MD17G1033100.v1.1

Universal stress protein A-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014139
Physical Location & Seq
Reverse (-)
2376958 .. 2381067
4110 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1033100.v1.1.491

Sequence Viewer

Length: 705 bp
ATGCCCTATCCTACGCCTAGATGCGCGATGTGCTTAATCGGGCCTACGGCTGCGAACCCACAAAACGAAGCAAGTCATCCTTCGGTTACCGCCTTCATCAGAGAGAGAGAGAGTACTGTGGTTCCAGAAAGTTCGAATCAGCAAGCGAGTCGACTCAGTGTCGTGAGCGAAGCGAAGATGGCAAGCGAGCCAACTCGGATAATGCTGGCAGTGAACGAGTCGACCATCAAGGGCTACCCGCACGCCTCCATCAGCAGCAGGAAGGCGTTCGAGTGGACGCTCCAGAAGATCGTGCGCTCCAACACCAGTGGTTTCAAGCTCCAGTTCCTGCACGTCCAAGTCCCCGATGAAGACGGTTTTGATGACATGGACAGTGTCTATGCATCGCCGGAGGATTTTAAAAACATGAAGGACAGAGACCGCGCCAGAGGGCTTCATCTGCTGGAGTTCTTCGTTGACAGATGTAATGCAATTGGGGTTGCTTGTGAAGCATGGATCAAAAGAGGGGATGCCAAGGAAGTAATCTGCCGTGAGGTGAAGCGTGTCCGGCCAGACTTTCTGGTGGTGGGCTGTCGGGGGCTTGGACCTTTCCAGAGGGTTTTTGTTGGGACTGTGAGCGAGTTCTGCGTGAAGCACGCTGAGTGCCCTGTCATCACAATCAAGCGCAGCGCAGAGGAAACACCCCAGGATCCCGTCGATGACTGA

Protein Analysis

235

Amino Acids

26.12

Weight (kDa)

6.24

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 83 - 221 3.7e-17 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014139)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01520 AT5G14680
fragaria_vesca FvH4_6g50880
malus_domestica MD09G1031200.v1.1 MD17G1033100.v1.1
prunus_persica Prupe.3G286400_v2.0.a1
pyrus_communis pycom17g03050
rosa_chinensis RchiOBHm_Chr2g0172261
rosa_laevigata RLG00000022099
rosa_multiflora Rmu_ssc0000103.1_g000006
rosa_roxburghii Rroxscaffold_2G00080020
rosa_rugosa Rorug02G0558200
rosa_samantha Rh2AG633200 Rh2BG646800 Rh2CG613800 Rh2DG661700
rosa_wichuraiana Rw2G052430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 151, 221
AccII CGCG 2 cut(s) 26, 423
AciI CCGC 3 cut(s) 90, 239, 421
AclWI GGATC 3 cut(s) 503, 683, 696
AcoI YGGCCR 1 cut(s) 548
AfaI GTAC 1 cut(s) 115
AgsI TTSAA 1 cut(s) 316
AhdI GACNNNNNGTC 1 cut(s) 158
AjiI CACGTC 1 cut(s) 334
AjnI CCWGG 1 cut(s) 684
AluBI AGCT 1 cut(s) 319
AluI AGCT 1 cut(s) 319
Alw26I GTCTC 1 cut(s) 411
AlwI GGATC 3 cut(s) 503, 683, 696
AlwNI CAGNNNCTG 1 cut(s) 328
AoxI GGCC 2 cut(s) 41, 548
ApeKI GCWGC 3 cut(s) 50, 255, 666
Asp700I GAANNNNTTC 1 cut(s) 266
AspLEI GCGC 5 cut(s) 26, 297, 425, 666, 671
AspS9I GGNCC 2 cut(s) 41, 584
AsuHPI GGTGA 1 cut(s) 547
AsuII TTCGAA 1 cut(s) 134
AvaII GGWCC 1 cut(s) 584
BaeGI GKGCMC 1 cut(s) 647
BamHI GGATCC 1 cut(s) 688
BbsI GAAGAC 1 cut(s) 357
BbvI GCAGC 3 cut(s) 37, 267, 678
BccI CCATC 3 cut(s) 172, 233, 257
BceAI ACGGC 2 cut(s) 63, 513
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BciT130I CCWGG 1 cut(s) 686
BcoDI GTCTC 1 cut(s) 411
BfaI CTAG 1 cut(s) 18
BisI GCNGC 3 cut(s) 51, 256, 667
BlsI GCNGC 3 cut(s) 52, 257, 668
BmcAI AGTACT 1 cut(s) 115
Bme1390I CCNGG 1 cut(s) 686
Bme18I GGWCC 1 cut(s) 584
BmeRI GACNNNNNGTC 1 cut(s) 158
BmgBI CACGTC 1 cut(s) 334
BmgT120I GGNCC 2 cut(s) 41, 584
BmiI GGNNCC 2 cut(s) 123, 690
BmrFI CCNGG 1 cut(s) 686
BmsI GCATC 3 cut(s) 11, 392, 499
BpiI GAAGAC 1 cut(s) 357
BpmI CTGGAG 3 cut(s) 266, 305, 464
Bpu14I TTCGAA 1 cut(s) 134
BsaI GGTCTC 1 cut(s) 411
BsaJI CCNNGG 2 cut(s) 513, 684
Bse1I ACTGG 2 cut(s) 306, 322
BseBI CCWGG 1 cut(s) 686
BseDI CCNNGG 2 cut(s) 513, 684
BseGI GGATG 2 cut(s) 76, 514
BseMII CTCAG 2 cut(s) 169, 630
BseNI ACTGG 2 cut(s) 306, 322
BseSI GKGCMC 1 cut(s) 647
BseXI GCAGC 3 cut(s) 37, 267, 678
BsgI GTGCAG 1 cut(s) 314
Bsh1236I CGCG 2 cut(s) 26, 423
BshFI GGCC 2 cut(s) 43, 550
BsiSI CCGG 2 cut(s) 389, 547
BslFI GGGAC 2 cut(s) 326, 622
BsmAI GTCTC 1 cut(s) 411
BsmFI GGGAC 2 cut(s) 326, 622
BsnI GGCC 2 cut(s) 43, 550
Bso31I GGTCTC 1 cut(s) 411
Bsp119I TTCGAA 1 cut(s) 134
Bsp1286I GDGCHC 1 cut(s) 647
Bsp143I GATC 3 cut(s) 288, 495, 688
BspACI CCGC 3 cut(s) 90, 239, 421
BspANI GGCC 2 cut(s) 43, 550
BspCNI CTCAG 2 cut(s) 168, 631
BspFNI CGCG 2 cut(s) 26, 423
BspLI GGNNCC 2 cut(s) 123, 690
BspPI GGATC 3 cut(s) 503, 683, 696
BspT104I TTCGAA 1 cut(s) 134
BspTNI GGTCTC 1 cut(s) 411
BsrI ACTGG 2 cut(s) 306, 322
BssECI CCNNGG 2 cut(s) 513, 684
BssMI GATC 3 cut(s) 288, 495, 688
BssT1I CCWWGG 1 cut(s) 513
Bst2UI CCWGG 1 cut(s) 686
Bst4CI ACNGT 4 cut(s) 118, 356, 374, 613
BstBI TTCGAA 1 cut(s) 134
BstC8I GCNNGC 6 cut(s) 144, 184, 188, 207, 243, 636
BstDEI CTNAG 2 cut(s) 155, 639
BstEII GGTNACC 1 cut(s) 85
BstF5I GGATG 2 cut(s) 76, 514
BstFNI CGCG 2 cut(s) 26, 423
BstHHI GCGC 5 cut(s) 26, 297, 425, 666, 671
BstKTI GATC 3 cut(s) 291, 498, 691
BstMAI GTCTC 1 cut(s) 411
BstMBI GATC 3 cut(s) 288, 495, 688
BstMWI GCNNNNNNNGC 6 cut(s) 30, 179, 439, 488, 547, 624
BstNI CCWGG 1 cut(s) 686
BstPI GGTNACC 1 cut(s) 85
BstSCI CCNGG 1 cut(s) 684
BstSLI GKGCMC 1 cut(s) 647
BstUI CGCG 2 cut(s) 26, 423
BstV1I GCAGC 3 cut(s) 37, 267, 678
BstV2I GAAGAC 1 cut(s) 357
BstX2I RGATCY 1 cut(s) 688
BstYI RGATCY 1 cut(s) 688
BsuRI GGCC 2 cut(s) 43, 550
BtgZI GCGATG 2 cut(s) 41, 369
BtrI CACGTC 1 cut(s) 334
BtsCI GGATG 2 cut(s) 76, 514
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 4 cut(s) 163, 216, 313, 379
Cac8I GCNNGC 6 cut(s) 144, 184, 188, 207, 243, 636
CaiI CAGNNNCTG 1 cut(s) 328
CfoI GCGC 5 cut(s) 26, 297, 425, 666, 671
Cfr13I GGNCC 2 cut(s) 41, 584
CseI GACGC 1 cut(s) 286
Csp6I GTAC 1 cut(s) 114
CspCI CAANNNNNGTGG 2 cut(s) 289, 324
CviAII CATG 3 cut(s) 367, 406, 492
CviJI RGCY 9 cut(s) 43, 50, 190, 234, 319, 433, 550, 570, 580
CviKI_1 RGCY 9 cut(s) 43, 50, 190, 234, 319, 433, 550, 570, 580
CviQI GTAC 1 cut(s) 114
DdeI CTNAG 2 cut(s) 155, 639
DpnI GATC 3 cut(s) 290, 497, 690
DpnII GATC 3 cut(s) 288, 495, 688
DraI TTTAAA 1 cut(s) 400
DriI GACNNNNNGTC 1 cut(s) 158
EaeI YGGCCR 1 cut(s) 548
Eam1105I GACNNNNNGTC 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 513
Eco31I GGTCTC 1 cut(s) 411
Eco47I GGWCC 1 cut(s) 584
Eco91I GGTNACC 1 cut(s) 85
EcoO65I GGTNACC 1 cut(s) 85
EcoRII CCWGG 1 cut(s) 684
EcoT14I CCWWGG 1 cut(s) 513
EcoT22I ATGCAT 1 cut(s) 385
ErhI CCWWGG 1 cut(s) 513
FaeI CATG 3 cut(s) 370, 409, 495
FaiI YATR 4 cut(s) 368, 381, 407, 493
FalI AAGNNNNNCTT 2 cut(s) 64, 96
FaqI GGGAC 2 cut(s) 326, 622
FatI CATG 3 cut(s) 366, 405, 491
FauI CCCGC 1 cut(s) 246
FblI GTMKAC 2 cut(s) 151, 221
Fnu4HI GCNGC 3 cut(s) 51, 256, 667
FokI GGATG 2 cut(s) 63, 521
Fsp4HI GCNGC 3 cut(s) 51, 256, 667
FspBI CTAG 1 cut(s) 18
GlaI GCGC 5 cut(s) 25, 296, 424, 665, 670
GluI GCNGC 3 cut(s) 51, 256, 667
GsuI CTGGAG 3 cut(s) 266, 305, 464
HaeIII GGCC 2 cut(s) 43, 550
HapII CCGG 2 cut(s) 389, 547
HgaI GACGC 1 cut(s) 286
HhaI GCGC 5 cut(s) 26, 297, 425, 666, 671
Hin1II CATG 3 cut(s) 370, 409, 495
Hin6I GCGC 5 cut(s) 24, 295, 423, 664, 669
HinP1I GCGC 5 cut(s) 24, 295, 423, 664, 669
HincII GTYRAC 3 cut(s) 152, 222, 457
HindII GTYRAC 3 cut(s) 152, 222, 457
HinfI GANTC 4 cut(s) 136, 148, 153, 218
HpaII CCGG 2 cut(s) 389, 547
HphI GGTGA 1 cut(s) 547
Hpy166II GTNNAC 5 cut(s) 152, 214, 222, 276, 457
Hpy188I TCNGA 2 cut(s) 101, 198
Hpy188III TCNNGA 4 cut(s) 125, 163, 283, 592
Hpy8I GTNNAC 5 cut(s) 152, 214, 222, 276, 457
Hpy99I CGWCG 1 cut(s) 698
HpyAV CCTTC 4 cut(s) 90, 103, 256, 403
HpyCH4III ACNGT 4 cut(s) 118, 356, 374, 613
HpyCH4IV ACGT 1 cut(s) 333
HpyCH4V TGCA 3 cut(s) 331, 383, 470
HpyF10VI GCNNNNNNNGC 6 cut(s) 30, 179, 439, 488, 547, 624
HpyF3I CTNAG 2 cut(s) 155, 639
HpySE526I ACGT 1 cut(s) 333
Hsp92II CATG 3 cut(s) 370, 409, 495
HspAI GCGC 5 cut(s) 24, 295, 423, 664, 669
Kzo9I GATC 3 cut(s) 288, 495, 688
LmnI GCTCC 3 cut(s) 285, 302, 324
Lsp1109I GCAGC 3 cut(s) 37, 267, 678
LweI GCATC 3 cut(s) 11, 392, 499
MaeI CTAG 1 cut(s) 18
MaeII ACGT 1 cut(s) 333
MaeIII GTNAC 1 cut(s) 85
MalI GATC 3 cut(s) 290, 497, 690
MboI GATC 3 cut(s) 288, 495, 688
MboII GAAGA 4 cut(s) 187, 298, 362, 442
MfeI CAATTG 1 cut(s) 471
MflI RGATCY 1 cut(s) 688
MhlI GDGCHC 1 cut(s) 647
MluCI AATT 1 cut(s) 471
MlyI GAGTC 3 cut(s) 147, 157, 227
MmeI TCCRAC 1 cut(s) 324
MnlI CCTC 7 cut(s) 256, 385, 422, 497, 526, 588, 667
Mph1103I ATGCAT 1 cut(s) 385
MroXI GAANNNNTTC 1 cut(s) 266
MseI TTAA 2 cut(s) 35, 399
MspI CCGG 2 cut(s) 389, 547
MspR9I CCNGG 1 cut(s) 686
MteI GCGCNGCGC 1 cut(s) 667
MunI CAATTG 1 cut(s) 471
MvaI CCWGG 1 cut(s) 686
MvnI CGCG 2 cut(s) 26, 423
MwoI GCNNNNNNNGC 6 cut(s) 30, 179, 439, 488, 547, 624
NdeII GATC 3 cut(s) 288, 495, 688
NlaIII CATG 3 cut(s) 370, 409, 495
NlaIV GGNNCC 2 cut(s) 123, 690
NsiI ATGCAT 1 cut(s) 385
NspV TTCGAA 1 cut(s) 134
PdmI GAANNNNTTC 1 cut(s) 266
PfeI GAWTC 1 cut(s) 136
PflFI GACNNNGTC 1 cut(s) 374
PkrI GCNGC 3 cut(s) 52, 257, 668
PleI GAGTC 3 cut(s) 147, 156, 226
PpsI GAGTC 3 cut(s) 147, 156, 226
Psp6I CCWGG 1 cut(s) 684
PspEI GGTNACC 1 cut(s) 85
PspGI CCWGG 1 cut(s) 684
PspN4I GGNNCC 2 cut(s) 123, 690
PspPI GGNCC 2 cut(s) 41, 584
PstNI CAGNNNCTG 1 cut(s) 328
PsuI RGATCY 1 cut(s) 688
PsyI GACNNNGTC 1 cut(s) 374
RsaI GTAC 1 cut(s) 115
RsaNI GTAC 1 cut(s) 114
SalI GTCGAC 2 cut(s) 150, 220
SaqAI TTAA 2 cut(s) 35, 399
SatI GCNGC 3 cut(s) 51, 256, 667
Sau3AI GATC 3 cut(s) 288, 495, 688
Sau96I GGNCC 2 cut(s) 41, 584
ScaI AGTACT 1 cut(s) 115
SchI GAGTC 3 cut(s) 147, 157, 227
ScrFI CCNGG 1 cut(s) 686
SduI GDGCHC 1 cut(s) 647
SetI ASST 4 cut(s) 321, 336, 537, 589
SfaNI GCATC 3 cut(s) 11, 392, 499
SfuI TTCGAA 1 cut(s) 134
SinI GGWCC 1 cut(s) 584
Sse9I AATT 1 cut(s) 471
SsiI CCGC 3 cut(s) 90, 239, 421
SspMI CTAG 1 cut(s) 18
StyD4I CCNGG 1 cut(s) 684
StyI CCWWGG 1 cut(s) 513
TaaI ACNGT 4 cut(s) 118, 356, 374, 613
TaiI ACGT 1 cut(s) 336
TaqI TCGA 5 cut(s) 134, 151, 221, 270, 696
TasI AATT 1 cut(s) 471
TatI WGTACW 1 cut(s) 113
TfiI GAWTC 1 cut(s) 136
Tru1I TTAA 2 cut(s) 35, 399
Tru9I TTAA 2 cut(s) 35, 399
TscAI CASTG 4 cut(s) 163, 216, 313, 379
TseI GCWGC 3 cut(s) 50, 255, 666
TspDTI ATGAA 4 cut(s) 85, 363, 422, 425
TspRI CASTG 4 cut(s) 163, 216, 313, 379
Tth111I GACNNNGTC 1 cut(s) 374
VpaK11BI GGWCC 1 cut(s) 584
XmiI GTMKAC 2 cut(s) 151, 221
XmnI GAANNNNTTC 1 cut(s) 266
XspI CTAG 1 cut(s) 18
ZrmI AGTACT 1 cut(s) 115
Zsp2I ATGCAT 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.