAGTGCAATAGACAGTGGGAGTTCTTCAAGCTGCGTGTCAGGAGTGACTCTTCAGGAGGTCCCGCTAACTTCAGTGCATGGCTTGCCTTCTGCAATTTCTGAAACACAATCTCCTCTGCGGGCTGCAAACCCTGGAACAGTTATGAGATCTCCATTCTCAGATGTAAATGCCCTTGTTGGAGAAGAGTCCCCATCAATTCCCATACCTCAGACAAATGTAAATATTCCGGAGCTTTCTCAAATAGCAGATATGTCATCCGAAGGCTTAGTGGATATTCCTGAAGAAAGCATGGCAGCTGAAGACATTGGTGTTGGATTTATTGAAAACATGGCATCTGAAGCAGGTGATGGATTTATGGACCCCACATCATTGGTAATGAATGGGTCATTGCCAATAGATTTTGATGATATTTCGTCGGACATAGAAGCATTTCTAAAGGATTGGGATGATGTTTTACAAAACCCGGGGGCAGATGAAATGGATTCTACTTGTGCAGGAATGCCCATGGAAAATGAAAAGCAGCAGCCAACTGAGAATGGATGGGACAAAACCCAGAATATGGATAATCTGACTGAAAAAATGGGACTTCTTACATCAGAGACCAGAGGGGTGTGA

Protein Analysis

517

Amino Acids

56.94

Weight (kDa)

4.8

Isoelectric Point (pI)

58.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 43 - 132 3.5e-30 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012798)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32330 AT1G32330
fragaria_vesca FvH4_5g22720
malus_domestica MD06G1037900.v1.1 MD16G1271200.v1.1
prunus_persica Prupe.5G031100_v2.0.a1
pyrus_communis pycom16g24070
rosa_chinensis RchiOBHm_Chr7g0211291
rosa_laevigata RLG00000003012
rosa_multiflora Rmu_co8434101.1_g000001 Rmu_sc0001226.1_g000001
rosa_roxburghii Rroxscaffold_3G00247950
rosa_rugosa Rorug07G0124900
rosa_samantha Rh7AG257200 Rh7BG251300 Rh7CG274000 Rh7DG264600
rosa_wichuraiana Rw7G021980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1268
AbsI CCTCGAGG 1 cut(s) 488
Acc36I ACCTGC 1 cut(s) 1268
AccB1I GGYRCC 2 cut(s) 88, 94
AccB7I CCANNNNNTGG 2 cut(s) 440, 1495
AccI GTMKAC 1 cut(s) 569
AccIII TCCGGA 1 cut(s) 1162
AclWI GGATC 1 cut(s) 314
AcoI YGGCCR 1 cut(s) 42
AcsI RAATTY 2 cut(s) 335, 448
AcuI CTGAAG 7 cut(s) 590, 741, 971, 990, 1236, 1254, 1293
AcyI GRCGYC 2 cut(s) 89, 95
AfaI GTAC 3 cut(s) 143, 254, 690
AfiI CCNNNNNNNGG 5 cut(s) 44, 407, 440, 652, 1495
AflII CTTAAG 2 cut(s) 377, 743
AgsI TTSAA 5 cut(s) 259, 295, 453, 963, 1259
AjnI CCWGG 3 cut(s) 57, 182, 1066
AjuI GAANNNNNNNTTGG 2 cut(s) 188, 220
AluBI AGCT 6 cut(s) 204, 279, 583, 966, 1168, 1232
AluI AGCT 6 cut(s) 204, 279, 583, 966, 1168, 1232
Alw26I GTCTC 2 cut(s) 733, 1529
AlwI GGATC 1 cut(s) 314
Ama87I CYCGRG 2 cut(s) 488, 1399
Aor13HI TCCGGA 1 cut(s) 1162
AoxI GGCC 2 cut(s) 42, 484
ApoI RAATTY 2 cut(s) 335, 448
Asp700I GAANNNNTTC 1 cut(s) 1365
AspLEI GCGC 4 cut(s) 53, 91, 97, 714
AspS9I GGNCC 3 cut(s) 238, 994, 1294
AsuC2I CCSGG 4 cut(s) 161, 173, 1400, 1401
AsuHPI GGTGA 2 cut(s) 929, 1292
AvaI CYCGRG 2 cut(s) 488, 1399
AvaII GGWCC 3 cut(s) 238, 994, 1294
BanI GGYRCC 2 cut(s) 88, 94
BanII GRGCYC 1 cut(s) 191
BbsI GAAGAC 2 cut(s) 596, 1242
BccI CCATC 6 cut(s) 442, 782, 914, 1135, 1277, 1470
BceAI ACGGC 2 cut(s) 40, 109
BciT130I CCWGG 3 cut(s) 59, 184, 1068
BcnI CCSGG 4 cut(s) 161, 173, 1400, 1401
BcoDI GTCTC 2 cut(s) 733, 1529
BfaI CTAG 2 cut(s) 234, 276
BfoI RGCGCY 2 cut(s) 92, 98
BfrI CTTAAG 2 cut(s) 377, 743
BfuAI ACCTGC 1 cut(s) 1268
BglII AGATCT 1 cut(s) 1082
BmcAI AGTACT 1 cut(s) 254
Bme1390I CCNGG 7 cut(s) 59, 161, 173, 184, 1068, 1400, 1401
Bme18I GGWCC 3 cut(s) 238, 994, 1294
BmeT110I CYCGRG 2 cut(s) 488, 1399
BmgT120I GGNCC 3 cut(s) 238, 994, 1294
BmiI GGNNCC 8 cut(s) 84, 90, 96, 188, 218, 239, 996, 1296
BmrFI CCNGG 7 cut(s) 59, 161, 173, 184, 1068, 1400, 1401
BmsI GCATC 2 cut(s) 393, 1277
BpiI GAAGAC 2 cut(s) 596, 1242
BpmI CTGGAG 1 cut(s) 205
Bpu10I CCTNAGC 2 cut(s) 131, 842
BpuMI CCSGG 4 cut(s) 161, 173, 1400, 1401
BsaHI GRCGYC 2 cut(s) 89, 95
BsaI GGTCTC 1 cut(s) 1529
BsaJI CCNNGG 5 cut(s) 406, 1066, 1399, 1400, 1440
BsaWI WCCGGW 1 cut(s) 1162
BsaXI ACNNNNNCTCC 2 cut(s) 1030, 1060
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 407, 440, 652, 1495
Bse118I RCCGGY 2 cut(s) 44, 91
Bse1I ACTGG 1 cut(s) 936
Bse3DI GCAATG 3 cut(s) 663, 843, 1322
BseAI TCCGGA 1 cut(s) 1162
BseBI CCWGG 3 cut(s) 59, 184, 1068
BseDI CCNNGG 5 cut(s) 406, 1066, 1399, 1400, 1440
BseGI GGATG 4 cut(s) 932, 1190, 1387, 1481
BseLI CCNNNNNNNGG 5 cut(s) 44, 407, 440, 652, 1495
BseMI GCAATG 3 cut(s) 663, 843, 1322
BseMII CTCAG 6 cut(s) 122, 753, 856, 1107, 1157, 1458
BseNI ACTGG 1 cut(s) 936
BseRI GAGGAG 1 cut(s) 1038
BseX3I CGGCCG 1 cut(s) 42
BseYI CCCAGC 1 cut(s) 330
BsgI GTGCAG 2 cut(s) 617, 1449
Bsh1285I CGRYCG 2 cut(s) 45, 177
BshFI GGCC 2 cut(s) 44, 486
BshNI GGYRCC 2 cut(s) 88, 94
BsiEI CGRYCG 2 cut(s) 45, 177
BsiHKCI CYCGRG 2 cut(s) 488, 1399
BsiSI CCGG 8 cut(s) 45, 86, 92, 161, 173, 224, 1163, 1400
BsiWI CGTACG 1 cut(s) 141
BslFI GGGAC 6 cut(s) 251, 530, 980, 1108, 1493, 1533
BslI CCNNNNNNNGG 5 cut(s) 44, 407, 440, 652, 1495
BsmAI GTCTC 2 cut(s) 733, 1529
BsmFI GGGAC 6 cut(s) 251, 530, 980, 1108, 1493, 1533
BsmI GAATGC 1 cut(s) 1440
BsnI GGCC 2 cut(s) 44, 486
Bso31I GGTCTC 1 cut(s) 1529
BsoBI CYCGRG 2 cut(s) 488, 1399
Bsp1286I GDGCHC 1 cut(s) 191
Bsp13I TCCGGA 1 cut(s) 1162
Bsp1407I TGTACA 1 cut(s) 688
Bsp143I GATC 3 cut(s) 319, 849, 1082
Bsp19I CCATGG 2 cut(s) 406, 1440
BspANI GGCC 2 cut(s) 44, 486
BspCNI CTCAG 6 cut(s) 123, 754, 855, 1106, 1156, 1459
BspEI TCCGGA 1 cut(s) 1162
BspHI TCATGA 1 cut(s) 852
BspLI GGNNCC 8 cut(s) 84, 90, 96, 188, 218, 239, 996, 1296
BspMI ACCTGC 1 cut(s) 1268
BspPI GGATC 1 cut(s) 314
BspT107I GGYRCC 2 cut(s) 88, 94
BspTI CTTAAG 2 cut(s) 377, 743
BspTNI GGTCTC 1 cut(s) 1529
BsrDI GCAATG 3 cut(s) 663, 843, 1322
BsrFI RCCGGY 2 cut(s) 44, 91
BsrGI TGTACA 1 cut(s) 688
BsrI ACTGG 1 cut(s) 936
BssAI RCCGGY 2 cut(s) 44, 91
BssECI CCNNGG 5 cut(s) 406, 1066, 1399, 1400, 1440
BssMI GATC 3 cut(s) 319, 849, 1082
BssNI GRCGYC 2 cut(s) 89, 95
BssT1I CCWWGG 2 cut(s) 406, 1440
Bst2UI CCWGG 3 cut(s) 59, 184, 1068
Bst4CI ACNGT 2 cut(s) 950, 1075
Bst6I CTCTTC 3 cut(s) 489, 990, 1113
BstACI GRCGYC 2 cut(s) 89, 95
BstAFI CTTAAG 2 cut(s) 377, 743
BstAPI GCANNNNNTGC 1 cut(s) 1018
BstAUI TGTACA 1 cut(s) 688
BstC8I GCNNGC 4 cut(s) 46, 93, 1019, 1056
BstDEI CTNAG 7 cut(s) 131, 762, 842, 1093, 1143, 1201, 1467
BstDSI CCRYGG 2 cut(s) 406, 1440
BstENI CCTNNNNNAGG 1 cut(s) 650
BstF5I GGATG 4 cut(s) 932, 1190, 1387, 1481
BstH2I RGCGCY 2 cut(s) 92, 98
BstHHI GCGC 4 cut(s) 53, 91, 97, 714
BstKTI GATC 3 cut(s) 322, 852, 1085
BstMAI GTCTC 2 cut(s) 733, 1529
BstMBI GATC 3 cut(s) 319, 849, 1082
BstMCI CGRYCG 2 cut(s) 45, 177
BstMWI GCNNNNNNNGC 7 cut(s) 50, 59, 562, 711, 833, 1018, 1274
BstNI CCWGG 3 cut(s) 59, 184, 1068
BstSCI CCNGG 7 cut(s) 57, 159, 171, 182, 1066, 1398, 1399
BstV2I GAAGAC 2 cut(s) 596, 1242
BstX2I RGATCY 1 cut(s) 1082
BstXI CCANNNNNNTGG 2 cut(s) 330, 1306
BstYI RGATCY 1 cut(s) 1082
BstZI CGGCCG 1 cut(s) 42
BsuRI GGCC 2 cut(s) 44, 486
BtgI CCRYGG 2 cut(s) 406, 1440
BtsCI GGATG 4 cut(s) 932, 1190, 1387, 1481
BtsIMutI CAGTG 3 cut(s) 943, 955, 1014
BveI ACCTGC 1 cut(s) 1268
Cac8I GCNNGC 4 cut(s) 46, 93, 1019, 1056
CciI TCATGA 1 cut(s) 852
CfoI GCGC 4 cut(s) 53, 91, 97, 714
Cfr10I RCCGGY 2 cut(s) 44, 91
Cfr13I GGNCC 3 cut(s) 238, 994, 1294
Cfr9I CCCGGG 1 cut(s) 1399
CseI GACGC 1 cut(s) 590
Csp6I GTAC 3 cut(s) 142, 253, 689
CviQI GTAC 3 cut(s) 142, 253, 689
DdeI CTNAG 7 cut(s) 131, 762, 842, 1093, 1143, 1201, 1467
DinI GGCGCC 2 cut(s) 90, 96
DpnI GATC 3 cut(s) 321, 851, 1084
DpnII GATC 3 cut(s) 319, 849, 1082
EaeI YGGCCR 1 cut(s) 42
EagI CGGCCG 1 cut(s) 42
Eam1104I CTCTTC 3 cut(s) 489, 990, 1113
EarI CTCTTC 3 cut(s) 489, 990, 1113
EciI GGCGGA 1 cut(s) 77
EclXI CGGCCG 1 cut(s) 42
Eco130I CCWWGG 2 cut(s) 406, 1440
Eco24I GRGCYC 1 cut(s) 191
Eco31I GGTCTC 1 cut(s) 1529
Eco47I GGWCC 3 cut(s) 238, 994, 1294
Eco52I CGGCCG 1 cut(s) 42
Eco57I CTGAAG 7 cut(s) 590, 741, 971, 990, 1236, 1254, 1293
Eco88I CYCGRG 2 cut(s) 488, 1399
EcoNI CCTNNNNNAGG 1 cut(s) 650
EcoO109I RGGNCCY 2 cut(s) 238, 994
EcoRI GAATTC 1 cut(s) 448
EcoRII CCWGG 3 cut(s) 57, 182, 1066
EcoT14I CCWWGG 2 cut(s) 406, 1440
EcoT22I ATGCAT 1 cut(s) 663
EcoT38I GRGCYC 1 cut(s) 191
EgeI GGCGCC 2 cut(s) 90, 96
EheI GGCGCC 2 cut(s) 90, 96
ErhI CCWWGG 2 cut(s) 406, 1440
FaqI GGGAC 6 cut(s) 251, 530, 980, 1108, 1493, 1533
FauI CCCGC 5 cut(s) 126, 228, 741, 1005, 1047
FblI GTMKAC 1 cut(s) 569
FokI GGATG 4 cut(s) 919, 1177, 1394, 1488
FriOI GRGCYC 1 cut(s) 191
FspBI CTAG 2 cut(s) 234, 276
GlaI GCGC 4 cut(s) 52, 90, 96, 713
GsaI CCCAGC 1 cut(s) 334
GsuI CTGGAG 1 cut(s) 205
HaeII RGCGCY 2 cut(s) 92, 98
HaeIII GGCC 2 cut(s) 44, 486
HapII CCGG 8 cut(s) 45, 86, 92, 161, 173, 224, 1163, 1400
HgaI GACGC 1 cut(s) 590
HhaI GCGC 4 cut(s) 53, 91, 97, 714
Hin1I GRCGYC 2 cut(s) 89, 95
Hin6I GCGC 4 cut(s) 51, 89, 95, 712
HinP1I GCGC 4 cut(s) 51, 89, 95, 712
HincII GTYRAC 1 cut(s) 727
HindII GTYRAC 1 cut(s) 727
HinfI GANTC 5 cut(s) 65, 881, 982, 1121, 1418
HpaII CCGG 8 cut(s) 45, 86, 92, 161, 173, 224, 1163, 1400
HphI GGTGA 2 cut(s) 929, 1292
Hpy166II GTNNAC 4 cut(s) 154, 398, 570, 727
Hpy188I TCNGA 9 cut(s) 901, 1036, 1096, 1146, 1195, 1273, 1354, 1506, 1534
Hpy188III TCNNGA 9 cut(s) 130, 323, 785, 853, 910, 975, 989, 1163, 1214
Hpy8I GTNNAC 4 cut(s) 154, 398, 570, 727
Hpy99I CGWCG 2 cut(s) 32, 1354
HpyAV CCTTC 3 cut(s) 746, 1032, 1190
HpyCH4III ACNGT 2 cut(s) 950, 1075
HpyCH4IV ACGT 1 cut(s) 140
HpyCH4V TGCA 9 cut(s) 535, 598, 650, 661, 941, 1012, 1028, 1061, 1430
HpyF10VI GCNNNNNNNGC 7 cut(s) 50, 59, 562, 711, 833, 1018, 1274
HpyF3I CTNAG 7 cut(s) 131, 762, 842, 1093, 1143, 1201, 1467
HpySE526I ACGT 1 cut(s) 140
Hsp92I GRCGYC 2 cut(s) 89, 95
HspAI GCGC 4 cut(s) 51, 89, 95, 712
KasI GGCGCC 2 cut(s) 88, 94
Kpn2I TCCGGA 1 cut(s) 1162
KroI GCCGGC 2 cut(s) 44, 91
KroNI GCCGGC 2 cut(s) 46, 93
Kzo9I GATC 3 cut(s) 319, 849, 1082
LmnI GCTCC 5 cut(s) 88, 186, 616, 787, 1165
LweI GCATC 2 cut(s) 393, 1277
MaeI CTAG 2 cut(s) 234, 276
MaeII ACGT 1 cut(s) 140
MaeIII GTNAC 1 cut(s) 979
MalI GATC 3 cut(s) 321, 851, 1084
MboI GATC 3 cut(s) 319, 849, 1082
MboII GAAGA 9 cut(s) 325, 506, 596, 667, 951, 977, 1130, 1229, 1247
MflI RGATCY 1 cut(s) 1082
MhlI GDGCHC 1 cut(s) 191
MluCI AATT 6 cut(s) 335, 448, 683, 895, 1029, 1131
Mly113I GGCGCC 2 cut(s) 89, 95
MlyI GAGTC 2 cut(s) 976, 1130
MmeI TCCRAC 5 cut(s) 135, 858, 1093, 1228, 1332
Mph1103I ATGCAT 1 cut(s) 663
MreI CGCCGGCG 1 cut(s) 91
MroI TCCGGA 1 cut(s) 1162
MroNI GCCGGC 2 cut(s) 44, 91
MroXI GAANNNNTTC 1 cut(s) 1365
MseI TTAA 5 cut(s) 74, 378, 744, 801, 888
MspA1I CMGCKG 4 cut(s) 41, 330, 622, 1232
MspCI CTTAAG 2 cut(s) 377, 743
MspI CCGG 8 cut(s) 45, 86, 92, 161, 173, 224, 1163, 1400
MspR9I CCNGG 7 cut(s) 59, 161, 173, 184, 1068, 1400, 1401
Mva1269I GAATGC 1 cut(s) 1440
MvaI CCWGG 3 cut(s) 59, 184, 1068
MwoI GCNNNNNNNGC 7 cut(s) 50, 59, 562, 711, 833, 1018, 1274
NaeI GCCGGC 2 cut(s) 46, 93
NarI GGCGCC 2 cut(s) 89, 95
NciI CCSGG 4 cut(s) 161, 173, 1400, 1401
NcoI CCATGG 2 cut(s) 406, 1440
NdeII GATC 3 cut(s) 319, 849, 1082
NgoMIV GCCGGC 2 cut(s) 44, 91
NlaIV GGNNCC 8 cut(s) 84, 90, 96, 188, 218, 239, 996, 1296
NmuCI GTSAC 1 cut(s) 979
NsiI ATGCAT 1 cut(s) 663
PaeR7I CTCGAG 1 cut(s) 488
PagI TCATGA 1 cut(s) 852
PaqCI CACCTGC 1 cut(s) 1268
PctI GAATGC 1 cut(s) 1440
PdiI GCCGGC 2 cut(s) 46, 93
PdmI GAANNNNTTC 1 cut(s) 1365
PfeI GAWTC 3 cut(s) 65, 881, 1418
Pfl23II CGTACG 1 cut(s) 141
PflFI GACNNNGTC 2 cut(s) 173, 414
PflMI CCANNNNNTGG 2 cut(s) 440, 1495
PfoI TCCNGGA 1 cut(s) 182
PleI GAGTC 2 cut(s) 976, 1129
PluTI GGCGCC 2 cut(s) 92, 98
PpsI GAGTC 2 cut(s) 976, 1129
PpuMI RGGWCCY 2 cut(s) 238, 994
Psp5II RGGWCCY 2 cut(s) 238, 994
Psp6I CCWGG 3 cut(s) 57, 182, 1066
PspFI CCCAGC 1 cut(s) 330
PspGI CCWGG 3 cut(s) 57, 182, 1066
PspLI CGTACG 1 cut(s) 141
PspN4I GGNNCC 8 cut(s) 84, 90, 96, 188, 218, 239, 996, 1296
PspPI GGNCC 3 cut(s) 238, 994, 1294
PspPPI RGGWCCY 2 cut(s) 238, 994
PspXI VCTCGAGB 1 cut(s) 488
PsuI RGATCY 1 cut(s) 1082
PsyI GACNNNGTC 2 cut(s) 173, 414
PvuII CAGCTG 1 cut(s) 1232
RsaI GTAC 3 cut(s) 143, 254, 690
RsaNI GTAC 3 cut(s) 142, 253, 689
SaqAI TTAA 5 cut(s) 74, 378, 744, 801, 888
Sau3AI GATC 3 cut(s) 319, 849, 1082
Sau96I GGNCC 3 cut(s) 238, 994, 1294
ScaI AGTACT 1 cut(s) 254
SchI GAGTC 2 cut(s) 976, 1130
ScrFI CCNGG 7 cut(s) 59, 161, 173, 184, 1068, 1400, 1401
SduI GDGCHC 1 cut(s) 191
SfaNI GCATC 2 cut(s) 393, 1277
SfoI GGCGCC 2 cut(s) 90, 96
Sfr274I CTCGAG 1 cut(s) 488
SgrAI CRCCGGYG 1 cut(s) 91
SinI GGWCC 3 cut(s) 238, 994, 1294
SlaI CTCGAG 1 cut(s) 488
SmaI CCCGGG 1 cut(s) 1401
SmlI CTYRAG 3 cut(s) 377, 488, 743
SmoI CTYRAG 3 cut(s) 377, 488, 743
Sse9I AATT 6 cut(s) 335, 448, 683, 895, 1029, 1131
SspDI GGCGCC 2 cut(s) 88, 94
SspI AATATT 1 cut(s) 1159
SspMI CTAG 2 cut(s) 234, 276
StyD4I CCNGG 7 cut(s) 57, 159, 171, 182, 1066, 1398, 1399
StyI CCWWGG 2 cut(s) 406, 1440
TaaI ACNGT 2 cut(s) 950, 1075
TaiI ACGT 1 cut(s) 143
TaqI TCGA 1 cut(s) 489
TasI AATT 6 cut(s) 335, 448, 683, 895, 1029, 1131
TatI WGTACW 2 cut(s) 252, 688
TauI GCSGC 4 cut(s) 44, 51, 124, 625
TfiI GAWTC 3 cut(s) 65, 881, 1418
Tru1I TTAA 5 cut(s) 74, 378, 744, 801, 888
Tru9I TTAA 5 cut(s) 74, 378, 744, 801, 888
TscAI CASTG 3 cut(s) 943, 955, 1014
TseFI GTSAC 1 cut(s) 979
Tsp45I GTSAC 1 cut(s) 979
TspDTI ATGAA 7 cut(s) 833, 837, 869, 915, 1328, 1425, 1464
TspGWI ACGGA 2 cut(s) 318, 751
TspMI CCCGGG 1 cut(s) 1399
TspRI CASTG 3 cut(s) 943, 955, 1014
Tth111I GACNNNGTC 2 cut(s) 173, 414
Van91I CCANNNNNTGG 2 cut(s) 440, 1495
Vha464I CTTAAG 2 cut(s) 377, 743
VpaK11BI GGWCC 3 cut(s) 238, 994, 1294
XagI CCTNNNNNAGG 1 cut(s) 650
XapI RAATTY 2 cut(s) 335, 448
XhoI CTCGAG 1 cut(s) 488
XmaI CCCGGG 1 cut(s) 1399
XmiI GTMKAC 1 cut(s) 569
XmnI GAANNNNTTC 1 cut(s) 1365
XspI CTAG 2 cut(s) 234, 276
ZrmI AGTACT 1 cut(s) 254
Zsp2I ATGCAT 1 cut(s) 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.