MD17G1064400.v1.1

NADH-ubiquinone reductase complex 1 MLRQ subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015346
Physical Location & Seq
Forward (+)
5220009 .. 5220953
945 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1064400.v1.1.491

Sequence Viewer

Length: 261 bp
ATGGGGCGTTGGGTTAGGCCTGAGGTGTACCCGTTAATGGCTGCAATGACATTTGTGACAAGCTTGTGCGCATTTCAGCTTACAAGGAACATGTTCCTCAACCCTGAAGTCAGAATCAACAAAGCTCACCGTCGAATGGCGGTGCTTGAAAACGAAGAAGAGGGAGAGAAGTACGCCGAGCACGGCCTCCGGAAGTTCCTCCGCACACGGCCACCGGAGATCATGCCTACCATCAACCACTTCTTCTCTCAAGAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B12D

Protein Analysis

87

Amino Acids

10.24

Weight (kDa)

9.42

Isoelectric Point (pI)

63.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B12D PF06522 4 - 58 7.4e-21 NADH-ubiquinone reductase complex 1 MLRQ subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015346)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29970 AT3G29970 AT3G29970
fragaria_vesca FvH4_6g46480
malus_domestica MD09G1073000.v1.1 MD17G1064400.v1.1
prunus_persica Prupe.3G250200_v2.0.a1
pyrus_communis pycom17g06450
rosa_chinensis RchiOBHm_Chr2g0165051
rosa_multiflora Rmu_sc0001097.1_g000024
rosa_roxburghii Rroxscaffold_2G00086090
rosa_rugosa Rorug02G0512400
rosa_samantha Rh2AG580600 Rh2CG562500 Rh2DG602000
rosa_wichuraiana Rw2G048200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 70
AccIII TCCGGA 1 cut(s) 189
AciI CCGC 2 cut(s) 140, 202
AcoI YGGCCR 1 cut(s) 209
AcuI CTGAAG 1 cut(s) 126
AfaI GTAC 2 cut(s) 29, 173
AfiI CCNNNNNNNGG 2 cut(s) 37, 136
AflIII ACRYGT 1 cut(s) 90
AgsI TTSAA 1 cut(s) 149
AluBI AGCT 3 cut(s) 63, 79, 125
AluI AGCT 3 cut(s) 63, 79, 125
Alw21I GWGCWC 1 cut(s) 183
Aor13HI TCCGGA 1 cut(s) 189
AoxI GGCC 3 cut(s) 17, 184, 209
ApeKI GCWGC 1 cut(s) 41
Asp700I GAANNNNTTC 1 cut(s) 92
AspLEI GCGC 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 119
AxyI CCTNAGG 1 cut(s) 21
Bbv12I GWGCWC 1 cut(s) 183
BbvI GCAGC 1 cut(s) 28
BccI CCATC 1 cut(s) 239
BceAI ACGGC 2 cut(s) 199, 224
BisI GCNGC 1 cut(s) 42
BlsI GCNGC 1 cut(s) 43
BpuEI CTTGAG 1 cut(s) 234
BsaWI WCCGGW 2 cut(s) 189, 214
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 136
Bse21I CCTNAGG 1 cut(s) 21
Bse3DI GCAATG 1 cut(s) 51
BseAI TCCGGA 1 cut(s) 189
BseLI CCNNNNNNNGG 2 cut(s) 37, 136
BseMI GCAATG 1 cut(s) 51
BseMII CTCAG 1 cut(s) 12
BseXI GCAGC 1 cut(s) 28
BshFI GGCC 3 cut(s) 19, 186, 211
BsiHKAI GWGCWC 1 cut(s) 183
BsiSI CCGG 2 cut(s) 190, 215
BslI CCNNNNNNNGG 2 cut(s) 37, 136
BsnI GGCC 3 cut(s) 19, 186, 211
Bsp1286I GDGCHC 1 cut(s) 183
Bsp13I TCCGGA 1 cut(s) 189
Bsp143I GATC 1 cut(s) 219
BspACI CCGC 2 cut(s) 140, 202
BspANI GGCC 3 cut(s) 19, 186, 211
BspCNI CTCAG 1 cut(s) 13
BspEI TCCGGA 1 cut(s) 189
BsrDI GCAATG 1 cut(s) 51
BssMI GATC 1 cut(s) 219
Bst4CI ACNGT 1 cut(s) 131
Bst6I CTCTTC 1 cut(s) 153
BstDEI CTNAG 1 cut(s) 21
BstHHI GCGC 1 cut(s) 71
BstKTI GATC 1 cut(s) 222
BstMBI GATC 1 cut(s) 219
BstNSI RCATGY 1 cut(s) 94
BstV1I GCAGC 1 cut(s) 28
Bsu36I CCTNAGG 1 cut(s) 21
BsuRI GGCC 3 cut(s) 19, 186, 211
CfoI GCGC 1 cut(s) 71
Csp6I GTAC 2 cut(s) 28, 172
CviAII CATG 2 cut(s) 91, 223
CviJI RGCY 7 cut(s) 19, 41, 63, 79, 125, 186, 211
CviKI_1 RGCY 7 cut(s) 19, 41, 63, 79, 125, 186, 211
CviQI GTAC 2 cut(s) 28, 172
DdeI CTNAG 1 cut(s) 21
DpnI GATC 1 cut(s) 221
DpnII GATC 1 cut(s) 219
EaeI YGGCCR 1 cut(s) 209
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
Eco147I AGGCCT 1 cut(s) 19
Eco57I CTGAAG 1 cut(s) 126
Eco81I CCTNAGG 1 cut(s) 21
FaeI CATG 2 cut(s) 94, 226
FaiI YATR 2 cut(s) 92, 224
FatI CATG 2 cut(s) 90, 222
Fnu4HI GCNGC 1 cut(s) 42
Fsp4HI GCNGC 1 cut(s) 42
FspAI RTGCGCAY 1 cut(s) 70
FspI TGCGCA 1 cut(s) 70
GlaI GCGC 1 cut(s) 70
GluI GCNGC 1 cut(s) 42
HaeIII GGCC 3 cut(s) 19, 186, 211
HapII CCGG 2 cut(s) 190, 215
HhaI GCGC 1 cut(s) 71
Hin1II CATG 2 cut(s) 94, 226
Hin6I GCGC 1 cut(s) 69
HinP1I GCGC 1 cut(s) 69
HindIII AAGCTT 1 cut(s) 61
HinfI GANTC 1 cut(s) 114
HpaII CCGG 2 cut(s) 190, 215
HphI GGTGA 1 cut(s) 119
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 1 cut(s) 113
Hpy188III TCNNGA 2 cut(s) 190, 251
Hpy8I GTNNAC 1 cut(s) 28
Hpy99I CGWCG 1 cut(s) 135
HpyCH4III ACNGT 1 cut(s) 131
HpyCH4V TGCA 1 cut(s) 44
HpyF3I CTNAG 1 cut(s) 21
Hsp92II CATG 2 cut(s) 94, 226
HspAI GCGC 1 cut(s) 69
Kpn2I TCCGGA 1 cut(s) 189
Kzo9I GATC 1 cut(s) 219
LpnPI CCDG 4 cut(s) 33, 117, 203, 228
Lsp1109I GCAGC 1 cut(s) 28
MaeIII GTNAC 1 cut(s) 55
MalI GATC 1 cut(s) 221
MboI GATC 1 cut(s) 219
MboII GAAGA 3 cut(s) 167, 170, 235
MhlI GDGCHC 1 cut(s) 183
MnlI CCTC 5 cut(s) 16, 107, 154, 197, 209
MroI TCCGGA 1 cut(s) 189
MroXI GAANNNNTTC 1 cut(s) 92
MseI TTAA 1 cut(s) 35
MspI CCGG 2 cut(s) 190, 215
NdeII GATC 1 cut(s) 219
NlaIII CATG 2 cut(s) 94, 226
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 1 cut(s) 55
NsbI TGCGCA 1 cut(s) 70
NspI RCATGY 1 cut(s) 94
PceI AGGCCT 1 cut(s) 19
PciI ACATGT 1 cut(s) 90
PdmI GAANNNNTTC 1 cut(s) 92
PfeI GAWTC 1 cut(s) 114
PkrI GCNGC 1 cut(s) 43
PscI ACATGT 1 cut(s) 90
RsaI GTAC 2 cut(s) 29, 173
RsaNI GTAC 2 cut(s) 28, 172
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 1 cut(s) 42
Sau3AI GATC 1 cut(s) 219
SduI GDGCHC 1 cut(s) 183
SetI ASST 4 cut(s) 27, 65, 81, 127
SmlI CTYRAG 1 cut(s) 249
SmoI CTYRAG 1 cut(s) 249
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 2 cut(s) 140, 202
StuI AGGCCT 1 cut(s) 19
TaaI ACNGT 1 cut(s) 131
TaqI TCGA 1 cut(s) 133
TfiI GAWTC 1 cut(s) 114
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 1 cut(s) 41
Tsp45I GTSAC 1 cut(s) 55
XceI RCATGY 1 cut(s) 94
XmnI GAANNNNTTC 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.