Rroxscaffold_3G00247950

Heat shock factor protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0012798
Physical Location & Seq
Reverse (-)
41272336 .. 41277738
5403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00247950.1

Sequence Viewer

Length: 1413 bp
ATGGTGGACGACCCGGCGACGGACCCGATTGTGTCGTGGAGCGCCACCAACAACAGCTTCGTGGTTTGGAACCCGCCGGAGTTCGCCAGGGACCTTCTGCCCAAGTACTTCAAGCACAATAACTTCTCCAGTTTCGTCAGGCAGTTGAACACCTATGGATTTAGGAAAGTTGATCCTGACCGCTGGGAATTTGCAAATGAGGGCTTTTTGAGGGGTCAAAAACATCTTCTAAAGAGTATCAGCCGGCGAAAACCTGCCCATGGACATGGTCATCAACAGCCACAGCAGCCATCACATGGACAGAGTTCTGTAGCTGCATGTGTAGAGGTTGGGAAGTTTGGTCTCGAGGAAGAGGTTGAGAGGCTTAAAAGGGACAAGAATGTGCTTATGCAGGAACTTATCAGGTTGAGGCAGCAGCAACAGTCTACTGATAATCAGCTGCAAACAATGGTCCAACGTCTTCAAGGTATGGAGCAGCGGCAGCAACAGATGATGTCATTCCTTGCAAAGGCTGTGCAGAGCCCCGGTTTCTTGAATCAGTTTGTCCAGCAGCAAAATGATAGCAATAGGCGCATAACTGAAGCCAATAAGAAAAGGAGACTCAGGGAGGAAGGAATTGCTGAAAACGAGCGTTCTGATACTCCTGATGGACAGATTGTTAAGTATCAGCCTCCGATGAATGAAGCAGCAAAGGCAATGCTCAGGCAGATGATGAAAACAAATACTTCTTCCCAGCTGGATTCTTTTAGTAACAATCTTGAGAATTTTTTGATTGGTGAGGGTTCATCATCATCCAGTGCAGTAGACAGTGGTAGCTCTTCAAGCCATGTCTCGGGAGCTACACTTCAAGAGGTCCCGCCTACTTCAGGGCGTGGACCGTTACCTGCAATTTCAGAAATAGAATCTTCTCTTAAGGCTACAACCTCTGGAGAAGGTATGAGAGATCCATTGTCAGATATTAATGTCCTCATTGGAGCACAACAGGCACCAGCAATTCCTATTTCTCAGGCAGATGTAATCCCTGAGGTTTCTCAGGTACAAGGTATGGCACATGGAGGTTTAATTGATATTCCTGAAGGAAACACAAAACCTGAGGCAGATGTTGGATATTTTCAAGATATGGCACCTGAAGCCGGTGATGGATTTATCGACTCCACAGCATTTGATTTTATGTCCCTAGATGTTGATAGTATTCCCCCTGATGCTGACATTGACGCATTGCTTGACAATTCCAACTTGTGGGATTTTCTTGAACAAAGTCCTGTGCCAGATGAAATGGATTCGACCTCTGCAGAAGGCATGCCTGTGGGAAATGAAGTGCAGCCGACAGAGAATGGATGGGATAAAACCCAGCATATGGATAAGCTTACTGAGCAAATGGAAAATCTTAAATCAAATACTAAAGGGTTTTGA

Protein Analysis

470

Amino Acids

52.1

Weight (kDa)

4.82

Isoelectric Point (pI)

52.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 1 - 83 6.6e-27 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012798)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32330 AT1G32330
fragaria_vesca FvH4_5g22720
malus_domestica MD06G1037900.v1.1 MD16G1271200.v1.1
prunus_persica Prupe.5G031100_v2.0.a1
pyrus_communis pycom16g24070
rosa_chinensis RchiOBHm_Chr7g0211291
rosa_laevigata RLG00000003012
rosa_multiflora Rmu_co8434101.1_g000001 Rmu_sc0001226.1_g000001
rosa_roxburghii Rroxscaffold_3G00247950
rosa_rugosa Rorug07G0124900
rosa_samantha Rh7AG257200 Rh7BG251300 Rh7CG274000 Rh7DG264600
rosa_wichuraiana Rw7G021980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 262, 892
AccB1I GGYRCC 2 cut(s) 985, 1123
AccB7I CCANNNNNTGG 3 cut(s) 296, 1239, 1357
AccI GTMKAC 2 cut(s) 425, 804
AciI CCGC 4 cut(s) 74, 181, 478, 857
AclWI GGATC 2 cut(s) 167, 938
AcsI RAATTY 2 cut(s) 188, 763
AcuI CTGAAG 4 cut(s) 600, 849, 1095, 1149
AfaI GTAC 2 cut(s) 107, 1038
AfiI CCNNNNNNNGG 9 cut(s) 19, 260, 296, 508, 832, 866, 1133, 1239, 1357
AflII CTTAAG 1 cut(s) 911
AgsI TTSAA 8 cut(s) 112, 148, 464, 535, 822, 848, 1115, 1253
AjnI CCWGG 1 cut(s) 86
AjuI GAANNNNNNNTTGG 2 cut(s) 41, 73
AluBI AGCT 7 cut(s) 57, 314, 439, 736, 816, 839, 1366
AluI AGCT 7 cut(s) 57, 314, 439, 736, 816, 839, 1366
Alw21I GWGCWC 1 cut(s) 979
Alw26I GTCTC 3 cut(s) 347, 592, 835
AlwI GGATC 2 cut(s) 167, 938
Ama87I CYCGRG 2 cut(s) 344, 832
ApoI RAATTY 2 cut(s) 188, 763
AseI ATTAAT 1 cut(s) 960
AspLEI GCGC 2 cut(s) 44, 573
AspS9I GGNCC 5 cut(s) 22, 91, 451, 853, 875
AsuC2I CCSGG 2 cut(s) 14, 525
AsuHPI GGTGA 2 cut(s) 788, 1148
AvaI CYCGRG 2 cut(s) 344, 832
AvaII GGWCC 5 cut(s) 22, 91, 451, 853, 875
AxyI CCTNAGG 2 cut(s) 1023, 1092
BanI GGYRCC 2 cut(s) 985, 1123
BanII GRGCYC 1 cut(s) 524
BbsI GAAGAC 1 cut(s) 452
Bbv12I GWGCWC 1 cut(s) 979
BccI CCATC 4 cut(s) 298, 641, 1133, 1332
BciT130I CCWGG 1 cut(s) 88
BcnI CCSGG 2 cut(s) 14, 525
BcoDI GTCTC 3 cut(s) 347, 592, 835
BfaI CTAG 1 cut(s) 1178
BfmI CTRYAG 2 cut(s) 309, 1290
BfoI RGCGCY 1 cut(s) 45
BfrI CTTAAG 1 cut(s) 911
BfuAI ACCTGC 2 cut(s) 262, 892
BmcAI AGTACT 1 cut(s) 107
Bme1390I CCNGG 3 cut(s) 14, 88, 525
Bme18I GGWCC 5 cut(s) 22, 91, 451, 853, 875
BmeT110I CYCGRG 2 cut(s) 344, 832
BmgT120I GGNCC 5 cut(s) 22, 91, 451, 853, 875
BmiI GGNNCC 6 cut(s) 24, 71, 92, 855, 987, 1125
BmrFI CCNGG 3 cut(s) 14, 88, 525
BmsI GCATC 1 cut(s) 1192
BpiI GAAGAC 1 cut(s) 452
BpmI CTGGAG 2 cut(s) 112, 948
Bpu10I CCTNAGC 1 cut(s) 701
BpuEI CTTGAG 1 cut(s) 779
BpuMI CCSGG 2 cut(s) 14, 525
BsaI GGTCTC 1 cut(s) 347
BsaJI CCNNGG 3 cut(s) 87, 259, 523
Bsc4I CCNNNNNNNGG 9 cut(s) 19, 260, 296, 508, 832, 866, 1133, 1239, 1357
Bse118I RCCGGY 2 cut(s) 243, 1133
Bse1I ACTGG 2 cut(s) 129, 795
Bse21I CCTNAGG 2 cut(s) 1023, 1092
Bse3DI GCAATG 2 cut(s) 702, 1217
BseBI CCWGG 1 cut(s) 88
BseDI CCNNGG 3 cut(s) 87, 259, 523
BseGI GGATG 2 cut(s) 791, 1343
BseLI CCNNNNNNNGG 9 cut(s) 19, 260, 296, 508, 832, 866, 1133, 1239, 1357
BseMI GCAATG 2 cut(s) 702, 1217
BseMII CTCAG 7 cut(s) 616, 715, 1014, 1019, 1046, 1083, 1362
BseNI ACTGG 2 cut(s) 129, 795
BseYI CCCAGC 3 cut(s) 183, 732, 1350
BsgI GTGCAG 3 cut(s) 536, 819, 1340
BshNI GGYRCC 2 cut(s) 985, 1123
BsiHKAI GWGCWC 1 cut(s) 979
BsiHKCI CYCGRG 2 cut(s) 344, 832
BsiSI CCGG 5 cut(s) 14, 77, 244, 525, 1134
BslFI GGGAC 4 cut(s) 104, 386, 839, 1159
BslI CCNNNNNNNGG 9 cut(s) 19, 260, 296, 508, 832, 866, 1133, 1239, 1357
BsmAI GTCTC 3 cut(s) 347, 592, 835
BsmFI GGGAC 4 cut(s) 104, 386, 839, 1159
Bso31I GGTCTC 1 cut(s) 347
BsoBI CYCGRG 2 cut(s) 344, 832
Bsp1286I GDGCHC 2 cut(s) 524, 979
Bsp143I GATC 2 cut(s) 172, 943
Bsp19I CCATGG 1 cut(s) 259
BspACI CCGC 4 cut(s) 74, 181, 478, 857
BspCNI CTCAG 7 cut(s) 615, 714, 1015, 1018, 1045, 1084, 1363
BspLI GGNNCC 6 cut(s) 24, 71, 92, 855, 987, 1125
BspMAI CTGCAG 1 cut(s) 1294
BspMI ACCTGC 2 cut(s) 262, 892
BspPI GGATC 2 cut(s) 167, 938
BspQI GCTCTTC 1 cut(s) 823
BspT107I GGYRCC 2 cut(s) 985, 1123
BspTI CTTAAG 1 cut(s) 911
BspTNI GGTCTC 1 cut(s) 347
BsrDI GCAATG 2 cut(s) 702, 1217
BsrFI RCCGGY 2 cut(s) 243, 1133
BsrI ACTGG 2 cut(s) 129, 795
BssAI RCCGGY 2 cut(s) 243, 1133
BssECI CCNNGG 3 cut(s) 87, 259, 523
BssMI GATC 2 cut(s) 172, 943
BssT1I CCWWGG 1 cut(s) 259
Bst2UI CCWGG 1 cut(s) 88
Bst4CI ACNGT 3 cut(s) 423, 809, 879
Bst6I CTCTTC 2 cut(s) 345, 823
BstAFI CTTAAG 1 cut(s) 911
BstC8I GCNNGC 2 cut(s) 245, 1301
BstDEI CTNAG 7 cut(s) 602, 701, 1005, 1023, 1032, 1092, 1371
BstDSI CCRYGG 1 cut(s) 259
BstENI CCTNNNNNAGG 2 cut(s) 506, 864
BstF5I GGATG 2 cut(s) 791, 1343
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 2 cut(s) 44, 573
BstKTI GATC 2 cut(s) 175, 946
BstMAI GTCTC 3 cut(s) 347, 592, 835
BstMBI GATC 2 cut(s) 172, 943
BstMWI GCNNNNNNNGC 8 cut(s) 286, 481, 570, 692, 822, 983, 1130, 1372
BstNI CCWGG 1 cut(s) 88
BstNSI RCATGY 2 cut(s) 321, 1303
BstSCI CCNGG 3 cut(s) 12, 86, 523
BstSFI CTRYAG 2 cut(s) 309, 1290
BstV2I GAAGAC 1 cut(s) 452
BstX2I RGATCY 1 cut(s) 943
BstXI CCANNNNNNTGG 1 cut(s) 266
BstYI RGATCY 1 cut(s) 943
Bsu36I CCTNAGG 2 cut(s) 1023, 1092
BtgI CCRYGG 1 cut(s) 259
BtsCI GGATG 2 cut(s) 791, 1343
BtsIMutI CAGTG 2 cut(s) 802, 814
BveI ACCTGC 2 cut(s) 262, 892
Cac8I GCNNGC 2 cut(s) 245, 1301
CfoI GCGC 2 cut(s) 44, 573
Cfr10I RCCGGY 2 cut(s) 243, 1133
Cfr13I GGNCC 5 cut(s) 22, 91, 451, 853, 875
CseI GACGC 1 cut(s) 1223
Csp6I GTAC 2 cut(s) 106, 1037
CspCI CAANNNNNGTGG 2 cut(s) 1144, 1179
CviAII CATG 7 cut(s) 260, 266, 296, 318, 827, 1052, 1300
CviQI GTAC 2 cut(s) 106, 1037
DdeI CTNAG 7 cut(s) 602, 701, 1005, 1023, 1032, 1092, 1371
DpnI GATC 2 cut(s) 174, 945
DpnII GATC 2 cut(s) 172, 943
Eam1104I CTCTTC 2 cut(s) 345, 823
EarI CTCTTC 2 cut(s) 345, 823
Eco130I CCWWGG 1 cut(s) 259
Eco24I GRGCYC 1 cut(s) 524
Eco31I GGTCTC 1 cut(s) 347
Eco47I GGWCC 5 cut(s) 22, 91, 451, 853, 875
Eco57I CTGAAG 4 cut(s) 600, 849, 1095, 1149
Eco81I CCTNAGG 2 cut(s) 1023, 1092
Eco88I CYCGRG 2 cut(s) 344, 832
EcoNI CCTNNNNNAGG 2 cut(s) 506, 864
EcoO109I RGGNCCY 2 cut(s) 91, 853
EcoRII CCWGG 1 cut(s) 86
EcoT14I CCWWGG 1 cut(s) 259
EcoT38I GRGCYC 1 cut(s) 524
ErhI CCWWGG 1 cut(s) 259
FaeI CATG 7 cut(s) 263, 269, 299, 321, 830, 1055, 1303
FaqI GGGAC 4 cut(s) 104, 386, 839, 1159
FatI CATG 7 cut(s) 259, 265, 295, 317, 826, 1051, 1299
FauI CCCGC 2 cut(s) 81, 864
FauNDI CATATG 1 cut(s) 1356
FblI GTMKAC 2 cut(s) 425, 804
FokI GGATG 2 cut(s) 778, 1350
FriOI GRGCYC 1 cut(s) 524
FspBI CTAG 1 cut(s) 1178
GlaI GCGC 2 cut(s) 43, 572
GsaI CCCAGC 3 cut(s) 187, 736, 1354
GsuI CTGGAG 2 cut(s) 112, 948
HaeII RGCGCY 1 cut(s) 45
HapII CCGG 5 cut(s) 14, 77, 244, 525, 1134
HgaI GACGC 1 cut(s) 1223
HhaI GCGC 2 cut(s) 44, 573
Hin1II CATG 7 cut(s) 263, 269, 299, 321, 830, 1055, 1303
Hin6I GCGC 2 cut(s) 42, 571
HinP1I GCGC 2 cut(s) 42, 571
HindIII AAGCTT 1 cut(s) 1364
HinfI GANTC 6 cut(s) 535, 600, 740, 902, 1151, 1280
HpaII CCGG 5 cut(s) 14, 77, 244, 525, 1134
HphI GGTGA 2 cut(s) 788, 1148
Hpy166II GTNNAC 4 cut(s) 7, 426, 805, 875
Hpy188I TCNGA 4 cut(s) 637, 675, 895, 955
Hpy8I GTNNAC 4 cut(s) 7, 426, 805, 875
Hpy99I CGWCG 1 cut(s) 22
HpyAV CCTTC 5 cut(s) 104, 605, 926, 1070, 1289
HpyCH4III ACNGT 3 cut(s) 423, 809, 879
HpyCH4IV ACGT 1 cut(s) 457
HpyF10VI GCNNNNNNNGC 8 cut(s) 286, 481, 570, 692, 822, 983, 1130, 1372
HpyF3I CTNAG 7 cut(s) 602, 701, 1005, 1023, 1032, 1092, 1371
HpySE526I ACGT 1 cut(s) 457
Hsp92II CATG 7 cut(s) 263, 269, 299, 321, 830, 1055, 1303
HspAI GCGC 2 cut(s) 42, 571
KroI GCCGGC 1 cut(s) 243
KroNI GCCGGC 1 cut(s) 245
Kzo9I GATC 2 cut(s) 172, 943
LguI GCTCTTC 1 cut(s) 823
LmnI GCTCC 4 cut(s) 39, 472, 836, 974
LweI GCATC 1 cut(s) 1192
MaeI CTAG 1 cut(s) 1178
MaeII ACGT 1 cut(s) 457
MaeIII GTNAC 2 cut(s) 749, 879
MalI GATC 2 cut(s) 174, 945
MboI GATC 2 cut(s) 172, 943
MboII GAAGA 6 cut(s) 218, 362, 452, 720, 810, 897
MflI RGATCY 1 cut(s) 943
MhlI GDGCHC 2 cut(s) 524, 979
MluCI AATT 7 cut(s) 188, 615, 763, 888, 993, 1062, 1228
MlyI GAGTC 2 cut(s) 594, 1145
MmeI TCCRAC 3 cut(s) 478, 1084, 1257
MroNI GCCGGC 1 cut(s) 243
MseI TTAA 6 cut(s) 366, 660, 912, 960, 1061, 1389
MslI CAYNNNNRTG 2 cut(s) 264, 1304
MspA1I CMGCKG 4 cut(s) 183, 439, 478, 736
MspCI CTTAAG 1 cut(s) 911
MspI CCGG 5 cut(s) 14, 77, 244, 525, 1134
MspR9I CCNGG 3 cut(s) 14, 88, 525
MvaI CCWGG 1 cut(s) 88
MwoI GCNNNNNNNGC 8 cut(s) 286, 481, 570, 692, 822, 983, 1130, 1372
NaeI GCCGGC 1 cut(s) 245
NciI CCSGG 2 cut(s) 14, 525
NcoI CCATGG 1 cut(s) 259
NdeI CATATG 1 cut(s) 1356
NdeII GATC 2 cut(s) 172, 943
NgoMIV GCCGGC 1 cut(s) 243
NlaIII CATG 7 cut(s) 263, 269, 299, 321, 830, 1055, 1303
NlaIV GGNNCC 6 cut(s) 24, 71, 92, 855, 987, 1125
NspI RCATGY 2 cut(s) 321, 1303
PaeI GCATGC 1 cut(s) 1303
PaeR7I CTCGAG 1 cut(s) 344
PciSI GCTCTTC 1 cut(s) 823
PdiI GCCGGC 1 cut(s) 245
PfeI GAWTC 4 cut(s) 535, 740, 902, 1280
PflFI GACNNNGTC 1 cut(s) 267
PflMI CCANNNNNTGG 3 cut(s) 296, 1239, 1357
PleI GAGTC 2 cut(s) 594, 1145
PpsI GAGTC 2 cut(s) 594, 1145
PpuMI RGGWCCY 2 cut(s) 91, 853
PshBI ATTAAT 1 cut(s) 960
Psp5II RGGWCCY 2 cut(s) 91, 853
Psp6I CCWGG 1 cut(s) 86
PspFI CCCAGC 3 cut(s) 183, 732, 1350
PspGI CCWGG 1 cut(s) 86
PspN4I GGNNCC 6 cut(s) 24, 71, 92, 855, 987, 1125
PspPI GGNCC 5 cut(s) 22, 91, 451, 853, 875
PspPPI RGGWCCY 2 cut(s) 91, 853
PstI CTGCAG 1 cut(s) 1294
PsuI RGATCY 1 cut(s) 943
PsyI GACNNNGTC 1 cut(s) 267
PvuII CAGCTG 2 cut(s) 439, 736
RsaI GTAC 2 cut(s) 107, 1038
RsaNI GTAC 2 cut(s) 106, 1037
RseI CAYNNNNRTG 2 cut(s) 264, 1304
SapI GCTCTTC 1 cut(s) 823
SaqAI TTAA 6 cut(s) 366, 660, 912, 960, 1061, 1389
Sau3AI GATC 2 cut(s) 172, 943
Sau96I GGNCC 5 cut(s) 22, 91, 451, 853, 875
ScaI AGTACT 1 cut(s) 107
SchI GAGTC 2 cut(s) 594, 1145
ScrFI CCNGG 3 cut(s) 14, 88, 525
SduI GDGCHC 2 cut(s) 524, 979
SfaNI GCATC 1 cut(s) 1192
SfcI CTRYAG 2 cut(s) 309, 1290
Sfr274I CTCGAG 1 cut(s) 344
SinI GGWCC 5 cut(s) 22, 91, 451, 853, 875
SlaI CTCGAG 1 cut(s) 344
SmiMI CAYNNNNRTG 2 cut(s) 264, 1304
SmlI CTYRAG 3 cut(s) 344, 758, 911
SmoI CTYRAG 3 cut(s) 344, 758, 911
SphI GCATGC 1 cut(s) 1303
Sse9I AATT 7 cut(s) 188, 615, 763, 888, 993, 1062, 1228
SsiI CCGC 4 cut(s) 74, 181, 478, 857
SspMI CTAG 1 cut(s) 1178
StyD4I CCNGG 3 cut(s) 12, 86, 523
StyI CCWWGG 1 cut(s) 259
TaaI ACNGT 3 cut(s) 423, 809, 879
TaiI ACGT 1 cut(s) 460
TaqI TCGA 3 cut(s) 345, 1149, 1283
TasI AATT 7 cut(s) 188, 615, 763, 888, 993, 1062, 1228
TatI WGTACW 1 cut(s) 105
TauI GCSGC 1 cut(s) 481
TfiI GAWTC 4 cut(s) 535, 740, 902, 1280
Tru1I TTAA 6 cut(s) 366, 660, 912, 960, 1061, 1389
Tru9I TTAA 6 cut(s) 366, 660, 912, 960, 1061, 1389
TscAI CASTG 2 cut(s) 802, 814
TspDTI ATGAA 6 cut(s) 692, 696, 728, 774, 1287, 1329
TspGWI ACGGA 1 cut(s) 35
TspRI CASTG 2 cut(s) 802, 814
Tth111I GACNNNGTC 1 cut(s) 267
Van91I CCANNNNNTGG 3 cut(s) 296, 1239, 1357
Vha464I CTTAAG 1 cut(s) 911
VpaK11BI GGWCC 5 cut(s) 22, 91, 451, 853, 875
VspI ATTAAT 1 cut(s) 960
XagI CCTNNNNNAGG 2 cut(s) 506, 864
XapI RAATTY 2 cut(s) 188, 763
XceI RCATGY 2 cut(s) 321, 1303
XhoI CTCGAG 1 cut(s) 344
XmiI GTMKAC 2 cut(s) 425, 804
XspI CTAG 1 cut(s) 1178
ZrmI AGTACT 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.