Rmu_sc0012743.1_g000008

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0012743.1
OG0017392
Physical Location & Seq
Forward (+)
37594 .. 40229
2636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0012743.1_g000008.1.cds

Sequence Viewer

Length: 1104 bp
atgggacaccaagatgaaacggagtcctggaaagcaagaggccacgaagataatagagaaacaactgatgatgtgggatctcatacttcatggctggaaaagcatccatctgctttagatggttttgaagacatgatgatgacgagcatggcagctgccaagggaaagaaaatcgtcgtcttgttggattacgacggtactctttcccatatcgttccagaccctgatcaagctttcatgactgaagcaatgcgatcagccgtagatcaagttgctaactgttttcctactgcaatcatttctggaaggcgtcgagataaggtctatgactttgtgaaattagacaatctctgttatgctggaagccacggcttggatatatcgaccccagcagcctcttcaaaatatagtaaccataagcatcaatctagaaccactgatggaaaaataaagaaggcactggaagaagaaataaagggtatagaaggtgccatgatagaagataatacattctgcatctctgtgcactatcggtgcgtcaaagaagagaaggtggacaatcttaaagggacagttgaatctttcatgaaagcttacgataactttcgcatatcggaagggaaaaaggttatggaaatacgccctaagatcgactgggacaaaggtcgggccttgcaatatttgctagacacacccgatttcggcagctcaagtaatgatgttatcccagtgtttataggagatgacaaaactgatgaagatgcttttaaggaaattccgactggcgttagagaatcagatatcgttgtttttcactatagagctcgacgacttcccataagcctcacatggcgtgtcgccttcactcggccagcttttgaagctcgttggagatgtctgcaaggaggccaccagagatggcggcgaggctctgctgcactatcaccaggtttgagtgacgagagtcttgacaacccgaggtcacttccgttcaactctggaaagggactaggtgtcttggcattgcttgagctgatgatgaatggcaaccaaattcccaaatcccaaagagggtggctgggtttcacgtgggattgtggctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Trehalose_PPase

Protein Analysis

367

Amino Acids

41.26

Weight (kDa)

6.03

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017392)

Species Orthologous Gene IDs
malus_domestica MD17G1029700.v1.1
pyrus_communis pycom17g02670
rosa_chinensis RchiOBHm_Chr2g0172991
rosa_laevigata RLG00000022147
rosa_multiflora Rmu_sc0012743.1_g000008 Rmu_sc0032348.1_g000001
rosa_roxburghii Rroxscaffold_2G00079370
rosa_rugosa Rorug02G0564300
rosa_samantha Rh2AG640900 Rh2BG654600 Rh2CG619900 Rh2DG667900
rosa_wichuraiana Rw2G052900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 488
AccB7I CCANNNNNTGG 1 cut(s) 373
AciI CCGC 1 cut(s) 922
AclWI GGATC 1 cut(s) 85
AcoI YGGCCR 1 cut(s) 869
AcsI RAATTY 2 cut(s) 774, 1053
AcuI CTGAAG 1 cut(s) 264
AcvI CACGTG 1 cut(s) 1089
AcyI GRCGYC 1 cut(s) 310
AfaI GTAC 1 cut(s) 199
AfiI CCNNNNNNNGG 4 cut(s) 373, 701, 867, 1071
AgsI TTSAA 5 cut(s) 128, 402, 578, 881, 994
AhdI GACNNNNNGTC 1 cut(s) 1013
AjnI CCWGG 2 cut(s) 26, 946
AluBI AGCT 8 cut(s) 155, 233, 593, 708, 824, 875, 884, 1033
AluI AGCT 8 cut(s) 155, 233, 593, 708, 824, 875, 884, 1033
Alw21I GWGCWC 2 cut(s) 528, 826
Alw44I GTGCAC 1 cut(s) 524
AlwI GGATC 1 cut(s) 85
AlwNI CAGNNNCTG 1 cut(s) 224
Ama87I CYCGRG 1 cut(s) 976
AoxI GGCC 4 cut(s) 40, 669, 869, 907
ApaLI GTGCAC 1 cut(s) 524
ApeKI GCWGC 5 cut(s) 152, 155, 392, 705, 935
ApoI RAATTY 2 cut(s) 774, 1053
AspS9I GGNCC 1 cut(s) 669
AsuHPI GGTGA 1 cut(s) 936
AvaI CYCGRG 1 cut(s) 976
BaeGI GKGCMC 1 cut(s) 528
BanI GGYRCC 1 cut(s) 488
BanII GRGCYC 1 cut(s) 826
BbrPI CACGTG 1 cut(s) 1089
BbsI GAAGAC 1 cut(s) 135
Bbv12I GWGCWC 2 cut(s) 528, 826
BbvI GCAGC 5 cut(s) 142, 164, 404, 717, 922
BccI CCATC 4 cut(s) 113, 115, 434, 912
BceAI ACGGC 2 cut(s) 245, 385
BciT130I CCWGG 2 cut(s) 28, 948
BclI TGATCA 1 cut(s) 226
BfaI CTAG 4 cut(s) 429, 686, 1010, 1102
BfmI CTRYAG 1 cut(s) 817
BisI GCNGC 6 cut(s) 153, 156, 393, 706, 923, 936
BlsI GCNGC 6 cut(s) 154, 157, 394, 707, 924, 937
Bme1390I CCNGG 2 cut(s) 28, 948
BmeRI GACNNNNNGTC 1 cut(s) 1013
BmeT110I CYCGRG 1 cut(s) 976
BmgT120I GGNCC 1 cut(s) 669
BmiI GGNNCC 1 cut(s) 490
BmrFI CCNGG 2 cut(s) 28, 948
BmrI ACTGGG 2 cut(s) 664, 722
BmsI GCATC 4 cut(s) 112, 430, 525, 751
BmuI ACTGGG 2 cut(s) 664, 722
BoxI GACNNNNGTC 2 cut(s) 663, 963
BpiI GAAGAC 1 cut(s) 135
BpuEI CTTGAG 2 cut(s) 694, 1049
BsaAI YACGTR 1 cut(s) 1089
BsaHI GRCGYC 1 cut(s) 310
BsaJI CCNNGG 3 cut(s) 159, 367, 977
Bsc4I CCNNNNNNNGG 4 cut(s) 373, 701, 867, 1071
Bse1I ACTGG 4 cut(s) 465, 659, 728, 787
Bse3DI GCAATG 2 cut(s) 255, 1022
BseBI CCWGG 2 cut(s) 28, 948
BseDI CCNNGG 3 cut(s) 159, 367, 977
BseGI GGATG 1 cut(s) 103
BseLI CCNNNNNNNGG 4 cut(s) 373, 701, 867, 1071
BseMI GCAATG 2 cut(s) 255, 1022
BseNI ACTGG 4 cut(s) 465, 659, 728, 787
BseSI GKGCMC 1 cut(s) 528
BseXI GCAGC 5 cut(s) 142, 164, 404, 717, 922
BseYI CCCAGC 2 cut(s) 388, 1078
BsgI GTGCAG 1 cut(s) 921
BshFI GGCC 4 cut(s) 42, 671, 871, 909
BshNI GGYRCC 1 cut(s) 488
BsiHKAI GWGCWC 2 cut(s) 528, 826
BsiHKCI CYCGRG 1 cut(s) 976
BslFI GGGAC 4 cut(s) 18, 583, 671, 1020
BslI CCNNNNNNNGG 4 cut(s) 373, 701, 867, 1071
BsmFI GGGAC 4 cut(s) 18, 583, 671, 1020
BsnI GGCC 4 cut(s) 42, 671, 871, 909
BsoBI CYCGRG 1 cut(s) 976
Bsp1286I GDGCHC 2 cut(s) 528, 826
Bsp143I GATC 5 cut(s) 77, 226, 254, 265, 648
BspACI CCGC 1 cut(s) 922
BspANI GGCC 4 cut(s) 42, 671, 871, 909
BspHI TCATGA 2 cut(s) 237, 585
BspLI GGNNCC 1 cut(s) 490
BspPI GGATC 1 cut(s) 85
BspT107I GGYRCC 1 cut(s) 488
BsrDI GCAATG 2 cut(s) 255, 1022
BsrI ACTGG 4 cut(s) 465, 659, 728, 787
BssECI CCNNGG 3 cut(s) 159, 367, 977
BssMI GATC 5 cut(s) 77, 226, 254, 265, 648
BssNI GRCGYC 1 cut(s) 310
BssT1I CCWWGG 1 cut(s) 159
Bst2UI CCWGG 2 cut(s) 28, 948
Bst4CI ACNGT 3 cut(s) 197, 281, 574
Bst6I CTCTTC 2 cut(s) 403, 540
BstACI GRCGYC 1 cut(s) 310
BstAPI GCANNNNNTGC 1 cut(s) 682
BstBAI YACGTR 1 cut(s) 1089
BstC8I GCNNGC 1 cut(s) 873
BstDEI CTNAG 1 cut(s) 645
BstDSI CCRYGG 1 cut(s) 367
BstF5I GGATG 1 cut(s) 103
BstKTI GATC 5 cut(s) 80, 229, 257, 268, 651
BstMBI GATC 5 cut(s) 77, 226, 254, 265, 648
BstMWI GCNNNNNNNGC 3 cut(s) 100, 682, 881
BstNI CCWGG 2 cut(s) 28, 948
BstPAI GACNNNNGTC 2 cut(s) 663, 963
BstSCI CCNGG 2 cut(s) 26, 946
BstSFI CTRYAG 1 cut(s) 817
BstSLI GKGCMC 1 cut(s) 528
BstV1I GCAGC 5 cut(s) 142, 164, 404, 717, 922
BstV2I GAAGAC 1 cut(s) 135
BstX2I RGATCY 1 cut(s) 77
BstYI RGATCY 1 cut(s) 77
BsuRI GGCC 4 cut(s) 42, 671, 871, 909
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 1 cut(s) 103
BtsIMutI CAGTG 3 cut(s) 435, 458, 735
Cac8I GCNNGC 1 cut(s) 873
CaiI CAGNNNCTG 1 cut(s) 224
CciI TCATGA 2 cut(s) 237, 585
Cfr13I GGNCC 1 cut(s) 669
CseI GACGC 2 cut(s) 299, 526
CsiI ACCWGGT 1 cut(s) 946
Csp6I GTAC 1 cut(s) 198
CspCI CAANNNNNGTGG 2 cut(s) 1055, 1090
CviAII CATG 7 cut(s) 90, 133, 148, 238, 493, 586, 849
CviQI GTAC 1 cut(s) 198
DdeI CTNAG 1 cut(s) 645
DpnI GATC 5 cut(s) 79, 228, 256, 267, 650
DpnII GATC 5 cut(s) 77, 226, 254, 265, 648
DriI GACNNNNNGTC 1 cut(s) 1013
EaeI YGGCCR 1 cut(s) 869
Eam1104I CTCTTC 2 cut(s) 403, 540
Eam1105I GACNNNNNGTC 1 cut(s) 1013
EarI CTCTTC 2 cut(s) 403, 540
Ecl136II GAGCTC 1 cut(s) 824
Eco130I CCWWGG 1 cut(s) 159
Eco24I GRGCYC 1 cut(s) 826
Eco32I GATATC 1 cut(s) 802
Eco53kI GAGCTC 1 cut(s) 824
Eco57I CTGAAG 1 cut(s) 264
Eco72I CACGTG 1 cut(s) 1089
Eco88I CYCGRG 1 cut(s) 976
EcoICRI GAGCTC 1 cut(s) 824
EcoRII CCWGG 2 cut(s) 26, 946
EcoRV GATATC 1 cut(s) 802
EcoT14I CCWWGG 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 826
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 7 cut(s) 93, 136, 151, 241, 496, 589, 852
FaqI GGGAC 4 cut(s) 18, 583, 671, 1020
FatI CATG 7 cut(s) 89, 132, 147, 237, 492, 585, 848
FbaI TGATCA 1 cut(s) 226
Fnu4HI GCNGC 6 cut(s) 153, 156, 393, 706, 923, 936
FokI GGATG 1 cut(s) 90
FriOI GRGCYC 1 cut(s) 826
Fsp4HI GCNGC 6 cut(s) 153, 156, 393, 706, 923, 936
FspBI CTAG 4 cut(s) 429, 686, 1010, 1102
GluI GCNGC 6 cut(s) 153, 156, 393, 706, 923, 936
GsaI CCCAGC 2 cut(s) 392, 1082
HaeIII GGCC 4 cut(s) 42, 671, 871, 909
HgaI GACGC 2 cut(s) 299, 526
Hin1I GRCGYC 1 cut(s) 310
Hin1II CATG 7 cut(s) 93, 136, 151, 241, 496, 589, 852
HindIII AAGCTT 2 cut(s) 231, 591
HinfI GANTC 4 cut(s) 23, 578, 794, 964
HphI GGTGA 1 cut(s) 936
Hpy166II GTNNAC 2 cut(s) 526, 556
Hpy188I TCNGA 3 cut(s) 616, 780, 799
Hpy188III TCNNGA 8 cut(s) 218, 238, 303, 314, 429, 586, 968, 999
Hpy8I GTNNAC 2 cut(s) 526, 556
Hpy99I CGWCG 4 cut(s) 179, 197, 315, 831
HpyAV CCTTC 6 cut(s) 300, 448, 479, 544, 611, 871
HpyCH4III ACNGT 3 cut(s) 197, 281, 574
HpyCH4IV ACGT 1 cut(s) 1088
HpyCH4V TGCA 6 cut(s) 293, 516, 526, 676, 901, 938
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 682, 881
HpyF3I CTNAG 1 cut(s) 645
HpySE526I ACGT 1 cut(s) 1088
Hsp92I GRCGYC 1 cut(s) 310
Hsp92II CATG 7 cut(s) 93, 136, 151, 241, 496, 589, 852
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 5 cut(s) 77, 226, 254, 265, 648
Lsp1109I GCAGC 5 cut(s) 142, 164, 404, 717, 922
LweI GCATC 4 cut(s) 112, 430, 525, 751
MabI ACCWGGT 1 cut(s) 946
MaeI CTAG 4 cut(s) 429, 686, 1010, 1102
MaeII ACGT 1 cut(s) 1088
MaeIII GTNAC 3 cut(s) 410, 956, 981
MalI GATC 5 cut(s) 79, 228, 256, 267, 650
MboI GATC 5 cut(s) 77, 226, 254, 265, 648
MboII GAAGA 8 cut(s) 59, 140, 390, 476, 479, 512, 557, 770
MflI RGATCY 1 cut(s) 77
MhlI GDGCHC 2 cut(s) 528, 826
MluCI AATT 3 cut(s) 338, 774, 1053
MlyI GAGTC 2 cut(s) 32, 973
MmeI TCCRAC 3 cut(s) 165, 803, 869
MnlI CCTC 7 cut(s) 32, 406, 854, 899, 920, 972, 1064
MseI TTAA 2 cut(s) 564, 768
MslI CAYNNNNRTG 3 cut(s) 12, 137, 521
MspA1I CMGCKG 1 cut(s) 155
MspR9I CCNGG 2 cut(s) 28, 948
MvaI CCWGG 2 cut(s) 28, 948
MwoI GCNNNNNNNGC 3 cut(s) 100, 682, 881
NdeII GATC 5 cut(s) 77, 226, 254, 265, 648
NlaIII CATG 7 cut(s) 93, 136, 151, 241, 496, 589, 852
NlaIV GGNNCC 1 cut(s) 490
NmeAIII GCCGAG 1 cut(s) 847
NmuCI GTSAC 2 cut(s) 956, 981
PagI TCATGA 2 cut(s) 237, 585
PfeI GAWTC 2 cut(s) 578, 794
PflMI CCANNNNNTGG 1 cut(s) 373
PfoI TCCNGGA 1 cut(s) 26
PkrI GCNGC 6 cut(s) 154, 157, 394, 707, 924, 937
PleI GAGTC 2 cut(s) 31, 972
PmaCI CACGTG 1 cut(s) 1089
PmlI CACGTG 1 cut(s) 1089
PpsI GAGTC 2 cut(s) 31, 972
Ppu21I YACGTR 1 cut(s) 1089
PshAI GACNNNNGTC 2 cut(s) 663, 963
Psp124BI GAGCTC 1 cut(s) 826
Psp6I CCWGG 2 cut(s) 26, 946
PspCI CACGTG 1 cut(s) 1089
PspFI CCCAGC 2 cut(s) 388, 1078
PspGI CCWGG 2 cut(s) 26, 946
PspN4I GGNNCC 1 cut(s) 490
PspPI GGNCC 1 cut(s) 669
PstNI CAGNNNCTG 1 cut(s) 224
PsuI RGATCY 1 cut(s) 77
PvuII CAGCTG 1 cut(s) 155
RsaI GTAC 1 cut(s) 199
RsaNI GTAC 1 cut(s) 198
RseI CAYNNNNRTG 3 cut(s) 12, 137, 521
SacI GAGCTC 1 cut(s) 826
SaqAI TTAA 2 cut(s) 564, 768
SatI GCNGC 6 cut(s) 153, 156, 393, 706, 923, 936
Sau3AI GATC 5 cut(s) 77, 226, 254, 265, 648
Sau96I GGNCC 1 cut(s) 669
SchI GAGTC 2 cut(s) 32, 973
ScrFI CCNGG 2 cut(s) 28, 948
SduI GDGCHC 2 cut(s) 528, 826
SexAI ACCWGGT 1 cut(s) 946
SfaNI GCATC 4 cut(s) 112, 430, 525, 751
SfcI CTRYAG 1 cut(s) 817
SmiMI CAYNNNNRTG 3 cut(s) 12, 137, 521
SmlI CTYRAG 2 cut(s) 709, 1028
SmoI CTYRAG 2 cut(s) 709, 1028
Sse9I AATT 3 cut(s) 338, 774, 1053
SsiI CCGC 1 cut(s) 922
SspI AATATT 1 cut(s) 680
SspMI CTAG 4 cut(s) 429, 686, 1010, 1102
SstI GAGCTC 1 cut(s) 826
StyD4I CCNGG 2 cut(s) 26, 946
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 3 cut(s) 197, 281, 574
TaiI ACGT 1 cut(s) 1091
TaqI TCGA 4 cut(s) 313, 383, 651, 826
TasI AATT 3 cut(s) 338, 774, 1053
TauI GCSGC 1 cut(s) 925
TfiI GAWTC 2 cut(s) 578, 794
Tru1I TTAA 2 cut(s) 564, 768
Tru9I TTAA 2 cut(s) 564, 768
TscAI CASTG 3 cut(s) 442, 465, 735
TseFI GTSAC 2 cut(s) 956, 981
TseI GCWGC 5 cut(s) 152, 155, 392, 705, 935
Tsp45I GTSAC 2 cut(s) 956, 981
TspDTI ATGAA 7 cut(s) 30, 78, 226, 574, 602, 771, 1055
TspGWI ACGGA 2 cut(s) 35, 978
TspRI CASTG 3 cut(s) 442, 465, 735
Van91I CCANNNNNTGG 1 cut(s) 373
VneI GTGCAC 1 cut(s) 524
XapI RAATTY 2 cut(s) 774, 1053
XbaI TCTAGA 1 cut(s) 428
XspI CTAG 4 cut(s) 429, 686, 1010, 1102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.