pycom17g10420

Nuclear protein Es2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0010388
Physical Location & Seq
Reverse (-)
8019692 .. 8021949
2258 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g10420.1

Sequence Viewer

Length: 1407 bp
ATGCTTCTCACTCCGGGTCACTCTCCTCGCCACCTCTCATCCCCTTCGCCCTCTGCAAATTCTGATGCTTCCGTCCAAAACCCTACAAATTCCACCCAAATCACACCCAAGAACCCTAAAGGCCATCCCAAGGTCCTCGACGAGGACACCTATGTCGCCGCGATCGAGAAGATCATCGAAAGGGACTTCTTTCCCGATATTTCCAAGCTTCGGGACCGCCTCGACTGGCTCGAAGCGATCAAGACCCGCGACCCGATTCAGATCCGAGATGCCCAGTTGAAGATCATCGAGCGCCGCGGAAAGAAGGTAACTCATAATCCTGACCCTGATGGTAGAACCCCAGGTTCGACTTTTATGCGTAGTTATACCCCTGCCGATGAATTTGATGGTAAAACCCCGAAAACCCCGGGTGCTTCGAGTGTGGGATTGTCTGGTGAGGTAGGGTCTAGCGGCGGTGATGATAGCGTGGAGGGATCATTGTCGCTTGATCAGTTTTTAAGGCGGTATACGAGTGAGGACAATTATAGTTTTTCGAATATTTTGGAGAAGGTGAATAGGAAGAGGAAGGAGAGATATGAGTATTTGACGGAAGGCGAAAAGGAGGAGGTTAAATCGATTGAGGATGTGAAGAGGGATAGGATTACAGATGGGTATGGGACGTCGGATCAGCCGCCTAGTACATTGGATGGGTGGAAGTATACGGCAAAGAATTTGTTGATGTACCATCCAGCTGATCGGGGTGAGGCTCCCTTGACTGAGGAAGAACGTGCGAATAGGATGAAGAGTTTGGAGAAGGAAATTAATCGACCCAACACGCGATTTAATGGGAAGACGATGGATTCCAGGCCAAAGGAGGATGCTATGGCTGAGTTTCTCTATACCACAGTTGCAGGTGCAACCCCAGTTATTATGGATAGAGATGGGGATAAGTTGAAGAAGTATGATTTGGATGATTTGAGAAAGACGCCCAATCCGTTTTATGTGGAATCGGAGAAGAAAGCAGATAATGGTTATCGTTTTGTGAAGACACCCTCGCCTGCACCGGGTGTTGATGAATCACCGTTTATTACATGGGGTGAAATTGAAGGGACACCTCTGAGGTTGGATCTTGAGGACAATCCAGTTGATATTGGTGGCAGTGAAGGGCCTCAATATAGGATCCCATGCCCGCCTGTAAGAGACGAAAAGGCTCACTCGCTGTCAAGGGAAGCTGCACGCAAATTGAGGGAAAGGTCAAAATTGTTTCACAAGCCACCTCTGCCGTCGCCTTCTAGAGGTGGCAGTGCCAGTCCAAGTGCGATGAACTCAGTTCCTTGTTCTGGGGAGAAAAAAAGGGTTTCTGGTTTGAGAAATATTTGGAAATGTTGGATTCAGACACCGGTGCTGTGTAAACTTTATTCTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Es2

Protein Analysis

469

Amino Acids

52.49

Weight (kDa)

7.07

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Es2 PF09751 43 - 435 1.3e-102 Nuclear protein Es2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 881
AasI GACNNNNNNGTC 1 cut(s) 152
AatII GACGTC 1 cut(s) 662
Acc36I ACCTGC 1 cut(s) 881
AccI GTMKAC 2 cut(s) 506, 698
AccII CGCG 4 cut(s) 161, 249, 297, 817
AclWI GGATC 6 cut(s) 256, 481, 672, 1113, 1153, 1166
AcsI RAATTY 4 cut(s) 58, 88, 380, 709
AcyI GRCGYC 2 cut(s) 659, 965
AdeI CACNNNGTG 1 cut(s) 1046
AfaI GTAC 2 cut(s) 679, 722
AfiI CCNNNNNNNGG 6 cut(s) 130, 142, 210, 1043, 1274, 1319
AgeI ACCGGT 1 cut(s) 1378
AgsI TTSAA 3 cut(s) 280, 934, 1085
AjnI CCWGG 2 cut(s) 340, 842
AjuI GAANNNNNNNTTGG 2 cut(s) 929, 961
AluBI AGCT 3 cut(s) 208, 731, 1211
AluI AGCT 3 cut(s) 208, 731, 1211
Alw26I GTCTC 1 cut(s) 1173
AlwI GGATC 6 cut(s) 256, 481, 672, 1113, 1153, 1166
Ama87I CYCGRG 1 cut(s) 406
AoxI GGCC 3 cut(s) 121, 845, 1145
ApeKI GCWGC 1 cut(s) 1211
ApoI RAATTY 4 cut(s) 58, 88, 380, 709
AseI ATTAAT 1 cut(s) 801
AsiGI ACCGGT 1 cut(s) 1378
AspLEI GCGC 1 cut(s) 294
AspS9I GGNCC 3 cut(s) 133, 214, 1145
AsuC2I CCSGG 4 cut(s) 15, 407, 408, 1044
AsuHPI GGTGA 6 cut(s) 446, 467, 562, 752, 1050, 1088
AsuII TTCGAA 1 cut(s) 533
AvaI CYCGRG 1 cut(s) 406
AvaII GGWCC 2 cut(s) 133, 214
BamHI GGATCC 1 cut(s) 1158
BbsI GAAGAC 2 cut(s) 836, 1031
BbvI GCAGC 1 cut(s) 1198
BccI CCATC 8 cut(s) 132, 323, 380, 641, 680, 732, 829, 914
BceAI ACGGC 2 cut(s) 717, 1246
BcgI CGANNNNNNTGC 2 cut(s) 337, 371
BciT130I CCWGG 2 cut(s) 342, 844
BclI TGATCA 1 cut(s) 487
BcnI CCSGG 4 cut(s) 15, 407, 408, 1044
BcoDI GTCTC 1 cut(s) 1173
BfaI CTAG 3 cut(s) 447, 675, 1272
BfoI RGCGCY 1 cut(s) 295
BfuAI ACCTGC 1 cut(s) 881
BisI GCNGC 5 cut(s) 159, 295, 451, 671, 1212
BlsI GCNGC 5 cut(s) 160, 296, 452, 672, 1213
Bme1390I CCNGG 6 cut(s) 15, 342, 407, 408, 844, 1044
Bme18I GGWCC 2 cut(s) 133, 214
BmeT110I CYCGRG 1 cut(s) 406
BmgT120I GGNCC 3 cut(s) 133, 214, 1145
BmiI GGNNCC 3 cut(s) 215, 747, 1160
BmrFI CCNGG 6 cut(s) 15, 342, 407, 408, 844, 1044
BmrI ACTGGG 2 cut(s) 268, 896
BmsI GCATC 3 cut(s) 55, 259, 847
BmuI ACTGGG 2 cut(s) 268, 896
BpiI GAAGAC 2 cut(s) 836, 1031
Bpu14I TTCGAA 1 cut(s) 533
BpuEI CTTGAG 1 cut(s) 1130
BpuMI CCSGG 4 cut(s) 15, 407, 408, 1044
Bsa29I ATCGAT 1 cut(s) 614
BsaBI GATNNNNATC 1 cut(s) 260
BsaHI GRCGYC 2 cut(s) 659, 965
BsaJI CCNNGG 5 cut(s) 129, 295, 340, 405, 406
BsaWI WCCGGW 1 cut(s) 1378
Bsc4I CCNNNNNNNGG 6 cut(s) 130, 142, 210, 1043, 1274, 1319
Bse118I RCCGGY 1 cut(s) 1378
Bse1I ACTGG 5 cut(s) 230, 274, 902, 1121, 1287
Bse8I GATNNNNATC 1 cut(s) 260
BseBI CCWGG 2 cut(s) 342, 844
BseCI ATCGAT 1 cut(s) 614
BseDI CCNNGG 5 cut(s) 129, 295, 340, 405, 406
BseGI GGATG 8 cut(s) 38, 124, 628, 691, 724, 783, 862, 955
BseJI GATNNNNATC 1 cut(s) 260
BseLI CCNNNNNNNGG 6 cut(s) 130, 142, 210, 1043, 1274, 1319
BseMII CTCAG 4 cut(s) 747, 858, 1088, 1320
BseNI ACTGG 5 cut(s) 230, 274, 902, 1121, 1287
BseRI GAGGAG 2 cut(s) 15, 617
BseXI GCAGC 1 cut(s) 1198
BsgI GTGCAG 2 cut(s) 1023, 1197
Bsh1236I CGCG 4 cut(s) 161, 249, 297, 817
Bsh1285I CGRYCG 1 cut(s) 165
BshFI GGCC 3 cut(s) 123, 847, 1147
BshTI ACCGGT 1 cut(s) 1378
BshVI ATCGAT 1 cut(s) 614
BsiEI CGRYCG 1 cut(s) 165
BsiHKCI CYCGRG 1 cut(s) 406
BsiSI CCGG 4 cut(s) 14, 407, 1043, 1379
BslFI GGGAC 4 cut(s) 197, 227, 670, 1102
BslI CCNNNNNNNGG 6 cut(s) 130, 142, 210, 1043, 1274, 1319
BsmAI GTCTC 1 cut(s) 1173
BsmBI CGTCTC 1 cut(s) 1173
BsmFI GGGAC 4 cut(s) 197, 227, 670, 1102
BsnI GGCC 3 cut(s) 123, 847, 1147
BsoBI CYCGRG 1 cut(s) 406
Bsp119I TTCGAA 1 cut(s) 533
BspANI GGCC 3 cut(s) 123, 847, 1147
BspCNI CTCAG 4 cut(s) 748, 859, 1089, 1319
BspDI ATCGAT 1 cut(s) 614
BspFNI CGCG 4 cut(s) 161, 249, 297, 817
BspLI GGNNCC 3 cut(s) 215, 747, 1160
BspMI ACCTGC 1 cut(s) 881
BspPI GGATC 6 cut(s) 256, 481, 672, 1113, 1153, 1166
BspT104I TTCGAA 1 cut(s) 533
BsrFI RCCGGY 1 cut(s) 1378
BsrI ACTGG 5 cut(s) 230, 274, 902, 1121, 1287
BssAI RCCGGY 1 cut(s) 1378
BssECI CCNNGG 5 cut(s) 129, 295, 340, 405, 406
BssNAI GTATAC 2 cut(s) 507, 699
BssNI GRCGYC 2 cut(s) 659, 965
BssT1I CCWWGG 1 cut(s) 129
Bst1107I GTATAC 2 cut(s) 507, 699
Bst2UI CCWGG 2 cut(s) 342, 844
Bst4CI ACNGT 2 cut(s) 886, 1062
Bst6I CTCTTC 3 cut(s) 554, 623, 776
BstACI GRCGYC 2 cut(s) 659, 965
BstBI TTCGAA 1 cut(s) 533
BstC8I GCNNGC 3 cut(s) 1038, 1169, 1216
BstDEI CTNAG 4 cut(s) 756, 867, 1097, 1306
BstDSI CCRYGG 1 cut(s) 295
BstENI CCTNNNNNAGG 2 cut(s) 140, 1272
BstF5I GGATG 8 cut(s) 38, 124, 628, 691, 724, 783, 862, 955
BstFNI CGCG 4 cut(s) 161, 249, 297, 817
BstH2I RGCGCY 1 cut(s) 295
BstHHI GCGC 1 cut(s) 294
BstMAI GTCTC 1 cut(s) 1173
BstMCI CGRYCG 1 cut(s) 165
BstMWI GCNNNNNNNGC 1 cut(s) 1258
BstNI CCWGG 2 cut(s) 342, 844
BstSCI CCNGG 6 cut(s) 13, 340, 405, 406, 842, 1042
BstUI CGCG 4 cut(s) 161, 249, 297, 817
BstV1I GCAGC 1 cut(s) 1198
BstV2I GAAGAC 2 cut(s) 836, 1031
BstX2I RGATCY 3 cut(s) 261, 1105, 1158
BstYI RGATCY 3 cut(s) 261, 1105, 1158
BstZ17I GTATAC 2 cut(s) 507, 699
Bsu15I ATCGAT 1 cut(s) 614
BsuRI GGCC 3 cut(s) 123, 847, 1147
BsuTUI ATCGAT 1 cut(s) 614
BtgI CCRYGG 1 cut(s) 295
BtgZI GCGATG 1 cut(s) 1313
BtsCI GGATG 8 cut(s) 38, 124, 628, 691, 724, 783, 862, 955
BtsI GCAGTG 2 cut(s) 1144, 1288
BtsIMutI CAGTG 2 cut(s) 1144, 1288
BveI ACCTGC 1 cut(s) 881
Cac8I GCNNGC 3 cut(s) 1038, 1169, 1216
CfoI GCGC 1 cut(s) 294
Cfr10I RCCGGY 1 cut(s) 1378
Cfr13I GGNCC 3 cut(s) 133, 214, 1145
Cfr42I CCGCGG 1 cut(s) 298
Cfr9I CCCGGG 1 cut(s) 406
ClaI ATCGAT 1 cut(s) 614
CseI GACGC 1 cut(s) 973
Csp6I GTAC 2 cut(s) 678, 721
CspAI ACCGGT 1 cut(s) 1378
CviAII CATG 2 cut(s) 1071, 1164
CviQI GTAC 2 cut(s) 678, 721
DdeI CTNAG 4 cut(s) 756, 867, 1097, 1306
DraIII CACNNNGTG 1 cut(s) 1046
DrdI GACNNNNNNGTC 1 cut(s) 152
DseDI GACNNNNNNGTC 1 cut(s) 152
Eam1104I CTCTTC 3 cut(s) 554, 623, 776
EarI CTCTTC 3 cut(s) 554, 623, 776
Eco130I CCWWGG 1 cut(s) 129
Eco47I GGWCC 2 cut(s) 133, 214
Eco88I CYCGRG 1 cut(s) 406
EcoNI CCTNNNNNAGG 2 cut(s) 140, 1272
EcoO109I RGGNCCY 2 cut(s) 133, 1145
EcoRII CCWGG 2 cut(s) 340, 842
EcoT14I CCWWGG 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 129
Esp3I CGTCTC 1 cut(s) 1173
FaeI CATG 2 cut(s) 1074, 1167
FaqI GGGAC 4 cut(s) 197, 227, 670, 1102
FatI CATG 2 cut(s) 1070, 1163
FauI CCCGC 2 cut(s) 254, 1176
FbaI TGATCA 1 cut(s) 487
FblI GTMKAC 2 cut(s) 506, 698
Fnu4HI GCNGC 5 cut(s) 159, 295, 451, 671, 1212
FokI GGATG 8 cut(s) 25, 111, 635, 698, 711, 790, 869, 962
Fsp4HI GCNGC 5 cut(s) 159, 295, 451, 671, 1212
FspBI CTAG 3 cut(s) 447, 675, 1272
GlaI GCGC 1 cut(s) 293
GluI GCNGC 5 cut(s) 159, 295, 451, 671, 1212
HaeII RGCGCY 1 cut(s) 295
HaeIII GGCC 3 cut(s) 123, 847, 1147
HapII CCGG 4 cut(s) 14, 407, 1043, 1379
HgaI GACGC 1 cut(s) 973
HhaI GCGC 1 cut(s) 294
Hin1I GRCGYC 2 cut(s) 659, 965
Hin1II CATG 2 cut(s) 1074, 1167
Hin6I GCGC 1 cut(s) 292
HinP1I GCGC 1 cut(s) 292
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 5 cut(s) 256, 839, 986, 1055, 1369
HpaII CCGG 4 cut(s) 14, 407, 1043, 1379
HphI GGTGA 6 cut(s) 446, 467, 562, 752, 1050, 1088
Hpy166II GTNNAC 3 cut(s) 507, 699, 1391
Hpy188I TCNGA 8 cut(s) 64, 261, 266, 664, 991, 1098, 1374, 1402
Hpy188III TCNNGA 7 cut(s) 166, 194, 212, 241, 320, 1109, 1272
Hpy8I GTNNAC 3 cut(s) 507, 699, 1391
Hpy99I CGWCG 3 cut(s) 143, 664, 1267
HpyAV CCTTC 9 cut(s) 54, 298, 541, 559, 584, 787, 1079, 1136, 1278
HpyCH4III ACNGT 2 cut(s) 886, 1062
HpyCH4IV ACGT 2 cut(s) 659, 766
HpyCH4V TGCA 5 cut(s) 56, 890, 896, 1040, 1214
HpyF10VI GCNNNNNNNGC 1 cut(s) 1258
HpyF3I CTNAG 4 cut(s) 756, 867, 1097, 1306
HpySE526I ACGT 2 cut(s) 659, 766
Hsp92I GRCGYC 2 cut(s) 659, 965
Hsp92II CATG 2 cut(s) 1074, 1167
HspAI GCGC 1 cut(s) 292
Ksp22I TGATCA 1 cut(s) 487
KspI CCGCGG 1 cut(s) 298
LmnI GCTCC 1 cut(s) 751
Lsp1109I GCAGC 1 cut(s) 1198
LweI GCATC 3 cut(s) 55, 259, 847
MaeI CTAG 3 cut(s) 447, 675, 1272
MaeII ACGT 2 cut(s) 659, 766
MaeIII GTNAC 2 cut(s) 17, 307
MflI RGATCY 3 cut(s) 261, 1105, 1158
MluCI AATT 9 cut(s) 58, 88, 380, 520, 709, 798, 1080, 1220, 1238
MmeI TCCRAC 3 cut(s) 642, 1083, 1346
MseI TTAA 4 cut(s) 497, 609, 801, 822
MspA1I CMGCKG 2 cut(s) 297, 731
MspI CCGG 4 cut(s) 14, 407, 1043, 1379
MspR9I CCNGG 6 cut(s) 15, 342, 407, 408, 844, 1044
MvaI CCWGG 2 cut(s) 342, 844
MvnI CGCG 4 cut(s) 161, 249, 297, 817
MwoI GCNNNNNNNGC 1 cut(s) 1258
NciI CCSGG 4 cut(s) 15, 407, 408, 1044
NlaIII CATG 2 cut(s) 1074, 1167
NlaIV GGNNCC 3 cut(s) 215, 747, 1160
NmuCI GTSAC 1 cut(s) 17
NspV TTCGAA 1 cut(s) 533
PaqCI CACCTGC 1 cut(s) 881
PcsI WCGNNNNNNNCGW 3 cut(s) 162, 228, 971
PfeI GAWTC 5 cut(s) 256, 839, 986, 1055, 1369
PinAI ACCGGT 1 cut(s) 1378
PkrI GCNGC 5 cut(s) 160, 296, 452, 672, 1213
Ple19I CGATCG 1 cut(s) 165
PpuMI RGGWCCY 1 cut(s) 133
PshBI ATTAAT 1 cut(s) 801
Psp5II RGGWCCY 1 cut(s) 133
Psp6I CCWGG 2 cut(s) 340, 842
PspGI CCWGG 2 cut(s) 340, 842
PspN4I GGNNCC 3 cut(s) 215, 747, 1160
PspPI GGNCC 3 cut(s) 133, 214, 1145
PspPPI RGGWCCY 1 cut(s) 133
PsuI RGATCY 3 cut(s) 261, 1105, 1158
PvuI CGATCG 1 cut(s) 165
PvuII CAGCTG 1 cut(s) 731
RsaI GTAC 2 cut(s) 679, 722
RsaNI GTAC 2 cut(s) 678, 721
SacII CCGCGG 1 cut(s) 298
SaqAI TTAA 4 cut(s) 497, 609, 801, 822
SatI GCNGC 5 cut(s) 159, 295, 451, 671, 1212
Sau96I GGNCC 3 cut(s) 133, 214, 1145
ScrFI CCNGG 6 cut(s) 15, 342, 407, 408, 844, 1044
SfaNI GCATC 3 cut(s) 55, 259, 847
Sfr303I CCGCGG 1 cut(s) 298
SfuI TTCGAA 1 cut(s) 533
SgrAI CRCCGGYG 1 cut(s) 1378
SgrBI CCGCGG 1 cut(s) 298
SinI GGWCC 2 cut(s) 133, 214
SmaI CCCGGG 1 cut(s) 408
SmlI CTYRAG 1 cut(s) 1109
SmoI CTYRAG 1 cut(s) 1109
Sse9I AATT 9 cut(s) 58, 88, 380, 520, 709, 798, 1080, 1220, 1238
SspI AATATT 2 cut(s) 538, 1354
SspMI CTAG 3 cut(s) 447, 675, 1272
StyD4I CCNGG 6 cut(s) 13, 340, 405, 406, 842, 1042
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 2 cut(s) 886, 1062
TaiI ACGT 2 cut(s) 662, 769
TasI AATT 9 cut(s) 58, 88, 380, 520, 709, 798, 1080, 1220, 1238
TatI WGTACW 1 cut(s) 677
TauI GCSGC 4 cut(s) 161, 297, 453, 673
TfiI GAWTC 5 cut(s) 256, 839, 986, 1055, 1369
Tru1I TTAA 4 cut(s) 497, 609, 801, 822
Tru9I TTAA 4 cut(s) 497, 609, 801, 822
TscAI CASTG 2 cut(s) 1144, 1288
TseFI GTSAC 1 cut(s) 17
TseI GCWGC 1 cut(s) 1211
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 4 cut(s) 393, 794, 1068, 1316
TspGWI ACGGA 3 cut(s) 61, 602, 963
TspMI CCCGGG 1 cut(s) 406
TspRI CASTG 2 cut(s) 1144, 1288
VpaK11BI GGWCC 2 cut(s) 133, 214
VspI ATTAAT 1 cut(s) 801
XagI CCTNNNNNAGG 2 cut(s) 140, 1272
XapI RAATTY 4 cut(s) 58, 88, 380, 709
XbaI TCTAGA 1 cut(s) 1271
XmaI CCCGGG 1 cut(s) 406
XmiI GTMKAC 2 cut(s) 506, 698
XspI CTAG 3 cut(s) 447, 675, 1272
ZraI GACGTC 1 cut(s) 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.