Prupe.3G203200_v2.0.a1
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0015088
Physical Location & Seq
Forward (+)
21085564 .. 21087309
1746 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G203200.1

Sequence Viewer

Length: 1203 bp
ATGGGTTCAAAATTTATTCTTGGAACTACATTCTTTTTGTTCTTGATATCATTTTCCATAAAAGCCAGAGCTGCAGATTTTGATATCAACAAGTATGGAGCCAAGGCTGACGGCAAGACAGATGATAGCCGGGCTATTAATAGTGCTTGGAAAGAAGCATGCGCATCCACAACTCCGAGCACGGTTGTGATAGCAAAAGGAAACTACTTGGCTGGTCCAGTGAAGTTTCAAGGGCCCTGCAAAGCACCGGTTAGCATTCGGGTTGAAGGAACCCTGCAGGCTCCGGCAGAGCCAGAAAAGCTCAAGTCACAAGATGGTTGGGTTGTTTTCCGAAATATTGATGGGTTAACAGTCTCAGGAGGTGGCACTTTTGATGGCCAAGGATCAATAGCTTGGTCCAAAAATGATTGTGCCAAAACTGGAAAATGCAACTCACTCCCTATAAACATAAGATTCACAGGGCTTACCAATTCACACATTCAAAACATAACATCTTTGAACAGCAAGCTGTTCCACATAAACGTCTTGAACTGCAAAAACGTGACACTTCAACATGTGATCATCACCGCCCCTGGTGAAAGCCTAAACACAGATGGAATCCACATTGGCCGGTCATCGAACATCAACATCACCGGCGCAGAGATCAAAACCGGCGATGATTGCGTCTCTCTTGGCGATGGAAGCCAGCAAATAAACATTGAAAAGGTGAAGTGCGGGCCGGGCCACGGTATCAGCATTGGGAGCCTAGGCAGGTACCATGATGAACAACCTGTCACAGGAGTCACAGTGAGAAACTGCACCATATCAAACACTTCAAATGGCGTGAGGGTCAAGACATGGCCAGCTTCTCCAAACGGTGTGGCCTCAGATTTGCACTTTGAGGACATAATTATGGAAAATGTGAAGACCTCCCCTGTTCTTATAGACCAAGAATACTGTCCAAATGGCCAATGCCAAGCAAAAATCCCGTCGAAAGTTAAGATCAGCAATGTGAGCTTCAAGAACATTAGGGGCACTTCTGCTGATCCTGTTGTTGTGAAGCTTGCTTGTAGCAAAGGCATTCCTTGCCAGAATGTGCAAATTAGTGACATCCATTTGACATACAATGGGAAAAATGGTGCTGCCACATCTGTATGTACTAACGTCAAGCCCACTATGACTGGCCAAATTTTTCCACCTGCTTGTGCAAAGACTGCAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

401

Amino Acids

42.38

Weight (kDa)

8.92

Isoelectric Point (pI)

36.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07850 AT3G14040
fragaria_vesca FvH4_6g41360
malus_domestica MD17G1110900.v1.1
prunus_persica Prupe.3G203200_v2.0.a1
pyrus_communis pycom17g10330
rosa_chinensis RchiOBHm_Chr2g0156901
rosa_laevigata RLG00000020951
rosa_multiflora Rmu_ssc0000408.1_g000002
rosa_roxburghii Rroxscaffold_2G00092600
rosa_rugosa Rorug02G0456900 Rorug02G0457000
rosa_samantha Rh2AG522700 Rh2BG536000 Rh2CG507600 Rh2DG545000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1186
Acc16I TGCGCA 1 cut(s) 163
Acc36I ACCTGC 2 cut(s) 741, 1186
Acc65I GGTACC 1 cut(s) 753
AccB1I GGYRCC 1 cut(s) 753
AciI CCGC 2 cut(s) 567, 714
AclWI GGATC 2 cut(s) 391, 1019
AcoI YGGCCR 5 cut(s) 376, 607, 839, 946, 1162
AcsI RAATTY 2 cut(s) 11, 1167
AfaI GTAC 2 cut(s) 755, 1138
AfiI CCNNNNNNNGG 2 cut(s) 725, 776
AflIII ACRYGT 1 cut(s) 553
AgeI ACCGGT 1 cut(s) 247
AjnI CCWGG 1 cut(s) 571
AleI CACNNNNGTG 1 cut(s) 185
AluBI AGCT 8 cut(s) 71, 301, 392, 508, 845, 996, 1042, 1199
AluI AGCT 8 cut(s) 71, 301, 392, 508, 845, 996, 1042, 1199
Alw21I GWGCWC 1 cut(s) 182
Alw26I GTCTC 2 cut(s) 358, 670
AlwI GGATC 2 cut(s) 391, 1019
AoxI GGCC 9 cut(s) 233, 376, 607, 716, 721, 839, 861, 946, 1162
ApaI GGGCCC 1 cut(s) 237
ApeKI GCWGC 3 cut(s) 71, 1121, 1196
ApoI RAATTY 2 cut(s) 11, 1167
ArsI GACNNNNNNTTYG 2 cut(s) 953, 985
AseI ATTAAT 1 cut(s) 138
AsiGI ACCGGT 1 cut(s) 247
Asp718I GGTACC 1 cut(s) 753
AspA2I CCTAGG 1 cut(s) 745
AspLEI GCGC 2 cut(s) 164, 638
AspS9I GGNCC 6 cut(s) 215, 233, 234, 396, 716, 721
AsuC2I CCSGG 2 cut(s) 131, 720
AsuHPI GGTGA 4 cut(s) 556, 587, 622, 718
AvaII GGWCC 2 cut(s) 215, 396
AvrII CCTAGG 1 cut(s) 745
BaeGI GKGCMC 2 cut(s) 237, 1016
BalI TGGCCA 4 cut(s) 378, 841, 948, 1164
BanI GGYRCC 1 cut(s) 753
BanII GRGCYC 1 cut(s) 237
BbsI GAAGAC 1 cut(s) 911
Bbv12I GWGCWC 1 cut(s) 182
BbvI GCAGC 2 cut(s) 58, 1108
BccI CCATC 5 cut(s) 308, 335, 368, 587, 671
BceAI ACGGC 1 cut(s) 127
BciT130I CCWGG 1 cut(s) 573
BclI TGATCA 1 cut(s) 558
BcnI CCSGG 2 cut(s) 131, 720
BcoDI GTCTC 2 cut(s) 358, 670
BfaI CTAG 1 cut(s) 746
BfmI CTRYAG 3 cut(s) 72, 275, 1194
BfuAI ACCTGC 2 cut(s) 741, 1186
BisI GCNGC 3 cut(s) 72, 1122, 1197
BlnI CCTAGG 1 cut(s) 745
BlsI GCNGC 3 cut(s) 73, 1123, 1198
Bme1390I CCNGG 3 cut(s) 131, 573, 720
Bme18I GGWCC 2 cut(s) 215, 396
BmgT120I GGNCC 6 cut(s) 215, 233, 234, 396, 716, 721
BmiI GGNNCC 6 cut(s) 100, 235, 271, 282, 743, 755
BmrFI CCNGG 3 cut(s) 131, 573, 720
BmsI GCATC 1 cut(s) 173
BpiI GAAGAC 1 cut(s) 911
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 2 cut(s) 131, 720
BsaJI CCNNGG 5 cut(s) 102, 379, 571, 724, 745
BsaWI WCCGGW 1 cut(s) 247
BsaXI ACNNNNNCTCC 2 cut(s) 771, 801
Bsc4I CCNNNNNNNGG 2 cut(s) 725, 776
Bse118I RCCGGY 4 cut(s) 247, 609, 632, 650
Bse1I ACTGG 3 cut(s) 218, 424, 1165
Bse3DI GCAATG 1 cut(s) 994
BseBI CCWGG 1 cut(s) 573
BseDI CCNNGG 5 cut(s) 102, 379, 571, 724, 745
BseGI GGATG 2 cut(s) 164, 1089
BseLI CCNNNNNNNGG 2 cut(s) 725, 776
BseMI GCAATG 1 cut(s) 994
BseMII CTCAG 2 cut(s) 369, 879
BseNI ACTGG 3 cut(s) 218, 424, 1165
BseSI GKGCMC 2 cut(s) 237, 1016
BseXI GCAGC 2 cut(s) 58, 1108
BsgI GTGCAG 1 cut(s) 781
BshFI GGCC 9 cut(s) 235, 378, 609, 718, 723, 841, 863, 948, 1164
BshNI GGYRCC 1 cut(s) 753
BshTI ACCGGT 1 cut(s) 247
BsiHKAI GWGCWC 1 cut(s) 182
BsiSI CCGG 7 cut(s) 130, 248, 284, 610, 633, 651, 719
BslI CCNNNNNNNGG 2 cut(s) 725, 776
BsmAI GTCTC 2 cut(s) 358, 670
BsmBI CGTCTC 1 cut(s) 670
BsmI GAATGC 2 cut(s) 255, 1059
BsnI GGCC 9 cut(s) 235, 378, 609, 718, 723, 841, 863, 948, 1164
Bsp120I GGGCCC 1 cut(s) 233
Bsp1286I GDGCHC 3 cut(s) 182, 237, 1016
Bsp143I GATC 5 cut(s) 383, 558, 642, 981, 1024
BspACI CCGC 2 cut(s) 567, 714
BspANI GGCC 9 cut(s) 235, 378, 609, 718, 723, 841, 863, 948, 1164
BspCNI CTCAG 2 cut(s) 368, 878
BspLI GGNNCC 6 cut(s) 100, 235, 271, 282, 743, 755
BspMAI CTGCAG 3 cut(s) 76, 279, 1198
BspMI ACCTGC 2 cut(s) 741, 1186
BspPI GGATC 2 cut(s) 391, 1019
BspT107I GGYRCC 1 cut(s) 753
BsrDI GCAATG 1 cut(s) 994
BsrFI RCCGGY 4 cut(s) 247, 609, 632, 650
BsrI ACTGG 3 cut(s) 218, 424, 1165
BssAI RCCGGY 4 cut(s) 247, 609, 632, 650
BssECI CCNNGG 5 cut(s) 102, 379, 571, 724, 745
BssMI GATC 5 cut(s) 383, 558, 642, 981, 1024
BssT1I CCWWGG 3 cut(s) 102, 379, 745
Bst2UI CCWGG 1 cut(s) 573
Bst4CI ACNGT 6 cut(s) 184, 352, 728, 787, 857, 938
BstAPI GCANNNNNTGC 2 cut(s) 1065, 1193
BstC8I GCNNGC 7 cut(s) 160, 279, 506, 686, 716, 843, 1044
BstDEI CTNAG 2 cut(s) 355, 865
BstDSI CCRYGG 1 cut(s) 724
BstENI CCTNNNNNAGG 1 cut(s) 774
BstF5I GGATG 2 cut(s) 164, 1089
BstHHI GCGC 2 cut(s) 164, 638
BstKTI GATC 5 cut(s) 386, 561, 645, 984, 1027
BstMAI GTCTC 2 cut(s) 358, 670
BstMBI GATC 5 cut(s) 383, 558, 642, 981, 1024
BstMWI GCNNNNNNNGC 9 cut(s) 71, 298, 660, 681, 720, 741, 993, 1065, 1193
BstNI CCWGG 1 cut(s) 573
BstNSI RCATGY 2 cut(s) 162, 557
BstSCI CCNGG 3 cut(s) 129, 571, 718
BstSFI CTRYAG 3 cut(s) 72, 275, 1194
BstSLI GKGCMC 2 cut(s) 237, 1016
BstV1I GCAGC 2 cut(s) 58, 1108
BstV2I GAAGAC 1 cut(s) 911
BsuRI GGCC 9 cut(s) 235, 378, 609, 718, 723, 841, 863, 948, 1164
BtgI CCRYGG 1 cut(s) 724
BtgZI GCGATG 2 cut(s) 669, 690
BtsCI GGATG 2 cut(s) 164, 1089
BtsIMutI CAGTG 2 cut(s) 225, 792
BveI ACCTGC 2 cut(s) 741, 1186
Cac8I GCNNGC 7 cut(s) 160, 279, 506, 686, 716, 843, 1044
CfoI GCGC 2 cut(s) 164, 638
Cfr10I RCCGGY 4 cut(s) 247, 609, 632, 650
Cfr13I GGNCC 6 cut(s) 215, 233, 234, 396, 716, 721
CseI GACGC 1 cut(s) 652
Csp6I GTAC 2 cut(s) 754, 1137
CspAI ACCGGT 1 cut(s) 247
CspCI CAANNNNNGTGG 2 cut(s) 840, 875
CviAII CATG 4 cut(s) 159, 554, 758, 837
CviQI GTAC 2 cut(s) 754, 1137
DdeI CTNAG 2 cut(s) 355, 865
DpnI GATC 5 cut(s) 385, 560, 644, 983, 1026
DpnII GATC 5 cut(s) 383, 558, 642, 981, 1024
EaeI YGGCCR 5 cut(s) 376, 607, 839, 946, 1162
Eco130I CCWWGG 3 cut(s) 102, 379, 745
Eco24I GRGCYC 1 cut(s) 237
Eco32I GATATC 2 cut(s) 48, 85
Eco47I GGWCC 2 cut(s) 215, 396
EcoNI CCTNNNNNAGG 1 cut(s) 774
EcoO109I RGGNCCY 2 cut(s) 233, 234
EcoRII CCWGG 1 cut(s) 571
EcoRV GATATC 2 cut(s) 48, 85
EcoT14I CCWWGG 3 cut(s) 102, 379, 745
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 3 cut(s) 102, 379, 745
Esp3I CGTCTC 1 cut(s) 670
FaeI CATG 4 cut(s) 162, 557, 761, 840
FatI CATG 4 cut(s) 158, 553, 757, 836
FauI CCCGC 1 cut(s) 707
FbaI TGATCA 1 cut(s) 558
Fnu4HI GCNGC 3 cut(s) 72, 1122, 1197
FokI GGATG 2 cut(s) 151, 1076
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 3 cut(s) 72, 1122, 1197
FspAI RTGCGCAY 1 cut(s) 163
FspBI CTAG 1 cut(s) 746
FspI TGCGCA 1 cut(s) 163
GlaI GCGC 2 cut(s) 163, 637
GluI GCNGC 3 cut(s) 72, 1122, 1197
HaeIII GGCC 9 cut(s) 235, 378, 609, 718, 723, 841, 863, 948, 1164
HapII CCGG 7 cut(s) 130, 248, 284, 610, 633, 651, 719
HgaI GACGC 1 cut(s) 652
HhaI GCGC 2 cut(s) 164, 638
Hin1II CATG 4 cut(s) 162, 557, 761, 840
Hin6I GCGC 2 cut(s) 162, 636
HinP1I GCGC 2 cut(s) 162, 636
HincII GTYRAC 1 cut(s) 348
HindII GTYRAC 1 cut(s) 348
HindIII AAGCTT 1 cut(s) 1040
HinfI GANTC 3 cut(s) 453, 597, 780
HpaI GTTAAC 1 cut(s) 348
HpaII CCGG 7 cut(s) 130, 248, 284, 610, 633, 651, 719
HphI GGTGA 4 cut(s) 556, 587, 622, 718
Hpy166II GTNNAC 1 cut(s) 348
Hpy188I TCNGA 3 cut(s) 177, 332, 868
Hpy188III TCNNGA 5 cut(s) 43, 357, 526, 832, 1000
Hpy8I GTNNAC 1 cut(s) 348
Hpy99I CGWCG 1 cut(s) 973
HpyAV CCTTC 1 cut(s) 260
HpyCH4III ACNGT 6 cut(s) 184, 352, 728, 787, 857, 938
HpyCH4IV ACGT 3 cut(s) 522, 540, 1143
HpyF10VI GCNNNNNNNGC 9 cut(s) 71, 298, 660, 681, 720, 741, 993, 1065, 1193
HpyF3I CTNAG 2 cut(s) 355, 865
HpySE526I ACGT 3 cut(s) 522, 540, 1143
Hsp92II CATG 4 cut(s) 162, 557, 761, 840
HspAI GCGC 2 cut(s) 162, 636
KpnI GGTACC 1 cut(s) 757
Ksp22I TGATCA 1 cut(s) 558
KspAI GTTAAC 1 cut(s) 348
Kzo9I GATC 5 cut(s) 383, 558, 642, 981, 1024
LmnI GCTCC 3 cut(s) 98, 286, 741
Lsp1109I GCAGC 2 cut(s) 58, 1108
LweI GCATC 1 cut(s) 173
MaeI CTAG 1 cut(s) 746
MaeII ACGT 3 cut(s) 522, 540, 1143
MaeIII GTNAC 5 cut(s) 306, 541, 772, 781, 1085
MalI GATC 5 cut(s) 385, 560, 644, 983, 1026
MboI GATC 5 cut(s) 383, 558, 642, 981, 1024
MboII GAAGA 1 cut(s) 916
MhlI GDGCHC 3 cut(s) 182, 237, 1016
MlsI TGGCCA 4 cut(s) 378, 841, 948, 1164
MluCI AATT 5 cut(s) 11, 469, 888, 1080, 1167
MluNI TGGCCA 4 cut(s) 378, 841, 948, 1164
MlyI GAGTC 1 cut(s) 789
MnlI CCTC 5 cut(s) 353, 819, 874, 874, 919
Mox20I TGGCCA 4 cut(s) 378, 841, 948, 1164
MscI TGGCCA 4 cut(s) 378, 841, 948, 1164
MseI TTAA 4 cut(s) 138, 347, 978, 1201
MslI CAYNNNNRTG 3 cut(s) 185, 890, 1132
Msp20I TGGCCA 4 cut(s) 378, 841, 948, 1164
MspI CCGG 7 cut(s) 130, 248, 284, 610, 633, 651, 719
MspR9I CCNGG 3 cut(s) 131, 573, 720
Mva1269I GAATGC 2 cut(s) 255, 1059
MvaI CCWGG 1 cut(s) 573
MwoI GCNNNNNNNGC 9 cut(s) 71, 298, 660, 681, 720, 741, 993, 1065, 1193
NciI CCSGG 2 cut(s) 131, 720
NdeII GATC 5 cut(s) 383, 558, 642, 981, 1024
NlaIII CATG 4 cut(s) 162, 557, 761, 840
NlaIV GGNNCC 6 cut(s) 100, 235, 271, 282, 743, 755
NmuCI GTSAC 5 cut(s) 306, 541, 772, 781, 1085
NsbI TGCGCA 1 cut(s) 163
NspI RCATGY 2 cut(s) 162, 557
OliI CACNNNNGTG 1 cut(s) 185
PaeI GCATGC 1 cut(s) 162
PaqCI CACCTGC 1 cut(s) 1186
PciI ACATGT 1 cut(s) 553
PctI GAATGC 2 cut(s) 255, 1059
PfeI GAWTC 2 cut(s) 453, 597
PinAI ACCGGT 1 cut(s) 247
PkrI GCNGC 3 cut(s) 73, 1123, 1198
PleI GAGTC 1 cut(s) 788
PpsI GAGTC 1 cut(s) 788
PscI ACATGT 1 cut(s) 553
PshBI ATTAAT 1 cut(s) 138
Psp6I CCWGG 1 cut(s) 571
PspGI CCWGG 1 cut(s) 571
PspN4I GGNNCC 6 cut(s) 100, 235, 271, 282, 743, 755
PspOMI GGGCCC 1 cut(s) 233
PspPI GGNCC 6 cut(s) 215, 233, 234, 396, 716, 721
PstI CTGCAG 3 cut(s) 76, 279, 1198
RsaI GTAC 2 cut(s) 755, 1138
RsaNI GTAC 2 cut(s) 754, 1137
RseI CAYNNNNRTG 3 cut(s) 185, 890, 1132
SaqAI TTAA 4 cut(s) 138, 347, 978, 1201
SatI GCNGC 3 cut(s) 72, 1122, 1197
Sau3AI GATC 5 cut(s) 383, 558, 642, 981, 1024
Sau96I GGNCC 6 cut(s) 215, 233, 234, 396, 716, 721
SbfI CCTGCAGG 1 cut(s) 279
SchI GAGTC 1 cut(s) 789
ScrFI CCNGG 3 cut(s) 131, 573, 720
SdaI CCTGCAGG 1 cut(s) 279
SduI GDGCHC 3 cut(s) 182, 237, 1016
SfaNI GCATC 1 cut(s) 173
SfcI CTRYAG 3 cut(s) 72, 275, 1194
SgrAI CRCCGGYG 1 cut(s) 632
SinI GGWCC 2 cut(s) 215, 396
SmiMI CAYNNNNRTG 3 cut(s) 185, 890, 1132
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
SphI GCATGC 1 cut(s) 162
Sse8387I CCTGCAGG 1 cut(s) 279
Sse9I AATT 5 cut(s) 11, 469, 888, 1080, 1167
SsiI CCGC 2 cut(s) 567, 714
SspI AATATT 1 cut(s) 337
SspMI CTAG 1 cut(s) 746
StyD4I CCNGG 3 cut(s) 129, 571, 718
StyI CCWWGG 3 cut(s) 102, 379, 745
TaaI ACNGT 6 cut(s) 184, 352, 728, 787, 857, 938
TaiI ACGT 3 cut(s) 525, 543, 1146
TaqI TCGA 2 cut(s) 617, 971
TasI AATT 5 cut(s) 11, 469, 888, 1080, 1167
TatI WGTACW 1 cut(s) 1136
TfiI GAWTC 2 cut(s) 453, 597
Tru1I TTAA 4 cut(s) 138, 347, 978, 1201
Tru9I TTAA 4 cut(s) 138, 347, 978, 1201
TscAI CASTG 2 cut(s) 225, 792
TseFI GTSAC 5 cut(s) 306, 541, 772, 781, 1085
TseI GCWGC 3 cut(s) 71, 1121, 1196
Tsp45I GTSAC 5 cut(s) 306, 541, 772, 781, 1085
TspDTI ATGAA 1 cut(s) 777
TspRI CASTG 2 cut(s) 225, 792
VpaK11BI GGWCC 2 cut(s) 215, 396
VspI ATTAAT 1 cut(s) 138
XagI CCTNNNNNAGG 1 cut(s) 774
XapI RAATTY 2 cut(s) 11, 1167
XceI RCATGY 2 cut(s) 162, 557
XmaJI CCTAGG 1 cut(s) 745
XspI CTAG 1 cut(s) 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.