Rroxscaffold_2G00079370

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0017392
Physical Location & Seq
Forward (+)
2405350 .. 2407152
1803 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00079370.1

Sequence Viewer

Length: 918 bp
ATGGGACACCAAGATGAAACGGAGTCCTGGAAAGCAAGAGGCCACAAAGATAATAGAGAACCAACTGATGATGTGGGATCTCATACTTCATGGCTGGAAAAGCATCCATCTGCTTTAGATGGTTTTGAAGACATGATGATGACGAGCATGGCAGCTGCCAAGGGAAAGAAAATCGTCGTCTTGTTGGATTACGACGGTACTCTTTCCCATATCGTACCAGACCCTGATCAAGCTTTCATGACTGAAGCAATGCGATCAGCCGTAGATCAAGTTGCTAACTGTTTCCCTACTGCAATCATTTCTGGAAGGCGTCGAGATAAGGTCTATGACTTTGTGAAATTAGACAATCTCTGTTATGCTGGAAGCCACGGCTTGGATATATCGACCCCATCAGCCTCTTCAAAATATAGTAACCATAAGCATCAATCTAGAACCACTGATGGAAAAATAAAGAAGGCACTGGAAGAAGAAATAAAGGGTATAGAAGGTGCCATGATAGAAGATAATACATTCTGCATCTCTGTGCACTATCGGTGCGTCAAAGAAGAGAAGGTGGACAACCTTAAAGGGACAGTTGAATCTTTCATGAAATCTTACGATAACTTTCGCATATCGGAAGGGAAAAAGGTTATGGAAATACGACCTAAGATCGACTGGGACAAAGGTCGGGCCTTGCAATATTTGCTAGACACACCCGATTTCGGCAGCTCAAGTAATGAAGTTATCCCAGTGTTTATAGGAGATGACAAAACTGATGAAGATGCTTTTAAGGCTATAAAGTCAATTGGCCGAGGTATTCCCATTGTTGTTTCTTCCACTCCAAAGGAGACCGAAGCATCCTACTCGTTACGTGATCCAACGGAAGTCATGCGTTTCCTGTTACAACTTGCGAAGTGGATGGAAGAAGGCTCCGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Trehalose_PPase

Protein Analysis

305

Amino Acids

34.2

Weight (kDa)

5.46

Isoelectric Point (pI)

35.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 61 - 286 3.3e-59 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017392)

Species Orthologous Gene IDs
malus_domestica MD17G1029700.v1.1
pyrus_communis pycom17g02670
rosa_chinensis RchiOBHm_Chr2g0172991
rosa_laevigata RLG00000022147
rosa_multiflora Rmu_sc0012743.1_g000008 Rmu_sc0032348.1_g000001
rosa_roxburghii Rroxscaffold_2G00079370
rosa_rugosa Rorug02G0564300
rosa_samantha Rh2AG640900 Rh2BG654600 Rh2CG619900 Rh2DG667900
rosa_wichuraiana Rw2G052900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 488
AccB7I CCANNNNNTGG 1 cut(s) 373
AclWI GGATC 2 cut(s) 85, 848
AcoI YGGCCR 1 cut(s) 787
AcuI CTGAAG 1 cut(s) 264
AcyI GRCGYC 1 cut(s) 310
AfaI GTAC 2 cut(s) 199, 216
AfiI CCNNNNNNNGG 2 cut(s) 373, 701
AgsI TTSAA 3 cut(s) 128, 402, 578
AjnI CCWGG 1 cut(s) 26
AluBI AGCT 3 cut(s) 155, 233, 708
AluI AGCT 3 cut(s) 155, 233, 708
Alw21I GWGCWC 1 cut(s) 528
Alw26I GTCTC 1 cut(s) 821
Alw44I GTGCAC 1 cut(s) 524
AlwI GGATC 2 cut(s) 85, 848
AlwNI CAGNNNCTG 1 cut(s) 224
AoxI GGCC 3 cut(s) 40, 669, 787
ApaLI GTGCAC 1 cut(s) 524
ApeKI GCWGC 3 cut(s) 152, 155, 705
AspS9I GGNCC 1 cut(s) 669
BaeGI GKGCMC 1 cut(s) 528
BanI GGYRCC 1 cut(s) 488
BbsI GAAGAC 1 cut(s) 135
Bbv12I GWGCWC 1 cut(s) 528
BbvI GCAGC 3 cut(s) 142, 164, 717
BccI CCATC 5 cut(s) 113, 115, 397, 434, 892
BceAI ACGGC 2 cut(s) 245, 385
BcgI CGANNNNNNTGC 2 cut(s) 825, 859
BciT130I CCWGG 1 cut(s) 28
BclI TGATCA 1 cut(s) 226
BcoDI GTCTC 1 cut(s) 821
BfaI CTAG 2 cut(s) 429, 686
BisI GCNGC 3 cut(s) 153, 156, 706
BlsI GCNGC 3 cut(s) 154, 157, 707
Bme1390I CCNGG 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 669
BmiI GGNNCC 2 cut(s) 490, 910
BmrFI CCNGG 1 cut(s) 28
BmrI ACTGGG 2 cut(s) 664, 722
BmsI GCATC 5 cut(s) 112, 430, 525, 751, 845
BmuI ACTGGG 2 cut(s) 664, 722
BoxI GACNNNNGTC 1 cut(s) 663
BpiI GAAGAC 1 cut(s) 135
BpuEI CTTGAG 1 cut(s) 694
BsaAI YACGTR 1 cut(s) 851
BsaHI GRCGYC 1 cut(s) 310
BsaI GGTCTC 1 cut(s) 821
BsaJI CCNNGG 3 cut(s) 159, 367, 790
Bsc4I CCNNNNNNNGG 2 cut(s) 373, 701
Bse1I ACTGG 3 cut(s) 465, 659, 728
Bse3DI GCAATG 1 cut(s) 255
BseBI CCWGG 1 cut(s) 28
BseDI CCNNGG 3 cut(s) 159, 367, 790
BseGI GGATG 3 cut(s) 103, 836, 903
BseLI CCNNNNNNNGG 2 cut(s) 373, 701
BseMI GCAATG 1 cut(s) 255
BseNI ACTGG 3 cut(s) 465, 659, 728
BseSI GKGCMC 1 cut(s) 528
BseXI GCAGC 3 cut(s) 142, 164, 717
BshFI GGCC 3 cut(s) 42, 671, 789
BshNI GGYRCC 1 cut(s) 488
BsiHKAI GWGCWC 1 cut(s) 528
BslFI GGGAC 3 cut(s) 18, 583, 671
BslI CCNNNNNNNGG 2 cut(s) 373, 701
BsmAI GTCTC 1 cut(s) 821
BsmFI GGGAC 3 cut(s) 18, 583, 671
BsnI GGCC 3 cut(s) 42, 671, 789
Bso31I GGTCTC 1 cut(s) 821
Bsp1286I GDGCHC 1 cut(s) 528
Bsp143I GATC 6 cut(s) 77, 226, 254, 265, 648, 853
BspANI GGCC 3 cut(s) 42, 671, 789
BspHI TCATGA 2 cut(s) 237, 585
BspLI GGNNCC 2 cut(s) 490, 910
BspPI GGATC 2 cut(s) 85, 848
BspT107I GGYRCC 1 cut(s) 488
BspTNI GGTCTC 1 cut(s) 821
BsrDI GCAATG 1 cut(s) 255
BsrI ACTGG 3 cut(s) 465, 659, 728
BssECI CCNNGG 3 cut(s) 159, 367, 790
BssMI GATC 6 cut(s) 77, 226, 254, 265, 648, 853
BssNI GRCGYC 1 cut(s) 310
BssT1I CCWWGG 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 28
Bst4CI ACNGT 3 cut(s) 197, 281, 574
Bst6I CTCTTC 2 cut(s) 403, 540
BstACI GRCGYC 1 cut(s) 310
BstAPI GCANNNNNTGC 1 cut(s) 682
BstBAI YACGTR 1 cut(s) 851
BstDEI CTNAG 1 cut(s) 645
BstDSI CCRYGG 1 cut(s) 367
BstF5I GGATG 3 cut(s) 103, 836, 903
BstKTI GATC 6 cut(s) 80, 229, 257, 268, 651, 856
BstMAI GTCTC 1 cut(s) 821
BstMBI GATC 6 cut(s) 77, 226, 254, 265, 648, 853
BstMWI GCNNNNNNNGC 3 cut(s) 100, 682, 770
BstNI CCWGG 1 cut(s) 28
BstPAI GACNNNNGTC 1 cut(s) 663
BstSCI CCNGG 1 cut(s) 26
BstSLI GKGCMC 1 cut(s) 528
BstV1I GCAGC 3 cut(s) 142, 164, 717
BstV2I GAAGAC 1 cut(s) 135
BstX2I RGATCY 1 cut(s) 77
BstYI RGATCY 1 cut(s) 77
BsuRI GGCC 3 cut(s) 42, 671, 789
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 3 cut(s) 103, 836, 903
BtsIMutI CAGTG 3 cut(s) 435, 458, 735
CaiI CAGNNNCTG 1 cut(s) 224
CciI TCATGA 2 cut(s) 237, 585
Cfr13I GGNCC 1 cut(s) 669
CseI GACGC 2 cut(s) 299, 526
Csp6I GTAC 2 cut(s) 198, 215
CviAII CATG 7 cut(s) 90, 133, 148, 238, 493, 586, 868
CviQI GTAC 2 cut(s) 198, 215
DdeI CTNAG 1 cut(s) 645
DpnI GATC 6 cut(s) 79, 228, 256, 267, 650, 855
DpnII GATC 6 cut(s) 77, 226, 254, 265, 648, 853
EaeI YGGCCR 1 cut(s) 787
Eam1104I CTCTTC 2 cut(s) 403, 540
EarI CTCTTC 2 cut(s) 403, 540
Eco130I CCWWGG 1 cut(s) 159
Eco31I GGTCTC 1 cut(s) 821
Eco57I CTGAAG 1 cut(s) 264
EcoRII CCWGG 1 cut(s) 26
EcoT14I CCWWGG 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 7 cut(s) 93, 136, 151, 241, 496, 589, 871
FaqI GGGAC 3 cut(s) 18, 583, 671
FatI CATG 7 cut(s) 89, 132, 147, 237, 492, 585, 867
FbaI TGATCA 1 cut(s) 226
Fnu4HI GCNGC 3 cut(s) 153, 156, 706
FokI GGATG 3 cut(s) 90, 823, 910
Fsp4HI GCNGC 3 cut(s) 153, 156, 706
FspBI CTAG 2 cut(s) 429, 686
GluI GCNGC 3 cut(s) 153, 156, 706
HaeIII GGCC 3 cut(s) 42, 671, 789
HgaI GACGC 2 cut(s) 299, 526
Hin1I GRCGYC 1 cut(s) 310
Hin1II CATG 7 cut(s) 93, 136, 151, 241, 496, 589, 871
HindIII AAGCTT 1 cut(s) 231
HinfI GANTC 2 cut(s) 23, 578
Hpy166II GTNNAC 2 cut(s) 526, 556
Hpy188I TCNGA 1 cut(s) 616
Hpy188III TCNNGA 5 cut(s) 238, 303, 314, 429, 586
Hpy8I GTNNAC 2 cut(s) 526, 556
Hpy99I CGWCG 3 cut(s) 179, 197, 315
HpyAV CCTTC 6 cut(s) 300, 448, 479, 544, 611, 899
HpyCH4III ACNGT 3 cut(s) 197, 281, 574
HpyCH4IV ACGT 1 cut(s) 850
HpyCH4V TGCA 4 cut(s) 293, 516, 526, 676
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 682, 770
HpyF3I CTNAG 1 cut(s) 645
HpySE526I ACGT 1 cut(s) 850
Hsp92I GRCGYC 1 cut(s) 310
Hsp92II CATG 7 cut(s) 93, 136, 151, 241, 496, 589, 871
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 6 cut(s) 77, 226, 254, 265, 648, 853
LmnI GCTCC 1 cut(s) 914
Lsp1109I GCAGC 3 cut(s) 142, 164, 717
LweI GCATC 5 cut(s) 112, 430, 525, 751, 845
MaeI CTAG 2 cut(s) 429, 686
MaeII ACGT 1 cut(s) 850
MaeIII GTNAC 3 cut(s) 410, 846, 879
MalI GATC 6 cut(s) 79, 228, 256, 267, 650, 855
MboI GATC 6 cut(s) 77, 226, 254, 265, 648, 853
MboII GAAGA 9 cut(s) 140, 390, 476, 479, 512, 557, 770, 804, 914
MfeI CAATTG 1 cut(s) 783
MflI RGATCY 1 cut(s) 77
MhlI GDGCHC 1 cut(s) 528
MluCI AATT 2 cut(s) 338, 783
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 2 cut(s) 165, 881
MnlI CCTC 3 cut(s) 32, 406, 785
MseI TTAA 2 cut(s) 564, 768
MslI CAYNNNNRTG 3 cut(s) 12, 137, 521
MspA1I CMGCKG 1 cut(s) 155
MspR9I CCNGG 1 cut(s) 28
MunI CAATTG 1 cut(s) 783
MvaI CCWGG 1 cut(s) 28
MwoI GCNNNNNNNGC 3 cut(s) 100, 682, 770
NdeII GATC 6 cut(s) 77, 226, 254, 265, 648, 853
NlaIII CATG 7 cut(s) 93, 136, 151, 241, 496, 589, 871
NlaIV GGNNCC 2 cut(s) 490, 910
NmeAIII GCCGAG 1 cut(s) 815
PagI TCATGA 2 cut(s) 237, 585
PfeI GAWTC 1 cut(s) 578
PflMI CCANNNNNTGG 1 cut(s) 373
PfoI TCCNGGA 1 cut(s) 26
PkrI GCNGC 3 cut(s) 154, 157, 707
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
Ppu21I YACGTR 1 cut(s) 851
PshAI GACNNNNGTC 1 cut(s) 663
Psp6I CCWGG 1 cut(s) 26
PspGI CCWGG 1 cut(s) 26
PspN4I GGNNCC 2 cut(s) 490, 910
PspPI GGNCC 1 cut(s) 669
PstNI CAGNNNCTG 1 cut(s) 224
PsuI RGATCY 1 cut(s) 77
PvuII CAGCTG 1 cut(s) 155
RsaI GTAC 2 cut(s) 199, 216
RsaNI GTAC 2 cut(s) 198, 215
RseI CAYNNNNRTG 3 cut(s) 12, 137, 521
SaqAI TTAA 2 cut(s) 564, 768
SatI GCNGC 3 cut(s) 153, 156, 706
Sau3AI GATC 6 cut(s) 77, 226, 254, 265, 648, 853
Sau96I GGNCC 1 cut(s) 669
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 1 cut(s) 28
SduI GDGCHC 1 cut(s) 528
SfaNI GCATC 5 cut(s) 112, 430, 525, 751, 845
SmiMI CAYNNNNRTG 3 cut(s) 12, 137, 521
SmlI CTYRAG 1 cut(s) 709
SmoI CTYRAG 1 cut(s) 709
Sse9I AATT 2 cut(s) 338, 783
SspI AATATT 1 cut(s) 680
SspMI CTAG 2 cut(s) 429, 686
StyD4I CCNGG 1 cut(s) 26
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 3 cut(s) 197, 281, 574
TaiI ACGT 1 cut(s) 853
TaqI TCGA 3 cut(s) 313, 383, 651
TaqII GACCGA 1 cut(s) 845
TasI AATT 2 cut(s) 338, 783
TfiI GAWTC 1 cut(s) 578
Tru1I TTAA 2 cut(s) 564, 768
Tru9I TTAA 2 cut(s) 564, 768
TscAI CASTG 3 cut(s) 442, 465, 735
TseI GCWGC 3 cut(s) 152, 155, 705
TspDTI ATGAA 7 cut(s) 30, 78, 226, 574, 602, 732, 771
TspGWI ACGGA 3 cut(s) 35, 875, 901
TspRI CASTG 3 cut(s) 442, 465, 735
Van91I CCANNNNNTGG 1 cut(s) 373
VneI GTGCAC 1 cut(s) 524
XbaI TCTAGA 1 cut(s) 428
XspI CTAG 2 cut(s) 429, 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.