MD17G1112500.v1.1

Nuclear protein Es2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010388
Physical Location & Seq
Reverse (-)
9640511 .. 9642016
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1112500.v1.1.491

Sequence Viewer

Length: 1506 bp
ATGCTTCTCACTCCGGGTCACTCTCCTCGCCACCTCTCATCCCCTTCGCCCTCTGCAAATTCCGATGCTTCCGTCCAAAACCCTACGAATTCCACCCAAATCACTCCCAAGAACCCTAAAGGCCATCCCAAGGTCCTCGACGAGGACACCTATGTCGCCGCGATCGAGAAGATCATCGAAAGGGACTTCTTTCCCGATATTTCCAAGCTCCGGGACAGGCTCGACTGGCTTGAAGCGATCAAGACGCGCGACCCGGTTCAGATCCGAGATGCCCAGTTGAAGATCATCGAGCGCCGCGGAAAGAAGGTAACTCATAATCCTGACCCTGATGGTAGAACCCCAGGTTCGACTTTTATGCGTAGTTATACCCCTGCCGATGAATTTGATGGTAAAACCCCGGTTGCTTCGAATGTGGGATTGTCTGGTGAGGTAGGGTCTAGCGGCGGTGATGATAGCGTGGAGGGATCATTGTCGCTTGATCAGTTTTTAAGGCGGTATACTAGTGAGGACAATTATAGTTTTTCGAATATTTTGGAGAAGGTGAATAGGAAGAGGAAGGAGAGATATGAGTATTTGACGGAAGGCGAAAAGGAGGACGTTAAATCAATTGAGGATGTGAAGAGGGATAGGATTACAGATGGGTATGGGACGTCAGATCAGCCGCCTAGTACATTGGATGGGTGGAAGTATACGGCGAAGAATTTGCTGATGTACCATCCAGCTGATCGGGGTGAGGCTCCCTTGACAGAGGAGGAACGTGCGAATAGGATGAAGAGTTTGGAGAAGGAAATTAATCGACCCAACACGCGATTTAATGGGAAGACGATGGATTCCAGGCCAAAGGAGGATGCTATGGCTGAGTTTCTTTATACCACAGTTGCAGGTGCAACCCCAGTTATTATGGATAGAGAAGGGGACAAGTTGAAGAAGTATGATTTGGATGATTTGAGAAAGACTCCCAATCTGTTTTATGTGGAATCGGAGAAGAAAGCCGATAATGGTTATCGTTTTGTGAAGACACCCTCGCCTGCACCGGGTGTTGATGAATCACCGTTCATTACATGGGGTGAAATTGAAGGGACACCTCTGAGGTTGGATCTTGAGGACAATCCAGTGGATATTGGTGGCGGTGAAGGGCCTCAATATAGGATCCCATGCCCGCCTGTAAGAGACGAAAAGGCTCACTCACTGTCAAGGGAAGCTGCACGCAAATTGAGGGAAAGGTCAAAATTGTTTCACAAGCCACCTCTGCCATCGCCTTCTAGAGGTGGCAGTGCCAGTCCAAGTGTGAGGACACTCTCCCCTGCTGCTCAGAAGTTTGTAAGAAATGCAATGGGCAAGTCCTCATCTTCTATTGATGAAACTCTTCGTGCCAGTTACCGAGGTTCAAGTCCAGGGTTTACTACTCCTAAAGGTGGAAGGAGTGTGTCGAGGCTTGGAAGCCATGCTAGTATCGTATCAAGATCACCTTCTGCTGCAAGGGAGGGCACTAATCCTCATTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Es2

Protein Analysis

502

Amino Acids

55.68

Weight (kDa)

8.56

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Es2 PF09751 43 - 440 2.3e-105 Nuclear protein Es2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 872
AasI GACNNNNNNGTC 1 cut(s) 152
AatII GACGTC 1 cut(s) 653
Acc36I ACCTGC 1 cut(s) 872
AccI GTMKAC 2 cut(s) 497, 689
AccII CGCG 5 cut(s) 161, 247, 249, 297, 808
AciI CCGC 9 cut(s) 159, 295, 297, 441, 444, 493, 662, 1128, 1160
AclWI GGATC 5 cut(s) 256, 472, 1104, 1144, 1157
AcsI RAATTY 4 cut(s) 58, 88, 380, 700
AcyI GRCGYC 1 cut(s) 650
AdeI CACNNNGTG 1 cut(s) 1037
AfaI GTAC 2 cut(s) 670, 713
AfiI CCNNNNNNNGG 6 cut(s) 130, 142, 210, 1034, 1265, 1415
AgsI TTSAA 5 cut(s) 233, 280, 925, 1076, 1389
AhlI ACTAGT 1 cut(s) 500
AjnI CCWGG 3 cut(s) 340, 833, 1393
AjuI GAANNNNNNNTTGG 2 cut(s) 920, 952
AluBI AGCT 3 cut(s) 208, 722, 1202
AluI AGCT 3 cut(s) 208, 722, 1202
Alw26I GTCTC 1 cut(s) 1164
AlwI GGATC 5 cut(s) 256, 472, 1104, 1144, 1157
AoxI GGCC 3 cut(s) 121, 836, 1136
ApeKI GCWGC 3 cut(s) 1202, 1307, 1475
ApoI RAATTY 4 cut(s) 58, 88, 380, 700
AseI ATTAAT 1 cut(s) 792
Asp700I GAANNNNTTC 1 cut(s) 1365
AspLEI GCGC 2 cut(s) 249, 294
AspS9I GGNCC 2 cut(s) 133, 1136
AsuC2I CCSGG 5 cut(s) 15, 212, 254, 398, 1035
AsuHPI GGTGA 8 cut(s) 437, 458, 553, 743, 1041, 1079, 1142, 1458
AsuII TTCGAA 2 cut(s) 407, 524
AvaII GGWCC 1 cut(s) 133
BaeGI GKGCMC 1 cut(s) 1490
BamHI GGATCC 1 cut(s) 1149
BbsI GAAGAC 2 cut(s) 827, 1022
BbvI GCAGC 3 cut(s) 1189, 1294, 1462
BccI CCATC 8 cut(s) 132, 323, 380, 632, 671, 723, 820, 1261
BceAI ACGGC 1 cut(s) 708
BcgI CGANNNNNNTGC 4 cut(s) 337, 371, 685, 719
BciT130I CCWGG 3 cut(s) 342, 835, 1395
BclI TGATCA 1 cut(s) 478
BcnI CCSGG 5 cut(s) 15, 212, 254, 398, 1035
BcoDI GTCTC 1 cut(s) 1164
BcuI ACTAGT 1 cut(s) 500
BfaI CTAG 5 cut(s) 438, 501, 666, 1263, 1449
BfoI RGCGCY 1 cut(s) 295
BfuAI ACCTGC 1 cut(s) 872
BisI GCNGC 7 cut(s) 159, 295, 442, 662, 1203, 1308, 1476
BlsI GCNGC 7 cut(s) 160, 296, 443, 663, 1204, 1309, 1477
Bme1390I CCNGG 8 cut(s) 15, 212, 254, 342, 398, 835, 1035, 1395
Bme18I GGWCC 1 cut(s) 133
BmgT120I GGNCC 2 cut(s) 133, 1136
BmiI GGNNCC 2 cut(s) 738, 1151
BmrFI CCNGG 8 cut(s) 15, 212, 254, 342, 398, 835, 1035, 1395
BmrI ACTGGG 2 cut(s) 268, 887
BmsI GCATC 3 cut(s) 55, 259, 838
BmuI ACTGGG 2 cut(s) 268, 887
BpiI GAAGAC 2 cut(s) 827, 1022
BplI GAGNNNNNCTC 2 cut(s) 940, 972
Bpu14I TTCGAA 2 cut(s) 407, 524
BpuEI CTTGAG 1 cut(s) 1121
BpuMI CCSGG 5 cut(s) 15, 212, 254, 398, 1035
BsaHI GRCGYC 1 cut(s) 650
BsaJI CCNNGG 6 cut(s) 129, 295, 340, 396, 1381, 1394
Bsc4I CCNNNNNNNGG 6 cut(s) 130, 142, 210, 1034, 1265, 1415
Bse1I ACTGG 6 cut(s) 230, 274, 893, 1112, 1278, 1374
Bse3DI GCAATG 1 cut(s) 1338
BseBI CCWGG 3 cut(s) 342, 835, 1395
BseDI CCNNGG 6 cut(s) 129, 295, 340, 396, 1381, 1394
BseGI GGATG 8 cut(s) 38, 124, 619, 682, 715, 774, 853, 946
BseLI CCNNNNNNNGG 6 cut(s) 130, 142, 210, 1034, 1265, 1415
BseMI GCAATG 1 cut(s) 1338
BseMII CTCAG 3 cut(s) 849, 1079, 1325
BseNI ACTGG 6 cut(s) 230, 274, 893, 1112, 1278, 1374
BseRI GAGGAG 2 cut(s) 15, 764
BseSI GKGCMC 1 cut(s) 1490
BseXI GCAGC 3 cut(s) 1189, 1294, 1462
BsgI GTGCAG 2 cut(s) 1014, 1188
Bsh1236I CGCG 5 cut(s) 161, 247, 249, 297, 808
Bsh1285I CGRYCG 1 cut(s) 165
BshFI GGCC 3 cut(s) 123, 838, 1138
BsiEI CGRYCG 1 cut(s) 165
BsiSI CCGG 5 cut(s) 14, 211, 254, 398, 1034
BslFI GGGAC 5 cut(s) 197, 227, 661, 929, 1093
BslI CCNNNNNNNGG 6 cut(s) 130, 142, 210, 1034, 1265, 1415
BsmAI GTCTC 1 cut(s) 1164
BsmBI CGTCTC 1 cut(s) 1164
BsmFI GGGAC 5 cut(s) 197, 227, 661, 929, 1093
BsnI GGCC 3 cut(s) 123, 838, 1138
Bsp119I TTCGAA 2 cut(s) 407, 524
Bsp1286I GDGCHC 1 cut(s) 1490
BspACI CCGC 9 cut(s) 159, 295, 297, 441, 444, 493, 662, 1128, 1160
BspANI GGCC 3 cut(s) 123, 838, 1138
BspCNI CTCAG 3 cut(s) 850, 1080, 1324
BspFNI CGCG 5 cut(s) 161, 247, 249, 297, 808
BspLI GGNNCC 2 cut(s) 738, 1151
BspMI ACCTGC 1 cut(s) 872
BspPI GGATC 5 cut(s) 256, 472, 1104, 1144, 1157
BspT104I TTCGAA 2 cut(s) 407, 524
BsrDI GCAATG 1 cut(s) 1338
BsrI ACTGG 6 cut(s) 230, 274, 893, 1112, 1278, 1374
BssECI CCNNGG 6 cut(s) 129, 295, 340, 396, 1381, 1394
BssNAI GTATAC 2 cut(s) 498, 690
BssNI GRCGYC 1 cut(s) 650
BssT1I CCWWGG 1 cut(s) 129
Bst1107I GTATAC 2 cut(s) 498, 690
Bst2UI CCWGG 3 cut(s) 342, 835, 1395
Bst4CI ACNGT 3 cut(s) 877, 1053, 1191
Bst6I CTCTTC 4 cut(s) 545, 614, 767, 1371
BstACI GRCGYC 1 cut(s) 650
BstBI TTCGAA 2 cut(s) 407, 524
BstC8I GCNNGC 3 cut(s) 1029, 1160, 1207
BstDEI CTNAG 3 cut(s) 858, 1088, 1311
BstDSI CCRYGG 1 cut(s) 295
BstENI CCTNNNNNAGG 2 cut(s) 140, 1263
BstF5I GGATG 8 cut(s) 38, 124, 619, 682, 715, 774, 853, 946
BstFNI CGCG 5 cut(s) 161, 247, 249, 297, 808
BstH2I RGCGCY 1 cut(s) 295
BstHHI GCGC 2 cut(s) 249, 294
BstMAI GTCTC 1 cut(s) 1164
BstMCI CGRYCG 1 cut(s) 165
BstMWI GCNNNNNNNGC 2 cut(s) 226, 1249
BstNI CCWGG 3 cut(s) 342, 835, 1395
BstSCI CCNGG 8 cut(s) 13, 210, 252, 340, 396, 833, 1033, 1393
BstSLI GKGCMC 1 cut(s) 1490
BstUI CGCG 5 cut(s) 161, 247, 249, 297, 808
BstV1I GCAGC 3 cut(s) 1189, 1294, 1462
BstV2I GAAGAC 2 cut(s) 827, 1022
BstX2I RGATCY 3 cut(s) 261, 1096, 1149
BstYI RGATCY 3 cut(s) 261, 1096, 1149
BstZ17I GTATAC 2 cut(s) 498, 690
BsuRI GGCC 3 cut(s) 123, 838, 1138
BtgI CCRYGG 1 cut(s) 295
BtgZI GCGATG 1 cut(s) 1239
BtsCI GGATG 8 cut(s) 38, 124, 619, 682, 715, 774, 853, 946
BtsI GCAGTG 1 cut(s) 1279
BtsIMutI CAGTG 3 cut(s) 1119, 1187, 1279
BveI ACCTGC 1 cut(s) 872
Cac8I GCNNGC 3 cut(s) 1029, 1160, 1207
CfoI GCGC 2 cut(s) 249, 294
Cfr13I GGNCC 2 cut(s) 133, 1136
Cfr42I CCGCGG 1 cut(s) 298
CseI GACGC 1 cut(s) 253
Csp6I GTAC 2 cut(s) 669, 712
CviAII CATG 3 cut(s) 1062, 1155, 1445
CviQI GTAC 2 cut(s) 669, 712
DdeI CTNAG 3 cut(s) 858, 1088, 1311
DraIII CACNNNGTG 1 cut(s) 1037
DrdI GACNNNNNNGTC 1 cut(s) 152
DseDI GACNNNNNNGTC 1 cut(s) 152
Eam1104I CTCTTC 4 cut(s) 545, 614, 767, 1371
EarI CTCTTC 4 cut(s) 545, 614, 767, 1371
Eco130I CCWWGG 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 133
EcoNI CCTNNNNNAGG 2 cut(s) 140, 1263
EcoO109I RGGNCCY 2 cut(s) 133, 1136
EcoRI GAATTC 1 cut(s) 88
EcoRII CCWGG 3 cut(s) 340, 833, 1393
EcoT14I CCWWGG 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 129
Esp3I CGTCTC 1 cut(s) 1164
FaeI CATG 3 cut(s) 1065, 1158, 1448
FalI AAGNNNNNCTT 2 cut(s) 1453, 1485
FaqI GGGAC 5 cut(s) 197, 227, 661, 929, 1093
FatI CATG 3 cut(s) 1061, 1154, 1444
FauI CCCGC 1 cut(s) 1167
FbaI TGATCA 1 cut(s) 478
FblI GTMKAC 2 cut(s) 497, 689
Fnu4HI GCNGC 7 cut(s) 159, 295, 442, 662, 1203, 1308, 1476
FokI GGATG 8 cut(s) 25, 111, 626, 689, 702, 781, 860, 953
Fsp4HI GCNGC 7 cut(s) 159, 295, 442, 662, 1203, 1308, 1476
FspBI CTAG 5 cut(s) 438, 501, 666, 1263, 1449
GlaI GCGC 2 cut(s) 248, 293
GluI GCNGC 7 cut(s) 159, 295, 442, 662, 1203, 1308, 1476
HaeII RGCGCY 1 cut(s) 295
HaeIII GGCC 3 cut(s) 123, 838, 1138
HapII CCGG 5 cut(s) 14, 211, 254, 398, 1034
HgaI GACGC 1 cut(s) 253
HhaI GCGC 2 cut(s) 249, 294
Hin1I GRCGYC 1 cut(s) 650
Hin1II CATG 3 cut(s) 1065, 1158, 1448
Hin6I GCGC 2 cut(s) 247, 292
HinP1I GCGC 2 cut(s) 247, 292
HinfI GANTC 4 cut(s) 830, 955, 977, 1046
HpaII CCGG 5 cut(s) 14, 211, 254, 398, 1034
HphI GGTGA 8 cut(s) 437, 458, 553, 743, 1041, 1079, 1142, 1458
Hpy166II GTNNAC 3 cut(s) 498, 690, 1401
Hpy188I TCNGA 7 cut(s) 64, 261, 266, 655, 982, 1089, 1314
Hpy188III TCNNGA 7 cut(s) 166, 194, 241, 320, 1100, 1263, 1461
Hpy8I GTNNAC 3 cut(s) 498, 690, 1401
Hpy99I CGWCG 1 cut(s) 143
HpyCH4III ACNGT 3 cut(s) 877, 1053, 1191
HpyCH4IV ACGT 3 cut(s) 597, 650, 757
HpyCH4V TGCA 7 cut(s) 56, 881, 887, 1031, 1205, 1331, 1478
HpyF10VI GCNNNNNNNGC 2 cut(s) 226, 1249
HpyF3I CTNAG 3 cut(s) 858, 1088, 1311
HpySE526I ACGT 3 cut(s) 597, 650, 757
Hsp92I GRCGYC 1 cut(s) 650
Hsp92II CATG 3 cut(s) 1065, 1158, 1448
HspAI GCGC 2 cut(s) 247, 292
Ksp22I TGATCA 1 cut(s) 478
KspI CCGCGG 1 cut(s) 298
LmnI GCTCC 2 cut(s) 213, 742
Lsp1109I GCAGC 3 cut(s) 1189, 1294, 1462
LweI GCATC 3 cut(s) 55, 259, 838
MaeI CTAG 5 cut(s) 438, 501, 666, 1263, 1449
MaeII ACGT 3 cut(s) 597, 650, 757
MaeIII GTNAC 3 cut(s) 17, 307, 1376
MfeI CAATTG 1 cut(s) 606
MflI RGATCY 3 cut(s) 261, 1096, 1149
MhlI GDGCHC 1 cut(s) 1490
MlyI GAGTC 1 cut(s) 949
MmeI TCCRAC 1 cut(s) 1074
MroXI GAANNNNTTC 1 cut(s) 1365
MseI TTAA 4 cut(s) 488, 600, 792, 813
MspA1I CMGCKG 2 cut(s) 297, 722
MspI CCGG 5 cut(s) 14, 211, 254, 398, 1034
MspR9I CCNGG 8 cut(s) 15, 212, 254, 342, 398, 835, 1035, 1395
MunI CAATTG 1 cut(s) 606
MvaI CCWGG 3 cut(s) 342, 835, 1395
MvnI CGCG 5 cut(s) 161, 247, 249, 297, 808
MwoI GCNNNNNNNGC 2 cut(s) 226, 1249
NciI CCSGG 5 cut(s) 15, 212, 254, 398, 1035
NlaIII CATG 3 cut(s) 1065, 1158, 1448
NlaIV GGNNCC 2 cut(s) 738, 1151
NmuCI GTSAC 1 cut(s) 17
NspV TTCGAA 2 cut(s) 407, 524
PaqCI CACCTGC 1 cut(s) 872
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PdmI GAANNNNTTC 1 cut(s) 1365
PfeI GAWTC 3 cut(s) 830, 977, 1046
PfoI TCCNGGA 1 cut(s) 210
PkrI GCNGC 7 cut(s) 160, 296, 443, 663, 1204, 1309, 1477
Ple19I CGATCG 1 cut(s) 165
PleI GAGTC 1 cut(s) 949
PpsI GAGTC 1 cut(s) 949
PpuMI RGGWCCY 1 cut(s) 133
PshBI ATTAAT 1 cut(s) 792
Psp5II RGGWCCY 1 cut(s) 133
Psp6I CCWGG 3 cut(s) 340, 833, 1393
PspGI CCWGG 3 cut(s) 340, 833, 1393
PspN4I GGNNCC 2 cut(s) 738, 1151
PspPI GGNCC 2 cut(s) 133, 1136
PspPPI RGGWCCY 1 cut(s) 133
PsuI RGATCY 3 cut(s) 261, 1096, 1149
PvuI CGATCG 1 cut(s) 165
PvuII CAGCTG 1 cut(s) 722
RsaI GTAC 2 cut(s) 670, 713
RsaNI GTAC 2 cut(s) 669, 712
SacII CCGCGG 1 cut(s) 298
SaqAI TTAA 4 cut(s) 488, 600, 792, 813
SatI GCNGC 7 cut(s) 159, 295, 442, 662, 1203, 1308, 1476
Sau96I GGNCC 2 cut(s) 133, 1136
SchI GAGTC 1 cut(s) 949
ScrFI CCNGG 8 cut(s) 15, 212, 254, 342, 398, 835, 1035, 1395
SduI GDGCHC 1 cut(s) 1490
SfaNI GCATC 3 cut(s) 55, 259, 838
Sfr303I CCGCGG 1 cut(s) 298
SfuI TTCGAA 2 cut(s) 407, 524
SgrBI CCGCGG 1 cut(s) 298
SinI GGWCC 1 cut(s) 133
SmlI CTYRAG 1 cut(s) 1100
SmoI CTYRAG 1 cut(s) 1100
SpeI ACTAGT 1 cut(s) 500
SsiI CCGC 9 cut(s) 159, 295, 297, 441, 444, 493, 662, 1128, 1160
SspI AATATT 1 cut(s) 529
SspMI CTAG 5 cut(s) 438, 501, 666, 1263, 1449
StyD4I CCNGG 8 cut(s) 13, 210, 252, 340, 396, 833, 1033, 1393
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 3 cut(s) 877, 1053, 1191
TaiI ACGT 3 cut(s) 600, 653, 760
TatI WGTACW 1 cut(s) 668
TauI GCSGC 4 cut(s) 161, 297, 444, 664
TfiI GAWTC 3 cut(s) 830, 977, 1046
Tru1I TTAA 4 cut(s) 488, 600, 792, 813
Tru9I TTAA 4 cut(s) 488, 600, 792, 813
TscAI CASTG 3 cut(s) 1119, 1194, 1279
TseFI GTSAC 1 cut(s) 17
TseI GCWGC 3 cut(s) 1202, 1307, 1475
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 5 cut(s) 393, 785, 1045, 1059, 1374
TspGWI ACGGA 2 cut(s) 61, 593
TspRI CASTG 3 cut(s) 1119, 1194, 1279
VpaK11BI GGWCC 1 cut(s) 133
VspI ATTAAT 1 cut(s) 792
XagI CCTNNNNNAGG 2 cut(s) 140, 1263
XapI RAATTY 4 cut(s) 58, 88, 380, 700
XbaI TCTAGA 1 cut(s) 1262
XmiI GTMKAC 2 cut(s) 497, 689
XmnI GAANNNNTTC 1 cut(s) 1365
XspI CTAG 5 cut(s) 438, 501, 666, 1263, 1449
ZraI GACGTC 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.