MD17G1029700.v1.1

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0017392
Physical Location & Seq
Reverse (-)
2138449 .. 2141710
3262 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1029700.v1.1.491

Sequence Viewer

Length: 951 bp
ATGGGATACGTAGAGGAAATGGAATCCTTGTCCAAAGCAATTAGATACGACGATCATGGAGAACCAACTGTGGATTTTTATACGTCATGGCTGACAAAGCATCCATCTGCTTTGGATGCTTTTGGACAGATGATGAGCGCAGTGAAGGGAAAAGATATCGTTGTGTTTCTGGATTATGACGGCACTCTCTCCCGTATCGTGCAAGATCCTGATAAAGCTTTTATGACGGCCAAGATGCGTTCAGCAGTAGATGAAGTTGCCTGTTGTTTCCCGACTGCAATAATCTCTGGAAGACGTCGAGATAAAGTTTATGATTTTGTGAAATTAGACAATATCTACTATGCTGGAAGCCACGGGATGGATATATCGACCCCTTCAGGCTCTTCAAAATACAGCTATCACAAGCATCAATTTAGAAGCACTGATGCAAAAGGAAATGAATATGTTAACTTTCGTCCAGCATTCGAGTTTCTGCCTACCATCGCAAAGATAATAAAGCTGTTAAACGAAGAAACAAAAGGTATAAAAGGTGCCGTGGTTGAAGATAATAAATTCTGCATCTCTGTTCACTACCGATGCGTCAAAGAAGAGGAAGTAGACACACTTAAAGGGATGGTGGAAACTCTGATGAAAAATTACAAAAACTTTCGCATATCAGGAGGAAAAAAGGTTATGGAAATACGCCCAAAAATTGACTGGAACAAAGGTCGAGCCTTGCAATATTTGCTTGACACACCAGGGTTCGGCAGCTCCACTGATGTCCTCCCAATCTATATTGGAGATGACGAAACTGATGAAGATGCTTTTGAGGCTATTAGGAGAATTGGAAGAGGCTTCCCTATTGTTGTTTCATCCACTCCAAAGGAGACCAAGGCTTTGTACTCGTTGCGTGACCCAACGGAAGTCACGAGTTTCTTGTCATGTCTTGCTGCATGGAGGAAAGACACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Trehalose_PPase

Protein Analysis

317

Amino Acids

35.73

Weight (kDa)

6.34

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 56 - 299 1.5e-60 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017392)

Species Orthologous Gene IDs
malus_domestica MD17G1029700.v1.1
pyrus_communis pycom17g02670
rosa_chinensis RchiOBHm_Chr2g0172991
rosa_laevigata RLG00000022147
rosa_multiflora Rmu_sc0012743.1_g000008 Rmu_sc0032348.1_g000001
rosa_roxburghii Rroxscaffold_2G00079370
rosa_rugosa Rorug02G0564300
rosa_samantha Rh2AG640900 Rh2BG654600 Rh2CG619900 Rh2DG667900
rosa_wichuraiana Rw2G052900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 298
AccB1I GGYRCC 1 cut(s) 530
AccB7I CCANNNNNTGG 1 cut(s) 358
AccI GTMKAC 1 cut(s) 597
AclWI GGATC 1 cut(s) 200
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 1 cut(s) 551
AcuI CTGAAG 1 cut(s) 360
AcyI GRCGYC 1 cut(s) 295
AfaI GTAC 1 cut(s) 881
AfiI CCNNNNNNNGG 2 cut(s) 358, 743
AgsI TTSAA 2 cut(s) 387, 542
AjnI CCWGG 1 cut(s) 736
AluBI AGCT 4 cut(s) 218, 396, 499, 750
AluI AGCT 4 cut(s) 218, 396, 499, 750
Alw26I GTCTC 1 cut(s) 860
AlwI GGATC 1 cut(s) 200
AoxI GGCC 1 cut(s) 228
ApeKI GCWGC 2 cut(s) 747, 929
ApoI RAATTY 1 cut(s) 551
ArsI GACNNNNNNTTYG 2 cut(s) 859, 891
AspLEI GCGC 1 cut(s) 140
BanI GGYRCC 1 cut(s) 530
BauI CACGAG 1 cut(s) 907
BbsI GAAGAC 1 cut(s) 298
BbvI GCAGC 2 cut(s) 759, 916
BccI CCATC 4 cut(s) 112, 352, 488, 607
BceAI ACGGC 3 cut(s) 196, 243, 518
BciT130I CCWGG 1 cut(s) 738
BcoDI GTCTC 1 cut(s) 860
BisI GCNGC 2 cut(s) 748, 930
BlsI GCNGC 2 cut(s) 749, 931
Bme1390I CCNGG 1 cut(s) 738
BmiI GGNNCC 1 cut(s) 532
BmrFI CCNGG 1 cut(s) 738
BmsI GCATC 8 cut(s) 106, 109, 225, 415, 415, 566, 567, 790
BpiI GAAGAC 1 cut(s) 298
BsaAI YACGTR 1 cut(s) 10
BsaHI GRCGYC 1 cut(s) 295
BsaI GGTCTC 1 cut(s) 860
BsaJI CCNNGG 4 cut(s) 352, 534, 737, 870
Bsc4I CCNNNNNNNGG 2 cut(s) 358, 743
Bse1I ACTGG 1 cut(s) 701
BseBI CCWGG 1 cut(s) 738
BseDI CCNNGG 4 cut(s) 352, 534, 737, 870
BseGI GGATG 5 cut(s) 100, 121, 363, 618, 851
BseLI CCNNNNNNNGG 2 cut(s) 358, 743
BseNI ACTGG 1 cut(s) 701
BseXI GCAGC 2 cut(s) 759, 916
BshFI GGCC 1 cut(s) 230
BshNI GGYRCC 1 cut(s) 530
BslI CCNNNNNNNGG 2 cut(s) 358, 743
BsmAI GTCTC 1 cut(s) 860
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 1 cut(s) 230
Bso31I GGTCTC 1 cut(s) 860
Bsp143I GATC 2 cut(s) 52, 205
BspANI GGCC 1 cut(s) 230
BspLI GGNNCC 1 cut(s) 532
BspPI GGATC 1 cut(s) 200
BspQI GCTCTTC 1 cut(s) 388
BspT107I GGYRCC 1 cut(s) 530
BspTNI GGTCTC 1 cut(s) 860
BsrI ACTGG 1 cut(s) 701
BssECI CCNNGG 4 cut(s) 352, 534, 737, 870
BssMI GATC 2 cut(s) 52, 205
BssNI GRCGYC 1 cut(s) 295
BssSI CACGAG 1 cut(s) 907
BssT1I CCWWGG 1 cut(s) 870
Bst2BI CACGAG 1 cut(s) 907
Bst2UI CCWGG 1 cut(s) 738
Bst4CI ACNGT 1 cut(s) 70
Bst6I CTCTTC 3 cut(s) 388, 582, 823
BstACI GRCGYC 1 cut(s) 295
BstAPI GCANNNNNTGC 1 cut(s) 724
BstBAI YACGTR 1 cut(s) 10
BstDSI CCRYGG 2 cut(s) 352, 534
BstF5I GGATG 5 cut(s) 100, 121, 363, 618, 851
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 2 cut(s) 55, 208
BstMAI GTCTC 1 cut(s) 860
BstMBI GATC 2 cut(s) 52, 205
BstMWI GCNNNNNNNGC 4 cut(s) 97, 116, 724, 809
BstNI CCWGG 1 cut(s) 738
BstSCI CCNGG 1 cut(s) 736
BstSNI TACGTA 1 cut(s) 10
BstV1I GCAGC 2 cut(s) 759, 916
BstV2I GAAGAC 1 cut(s) 298
BstX2I RGATCY 1 cut(s) 205
BstYI RGATCY 1 cut(s) 205
BsuRI GGCC 1 cut(s) 230
BtgI CCRYGG 2 cut(s) 352, 534
BtgZI GCGATG 1 cut(s) 466
BtsCI GGATG 5 cut(s) 100, 121, 363, 618, 851
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 3 cut(s) 147, 420, 753
CfoI GCGC 1 cut(s) 140
CseI GACGC 1 cut(s) 568
Csp6I GTAC 1 cut(s) 880
CviAII CATG 4 cut(s) 56, 87, 921, 933
CviQI GTAC 1 cut(s) 880
DpnI GATC 2 cut(s) 54, 207
DpnII GATC 2 cut(s) 52, 205
EaeI YGGCCR 1 cut(s) 228
Eam1104I CTCTTC 3 cut(s) 388, 582, 823
EarI CTCTTC 3 cut(s) 388, 582, 823
Eco105I TACGTA 1 cut(s) 10
Eco130I CCWWGG 1 cut(s) 870
Eco31I GGTCTC 1 cut(s) 860
Eco32I GATATC 1 cut(s) 157
Eco57I CTGAAG 1 cut(s) 360
EcoRII CCWGG 1 cut(s) 736
EcoRV GATATC 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 870
ErhI CCWWGG 1 cut(s) 870
FaeI CATG 4 cut(s) 59, 90, 924, 936
FatI CATG 4 cut(s) 55, 86, 920, 932
FblI GTMKAC 1 cut(s) 597
Fnu4HI GCNGC 2 cut(s) 748, 930
FokI GGATG 5 cut(s) 87, 128, 370, 625, 838
Fsp4HI GCNGC 2 cut(s) 748, 930
GlaI GCGC 1 cut(s) 139
GluI GCNGC 2 cut(s) 748, 930
HaeIII GGCC 1 cut(s) 230
HgaI GACGC 1 cut(s) 568
HhaI GCGC 1 cut(s) 140
Hin1I GRCGYC 1 cut(s) 295
Hin1II CATG 4 cut(s) 59, 90, 924, 936
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HindIII AAGCTT 1 cut(s) 216
HinfI GANTC 1 cut(s) 23
HpaI GTTAAC 1 cut(s) 448
Hpy166II GTNNAC 3 cut(s) 448, 568, 598
Hpy188I TCNGA 1 cut(s) 627
Hpy188III TCNNGA 7 cut(s) 170, 209, 271, 288, 299, 657, 907
Hpy8I GTNNAC 3 cut(s) 448, 568, 598
Hpy99I CGWCG 2 cut(s) 53, 300
HpyAV CCTTC 2 cut(s) 139, 384
HpyCH4III ACNGT 1 cut(s) 70
HpyCH4IV ACGT 3 cut(s) 9, 83, 295
HpyCH4V TGCA 6 cut(s) 202, 278, 428, 558, 718, 932
HpyF10VI GCNNNNNNNGC 4 cut(s) 97, 116, 724, 809
HpySE526I ACGT 3 cut(s) 9, 83, 295
Hsp92I GRCGYC 1 cut(s) 295
Hsp92II CATG 4 cut(s) 59, 90, 924, 936
HspAI GCGC 1 cut(s) 138
KspAI GTTAAC 1 cut(s) 448
Kzo9I GATC 2 cut(s) 52, 205
LguI GCTCTTC 1 cut(s) 388
LmnI GCTCC 1 cut(s) 755
Lsp1109I GCAGC 2 cut(s) 759, 916
LweI GCATC 8 cut(s) 106, 109, 225, 415, 415, 566, 567, 790
MaeII ACGT 3 cut(s) 9, 83, 295
MaeIII GTNAC 2 cut(s) 890, 904
MalI GATC 2 cut(s) 54, 207
MboI GATC 2 cut(s) 52, 205
MboII GAAGA 7 cut(s) 303, 375, 521, 554, 599, 809, 840
MflI RGATCY 1 cut(s) 205
MluCI AATT 7 cut(s) 39, 323, 410, 551, 634, 690, 822
MnlI CCTC 7 cut(s) 7, 583, 653, 773, 802, 824, 930
MseI TTAA 3 cut(s) 447, 503, 606
MspR9I CCNGG 1 cut(s) 738
Mva1269I GAATGC 1 cut(s) 461
MvaI CCWGG 1 cut(s) 738
MwoI GCNNNNNNNGC 4 cut(s) 97, 116, 724, 809
NdeII GATC 2 cut(s) 52, 205
NlaIII CATG 4 cut(s) 59, 90, 924, 936
NlaIV GGNNCC 1 cut(s) 532
NmuCI GTSAC 2 cut(s) 890, 904
PciSI GCTCTTC 1 cut(s) 388
PcsI WCGNNNNNNNCGW 1 cut(s) 905
PctI GAATGC 1 cut(s) 461
PfeI GAWTC 1 cut(s) 23
PflMI CCANNNNNTGG 1 cut(s) 358
PkrI GCNGC 2 cut(s) 749, 931
Ppu21I YACGTR 1 cut(s) 10
Psp6I CCWGG 1 cut(s) 736
PspGI CCWGG 1 cut(s) 736
PspN4I GGNNCC 1 cut(s) 532
PsuI RGATCY 1 cut(s) 205
RsaI GTAC 1 cut(s) 881
RsaNI GTAC 1 cut(s) 880
SapI GCTCTTC 1 cut(s) 388
SaqAI TTAA 3 cut(s) 447, 503, 606
SatI GCNGC 2 cut(s) 748, 930
Sau3AI GATC 2 cut(s) 52, 205
ScrFI CCNGG 1 cut(s) 738
SfaNI GCATC 8 cut(s) 106, 109, 225, 415, 415, 566, 567, 790
SnaBI TACGTA 1 cut(s) 10
Sse9I AATT 7 cut(s) 39, 323, 410, 551, 634, 690, 822
SspI AATATT 1 cut(s) 722
StyD4I CCNGG 1 cut(s) 736
StyI CCWWGG 1 cut(s) 870
TaaI ACNGT 1 cut(s) 70
TaiI ACGT 3 cut(s) 12, 86, 298
TaqI TCGA 4 cut(s) 298, 368, 465, 709
TasI AATT 7 cut(s) 39, 323, 410, 551, 634, 690, 822
TatI WGTACW 1 cut(s) 879
TfiI GAWTC 1 cut(s) 23
Tru1I TTAA 3 cut(s) 447, 503, 606
Tru9I TTAA 3 cut(s) 447, 503, 606
TscAI CASTG 3 cut(s) 147, 427, 760
TseFI GTSAC 2 cut(s) 890, 904
TseI GCWGC 2 cut(s) 747, 929
Tsp45I GTSAC 2 cut(s) 890, 904
TspDTI ATGAA 5 cut(s) 267, 453, 644, 810, 840
TspGWI ACGGA 1 cut(s) 914
TspRI CASTG 3 cut(s) 147, 427, 760
Van91I CCANNNNNTGG 1 cut(s) 358
XapI RAATTY 1 cut(s) 551
XcmI CCANNNNNNNNNTGG 1 cut(s) 693
XmiI GTMKAC 1 cut(s) 597
ZraI GACGTC 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.