Rw2G052900

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0017392
Physical Location & Seq
Reverse (-)
80797085 .. 80798904
1820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G052900.1

Sequence Viewer

Length: 975 bp
ATGGGACACCAAGATGAAACGGAGTCCTGGAAAGCAAGAGGCCACGAAGATAATAGAGAACCAACTGATGATGTGGGATCTCATACTTCATGGCTGGAAAAGCATCCATCTGCTTTAGATGGTTTTGAAGACATGATGATGACGAGCATGGCAGCTGCCAAGGGAAAGAAACTTGTCGTCTTGTTGGATTACGACGGTACTCTTTCCCATATCGTTCCAGACCCTGATCAAGCTTTCATGACTGAAGCAATGCGATCAGCCGTAGATCAAGTTGCTAACTGTTTTCCTACTGCAATCATTTCTGGAAGGCGTCGAGATAAGGTCTATGACTTTGTGAAATTAGACAATCTCTGTTATGCTGGAAGCCACGGCTTGGATATATCGACCCCAGCAGCCTCTTCAAAATATAGTAACCATAAGCATCAATCTAGAACCACTGATGGAAAAGGAAATGAATCCGACGTCTTTCGTCCAGCCGTAGATTTTTTGCCCACCATTAAAAAGATAAAGAAGGCACTGGAAGAAGAAATAAAGGGTATAGAAGGTGCCATGATAGAAGATAATACATTCTGCATCTCTGTGCACTATCGGTGCGTCAAAGAAGAGAAGGTGGACAATCTTAAAGGGACAGTTGAATCTTTCATGAAAGCTTACGATAACTTTCGCATATCGGAAGGGAAAAAGGTTATGGAAATACGCCCTAAGATCGACTGGGACAAAGGTCGGGCCTTGCAATATTTGCTAGACACACCCGATTTCGGCAGCTCAAGTAATGATGTTATCCCAGTGTTTATAGGAGATGACAAAACTGATGAAGATGCTTTTAAGGCTATTAAGACAATTGGGCGAGGTATTCCCATTGTTGTTTCTTCCACTCCAAAGGAGACCGAAGCATCCTACTCGTTACGTGATCCAACGGAAGTCATGCGTTTCCTGTTACAACTTGCAAAGTGGATGGAAGAAGGCTCCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Trehalose_PPase

Protein Analysis

324

Amino Acids

36.28

Weight (kDa)

5.3

Isoelectric Point (pI)

34.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 61 - 305 4.4e-60 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017392)

Species Orthologous Gene IDs
malus_domestica MD17G1029700.v1.1
pyrus_communis pycom17g02670
rosa_chinensis RchiOBHm_Chr2g0172991
rosa_laevigata RLG00000022147
rosa_multiflora Rmu_sc0012743.1_g000008 Rmu_sc0032348.1_g000001
rosa_roxburghii Rroxscaffold_2G00079370
rosa_rugosa Rorug02G0564300
rosa_samantha Rh2AG640900 Rh2BG654600 Rh2CG619900 Rh2DG667900
rosa_wichuraiana Rw2G052900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 465
AccB1I GGYRCC 1 cut(s) 545
AccB7I CCANNNNNTGG 1 cut(s) 373
AclWI GGATC 2 cut(s) 85, 905
AcuI CTGAAG 1 cut(s) 264
AcyI GRCGYC 2 cut(s) 310, 462
AfaI GTAC 1 cut(s) 199
AfiI CCNNNNNNNGG 2 cut(s) 373, 758
AgsI TTSAA 3 cut(s) 128, 402, 635
AjnI CCWGG 1 cut(s) 26
AluBI AGCT 4 cut(s) 155, 233, 650, 765
AluI AGCT 4 cut(s) 155, 233, 650, 765
Alw21I GWGCWC 1 cut(s) 585
Alw26I GTCTC 1 cut(s) 878
Alw44I GTGCAC 1 cut(s) 581
AlwI GGATC 2 cut(s) 85, 905
AlwNI CAGNNNCTG 1 cut(s) 224
AoxI GGCC 2 cut(s) 40, 726
ApaLI GTGCAC 1 cut(s) 581
ApeKI GCWGC 4 cut(s) 152, 155, 392, 762
AspS9I GGNCC 1 cut(s) 726
BaeGI GKGCMC 1 cut(s) 585
BanI GGYRCC 1 cut(s) 545
BbsI GAAGAC 1 cut(s) 135
Bbv12I GWGCWC 1 cut(s) 585
BbvI GCAGC 4 cut(s) 142, 164, 404, 774
BccI CCATC 4 cut(s) 113, 115, 434, 949
BceAI ACGGC 3 cut(s) 245, 385, 461
BcgI CGANNNNNNTGC 2 cut(s) 882, 916
BciT130I CCWGG 1 cut(s) 28
BclI TGATCA 1 cut(s) 226
BcoDI GTCTC 1 cut(s) 878
BfaI CTAG 2 cut(s) 429, 743
BisI GCNGC 4 cut(s) 153, 156, 393, 763
BlsI GCNGC 4 cut(s) 154, 157, 394, 764
Bme1390I CCNGG 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 726
BmiI GGNNCC 2 cut(s) 547, 967
BmrFI CCNGG 1 cut(s) 28
BmrI ACTGGG 2 cut(s) 721, 779
BmsI GCATC 5 cut(s) 112, 430, 582, 808, 902
BmuI ACTGGG 2 cut(s) 721, 779
BoxI GACNNNNGTC 1 cut(s) 720
BpiI GAAGAC 1 cut(s) 135
BpuEI CTTGAG 1 cut(s) 751
BsaAI YACGTR 1 cut(s) 908
BsaHI GRCGYC 2 cut(s) 310, 462
BsaI GGTCTC 1 cut(s) 878
BsaJI CCNNGG 2 cut(s) 159, 367
Bsc4I CCNNNNNNNGG 2 cut(s) 373, 758
Bse1I ACTGG 3 cut(s) 522, 716, 785
Bse3DI GCAATG 1 cut(s) 255
BseBI CCWGG 1 cut(s) 28
BseDI CCNNGG 2 cut(s) 159, 367
BseGI GGATG 3 cut(s) 103, 893, 960
BseLI CCNNNNNNNGG 2 cut(s) 373, 758
BseMI GCAATG 1 cut(s) 255
BseNI ACTGG 3 cut(s) 522, 716, 785
BseSI GKGCMC 1 cut(s) 585
BseXI GCAGC 4 cut(s) 142, 164, 404, 774
BseYI CCCAGC 1 cut(s) 388
BshFI GGCC 2 cut(s) 42, 728
BshNI GGYRCC 1 cut(s) 545
BsiHKAI GWGCWC 1 cut(s) 585
BslFI GGGAC 3 cut(s) 18, 640, 728
BslI CCNNNNNNNGG 2 cut(s) 373, 758
BsmAI GTCTC 1 cut(s) 878
BsmFI GGGAC 3 cut(s) 18, 640, 728
BsnI GGCC 2 cut(s) 42, 728
Bso31I GGTCTC 1 cut(s) 878
Bsp1286I GDGCHC 1 cut(s) 585
Bsp143I GATC 6 cut(s) 77, 226, 254, 265, 705, 910
BspANI GGCC 2 cut(s) 42, 728
BspHI TCATGA 2 cut(s) 237, 642
BspLI GGNNCC 2 cut(s) 547, 967
BspPI GGATC 2 cut(s) 85, 905
BspT107I GGYRCC 1 cut(s) 545
BspTNI GGTCTC 1 cut(s) 878
BsrDI GCAATG 1 cut(s) 255
BsrI ACTGG 3 cut(s) 522, 716, 785
BssECI CCNNGG 2 cut(s) 159, 367
BssMI GATC 6 cut(s) 77, 226, 254, 265, 705, 910
BssNI GRCGYC 2 cut(s) 310, 462
BssT1I CCWWGG 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 28
Bst4CI ACNGT 3 cut(s) 197, 281, 631
Bst6I CTCTTC 2 cut(s) 403, 597
BstACI GRCGYC 2 cut(s) 310, 462
BstAPI GCANNNNNTGC 1 cut(s) 739
BstBAI YACGTR 1 cut(s) 908
BstDEI CTNAG 1 cut(s) 702
BstDSI CCRYGG 1 cut(s) 367
BstF5I GGATG 3 cut(s) 103, 893, 960
BstKTI GATC 6 cut(s) 80, 229, 257, 268, 708, 913
BstMAI GTCTC 1 cut(s) 878
BstMBI GATC 6 cut(s) 77, 226, 254, 265, 705, 910
BstMWI GCNNNNNNNGC 3 cut(s) 100, 739, 827
BstNI CCWGG 1 cut(s) 28
BstPAI GACNNNNGTC 1 cut(s) 720
BstSCI CCNGG 1 cut(s) 26
BstSLI GKGCMC 1 cut(s) 585
BstV1I GCAGC 4 cut(s) 142, 164, 404, 774
BstV2I GAAGAC 1 cut(s) 135
BstX2I RGATCY 1 cut(s) 77
BstYI RGATCY 1 cut(s) 77
BsuRI GGCC 2 cut(s) 42, 728
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 3 cut(s) 103, 893, 960
BtsIMutI CAGTG 3 cut(s) 435, 515, 792
CaiI CAGNNNCTG 1 cut(s) 224
CciI TCATGA 2 cut(s) 237, 642
Cfr13I GGNCC 1 cut(s) 726
CseI GACGC 2 cut(s) 299, 583
Csp6I GTAC 1 cut(s) 198
CviAII CATG 7 cut(s) 90, 133, 148, 238, 550, 643, 925
CviQI GTAC 1 cut(s) 198
DdeI CTNAG 1 cut(s) 702
DpnI GATC 6 cut(s) 79, 228, 256, 267, 707, 912
DpnII GATC 6 cut(s) 77, 226, 254, 265, 705, 910
Eam1104I CTCTTC 2 cut(s) 403, 597
EarI CTCTTC 2 cut(s) 403, 597
Eco130I CCWWGG 1 cut(s) 159
Eco31I GGTCTC 1 cut(s) 878
Eco57I CTGAAG 1 cut(s) 264
EcoRII CCWGG 1 cut(s) 26
EcoT14I CCWWGG 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 7 cut(s) 93, 136, 151, 241, 553, 646, 928
FaqI GGGAC 3 cut(s) 18, 640, 728
FatI CATG 7 cut(s) 89, 132, 147, 237, 549, 642, 924
FbaI TGATCA 1 cut(s) 226
Fnu4HI GCNGC 4 cut(s) 153, 156, 393, 763
FokI GGATG 3 cut(s) 90, 880, 967
Fsp4HI GCNGC 4 cut(s) 153, 156, 393, 763
FspBI CTAG 2 cut(s) 429, 743
GluI GCNGC 4 cut(s) 153, 156, 393, 763
GsaI CCCAGC 1 cut(s) 392
HaeIII GGCC 2 cut(s) 42, 728
HgaI GACGC 2 cut(s) 299, 583
Hin1I GRCGYC 2 cut(s) 310, 462
Hin1II CATG 7 cut(s) 93, 136, 151, 241, 553, 646, 928
HindIII AAGCTT 2 cut(s) 231, 648
HinfI GANTC 3 cut(s) 23, 455, 635
Hpy166II GTNNAC 2 cut(s) 583, 613
Hpy188I TCNGA 2 cut(s) 460, 673
Hpy188III TCNNGA 6 cut(s) 218, 238, 303, 314, 429, 643
Hpy8I GTNNAC 2 cut(s) 583, 613
Hpy99I CGWCG 3 cut(s) 197, 315, 464
HpyAV CCTTC 6 cut(s) 300, 505, 536, 601, 668, 956
HpyCH4III ACNGT 3 cut(s) 197, 281, 631
HpyCH4IV ACGT 2 cut(s) 462, 907
HpyCH4V TGCA 5 cut(s) 293, 573, 583, 733, 947
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 739, 827
HpyF3I CTNAG 1 cut(s) 702
HpySE526I ACGT 2 cut(s) 462, 907
Hsp92I GRCGYC 2 cut(s) 310, 462
Hsp92II CATG 7 cut(s) 93, 136, 151, 241, 553, 646, 928
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 6 cut(s) 77, 226, 254, 265, 705, 910
LmnI GCTCC 1 cut(s) 971
Lsp1109I GCAGC 4 cut(s) 142, 164, 404, 774
LweI GCATC 5 cut(s) 112, 430, 582, 808, 902
MaeI CTAG 2 cut(s) 429, 743
MaeII ACGT 2 cut(s) 462, 907
MaeIII GTNAC 3 cut(s) 410, 903, 936
MalI GATC 6 cut(s) 79, 228, 256, 267, 707, 912
MboI GATC 6 cut(s) 77, 226, 254, 265, 705, 910
MfeI CAATTG 1 cut(s) 840
MflI RGATCY 1 cut(s) 77
MhlI GDGCHC 1 cut(s) 585
MluCI AATT 2 cut(s) 338, 840
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 3 cut(s) 165, 483, 938
MnlI CCTC 3 cut(s) 32, 406, 842
MseI TTAA 4 cut(s) 498, 621, 825, 834
MslI CAYNNNNRTG 3 cut(s) 12, 137, 578
MspA1I CMGCKG 1 cut(s) 155
MspR9I CCNGG 1 cut(s) 28
MunI CAATTG 1 cut(s) 840
MvaI CCWGG 1 cut(s) 28
MwoI GCNNNNNNNGC 3 cut(s) 100, 739, 827
NdeII GATC 6 cut(s) 77, 226, 254, 265, 705, 910
NlaIII CATG 7 cut(s) 93, 136, 151, 241, 553, 646, 928
NlaIV GGNNCC 2 cut(s) 547, 967
PagI TCATGA 2 cut(s) 237, 642
PfeI GAWTC 2 cut(s) 455, 635
PflMI CCANNNNNTGG 1 cut(s) 373
PfoI TCCNGGA 1 cut(s) 26
PkrI GCNGC 4 cut(s) 154, 157, 394, 764
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
Ppu21I YACGTR 1 cut(s) 908
PshAI GACNNNNGTC 1 cut(s) 720
Psp6I CCWGG 1 cut(s) 26
PspFI CCCAGC 1 cut(s) 388
PspGI CCWGG 1 cut(s) 26
PspN4I GGNNCC 2 cut(s) 547, 967
PspPI GGNCC 1 cut(s) 726
PstNI CAGNNNCTG 1 cut(s) 224
PsuI RGATCY 1 cut(s) 77
PvuII CAGCTG 1 cut(s) 155
RsaI GTAC 1 cut(s) 199
RsaNI GTAC 1 cut(s) 198
RseI CAYNNNNRTG 3 cut(s) 12, 137, 578
SaqAI TTAA 4 cut(s) 498, 621, 825, 834
SatI GCNGC 4 cut(s) 153, 156, 393, 763
Sau3AI GATC 6 cut(s) 77, 226, 254, 265, 705, 910
Sau96I GGNCC 1 cut(s) 726
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 1 cut(s) 28
SduI GDGCHC 1 cut(s) 585
SfaNI GCATC 5 cut(s) 112, 430, 582, 808, 902
SmiMI CAYNNNNRTG 3 cut(s) 12, 137, 578
SmlI CTYRAG 1 cut(s) 766
SmoI CTYRAG 1 cut(s) 766
Sse9I AATT 2 cut(s) 338, 840
SspI AATATT 1 cut(s) 737
SspMI CTAG 2 cut(s) 429, 743
StyD4I CCNGG 1 cut(s) 26
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 3 cut(s) 197, 281, 631
TaiI ACGT 2 cut(s) 465, 910
TaqI TCGA 3 cut(s) 313, 383, 708
TaqII GACCGA 1 cut(s) 902
TasI AATT 2 cut(s) 338, 840
TfiI GAWTC 2 cut(s) 455, 635
Tru1I TTAA 4 cut(s) 498, 621, 825, 834
Tru9I TTAA 4 cut(s) 498, 621, 825, 834
TscAI CASTG 3 cut(s) 442, 522, 792
TseI GCWGC 4 cut(s) 152, 155, 392, 762
TspDTI ATGAA 7 cut(s) 30, 78, 226, 468, 631, 659, 828
TspGWI ACGGA 3 cut(s) 35, 932, 958
TspRI CASTG 3 cut(s) 442, 522, 792
Van91I CCANNNNNTGG 1 cut(s) 373
VneI GTGCAC 1 cut(s) 581
XbaI TCTAGA 1 cut(s) 428
XspI CTAG 2 cut(s) 429, 743
ZraI GACGTC 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.