RLG00000022147

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0017392
Physical Location & Seq
Reverse (-)
81314565 .. 81316376
1812 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022147

Sequence Viewer

Length: 975 bp
ATGGGACACCAAGATGAAACGGAGTCCTGGAAAGCAAGAGGCCACGAAGATAATAAAGAACCAACTGATGATGTGGGATCTCATACTTCATGGCTGGCAAAGCATCCATCTGCTTTAGATGGTTTTGAAGACATGATGATGACGAGCATGGCAGCTGCCAGGGGAAAGAAAATCGTCGTCTTGTTGGATTACGACGGTACTCTTTCCCATATCGTACCAGACCCTGATCAAGCTTTCATGACTGAAGCAATGCGATCAGCCGTAGATCAAGTTGCTAACTGTTTCCCTACTGCAATCATTTCTGGAAGGCGTCGAGATAAGGTCTATGACTTTGTGAAATTAGACAATCTCTGTTATGCTGGAAGCCACGGCTTGGATATATCGACGCCATCAGCCTCTTCAAAATATAGTAACCATAAGCATCAATCTAGAACCACTGATGGAAAAGGAAATGAATCCGATGTCTTTCGTCCAGCCGTAGATTTTTTGCCCACCATTAAAAAGATAAAGAAGGCACTGGAAGAAGAAATAAAGGGTATAGAAGGTGCCATGATAGAAGATAATACATTCTGCATCTCTGTGCACTATCGGTGCGTCAAAGAAGAGAAGGTGGACAACCTTAAAGGGACAGTTGAATCTTTCATGAAAGCTTACGATAACTTTCGCATATCGGAAGGGAAAAAGGTTATGGAAATACGACCCATGATCGACTGGGACAAAGGTCGGGCCTTGCAATATTTGCTAGACACACCCGATTTCGGAAGCTCAAGTAATGAAGTTATCCCAGTGTTTATAGGAGATGACAAAACTGATGAAGATGCTTTTAAGGCTATAAAGACAATTGGGCGAGGTATTCCCATTGTTGTTTCTTCCACTCCAAAGGAAACCGAAGCATCCTACTCGTTACGTGATCCAACGGAAGTCATGCGTTTCCTGTTACAACTTGCGAAGTGGATGGAAGAAGGCTCCGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Trehalose_PPase

Protein Analysis

325

Amino Acids

36.26

Weight (kDa)

5.3

Isoelectric Point (pI)

35.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 61 - 305 1.6e-59 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017392)

Species Orthologous Gene IDs
malus_domestica MD17G1029700.v1.1
pyrus_communis pycom17g02670
rosa_chinensis RchiOBHm_Chr2g0172991
rosa_laevigata RLG00000022147
rosa_multiflora Rmu_sc0012743.1_g000008 Rmu_sc0032348.1_g000001
rosa_roxburghii Rroxscaffold_2G00079370
rosa_rugosa Rorug02G0564300
rosa_samantha Rh2AG640900 Rh2BG654600 Rh2CG619900 Rh2DG667900
rosa_wichuraiana Rw2G052900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 545
AccB7I CCANNNNNTGG 1 cut(s) 373
AclWI GGATC 2 cut(s) 85, 905
AcuI CTGAAG 1 cut(s) 264
AcyI GRCGYC 2 cut(s) 310, 386
AfaI GTAC 2 cut(s) 199, 216
AfiI CCNNNNNNNGG 2 cut(s) 373, 758
AgsI TTSAA 3 cut(s) 128, 402, 635
AjnI CCWGG 2 cut(s) 26, 158
AluBI AGCT 4 cut(s) 155, 233, 650, 765
AluI AGCT 4 cut(s) 155, 233, 650, 765
Alw21I GWGCWC 1 cut(s) 585
Alw44I GTGCAC 1 cut(s) 581
AlwI GGATC 2 cut(s) 85, 905
AlwNI CAGNNNCTG 1 cut(s) 224
AoxI GGCC 2 cut(s) 40, 726
ApaLI GTGCAC 1 cut(s) 581
ApeKI GCWGC 2 cut(s) 152, 155
AspS9I GGNCC 1 cut(s) 726
BaeGI GKGCMC 1 cut(s) 585
BanI GGYRCC 1 cut(s) 545
BbsI GAAGAC 1 cut(s) 135
Bbv12I GWGCWC 1 cut(s) 585
BbvI GCAGC 2 cut(s) 142, 164
BccI CCATC 5 cut(s) 113, 115, 397, 434, 949
BceAI ACGGC 3 cut(s) 245, 385, 461
BcgI CGANNNNNNTGC 2 cut(s) 882, 916
BciT130I CCWGG 2 cut(s) 28, 160
BclI TGATCA 1 cut(s) 226
BfaI CTAG 2 cut(s) 429, 743
BisI GCNGC 2 cut(s) 153, 156
BlsI GCNGC 2 cut(s) 154, 157
Bme1390I CCNGG 2 cut(s) 28, 160
BmgT120I GGNCC 1 cut(s) 726
BmiI GGNNCC 2 cut(s) 547, 967
BmrFI CCNGG 2 cut(s) 28, 160
BmrI ACTGGG 2 cut(s) 721, 779
BmsI GCATC 5 cut(s) 112, 430, 582, 808, 902
BmuI ACTGGG 2 cut(s) 721, 779
BoxI GACNNNNGTC 1 cut(s) 720
BpiI GAAGAC 1 cut(s) 135
BpuEI CTTGAG 1 cut(s) 751
BsaAI YACGTR 1 cut(s) 908
BsaHI GRCGYC 2 cut(s) 310, 386
BsaJI CCNNGG 2 cut(s) 159, 367
Bsc4I CCNNNNNNNGG 2 cut(s) 373, 758
Bse1I ACTGG 3 cut(s) 522, 716, 785
Bse3DI GCAATG 1 cut(s) 255
BseBI CCWGG 2 cut(s) 28, 160
BseDI CCNNGG 2 cut(s) 159, 367
BseGI GGATG 3 cut(s) 103, 893, 960
BseLI CCNNNNNNNGG 2 cut(s) 373, 758
BseMI GCAATG 1 cut(s) 255
BseNI ACTGG 3 cut(s) 522, 716, 785
BseSI GKGCMC 1 cut(s) 585
BseXI GCAGC 2 cut(s) 142, 164
BshFI GGCC 2 cut(s) 42, 728
BshNI GGYRCC 1 cut(s) 545
BsiHKAI GWGCWC 1 cut(s) 585
BslFI GGGAC 3 cut(s) 18, 640, 728
BslI CCNNNNNNNGG 2 cut(s) 373, 758
BsmFI GGGAC 3 cut(s) 18, 640, 728
BsnI GGCC 2 cut(s) 42, 728
Bsp1286I GDGCHC 1 cut(s) 585
Bsp143I GATC 6 cut(s) 77, 226, 254, 265, 705, 910
BspANI GGCC 2 cut(s) 42, 728
BspHI TCATGA 2 cut(s) 237, 642
BspLI GGNNCC 2 cut(s) 547, 967
BspPI GGATC 2 cut(s) 85, 905
BspT107I GGYRCC 1 cut(s) 545
BsrDI GCAATG 1 cut(s) 255
BsrI ACTGG 3 cut(s) 522, 716, 785
BssECI CCNNGG 2 cut(s) 159, 367
BssMI GATC 6 cut(s) 77, 226, 254, 265, 705, 910
BssNI GRCGYC 2 cut(s) 310, 386
Bst2UI CCWGG 2 cut(s) 28, 160
Bst4CI ACNGT 3 cut(s) 197, 281, 631
Bst6I CTCTTC 2 cut(s) 403, 597
BstACI GRCGYC 2 cut(s) 310, 386
BstAPI GCANNNNNTGC 1 cut(s) 739
BstBAI YACGTR 1 cut(s) 908
BstC8I GCNNGC 1 cut(s) 96
BstDSI CCRYGG 1 cut(s) 367
BstF5I GGATG 3 cut(s) 103, 893, 960
BstKTI GATC 6 cut(s) 80, 229, 257, 268, 708, 913
BstMBI GATC 6 cut(s) 77, 226, 254, 265, 705, 910
BstMWI GCNNNNNNNGC 3 cut(s) 100, 739, 827
BstNI CCWGG 2 cut(s) 28, 160
BstPAI GACNNNNGTC 1 cut(s) 720
BstSCI CCNGG 2 cut(s) 26, 158
BstSLI GKGCMC 1 cut(s) 585
BstV1I GCAGC 2 cut(s) 142, 164
BstV2I GAAGAC 1 cut(s) 135
BstX2I RGATCY 1 cut(s) 77
BstYI RGATCY 1 cut(s) 77
BsuRI GGCC 2 cut(s) 42, 728
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 3 cut(s) 103, 893, 960
BtsIMutI CAGTG 3 cut(s) 435, 515, 792
Cac8I GCNNGC 1 cut(s) 96
CaiI CAGNNNCTG 1 cut(s) 224
CciI TCATGA 2 cut(s) 237, 642
Cfr13I GGNCC 1 cut(s) 726
CseI GACGC 3 cut(s) 299, 394, 583
Csp6I GTAC 2 cut(s) 198, 215
CviAII CATG 8 cut(s) 90, 133, 148, 238, 550, 643, 703, 925
CviQI GTAC 2 cut(s) 198, 215
DpnI GATC 6 cut(s) 79, 228, 256, 267, 707, 912
DpnII GATC 6 cut(s) 77, 226, 254, 265, 705, 910
Eam1104I CTCTTC 2 cut(s) 403, 597
EarI CTCTTC 2 cut(s) 403, 597
Eco57I CTGAAG 1 cut(s) 264
EcoRII CCWGG 2 cut(s) 26, 158
FaeI CATG 8 cut(s) 93, 136, 151, 241, 553, 646, 706, 928
FaqI GGGAC 3 cut(s) 18, 640, 728
FatI CATG 8 cut(s) 89, 132, 147, 237, 549, 642, 702, 924
FbaI TGATCA 1 cut(s) 226
Fnu4HI GCNGC 2 cut(s) 153, 156
FokI GGATG 3 cut(s) 90, 880, 967
Fsp4HI GCNGC 2 cut(s) 153, 156
FspBI CTAG 2 cut(s) 429, 743
GluI GCNGC 2 cut(s) 153, 156
HaeIII GGCC 2 cut(s) 42, 728
HgaI GACGC 3 cut(s) 299, 394, 583
Hin1I GRCGYC 2 cut(s) 310, 386
Hin1II CATG 8 cut(s) 93, 136, 151, 241, 553, 646, 706, 928
HindIII AAGCTT 2 cut(s) 231, 648
HinfI GANTC 3 cut(s) 23, 455, 635
Hpy166II GTNNAC 2 cut(s) 583, 613
Hpy188I TCNGA 3 cut(s) 460, 673, 761
Hpy188III TCNNGA 5 cut(s) 238, 303, 314, 429, 643
Hpy8I GTNNAC 2 cut(s) 583, 613
Hpy99I CGWCG 4 cut(s) 179, 197, 315, 388
HpyAV CCTTC 6 cut(s) 300, 505, 536, 601, 668, 956
HpyCH4III ACNGT 3 cut(s) 197, 281, 631
HpyCH4IV ACGT 1 cut(s) 907
HpyCH4V TGCA 4 cut(s) 293, 573, 583, 733
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 739, 827
HpySE526I ACGT 1 cut(s) 907
Hsp92I GRCGYC 2 cut(s) 310, 386
Hsp92II CATG 8 cut(s) 93, 136, 151, 241, 553, 646, 706, 928
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 6 cut(s) 77, 226, 254, 265, 705, 910
LmnI GCTCC 1 cut(s) 971
Lsp1109I GCAGC 2 cut(s) 142, 164
LweI GCATC 5 cut(s) 112, 430, 582, 808, 902
MaeI CTAG 2 cut(s) 429, 743
MaeII ACGT 1 cut(s) 907
MaeIII GTNAC 3 cut(s) 410, 903, 936
MalI GATC 6 cut(s) 79, 228, 256, 267, 707, 912
MboI GATC 6 cut(s) 77, 226, 254, 265, 705, 910
MfeI CAATTG 1 cut(s) 840
MflI RGATCY 1 cut(s) 77
MhlI GDGCHC 1 cut(s) 585
MluCI AATT 2 cut(s) 338, 840
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 2 cut(s) 165, 938
MnlI CCTC 3 cut(s) 32, 406, 842
MseI TTAA 3 cut(s) 498, 621, 825
MslI CAYNNNNRTG 3 cut(s) 12, 137, 578
MspA1I CMGCKG 1 cut(s) 155
MspR9I CCNGG 2 cut(s) 28, 160
MunI CAATTG 1 cut(s) 840
MvaI CCWGG 2 cut(s) 28, 160
MwoI GCNNNNNNNGC 3 cut(s) 100, 739, 827
NdeII GATC 6 cut(s) 77, 226, 254, 265, 705, 910
NlaIII CATG 8 cut(s) 93, 136, 151, 241, 553, 646, 706, 928
NlaIV GGNNCC 2 cut(s) 547, 967
PagI TCATGA 2 cut(s) 237, 642
PfeI GAWTC 2 cut(s) 455, 635
PflMI CCANNNNNTGG 1 cut(s) 373
PfoI TCCNGGA 1 cut(s) 26
PkrI GCNGC 2 cut(s) 154, 157
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
Ppu21I YACGTR 1 cut(s) 908
PshAI GACNNNNGTC 1 cut(s) 720
Psp6I CCWGG 2 cut(s) 26, 158
PspGI CCWGG 2 cut(s) 26, 158
PspN4I GGNNCC 2 cut(s) 547, 967
PspPI GGNCC 1 cut(s) 726
PstNI CAGNNNCTG 1 cut(s) 224
PsuI RGATCY 1 cut(s) 77
PvuII CAGCTG 1 cut(s) 155
RsaI GTAC 2 cut(s) 199, 216
RsaNI GTAC 2 cut(s) 198, 215
RseI CAYNNNNRTG 3 cut(s) 12, 137, 578
SaqAI TTAA 3 cut(s) 498, 621, 825
SatI GCNGC 2 cut(s) 153, 156
Sau3AI GATC 6 cut(s) 77, 226, 254, 265, 705, 910
Sau96I GGNCC 1 cut(s) 726
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 2 cut(s) 28, 160
SduI GDGCHC 1 cut(s) 585
SfaNI GCATC 5 cut(s) 112, 430, 582, 808, 902
SmiMI CAYNNNNRTG 3 cut(s) 12, 137, 578
SmlI CTYRAG 1 cut(s) 766
SmoI CTYRAG 1 cut(s) 766
Sse9I AATT 2 cut(s) 338, 840
SspI AATATT 1 cut(s) 737
SspMI CTAG 2 cut(s) 429, 743
StyD4I CCNGG 2 cut(s) 26, 158
TaaI ACNGT 3 cut(s) 197, 281, 631
TaiI ACGT 1 cut(s) 910
TaqI TCGA 3 cut(s) 313, 383, 708
TasI AATT 2 cut(s) 338, 840
TfiI GAWTC 2 cut(s) 455, 635
Tru1I TTAA 3 cut(s) 498, 621, 825
Tru9I TTAA 3 cut(s) 498, 621, 825
TscAI CASTG 3 cut(s) 442, 522, 792
TseI GCWGC 2 cut(s) 152, 155
TspDTI ATGAA 8 cut(s) 30, 78, 226, 468, 631, 659, 789, 828
TspGWI ACGGA 3 cut(s) 35, 932, 958
TspRI CASTG 3 cut(s) 442, 522, 792
Van91I CCANNNNNTGG 1 cut(s) 373
VneI GTGCAC 1 cut(s) 581
XbaI TCTAGA 1 cut(s) 428
XspI CTAG 2 cut(s) 429, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.