Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0007157

Physical Location & Seq

Genomic Coordinates Reverse (-)

2954781 .. 2960839

Gene Structure 6059 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1040600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3276 bp

ATGTCGCCGCCGATGAGTCTCTCTGCAACTCACCTTACCACTTGTCGCTTCCCGCCAAAAACCATAACCGACCACCCATCACTCTCTCCCTTCTTTTCCTTTCCCAGAACCCTCCCCTCCCAAAATCCTAAATCCTCTAAATTCACAATTCGGAGCTCCGCCGCCGACACCAAAACACTTGCGCCCAAGTCTGCCATTCAGAGAATCGCCGAGAAGCTCCGAAGCCTCGGGTTCACCGAGAGCACCCAAAATCCCGACCGGCAACCCGATAAGAAGTCCGCCGGCGAAATCTTTGTTCCGATTCCGGAGAAATTGCCCAAGTACCGGGTCGGGCACACGCTCGATGCAAGCTGGAGCACGCCCGAGAACCCGGTCCCAGAACCGGGCACCGGCGGAGCGATTGCGAGGTTTCATGAGTTGAGGCGGGAGGTGAAGAAGCAGAAGGAATTAGAGAGGGAGGAGAGGAAGGGGAAGGGGGAGGGGAGGGTTCCGACATTGGCGGAGCTGAGCTTGGGGAAGGGTGAGTTGAGGCGGCTGACGACGGTTGGGCTTGGGCTGAGGAAGAAGCTGAAGATTGGGAAGGCTGGGATTACTGAAGGGATTGTGAATGGGATTCACGAGAACTGGCGGCGGTCGGAGGTTGTGAAGATTGTGTGTGAGGATTTGTGTAGGTTGAACATGAAGAGGACTCATGACTTGTTGGAGAGAAAAACTGGAGGGCTGGTTGTCTGGAGGGCTGGAAGTAAGATAATTTTGTATAGGGGGGTGAACTATAAGTATCCATACTTTTTGCGTGACAATGTTCGCAAAGATTCAATGAGTGAGGCTAACTCTATTGCACTACCTGATACACACATCAATGATGGAGGAAATGAGAGTGGTACATCCAGCAAAAATGATGTAAACTCTGCTATAGTACCAAGTACAAGCTATGAACGAGCTCAACCAGCGTTAGTACAAGGTGTTGGTTTGCCAGGTAGAGTTCGGTTTCAACTTCCAGGCGAGGCACAACTTTCAGAAGAAAATGACAGCATGTTAGAGGGACTGGGGCCGCGGTTTACTGATTGGTGGGGTTGTGAACCTCTTCCCATTGATGCTGACCTTCTACCAGCCATTGTTCCTGGATACAGGAAACCTTTCCGCCTTCTTCCCTATGGTCTGAACTCTAAACTAACAGATGATGAAATGACCACAATAAGAAGACTCGGTCGACCCTTACCATGTCATTTTGCATTGGGCAGAAACAGAAATCTTCAGGGTTTGGCCGCTTCCATTGTCAAACTCTGGGAAAAATGTGAGATTGCCAAAATAGCTGTTAAAAGAGGGGTACAGAACACTAACAGCGAGATGATGGCTGAAGAGCTAAAGAGGCTGACTGGAGGAACTCTGCTTGCTCGGGACAGGGAATTTATAGTTTTGTATAGGGGAAAGGATTTTCTGCCACCTGCAGTTTCATCTGCAATAGAAGAACGGAGGAGGCATGCAATACGTGTAGAGCGTAGTACCTCAGTTCCAACTAGACAGGATCTTGAACCCAGGACTGAGCCAGAAAATAAACATGGCTGGATCGATGATCATAAAATGGGGCTTCCATCCGCAAGAAAGCTAAAGTCTACTGAGGCAGCTGTCGATAGGACCAGCATTAAATTGTCCATGGCTTTAGAAAAGAGAGTAAAGGCAGAAAAACTTCTAGCAGATCTGGAGAACGCAGCGACCCCCCAACAACCAGAAATAGATAAAGAAGGTATTACTAAAGAAGAAAGATATATGCTAAGGAAGGTTGGCTTGAGAATGAAGCCTTTCCTACTGATGGGTAGGCGAGGAGTATTTGATGGAACAATTGAAAACATGCATCTTCATTGGAAGTATAGGGAACTCGTTAAGATAATATCTAATGAGAAAAGTATCGAAACTGTTCACCAAGTAGCACAAACCTTAGAGGCAGAAAGTGGTGGGATACTAGTGGCAGTGGAGAGAGTGAGTAAGGGTTATGCAATTATTGTGTATCGCGGAAAAAATTATAGCAGGCCTGCTTCTTTGAGACCTCAGACACTTCTGACTAAAAGAGAAGCAATGAAACGTTCTATTGAGGCACAGCGTCGTGAGTCTTTGAAACTTCATGTTTTAAAGCTTAACGAAAATATAGCTGAATTAAAGCTTCAGTTGGATGAAGACAAGGAATCAGACAATACGCACTCGGTTAATGAATCAAGAACTCAATTGGCTAGAGACAAACAAGAAGCTCATGTGATTCCCATAAGTTTGAATGATGGAGTGGCACCTGTTGTGAACGGTCAATTAGCCACGCAACAAGACAATATGACGAGTTTCTCTCCGACCTGTGACGAGGATGAATCTGGGAGAGTTGAGCCAGGATCTTCAAATGAATCAGTTACAAATGAAGAGGATGAAACTAGTACAGTTGAGACAGGATCTTCAAATGAACCAGTTACATATGAAAGTCGTGCAAGTTCGCTCAAGGATATAAATGGAGAAGTTGAAGGCTTCCCATCTACGTGTTGTCCTGAAGATGTTCTGGCTAGAAACAAACAAGAAGGTCATGCTACTCCCATGAAATTGGAAGATAGAACGGGCACTGTAGTAAATGGTCAAGTAGCCACACAGCAGGATAAAGTCCTTACTTTCTCTTCGATCTGGGGTGAGGATGAAAATGGTAAAGCTGAGCCACCCGTCTCTAAATCTTTCTCGACAAACCGTTTGAGCAGTTCAGCGACTGTGGTCAAACATCGTCGTGATAATGAGGCCAGGGACTCATCACGCAGACCTGGCAATAGTGAATCAGAACCATCAGTCCCAGTACTGGTAAGAAAGAATTTTAATGAGAGGCCCTCAAAGGCGGTTCATCTTTCCAACAATGAGAGGCTTCTTCTGCGAAAACAAGCCCTCAAGATGAAAAACCGCCCTGTGATAGCAGTTGGAAGGAGCAACATTGTAACTGGTGTTGCTAAAACAATCAAGGCACACTTTGAGAAGCACCCTCTTGCCATAGTGAACGTCAAAGGCAGAGCAAAAGGAACTTCGGTCAGGGAAGTTGTTTTCAAACTGGAGCAAGCAACGGGTGCGGTTTTAGTCTCCCAGGAGCCTAGCAAAGTTATACTTTACCGGGGCTGGGGCGCAGGAGACAATCCTCGCCGTAATGGAAGTAACACAGGGAAAATTGCTTCTTCTCAGGGTGCCGTGTCTCCCGAACTCCTGGCGGCAATAAGACTCGAGTGCGGTTTTAAACCTCGCGAGGGCGAAGATGCAGCTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000372 GO:0000373 GO:0000375 GO:0000376 GO:0000377 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009507 GO:0009532 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CRS1_YhbY

Protein Analysis

1092

Amino Acids

121.12

Weight (kDa)

9.43

Isoelectric Point (pI)

45.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CRS1_YhbY	PF01985	174 - 254	7.1e-21	CRS1 / YhbY (CRM) domain
CRS1_YhbY	PF01985	391 - 475	7.2e-16	CRS1 / YhbY (CRM) domain
CRS1_YhbY	PF01985	583 - 670	1.4e-18	CRS1 / YhbY (CRM) domain
CRS1_YhbY	PF01985	956 - 1042	7.4e-15	CRS1 / YhbY (CRM) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007157)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01370
fragaria_vesca	FvH4_6g50050 FvH4_6g50050 FvH4_6g50050 FvH4_6g50050 FvH4_6g50050 FvH4_6g50050 FvH4_6g50050 FvH4_6g50050 FvH4_6g50050 FvH4_6g50050
malus_domestica	MD17G1040600.v1.1
prunus_persica	Prupe.3G278400_v2.0.a1
pyrus_communis	pycom17g03640
rosa_chinensis	RchiOBHm_Chr2g0170301
rosa_laevigata	RLG00000021945
rosa_multiflora	Rmu_co8444867.1_g000001 Rmu_sc0012445.1_g000003
rosa_roxburghii	Rroxscaffold_2G00081450
rosa_rugosa	Rorug02G0548100
rosa_samantha	Rh2AG619400 Rh2BG630300 Rh2CG600400 Rh2DG643200
rosa_wichuraiana	Rw2G051310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1453
Acc36I	ACCTGC	1 cut(s)	1453
AccB1I	GGYRCC	3 cut(s)	386, 2278, 3194
AccI	GTMKAC	2 cut(s)	1210, 1613
AccII	CGCG	3 cut(s)	1054, 2010, 3252
AccIII	TCCGGA	1 cut(s)	304
AclI	AACGTT	1 cut(s)	2080
AclWI	GGATC	4 cut(s)	1533, 1574, 2383, 2440
AcoI	YGGCCR	1 cut(s)	1263
AcsI	RAATTY	3 cut(s)	140, 1406, 2833
AcuI	CTGAAG	6 cut(s)	590, 615, 1238, 1377, 2144, 2547
AfaI	GTAC	9 cut(s)	323, 883, 918, 925, 957, 1329, 1504, 2419, 2820
AfiI	CCNNNNNNNGG	8 cut(s)	324, 382, 383, 389, 1810, 2821, 3130, 3254
AflIII	ACRYGT	2 cut(s)	1489, 2516
AhlI	ACTAGT	2 cut(s)	1960, 2414
AjnI	CCWGG	9 cut(s)	973, 997, 1120, 1535, 2371, 2765, 2785, 3096, 3213
AloI	GAACNNNNNNTCC	2 cut(s)	2796, 2828
Alw21I	GWGCWC	4 cut(s)	158, 245, 359, 943
Alw26I	GTCTC	8 cut(s)	23, 2035, 2223, 2420, 2698, 3097, 3135, 3207
AlwI	GGATC	4 cut(s)	1533, 1574, 2383, 2440
AlwNI	CAGNNNCTG	1 cut(s)	2735
Ama87I	CYCGRG	4 cut(s)	227, 362, 1395, 3230
Aor13HI	TCCGGA	1 cut(s)	304
AoxI	GGCC	5 cut(s)	1049, 1263, 2027, 2763, 2846
ApeKI	GCWGC	4 cut(s)	1622, 1709, 3266, 3269
ApoI	RAATTY	3 cut(s)	140, 1406, 2833
ArsI	GACNNNNNNTTYG	2 cut(s)	2701, 2733
Asp700I	GAANNNNTTC	6 cut(s)	1083, 1136, 1686, 1799, 1914, 2532
AspLEI	GCGC	2 cut(s)	184, 3137
AspS9I	GGNCC	4 cut(s)	373, 1049, 1635, 2847
AsuC2I	CCSGG	4 cut(s)	326, 371, 384, 3125
AsuHPI	GGTGA	7 cut(s)	23, 226, 442, 533, 778, 1910, 2672
AvaI	CYCGRG	4 cut(s)	227, 362, 1395, 3230
AvaII	GGWCC	2 cut(s)	373, 1635
BaeGI	GKGCMC	3 cut(s)	336, 389, 2597
BanI	GGYRCC	3 cut(s)	386, 2278, 3194
BanII	GRGCYC	2 cut(s)	158, 943
BauI	CACGAG	1 cut(s)	617
BbsI	GAAGAC	2 cut(s)	1207, 2178
Bbv12I	GWGCWC	4 cut(s)	158, 245, 359, 943
BbvCI	CCTCAGC	1 cut(s)	557
BbvI	GCAGC	3 cut(s)	1634, 1721, 3256
BccI	CCATC	9 cut(s)	85, 857, 1345, 1600, 1804, 1826, 2264, 2518, 2815
BceAI	ACGGC	2 cut(s)	3138, 3182
BciT130I	CCWGG	9 cut(s)	975, 999, 1122, 1537, 2373, 2767, 2787, 3098, 3215
BciVI	GTATCC	3 cut(s)	789, 1118, 1950
BclI	TGATCA	1 cut(s)	1573
BcnI	CCSGG	4 cut(s)	326, 371, 384, 3125
BcoDI	GTCTC	8 cut(s)	23, 2035, 2223, 2420, 2698, 3097, 3135, 3207
BcuI	ACTAGT	2 cut(s)	1960, 2414
BfaI	CTAG	7 cut(s)	1518, 1691, 1961, 2226, 2415, 2541, 3105
BfmI	CTRYAG	3 cut(s)	912, 1446, 2598
BfuAI	ACCTGC	1 cut(s)	1453
BfuI	GTATCC	3 cut(s)	789, 1118, 1950
BglII	AGATCT	1 cut(s)	1696
BlpI	GCTNAGC	2 cut(s)	506, 2682
BmcAI	AGTACT	1 cut(s)	2820
Bme18I	GGWCC	2 cut(s)	373, 1635
BmeT110I	CYCGRG	4 cut(s)	227, 362, 1395, 3230
BmgT120I	GGNCC	4 cut(s)	373, 1049, 1635, 2847
BmiI	GGNNCC	7 cut(s)	375, 388, 489, 1050, 2280, 3102, 3196
BmrI	ACTGGG	2 cut(s)	1055, 2810
BmsI	GCATC	4 cut(s)	334, 1084, 1861, 3253
BmuI	ACTGGG	2 cut(s)	1055, 2810
BoxI	GACNNNNGTC	1 cut(s)	2738
BpiI	GAAGAC	2 cut(s)	1207, 2178
BplI	GAGNNNNNCTC	6 cut(s)	815, 847, 2317, 2349, 2834, 2866
BpmI	CTGGAG	6 cut(s)	373, 735, 751, 1398, 1721, 3086
Bpu10I	CCTNAGC	2 cut(s)	557, 1772
Bpu1102I	GCTNAGC	2 cut(s)	506, 2682
BpuEI	CTTGAG	3 cut(s)	1807, 2462, 2891
BpuMI	CCSGG	4 cut(s)	326, 371, 384, 3125
Bsa29I	ATCGAT	1 cut(s)	1569
BsaAI	YACGTR	2 cut(s)	1490, 2517
BsaI	GGTCTC	1 cut(s)	2035
BsaJI	CCNNGG	7 cut(s)	226, 1052, 1535, 1653, 2766, 3096, 3124
BsaWI	WCCGGW	1 cut(s)	304
BsaXI	ACNNNNNCTCC	2 cut(s)	708, 738
Bsc4I	CCNNNNNNNGG	8 cut(s)	324, 382, 383, 389, 1810, 2821, 3130, 3254
Bse118I	RCCGGY	3 cut(s)	258, 281, 389
Bse1I	ACTGG	9 cut(s)	629, 718, 1050, 1381, 2447, 2816, 2826, 2962, 3069
Bse3DI	GCAATG	1 cut(s)	2079
BseAI	TCCGGA	1 cut(s)	304
BseBI	CCWGG	9 cut(s)	975, 999, 1122, 1537, 2373, 2767, 2787, 3098, 3215
BseCI	ATCGAT	1 cut(s)	1569
BseDI	CCNNGG	7 cut(s)	226, 1052, 1535, 1653, 2766, 3096, 3124
BseGI	GGATG	6 cut(s)	884, 1592, 2173, 2356, 2413, 2671
BseLI	CCNNNNNNNGG	8 cut(s)	324, 382, 383, 389, 1810, 2821, 3130, 3254
BseMI	GCAATG	1 cut(s)	2079
BseMII	CTCAG	8 cut(s)	497, 548, 1521, 1533, 1608, 2060, 2673, 3203
BseNI	ACTGG	9 cut(s)	629, 718, 1050, 1381, 2447, 2816, 2826, 2962, 3069
BseRI	GAGGAG	3 cut(s)	473, 1489, 1836
BseSI	GKGCMC	3 cut(s)	336, 389, 2597
BseXI	GCAGC	3 cut(s)	1634, 1721, 3256
BseYI	CCCAGC	2 cut(s)	584, 3129
Bsh1236I	CGCG	3 cut(s)	1054, 2010, 3252
Bsh1285I	CGRYCG	3 cut(s)	259, 635, 1210
BshFI	GGCC	5 cut(s)	1051, 1265, 2029, 2765, 2848
BshNI	GGYRCC	3 cut(s)	386, 2278, 3194
BshVI	ATCGAT	1 cut(s)	1569
BsiEI	CGRYCG	3 cut(s)	259, 635, 1210
BsiHKAI	GWGCWC	4 cut(s)	158, 245, 359, 943
BsiHKCI	CYCGRG	4 cut(s)	227, 362, 1395, 3230
BsiSI	CCGG	8 cut(s)	259, 282, 305, 325, 371, 383, 390, 3124
BslFI	GGGAC	5 cut(s)	359, 1056, 1412, 2783, 2798
BslI	CCNNNNNNNGG	8 cut(s)	324, 382, 383, 389, 1810, 2821, 3130, 3254
BsmAI	GTCTC	8 cut(s)	23, 2035, 2223, 2420, 2698, 3097, 3135, 3207
BsmBI	CGTCTC	1 cut(s)	2698
BsmFI	GGGAC	5 cut(s)	359, 1056, 1412, 2783, 2798
BsnI	GGCC	5 cut(s)	1051, 1265, 2029, 2765, 2848
Bso31I	GGTCTC	1 cut(s)	2035
BsoBI	CYCGRG	4 cut(s)	227, 362, 1395, 3230
Bsp1286I	GDGCHC	7 cut(s)	158, 245, 336, 359, 389, 943, 2597
Bsp13I	TCCGGA	1 cut(s)	304
Bsp143I	GATC	7 cut(s)	1525, 1566, 1573, 1696, 2375, 2432, 2652
Bsp1720I	GCTNAGC	2 cut(s)	506, 2682
Bsp19I	CCATGG	1 cut(s)	1653
Bsp68I	TCGCGA	1 cut(s)	3252
BspANI	GGCC	5 cut(s)	1051, 1265, 2029, 2765, 2848
BspCNI	CTCAG	8 cut(s)	498, 549, 1520, 1534, 1609, 2059, 2674, 3202
BspDI	ATCGAT	1 cut(s)	1569
BspEI	TCCGGA	1 cut(s)	304
BspFNI	CGCG	3 cut(s)	1054, 2010, 3252
BspHI	TCATGA	2 cut(s)	412, 691
BspLI	GGNNCC	7 cut(s)	375, 388, 489, 1050, 2280, 3102, 3196
BspMAI	CTGCAG	1 cut(s)	1450
BspMI	ACCTGC	1 cut(s)	1453
BspPI	GGATC	4 cut(s)	1533, 1574, 2383, 2440
BspQI	GCTCTTC	1 cut(s)	1353
BspT107I	GGYRCC	3 cut(s)	386, 2278, 3194
BspTNI	GGTCTC	1 cut(s)	2035
BsrDI	GCAATG	1 cut(s)	2079
BsrFI	RCCGGY	3 cut(s)	258, 281, 389
BsrI	ACTGG	9 cut(s)	629, 718, 1050, 1381, 2447, 2816, 2826, 2962, 3069
BssAI	RCCGGY	3 cut(s)	258, 281, 389
BssECI	CCNNGG	7 cut(s)	226, 1052, 1535, 1653, 2766, 3096, 3124
BssMI	GATC	7 cut(s)	1525, 1566, 1573, 1696, 2375, 2432, 2652
BssSI	CACGAG	1 cut(s)	617
BssT1I	CCWWGG	1 cut(s)	1653
Bst2BI	CACGAG	1 cut(s)	617
Bst2UI	CCWGG	9 cut(s)	975, 999, 1122, 1537, 2373, 2767, 2787, 3098, 3215
Bst4CI	ACNGT	7 cut(s)	544, 1915, 2294, 2422, 2599, 2717, 2737
Bst6I	CTCTTC	5 cut(s)	677, 1089, 1353, 2397, 2653
BstAPI	GCANNNNNTGC	1 cut(s)	3080
BstBAI	YACGTR	2 cut(s)	1490, 2517
BstC8I	GCNNGC	8 cut(s)	283, 349, 359, 1392, 1482, 2027, 2031, 3072
BstDSI	CCRYGG	2 cut(s)	1052, 1653
BstF5I	GGATG	6 cut(s)	884, 1592, 2173, 2356, 2413, 2671
BstFNI	CGCG	3 cut(s)	1054, 2010, 3252
BstHHI	GCGC	2 cut(s)	184, 3137
BstKTI	GATC	7 cut(s)	1528, 1569, 1576, 1699, 2378, 2435, 2655
BstMAI	GTCTC	8 cut(s)	23, 2035, 2223, 2420, 2698, 3097, 3135, 3207
BstMBI	GATC	7 cut(s)	1525, 1566, 1573, 1696, 2375, 2432, 2652
BstMCI	CGRYCG	3 cut(s)	259, 635, 1210
BstMWI	GCNNNNNNNGC	6 cut(s)	947, 1310, 1369, 2787, 2890, 3080
BstNI	CCWGG	9 cut(s)	975, 999, 1122, 1537, 2373, 2767, 2787, 3098, 3215
BstNSI	RCATGY	3 cut(s)	1036, 1484, 1852
BstPAI	GACNNNNGTC	1 cut(s)	2738
BstSFI	CTRYAG	3 cut(s)	912, 1446, 2598
BstSLI	GKGCMC	3 cut(s)	336, 389, 2597
BstUI	CGCG	3 cut(s)	1054, 2010, 3252
BstV1I	GCAGC	3 cut(s)	1634, 1721, 3256
BstV2I	GAAGAC	2 cut(s)	1207, 2178
BstX2I	RGATCY	4 cut(s)	1525, 1696, 2375, 2432
BstXI	CCANNNNNNTGG	1 cut(s)	2578
BstYI	RGATCY	4 cut(s)	1525, 1696, 2375, 2432
Bsu15I	ATCGAT	1 cut(s)	1569
BsuI	GTATCC	3 cut(s)	789, 1118, 1950
BsuRI	GGCC	5 cut(s)	1051, 1265, 2029, 2765, 2848
BsuTUI	ATCGAT	1 cut(s)	1569
BtgI	CCRYGG	2 cut(s)	1052, 1653
BtsCI	GGATG	6 cut(s)	884, 1592, 2173, 2356, 2413, 2671
BtsI	GCAGTG	1 cut(s)	1974
BtsIMutI	CAGTG	2 cut(s)	1974, 2595
BtuMI	TCGCGA	1 cut(s)	3252
BveI	ACCTGC	1 cut(s)	1453
Cac8I	GCNNGC	8 cut(s)	283, 349, 359, 1392, 1482, 2027, 2031, 3072
CaiI	CAGNNNCTG	1 cut(s)	2735
CciI	TCATGA	2 cut(s)	412, 691
CfoI	GCGC	2 cut(s)	184, 3137
Cfr10I	RCCGGY	3 cut(s)	258, 281, 389
Cfr13I	GGNCC	4 cut(s)	373, 1049, 1635, 2847
Cfr42I	CCGCGG	1 cut(s)	1055
ClaI	ATCGAT	1 cut(s)	1569
CseI	GACGC	1 cut(s)	2087
Csp6I	GTAC	9 cut(s)	322, 882, 917, 924, 956, 1328, 1503, 2418, 2819
CviQI	GTAC	9 cut(s)	322, 882, 917, 924, 956, 1328, 1503, 2418, 2819
DpnI	GATC	7 cut(s)	1527, 1568, 1575, 1698, 2377, 2434, 2654
DpnII	GATC	7 cut(s)	1525, 1566, 1573, 1696, 2375, 2432, 2652
DraI	TTTAAA	2 cut(s)	2127, 3244
EaeI	YGGCCR	1 cut(s)	1263
Eam1104I	CTCTTC	5 cut(s)	677, 1089, 1353, 2397, 2653
EarI	CTCTTC	5 cut(s)	677, 1089, 1353, 2397, 2653
EciI	GGCGGA	5 cut(s)	148, 268, 408, 515, 1130
Ecl136II	GAGCTC	2 cut(s)	156, 941
Eco130I	CCWWGG	1 cut(s)	1653
Eco147I	AGGCCT	1 cut(s)	2029
Eco24I	GRGCYC	2 cut(s)	158, 943
Eco31I	GGTCTC	1 cut(s)	2035
Eco47I	GGWCC	2 cut(s)	373, 1635
Eco53kI	GAGCTC	2 cut(s)	156, 941
Eco57I	CTGAAG	6 cut(s)	590, 615, 1238, 1377, 2144, 2547
Eco88I	CYCGRG	4 cut(s)	227, 362, 1395, 3230
EcoICRI	GAGCTC	2 cut(s)	156, 941
EcoO109I	RGGNCCY	1 cut(s)	2847
EcoRII	CCWGG	9 cut(s)	973, 997, 1120, 1535, 2371, 2765, 2785, 3096, 3213
EcoT14I	CCWWGG	1 cut(s)	1653
EcoT22I	ATGCAT	1 cut(s)	1854
EcoT38I	GRGCYC	2 cut(s)	158, 943
ErhI	CCWWGG	1 cut(s)	1653
Esp3I	CGTCTC	1 cut(s)	2698
FalI	AAGNNNNNCTT	6 cut(s)	1769, 1801, 2969, 3001, 3102, 3134
FaqI	GGGAC	5 cut(s)	359, 1056, 1412, 2783, 2798
FauI	CCCGC	2 cut(s)	60, 417
FauNDI	CATATG	1 cut(s)	2455
FbaI	TGATCA	1 cut(s)	1573
FblI	GTMKAC	2 cut(s)	1210, 1613
FokI	GGATG	6 cut(s)	871, 1579, 2180, 2363, 2420, 2678
FriOI	GRGCYC	2 cut(s)	158, 943
FspBI	CTAG	7 cut(s)	1518, 1691, 1961, 2226, 2415, 2541, 3105
GlaI	GCGC	2 cut(s)	183, 3136
GsaI	CCCAGC	2 cut(s)	588, 3133
GsuI	CTGGAG	6 cut(s)	373, 735, 751, 1398, 1721, 3086
HaeIII	GGCC	5 cut(s)	1051, 1265, 2029, 2765, 2848
HapII	CCGG	8 cut(s)	259, 282, 305, 325, 371, 383, 390, 3124
HgaI	GACGC	1 cut(s)	2087
HhaI	GCGC	2 cut(s)	184, 3137
Hin6I	GCGC	2 cut(s)	182, 3135
HinP1I	GCGC	2 cut(s)	182, 3135
HincII	GTYRAC	1 cut(s)	1211
HindII	GTYRAC	1 cut(s)	1211
HindIII	AAGCTT	2 cut(s)	2129, 2156
HpaII	CCGG	8 cut(s)	259, 282, 305, 325, 371, 383, 390, 3124
HphI	GGTGA	7 cut(s)	23, 226, 442, 533, 778, 1910, 2672
Hpy99I	CGWCG	3 cut(s)	544, 2103, 2754
HpyCH4III	ACNGT	7 cut(s)	544, 1915, 2294, 2422, 2599, 2717, 2737
HpyCH4IV	ACGT	4 cut(s)	1489, 2080, 2516, 3015
HpyF10VI	GCNNNNNNNGC	6 cut(s)	947, 1310, 1369, 2787, 2890, 3080
HpySE526I	ACGT	4 cut(s)	1489, 2080, 2516, 3015
HspAI	GCGC	2 cut(s)	182, 3135
Kpn2I	TCCGGA	1 cut(s)	304
KroI	GCCGGC	1 cut(s)	281
KroNI	GCCGGC	1 cut(s)	283
Ksp22I	TGATCA	1 cut(s)	1573
KspI	CCGCGG	1 cut(s)	1055
Kzo9I	GATC	7 cut(s)	1525, 1566, 1573, 1696, 2375, 2432, 2652
LguI	GCTCTTC	1 cut(s)	1353
LmnI	GCTCC	9 cut(s)	153, 161, 222, 354, 395, 502, 2943, 3067, 3100
Lsp1109I	GCAGC	3 cut(s)	1634, 1721, 3256
LweI	GCATC	4 cut(s)	334, 1084, 1861, 3253
MaeI	CTAG	7 cut(s)	1518, 1691, 1961, 2226, 2415, 2541, 3105
MaeII	ACGT	4 cut(s)	1489, 2080, 2516, 3015
MaeIII	GTNAC	6 cut(s)	794, 2342, 2392, 2449, 2953, 3164
MalI	GATC	7 cut(s)	1527, 1568, 1575, 1698, 2377, 2434, 2654
MboI	GATC	7 cut(s)	1525, 1566, 1573, 1696, 2375, 2432, 2652
MfeI	CAATTG	2 cut(s)	1839, 2219
MflI	RGATCY	4 cut(s)	1525, 1696, 2375, 2432
MhlI	GDGCHC	7 cut(s)	158, 245, 336, 359, 389, 943, 2597
MlyI	GAGTC	6 cut(s)	25, 682, 1197, 2114, 2765, 3222
MmeI	TCCRAC	8 cut(s)	515, 615, 681, 1538, 2145, 2360, 2895, 2917
Mph1103I	ATGCAT	1 cut(s)	1854
MreI	CGCCGGCG	1 cut(s)	281
MroI	TCCGGA	1 cut(s)	304
MroNI	GCCGGC	1 cut(s)	281
MroXI	GAANNNNTTC	6 cut(s)	1083, 1136, 1686, 1799, 1914, 2532
MseI	TTAA	9 cut(s)	1317, 1644, 1881, 2126, 2133, 2153, 2202, 2838, 3243
MslI	CAYNNNNRTG	3 cut(s)	858, 2515, 2751
MspA1I	CMGCKG	3 cut(s)	1054, 1625, 3269
MspI	CCGG	8 cut(s)	259, 282, 305, 325, 371, 383, 390, 3124
MunI	CAATTG	2 cut(s)	1839, 2219
MvaI	CCWGG	9 cut(s)	975, 999, 1122, 1537, 2373, 2767, 2787, 3098, 3215
MvnI	CGCG	3 cut(s)	1054, 2010, 3252
MwoI	GCNNNNNNNGC	6 cut(s)	947, 1310, 1369, 2787, 2890, 3080
NaeI	GCCGGC	1 cut(s)	283
NciI	CCSGG	4 cut(s)	326, 371, 384, 3125
NcoI	CCATGG	1 cut(s)	1653
NdeI	CATATG	1 cut(s)	2455
NdeII	GATC	7 cut(s)	1525, 1566, 1573, 1696, 2375, 2432, 2652
NgoMIV	GCCGGC	1 cut(s)	281
NlaIV	GGNNCC	7 cut(s)	375, 388, 489, 1050, 2280, 3102, 3196
NmeAIII	GCCGAG	1 cut(s)	235
NmuCI	GTSAC	2 cut(s)	794, 2342
NruI	TCGCGA	1 cut(s)	3252
NsiI	ATGCAT	1 cut(s)	1854
NspI	RCATGY	3 cut(s)	1036, 1484, 1852
PaeI	GCATGC	1 cut(s)	1484
PaeR7I	CTCGAG	1 cut(s)	3230
PagI	TCATGA	2 cut(s)	412, 691
PaqCI	CACCTGC	1 cut(s)	1453
PceI	AGGCCT	1 cut(s)	2029
PciSI	GCTCTTC	1 cut(s)	1353
PcsI	WCGNNNNNNNCGW	2 cut(s)	234, 1495
PdiI	GCCGGC	1 cut(s)	283
PdmI	GAANNNNTTC	6 cut(s)	1083, 1136, 1686, 1799, 1914, 2532
PflFI	GACNNNGTC	1 cut(s)	1206
PfoI	TCCNGGA	1 cut(s)	1120
PleI	GAGTC	6 cut(s)	24, 682, 1197, 2113, 2765, 3222
PpsI	GAGTC	6 cut(s)	24, 682, 1197, 2113, 2765, 3222
Ppu21I	YACGTR	2 cut(s)	1490, 2517
PshAI	GACNNNNGTC	1 cut(s)	2738
Psp124BI	GAGCTC	2 cut(s)	158, 943
Psp1406I	AACGTT	1 cut(s)	2080
Psp6I	CCWGG	9 cut(s)	973, 997, 1120, 1535, 2371, 2765, 2785, 3096, 3213
PspFI	CCCAGC	2 cut(s)	584, 3129
PspGI	CCWGG	9 cut(s)	973, 997, 1120, 1535, 2371, 2765, 2785, 3096, 3213
PspN4I	GGNNCC	7 cut(s)	375, 388, 489, 1050, 2280, 3102, 3196
PspPI	GGNCC	4 cut(s)	373, 1049, 1635, 2847
PspXI	VCTCGAGB	1 cut(s)	3230
PstI	CTGCAG	1 cut(s)	1450
PstNI	CAGNNNCTG	1 cut(s)	2735
PsuI	RGATCY	4 cut(s)	1525, 1696, 2375, 2432
PsyI	GACNNNGTC	1 cut(s)	1206
PvuII	CAGCTG	2 cut(s)	1625, 3269
RruI	TCGCGA	1 cut(s)	3252
RsaI	GTAC	9 cut(s)	323, 883, 918, 925, 957, 1329, 1504, 2419, 2820
RsaNI	GTAC	9 cut(s)	322, 882, 917, 924, 956, 1328, 1503, 2418, 2819
RseI	CAYNNNNRTG	3 cut(s)	858, 2515, 2751
SacI	GAGCTC	2 cut(s)	158, 943
SacII	CCGCGG	1 cut(s)	1055
SalI	GTCGAC	1 cut(s)	1209
SapI	GCTCTTC	1 cut(s)	1353
SaqAI	TTAA	9 cut(s)	1317, 1644, 1881, 2126, 2133, 2153, 2202, 2838, 3243
Sau3AI	GATC	7 cut(s)	1525, 1566, 1573, 1696, 2375, 2432, 2652
Sau96I	GGNCC	4 cut(s)	373, 1049, 1635, 2847
ScaI	AGTACT	1 cut(s)	2820
SchI	GAGTC	6 cut(s)	25, 682, 1197, 2114, 2765, 3222
SduI	GDGCHC	7 cut(s)	158, 245, 336, 359, 389, 943, 2597
SfaNI	GCATC	4 cut(s)	334, 1084, 1861, 3253
SfcI	CTRYAG	3 cut(s)	912, 1446, 2598
Sfr274I	CTCGAG	1 cut(s)	3230
Sfr303I	CCGCGG	1 cut(s)	1055
SgrAI	CRCCGGYG	2 cut(s)	281, 389
SgrBI	CCGCGG	1 cut(s)	1055
SinI	GGWCC	2 cut(s)	373, 1635
SlaI	CTCGAG	1 cut(s)	3230
SmiMI	CAYNNNNRTG	3 cut(s)	858, 2515, 2751
SmlI	CTYRAG	4 cut(s)	1786, 2477, 2906, 3230
SmoI	CTYRAG	4 cut(s)	1786, 2477, 2906, 3230
SpeI	ACTAGT	2 cut(s)	1960, 2414
SphI	GCATGC	1 cut(s)	1484
SseBI	AGGCCT	1 cut(s)	2029
SspMI	CTAG	7 cut(s)	1518, 1691, 1961, 2226, 2415, 2541, 3105
SstI	GAGCTC	2 cut(s)	158, 943
StuI	AGGCCT	1 cut(s)	2029
StyI	CCWWGG	1 cut(s)	1653
TaaI	ACNGT	7 cut(s)	544, 1915, 2294, 2422, 2599, 2717, 2737
TaiI	ACGT	4 cut(s)	1492, 2083, 2519, 3018
TaqI	TCGA	8 cut(s)	342, 1210, 1569, 1629, 1908, 2651, 2708, 3231
TaqII	GACCGA	2 cut(s)	1196, 3031
TatI	WGTACW	4 cut(s)	923, 955, 2417, 2818
TauI	GCSGC	7 cut(s)	10, 164, 535, 631, 1054, 1268, 3221
Tru1I	TTAA	9 cut(s)	1317, 1644, 1881, 2126, 2133, 2153, 2202, 2838, 3243
Tru9I	TTAA	9 cut(s)	1317, 1644, 1881, 2126, 2133, 2153, 2202, 2838, 3243
TscAI	CASTG	2 cut(s)	1974, 2602
TseFI	GTSAC	2 cut(s)	794, 2342
TseI	GCWGC	4 cut(s)	1622, 1709, 3266, 3269
Tsp45I	GTSAC	2 cut(s)	794, 2342
TspGWI	ACGGA	1 cut(s)	1486
TspRI	CASTG	2 cut(s)	1974, 2602
Tth111I	GACNNNGTC	1 cut(s)	1206
VpaK11BI	GGWCC	2 cut(s)	373, 1635
XapI	RAATTY	3 cut(s)	140, 1406, 2833
XceI	RCATGY	3 cut(s)	1036, 1484, 1852
XhoI	CTCGAG	1 cut(s)	3230
XmiI	GTMKAC	2 cut(s)	1210, 1613
XmnI	GAANNNNTTC	6 cut(s)	1083, 1136, 1686, 1799, 1914, 2532
XspI	CTAG	7 cut(s)	1518, 1691, 1961, 2226, 2415, 2541, 3105
ZrmI	AGTACT	1 cut(s)	2820
Zsp2I	ATGCAT	1 cut(s)	1854

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.