Rh2BG654600

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0017392
Physical Location & Seq
Reverse (-)
87092312 .. 87094113
1802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG654600.1

Sequence Viewer

Length: 975 bp
ATGGGACACCAAGATGAAACGGAGTCCTGGAAAGCAAGAGGCCACGAAGATAATAGAGAACCAACTGATGATGTGGGATCTCATACTTCATGGCTGGCAAAGCATCCATCTGCTTTACATGGTTTTGAAGACATGATGATGACGAGCATGGCAGTTGCCAAGGGAAAGAAAATCGTCGTCTTGTTGGATTACGACGGTACTCTTTCCCATATCGTACCAGACCCTGATCAAGCTTTCATGACTGAAGCAATGCGATCAGCCGTAGATCAAGTTGCTAACTGTTTCCCTACTGCAATCATTTCTGGAAGGCGTCGAGATAAGGTCCATGACTTTGTGAAATTAGACAATCTCTGTTATGCTGGAAGCCACGGCTTGGATATATCGACCCCATCAGCCTCTTCAAAATATAGTAACCATAAGCATCAATCTAGAACCACTGATGGAAAAGGAAATGAATCCGATGTCTTTCGTCCAGCCGTAGATTTTTTGCCCACAATTAAAAAGATAAAGAAGGCACTGGAAGAAGAAATAAAGGGTATAGAAGGTGCCATGATAGAAGATAATACATTCTGCATCTCTGTGCACTATCGGTGCGTCAAAGAAGAGAAGGTGGACAAACTTAAAGGGACGGTTGAATCTTTCATGGAAGCTTACGATAACTTTCGCATATCGGAAGGGAAAAAGGTTATGGAAATACGCCCTAAGATCGACTGGGACAAAGGTCGGGCCTTGCAATATTTGCTAGACACACCCGATTTCGGCAGCTCAAGTAATGATGTTATCCCAGTGTTTATAGGAGATGACAAAACTGATGAAGATGCTTTTACGGCTATAAAGACAATGGGGCGAGGTATTCCCATTGTTGTTTCTTCCACTCCAAGGGAGACCGAAGCATCCTACTCGTTACGTGATCCAACGGAAGTCATGCGTTTCCTATTACAACTTGCGAAGTGGACGGAAGAAGGCTCCGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Trehalose_PPase

Protein Analysis

324

Amino Acids

36.27

Weight (kDa)

5.43

Isoelectric Point (pI)

32.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 61 - 305 1.8e-59 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017392)

Species Orthologous Gene IDs
malus_domestica MD17G1029700.v1.1
pyrus_communis pycom17g02670
rosa_chinensis RchiOBHm_Chr2g0172991
rosa_laevigata RLG00000022147
rosa_multiflora Rmu_sc0012743.1_g000008 Rmu_sc0032348.1_g000001
rosa_roxburghii Rroxscaffold_2G00079370
rosa_rugosa Rorug02G0564300
rosa_samantha Rh2AG640900 Rh2BG654600 Rh2CG619900 Rh2DG667900
rosa_wichuraiana Rw2G052900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 545
AccB7I CCANNNNNTGG 1 cut(s) 373
AclWI GGATC 2 cut(s) 85, 905
AcuI CTGAAG 1 cut(s) 264
AcyI GRCGYC 1 cut(s) 310
AfaI GTAC 2 cut(s) 199, 216
AfiI CCNNNNNNNGG 3 cut(s) 373, 758, 879
AgsI TTSAA 3 cut(s) 128, 402, 635
AjnI CCWGG 1 cut(s) 26
AluBI AGCT 3 cut(s) 233, 650, 765
AluI AGCT 3 cut(s) 233, 650, 765
Alw21I GWGCWC 1 cut(s) 585
Alw26I GTCTC 1 cut(s) 878
Alw44I GTGCAC 1 cut(s) 581
AlwI GGATC 2 cut(s) 85, 905
AlwNI CAGNNNCTG 1 cut(s) 224
AoxI GGCC 2 cut(s) 40, 726
ApaLI GTGCAC 1 cut(s) 581
ApeKI GCWGC 1 cut(s) 762
AspS9I GGNCC 2 cut(s) 322, 726
AvaII GGWCC 1 cut(s) 322
BaeGI GKGCMC 1 cut(s) 585
BanI GGYRCC 1 cut(s) 545
BbsI GAAGAC 1 cut(s) 135
Bbv12I GWGCWC 1 cut(s) 585
BbvI GCAGC 1 cut(s) 774
BccI CCATC 3 cut(s) 115, 397, 434
BceAI ACGGC 4 cut(s) 245, 385, 461, 843
BcgI CGANNNNNNTGC 2 cut(s) 882, 916
BciT130I CCWGG 1 cut(s) 28
BclI TGATCA 1 cut(s) 226
BcoDI GTCTC 1 cut(s) 878
BfaI CTAG 2 cut(s) 429, 743
BisI GCNGC 1 cut(s) 763
BlsI GCNGC 1 cut(s) 764
Bme1390I CCNGG 1 cut(s) 28
Bme18I GGWCC 1 cut(s) 322
BmgT120I GGNCC 2 cut(s) 322, 726
BmiI GGNNCC 2 cut(s) 547, 967
BmrFI CCNGG 1 cut(s) 28
BmrI ACTGGG 2 cut(s) 721, 779
BmsI GCATC 5 cut(s) 112, 430, 582, 808, 902
BmuI ACTGGG 2 cut(s) 721, 779
BoxI GACNNNNGTC 1 cut(s) 720
BpiI GAAGAC 1 cut(s) 135
BpuEI CTTGAG 1 cut(s) 751
BsaAI YACGTR 1 cut(s) 908
BsaHI GRCGYC 1 cut(s) 310
BsaI GGTCTC 1 cut(s) 878
BsaJI CCNNGG 3 cut(s) 159, 367, 878
Bsc4I CCNNNNNNNGG 3 cut(s) 373, 758, 879
Bse1I ACTGG 3 cut(s) 522, 716, 785
Bse3DI GCAATG 1 cut(s) 255
BseBI CCWGG 1 cut(s) 28
BseDI CCNNGG 3 cut(s) 159, 367, 878
BseGI GGATG 2 cut(s) 103, 893
BseLI CCNNNNNNNGG 3 cut(s) 373, 758, 879
BseMI GCAATG 1 cut(s) 255
BseNI ACTGG 3 cut(s) 522, 716, 785
BseSI GKGCMC 1 cut(s) 585
BseXI GCAGC 1 cut(s) 774
BshFI GGCC 2 cut(s) 42, 728
BshNI GGYRCC 1 cut(s) 545
BsiHKAI GWGCWC 1 cut(s) 585
BslFI GGGAC 3 cut(s) 18, 640, 728
BslI CCNNNNNNNGG 3 cut(s) 373, 758, 879
BsmAI GTCTC 1 cut(s) 878
BsmFI GGGAC 3 cut(s) 18, 640, 728
BsnI GGCC 2 cut(s) 42, 728
Bso31I GGTCTC 1 cut(s) 878
Bsp1286I GDGCHC 1 cut(s) 585
Bsp143I GATC 6 cut(s) 77, 226, 254, 265, 705, 910
BspANI GGCC 2 cut(s) 42, 728
BspHI TCATGA 1 cut(s) 237
BspLI GGNNCC 2 cut(s) 547, 967
BspPI GGATC 2 cut(s) 85, 905
BspT107I GGYRCC 1 cut(s) 545
BspTNI GGTCTC 1 cut(s) 878
BsrDI GCAATG 1 cut(s) 255
BsrI ACTGG 3 cut(s) 522, 716, 785
BssECI CCNNGG 3 cut(s) 159, 367, 878
BssMI GATC 6 cut(s) 77, 226, 254, 265, 705, 910
BssNI GRCGYC 1 cut(s) 310
BssT1I CCWWGG 2 cut(s) 159, 878
Bst2UI CCWGG 1 cut(s) 28
Bst4CI ACNGT 3 cut(s) 197, 281, 631
Bst6I CTCTTC 2 cut(s) 403, 597
BstACI GRCGYC 1 cut(s) 310
BstAPI GCANNNNNTGC 1 cut(s) 739
BstBAI YACGTR 1 cut(s) 908
BstC8I GCNNGC 1 cut(s) 96
BstDEI CTNAG 1 cut(s) 702
BstDSI CCRYGG 1 cut(s) 367
BstF5I GGATG 2 cut(s) 103, 893
BstKTI GATC 6 cut(s) 80, 229, 257, 268, 708, 913
BstMAI GTCTC 1 cut(s) 878
BstMBI GATC 6 cut(s) 77, 226, 254, 265, 705, 910
BstMWI GCNNNNNNNGC 3 cut(s) 100, 739, 827
BstNI CCWGG 1 cut(s) 28
BstPAI GACNNNNGTC 1 cut(s) 720
BstSCI CCNGG 1 cut(s) 26
BstSLI GKGCMC 1 cut(s) 585
BstV1I GCAGC 1 cut(s) 774
BstV2I GAAGAC 1 cut(s) 135
BstX2I RGATCY 1 cut(s) 77
BstYI RGATCY 1 cut(s) 77
BsuRI GGCC 2 cut(s) 42, 728
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 2 cut(s) 103, 893
BtsIMutI CAGTG 3 cut(s) 435, 515, 792
Cac8I GCNNGC 1 cut(s) 96
CaiI CAGNNNCTG 1 cut(s) 224
CciI TCATGA 1 cut(s) 237
Cfr13I GGNCC 2 cut(s) 322, 726
CseI GACGC 2 cut(s) 299, 583
Csp6I GTAC 2 cut(s) 198, 215
CviAII CATG 9 cut(s) 90, 119, 133, 148, 238, 326, 550, 643, 925
CviQI GTAC 2 cut(s) 198, 215
DdeI CTNAG 1 cut(s) 702
DpnI GATC 6 cut(s) 79, 228, 256, 267, 707, 912
DpnII GATC 6 cut(s) 77, 226, 254, 265, 705, 910
Eam1104I CTCTTC 2 cut(s) 403, 597
EarI CTCTTC 2 cut(s) 403, 597
Eco130I CCWWGG 2 cut(s) 159, 878
Eco31I GGTCTC 1 cut(s) 878
Eco47I GGWCC 1 cut(s) 322
Eco57I CTGAAG 1 cut(s) 264
EcoRII CCWGG 1 cut(s) 26
EcoT14I CCWWGG 2 cut(s) 159, 878
ErhI CCWWGG 2 cut(s) 159, 878
FaeI CATG 9 cut(s) 93, 122, 136, 151, 241, 329, 553, 646, 928
FaqI GGGAC 3 cut(s) 18, 640, 728
FatI CATG 9 cut(s) 89, 118, 132, 147, 237, 325, 549, 642, 924
FbaI TGATCA 1 cut(s) 226
Fnu4HI GCNGC 1 cut(s) 763
FokI GGATG 2 cut(s) 90, 880
Fsp4HI GCNGC 1 cut(s) 763
FspBI CTAG 2 cut(s) 429, 743
GluI GCNGC 1 cut(s) 763
HaeIII GGCC 2 cut(s) 42, 728
HgaI GACGC 2 cut(s) 299, 583
Hin1I GRCGYC 1 cut(s) 310
Hin1II CATG 9 cut(s) 93, 122, 136, 151, 241, 329, 553, 646, 928
HindIII AAGCTT 2 cut(s) 231, 648
HinfI GANTC 3 cut(s) 23, 455, 635
Hpy166II GTNNAC 3 cut(s) 583, 613, 954
Hpy188I TCNGA 2 cut(s) 460, 673
Hpy188III TCNNGA 4 cut(s) 238, 303, 314, 429
Hpy8I GTNNAC 3 cut(s) 583, 613, 954
Hpy99I CGWCG 3 cut(s) 179, 197, 315
HpyAV CCTTC 6 cut(s) 300, 505, 536, 601, 668, 956
HpyCH4III ACNGT 3 cut(s) 197, 281, 631
HpyCH4IV ACGT 1 cut(s) 907
HpyCH4V TGCA 4 cut(s) 293, 573, 583, 733
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 739, 827
HpyF3I CTNAG 1 cut(s) 702
HpySE526I ACGT 1 cut(s) 907
Hsp92I GRCGYC 1 cut(s) 310
Hsp92II CATG 9 cut(s) 93, 122, 136, 151, 241, 329, 553, 646, 928
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 6 cut(s) 77, 226, 254, 265, 705, 910
LmnI GCTCC 1 cut(s) 971
Lsp1109I GCAGC 1 cut(s) 774
LweI GCATC 5 cut(s) 112, 430, 582, 808, 902
MaeI CTAG 2 cut(s) 429, 743
MaeII ACGT 1 cut(s) 907
MaeIII GTNAC 2 cut(s) 410, 903
MalI GATC 6 cut(s) 79, 228, 256, 267, 707, 912
MboI GATC 6 cut(s) 77, 226, 254, 265, 705, 910
MflI RGATCY 1 cut(s) 77
MhlI GDGCHC 1 cut(s) 585
MluCI AATT 2 cut(s) 338, 495
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 2 cut(s) 165, 938
MnlI CCTC 3 cut(s) 32, 406, 842
MseI TTAA 2 cut(s) 498, 621
MslI CAYNNNNRTG 3 cut(s) 12, 137, 578
MspR9I CCNGG 1 cut(s) 28
MvaI CCWGG 1 cut(s) 28
MwoI GCNNNNNNNGC 3 cut(s) 100, 739, 827
NdeII GATC 6 cut(s) 77, 226, 254, 265, 705, 910
NlaIII CATG 9 cut(s) 93, 122, 136, 151, 241, 329, 553, 646, 928
NlaIV GGNNCC 2 cut(s) 547, 967
PagI TCATGA 1 cut(s) 237
PfeI GAWTC 2 cut(s) 455, 635
PflMI CCANNNNNTGG 1 cut(s) 373
PfoI TCCNGGA 1 cut(s) 26
PkrI GCNGC 1 cut(s) 764
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
Ppu21I YACGTR 1 cut(s) 908
PshAI GACNNNNGTC 1 cut(s) 720
Psp6I CCWGG 1 cut(s) 26
PspGI CCWGG 1 cut(s) 26
PspN4I GGNNCC 2 cut(s) 547, 967
PspPI GGNCC 2 cut(s) 322, 726
PstNI CAGNNNCTG 1 cut(s) 224
PsuI RGATCY 1 cut(s) 77
RsaI GTAC 2 cut(s) 199, 216
RsaNI GTAC 2 cut(s) 198, 215
RseI CAYNNNNRTG 3 cut(s) 12, 137, 578
SaqAI TTAA 2 cut(s) 498, 621
SatI GCNGC 1 cut(s) 763
Sau3AI GATC 6 cut(s) 77, 226, 254, 265, 705, 910
Sau96I GGNCC 2 cut(s) 322, 726
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 1 cut(s) 28
SduI GDGCHC 1 cut(s) 585
SfaNI GCATC 5 cut(s) 112, 430, 582, 808, 902
SinI GGWCC 1 cut(s) 322
SmiMI CAYNNNNRTG 3 cut(s) 12, 137, 578
SmlI CTYRAG 1 cut(s) 766
SmoI CTYRAG 1 cut(s) 766
Sse9I AATT 2 cut(s) 338, 495
SspI AATATT 1 cut(s) 737
SspMI CTAG 2 cut(s) 429, 743
StyD4I CCNGG 1 cut(s) 26
StyI CCWWGG 2 cut(s) 159, 878
TaaI ACNGT 3 cut(s) 197, 281, 631
TaiI ACGT 1 cut(s) 910
TaqI TCGA 3 cut(s) 313, 383, 708
TaqII GACCGA 1 cut(s) 902
TasI AATT 2 cut(s) 338, 495
TfiI GAWTC 2 cut(s) 455, 635
Tru1I TTAA 2 cut(s) 498, 621
Tru9I TTAA 2 cut(s) 498, 621
TscAI CASTG 3 cut(s) 442, 522, 792
TseI GCWGC 1 cut(s) 762
TspDTI ATGAA 6 cut(s) 30, 78, 226, 468, 631, 828
TspGWI ACGGA 4 cut(s) 35, 932, 958, 971
TspRI CASTG 3 cut(s) 442, 522, 792
Van91I CCANNNNNTGG 1 cut(s) 373
VneI GTGCAC 1 cut(s) 581
VpaK11BI GGWCC 1 cut(s) 322
XbaI TCTAGA 1 cut(s) 428
XspI CTAG 2 cut(s) 429, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.