Rmu_sc0008149.1_g000009

Nuclear protein Es2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008149.1
OG0010388
Physical Location & Seq
Reverse (-)
34514 .. 35290
777 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008149.1_g000009.1.cds

Sequence Viewer

Length: 777 bp
atgtaccatcctgctgatcggggtgaggcgccgttgacgttggaggaaaggtcgctgaggatgaagagcttggagaaggagattagtcggaccaatacaaggtttcatgggaagatgatggataggccgaaagaggaaggtggatttgaggtgctttatcctccggttgcaggggggacccctgttatattggatagagatggggataagttgaagaagtatgatttggatgatttgaggaagactccgaatccgttttatgtggaatcagagaagaaagcggacaatgggtatagttttgtgaagacaccgtcccctgcaccaggtgttgatgaatcgccgtttataacatggggtgaaattgaagggacgccactgaggttggaccttgaggacactcctcttgatattggcgggagtgaaggacctcagtataggatcccatgcccggcttcaagagacgagaaggcttactcactttcaagagtggctgcacggaaactgagggaaagggaaagattatttccaaagccgccactgccatcgccttctagagctggcagtgccagtccaagtgtgcggacactctctcctgctgcccagaagtttgtaaggaatgcaattgccaagtcttcatctactgttgatgaaactcttcgagccagttaccgaggtgcaagtcctggtctggctactcctaaagggggaaggagtgtgtcaaggtttagtactatgaaatcaagatcaccttctgcaagggagggctcaccttggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Es2

Protein Analysis

258

Amino Acids

28.44

Weight (kDa)

9.49

Isoelectric Point (pI)

59.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 347
AccB1I GGYRCC 1 cut(s) 28
AciI CCGC 4 cut(s) 281, 414, 533, 580
AclWI GGATC 2 cut(s) 433, 446
AcyI GRCGYC 2 cut(s) 29, 371
AdeI CACNNNGTG 1 cut(s) 326
AfaI GTAC 2 cut(s) 5, 730
AfiI CCNNNNNNNGG 5 cut(s) 99, 170, 323, 448, 704
AgsI TTSAA 4 cut(s) 214, 365, 456, 483
AjnI CCWGG 2 cut(s) 322, 682
AjuI GAANNNNNNNTTGG 2 cut(s) 209, 241
AluBI AGCT 2 cut(s) 69, 557
AluI AGCT 2 cut(s) 69, 557
Alw26I GTCTC 1 cut(s) 453
AlwI GGATC 2 cut(s) 433, 446
AoxI GGCC 1 cut(s) 125
ApeKI GCWGC 2 cut(s) 491, 596
Asp700I GAANNNNTTC 1 cut(s) 654
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 4 cut(s) 90, 177, 385, 425
AsuC2I CCSGG 1 cut(s) 449
AsuHPI GGTGA 4 cut(s) 35, 368, 738, 759
AvaII GGWCC 4 cut(s) 90, 177, 385, 425
BamHI GGATCC 1 cut(s) 438
BanI GGYRCC 1 cut(s) 28
BanII GRGCYC 1 cut(s) 767
BbsI GAAGAC 3 cut(s) 248, 311, 624
BbvCI CCTCAGC 1 cut(s) 56
BbvI GCAGC 2 cut(s) 478, 583
BccI CCATC 4 cut(s) 15, 112, 194, 550
BceAI ACGGC 2 cut(s) 16, 325
BciT130I CCWGG 2 cut(s) 324, 684
BcnI CCSGG 1 cut(s) 449
BcoDI GTCTC 1 cut(s) 453
BfaI CTAG 1 cut(s) 552
BfoI RGCGCY 1 cut(s) 32
BisI GCNGC 3 cut(s) 492, 533, 597
BlsI GCNGC 3 cut(s) 493, 534, 598
BmcAI AGTACT 1 cut(s) 730
Bme1390I CCNGG 3 cut(s) 324, 449, 684
Bme18I GGWCC 4 cut(s) 90, 177, 385, 425
BmgT120I GGNCC 4 cut(s) 90, 177, 385, 425
BmiI GGNNCC 4 cut(s) 30, 178, 179, 440
BmrFI CCNGG 3 cut(s) 324, 449, 684
BpiI GAAGAC 3 cut(s) 248, 311, 624
BplI GAGNNNNNCTC 2 cut(s) 229, 261
Bpu10I CCTNAGC 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 410
BpuMI CCSGG 1 cut(s) 449
BsaHI GRCGYC 2 cut(s) 29, 371
BsaJI CCNNGG 2 cut(s) 670, 770
BsaWI WCCGGW 1 cut(s) 163
BsaXI ACNNNNNCTCC 4 cut(s) 35, 65, 574, 604
Bsc4I CCNNNNNNNGG 5 cut(s) 99, 170, 323, 448, 704
Bse1I ACTGG 2 cut(s) 567, 663
BseBI CCWGG 2 cut(s) 324, 684
BseDI CCNNGG 2 cut(s) 670, 770
BseGI GGATG 3 cut(s) 7, 66, 235
BseLI CCNNNNNNNGG 5 cut(s) 99, 170, 323, 448, 704
BseMII CTCAG 4 cut(s) 47, 368, 443, 494
BseNI ACTGG 2 cut(s) 567, 663
BseRI GAGGAG 1 cut(s) 390
BseXI GCAGC 2 cut(s) 478, 583
BsgI GTGCAG 2 cut(s) 303, 477
BshFI GGCC 1 cut(s) 127
BshNI GGYRCC 1 cut(s) 28
BsiSI CCGG 2 cut(s) 164, 449
BslFI GGGAC 3 cut(s) 190, 298, 382
BslI CCNNNNNNNGG 5 cut(s) 99, 170, 323, 448, 704
BsmAI GTCTC 1 cut(s) 453
BsmBI CGTCTC 1 cut(s) 453
BsmFI GGGAC 3 cut(s) 190, 298, 382
BsmI GAATGC 1 cut(s) 622
BsnI GGCC 1 cut(s) 127
Bsp1286I GDGCHC 1 cut(s) 767
Bsp143I GATC 3 cut(s) 16, 438, 743
BspACI CCGC 4 cut(s) 281, 414, 533, 580
BspANI GGCC 1 cut(s) 127
BspCNI CTCAG 4 cut(s) 48, 369, 442, 495
BspLI GGNNCC 4 cut(s) 30, 178, 179, 440
BspPI GGATC 2 cut(s) 433, 446
BspQI GCTCTTC 1 cut(s) 59
BspT107I GGYRCC 1 cut(s) 28
BsrI ACTGG 2 cut(s) 567, 663
BssECI CCNNGG 2 cut(s) 670, 770
BssMI GATC 3 cut(s) 16, 438, 743
BssNI GRCGYC 2 cut(s) 29, 371
BssT1I CCWWGG 1 cut(s) 770
Bst2UI CCWGG 2 cut(s) 324, 684
Bst4CI ACNGT 2 cut(s) 312, 643
Bst6I CTCTTC 2 cut(s) 59, 660
BstACI GRCGYC 2 cut(s) 29, 371
BstC8I GCNNGC 1 cut(s) 559
BstDEI CTNAG 4 cut(s) 56, 377, 429, 503
BstENI CCTNNNNNAGG 1 cut(s) 321
BstF5I GGATG 3 cut(s) 7, 66, 235
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 3 cut(s) 19, 441, 746
BstMAI GTCTC 1 cut(s) 453
BstMBI GATC 3 cut(s) 16, 438, 743
BstMWI GCNNNNNNNGC 2 cut(s) 538, 563
BstNI CCWGG 2 cut(s) 324, 684
BstSCI CCNGG 3 cut(s) 322, 447, 682
BstV1I GCAGC 2 cut(s) 478, 583
BstV2I GAAGAC 3 cut(s) 248, 311, 624
BstX2I RGATCY 1 cut(s) 438
BstYI RGATCY 1 cut(s) 438
BsuRI GGCC 1 cut(s) 127
BtgZI GCGATG 1 cut(s) 528
BtsCI GGATG 3 cut(s) 7, 66, 235
BtsI GCAGTG 2 cut(s) 536, 568
BtsIMutI CAGTG 3 cut(s) 374, 536, 568
Cac8I GCNNGC 1 cut(s) 559
CfoI GCGC 1 cut(s) 31
Cfr13I GGNCC 4 cut(s) 90, 177, 385, 425
CseI GACGC 1 cut(s) 379
CsiI ACCWGGT 1 cut(s) 322
Csp6I GTAC 2 cut(s) 4, 729
CspCI CAANNNNNGTGG 2 cut(s) 363, 398
CviAII CATG 3 cut(s) 107, 351, 444
CviQI GTAC 2 cut(s) 4, 729
DdeI CTNAG 4 cut(s) 56, 377, 429, 503
DinI GGCGCC 1 cut(s) 30
DpnI GATC 3 cut(s) 18, 440, 745
DpnII GATC 3 cut(s) 16, 438, 743
DraIII CACNNNGTG 1 cut(s) 326
Eam1104I CTCTTC 2 cut(s) 59, 660
EarI CTCTTC 2 cut(s) 59, 660
Eco130I CCWWGG 1 cut(s) 770
Eco24I GRGCYC 1 cut(s) 767
Eco47I GGWCC 4 cut(s) 90, 177, 385, 425
EcoNI CCTNNNNNAGG 1 cut(s) 321
EcoO109I RGGNCCY 2 cut(s) 177, 425
EcoRII CCWGG 2 cut(s) 322, 682
EcoT14I CCWWGG 1 cut(s) 770
EcoT38I GRGCYC 1 cut(s) 767
EgeI GGCGCC 1 cut(s) 30
EheI GGCGCC 1 cut(s) 30
ErhI CCWWGG 1 cut(s) 770
Esp3I CGTCTC 1 cut(s) 453
FaeI CATG 3 cut(s) 110, 354, 447
FalI AAGNNNNNCTT 2 cut(s) 733, 765
FaqI GGGAC 3 cut(s) 190, 298, 382
FatI CATG 3 cut(s) 106, 350, 443
FauI CCCGC 1 cut(s) 407
Fnu4HI GCNGC 3 cut(s) 492, 533, 597
FokI GGATG 2 cut(s) 73, 242
FriOI GRGCYC 1 cut(s) 767
Fsp4HI GCNGC 3 cut(s) 492, 533, 597
FspBI CTAG 1 cut(s) 552
GlaI GCGC 1 cut(s) 30
GluI GCNGC 3 cut(s) 492, 533, 597
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 1 cut(s) 127
HapII CCGG 2 cut(s) 164, 449
HgaI GACGC 1 cut(s) 379
HhaI GCGC 1 cut(s) 31
Hin1I GRCGYC 2 cut(s) 29, 371
Hin1II CATG 3 cut(s) 110, 354, 447
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HincII GTYRAC 1 cut(s) 36
HindII GTYRAC 1 cut(s) 36
HinfI GANTC 4 cut(s) 244, 250, 266, 335
HpaII CCGG 2 cut(s) 164, 449
HphI GGTGA 4 cut(s) 35, 368, 738, 759
Hpy166II GTNNAC 1 cut(s) 36
Hpy188I TCNGA 3 cut(s) 90, 249, 271
Hpy188III TCNNGA 5 cut(s) 404, 456, 483, 552, 741
Hpy8I GTNNAC 1 cut(s) 36
HpyAV CCTTC 8 cut(s) 70, 131, 359, 416, 460, 558, 702, 759
HpyCH4III ACNGT 2 cut(s) 312, 643
HpyCH4IV ACGT 1 cut(s) 38
HpyCH4V TGCA 6 cut(s) 170, 320, 494, 620, 677, 755
HpyF10VI GCNNNNNNNGC 2 cut(s) 538, 563
HpyF3I CTNAG 4 cut(s) 56, 377, 429, 503
HpySE526I ACGT 1 cut(s) 38
Hsp92I GRCGYC 2 cut(s) 29, 371
Hsp92II CATG 3 cut(s) 110, 354, 447
HspAI GCGC 1 cut(s) 29
KasI GGCGCC 1 cut(s) 28
KflI GGGWCCC 1 cut(s) 177
Kzo9I GATC 3 cut(s) 16, 438, 743
LguI GCTCTTC 1 cut(s) 59
Lsp1109I GCAGC 2 cut(s) 478, 583
MabI ACCWGGT 1 cut(s) 322
MaeI CTAG 1 cut(s) 552
MaeII ACGT 1 cut(s) 38
MaeIII GTNAC 1 cut(s) 665
MalI GATC 3 cut(s) 18, 440, 745
MboI GATC 3 cut(s) 16, 438, 743
MboII GAAGA 8 cut(s) 76, 124, 226, 253, 286, 316, 624, 647
MfeI CAATTG 1 cut(s) 621
MflI RGATCY 1 cut(s) 438
MhlI GDGCHC 1 cut(s) 767
MluCI AATT 2 cut(s) 360, 621
Mly113I GGCGCC 1 cut(s) 29
MlyI GAGTC 1 cut(s) 238
MmeI TCCRAC 3 cut(s) 21, 68, 363
MroXI GAANNNNTTC 1 cut(s) 654
MspI CCGG 2 cut(s) 164, 449
MspR9I CCNGG 3 cut(s) 324, 449, 684
MunI CAATTG 1 cut(s) 621
Mva1269I GAATGC 1 cut(s) 622
MvaI CCWGG 2 cut(s) 324, 684
MwoI GCNNNNNNNGC 2 cut(s) 538, 563
NarI GGCGCC 1 cut(s) 29
NciI CCSGG 1 cut(s) 449
NdeII GATC 3 cut(s) 16, 438, 743
NlaIII CATG 3 cut(s) 110, 354, 447
NlaIV GGNNCC 4 cut(s) 30, 178, 179, 440
PciSI GCTCTTC 1 cut(s) 59
PctI GAATGC 1 cut(s) 622
PdmI GAANNNNTTC 1 cut(s) 654
PfeI GAWTC 3 cut(s) 250, 266, 335
PflFI GACNNNGTC 1 cut(s) 310
PkrI GCNGC 3 cut(s) 493, 534, 598
PleI GAGTC 1 cut(s) 238
PluTI GGCGCC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 238
PpuMI RGGWCCY 2 cut(s) 177, 425
PsiI TTATAA 1 cut(s) 347
Psp5II RGGWCCY 2 cut(s) 177, 425
Psp6I CCWGG 2 cut(s) 322, 682
PspGI CCWGG 2 cut(s) 322, 682
PspN4I GGNNCC 4 cut(s) 30, 178, 179, 440
PspPI GGNCC 4 cut(s) 90, 177, 385, 425
PspPPI RGGWCCY 2 cut(s) 177, 425
PsuI RGATCY 1 cut(s) 438
PsyI GACNNNGTC 1 cut(s) 310
RsaI GTAC 2 cut(s) 5, 730
RsaNI GTAC 2 cut(s) 4, 729
SapI GCTCTTC 1 cut(s) 59
SatI GCNGC 3 cut(s) 492, 533, 597
Sau3AI GATC 3 cut(s) 16, 438, 743
Sau96I GGNCC 4 cut(s) 90, 177, 385, 425
ScaI AGTACT 1 cut(s) 730
SchI GAGTC 1 cut(s) 238
ScrFI CCNGG 3 cut(s) 324, 449, 684
SduI GDGCHC 1 cut(s) 767
SexAI ACCWGGT 1 cut(s) 322
SfoI GGCGCC 1 cut(s) 30
SinI GGWCC 4 cut(s) 90, 177, 385, 425
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 2 cut(s) 360, 621
SsiI CCGC 4 cut(s) 281, 414, 533, 580
SspDI GGCGCC 1 cut(s) 28
SspMI CTAG 1 cut(s) 552
StyD4I CCNGG 3 cut(s) 322, 447, 682
StyI CCWWGG 1 cut(s) 770
TaaI ACNGT 2 cut(s) 312, 643
TaiI ACGT 1 cut(s) 41
TaqI TCGA 1 cut(s) 658
TasI AATT 2 cut(s) 360, 621
TatI WGTACW 1 cut(s) 728
TauI GCSGC 1 cut(s) 535
TfiI GAWTC 3 cut(s) 250, 266, 335
TscAI CASTG 3 cut(s) 381, 543, 568
TseI GCWGC 2 cut(s) 491, 596
TspDTI ATGAA 6 cut(s) 77, 95, 348, 624, 663, 749
TspGWI ACGGA 2 cut(s) 243, 511
TspRI CASTG 3 cut(s) 381, 543, 568
Tth111I GACNNNGTC 1 cut(s) 310
VpaK11BI GGWCC 4 cut(s) 90, 177, 385, 425
XagI CCTNNNNNAGG 1 cut(s) 321
XbaI TCTAGA 1 cut(s) 551
XmnI GAANNNNTTC 1 cut(s) 654
XspI CTAG 1 cut(s) 552
ZrmI AGTACT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.