MD17G1163200.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0017915
Physical Location & Seq
Reverse (-)
16141515 .. 16144956
3442 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1163200.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGATGAGTGGCAAGCAGATGAAAAGACTTGATGTGGCTGGTAAGTTATTGGAGAAGATGGTTGAGACTGGTTCTTCTCCTAGTATTGTAACGTACACGACTTTAATCAGGGGATTTTTAAAATCTAATATGGTTAGTGAAGCATTCAATATTCTAAATATTATGGAATCCAAAGGATATGCCCCTGACCTCGTTCTTTGTAATGTGTTAATAGACTCCTTTGCCAAAGCTGGGAGGTGTGATGATGCCATTAATGTTTTTGTTAGCATGCGATCACGGAACTTGGCTCCTGATTCTTGTACTGTCTCTTCATTATTATCTACCTTGTGTTTGTCTAGGAGGTTTGATATGTTACCCAAGTTGGTTCGTGGACTTGACATAGAAGCTGACTTATTGCTATGTAACTCACTTCTCTGTTATTTTTGTAAAGCCAAGTTTCCGTCTCTTGCTGTAAAGTTTTATAATGATATGCTTGATAATGGCCTTACCCCAGATAAGTATACTTTTGTTGGATTAGTAGATGGGTTATGCAAGGCAAAAAGAGTTGATGAAGCAGTTGATGCGTATCATGGGATTCTCCAGAGTTTCCCTGGACAGGATGCTTATATTCATACTGTGGTCATGGATGGGCTTATAAAGGTCCGTAAATTTAATGCGGCCATTAGAGTGTTTAGAAAAGCAGTTGAAGAGGGCTACACCCTTGATGTTGCAGCATACACAGTTGCCATTATAGGGCTCTTTATGGGTGGTAGAGCTGGAGAGGCTTGGTCACTCTATTGCCAGATGAAGGAAGTCGGCTTGGCTCTTACTGTACATACATACAATGTAATGGTCTCTGGTTTTGTTAAAGAAAGAGATCTTAATATGGTTAATTTACTGCTACAAGAGATGATAGAAGCAAAAGTAGAACTGAGCTCCAATACTTTCTTGAGATTATCAAAATTCCTCTGTAGACCATATCTTTCTAGTTCAGTTATTGAACTATGGATTGAGATGAGAAGTTTGGGTTTGATATCTTCTAAAGTAGTGCAAGAATTACTTTCTGATGAAGTTGCCAAAGGCATGAAAGTAGATGATGGCCTAATTGCAATTTCAGACGTTACTTCAGAAACTGATCGATCTGTGGAGGCATCTGGCTCTGAAGACGTTTATGATGTGGCTGCTTCAATGGCTTGA

Protein Analysis

392

Amino Acids

43.12

Weight (kDa)

5.48

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 7 - 41 3.1e-06 PPR repeat family
PPR_long PF17177 10 - 107 2.2e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 15 - 72 5.5e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 24 - 56 8.9e-07 PPR repeat
PPR_2 PF13041 27 - 64 1.5e-09 PPR repeat family
PPR PF01535 30 - 59 2e-06 PPR repeat
PPR_2 PF13041 62 - 110 1.7e-11 PPR repeat family
PPR_2 PF13041 133 - 178 3e-12 PPR repeat family
PPR_1 PF12854 160 - 186 6.6e-07 PPR repeat
PPR_2 PF13041 235 - 283 1.6e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 462, 635
AccI GTMKAC 2 cut(s) 500, 952
AciI CCGC 1 cut(s) 656
AcoI YGGCCR 1 cut(s) 657
AcsI RAATTY 2 cut(s) 647, 941
AcuI CTGAAG 2 cut(s) 1089, 1161
AfaI GTAC 3 cut(s) 95, 301, 813
AfiI CCNNNNNNNGG 4 cut(s) 231, 595, 732, 787
AgsI TTSAA 4 cut(s) 148, 686, 980, 1167
AjnI CCWGG 1 cut(s) 589
AluBI AGCT 4 cut(s) 230, 386, 755, 915
AluI AGCT 4 cut(s) 230, 386, 755, 915
Alw21I GWGCWC 1 cut(s) 917
Alw26I GTCTC 4 cut(s) 59, 310, 447, 838
AlwNI CAGNNNCTG 1 cut(s) 1112
AoxI GGCC 3 cut(s) 481, 657, 1078
ApeKI GCWGC 2 cut(s) 710, 1160
ApoI RAATTY 2 cut(s) 647, 941
AseI ATTAAT 1 cut(s) 252
AspS9I GGNCC 1 cut(s) 640
AvaII GGWCC 1 cut(s) 640
BanII GRGCYC 2 cut(s) 738, 917
BarI GAAGNNNNNNTAC 2 cut(s) 292, 324
BbsI GAAGAC 1 cut(s) 1149
Bbv12I GWGCWC 1 cut(s) 917
BbvI GCAGC 2 cut(s) 722, 1147
BccI CCATC 4 cut(s) 52, 515, 620, 1070
BciT130I CCWGG 1 cut(s) 591
BcoDI GTCTC 4 cut(s) 59, 310, 447, 838
BfaI CTAG 3 cut(s) 81, 336, 966
BfmI CTRYAG 1 cut(s) 949
BglII AGATCT 1 cut(s) 856
BisI GCNGC 3 cut(s) 657, 711, 1161
BlsI GCNGC 3 cut(s) 658, 712, 1162
Bme1390I CCNGG 1 cut(s) 591
Bme18I GGWCC 1 cut(s) 640
BmgT120I GGNCC 1 cut(s) 640
BmiI GGNNCC 1 cut(s) 288
BmrFI CCNGG 1 cut(s) 591
BmsI GCATC 4 cut(s) 235, 550, 589, 1139
BpiI GAAGAC 1 cut(s) 1149
BpmI CTGGAG 2 cut(s) 563, 777
BpuEI CTTGAG 1 cut(s) 949
Bsa29I ATCGAT 1 cut(s) 1117
BsaBI GATNNNNATC 1 cut(s) 564
BsaI GGTCTC 1 cut(s) 838
BsaJI CCNNGG 1 cut(s) 589
Bsc4I CCNNNNNNNGG 4 cut(s) 231, 595, 732, 787
Bse1I ACTGG 1 cut(s) 73
Bse8I GATNNNNATC 1 cut(s) 564
BseBI CCWGG 1 cut(s) 591
BseCI ATCGAT 1 cut(s) 1117
BseDI CCNNGG 1 cut(s) 589
BseGI GGATG 2 cut(s) 604, 631
BseJI GATNNNNATC 1 cut(s) 564
BseLI CCNNNNNNNGG 4 cut(s) 231, 595, 732, 787
BseMII CTCAG 1 cut(s) 902
BseNI ACTGG 1 cut(s) 73
BseXI GCAGC 2 cut(s) 722, 1147
BseYI CCCAGC 1 cut(s) 230
BshFI GGCC 3 cut(s) 483, 659, 1080
BshVI ATCGAT 1 cut(s) 1117
BsiHKAI GWGCWC 1 cut(s) 917
BslI CCNNNNNNNGG 4 cut(s) 231, 595, 732, 787
BsmAI GTCTC 4 cut(s) 59, 310, 447, 838
BsmBI CGTCTC 1 cut(s) 447
BsmI GAATGC 1 cut(s) 143
BsnI GGCC 3 cut(s) 483, 659, 1080
Bso31I GGTCTC 1 cut(s) 838
Bsp1286I GDGCHC 2 cut(s) 738, 917
Bsp1407I TGTACA 1 cut(s) 811
Bsp143I GATC 4 cut(s) 272, 856, 1114, 1118
BspACI CCGC 1 cut(s) 656
BspANI GGCC 3 cut(s) 483, 659, 1080
BspCNI CTCAG 1 cut(s) 903
BspDI ATCGAT 1 cut(s) 1117
BspLI GGNNCC 1 cut(s) 288
BspTNI GGTCTC 1 cut(s) 838
BsrGI TGTACA 1 cut(s) 811
BsrI ACTGG 1 cut(s) 73
BssECI CCNNGG 1 cut(s) 589
BssMI GATC 4 cut(s) 272, 856, 1114, 1118
BssNAI GTATAC 1 cut(s) 501
Bst1107I GTATAC 1 cut(s) 501
Bst2UI CCWGG 1 cut(s) 591
Bst4CI ACNGT 4 cut(s) 304, 616, 721, 811
Bst6I CTCTTC 2 cut(s) 313, 681
BstAPI GCANNNNNTGC 1 cut(s) 560
BstAUI TGTACA 1 cut(s) 811
BstC8I GCNNGC 2 cut(s) 14, 269
BstDEI CTNAG 1 cut(s) 911
BstF5I GGATG 2 cut(s) 604, 631
BstKTI GATC 4 cut(s) 275, 859, 1117, 1121
BstMAI GTCTC 4 cut(s) 59, 310, 447, 838
BstMBI GATC 4 cut(s) 272, 856, 1114, 1118
BstMWI GCNNNNNNNGC 3 cut(s) 560, 761, 1169
BstNI CCWGG 1 cut(s) 591
BstNSI RCATGY 1 cut(s) 271
BstSCI CCNGG 1 cut(s) 589
BstSFI CTRYAG 1 cut(s) 949
BstV1I GCAGC 2 cut(s) 722, 1147
BstV2I GAAGAC 1 cut(s) 1149
BstX2I RGATCY 1 cut(s) 856
BstYI RGATCY 1 cut(s) 856
BstZ17I GTATAC 1 cut(s) 501
Bsu15I ATCGAT 1 cut(s) 1117
BsuRI GGCC 3 cut(s) 483, 659, 1080
BsuTUI ATCGAT 1 cut(s) 1117
BtsCI GGATG 2 cut(s) 604, 631
Cac8I GCNNGC 2 cut(s) 14, 269
CaiI CAGNNNCTG 1 cut(s) 1112
Cfr13I GGNCC 1 cut(s) 640
ClaI ATCGAT 1 cut(s) 1117
Csp6I GTAC 3 cut(s) 94, 300, 812
CviAII CATG 4 cut(s) 268, 569, 622, 1063
CviQI GTAC 3 cut(s) 94, 300, 812
DdeI CTNAG 1 cut(s) 911
DpnI GATC 4 cut(s) 274, 858, 1116, 1120
DpnII GATC 4 cut(s) 272, 856, 1114, 1118
DraI TTTAAA 1 cut(s) 120
EaeI YGGCCR 1 cut(s) 657
Eam1104I CTCTTC 2 cut(s) 313, 681
EarI CTCTTC 2 cut(s) 313, 681
Ecl136II GAGCTC 1 cut(s) 915
Eco24I GRGCYC 2 cut(s) 738, 917
Eco31I GGTCTC 1 cut(s) 838
Eco32I GATATC 1 cut(s) 1014
Eco47I GGWCC 1 cut(s) 640
Eco53kI GAGCTC 1 cut(s) 915
Eco57I CTGAAG 2 cut(s) 1089, 1161
EcoICRI GAGCTC 1 cut(s) 915
EcoRII CCWGG 1 cut(s) 589
EcoRV GATATC 1 cut(s) 1014
EcoT38I GRGCYC 2 cut(s) 738, 917
Esp3I CGTCTC 1 cut(s) 447
FaeI CATG 4 cut(s) 271, 572, 625, 1066
FalI AAGNNNNNCTT 2 cut(s) 1023, 1055
FatI CATG 4 cut(s) 267, 568, 621, 1062
FblI GTMKAC 2 cut(s) 500, 952
Fnu4HI GCNGC 3 cut(s) 657, 711, 1161
FokI GGATG 2 cut(s) 611, 638
FriOI GRGCYC 2 cut(s) 738, 917
Fsp4HI GCNGC 3 cut(s) 657, 711, 1161
FspBI CTAG 3 cut(s) 81, 336, 966
GluI GCNGC 3 cut(s) 657, 711, 1161
GsaI CCCAGC 1 cut(s) 234
GsuI CTGGAG 2 cut(s) 563, 777
HaeIII GGCC 3 cut(s) 483, 659, 1080
Hin1II CATG 4 cut(s) 271, 572, 625, 1066
HinfI GANTC 4 cut(s) 167, 215, 293, 574
Hpy166II GTNNAC 4 cut(s) 96, 371, 501, 953
Hpy188I TCNGA 4 cut(s) 1045, 1096, 1108, 1141
Hpy188III TCNNGA 3 cut(s) 290, 580, 928
Hpy8I GTNNAC 4 cut(s) 96, 371, 501, 953
HpyAV CCTTC 1 cut(s) 781
HpyCH4III ACNGT 4 cut(s) 304, 616, 721, 811
HpyCH4IV ACGT 3 cut(s) 92, 1098, 1146
HpyCH4V TGCA 4 cut(s) 531, 710, 1030, 1088
HpyF10VI GCNNNNNNNGC 3 cut(s) 560, 761, 1169
HpyF3I CTNAG 1 cut(s) 911
HpySE526I ACGT 3 cut(s) 92, 1098, 1146
Hsp92II CATG 4 cut(s) 271, 572, 625, 1066
Kzo9I GATC 4 cut(s) 272, 856, 1114, 1118
LmnI GCTCC 2 cut(s) 292, 920
Lsp1109I GCAGC 2 cut(s) 722, 1147
LweI GCATC 4 cut(s) 235, 550, 589, 1139
MaeI CTAG 3 cut(s) 81, 336, 966
MaeII ACGT 3 cut(s) 92, 1098, 1146
MaeIII GTNAC 5 cut(s) 88, 351, 401, 768, 1099
MalI GATC 4 cut(s) 274, 858, 1116, 1120
MboI GATC 4 cut(s) 272, 856, 1114, 1118
MboII GAAGA 6 cut(s) 66, 67, 300, 698, 1008, 1154
MflI RGATCY 1 cut(s) 856
MhlI GDGCHC 2 cut(s) 738, 917
MluCI AATT 6 cut(s) 647, 871, 941, 1034, 1083, 1089
MlyI GAGTC 1 cut(s) 209
MmeI TCCRAC 1 cut(s) 490
MnlI CCTC 7 cut(s) 200, 228, 333, 682, 754, 956, 1120
MseI TTAA 8 cut(s) 104, 119, 209, 252, 651, 846, 861, 870
MslI CAYNNNNRTG 1 cut(s) 665
MspR9I CCNGG 1 cut(s) 591
Mva1269I GAATGC 1 cut(s) 143
MvaI CCWGG 1 cut(s) 591
MwoI GCNNNNNNNGC 3 cut(s) 560, 761, 1169
NdeII GATC 4 cut(s) 272, 856, 1114, 1118
NlaIII CATG 4 cut(s) 271, 572, 625, 1066
NlaIV GGNNCC 1 cut(s) 288
NmuCI GTSAC 1 cut(s) 768
NspI RCATGY 1 cut(s) 271
PaeI GCATGC 1 cut(s) 271
PctI GAATGC 1 cut(s) 143
PfeI GAWTC 3 cut(s) 167, 293, 574
PkrI GCNGC 3 cut(s) 658, 712, 1162
PleI GAGTC 1 cut(s) 209
PpsI GAGTC 1 cut(s) 209
PshBI ATTAAT 1 cut(s) 252
PsiI TTATAA 2 cut(s) 462, 635
Psp124BI GAGCTC 1 cut(s) 917
Psp6I CCWGG 1 cut(s) 589
PspFI CCCAGC 1 cut(s) 230
PspGI CCWGG 1 cut(s) 589
PspN4I GGNNCC 1 cut(s) 288
PspPI GGNCC 1 cut(s) 640
PstNI CAGNNNCTG 1 cut(s) 1112
PsuI RGATCY 1 cut(s) 856
RsaI GTAC 3 cut(s) 95, 301, 813
RsaNI GTAC 3 cut(s) 94, 300, 812
RseI CAYNNNNRTG 1 cut(s) 665
SacI GAGCTC 1 cut(s) 917
SaqAI TTAA 8 cut(s) 104, 119, 209, 252, 651, 846, 861, 870
SatI GCNGC 3 cut(s) 657, 711, 1161
Sau3AI GATC 4 cut(s) 272, 856, 1114, 1118
Sau96I GGNCC 1 cut(s) 640
SchI GAGTC 1 cut(s) 209
ScrFI CCNGG 1 cut(s) 591
SduI GDGCHC 2 cut(s) 738, 917
SfaNI GCATC 4 cut(s) 235, 550, 589, 1139
SfcI CTRYAG 1 cut(s) 949
SinI GGWCC 1 cut(s) 640
SmiMI CAYNNNNRTG 1 cut(s) 665
SmlI CTYRAG 1 cut(s) 928
SmoI CTYRAG 1 cut(s) 928
SphI GCATGC 1 cut(s) 271
Sse9I AATT 6 cut(s) 647, 871, 941, 1034, 1083, 1089
SsiI CCGC 1 cut(s) 656
SspI AATATT 2 cut(s) 151, 160
SspMI CTAG 3 cut(s) 81, 336, 966
SstI GAGCTC 1 cut(s) 917
StyD4I CCNGG 1 cut(s) 589
TaaI ACNGT 4 cut(s) 304, 616, 721, 811
TaiI ACGT 3 cut(s) 95, 1101, 1149
TaqI TCGA 1 cut(s) 1117
TasI AATT 6 cut(s) 647, 871, 941, 1034, 1083, 1089
TatI WGTACW 2 cut(s) 299, 811
TauI GCSGC 1 cut(s) 659
TfiI GAWTC 3 cut(s) 167, 293, 574
Tru1I TTAA 8 cut(s) 104, 119, 209, 252, 651, 846, 861, 870
Tru9I TTAA 8 cut(s) 104, 119, 209, 252, 651, 846, 861, 870
TseFI GTSAC 1 cut(s) 768
TseI GCWGC 2 cut(s) 710, 1160
Tsp45I GTSAC 1 cut(s) 768
TspDTI ATGAA 7 cut(s) 35, 300, 564, 599, 800, 1062, 1079
TspGWI ACGGA 3 cut(s) 292, 429, 632
VpaK11BI GGWCC 1 cut(s) 640
VspI ATTAAT 1 cut(s) 252
XapI RAATTY 2 cut(s) 647, 941
XceI RCATGY 1 cut(s) 271
XcmI CCANNNNNNNNNTGG 1 cut(s) 587
XmiI GTMKAC 2 cut(s) 500, 952
XspI CTAG 3 cut(s) 81, 336, 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.