Rh2AG522700
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0015088
Physical Location & Seq
Forward (+)
74962933 .. 74964451
1519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG522700.1

Sequence Viewer

Length: 1200 bp
ATGGGTTCAAAATCTTTCCTTGGCACTTGTTTTTTATTCTTGGTAGCGTTCTCCTTAAAAACCAATGCTGCAGATTTTGATATCAAGAAATATGGAGCCAAGGCAGATGGCAGGACAGATGACAGCCAGGCCATCAATAATGCATGGAGAGACGCATGTGCAGCCGCGACTCCAAGCACGGTTGTGATAGCGAAGGGTAAATATTTGGTTGGTCCGGTCAGGTTTGGAGGACCCTGCAAGGCAGCGGTTAGCATTAGGGTTGAAGGAACCCTACAGGCTCCGGCTGAACCTGAAAAGCTCAAATCCCAGGATGGGTGGGTAGTTTTCCAAAATGTCGATAAGTTATCCGTGTTCGGAGGGGGCACTTTTGACGGCCAAGGAAAACTAGCTTGGTCCAAAAATAATTGTGCCAAAACTGGAAAGTGCAATTCACTTCCTATCAATATACGATTGACTGGGGTTACCAATTCACACATTCAACACATAACATCGTTGAACAGCAAGTTGTTTCACATGAACGTCATCAACTGCAAACACCTGACCCTCGAACACATCACCATCGATGCACCTCAAGAAAGTCTAAACACAGATGGAATCCATATTGGTCGGTCCTCAAATATCAATGTTACCAATGCAAACATCAAAACTGGGGATGATTGTGTCTCTATCGGTGACGGAAGCCAGCAAATAAATGTCGAGAAGGTGATATGCGGCCCTGGCCATGGCATTAGTATCGGTAGCCTAGGAAGATATCACAACGAGCAACCTGTTGAGGGAGTCACTGTGAGAAACTGCACCATTTCGAATACCTCAAATGGTGTTAGGATTAAAACCTGGCCAGCTTCTCCAAATGGTGTGGCCTCAAATTTGCATTTCGAGGACATTATTATGAACAACGTCAAAACCACCCCTGTGCTTATAGACCAAGGATATTGCCCATATGGTCAATGTCAAGCAAGGTTACCGTCTAAAGTCAAGATAACCAATGTGAGCTTCAAGAACATTAGGGGCACATCTCAAGACCCTGTGGCTGTGAAGCTCGTTTGCAGCAAAGGGATACCGTGCCAGAATGTGCAAGTTGCTGACATCAACTTGACATATAATGGGAACAAAGGAACTGCTACATCCGAATGTGCCAACGTGAAGCCTACCGTGTCAGGAAAAGTTAATCCACAAGTTTGTGCAAAGAACGTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

399

Amino Acids

42.48

Weight (kDa)

9.08

Isoelectric Point (pI)

26.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 27 - 70 1.7e-09 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 53 - 384 4.1e-104 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07850 AT3G14040
fragaria_vesca FvH4_6g41360
malus_domestica MD17G1110900.v1.1
prunus_persica Prupe.3G203200_v2.0.a1
pyrus_communis pycom17g10330
rosa_chinensis RchiOBHm_Chr2g0156901
rosa_laevigata RLG00000020951
rosa_multiflora Rmu_ssc0000408.1_g000002
rosa_roxburghii Rroxscaffold_2G00092600
rosa_rugosa Rorug02G0456900 Rorug02G0457000
rosa_samantha Rh2AG522700 Rh2BG536000 Rh2CG507600 Rh2DG545000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 167
AciI CCGC 3 cut(s) 165, 245, 711
AclI AACGTT 1 cut(s) 1191
AcoI YGGCCR 3 cut(s) 373, 718, 836
AcsI RAATTY 1 cut(s) 865
AfiI CCNNNNNNNGG 3 cut(s) 312, 722, 773
AgsI TTSAA 5 cut(s) 9, 263, 479, 496, 997
AjnI CCWGG 4 cut(s) 126, 306, 715, 833
AjuI GAANNNNNNNTTGG 4 cut(s) 373, 405, 977, 1009
AleI CACNNNNGTG 2 cut(s) 182, 911
AluBI AGCT 5 cut(s) 298, 389, 842, 993, 1039
AluI AGCT 5 cut(s) 298, 389, 842, 993, 1039
Alw26I GTCTC 2 cut(s) 144, 667
AoxI GGCC 6 cut(s) 129, 373, 712, 718, 836, 858
ApeKI GCWGC 4 cut(s) 68, 161, 242, 1047
ApoI RAATTY 1 cut(s) 865
AspA2I CCTAGG 1 cut(s) 742
AspS9I GGNCC 5 cut(s) 212, 230, 393, 609, 713
AsuHPI GGTGA 3 cut(s) 547, 683, 715
AsuII TTCGAA 1 cut(s) 803
AvaII GGWCC 4 cut(s) 212, 230, 393, 609
AvrII CCTAGG 1 cut(s) 742
BaeGI GKGCMC 2 cut(s) 365, 1013
BalI TGGCCA 2 cut(s) 720, 838
BarI GAAGNNNNNNTAC 2 cut(s) 255, 287
BbvI GCAGC 4 cut(s) 55, 173, 254, 1059
BccI CCATC 5 cut(s) 101, 140, 305, 566, 584
BceAI ACGGC 1 cut(s) 388
BcgI CGANNNNNNTGC 2 cut(s) 715, 749
BciT130I CCWGG 4 cut(s) 128, 308, 717, 835
BciVI GTATCC 1 cut(s) 1050
BcoDI GTCTC 2 cut(s) 144, 667
BfaI CTAG 2 cut(s) 386, 743
BfmI CTRYAG 2 cut(s) 69, 272
BfuI GTATCC 1 cut(s) 1050
BisI GCNGC 6 cut(s) 69, 162, 165, 243, 712, 1048
BlnI CCTAGG 1 cut(s) 742
BlsI GCNGC 6 cut(s) 70, 163, 166, 244, 713, 1049
Bme1390I CCNGG 4 cut(s) 128, 308, 717, 835
Bme18I GGWCC 4 cut(s) 212, 230, 393, 609
BmgT120I GGNCC 5 cut(s) 212, 230, 393, 609, 713
BmiI GGNNCC 4 cut(s) 97, 232, 268, 279
BmrFI CCNGG 4 cut(s) 128, 308, 717, 835
BmrI ACTGGG 2 cut(s) 465, 657
BmsI GCATC 1 cut(s) 553
BmuI ACTGGG 2 cut(s) 465, 657
Bpu14I TTCGAA 1 cut(s) 803
BpuEI CTTGAG 2 cut(s) 555, 1002
Bsa29I ATCGAT 1 cut(s) 561
BsaJI CCNNGG 8 cut(s) 19, 99, 306, 376, 715, 721, 742, 925
BsaWI WCCGGW 1 cut(s) 214
BsaXI ACNNNNNCTCC 2 cut(s) 768, 798
Bsc4I CCNNNNNNNGG 3 cut(s) 312, 722, 773
Bse1I ACTGG 3 cut(s) 421, 460, 652
BseBI CCWGG 4 cut(s) 128, 308, 717, 835
BseCI ATCGAT 1 cut(s) 561
BseDI CCNNGG 8 cut(s) 19, 99, 306, 376, 715, 721, 742, 925
BseGI GGATG 3 cut(s) 316, 658, 1124
BseLI CCNNNNNNNGG 3 cut(s) 312, 722, 773
BseNI ACTGG 3 cut(s) 421, 460, 652
BseSI GKGCMC 2 cut(s) 365, 1013
BseXI GCAGC 4 cut(s) 55, 173, 254, 1059
BsgI GTGCAG 2 cut(s) 180, 778
Bsh1236I CGCG 1 cut(s) 167
BshFI GGCC 6 cut(s) 131, 375, 714, 720, 838, 860
BshVI ATCGAT 1 cut(s) 561
BsiSI CCGG 2 cut(s) 215, 281
BslI CCNNNNNNNGG 3 cut(s) 312, 722, 773
BsmAI GTCTC 2 cut(s) 144, 667
BsmBI CGTCTC 1 cut(s) 144
BsnI GGCC 6 cut(s) 131, 375, 714, 720, 838, 860
Bsp119I TTCGAA 1 cut(s) 803
Bsp1286I GDGCHC 2 cut(s) 365, 1013
Bsp19I CCATGG 1 cut(s) 721
BspACI CCGC 3 cut(s) 165, 245, 711
BspANI GGCC 6 cut(s) 131, 375, 714, 720, 838, 860
BspDI ATCGAT 1 cut(s) 561
BspFNI CGCG 1 cut(s) 167
BspLI GGNNCC 4 cut(s) 97, 232, 268, 279
BspMAI CTGCAG 1 cut(s) 73
BspT104I TTCGAA 1 cut(s) 803
BsrI ACTGG 3 cut(s) 421, 460, 652
BssECI CCNNGG 8 cut(s) 19, 99, 306, 376, 715, 721, 742, 925
BssT1I CCWWGG 6 cut(s) 19, 99, 376, 721, 742, 925
Bst2UI CCWGG 4 cut(s) 128, 308, 717, 835
Bst4CI ACNGT 5 cut(s) 181, 784, 966, 1062, 1153
BstBI TTCGAA 1 cut(s) 803
BstC8I GCNNGC 2 cut(s) 683, 840
BstDSI CCRYGG 1 cut(s) 721
BstEII GGTNACC 2 cut(s) 460, 960
BstF5I GGATG 3 cut(s) 316, 658, 1124
BstFNI CGCG 1 cut(s) 167
BstMAI GTCTC 2 cut(s) 144, 667
BstMWI GCNNNNNNNGC 2 cut(s) 161, 717
BstNI CCWGG 4 cut(s) 128, 308, 717, 835
BstNSI RCATGY 1 cut(s) 159
BstPI GGTNACC 2 cut(s) 460, 960
BstSCI CCNGG 4 cut(s) 126, 306, 715, 833
BstSFI CTRYAG 2 cut(s) 69, 272
BstSLI GKGCMC 2 cut(s) 365, 1013
BstUI CGCG 1 cut(s) 167
BstV1I GCAGC 4 cut(s) 55, 173, 254, 1059
Bsu15I ATCGAT 1 cut(s) 561
BsuI GTATCC 1 cut(s) 1050
BsuRI GGCC 6 cut(s) 131, 375, 714, 720, 838, 860
BsuTUI ATCGAT 1 cut(s) 561
BtgI CCRYGG 1 cut(s) 721
BtsCI GGATG 3 cut(s) 316, 658, 1124
BtsIMutI CAGTG 1 cut(s) 780
Cac8I GCNNGC 2 cut(s) 683, 840
Cfr13I GGNCC 5 cut(s) 212, 230, 393, 609, 713
ClaI ATCGAT 1 cut(s) 561
CseI GACGC 1 cut(s) 161
CspCI CAANNNNNGTGG 2 cut(s) 837, 872
CviAII CATG 4 cut(s) 144, 156, 514, 722
EaeI YGGCCR 3 cut(s) 373, 718, 836
Eco130I CCWWGG 6 cut(s) 19, 99, 376, 721, 742, 925
Eco32I GATATC 2 cut(s) 82, 752
Eco47I GGWCC 4 cut(s) 212, 230, 393, 609
Eco91I GGTNACC 2 cut(s) 460, 960
EcoO109I RGGNCCY 1 cut(s) 230
EcoO65I GGTNACC 2 cut(s) 460, 960
EcoRII CCWGG 4 cut(s) 126, 306, 715, 833
EcoRV GATATC 2 cut(s) 82, 752
EcoT14I CCWWGG 6 cut(s) 19, 99, 376, 721, 742, 925
EcoT22I ATGCAT 1 cut(s) 145
ErhI CCWWGG 6 cut(s) 19, 99, 376, 721, 742, 925
Esp3I CGTCTC 1 cut(s) 144
FaeI CATG 4 cut(s) 147, 159, 517, 725
FatI CATG 4 cut(s) 143, 155, 513, 721
FauNDI CATATG 1 cut(s) 940
Fnu4HI GCNGC 6 cut(s) 69, 162, 165, 243, 712, 1048
FokI GGATG 3 cut(s) 323, 665, 1111
Fsp4HI GCNGC 6 cut(s) 69, 162, 165, 243, 712, 1048
FspBI CTAG 2 cut(s) 386, 743
GluI GCNGC 6 cut(s) 69, 162, 165, 243, 712, 1048
HaeIII GGCC 6 cut(s) 131, 375, 714, 720, 838, 860
HapII CCGG 2 cut(s) 215, 281
HgaI GACGC 1 cut(s) 161
Hin1II CATG 4 cut(s) 147, 159, 517, 725
HinfI GANTC 3 cut(s) 169, 594, 777
HpaII CCGG 2 cut(s) 215, 281
HphI GGTGA 3 cut(s) 547, 683, 715
Hpy188I TCNGA 2 cut(s) 356, 1129
Hpy188III TCNNGA 7 cut(s) 85, 572, 697, 976, 997, 1019, 1158
HpyAV CCTTC 3 cut(s) 187, 257, 694
HpyCH4III ACNGT 5 cut(s) 181, 784, 966, 1062, 1153
HpyCH4IV ACGT 4 cut(s) 519, 897, 1140, 1191
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 717
HpySE526I ACGT 4 cut(s) 519, 897, 1140, 1191
Hsp92II CATG 4 cut(s) 147, 159, 517, 725
LmnI GCTCC 2 cut(s) 95, 283
Lsp1109I GCAGC 4 cut(s) 55, 173, 254, 1059
LweI GCATC 1 cut(s) 553
MaeI CTAG 2 cut(s) 386, 743
MaeII ACGT 4 cut(s) 519, 897, 1140, 1191
MaeIII GTNAC 5 cut(s) 460, 625, 671, 778, 960
MboII GAAGA 1 cut(s) 759
MhlI GDGCHC 2 cut(s) 365, 1013
MlsI TGGCCA 2 cut(s) 720, 838
MluCI AATT 4 cut(s) 403, 427, 466, 865
MluNI TGGCCA 2 cut(s) 720, 838
MlyI GAGTC 2 cut(s) 163, 786
MnlI CCTC 9 cut(s) 221, 350, 554, 579, 622, 766, 820, 871, 871
Mox20I TGGCCA 2 cut(s) 720, 838
Mph1103I ATGCAT 1 cut(s) 145
MscI TGGCCA 2 cut(s) 720, 838
MseI TTAA 4 cut(s) 56, 828, 1167, 1198
MslI CAYNNNNRTG 4 cut(s) 182, 887, 911, 1129
Msp20I TGGCCA 2 cut(s) 720, 838
MspA1I CMGCKG 1 cut(s) 245
MspI CCGG 2 cut(s) 215, 281
MspR9I CCNGG 4 cut(s) 128, 308, 717, 835
MvaI CCWGG 4 cut(s) 128, 308, 717, 835
MvnI CGCG 1 cut(s) 167
MwoI GCNNNNNNNGC 2 cut(s) 161, 717
NcoI CCATGG 1 cut(s) 721
NdeI CATATG 1 cut(s) 940
NlaIII CATG 4 cut(s) 147, 159, 517, 725
NlaIV GGNNCC 4 cut(s) 97, 232, 268, 279
NmuCI GTSAC 2 cut(s) 671, 778
NsiI ATGCAT 1 cut(s) 145
NspI RCATGY 1 cut(s) 159
NspV TTCGAA 1 cut(s) 803
OliI CACNNNNGTG 2 cut(s) 182, 911
PfeI GAWTC 1 cut(s) 594
PkrI GCNGC 6 cut(s) 70, 163, 166, 244, 713, 1049
PleI GAGTC 2 cut(s) 163, 785
PpsI GAGTC 2 cut(s) 163, 785
PpuMI RGGWCCY 1 cut(s) 230
Psp1406I AACGTT 1 cut(s) 1191
Psp5II RGGWCCY 1 cut(s) 230
Psp6I CCWGG 4 cut(s) 126, 306, 715, 833
PspEI GGTNACC 2 cut(s) 460, 960
PspGI CCWGG 4 cut(s) 126, 306, 715, 833
PspN4I GGNNCC 4 cut(s) 97, 232, 268, 279
PspPI GGNCC 5 cut(s) 212, 230, 393, 609, 713
PspPPI RGGWCCY 1 cut(s) 230
PstI CTGCAG 1 cut(s) 73
RseI CAYNNNNRTG 4 cut(s) 182, 887, 911, 1129
SaqAI TTAA 4 cut(s) 56, 828, 1167, 1198
SatI GCNGC 6 cut(s) 69, 162, 165, 243, 712, 1048
Sau96I GGNCC 5 cut(s) 212, 230, 393, 609, 713
SchI GAGTC 2 cut(s) 163, 786
ScrFI CCNGG 4 cut(s) 128, 308, 717, 835
SduI GDGCHC 2 cut(s) 365, 1013
SfaNI GCATC 1 cut(s) 553
SfcI CTRYAG 2 cut(s) 69, 272
SfuI TTCGAA 1 cut(s) 803
SinI GGWCC 4 cut(s) 212, 230, 393, 609
SmiMI CAYNNNNRTG 4 cut(s) 182, 887, 911, 1129
SmlI CTYRAG 2 cut(s) 570, 1017
SmoI CTYRAG 2 cut(s) 570, 1017
Sse9I AATT 4 cut(s) 403, 427, 466, 865
SsiI CCGC 3 cut(s) 165, 245, 711
SspI AATATT 1 cut(s) 203
SspMI CTAG 2 cut(s) 386, 743
StyD4I CCNGG 4 cut(s) 126, 306, 715, 833
StyI CCWWGG 6 cut(s) 19, 99, 376, 721, 742, 925
TaaI ACNGT 5 cut(s) 181, 784, 966, 1062, 1153
TaiI ACGT 4 cut(s) 522, 900, 1143, 1194
TaqI TCGA 6 cut(s) 336, 546, 561, 696, 803, 876
TaqII GACCGA 1 cut(s) 597
TasI AATT 4 cut(s) 403, 427, 466, 865
TauI GCSGC 2 cut(s) 167, 714
TfiI GAWTC 1 cut(s) 594
Tru1I TTAA 4 cut(s) 56, 828, 1167, 1198
Tru9I TTAA 4 cut(s) 56, 828, 1167, 1198
TscAI CASTG 1 cut(s) 787
TseFI GTSAC 2 cut(s) 671, 778
TseI GCWGC 4 cut(s) 68, 161, 242, 1047
Tsp45I GTSAC 2 cut(s) 671, 778
TspDTI ATGAA 2 cut(s) 530, 905
TspGWI ACGGA 2 cut(s) 337, 690
TspRI CASTG 1 cut(s) 787
VpaK11BI GGWCC 4 cut(s) 212, 230, 393, 609
XapI RAATTY 1 cut(s) 865
XceI RCATGY 1 cut(s) 159
XmaJI CCTAGG 1 cut(s) 742
XspI CTAG 2 cut(s) 386, 743
Zsp2I ATGCAT 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.