MD17G1110900.v1.1
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015088
Physical Location & Seq
Reverse (-)
9535780 .. 9537280
1501 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1110900.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGGGTTCGAAGTTTGTTCTTAGAGCTACATTCTTTTTGTTCTTGGTAGCACTCTTCATAAAAGCCAAGGCTGCAGATATTGATATCAAGAAGTTTGGAGCCAAGGCTGATGGAAAGACAGATGACAGCCAGGCTATTAATAGTGCTTGGAAAGAAGCCTGTACATCTGCTACGCCAAGCACGGTTGTGATAGCAAAGGGAAACTACATGGTTGGTCCAGTGAAGTTTCAAGGACCCTGCAAAGCACCGGTTAGCCTTCGGGTTGAAGGAACCCTACAGGCTCCGGCAGAACCCGAAAAGCTCAAGTCACAAGATGGGTGGGTTATTTTCCAAAACATTGATGCGTTATCAGTCTCAGGAGGTGGCACTTTTGATGGCCAAGGTTCAATTGCTTGGTCCAAAAATGATTGTGCCAAAACTGGCAAATGCAACTCGCTTCCTATTAGTTTAAGATTAACTGGGCTGACCAATGCACACATTCAAGACATAACATCTACGAACAGCAAGTTGTTCCACATGAATATCTTGAACTGCAAAAACGTGACTCTTCAACATGTGACCATCAACGCCCCTGGCGAAAGCCTAAATACCGACGGAATCCACATTGGTAGGTCAACGAACATCAACATCACCGGCGCAGAAATTCAAACCGGTGATGATTGTGTCTCCATTGGTGATGGAAGCCAGCAAATTAACATCGAGAAGGTGACGTGCGGGCCAGGCCACGGCATTAGCATTGGTAGCCTAGGCAGGTACCACGATGAGCAACCTGTAAGAGGGGTCACAGTGAGAAATTGCACCATAAAAAACACTTCCAATGGTGTTAGGGTTAAGACATGGCCTGCTTCACCTAATGGTGTGGCCTCGGATTTGCATTTCGAGGACATAATTATGGAAAACGTGACTACTTCCCCGGTCCTTATAGACCAAGAATACTGCCCGAATGGCCAATGCCAAGCGAAAATGCCGTCGCGGGTTAAAATCAGCAATGTGAGCTTCAAGAACATTAGGGGCACCTCTGCTGATCCTGTTGCTGTCAAGCTTGCTTGCAGCAAAGGCATTCCATGTCAGAATGTGGAAATTAGTGACATTAATTTGACATGCAATGGGAAAAATGGCACCTGTACATCGGTATGTTCTAATGTGAACCCTACTGTGACTGGCAAAGTTATTCCACCTGCTTGTTCTGCAAAGACTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

401

Amino Acids

42.3

Weight (kDa)

8.62

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 28 - 71 3.8e-09 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 54 - 383 1.8e-104 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07850 AT3G14040
fragaria_vesca FvH4_6g41360
malus_domestica MD17G1110900.v1.1
prunus_persica Prupe.3G203200_v2.0.a1
pyrus_communis pycom17g10330
rosa_chinensis RchiOBHm_Chr2g0156901
rosa_laevigata RLG00000020951
rosa_multiflora Rmu_ssc0000408.1_g000002
rosa_roxburghii Rroxscaffold_2G00092600
rosa_rugosa Rorug02G0456900 Rorug02G0457000
rosa_samantha Rh2AG522700 Rh2BG536000 Rh2CG507600 Rh2DG545000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1186
Acc36I ACCTGC 2 cut(s) 741, 1186
Acc65I GGTACC 1 cut(s) 753
AccB1I GGYRCC 3 cut(s) 753, 1013, 1118
AccII CGCG 1 cut(s) 973
AciI CCGC 2 cut(s) 714, 973
AclWI GGATC 1 cut(s) 1019
AcoI YGGCCR 2 cut(s) 376, 946
AcsI RAATTY 1 cut(s) 642
AfaI GTAC 3 cut(s) 163, 755, 1126
AfiI CCNNNNNNNGG 2 cut(s) 725, 776
AflIII ACRYGT 1 cut(s) 553
AgeI ACCGGT 2 cut(s) 247, 650
AgsI TTSAA 8 cut(s) 230, 266, 387, 482, 529, 551, 647, 1000
AjiI CACGTC 1 cut(s) 711
AjnI CCWGG 3 cut(s) 129, 571, 718
AleI CACNNNNGTG 1 cut(s) 185
AluBI AGCT 4 cut(s) 26, 301, 996, 1042
AluI AGCT 4 cut(s) 26, 301, 996, 1042
Alw26I GTCTC 2 cut(s) 358, 670
AlwI GGATC 1 cut(s) 1019
AoxI GGCC 6 cut(s) 376, 716, 721, 839, 861, 946
ApeKI GCWGC 2 cut(s) 71, 1050
ApoI RAATTY 1 cut(s) 642
ArsI GACNNNNNNTTYG 2 cut(s) 953, 985
AseI ATTAAT 2 cut(s) 138, 1092
AsiGI ACCGGT 2 cut(s) 247, 650
Asp718I GGTACC 1 cut(s) 753
AspA2I CCTAGG 1 cut(s) 745
AspLEI GCGC 1 cut(s) 638
AspS9I GGNCC 5 cut(s) 215, 233, 396, 716, 916
AsuC2I CCSGG 1 cut(s) 914
AsuHPI GGTGA 5 cut(s) 622, 665, 686, 718, 840
AsuII TTCGAA 1 cut(s) 8
AvaII GGWCC 4 cut(s) 215, 233, 396, 916
AvrII CCTAGG 1 cut(s) 745
BaeGI GKGCMC 1 cut(s) 1016
BalI TGGCCA 2 cut(s) 378, 948
BanI GGYRCC 3 cut(s) 753, 1013, 1118
BarI GAAGNNNNNNTAC 2 cut(s) 258, 290
BbvI GCAGC 2 cut(s) 58, 1062
BccI CCATC 5 cut(s) 104, 308, 368, 569, 671
BceAI ACGGC 2 cut(s) 742, 952
BciT130I CCWGG 3 cut(s) 131, 573, 720
BcnI CCSGG 1 cut(s) 914
BcoDI GTCTC 2 cut(s) 358, 670
BfaI CTAG 1 cut(s) 746
BfmI CTRYAG 2 cut(s) 72, 275
BfuAI ACCTGC 2 cut(s) 741, 1186
BglI GCCNNNNNGGC 1 cut(s) 945
BisI GCNGC 2 cut(s) 72, 1051
BlnI CCTAGG 1 cut(s) 745
BlsI GCNGC 2 cut(s) 73, 1052
Bme1390I CCNGG 4 cut(s) 131, 573, 720, 914
Bme18I GGWCC 4 cut(s) 215, 233, 396, 916
BmgBI CACGTC 1 cut(s) 711
BmgT120I GGNCC 5 cut(s) 215, 233, 396, 716, 916
BmiI GGNNCC 7 cut(s) 100, 235, 271, 282, 755, 1015, 1120
BmrFI CCNGG 4 cut(s) 131, 573, 720, 914
BmrI ACTGGG 1 cut(s) 468
BmsI GCATC 1 cut(s) 331
BmuI ACTGGG 1 cut(s) 468
Bpu14I TTCGAA 1 cut(s) 8
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 914
BsaJI CCNNGG 8 cut(s) 66, 102, 379, 571, 724, 745, 864, 912
BsaWI WCCGGW 2 cut(s) 247, 650
Bsc4I CCNNNNNNNGG 2 cut(s) 725, 776
Bse118I RCCGGY 3 cut(s) 247, 632, 650
Bse1I ACTGG 4 cut(s) 218, 424, 463, 1165
Bse3DI GCAATG 2 cut(s) 994, 1111
BseBI CCWGG 3 cut(s) 131, 573, 720
BseDI CCNNGG 8 cut(s) 66, 102, 379, 571, 724, 745, 864, 912
BseLI CCNNNNNNNGG 2 cut(s) 725, 776
BseMI GCAATG 2 cut(s) 994, 1111
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 4 cut(s) 218, 424, 463, 1165
BseSI GKGCMC 1 cut(s) 1016
BseXI GCAGC 2 cut(s) 58, 1062
Bsh1236I CGCG 1 cut(s) 973
BshFI GGCC 6 cut(s) 378, 718, 723, 841, 863, 948
BshNI GGYRCC 3 cut(s) 753, 1013, 1118
BshTI ACCGGT 2 cut(s) 247, 650
BsiSI CCGG 5 cut(s) 248, 284, 633, 651, 914
BslI CCNNNNNNNGG 2 cut(s) 725, 776
BsmAI GTCTC 2 cut(s) 358, 670
BsmI GAATGC 1 cut(s) 1059
BsnI GGCC 6 cut(s) 378, 718, 723, 841, 863, 948
Bsp119I TTCGAA 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 1016
Bsp1407I TGTACA 2 cut(s) 161, 1124
Bsp143I GATC 1 cut(s) 1024
BspACI CCGC 2 cut(s) 714, 973
BspANI GGCC 6 cut(s) 378, 718, 723, 841, 863, 948
BspCNI CTCAG 1 cut(s) 368
BspFNI CGCG 1 cut(s) 973
BspLI GGNNCC 7 cut(s) 100, 235, 271, 282, 755, 1015, 1120
BspMAI CTGCAG 1 cut(s) 76
BspMI ACCTGC 2 cut(s) 741, 1186
BspPI GGATC 1 cut(s) 1019
BspT104I TTCGAA 1 cut(s) 8
BspT107I GGYRCC 3 cut(s) 753, 1013, 1118
BsrDI GCAATG 2 cut(s) 994, 1111
BsrFI RCCGGY 3 cut(s) 247, 632, 650
BsrGI TGTACA 2 cut(s) 161, 1124
BsrI ACTGG 4 cut(s) 218, 424, 463, 1165
BssAI RCCGGY 3 cut(s) 247, 632, 650
BssECI CCNNGG 8 cut(s) 66, 102, 379, 571, 724, 745, 864, 912
BssMI GATC 1 cut(s) 1024
BssT1I CCWWGG 4 cut(s) 66, 102, 379, 745
Bst2UI CCWGG 3 cut(s) 131, 573, 720
Bst4CI ACNGT 3 cut(s) 184, 787, 1156
Bst6I CTCTTC 2 cut(s) 59, 552
BstAUI TGTACA 2 cut(s) 161, 1124
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 5 cut(s) 686, 716, 843, 1044, 1048
BstDEI CTNAG 2 cut(s) 20, 355
BstDSI CCRYGG 1 cut(s) 724
BstENI CCTNNNNNAGG 1 cut(s) 774
BstFNI CGCG 1 cut(s) 973
BstHHI GCGC 1 cut(s) 638
BstKTI GATC 1 cut(s) 1027
BstMAI GTCTC 2 cut(s) 358, 670
BstMBI GATC 1 cut(s) 1024
BstMWI GCNNNNNNNGC 7 cut(s) 71, 720, 741, 945, 993, 1056, 1187
BstNI CCWGG 3 cut(s) 131, 573, 720
BstNSI RCATGY 2 cut(s) 557, 1104
BstSCI CCNGG 4 cut(s) 129, 571, 718, 912
BstSFI CTRYAG 2 cut(s) 72, 275
BstSLI GKGCMC 1 cut(s) 1016
BstUI CGCG 1 cut(s) 973
BstV1I GCAGC 2 cut(s) 58, 1062
BsuRI GGCC 6 cut(s) 378, 718, 723, 841, 863, 948
BtgI CCRYGG 1 cut(s) 724
BtrI CACGTC 1 cut(s) 711
BtsIMutI CAGTG 2 cut(s) 225, 792
BveI ACCTGC 2 cut(s) 741, 1186
Cac8I GCNNGC 5 cut(s) 686, 716, 843, 1044, 1048
CfoI GCGC 1 cut(s) 638
Cfr10I RCCGGY 3 cut(s) 247, 632, 650
Cfr13I GGNCC 5 cut(s) 215, 233, 396, 716, 916
Csp6I GTAC 3 cut(s) 162, 754, 1125
CspAI ACCGGT 2 cut(s) 247, 650
CspCI CAANNNNNGTGG 2 cut(s) 299, 334
CviAII CATG 6 cut(s) 208, 517, 554, 837, 1065, 1101
CviQI GTAC 3 cut(s) 162, 754, 1125
DdeI CTNAG 2 cut(s) 20, 355
DpnI GATC 1 cut(s) 1026
DpnII GATC 1 cut(s) 1024
EaeI YGGCCR 2 cut(s) 376, 946
Eam1104I CTCTTC 2 cut(s) 59, 552
EarI CTCTTC 2 cut(s) 59, 552
Eco130I CCWWGG 4 cut(s) 66, 102, 379, 745
Eco32I GATATC 1 cut(s) 85
Eco47I GGWCC 4 cut(s) 215, 233, 396, 916
EcoNI CCTNNNNNAGG 1 cut(s) 774
EcoO109I RGGNCCY 1 cut(s) 233
EcoRII CCWGG 3 cut(s) 129, 571, 718
EcoRV GATATC 1 cut(s) 85
EcoT14I CCWWGG 4 cut(s) 66, 102, 379, 745
ErhI CCWWGG 4 cut(s) 66, 102, 379, 745
FaeI CATG 6 cut(s) 211, 520, 557, 840, 1068, 1104
FatI CATG 6 cut(s) 207, 516, 553, 836, 1064, 1100
FauI CCCGC 2 cut(s) 707, 966
Fnu4HI GCNGC 2 cut(s) 72, 1051
Fsp4HI GCNGC 2 cut(s) 72, 1051
FspBI CTAG 1 cut(s) 746
GlaI GCGC 1 cut(s) 637
GluI GCNGC 2 cut(s) 72, 1051
HaeIII GGCC 6 cut(s) 378, 718, 723, 841, 863, 948
HapII CCGG 5 cut(s) 248, 284, 633, 651, 914
HhaI GCGC 1 cut(s) 638
Hin1II CATG 6 cut(s) 211, 520, 557, 840, 1068, 1104
Hin6I GCGC 1 cut(s) 636
HinP1I GCGC 1 cut(s) 636
HincII GTYRAC 1 cut(s) 615
HindII GTYRAC 1 cut(s) 615
HindIII AAGCTT 1 cut(s) 1040
HinfI GANTC 2 cut(s) 544, 597
HpaII CCGG 5 cut(s) 248, 284, 633, 651, 914
HphI GGTGA 5 cut(s) 622, 665, 686, 718, 840
Hpy166II GTNNAC 2 cut(s) 615, 1147
Hpy188I TCNGA 2 cut(s) 868, 1071
Hpy188III TCNNGA 6 cut(s) 88, 357, 482, 526, 700, 1000
Hpy8I GTNNAC 2 cut(s) 615, 1147
Hpy99I CGWCG 2 cut(s) 596, 973
HpyAV CCTTC 3 cut(s) 260, 266, 697
HpyCH4III ACNGT 3 cut(s) 184, 787, 1156
HpyCH4IV ACGT 3 cut(s) 540, 710, 900
HpyF10VI GCNNNNNNNGC 7 cut(s) 71, 720, 741, 945, 993, 1056, 1187
HpyF3I CTNAG 2 cut(s) 20, 355
HpySE526I ACGT 3 cut(s) 540, 710, 900
Hsp92II CATG 6 cut(s) 211, 520, 557, 840, 1068, 1104
HspAI GCGC 1 cut(s) 636
KpnI GGTACC 1 cut(s) 757
Kzo9I GATC 1 cut(s) 1024
LmnI GCTCC 2 cut(s) 98, 286
Lsp1109I GCAGC 2 cut(s) 58, 1062
LweI GCATC 1 cut(s) 331
MaeI CTAG 1 cut(s) 746
MaeII ACGT 3 cut(s) 540, 710, 900
MaeIII GTNAC 8 cut(s) 306, 541, 556, 706, 781, 901, 1085, 1156
MalI GATC 1 cut(s) 1026
MboI GATC 1 cut(s) 1024
MboII GAAGA 2 cut(s) 46, 539
MfeI CAATTG 1 cut(s) 387
MhlI GDGCHC 1 cut(s) 1016
MlsI TGGCCA 2 cut(s) 378, 948
MluCI AATT 7 cut(s) 387, 642, 690, 793, 888, 1080, 1093
MluNI TGGCCA 2 cut(s) 378, 948
MlyI GAGTC 1 cut(s) 538
MnlI CCTC 5 cut(s) 353, 770, 874, 874, 1027
Mox20I TGGCCA 2 cut(s) 378, 948
MscI TGGCCA 2 cut(s) 378, 948
MseI TTAA 8 cut(s) 138, 449, 455, 693, 831, 978, 1092, 1201
MslI CAYNNNNRTG 3 cut(s) 185, 890, 1132
Msp20I TGGCCA 2 cut(s) 378, 948
MspI CCGG 5 cut(s) 248, 284, 633, 651, 914
MspR9I CCNGG 4 cut(s) 131, 573, 720, 914
MunI CAATTG 1 cut(s) 387
Mva1269I GAATGC 1 cut(s) 1059
MvaI CCWGG 3 cut(s) 131, 573, 720
MvnI CGCG 1 cut(s) 973
MwoI GCNNNNNNNGC 7 cut(s) 71, 720, 741, 945, 993, 1056, 1187
NciI CCSGG 1 cut(s) 914
NdeII GATC 1 cut(s) 1024
NlaIII CATG 6 cut(s) 211, 520, 557, 840, 1068, 1104
NlaIV GGNNCC 7 cut(s) 100, 235, 271, 282, 755, 1015, 1120
NmuCI GTSAC 8 cut(s) 306, 541, 556, 706, 781, 901, 1085, 1156
NspI RCATGY 2 cut(s) 557, 1104
NspV TTCGAA 1 cut(s) 8
OliI CACNNNNGTG 1 cut(s) 185
PaqCI CACCTGC 1 cut(s) 1186
PciI ACATGT 1 cut(s) 553
PcsI WCGNNNNNNNCGW 1 cut(s) 573
PctI GAATGC 1 cut(s) 1059
PfeI GAWTC 1 cut(s) 597
PinAI ACCGGT 2 cut(s) 247, 650
PkrI GCNGC 2 cut(s) 73, 1052
PleI GAGTC 1 cut(s) 538
PpsI GAGTC 1 cut(s) 538
PpuMI RGGWCCY 1 cut(s) 233
PscI ACATGT 1 cut(s) 553
PshBI ATTAAT 2 cut(s) 138, 1092
Psp5II RGGWCCY 1 cut(s) 233
Psp6I CCWGG 3 cut(s) 129, 571, 718
PspGI CCWGG 3 cut(s) 129, 571, 718
PspN4I GGNNCC 7 cut(s) 100, 235, 271, 282, 755, 1015, 1120
PspPI GGNCC 5 cut(s) 215, 233, 396, 716, 916
PspPPI RGGWCCY 1 cut(s) 233
PstI CTGCAG 1 cut(s) 76
RsaI GTAC 3 cut(s) 163, 755, 1126
RsaNI GTAC 3 cut(s) 162, 754, 1125
RseI CAYNNNNRTG 3 cut(s) 185, 890, 1132
SaqAI TTAA 8 cut(s) 138, 449, 455, 693, 831, 978, 1092, 1201
SatI GCNGC 2 cut(s) 72, 1051
Sau3AI GATC 1 cut(s) 1024
Sau96I GGNCC 5 cut(s) 215, 233, 396, 716, 916
SchI GAGTC 1 cut(s) 538
ScrFI CCNGG 4 cut(s) 131, 573, 720, 914
SduI GDGCHC 1 cut(s) 1016
SfaNI GCATC 1 cut(s) 331
SfcI CTRYAG 2 cut(s) 72, 275
SfuI TTCGAA 1 cut(s) 8
SgrAI CRCCGGYG 1 cut(s) 632
SinI GGWCC 4 cut(s) 215, 233, 396, 916
SmiMI CAYNNNNRTG 3 cut(s) 185, 890, 1132
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
Sse9I AATT 7 cut(s) 387, 642, 690, 793, 888, 1080, 1093
SsiI CCGC 2 cut(s) 714, 973
SspMI CTAG 1 cut(s) 746
StyD4I CCNGG 4 cut(s) 129, 571, 718, 912
StyI CCWWGG 4 cut(s) 66, 102, 379, 745
TaaI ACNGT 3 cut(s) 184, 787, 1156
TaiI ACGT 3 cut(s) 543, 713, 903
TaqI TCGA 3 cut(s) 8, 699, 879
TasI AATT 7 cut(s) 387, 642, 690, 793, 888, 1080, 1093
TatI WGTACW 2 cut(s) 161, 1124
TfiI GAWTC 1 cut(s) 597
Tru1I TTAA 8 cut(s) 138, 449, 455, 693, 831, 978, 1092, 1201
Tru9I TTAA 8 cut(s) 138, 449, 455, 693, 831, 978, 1092, 1201
TscAI CASTG 2 cut(s) 225, 792
TseFI GTSAC 8 cut(s) 306, 541, 556, 706, 781, 901, 1085, 1156
TseI GCWGC 2 cut(s) 71, 1050
Tsp45I GTSAC 8 cut(s) 306, 541, 556, 706, 781, 901, 1085, 1156
TspDTI ATGAA 2 cut(s) 46, 533
TspGWI ACGGA 1 cut(s) 609
TspRI CASTG 2 cut(s) 225, 792
VpaK11BI GGWCC 4 cut(s) 215, 233, 396, 916
VspI ATTAAT 2 cut(s) 138, 1092
XagI CCTNNNNNAGG 1 cut(s) 774
XapI RAATTY 1 cut(s) 642
XceI RCATGY 2 cut(s) 557, 1104
XmaJI CCTAGG 1 cut(s) 745
XspI CTAG 1 cut(s) 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.