MD17G1163100.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0017915
Physical Location & Seq
Reverse (-)
16134561 .. 16139919
5359 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1163100.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGGAAATAGTATGGAGATTTAGGTATTGTTTTGTGCCCAGAGGGCCAACCCAAATCATCAGAGCACTGCGCATGCTGCCTATCTGCCACTTTTCATCACCAAAGATGTGTCCAACTAACAAAACCCAAGAAAATTTCCCGAAACTTTTTGACCGACAAACAAGTAAACTCAATCATCAAGTTGTGCATTCCACTTTATTGAACTGCCCTTCTGATTTGATTGCATTGAGATTCTTCTTGTGGTGTGCCGGACAACATAATTTTTTTCACAGTAAGATTGTGATTGATCACATGGTTGGTGTGATCAAGCGCGTGATGGAACAATACAGAACCGTTAAATTGGTTATTAGGGAACTAGAGAGCATGGGGTGTGTTGTAAAATCTCAAACATTTTTGCTGTTGTTGAGGATTTATTGGCGTGGCGAAATGTATACAATGGTCTTTGAGGCTATTGAGCTAATGGGTACTTATGGCTTTACTCCAAACACATTTGCTCGTAACATAATCATTGATGCGTTGTTCAAAATTGGGCATGTGGATTTAGCTATTAAGTTTCTGAAAGAGACCCGAAGGCCAAATTTTTTGACTTTTAACATTGCACTGTGTAATTTATGTAATATCAATGATTTATATCACATTGGAGATGTGTTTAGAATGATGTTGCGATGGGGATATCACCCCAAAGTGGAGACATTTGAGATGATTTTGAGCTGTTTATGCAAAATGGGTAAAATAGTGGAGGCGCATCAAGTTTTGGGTCTATTGATTACTTTGGGTAGCGCTGTGTCTGTAAATGTTTGGAGTATGTTGATGAATGGGTTTTGTCGGTTGCAGAGACTTGATGTGGCTGGTAAGTTATTGGAGAAGATGGTTGAGACTGGTTCTTCACCTAGTATTGTAACGTACACAACTTTAATCAGGGGATTTTTAAAATCTAATATGGTTAGTGAAGCATTCAATATTCTAAATATTATGGAATCCAAAGGATATGCCCTTGACCTCGTTCTTTGTAATGTGTTAATAGATTCCTTTGCCAAAGCTGGGAGGTGTGATGATGCCATTGATGTTTTTGTTAGCATGCAATCACGGAACTTGGCTCCTGATTCTTGTACTGTCTCTTCATTATTATCCACCTTGTGTTTGTCTAGGAGGTTTGATATGTTACCCAAGTTGGTTCGTGGACTTGACATAGAAGCTGACTTATTGCTATGA

Protein Analysis

404

Amino Acids

46.13

Weight (kDa)

8.99

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 264 - 311 4.4e-11 PPR repeat family
PPR_1 PF12854 294 - 326 9.2e-07 PPR repeat
PPR_2 PF13041 297 - 333 2.8e-08 PPR repeat family
PPR PF01535 300 - 329 2.1e-06 PPR repeat
PPR_2 PF13041 333 - 380 4.9e-11 PPR repeat family
PPR PF01535 335 - 364 7.9e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 71
AccI GTMKAC 1 cut(s) 431
AccII CGCG 1 cut(s) 312
AcsI RAATTY 2 cut(s) 133, 577
AdeI CACNNNGTG 1 cut(s) 1137
AfaI GTAC 3 cut(s) 466, 905, 1111
AfeI AGCGCT 1 cut(s) 781
AfiI CCNNNNNNNGG 2 cut(s) 685, 1041
AgsI TTSAA 3 cut(s) 202, 523, 958
AluBI AGCT 5 cut(s) 457, 545, 711, 1040, 1196
AluI AGCT 5 cut(s) 457, 545, 711, 1040, 1196
Alw21I GWGCWC 1 cut(s) 67
Alw26I GTCTC 5 cut(s) 557, 683, 829, 869, 1120
Aor51HI AGCGCT 1 cut(s) 781
AoxI GGCC 2 cut(s) 44, 572
ApeKI GCWGC 1 cut(s) 76
ApoI RAATTY 2 cut(s) 133, 577
AspLEI GCGC 4 cut(s) 72, 312, 745, 782
AspS9I GGNCC 1 cut(s) 44
AsuHPI GGTGA 3 cut(s) 90, 668, 879
BaeGI GKGCMC 1 cut(s) 39
BarI GAAGNNNNNNTAC 2 cut(s) 1102, 1134
Bbv12I GWGCWC 1 cut(s) 67
BbvI GCAGC 1 cut(s) 63
BccI CCATC 3 cut(s) 310, 660, 862
BclI TGATCA 2 cut(s) 286, 303
BcoDI GTCTC 5 cut(s) 557, 683, 829, 869, 1120
BfaI CTAG 3 cut(s) 356, 891, 1146
BfoI RGCGCY 1 cut(s) 783
BglI GCCNNNNNGGC 1 cut(s) 43
BisI GCNGC 1 cut(s) 77
BlsI GCNGC 1 cut(s) 78
BmgT120I GGNCC 1 cut(s) 44
BmiI GGNNCC 1 cut(s) 1098
BmsI GCATC 3 cut(s) 502, 754, 1045
BsaBI GATNNNNATC 1 cut(s) 630
BsaI GGTCTC 1 cut(s) 557
Bsc4I CCNNNNNNNGG 2 cut(s) 685, 1041
Bse1I ACTGG 1 cut(s) 883
Bse3DI GCAATG 1 cut(s) 594
Bse8I GATNNNNATC 1 cut(s) 630
BseJI GATNNNNATC 1 cut(s) 630
BseLI CCNNNNNNNGG 2 cut(s) 685, 1041
BseMI GCAATG 1 cut(s) 594
BseNI ACTGG 1 cut(s) 883
BseSI GKGCMC 1 cut(s) 39
BseXI GCAGC 1 cut(s) 63
BseYI CCCAGC 1 cut(s) 1040
Bsh1236I CGCG 1 cut(s) 312
BshFI GGCC 2 cut(s) 46, 574
BsiHKAI GWGCWC 1 cut(s) 67
BsiSI CCGG 1 cut(s) 249
BslI CCNNNNNNNGG 2 cut(s) 685, 1041
BsmAI GTCTC 5 cut(s) 557, 683, 829, 869, 1120
BsmI GAATGC 2 cut(s) 187, 953
BsnI GGCC 2 cut(s) 46, 574
Bso31I GGTCTC 1 cut(s) 557
Bsp1286I GDGCHC 2 cut(s) 39, 67
Bsp143I GATC 2 cut(s) 286, 303
BspANI GGCC 2 cut(s) 46, 574
BspFNI CGCG 1 cut(s) 312
BspLI GGNNCC 1 cut(s) 1098
BspTNI GGTCTC 1 cut(s) 557
BsrDI GCAATG 1 cut(s) 594
BsrI ACTGG 1 cut(s) 883
BssMI GATC 2 cut(s) 286, 303
BssNAI GTATAC 1 cut(s) 432
Bst1107I GTATAC 1 cut(s) 432
Bst4CI ACNGT 4 cut(s) 272, 334, 603, 1114
Bst6I CTCTTC 1 cut(s) 1123
BstC8I GCNNGC 2 cut(s) 74, 1079
BstFNI CGCG 1 cut(s) 312
BstH2I RGCGCY 1 cut(s) 783
BstHHI GCGC 4 cut(s) 72, 312, 745, 782
BstKTI GATC 2 cut(s) 289, 306
BstMAI GTCTC 5 cut(s) 557, 683, 829, 869, 1120
BstMBI GATC 2 cut(s) 286, 303
BstMWI GCNNNNNNNGC 3 cut(s) 43, 76, 717
BstNSI RCATGY 3 cut(s) 76, 536, 1081
BstSLI GKGCMC 1 cut(s) 39
BstUI CGCG 1 cut(s) 312
BstV1I GCAGC 1 cut(s) 63
BstZ17I GTATAC 1 cut(s) 432
BsuRI GGCC 2 cut(s) 46, 574
BtgZI GCGATG 1 cut(s) 679
BtsI GCAGTG 1 cut(s) 65
BtsIMutI CAGTG 2 cut(s) 65, 599
Cac8I GCNNGC 2 cut(s) 74, 1079
CfoI GCGC 4 cut(s) 72, 312, 745, 782
Cfr13I GGNCC 1 cut(s) 44
Csp6I GTAC 3 cut(s) 465, 904, 1110
CviAII CATG 5 cut(s) 73, 292, 364, 533, 1078
CviQI GTAC 3 cut(s) 465, 904, 1110
DpnI GATC 2 cut(s) 288, 305
DpnII GATC 2 cut(s) 286, 303
DraI TTTAAA 1 cut(s) 930
DraIII CACNNNGTG 1 cut(s) 1137
Eam1104I CTCTTC 1 cut(s) 1123
EarI CTCTTC 1 cut(s) 1123
Eco31I GGTCTC 1 cut(s) 557
Eco32I GATATC 1 cut(s) 674
Eco47III AGCGCT 1 cut(s) 781
EcoRV GATATC 1 cut(s) 674
FaeI CATG 5 cut(s) 76, 295, 367, 536, 1081
FatI CATG 5 cut(s) 72, 291, 363, 532, 1077
FbaI TGATCA 2 cut(s) 286, 303
FblI GTMKAC 1 cut(s) 431
Fnu4HI GCNGC 1 cut(s) 77
Fsp4HI GCNGC 1 cut(s) 77
FspBI CTAG 3 cut(s) 356, 891, 1146
FspI TGCGCA 1 cut(s) 71
GlaI GCGC 4 cut(s) 71, 311, 744, 781
GluI GCNGC 1 cut(s) 77
GsaI CCCAGC 1 cut(s) 1044
HaeII RGCGCY 1 cut(s) 783
HaeIII GGCC 2 cut(s) 46, 574
HapII CCGG 1 cut(s) 249
HhaI GCGC 4 cut(s) 72, 312, 745, 782
Hin1II CATG 5 cut(s) 76, 295, 367, 536, 1081
Hin6I GCGC 4 cut(s) 70, 310, 743, 780
HinP1I GCGC 4 cut(s) 70, 310, 743, 780
HinfI GANTC 4 cut(s) 231, 977, 1025, 1103
HpaII CCGG 1 cut(s) 249
HphI GGTGA 3 cut(s) 90, 668, 879
Hpy166II GTNNAC 4 cut(s) 167, 432, 906, 1181
Hpy188I TCNGA 3 cut(s) 62, 214, 558
Hpy188III TCNNGA 2 cut(s) 139, 1100
Hpy8I GTNNAC 4 cut(s) 167, 432, 906, 1181
HpyAV CCTTC 2 cut(s) 219, 564
HpyCH4III ACNGT 4 cut(s) 272, 334, 603, 1114
HpyCH4IV ACGT 1 cut(s) 902
HpyCH4V TGCA 6 cut(s) 187, 224, 599, 720, 832, 1081
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 76, 717
HpySE526I ACGT 1 cut(s) 902
Hsp92II CATG 5 cut(s) 76, 295, 367, 536, 1081
HspAI GCGC 4 cut(s) 70, 310, 743, 780
Ksp22I TGATCA 2 cut(s) 286, 303
Kzo9I GATC 2 cut(s) 286, 303
LmnI GCTCC 1 cut(s) 1102
LpnPI CCDG 7 cut(s) 52, 262, 834, 864, 904, 1026, 1113
Lsp1109I GCAGC 1 cut(s) 63
LweI GCATC 3 cut(s) 502, 754, 1045
MaeI CTAG 3 cut(s) 356, 891, 1146
MaeII ACGT 1 cut(s) 902
MaeIII GTNAC 3 cut(s) 497, 898, 1161
MalI GATC 2 cut(s) 288, 305
MboI GATC 2 cut(s) 286, 303
MboII GAAGA 4 cut(s) 226, 876, 877, 1110
MhlI GDGCHC 2 cut(s) 39, 67
MluCI AATT 6 cut(s) 133, 259, 338, 525, 577, 607
MmeI TCCRAC 1 cut(s) 137
MnlI CCTC 7 cut(s) 35, 399, 439, 733, 1010, 1038, 1143
MseI TTAA 6 cut(s) 336, 549, 591, 914, 929, 1019
MspI CCGG 1 cut(s) 249
Mva1269I GAATGC 2 cut(s) 187, 953
MvnI CGCG 1 cut(s) 312
MwoI GCNNNNNNNGC 3 cut(s) 43, 76, 717
NdeII GATC 2 cut(s) 286, 303
NlaIII CATG 5 cut(s) 76, 295, 367, 536, 1081
NlaIV GGNNCC 1 cut(s) 1098
NsbI TGCGCA 1 cut(s) 71
NspI RCATGY 3 cut(s) 76, 536, 1081
PaeI GCATGC 2 cut(s) 76, 1081
PctI GAATGC 2 cut(s) 187, 953
PfeI GAWTC 4 cut(s) 231, 977, 1025, 1103
PkrI GCNGC 1 cut(s) 78
PspFI CCCAGC 1 cut(s) 1040
PspN4I GGNNCC 1 cut(s) 1098
PspPI GGNCC 1 cut(s) 44
RsaI GTAC 3 cut(s) 466, 905, 1111
RsaNI GTAC 3 cut(s) 465, 904, 1110
SaqAI TTAA 6 cut(s) 336, 549, 591, 914, 929, 1019
SatI GCNGC 1 cut(s) 77
Sau3AI GATC 2 cut(s) 286, 303
Sau96I GGNCC 1 cut(s) 44
SduI GDGCHC 2 cut(s) 39, 67
SfaNI GCATC 3 cut(s) 502, 754, 1045
SphI GCATGC 2 cut(s) 76, 1081
Sse9I AATT 6 cut(s) 133, 259, 338, 525, 577, 607
SspI AATATT 2 cut(s) 961, 970
SspMI CTAG 3 cut(s) 356, 891, 1146
TaaI ACNGT 4 cut(s) 272, 334, 603, 1114
TaiI ACGT 1 cut(s) 905
TaqII GACCGA 1 cut(s) 168
TasI AATT 6 cut(s) 133, 259, 338, 525, 577, 607
TatI WGTACW 1 cut(s) 1109
TfiI GAWTC 4 cut(s) 231, 977, 1025, 1103
Tru1I TTAA 6 cut(s) 336, 549, 591, 914, 929, 1019
Tru9I TTAA 6 cut(s) 336, 549, 591, 914, 929, 1019
TscAI CASTG 2 cut(s) 72, 606
TseI GCWGC 1 cut(s) 76
TspDTI ATGAA 3 cut(s) 84, 827, 1110
TspGWI ACGGA 1 cut(s) 1102
TspRI CASTG 2 cut(s) 72, 606
XapI RAATTY 2 cut(s) 133, 577
XceI RCATGY 3 cut(s) 76, 536, 1081
XmiI GTMKAC 1 cut(s) 431
XspI CTAG 3 cut(s) 356, 891, 1146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.