Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0009987

Physical Location & Seq

Genomic Coordinates Forward (+)

16320213 .. 16322096

Gene Structure 1884 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1164500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1884 bp

ATGGAATCTCCACCGCCGCCGCCTTCGCTTCAAGAGCTAGACGCGGCGGAGAAGATTATTCTCCTCTGGGACTCCACGGCTTCCGAGCAGGCCCGGGAACGAATGATATTTGACGGCCAACGCGACGAAGTCGATCGCTACTTACAGGCCGTCGATGAAATCCAACGCTCCTTGTCCTCCGCCACGCTTGCCTCCGAGGATGACCAGTCCAACCTCAACTCCGCCATCCAGATCGCCATGGCTCGGTTGGAGGACGAGTTCCGCAACATCCTCCTCAACCACACCACTCCCATCGAGCCCGACTCTCTTTCCTCAGCCGATCCGAGCTCGTCAACTCACTCCGGCATCGCTGATTCGAGCCCCGAGTTCGAGGACTACTCGGCTGAAGATGACGGCGGTTTGACCACACCTAAAGCCTCCTCCGTCACCGCCGTTGACGTCGACGAGCTTCTCCAGCGCGGCGATTCGTCGAACACTAGCAGCTACAGGTCCACCAGCAGCATCCGCGAGCTCGATCTCATCCCCTCCGATGCGGTCTTCGATCTCCGCAACATCGCCGAGCGGGTGATCGCCGCCGGCTACTTACGCGAGTGTATCCAGGTGTACGGCAGCGTAAGAAAGGGGGCGGTGGACTCCAGTTTCCGGAAGCTAGGGATTGAGAAGCTGAGCATCGGAGACGTTCAGAGACTTCAGTGGGAGCAGCTGGAGACCAAGATCCGACGGTGGATACGCGCCGCCAAGGCCTGCGTGAGGACCGTCTTCGCCTCCGAGAAAAAGCTCTGCGAGCAAATCTTCAACGGCATCGGCACCGCCATTGACGATGCTTGCTTTATGGAGACAGTCAAGGGCCCCGCGATTCAGCTGTTCAACTTCGCCGAGGCCATCAGCATAAGCCGGAGGTCTCCCGAGAAGCTCTTCAAGATTCTTGACTTGCACGATGCTCTGATGGACTTGATGCCAGATATTGAATCCGTTTTCGATTCAAAATCATCGGAGTCGATTCGGATTCAGGCGGTGGAGATACTGTCGAGGCTGGCGGAGGCGGCGAGGGGGATTCTATCGGAGTTTGAGAACGCTGTGCTTCGTGAACCCTCTAGGGTTCCAGTCCCGGGCGGAACGATTCATCCATTGACTCGGTACGTGATGAATTACATCAGTTTGATATCGGATTATAAGCAGACTTTGAATGAGCTTATTGTGTCGAAGCCGTCTACAGGGTCGCGGTACTCCAGCGACCCCACGACTCCGGATATGGAGTTCGCAGAGCTAGAGGGGAAAACCCCTCTGGCTCTGCATTTGATTTGGATTATTGTGATTTTGCAATTTAATTTGGATGGCAAGTCTAAGCACTATAAGGATGCTTCAATAGCTCACTTGTTTATGATGAACAATGTTCATTACATTGTTCAAAAGGTCAAGGGGTCCCCGGAATTGAGGGAGATGATTGGAGATGATTACTTGAGGAAGTTGACCGGGAAGTTCCGCCAGGCAGCCACTAGTTACCAGAGAGCTACTTGGGTCGGGGTGTTGTATTGTTTGAGAGACGAAGGGTTACATGTGAGTGGGAGTTTTTCTTCGGGGGTGTCGAAGAGTGCATTGAGAGAGAGGTTCAAGTCTTTCAATGCTATGTTTGAGGAGGTTCATAGGACTCAAGCTACTTGGTTGATACCAGATACTCAGCTTAGGGAGGAGCTAAGGATATCTATTTCAGAGAAGTTGATCCCAGCATACAGGTCGTTCCTTGGGCGGTTTAGGAGCCATATAGAAAGTGGGAAGCATCCAGAAAATTACATCAAGTATACGACCGAGGATTTGGAGACCGCTGTATTGGATTTCTTTGAGGGGTACTCAGTATCCCAGCACTTGAGGAGGAGATCTCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006810 GO:0006887 GO:0008150 GO:0009987 GO:0016192 GO:0032940 GO:0046903 GO:0051179 GO:0051234

KEGG Pathways

Metabolic & Signaling

ko04910 map04910

Pfam Domains

Protein Families

Exo70

Protein Analysis

628

Amino Acids

70.55

Weight (kDa)

5.31

Isoelectric Point (pI)

52.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Exo70_N	PF20669	13 - 91	2.4e-14	Exocyst complex component Exo70 N-terminal
Exo70_C	PF03081	241 - 610	4.1e-131	Exo70 exocyst complex subunit C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009987)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54090 AT1G72470 AT3G14090
fragaria_vesca	FvH4_6g35110
malus_domestica	MD17G1164500.v1.1
prunus_persica	Prupe.3G038000_v2.0.a1 Prupe.3G038000_v2.0.a1
pyrus_communis	pycom09g09710 pycom17g15760
rosa_chinensis	RchiOBHm_Chr2g0146331
rosa_laevigata	RLG00000020194
rosa_multiflora	Rmu_co8454727.1_g000001 Rmu_sc0004094.1_g000002
rosa_roxburghii	Rroxscaffold_2G00100170
rosa_rugosa	Rorug02G0394100
rosa_samantha	Rh2AG450500 Rh2BG462800 Rh2CG437300 Rh2DG471900 Rh3BG270800
rosa_wichuraiana	Rw2G036770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1173
AatII	GACGTC	1 cut(s)	441
AccB1I	GGYRCC	1 cut(s)	806
AccBSI	CCGCTC	1 cut(s)	562
AccI	GTMKAC	3 cut(s)	441, 1211, 1799
AccII	CGCG	8 cut(s)	44, 123, 459, 507, 588, 732, 854, 1222
AccIII	TCCGGA	2 cut(s)	642, 1246
AclWI	GGATC	3 cut(s)	314, 709, 1714
AcoI	YGGCCR	1 cut(s)	115
AcuI	CTGAAG	2 cut(s)	405, 674
AcyI	GRCGYC	1 cut(s)	438
AfaI	GTAC	4 cut(s)	605, 1139, 1226, 1847
AfiI	CCNNNNNNNGG	5 cut(s)	243, 642, 750, 1109, 1214
AflIII	ACRYGT	1 cut(s)	1555
AhlI	ACTAGT	1 cut(s)	1496
AjnI	CCWGG	2 cut(s)	597, 1485
AloI	GAACNNNNNNTCC	6 cut(s)	242, 274, 1108, 1140, 1241, 1273
Alw21I	GWGCWC	2 cut(s)	329, 513
Alw26I	GTCTC	7 cut(s)	669, 679, 701, 830, 906, 1536, 1811
AlwI	GGATC	3 cut(s)	314, 709, 1714
Ama87I	CYCGRG	4 cut(s)	93, 362, 905, 1108
Aor13HI	TCCGGA	2 cut(s)	642, 1246
AoxI	GGCC	6 cut(s)	90, 115, 147, 741, 847, 879
ApaI	GGGCCC	1 cut(s)	851
ApeKI	GCWGC	5 cut(s)	480, 498, 609, 700, 1490
ArsI	GACNNNNNNTTYG	2 cut(s)	1795, 1827
Asp700I	GAANNNNTTC	1 cut(s)	914
AspLEI	GCGC	2 cut(s)	459, 734
AspS9I	GGNCC	6 cut(s)	91, 489, 753, 847, 848, 1422
AsuC2I	CCSGG	6 cut(s)	94, 95, 1109, 1110, 1427, 1474
AsuHPI	GGTGA	2 cut(s)	418, 577
AvaI	CYCGRG	4 cut(s)	93, 362, 905, 1108
AvaII	GGWCC	3 cut(s)	489, 753, 1422
BaeGI	GKGCMC	1 cut(s)	851
BanI	GGYRCC	1 cut(s)	806
BanII	GRGCYC	5 cut(s)	300, 329, 362, 513, 851
BbsI	GAAGAC	2 cut(s)	529, 751
Bbv12I	GWGCWC	2 cut(s)	329, 513
BbvCI	CCTCAGC	1 cut(s)	313
BbvI	GCAGC	5 cut(s)	492, 510, 621, 712, 1502
BccI	CCATC	5 cut(s)	233, 299, 890, 940, 1328
BceAI	ACGGC	8 cut(s)	93, 130, 134, 409, 416, 622, 814, 1192
BcgI	CGANNNNNNTGC	2 cut(s)	1716, 1750
BciT130I	CCWGG	2 cut(s)	599, 1487
BciVI	GTATCC	3 cut(s)	605, 720, 1864
BcnI	CCSGG	6 cut(s)	94, 95, 1109, 1110, 1427, 1474
BcoDI	GTCTC	7 cut(s)	669, 679, 701, 830, 906, 1536, 1811
BcuI	ACTAGT	1 cut(s)	1496
BfaI	CTAG	6 cut(s)	38, 477, 650, 1095, 1268, 1497
BfmI	CTRYAG	2 cut(s)	484, 1212
BfuI	GTATCC	3 cut(s)	605, 720, 1864
BglI	GCCNNNNNGGC	1 cut(s)	740
BglII	AGATCT	1 cut(s)	1874
BlpI	GCTNAGC	1 cut(s)	665
Bme1390I	CCNGG	8 cut(s)	94, 95, 599, 1109, 1110, 1427, 1474, 1487
Bme18I	GGWCC	3 cut(s)	489, 753, 1422
BmeT110I	CYCGRG	4 cut(s)	93, 362, 905, 1108
BmgT120I	GGNCC	6 cut(s)	91, 489, 753, 847, 848, 1422
BmiI	GGNNCC	7 cut(s)	808, 849, 850, 1101, 1423, 1424, 1757
BmrFI	CCNGG	8 cut(s)	94, 95, 599, 1109, 1110, 1427, 1474, 1487
BpiI	GAAGAC	2 cut(s)	529, 751
BplI	GAGNNNNNCTC	7 cut(s)	287, 319, 362, 394, 1832, 1864, 1861
BpmI	CTGGAG	4 cut(s)	437, 619, 725, 1213
Bpu10I	CCTNAGC	3 cut(s)	313, 1682, 1694
Bpu1102I	GCTNAGC	1 cut(s)	665
BpuEI	CTTGAG	2 cut(s)	1480, 1635
BpuMI	CCSGG	6 cut(s)	94, 95, 1109, 1110, 1427, 1474
BsaAI	YACGTR	1 cut(s)	1141
BsaHI	GRCGYC	1 cut(s)	438
BsaI	GGTCTC	3 cut(s)	701, 906, 1811
BsaWI	WCCGGW	2 cut(s)	642, 1246
BsaXI	ACNNNNNCTCC	6 cut(s)	203, 233, 242, 272, 1010, 1040
Bsc4I	CCNNNNNNNGG	5 cut(s)	243, 642, 750, 1109, 1214
Bse118I	RCCGGY	1 cut(s)	575
Bse1I	ACTGG	3 cut(s)	205, 636, 1103
BseAI	TCCGGA	2 cut(s)	642, 1246
BseBI	CCWGG	2 cut(s)	599, 1487
BseGI	GGATG	9 cut(s)	205, 225, 267, 501, 519, 1123, 1339, 1363, 1777
BseLI	CCNNNNNNNGG	5 cut(s)	243, 642, 750, 1109, 1214
BseMII	CTCAG	4 cut(s)	327, 656, 1691, 1863
BseNI	ACTGG	3 cut(s)	205, 636, 1103
BseRI	GAGGAG	6 cut(s)	53, 263, 409, 1649, 1703, 1882
BseSI	GKGCMC	1 cut(s)	851
BseXI	GCAGC	5 cut(s)	492, 510, 621, 712, 1502
BseYI	CCCAGC	2 cut(s)	1723, 1857
Bsh1236I	CGCG	8 cut(s)	44, 123, 459, 507, 588, 732, 854, 1222
Bsh1285I	CGRYCG	2 cut(s)	136, 1806
BshFI	GGCC	6 cut(s)	92, 117, 149, 743, 849, 881
BshNI	GGYRCC	1 cut(s)	806
BsiEI	CGRYCG	2 cut(s)	136, 1806
BsiHKAI	GWGCWC	2 cut(s)	329, 513
BsiHKCI	CYCGRG	4 cut(s)	93, 362, 905, 1108
BsiSI	CCGG	9 cut(s)	94, 342, 576, 643, 895, 1109, 1247, 1427, 1473
BslFI	GGGAC	3 cut(s)	83, 1091, 1408
BslI	CCNNNNNNNGG	5 cut(s)	243, 642, 750, 1109, 1214
BsmAI	GTCTC	7 cut(s)	669, 679, 701, 830, 906, 1536, 1811
BsmBI	CGTCTC	2 cut(s)	669, 1536
BsmFI	GGGAC	3 cut(s)	83, 1091, 1408
BsnI	GGCC	6 cut(s)	92, 117, 149, 743, 849, 881
Bso31I	GGTCTC	3 cut(s)	701, 906, 1811
BsoBI	CYCGRG	4 cut(s)	93, 362, 905, 1108
Bsp120I	GGGCCC	1 cut(s)	847
Bsp1286I	GDGCHC	5 cut(s)	300, 329, 362, 513, 851
Bsp13I	TCCGGA	2 cut(s)	642, 1246
Bsp143I	GATC	9 cut(s)	133, 231, 319, 514, 541, 567, 714, 1719, 1874
Bsp1720I	GCTNAGC	1 cut(s)	665
Bsp19I	CCATGG	1 cut(s)	237
BspANI	GGCC	6 cut(s)	92, 117, 149, 743, 849, 881
BspCNI	CTCAG	4 cut(s)	326, 657, 1690, 1862
BspEI	TCCGGA	2 cut(s)	642, 1246
BspFNI	CGCG	8 cut(s)	44, 123, 459, 507, 588, 732, 854, 1222
BspLI	GGNNCC	7 cut(s)	808, 849, 850, 1101, 1423, 1424, 1757
BspPI	GGATC	3 cut(s)	314, 709, 1714
BspQI	GCTCTTC	1 cut(s)	920
BspT107I	GGYRCC	1 cut(s)	806
BspTNI	GGTCTC	3 cut(s)	701, 906, 1811
BsrBI	CCGCTC	1 cut(s)	562
BsrFI	RCCGGY	1 cut(s)	575
BsrI	ACTGG	3 cut(s)	205, 636, 1103
BssAI	RCCGGY	1 cut(s)	575
BssMI	GATC	9 cut(s)	133, 231, 319, 514, 541, 567, 714, 1719, 1874
BssNAI	GTATAC	1 cut(s)	1800
BssNI	GRCGYC	1 cut(s)	438
BssT1I	CCWWGG	3 cut(s)	237, 738, 1741
Bst1107I	GTATAC	1 cut(s)	1800
Bst2UI	CCWGG	2 cut(s)	599, 1487
Bst4CI	ACNGT	4 cut(s)	723, 757, 841, 1026
Bst6I	CTCTTC	2 cut(s)	920, 1583
BstACI	GRCGYC	1 cut(s)	438
BstBAI	YACGTR	1 cut(s)	1141
BstC8I	GCNNGC	8 cut(s)	90, 189, 509, 577, 745, 785, 826, 1035
BstDEI	CTNAG	8 cut(s)	313, 665, 1344, 1677, 1682, 1694, 1849, 1878
BstDSI	CCRYGG	2 cut(s)	75, 237
BstENI	CCTNNNNNAGG	1 cut(s)	748
BstF5I	GGATG	9 cut(s)	205, 225, 267, 501, 519, 1123, 1339, 1363, 1777
BstFNI	CGCG	8 cut(s)	44, 123, 459, 507, 588, 732, 854, 1222
BstHHI	GCGC	2 cut(s)	459, 734
BstKTI	GATC	9 cut(s)	136, 234, 322, 517, 544, 570, 717, 1722, 1877
BstMAI	GTCTC	7 cut(s)	669, 679, 701, 830, 906, 1536, 1811
BstMBI	GATC	9 cut(s)	133, 231, 319, 514, 541, 567, 714, 1719, 1874
BstMCI	CGRYCG	2 cut(s)	136, 1806
BstNI	CCWGG	2 cut(s)	599, 1487
BstNSI	RCATGY	1 cut(s)	1559
BstSCI	CCNGG	8 cut(s)	92, 93, 597, 1107, 1108, 1425, 1472, 1485
BstSFI	CTRYAG	2 cut(s)	484, 1212
BstSLI	GKGCMC	1 cut(s)	851
BstUI	CGCG	8 cut(s)	44, 123, 459, 507, 588, 732, 854, 1222
BstV1I	GCAGC	5 cut(s)	492, 510, 621, 712, 1502
BstV2I	GAAGAC	2 cut(s)	529, 751
BstX2I	RGATCY	2 cut(s)	714, 1874
BstYI	RGATCY	2 cut(s)	714, 1874
BstZ17I	GTATAC	1 cut(s)	1800
BsuI	GTATCC	3 cut(s)	605, 720, 1864
BsuRI	GGCC	6 cut(s)	92, 117, 149, 743, 849, 881
BtgI	CCRYGG	2 cut(s)	75, 237
BtgZI	GCGATG	2 cut(s)	331, 538
BtsCI	GGATG	9 cut(s)	205, 225, 267, 501, 519, 1123, 1339, 1363, 1777
BtsIMutI	CAGTG	1 cut(s)	698
Cac8I	GCNNGC	8 cut(s)	90, 189, 509, 577, 745, 785, 826, 1035
CfoI	GCGC	2 cut(s)	459, 734
Cfr10I	RCCGGY	1 cut(s)	575
Cfr13I	GGNCC	6 cut(s)	91, 489, 753, 847, 848, 1422
Cfr9I	CCCGGG	2 cut(s)	93, 1108
CseI	GACGC	1 cut(s)	50
Csp6I	GTAC	4 cut(s)	604, 1138, 1225, 1846
CviAII	CATG	2 cut(s)	238, 1556
CviQI	GTAC	4 cut(s)	604, 1138, 1225, 1846
DdeI	CTNAG	8 cut(s)	313, 665, 1344, 1677, 1682, 1694, 1849, 1878
DpnI	GATC	9 cut(s)	135, 233, 321, 516, 543, 569, 716, 1721, 1876
DpnII	GATC	9 cut(s)	133, 231, 319, 514, 541, 567, 714, 1719, 1874
EaeI	YGGCCR	1 cut(s)	115
Eam1104I	CTCTTC	2 cut(s)	920, 1583
EarI	CTCTTC	2 cut(s)	920, 1583
EciI	GGCGGA	6 cut(s)	62, 169, 211, 1052, 1128, 1472
Ecl136II	GAGCTC	2 cut(s)	327, 511
Eco130I	CCWWGG	3 cut(s)	237, 738, 1741
Eco147I	AGGCCT	1 cut(s)	743
Eco24I	GRGCYC	5 cut(s)	300, 329, 362, 513, 851
Eco31I	GGTCTC	3 cut(s)	701, 906, 1811
Eco32I	GATATC	2 cut(s)	1164, 1701
Eco47I	GGWCC	3 cut(s)	489, 753, 1422
Eco53kI	GAGCTC	2 cut(s)	327, 511
Eco57I	CTGAAG	2 cut(s)	405, 674
Eco88I	CYCGRG	4 cut(s)	93, 362, 905, 1108
EcoICRI	GAGCTC	2 cut(s)	327, 511
EcoNI	CCTNNNNNAGG	1 cut(s)	748
EcoO109I	RGGNCCY	3 cut(s)	847, 848, 1422
EcoRII	CCWGG	2 cut(s)	597, 1485
EcoRV	GATATC	2 cut(s)	1164, 1701
EcoT14I	CCWWGG	3 cut(s)	237, 738, 1741
EcoT38I	GRGCYC	5 cut(s)	300, 329, 362, 513, 851
ErhI	CCWWGG	3 cut(s)	237, 738, 1741
Esp3I	CGTCTC	2 cut(s)	669, 1536
FaeI	CATG	2 cut(s)	241, 1559
FaqI	GGGAC	3 cut(s)	83, 1091, 1408
FatI	CATG	2 cut(s)	237, 1555
FauI	CCCGC	2 cut(s)	555, 859
FblI	GTMKAC	3 cut(s)	441, 1211, 1799
FokI	GGATG	9 cut(s)	212, 212, 254, 488, 506, 1110, 1346, 1370, 1764
FriOI	GRGCYC	5 cut(s)	300, 329, 362, 513, 851
FspBI	CTAG	6 cut(s)	38, 477, 650, 1095, 1268, 1497
GlaI	GCGC	2 cut(s)	458, 733
GsaI	CCCAGC	2 cut(s)	1727, 1861
GsuI	CTGGAG	4 cut(s)	437, 619, 725, 1213
HaeIII	GGCC	6 cut(s)	92, 117, 149, 743, 849, 881
HapII	CCGG	9 cut(s)	94, 342, 576, 643, 895, 1109, 1247, 1427, 1473
HgaI	GACGC	1 cut(s)	50
HhaI	GCGC	2 cut(s)	459, 734
Hin1I	GRCGYC	1 cut(s)	438
Hin1II	CATG	2 cut(s)	241, 1559
Hin6I	GCGC	2 cut(s)	457, 732
HinP1I	GCGC	2 cut(s)	457, 732
HincII	GTYRAC	4 cut(s)	333, 436, 442, 1470
HindII	GTYRAC	4 cut(s)	333, 436, 442, 1470
HpaII	CCGG	9 cut(s)	94, 342, 576, 643, 895, 1109, 1247, 1427, 1473
HphI	GGTGA	2 cut(s)	418, 577
Hpy188III	TCNNGA	9 cut(s)	32, 229, 643, 905, 919, 926, 1085, 1247, 1781
Hpy99I	CGWCG	6 cut(s)	128, 155, 443, 446, 472, 723
HpyAV	CCTTC	2 cut(s)	33, 1541
HpyCH4III	ACNGT	4 cut(s)	723, 757, 841, 1026
HpyCH4IV	ACGT	3 cut(s)	438, 678, 1140
HpyCH4V	TGCA	4 cut(s)	934, 1294, 1321, 1595
HpyF3I	CTNAG	8 cut(s)	313, 665, 1344, 1677, 1682, 1694, 1849, 1878
HpySE526I	ACGT	3 cut(s)	438, 678, 1140
Hsp92I	GRCGYC	1 cut(s)	438
Hsp92II	CATG	2 cut(s)	241, 1559
HspAI	GCGC	2 cut(s)	457, 732
KflI	GGGWCCC	1 cut(s)	1422
Kpn2I	TCCGGA	2 cut(s)	642, 1246
KroI	GCCGGC	1 cut(s)	575
KroNI	GCCGGC	1 cut(s)	577
Kzo9I	GATC	9 cut(s)	133, 231, 319, 514, 541, 567, 714, 1719, 1874
LguI	GCTCTTC	1 cut(s)	920
LmnI	GCTCC	4 cut(s)	173, 697, 1690, 1755
Lsp1109I	GCAGC	5 cut(s)	492, 510, 621, 712, 1502
MaeI	CTAG	6 cut(s)	38, 477, 650, 1095, 1268, 1497
MaeII	ACGT	3 cut(s)	438, 678, 1140
MaeIII	GTNAC	3 cut(s)	424, 1499, 1551
MalI	GATC	9 cut(s)	135, 233, 321, 516, 543, 569, 716, 1721, 1876
MbiI	CCGCTC	1 cut(s)	562
MboI	GATC	9 cut(s)	133, 231, 319, 514, 541, 567, 714, 1719, 1874
MboII	GAAGA	8 cut(s)	64, 398, 529, 751, 784, 907, 1566, 1600
MflI	RGATCY	2 cut(s)	714, 1874
MhlI	GDGCHC	5 cut(s)	300, 329, 362, 513, 851
MluCI	AATT	5 cut(s)	1147, 1322, 1327, 1430, 1786
MlyI	GAGTC	7 cut(s)	65, 296, 626, 1004, 1126, 1237, 1642
MmeI	TCCRAC	4 cut(s)	187, 228, 234, 742
MroI	TCCGGA	2 cut(s)	642, 1246
MroNI	GCCGGC	1 cut(s)	575
MroXI	GAANNNNTTC	1 cut(s)	914
MseI	TTAA	1 cut(s)	1326
MslI	CAYNNNNRTG	1 cut(s)	1560
MspA1I	CMGCKG	3 cut(s)	703, 862, 1823
MspI	CCGG	9 cut(s)	94, 342, 576, 643, 895, 1109, 1247, 1427, 1473
MspR9I	CCNGG	8 cut(s)	94, 95, 599, 1109, 1110, 1427, 1474, 1487
MvaI	CCWGG	2 cut(s)	599, 1487
MvnI	CGCG	8 cut(s)	44, 123, 459, 507, 588, 732, 854, 1222
NaeI	GCCGGC	1 cut(s)	577
NciI	CCSGG	6 cut(s)	94, 95, 1109, 1110, 1427, 1474
NcoI	CCATGG	1 cut(s)	237
NdeII	GATC	9 cut(s)	133, 231, 319, 514, 541, 567, 714, 1719, 1874
NgoMIV	GCCGGC	1 cut(s)	575
NlaIII	CATG	2 cut(s)	241, 1559
NlaIV	GGNNCC	7 cut(s)	808, 849, 850, 1101, 1423, 1424, 1757
NmeAIII	GCCGAG	3 cut(s)	359, 583, 901
NmuCI	GTSAC	1 cut(s)	424
NspI	RCATGY	1 cut(s)	1559
PceI	AGGCCT	1 cut(s)	743
PciI	ACATGT	1 cut(s)	1555
PciSI	GCTCTTC	1 cut(s)	920
PcsI	WCGNNNNNNNCGW	2 cut(s)	120, 1583
PdiI	GCCGGC	1 cut(s)	577
PdmI	GAANNNNTTC	1 cut(s)	914
PflFI	GACNNNGTC	1 cut(s)	128
Ple19I	CGATCG	1 cut(s)	136
PleI	GAGTC	7 cut(s)	65, 296, 626, 1003, 1126, 1237, 1642
PpsI	GAGTC	7 cut(s)	65, 296, 626, 1003, 1126, 1237, 1642
Ppu21I	YACGTR	1 cut(s)	1141
PpuMI	RGGWCCY	1 cut(s)	1422
PscI	ACATGT	1 cut(s)	1555
PsiI	TTATAA	1 cut(s)	1173
Psp124BI	GAGCTC	2 cut(s)	329, 513
Psp5II	RGGWCCY	1 cut(s)	1422
Psp6I	CCWGG	2 cut(s)	597, 1485
PspFI	CCCAGC	2 cut(s)	1723, 1857
PspGI	CCWGG	2 cut(s)	597, 1485
PspN4I	GGNNCC	7 cut(s)	808, 849, 850, 1101, 1423, 1424, 1757
PspOMI	GGGCCC	1 cut(s)	847
PspPI	GGNCC	6 cut(s)	91, 489, 753, 847, 848, 1422
PspPPI	RGGWCCY	1 cut(s)	1422
PsuI	RGATCY	2 cut(s)	714, 1874
PsyI	GACNNNGTC	1 cut(s)	128
PvuI	CGATCG	1 cut(s)	136
PvuII	CAGCTG	2 cut(s)	703, 862
RsaI	GTAC	4 cut(s)	605, 1139, 1226, 1847
RsaNI	GTAC	4 cut(s)	604, 1138, 1225, 1846
RseI	CAYNNNNRTG	1 cut(s)	1560
SacI	GAGCTC	2 cut(s)	329, 513
SalI	GTCGAC	1 cut(s)	440
SapI	GCTCTTC	1 cut(s)	920
SaqAI	TTAA	1 cut(s)	1326
Sau3AI	GATC	9 cut(s)	133, 231, 319, 514, 541, 567, 714, 1719, 1874
Sau96I	GGNCC	6 cut(s)	91, 489, 753, 847, 848, 1422
SchI	GAGTC	7 cut(s)	65, 296, 626, 1004, 1126, 1237, 1642
ScrFI	CCNGG	8 cut(s)	94, 95, 599, 1109, 1110, 1427, 1474, 1487
SduI	GDGCHC	5 cut(s)	300, 329, 362, 513, 851
SfcI	CTRYAG	2 cut(s)	484, 1212
SgrDI	CGTCGACG	1 cut(s)	440
SinI	GGWCC	3 cut(s)	489, 753, 1422
SmaI	CCCGGG	2 cut(s)	95, 1110
SmiMI	CAYNNNNRTG	1 cut(s)	1560
SmlI	CTYRAG	3 cut(s)	1459, 1650, 1864
SmoI	CTYRAG	3 cut(s)	1459, 1650, 1864
SpeI	ACTAGT	1 cut(s)	1496
Sse9I	AATT	5 cut(s)	1147, 1322, 1327, 1430, 1786
SseBI	AGGCCT	1 cut(s)	743
SspMI	CTAG	6 cut(s)	38, 477, 650, 1095, 1268, 1497
SstI	GAGCTC	2 cut(s)	329, 513
StuI	AGGCCT	1 cut(s)	743
StyD4I	CCNGG	8 cut(s)	92, 93, 597, 1107, 1108, 1425, 1472, 1485
StyI	CCWWGG	3 cut(s)	237, 738, 1741
TaaI	ACNGT	4 cut(s)	723, 757, 841, 1026
TaiI	ACGT	3 cut(s)	441, 681, 1143
TaqII	GACCGA	1 cut(s)	1820
TasI	AATT	5 cut(s)	1147, 1322, 1327, 1430, 1786
TauI	GCSGC	7 cut(s)	19, 22, 47, 462, 575, 737, 1046
Tru1I	TTAA	1 cut(s)	1326
Tru9I	TTAA	1 cut(s)	1326
TscAI	CASTG	1 cut(s)	698
TseFI	GTSAC	1 cut(s)	424
TseI	GCWGC	5 cut(s)	480, 498, 609, 700, 1490
Tsp45I	GTSAC	1 cut(s)	424
TspDTI	ATGAA	6 cut(s)	171, 1112, 1160, 1385, 1400, 1631
TspGWI	ACGGA	2 cut(s)	412, 961
TspMI	CCCGGG	2 cut(s)	93, 1108
TspRI	CASTG	1 cut(s)	698
Tth111I	GACNNNGTC	1 cut(s)	128
VpaK11BI	GGWCC	3 cut(s)	489, 753, 1422
XagI	CCTNNNNNAGG	1 cut(s)	748
XceI	RCATGY	1 cut(s)	1559
XcmI	CCANNNNNNNNNTGG	2 cut(s)	244, 1766
XmaI	CCCGGG	2 cut(s)	93, 1108
XmiI	GTMKAC	3 cut(s)	441, 1211, 1799
XmnI	GAANNNNTTC	1 cut(s)	914
XspI	CTAG	6 cut(s)	38, 477, 650, 1095, 1268, 1497
ZraI	GACGTC	1 cut(s)	439

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.