Rh2BG536000
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0015088
Physical Location & Seq
Forward (+)
74471580 .. 74474396
2817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG536000.1

Sequence Viewer

Length: 1116 bp
ATGTACAAACAATGTGACTACACAACGTATGCATTATTATCTCAGGCCATCAATAATGCATGGAGAGACGCATGTGCAGCCGCGACTCCAAGCACGGTTGTGATAGCGAAGGGTAAATATTTGGTTGGTCCGGTCAGGTTTGGAGGACCCTGCAAGGCAGCGGTTAGCATTAGGGTTGAAGGAACCCTACAGGCTCCGGCTGAACCTGAAAAACTCAAATCCCAGGATGGGTGGGTAGTTTTCCAAAATGTCGACAAGTTATCCGTGTTCGGAGGGGGCACTTTTGACGGCCAAGGAAAACTAGCTTGGTCCAAAAATAATTGTGCCAAAACTGGAAAGTGCAATTCACTTCCTATCAATATACGATTGACTGGGGTTACCAATTCACACATTCAACACATAACATCGTTGAACAGCAAGTTGTTTCACATGAACGTCATCAACTGCAAACACCTGACCCTCGAACACATCACCATCGATGCACCTCAAGAAAGTCTAAACACAGATGGAATCCATATTGGTCGGTCCTCAAATATCAATGTTACCAATGCAAACATCAAAACTGGGGATGATTGTGTCTCTATCGGTGACGGAAGCCAGCAAATAAATGTCGAGAAGGTGATATGCGGCCCTGGCCATGGCATTAGTATCGGTAGCCTAGGAAGATATCACAACGAGCAACCTGTTGAGGGAGTCACTGTGAGAAACTGCACCGTTTCGAATACTTCAAATGGTGTAAGGATTAAAACCTGGCCAGCTTCTCCAAATGGTGTGGCCTCAAATTTGCATTTCGAGGACATTATTATGAACAACGTCAAAACCACCCCTGTGCTTATAGACCAAGGATATTGCCCATATGGTCAATGTCAAGCAAGGTTACCGTCTAAAGTCAAGATAACCAATGTGAGCTTCAAGAACATTAGGGGCACATCTCAAGACCCTGTGGCTGTGAAGCTCGTTTGCAGCAAAGGGATACCGTGCCAGAATGTGCAAGTTGCTGACATCAACTTGACATATAATGGGAACAAAGGAACTGCTACATCCGAATGTGCCAACGTGAAGCCTACCGTATCAGGAAAAGTTAATCCACAAGTTTGTGCAAAGAACGTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

371

Amino Acids

39.63

Weight (kDa)

9.03

Isoelectric Point (pI)

31.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 25 - 356 3.4e-104 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07850 AT3G14040
fragaria_vesca FvH4_6g41360
malus_domestica MD17G1110900.v1.1
prunus_persica Prupe.3G203200_v2.0.a1
pyrus_communis pycom17g10330
rosa_chinensis RchiOBHm_Chr2g0156901
rosa_laevigata RLG00000020951
rosa_multiflora Rmu_ssc0000408.1_g000002
rosa_roxburghii Rroxscaffold_2G00092600
rosa_rugosa Rorug02G0456900 Rorug02G0457000
rosa_samantha Rh2AG522700 Rh2BG536000 Rh2CG507600 Rh2DG545000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 252
AccII CGCG 1 cut(s) 83
AciI CCGC 3 cut(s) 81, 161, 627
AclI AACGTT 1 cut(s) 1107
AcoI YGGCCR 3 cut(s) 289, 634, 752
AcsI RAATTY 1 cut(s) 781
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 3 cut(s) 228, 638, 689
AgsI TTSAA 5 cut(s) 179, 395, 412, 729, 913
AjnI CCWGG 3 cut(s) 222, 631, 749
AjuI GAANNNNNNNTTGG 4 cut(s) 289, 321, 893, 925
AleI CACNNNNGTG 2 cut(s) 98, 827
AluBI AGCT 4 cut(s) 305, 758, 909, 955
AluI AGCT 4 cut(s) 305, 758, 909, 955
Alw26I GTCTC 2 cut(s) 60, 583
AoxI GGCC 6 cut(s) 45, 289, 628, 634, 752, 774
ApeKI GCWGC 3 cut(s) 77, 158, 963
ApoI RAATTY 1 cut(s) 781
AspA2I CCTAGG 1 cut(s) 658
AspS9I GGNCC 5 cut(s) 128, 146, 309, 525, 629
AsuHPI GGTGA 3 cut(s) 463, 599, 631
AsuII TTCGAA 1 cut(s) 719
AvaII GGWCC 4 cut(s) 128, 146, 309, 525
AvrII CCTAGG 1 cut(s) 658
BaeGI GKGCMC 2 cut(s) 281, 929
BalI TGGCCA 2 cut(s) 636, 754
BarI GAAGNNNNNNTAC 2 cut(s) 171, 203
BbvI GCAGC 3 cut(s) 89, 170, 975
BccI CCATC 4 cut(s) 56, 221, 482, 500
BceAI ACGGC 1 cut(s) 304
BcgI CGANNNNNNTGC 2 cut(s) 631, 665
BciT130I CCWGG 3 cut(s) 224, 633, 751
BciVI GTATCC 1 cut(s) 966
BcoDI GTCTC 2 cut(s) 60, 583
BfaI CTAG 2 cut(s) 302, 659
BfmI CTRYAG 1 cut(s) 188
BfuI GTATCC 1 cut(s) 966
BisI GCNGC 5 cut(s) 78, 81, 159, 628, 964
BlnI CCTAGG 1 cut(s) 658
BlsI GCNGC 5 cut(s) 79, 82, 160, 629, 965
Bme1390I CCNGG 3 cut(s) 224, 633, 751
Bme18I GGWCC 4 cut(s) 128, 146, 309, 525
BmgT120I GGNCC 5 cut(s) 128, 146, 309, 525, 629
BmiI GGNNCC 3 cut(s) 148, 184, 195
BmrFI CCNGG 3 cut(s) 224, 633, 751
BmrI ACTGGG 2 cut(s) 381, 573
BmsI GCATC 1 cut(s) 469
BmuI ACTGGG 2 cut(s) 381, 573
Bpu14I TTCGAA 1 cut(s) 719
BpuEI CTTGAG 2 cut(s) 471, 918
Bsa29I ATCGAT 1 cut(s) 477
BsaJI CCNNGG 6 cut(s) 222, 292, 631, 637, 658, 841
BsaWI WCCGGW 1 cut(s) 130
BsaXI ACNNNNNCTCC 2 cut(s) 684, 714
Bsc4I CCNNNNNNNGG 3 cut(s) 228, 638, 689
Bse1I ACTGG 3 cut(s) 337, 376, 568
BseBI CCWGG 3 cut(s) 224, 633, 751
BseCI ATCGAT 1 cut(s) 477
BseDI CCNNGG 6 cut(s) 222, 292, 631, 637, 658, 841
BseGI GGATG 3 cut(s) 232, 574, 1040
BseLI CCNNNNNNNGG 3 cut(s) 228, 638, 689
BseMII CTCAG 1 cut(s) 56
BseNI ACTGG 3 cut(s) 337, 376, 568
BseSI GKGCMC 2 cut(s) 281, 929
BseXI GCAGC 3 cut(s) 89, 170, 975
BsgI GTGCAG 2 cut(s) 96, 694
Bsh1236I CGCG 1 cut(s) 83
BshFI GGCC 6 cut(s) 47, 291, 630, 636, 754, 776
BshVI ATCGAT 1 cut(s) 477
BsiSI CCGG 2 cut(s) 131, 197
BslI CCNNNNNNNGG 3 cut(s) 228, 638, 689
BsmAI GTCTC 2 cut(s) 60, 583
BsmBI CGTCTC 1 cut(s) 60
BsnI GGCC 6 cut(s) 47, 291, 630, 636, 754, 776
Bsp119I TTCGAA 1 cut(s) 719
Bsp1286I GDGCHC 2 cut(s) 281, 929
Bsp1407I TGTACA 1 cut(s) 3
Bsp19I CCATGG 1 cut(s) 637
BspACI CCGC 3 cut(s) 81, 161, 627
BspANI GGCC 6 cut(s) 47, 291, 630, 636, 754, 776
BspCNI CTCAG 1 cut(s) 55
BspDI ATCGAT 1 cut(s) 477
BspFNI CGCG 1 cut(s) 83
BspLI GGNNCC 3 cut(s) 148, 184, 195
BspT104I TTCGAA 1 cut(s) 719
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 3 cut(s) 337, 376, 568
BssECI CCNNGG 6 cut(s) 222, 292, 631, 637, 658, 841
BssT1I CCWWGG 4 cut(s) 292, 637, 658, 841
Bst2UI CCWGG 3 cut(s) 224, 633, 751
Bst4CI ACNGT 6 cut(s) 97, 700, 715, 882, 978, 1069
BstAUI TGTACA 1 cut(s) 3
BstBI TTCGAA 1 cut(s) 719
BstC8I GCNNGC 2 cut(s) 599, 756
BstDEI CTNAG 1 cut(s) 42
BstDSI CCRYGG 1 cut(s) 637
BstEII GGTNACC 2 cut(s) 376, 876
BstF5I GGATG 3 cut(s) 232, 574, 1040
BstFNI CGCG 1 cut(s) 83
BstMAI GTCTC 2 cut(s) 60, 583
BstMWI GCNNNNNNNGC 2 cut(s) 77, 633
BstNI CCWGG 3 cut(s) 224, 633, 751
BstNSI RCATGY 1 cut(s) 75
BstPI GGTNACC 2 cut(s) 376, 876
BstSCI CCNGG 3 cut(s) 222, 631, 749
BstSFI CTRYAG 1 cut(s) 188
BstSLI GKGCMC 2 cut(s) 281, 929
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 3 cut(s) 89, 170, 975
Bsu15I ATCGAT 1 cut(s) 477
BsuI GTATCC 1 cut(s) 966
BsuRI GGCC 6 cut(s) 47, 291, 630, 636, 754, 776
BsuTUI ATCGAT 1 cut(s) 477
BtgI CCRYGG 1 cut(s) 637
BtsCI GGATG 3 cut(s) 232, 574, 1040
BtsIMutI CAGTG 1 cut(s) 696
Cac8I GCNNGC 2 cut(s) 599, 756
Cfr13I GGNCC 5 cut(s) 128, 146, 309, 525, 629
ClaI ATCGAT 1 cut(s) 477
CseI GACGC 1 cut(s) 77
Csp6I GTAC 1 cut(s) 4
CspCI CAANNNNNGTGG 2 cut(s) 753, 788
CviAII CATG 4 cut(s) 60, 72, 430, 638
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 1 cut(s) 42
EaeI YGGCCR 3 cut(s) 289, 634, 752
Eco130I CCWWGG 4 cut(s) 292, 637, 658, 841
Eco32I GATATC 1 cut(s) 668
Eco47I GGWCC 4 cut(s) 128, 146, 309, 525
Eco91I GGTNACC 2 cut(s) 376, 876
EcoO109I RGGNCCY 1 cut(s) 146
EcoO65I GGTNACC 2 cut(s) 376, 876
EcoRII CCWGG 3 cut(s) 222, 631, 749
EcoRV GATATC 1 cut(s) 668
EcoT14I CCWWGG 4 cut(s) 292, 637, 658, 841
EcoT22I ATGCAT 2 cut(s) 34, 61
ErhI CCWWGG 4 cut(s) 292, 637, 658, 841
Esp3I CGTCTC 1 cut(s) 60
FaeI CATG 4 cut(s) 63, 75, 433, 641
FatI CATG 4 cut(s) 59, 71, 429, 637
FauNDI CATATG 1 cut(s) 856
FblI GTMKAC 1 cut(s) 252
Fnu4HI GCNGC 5 cut(s) 78, 81, 159, 628, 964
FokI GGATG 3 cut(s) 239, 581, 1027
Fsp4HI GCNGC 5 cut(s) 78, 81, 159, 628, 964
FspBI CTAG 2 cut(s) 302, 659
GluI GCNGC 5 cut(s) 78, 81, 159, 628, 964
HaeIII GGCC 6 cut(s) 47, 291, 630, 636, 754, 776
HapII CCGG 2 cut(s) 131, 197
HgaI GACGC 1 cut(s) 77
Hin1II CATG 4 cut(s) 63, 75, 433, 641
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 3 cut(s) 85, 510, 693
HpaII CCGG 2 cut(s) 131, 197
HphI GGTGA 3 cut(s) 463, 599, 631
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 2 cut(s) 272, 1045
Hpy188III TCNNGA 6 cut(s) 488, 613, 892, 913, 935, 1074
Hpy8I GTNNAC 1 cut(s) 253
HpyAV CCTTC 3 cut(s) 103, 173, 610
HpyCH4III ACNGT 6 cut(s) 97, 700, 715, 882, 978, 1069
HpyCH4IV ACGT 5 cut(s) 26, 435, 813, 1056, 1107
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 633
HpyF3I CTNAG 1 cut(s) 42
HpySE526I ACGT 5 cut(s) 26, 435, 813, 1056, 1107
Hsp92II CATG 4 cut(s) 63, 75, 433, 641
LmnI GCTCC 1 cut(s) 199
Lsp1109I GCAGC 3 cut(s) 89, 170, 975
LweI GCATC 1 cut(s) 469
MaeI CTAG 2 cut(s) 302, 659
MaeII ACGT 5 cut(s) 26, 435, 813, 1056, 1107
MaeIII GTNAC 6 cut(s) 14, 376, 541, 587, 694, 876
MboII GAAGA 1 cut(s) 675
MhlI GDGCHC 2 cut(s) 281, 929
MlsI TGGCCA 2 cut(s) 636, 754
MluCI AATT 4 cut(s) 319, 343, 382, 781
MluNI TGGCCA 2 cut(s) 636, 754
MlyI GAGTC 2 cut(s) 79, 702
MnlI CCTC 8 cut(s) 137, 266, 470, 495, 538, 682, 787, 787
Mox20I TGGCCA 2 cut(s) 636, 754
Mph1103I ATGCAT 2 cut(s) 34, 61
MscI TGGCCA 2 cut(s) 636, 754
MseI TTAA 3 cut(s) 744, 1083, 1114
MslI CAYNNNNRTG 4 cut(s) 98, 803, 827, 1045
Msp20I TGGCCA 2 cut(s) 636, 754
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 2 cut(s) 131, 197
MspR9I CCNGG 3 cut(s) 224, 633, 751
MvaI CCWGG 3 cut(s) 224, 633, 751
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 2 cut(s) 77, 633
NcoI CCATGG 1 cut(s) 637
NdeI CATATG 1 cut(s) 856
NlaIII CATG 4 cut(s) 63, 75, 433, 641
NlaIV GGNNCC 3 cut(s) 148, 184, 195
NmuCI GTSAC 3 cut(s) 14, 587, 694
NsiI ATGCAT 2 cut(s) 34, 61
NspI RCATGY 1 cut(s) 75
NspV TTCGAA 1 cut(s) 719
OliI CACNNNNGTG 2 cut(s) 98, 827
PfeI GAWTC 1 cut(s) 510
PkrI GCNGC 5 cut(s) 79, 82, 160, 629, 965
PleI GAGTC 2 cut(s) 79, 701
PpsI GAGTC 2 cut(s) 79, 701
PpuMI RGGWCCY 1 cut(s) 146
Psp1406I AACGTT 1 cut(s) 1107
Psp5II RGGWCCY 1 cut(s) 146
Psp6I CCWGG 3 cut(s) 222, 631, 749
PspEI GGTNACC 2 cut(s) 376, 876
PspGI CCWGG 3 cut(s) 222, 631, 749
PspN4I GGNNCC 3 cut(s) 148, 184, 195
PspPI GGNCC 5 cut(s) 128, 146, 309, 525, 629
PspPPI RGGWCCY 1 cut(s) 146
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 4 cut(s) 98, 803, 827, 1045
SalI GTCGAC 1 cut(s) 251
SaqAI TTAA 3 cut(s) 744, 1083, 1114
SatI GCNGC 5 cut(s) 78, 81, 159, 628, 964
Sau96I GGNCC 5 cut(s) 128, 146, 309, 525, 629
SchI GAGTC 2 cut(s) 79, 702
ScrFI CCNGG 3 cut(s) 224, 633, 751
SduI GDGCHC 2 cut(s) 281, 929
SfaNI GCATC 1 cut(s) 469
SfcI CTRYAG 1 cut(s) 188
SfuI TTCGAA 1 cut(s) 719
SinI GGWCC 4 cut(s) 128, 146, 309, 525
SmiMI CAYNNNNRTG 4 cut(s) 98, 803, 827, 1045
SmlI CTYRAG 2 cut(s) 486, 933
SmoI CTYRAG 2 cut(s) 486, 933
Sse9I AATT 4 cut(s) 319, 343, 382, 781
SsiI CCGC 3 cut(s) 81, 161, 627
SspI AATATT 1 cut(s) 119
SspMI CTAG 2 cut(s) 302, 659
StyD4I CCNGG 3 cut(s) 222, 631, 749
StyI CCWWGG 4 cut(s) 292, 637, 658, 841
TaaI ACNGT 6 cut(s) 97, 700, 715, 882, 978, 1069
TaiI ACGT 5 cut(s) 29, 438, 816, 1059, 1110
TaqI TCGA 6 cut(s) 252, 462, 477, 612, 719, 792
TaqII GACCGA 1 cut(s) 513
TasI AATT 4 cut(s) 319, 343, 382, 781
TatI WGTACW 1 cut(s) 3
TauI GCSGC 2 cut(s) 83, 630
TfiI GAWTC 1 cut(s) 510
Tru1I TTAA 3 cut(s) 744, 1083, 1114
Tru9I TTAA 3 cut(s) 744, 1083, 1114
TscAI CASTG 1 cut(s) 703
TseFI GTSAC 3 cut(s) 14, 587, 694
TseI GCWGC 3 cut(s) 77, 158, 963
Tsp45I GTSAC 3 cut(s) 14, 587, 694
TspDTI ATGAA 2 cut(s) 446, 821
TspGWI ACGGA 2 cut(s) 253, 606
TspRI CASTG 1 cut(s) 703
VpaK11BI GGWCC 4 cut(s) 128, 146, 309, 525
XapI RAATTY 1 cut(s) 781
XceI RCATGY 1 cut(s) 75
XmaJI CCTAGG 1 cut(s) 658
XmiI GTMKAC 1 cut(s) 252
XspI CTAG 2 cut(s) 302, 659
Zsp2I ATGCAT 2 cut(s) 34, 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.